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Cryomicroscopic Investigations Of Freezing Processes In Cell Suspensions, Tathagata Acharya, Ram V. Devireddy Nov 2010

Cryomicroscopic Investigations Of Freezing Processes In Cell Suspensions, Tathagata Acharya, Ram V. Devireddy

Faculty Publications

This study evaluates the freezing response of three different cell types, Pacific oyster embryos (∼50 m in diameter), Jurkat cells and HeLa cells (∼12 to 15 m's in diameter), using cryomicroscopy. Freezing experiments were performed on oyster embryos at cooling rates of either 5 or 10 °C/min, while Jurkats were cooled at either 1 or 10 °C/min and HeLa cells were cooled at either 1, 15 or 20 °C/min, respectively. The experiments with oyster embryos were primarily designed to investigate the phenomena of intracellular ice formation (IIF) while the experiments for Jurkat and HeLa cells were designed to investigate both …

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New Insights Into The Organization, Recombination, Expression And Functional Mechanism Of Low Molecular Weight Glutenin Subunit Genes In Bread Wheat, Lingli Dong, Xiaofei Zhang, Dongcheng Liu, Huajie Fan, Jiazhu Sun, Zhongjuan Zhang, Huanju Qin, Bin Li, Shanting Hao, Zhensheng Li, Daowen Wang, Aimin Zhang, Hong Qing Ling Nov 2010

New Insights Into The Organization, Recombination, Expression And Functional Mechanism Of Low Molecular Weight Glutenin Subunit Genes In Bread Wheat, Lingli Dong, Xiaofei Zhang, Dongcheng Liu, Huajie Fan, Jiazhu Sun, Zhongjuan Zhang, Huanju Qin, Bin Li, Shanting Hao, Zhensheng Li, Daowen Wang, Aimin Zhang, Hong Qing Ling

Faculty Publications

The bread-making quality of wheat is strongly influenced by multiple low molecular weight glutenin subunit (LMW-GS) proteins expressed in the seeds. However, the organization, recombination and expression of LMW-GS genes and their functional mechanism in bread-making are not well understood. Here we report a systematic molecular analysis of LMW-GS genes located at the orthologous Glu-3 loci (Glu-A3, B3 and D3) of bread wheat using complementary approaches (genome wide characterization of gene members, expression profiling, proteomic analysis). Fourteen unique LMW-GS genes were identified for Xiaoyan 54 (with superior bread-making quality). Molecular mapping and recombination analyses revealed that the three Glu-3 loci …

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Genetic Diversity In A Deep-Sea Harpacticoid Copepod Found Near Two Oil-Drilling Sites In The Gulf Of Mexico, Christopher S. Gregg, David W. Foltz, John W. Fleeger Nov 2010

Genetic Diversity In A Deep-Sea Harpacticoid Copepod Found Near Two Oil-Drilling Sites In The Gulf Of Mexico, Christopher S. Gregg, David W. Foltz, John W. Fleeger

Faculty Publications

Sites adjacent to (near-field) and distant from (far-field) oil-drilling platforms were sampled for harpacticoid copepods at two locations in the northern Gulf of Mexico off the coast of Louisiana, both at depths of ≈ 1100 m. The sites were located at Garden Banks Area Block 602 (GB 602) and Mississippi Canyon Area Block 292 (MC 292). Near-field sites at both locations were characterized by large numbers of a single undescribed species of harpacticoid copepod, Bathycletopsyllus sp., but this species was essentially absent from far-field sites. We sequenced a 710 bp portion of the mitochondrial cytochrome c oxidase subunit I gene …

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Lines And Sines Of Primate Evolution, Miriam K. Konkel, Jerilyn A. Walker, Mark A. Batzer Nov 2010

Lines And Sines Of Primate Evolution, Miriam K. Konkel, Jerilyn A. Walker, Mark A. Batzer

Faculty Publications

The primate order is a monophyletic group thought to have diverged from the Euarchonta more than 65 mya.1 Recent paleontological and molecular evolution studies place the last common ancestor of primates even earlier (≥ 85 mya).2 More than 300 extant primate species are recognized today,3, 4 clearly emphasizing their diversity and success. Our understanding of the evolution of primates and the composition of their genomes has been revolutionized within the last decade through the increasing availability and analyses of sequenced genomes. However, several aspects of primate evolution have yet to be resolved. DNA sequencing of a wider array of primate …

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Development Of Microsatellites For Genetic Analyses And Population Assignment Of The Cat Flea (Siphonaptera: Pulicidae), Claudia Husseneder, Susan P. Garner, Lane D. Foil, Kevin R. Macaluso Nov 2010

Development Of Microsatellites For Genetic Analyses And Population Assignment Of The Cat Flea (Siphonaptera: Pulicidae), Claudia Husseneder, Susan P. Garner, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Cat fleas, Ctenocephalides felis (Bouch) (Siphonaptera: Pulicidae), are common ectoparasites of companion animals that negatively impact their hosts directly by causing dermatitis and blood loss during feeding and indirectly through the potential transmission of disease causing agents. We isolated and characterized seven novel microsatellite loci from a partial genomic library of the cat flea enriched for di-, tri-, and tetranucleotide repeats. We screened these loci in cat fleas from two laboratory colonies and one wild-caught population collected at a temporary animal shelter (Parker coliseum) in Baton Rouge, LA. Six loci were polymorphic, with two to 15 alleles per locus and …

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Genome Structures And Halophyte-Specific Gene Expression Of The Extremophile Thellungiella Parvula In Comparison With Thellungiella Salsuginea (Thellungiella Halophila) And Arabidopsis, Dong Ha Oh, Maheshi Dassanayake, Jeffrey S. Haas, Anna Kropornika, Chris Wright, Matilde Paino D'Urzo, Hyewon Hong, Shahjahan Ali, Alvaro Hernandez, Georgina M. Lambert, Gunsu Inan, David W. Galbraith, Ray A. Bressan, Dae Jin Yun, Jian Kang Zhu, John M. Cheeseman, Hans J. Bohnert Nov 2010

Genome Structures And Halophyte-Specific Gene Expression Of The Extremophile Thellungiella Parvula In Comparison With Thellungiella Salsuginea (Thellungiella Halophila) And Arabidopsis, Dong Ha Oh, Maheshi Dassanayake, Jeffrey S. Haas, Anna Kropornika, Chris Wright, Matilde Paino D'Urzo, Hyewon Hong, Shahjahan Ali, Alvaro Hernandez, Georgina M. Lambert, Gunsu Inan, David W. Galbraith, Ray A. Bressan, Dae Jin Yun, Jian Kang Zhu, John M. Cheeseman, Hans J. Bohnert

Faculty Publications

The genome of Thellungiella parvula, a halophytic relative of Arabidopsis (Arabidopsis thaliana), is being assembled using Roche-454 sequencing. Analyses of a 10-Mb scaffold revealed synteny with Arabidopsis, with recombination and inversion and an uneven distribution of repeat sequences. T. parvula genome structure and DNA sequences were compared with orthologous regions from Arabidopsis and publicly available bacterial artificial chromosome sequences from Thellungiella salsuginea (previously Thellungiella halophila). The three-way comparison of sequences, from one abiotic stress-sensitive species and two tolerant species, revealed extensive sequence conservation and microcolinearity, but grouping Thellungiella species separately from Arabidopsis. However, the T. parvula segments are distinguished from …

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Assessment Of Glycoprotein Interactions With 4-[(2-Aminoethyl)Carbamoyl] Phenylboronic Acid Surfaces Using Surface Plasmon Resonance Spectroscopy, Jennifer Mac Alindong De Guzman, Steven A. Soper, Robin L. Mccarley Nov 2010

Assessment Of Glycoprotein Interactions With 4-[(2-Aminoethyl)Carbamoyl] Phenylboronic Acid Surfaces Using Surface Plasmon Resonance Spectroscopy, Jennifer Mac Alindong De Guzman, Steven A. Soper, Robin L. Mccarley

Faculty Publications

Reported here are analyses of the interactions between a select group of solution-phase glycoproteins and a unique boronic acid capture surface. The boronic acid derivative, 4-[(2-aminoethyl)carbamoyl]phenylboronic acid, AECPBA, was synthesized and then immobilized on carboxymethyl dextran surfaces using simple coupling methods. From surface plasmon resonance spectroscopy responses, it is found that model glycoproteins interact strongly with the AECPBA surface and subsequently can be readily released from the AECPBA surface using borate buffer. A striking difference between the glycoproteins fetuin and asialofetuin (desialylated fetuin), in terms of glycoprotein binding to the AECPBA surface, indicates that the interaction of glycoproteins with the …

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Sex-Differences In Adiponectin Levels And Body Fat Distribution: Longitudinal Observations In Afro-Jamaicans, Michael S. Boyne, Nadia R. Bennett, Richard S. Cooper, Tamika Y. Royal-Thomas, Franklyn I. Bennett, Amy Luke, Rainford J. Wilks, Terrence E. Forrester Nov 2010

Sex-Differences In Adiponectin Levels And Body Fat Distribution: Longitudinal Observations In Afro-Jamaicans, Michael S. Boyne, Nadia R. Bennett, Richard S. Cooper, Tamika Y. Royal-Thomas, Franklyn I. Bennett, Amy Luke, Rainford J. Wilks, Terrence E. Forrester

Faculty Publications

We longitudinally explored the relationship of body size and adiponectin levels in 393 community-dwelling Afro-Jamaicans. Adiponectin levels were greater in women, increased with age and declined with abdominal adiposity. Multivariate regression analyses suggest that subcutaneous fat in women may contribute significantly to the variance in their adiponectin levels. © 2010 Elsevier Ireland Ltd.

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Behavioral Flexibility And Species Invasions: The Adaptive Flexibility Hypothesis, T. F. Wright, J. R. Eberhard, E. A. Hobson, M. L. Avery, M. A. Russello Nov 2010

Behavioral Flexibility And Species Invasions: The Adaptive Flexibility Hypothesis, T. F. Wright, J. R. Eberhard, E. A. Hobson, M. L. Avery, M. A. Russello

Faculty Publications

Behavioral flexibility is an important adaptive response to changing environments for many animal species. Such plasticity may also promote the invasion of novel habitats by introduced species by providing them with the ability to expand or change their ecological niche, a longstanding idea with recent empirical support. At the individual level, flexibility may arise through innovation, in which an individual invents a new behavior, or through social learning, in which an individual adopts a behavior used by others. There is increasing evidence that the adaptive value of these two modes of learning, and the overall expression of behavioral flexibility, may …

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Effects Of Hurricane Katrina And Salvage Logging On Bachman's Sparrow, Matthew E. Brooks, Philip C. Stouffer Nov 2010

Effects Of Hurricane Katrina And Salvage Logging On Bachman's Sparrow, Matthew E. Brooks, Philip C. Stouffer

Faculty Publications

In the southeastern U. S., longleaf pine savannas, important habitat for grassland birds, are subject to hurricanes and subsequent management. The intensity of tropical storms is predicted to increase with climate change, heightening the importance of understanding the effects of storm disturbance and salvage logging on species of concern such as Bachman's Sparrow (Peucaea aestivalis), a pine-savanna specialist. To determine how Bachman's Sparrow occupancy varies with the age and salvage history of pine stands, and to identify the vegetation and post-hurricane habitat features influencing stand occupancy, we surveyed Bachman's Sparrows in Mississippi savannas affected by Hurricane Katrina. Our point counts …

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Regulation Of Integrin Αiibβ3 Ligand Binding And Signaling By The Metal Ion Binding Sites In The Β I Domain, Joel Raborn Nov 2010

Regulation Of Integrin Αiibβ3 Ligand Binding And Signaling By The Metal Ion Binding Sites In The Β I Domain, Joel Raborn

Honors Capstones

No abstract provided.

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A Map Of Human Genome Variation From Population-Scale Sequencing, David L. Altshuler, Richard M. Durbin, Gonçalo R. Abecasis, David R. Bentley, Aravinda Chakravarti, Andrew G. Clark, Francis S. Collins, Francisco M. De La Vega, Peter Donnelly, Michael Egholm, Paul Flicek, Stacey B. Gabriel, Richard A. Gibbs, Bartha M. Knoppers, Eric S. Lander, Hans Lehrach, Elaine R. Mardis, Gil A. Mcvean, Debbie A. Nickerson, Leena Peltonen, Alan J. Schafer, Stephen T. Sherry, Jun Wang, Richard K. Wilson, David Deiros, Mike Metzker, Donna Muzny, Jeff Reid, David Wheeler, Shenzhen Jun Wang, Jingxiang Li, Min Jian, Guoqing Li, Ruiqiang Li Oct 2010

A Map Of Human Genome Variation From Population-Scale Sequencing, David L. Altshuler, Richard M. Durbin, Gonçalo R. Abecasis, David R. Bentley, Aravinda Chakravarti, Andrew G. Clark, Francis S. Collins, Francisco M. De La Vega, Peter Donnelly, Michael Egholm, Paul Flicek, Stacey B. Gabriel, Richard A. Gibbs, Bartha M. Knoppers, Eric S. Lander, Hans Lehrach, Elaine R. Mardis, Gil A. Mcvean, Debbie A. Nickerson, Leena Peltonen, Alan J. Schafer, Stephen T. Sherry, Jun Wang, Richard K. Wilson, David Deiros, Mike Metzker, Donna Muzny, Jeff Reid, David Wheeler, Shenzhen Jun Wang, Jingxiang Li, Min Jian, Guoqing Li, Ruiqiang Li

Faculty Publications

The 1000 Genomes Project aims to provide a deep characterization of human genome sequence variation as a foundation for investigating the relationship between genotype and phenotype. Here we present results of the pilot phase of the project, designed to develop and compare different strategies for genome-wide sequencing with high-throughput platforms. We undertook three projects: low-coverage whole-genome sequencing of 179 individuals from four populations; high-coverage sequencing of two mother-father-child trios; and exon-targeted sequencing of 697 individuals from seven populations. We describe the location, allele frequency and local haplotype structure of approximately 15 million single nucleotide polymorphisms, 1 million short insertions and …

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Dated Molecular Phylogenies Indicate A Miocene Origin For Arabidopsis Thaliana, Mark A. Beilstein, Nathalie S. Nagalingum, Mark D. Clements, Steven R. Manchester, Sarah Mathews Oct 2010

Dated Molecular Phylogenies Indicate A Miocene Origin For Arabidopsis Thaliana, Mark A. Beilstein, Nathalie S. Nagalingum, Mark D. Clements, Steven R. Manchester, Sarah Mathews

Faculty Publications

Dated molecular phylogenies are the basis for understanding species diversity and for linking changes in rates of diversification with historical events such as restructuring in developmental pathways, genome doubling, or dispersal onto a new continent. Valid fossil calibration points are essential to the accurate estimation of divergence dates, but for many groups of flowering plants fossil evidence is unavailable or limited. Arabidopsis thaliana, the primary genetic model in plant biology and the first plant to have its entire genome sequenced, belongs to one such group, the plant family Brassicaceae. Thus, the timing of A. thaliana evolution and the history of …

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Comprehensive Structural And Functional Characterization Of The Human Kinome By Protein Structure Modeling And Ligand Virtual Screening, Michal Brylinski, Jeffrey Skolnick Oct 2010

Comprehensive Structural And Functional Characterization Of The Human Kinome By Protein Structure Modeling And Ligand Virtual Screening, Michal Brylinski, Jeffrey Skolnick

Faculty Publications

The growing interest in the identification of kinase inhibitors, promising therapeutics in the treatment of many diseases, has created a demand for the structural characterization of the entire human kinome. At the outset of the drug development process, the lead-finding stage, approaches that enrich the screening library with bioactive compounds are needed. Here, protein structure based methods can play an important role, but despite structural genomics efforts, it is unlikely that the three-dimensional structures of the entire kinome will be available soon. Therefore, at the proteome level, structure-based approaches must rely on predicted models, with a key issue being their …

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The Enigmatic Ichnofossil Tisoa Siphonalis And Widespread Authigenic Seep Carbonate Formation During The Late Pliensbachian In Southern France, B. Van De Schootbrugge, D. Harazim, K. Sorichter, W. Oschmann, J. Fiebig, W. Püttmann, M. Peinl, F. Zanella, B. M.A. Teichert, J. Hoffmann, A. Stadnitskaia, Y. Rosenthal Oct 2010

The Enigmatic Ichnofossil Tisoa Siphonalis And Widespread Authigenic Seep Carbonate Formation During The Late Pliensbachian In Southern France, B. Van De Schootbrugge, D. Harazim, K. Sorichter, W. Oschmann, J. Fiebig, W. Püttmann, M. Peinl, F. Zanella, B. M.A. Teichert, J. Hoffmann, A. Stadnitskaia, Y. Rosenthal

Faculty Publications

Tubular carbonate concretions of up to 1 m in length and perpendicular to bedding, occur abundantly in the Upper Pliensbachian (upper Amaltheus margaritatus Zone, Gibbosus Subzone) in outcrops (Fontaneilles section) in the vicinity of Riviére-sûr-Tarn, southern France. Stable isotope analyses of these concretions show negative δ13C values that decrease from the rim to the center from -18.8‰ to -25.7‰ (V-PDB), but normal marine δ18O values (-1.8‰). Carbon isotope analyses of Late Pliensbachian bulk carbonate (matrix) samples from the Fontaneilles section show clearly decreasing C-isotope values across the A. margaritatus Zone, from +1‰ to -3‰ (V-PDB). Isotope analyses of coeval belemnite …

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The Sll0606 Protein Is Required For Photosystem Ii Assembly/Stability In The Cyanobacterium Synechocystis Sp. Pcc 6803, Shulu Zhang, Laurie K. Frankel, Terry M. Bricker Oct 2010

The Sll0606 Protein Is Required For Photosystem Ii Assembly/Stability In The Cyanobacterium Synechocystis Sp. Pcc 6803, Shulu Zhang, Laurie K. Frankel, Terry M. Bricker

Faculty Publications

An insertional transposon mutation in the sll0606 gene was found to lead to a loss of photoautotrophy but not photoheterotrophy in the cyanobacterium Synechocystis sp. PCC 6803. Complementation analysis of this mutant (Tsll0606) indicated that an intact sll0606 gene could fully restore photoautotrophic growth. Gene organization in the vicinity of sll0606 indicates that it is not contained in an operon. No electron transport activity was detected in Tsll0606 using water as an electron donor and 2,6-dichlorobenzoquinone as an electron acceptor, indicating that Photosystem II (PS II) was defective. Electron transport activity using dichlorophenol indolephenol plus ascorbate as an electron donor …

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The Effect Of Typhoon On Particulate Organic Carbon Flux In The Southern East China Sea, C. C. Hung, G. C. Gong, W. C. Chou, C. C. Chung, M. A. Lee, Y. Chang, H. Y. Chen, S. J. Huang, Y. Yang, W. R. Yang, W. C. Chung, W. C. Li, E. Laws Oct 2010

The Effect Of Typhoon On Particulate Organic Carbon Flux In The Southern East China Sea, C. C. Hung, G. C. Gong, W. C. Chou, C. C. Chung, M. A. Lee, Y. Chang, H. Y. Chen, S. J. Huang, Y. Yang, W. R. Yang, W. C. Chung, W. C. Li, E. Laws

Faculty Publications

Severe tropical storms play an important role in triggering phytoplankton blooms, but the extent to which such storms influence biogenic carbon flux from the euphotic zone is unclear. In 2008, typhoon Fengwong provided a unique opportunity to study the in situ biological responses including phytoplankton blooms and particulate organic carbon fluxes associated with a severe storm in the southern East China Sea (SECS). After passage of the typhoon, the sea surface temperature (SST) in the SECS was markedly cooler (∼25 to 26 °C) than before typhoon passage (∼28 to 29 °C). The POC flux 5 days after passage of the …

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Multiple Ca2+-Dependent Mechanisms Regulate L-Type Ca 2+ Current In Retinal Amacrine Cells, Merve Tekmen, Evanna Gleason Oct 2010

Multiple Ca2+-Dependent Mechanisms Regulate L-Type Ca 2+ Current In Retinal Amacrine Cells, Merve Tekmen, Evanna Gleason

Faculty Publications

Understanding the regulation of L-type voltage-gated Ca2+ current is an important component of elucidating the signaling capabilities of retinal amacrine cells. Here we ask how the cytosolic Ca2+ environment and the balance of Ca2+-dependent effectors shape native L-type Ca2+ channel function in these cells. To achieve this, whole cell voltage clamp recordings were made from cultured amacrine cells under conditions that address the contribution of mitochondrial Ca2+ uptake (MCU), Ca2+/calmodulin (CaM)-dependent channel inactivation (CDI), protein kinase A (PKA), and Ca2+-induced Ca2+ release (CICR). Under control conditions, repeated activation of the L-type channels produces a progressive enhancement of the current. Inhibition …

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Hras1 And Lass1 With Apoe Are Associated With Human Longevity And Healthy Aging, S. Michal Jazwinski, Sangkyu Kim, Jianliang Dai, Li Li, Xiuhua Bi, James C. Jiang, Jonathan Arnold, Mark A. Batzer, Jerilyn A. Walker, David A. Welsh, Christina M. Lefante, Julia Volaufova, Leann Myers, L. Joseph Su, Dorothy B. Hausman, Michael V. Miceli, Eric Ravussin, Leonard W. Poon, Katie E. Cherry, Michael A. Welsch Oct 2010

Hras1 And Lass1 With Apoe Are Associated With Human Longevity And Healthy Aging, S. Michal Jazwinski, Sangkyu Kim, Jianliang Dai, Li Li, Xiuhua Bi, James C. Jiang, Jonathan Arnold, Mark A. Batzer, Jerilyn A. Walker, David A. Welsh, Christina M. Lefante, Julia Volaufova, Leann Myers, L. Joseph Su, Dorothy B. Hausman, Michael V. Miceli, Eric Ravussin, Leonard W. Poon, Katie E. Cherry, Michael A. Welsch

Faculty Publications

The search for longevity-determining genes in human has largely neglected the operation of genetic interactions. We have identified a novel combination of common variants of three genes that has a marked association with human lifespan and healthy aging. Subjects were recruited and stratified according to their genetically inferred ethnic affiliation to account for population structure. Haplotype analysis was performed in three candidate genes, and the haplotype combinations were tested for association with exceptional longevity. An HRAS1 haplotype enhanced the effect of an APOE haplotype on exceptional survival, and a LASS1 haplotype further augmented its magnitude. These results were replicated in …

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Louisiana Agriculture Fall, 2010 Oct 2010

Louisiana Agriculture Fall, 2010

Louisiana Agriculture

No abstract provided.

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Genetypes: A Concept To Help Integrate Molecular Phylogenetics And Taxonomy, Prosanta Chakrabarty Oct 2010

Genetypes: A Concept To Help Integrate Molecular Phylogenetics And Taxonomy, Prosanta Chakrabarty

Faculty Publications

No abstract provided.

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Lsu Research Fall 2010, Louisiana State University And Agricultural And Mechanical College Oct 2010

Lsu Research Fall 2010, Louisiana State University And Agricultural And Mechanical College

Research Magazine

LSU Research is published annually by the Office of Research & Economic Development, Louisiana State University.

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Song Divergence By Sensory Drive In Amazonian Birds, Joseph A. Tobias, Job Aben, Robb T. Brumfield, Elizabeth P. Derryberry, Wouter Halfwerk, Hans Slabbekoorn, Nathalie Seddon Oct 2010

Song Divergence By Sensory Drive In Amazonian Birds, Joseph A. Tobias, Job Aben, Robb T. Brumfield, Elizabeth P. Derryberry, Wouter Halfwerk, Hans Slabbekoorn, Nathalie Seddon

Faculty Publications

Visual signals are shaped by variation in the signaling environment through a process termed sensory drive, sometimes leading to speciation. However, the evidence for sensory drive in acoustic signals is restricted to comparisons between highly dissimilar habitats, or single-species studies in which it is difficult to rule out the influence of undetected ecological variables, pleiotropic effects, or chance. Here we assess whether this form of sensory drive-often termed " acoustic adaptation" -can generate signal divergence across ecological gradients. By studying avian communities in two Amazonian forest types, we show that songs of 17 " bamboo-specialist" bird species differ in predictable …

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Evaluation Of Cryoprotectant And Cooling Rate For Sperm Cryopreservation In The Euryhaline Fish Medaka Oryzias Latipes, Huiping Yang, Michelle Norris, Richard Winn, Terrence R. Tiersch Oct 2010

Evaluation Of Cryoprotectant And Cooling Rate For Sperm Cryopreservation In The Euryhaline Fish Medaka Oryzias Latipes, Huiping Yang, Michelle Norris, Richard Winn, Terrence R. Tiersch

Faculty Publications

Medaka Oryzias latipes is a well-recognized biomedical fish model because of advantageous features such as small body size, transparency of embryos, and established techniques for gene knockout and modification. The goal of this study was to evaluate two critical factors, cryoprotectant and cooling rate, for sperm cryopreservation in 0.25-ml French straws. The objectives were to: (1) evaluate the acute toxicity of methanol, 2-methoxyethanol (ME), dimethyl sulfoxide (Me2SO), N,N-dimethylacetamide (DMA), N,N-dimethyl formamide (DMF), and glycerol with concentrations of 5%, 10%, and 15% for 60min of incubation at 4°C; (2) evaluate cooling rates from 5 to 25°C/min for freezing and their interaction …

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Distribution Of Isoflavones And Antioxidant Activities Of Soybean Cotyledon, Coat And Germ, Xiaohua Yue, Ahmed Moursy Abdallah, Zhimin Xu Oct 2010

Distribution Of Isoflavones And Antioxidant Activities Of Soybean Cotyledon, Coat And Germ, Xiaohua Yue, Ahmed Moursy Abdallah, Zhimin Xu

Faculty Publications

Soy isoflavones in the cotyledon, coat and germ of four soybean varieties from the southern U.S.A. were determined and compared. Isoflavones with aglycone, daidzein, genistein, glycitein and aglycone, withβ-glucoside, genistin, daidzin and glycitin, were found in the four varieties. The range of total soy isoflavones in cotyledon, coat and germ extracts from the varieties was 2.73-9.71, 5.56-16.94 and 27.76-81.43 mg/g, respectively. Soy germ was the richest source of isoflavones among the three soybean parts. Distribution of the isoflavones in soy cotyledon was different from that in soy coat or germ. Daidzin and glycitin were the major isoflavones in the germ …

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Plankton Community Respiration And Productivity For The Louisiana Offshore Ecosystem, July 2007 Results, Brandon Michael Boyd Oct 2010

Plankton Community Respiration And Productivity For The Louisiana Offshore Ecosystem, July 2007 Results, Brandon Michael Boyd

Honors Capstones

No abstract provided.

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Heads Or Tails: L1 Insertion-Associated 5' Homopolymeric Sequences, Thomas J. Meyer, Deepa Srikanta, Erin M. Conlin, Mark A. Batzer Sep 2010

Heads Or Tails: L1 Insertion-Associated 5' Homopolymeric Sequences, Thomas J. Meyer, Deepa Srikanta, Erin M. Conlin, Mark A. Batzer

Faculty Publications

Background. L1s are one of the most successful autonomous mobile elements in primate genomes. These elements comprise as much as 17% of primate genomes with the majority of insertions occurring via target primed reverse transcription (TPRT). Twin priming, a variant of TPRT, can result in unusual DNA sequence architecture. These insertions appear to be inverted, truncated L1s flanked by target site duplications. Results. We report on loci with sequence architecture consistent with variants of the twin priming mechanism and introduce dual priming, a mechanism that could generate similar sequence characteristics. These insertions take the form of truncated L1s with hallmarks …

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Three-Dimensional Structure Of The Weakly Associated Protein Homodimer Ser13 Using Rdcs And Paramagnetic Surface Mapping, Hsiau Wei Lee, Greg Wylie, Sonal Bansal, Xu Wang, Adam W. Barb, Megan A. Macnaughtan, Asli Ertekin, Gaetano T. Montelione, James H. Prestegard Sep 2010

Three-Dimensional Structure Of The Weakly Associated Protein Homodimer Ser13 Using Rdcs And Paramagnetic Surface Mapping, Hsiau Wei Lee, Greg Wylie, Sonal Bansal, Xu Wang, Adam W. Barb, Megan A. Macnaughtan, Asli Ertekin, Gaetano T. Montelione, James H. Prestegard

Faculty Publications

The traditional NMR-based method for determining oligomeric protein structure usually involves distinguishing and assigning intra- and intersubunit NOEs. This task becomes challenging when determining symmetric homo-dimer structures because NOE cross-peaks from a given pair of protons occur at the same position whether intra- or intersubunit in origin. While there are isotope-filtering strategies for distinguishing intra from intermolecular NOE interactions in these cases, they are laborious and often prove ineffectual in cases of weak dimers, where observation of intermolecular NOEs is rare. Here, we present an efficient procedure for weak dimer structure determination based on residual dipolar couplings (RDCs), chemical shift …

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A Global Census Of Fission Yeast Deubiquitinating Enzyme Localization And Interaction Networks Reveals Distinct Compartmentalization Profiles And Overlapping Functions In Endocytosis And Polarity, Ilektra Kouranti, Janel R. Mclean, Anna Feoktistova, Ping Liang, Alyssa E. Johnson, Rachel H. Roberts-Galbraith, Kathleen L. Gould Sep 2010

A Global Census Of Fission Yeast Deubiquitinating Enzyme Localization And Interaction Networks Reveals Distinct Compartmentalization Profiles And Overlapping Functions In Endocytosis And Polarity, Ilektra Kouranti, Janel R. Mclean, Anna Feoktistova, Ping Liang, Alyssa E. Johnson, Rachel H. Roberts-Galbraith, Kathleen L. Gould

Faculty Publications

Ubiquitination and deubiquitination are reciprocal processes that tune protein stability, function, and/or localization. The removal of ubiquitin and remodeling of ubiquitin chains is catalyzed by deubiquitinating enzymes (DUBs), which are cysteine proteases or metalloproteases. Although ubiquitination has been extensively studied for decades, the complexity of cellular roles for deubiquitinating enzymes has only recently been explored, and there are still several gaps in our understanding of when, where, and how these enzymes function to modulate the fate of polypeptides. To address these questions we performed a systematic analysis of the 20 Schizosaccharomyces pombe DUBs using confocal microscopy, proteomics, and enzymatic activity …

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The Herpes Simplex Virus Type 1 Ul20 Protein And The Amino Terminus Of Glycoprotein K (Gk) Physically Interact With Gb, Vladimir N. Chouljenko, Arun V. Iyer, Sona Chowdhury, Joohyun Kim, Konstantin G. Kousoulas Sep 2010

The Herpes Simplex Virus Type 1 Ul20 Protein And The Amino Terminus Of Glycoprotein K (Gk) Physically Interact With Gb, Vladimir N. Chouljenko, Arun V. Iyer, Sona Chowdhury, Joohyun Kim, Konstantin G. Kousoulas

Faculty Publications

Herpes simplex virus type 1 (HSV-1) glycoprotein K (gK) and the UL20 protein (UL20p) are strictly required for virus-induced cell fusion, and mutations within either the gK or UL20 gene cause extensive cell fusion (syncytium formation). We have shown that gK forms a functional protein complex with UL20p, which is required for all gK and UL20p-associated functions in the HSV-1 life cycle. Recently, we showed that the amino-terminal 82 amino acids (aa) of gK (gKa) were required for the expression of the syncytial phenotype of the mutant virus gBΔ28 lacking the carboxyl-terminal 28 amino acids of gB (V. N. Chouljenko, …

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