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Articles 6901 - 6930 of 13619
Full-Text Articles in Entire DC Network
A Herpes Simplex Virus 1 (Mckrae) Mutant Lacking The Glycoprotein K Gene Is Unable To Infect Via Neuronal Axons And Egress From Neuronal Cell Bodies, Andrew T. David, Ahmad Saied, Anu Charles, Ramesh Subramanian, Vladimir N. Chouljenko, Konstantin G. Kousoulas
A Herpes Simplex Virus 1 (Mckrae) Mutant Lacking The Glycoprotein K Gene Is Unable To Infect Via Neuronal Axons And Egress From Neuronal Cell Bodies, Andrew T. David, Ahmad Saied, Anu Charles, Ramesh Subramanian, Vladimir N. Chouljenko, Konstantin G. Kousoulas
Faculty Publications
We have shown that the herpes simplex virus 1 (HSV-1) gK gene is essential for efficient replication and spread in the corneal epithelium and trigeminal ganglion neuroinvasion in mice (A. T. David, A. Baghian, T. P. Foster, V. N. Chouljenko, and K. G. Kousoulas, Curr. Eye Res. 33:455-467, 2008). To further investigate the role of gK in neuronal infection, we utilized a microfluidic chamber system separating neuronal cell bodies and axonal termini. HSV-1 (McKrae) engineered virus constitutively expressing enhanced green fluorescence protein (GFP) was efficiently transmitted in both a retrograde and an anterograde manner. These results were corroborated by expression …
Perspectives In Ornithology: Inferring The Origins Of Lowland Neotropical Birds, Robb T. Brumfield
Perspectives In Ornithology: Inferring The Origins Of Lowland Neotropical Birds, Robb T. Brumfield
Faculty Publications
No abstract provided.
Next-Generation Sequencing Reveals Phylogeographic Structure And A Species Tree For Recent Bird Divergences, John E. Mccormack, James M. Maley, Sarah M. Hird, Elizabeth P. Derryberry, Gary R. Graves, Robb T. Brumfield
Next-Generation Sequencing Reveals Phylogeographic Structure And A Species Tree For Recent Bird Divergences, John E. Mccormack, James M. Maley, Sarah M. Hird, Elizabeth P. Derryberry, Gary R. Graves, Robb T. Brumfield
Faculty Publications
Next generation sequencing (NGS) technologies are revolutionizing many biological disciplines but have been slow to take root in phylogeography. This is partly due to the difficulty of using NGS to sequence orthologous DNA fragments for many individuals at low cost. We explore cases of recent divergence in four phylogenetically diverse avian systems using a method for quick and cost-effective generation of primary DNA sequence data using pyrosequencing. NGS data were processed using an analytical pipeline that reduces many reads into two called alleles per locus per individual. Using single nucleotide polymorphisms (SNPs) mined from the loci, we detected population differentiation …
Gaba Is Excitatory In Adult Vasopressinergic Neuroendocrine Cells, Juhee Haam, Ion R. Popescu, Linda A. Morton, Katalin C. Halmos, Ryoichi Teruyama, Yoichi Ueta, Jeffrey G. Tasker
Gaba Is Excitatory In Adult Vasopressinergic Neuroendocrine Cells, Juhee Haam, Ion R. Popescu, Linda A. Morton, Katalin C. Halmos, Ryoichi Teruyama, Yoichi Ueta, Jeffrey G. Tasker
Faculty Publications
Neuronal excitability in the adult brain is controlled by a balance between synaptic excitation and inhibition mediated by glutamate and GABA, respectively. While generally inhibitory in the adult brain, GABAA receptor activation is excitatory under certain conditions in which the GABA reversal potential is shifted positive due to intracellular Cl-accumulation, such as during early postnatal development and brain injury. However, the conditions under which GABA is excitatory are generally either transitory or pathological. Here, we reveal GABAergic synaptic inputs to be uniformly excitatory in vasopressin (VP)-secreting magnocellular neurons in the adult hypothalamus under normal conditions. The GABA reversal potential (EGABA) …
Identification Of An Oxygenic Reaction Center Psbadc Operon In The Cyanobacterium Gloeobacter Violaceus Pcc 7421, Tuan A. Nguyen, Jasmina Brescic, David J. Vinyard, Thenappan Chandrasekar, G. Charles Dismukes
Identification Of An Oxygenic Reaction Center Psbadc Operon In The Cyanobacterium Gloeobacter Violaceus Pcc 7421, Tuan A. Nguyen, Jasmina Brescic, David J. Vinyard, Thenappan Chandrasekar, G. Charles Dismukes
Faculty Publications
Gloeobacter violaceus, the earliest diverging oxyphotobacterium (cyanobacterium) on the 16S ribosomal RNA tree, has five copies of the photosystem II psbA gene encoding the D1 reaction center protein subunit. These copies are widely distributed throughout the 4.6 Mbp genome with only one copy colocalizing with other PSII subunits, in marked contrast to all other psbA genes in all publicly available sequenced genomes. A clustering of two other psb genes around psbA3 (glr2322) is unique to Gloeobacter. We provide experimental proof for the transcription of a psbA3DC operon, encoding three of the five reaction center core subunits (D1, D2, and CP43). …
Subordinate Male Cichlids Retain Reproductive Competence During Social Suppression, Jacqueline M. Kustan, Karen P. Maruska, Russell D. Fernald
Subordinate Male Cichlids Retain Reproductive Competence During Social Suppression, Jacqueline M. Kustan, Karen P. Maruska, Russell D. Fernald
Faculty Publications
Subordinate males, which are excluded from reproduction often save energy by reducing their investment in sperm production. However, if their position in a dominance hierarchy changes suddenly they should also rapidly attain fertilization capability. Here, we asked how social suppression and ascension to dominance influences sperm quality, spermatogenesis and reproductive competence in the cichlid Astatotilapia burtoni, where reproduction is tightly coupled to social status. Dominant territorial (T) males are reproductively active while subordinate non-territorial (NT) males are suppressed, but given the opportunity, NT males will perform dominance behaviours within minutes and attain T male testes size within days. Using the …
The Phylogenetic Distribution And Ecological Role Of Carbon Monoxide Oxidation In The Genus Burkholderia, Carolyn F. Weber, Gary M. King
The Phylogenetic Distribution And Ecological Role Of Carbon Monoxide Oxidation In The Genus Burkholderia, Carolyn F. Weber, Gary M. King
Faculty Publications
Burkholderia is a physiologically and ecologically diverse genus that occurs commonly in assemblages of soil and rhizosphere bacteria. Although Burkholderia is known for its heterotrophic versatility, we demonstrate that 14 distinct environmental isolates oxidized carbon monoxide (CO) and possessed the gene encoding the catalytic subunit of form I CO dehydrogenase (coxL). DNA from a Burkholderia isolate obtained from a passalid beetle also contained coxL as do the genomic sequences of species H160 and Ch1-1. Isolates were able to consume CO at concentrations ranging from 100 ppm (vol/vol) to sub-ambient (< 60 ppb (vol/vol)). High concentrations of pyruvate inhibited CO uptake (> 2.5 mM), but mixotrophic consumption of CO and pyruvate occurred …
Limited Potential For Adaptation To Climate Change In A Broadly Distributed Marine Crustacean, Morgan W. Kelly, Eric Sanford, Richard K. Grosberg
Limited Potential For Adaptation To Climate Change In A Broadly Distributed Marine Crustacean, Morgan W. Kelly, Eric Sanford, Richard K. Grosberg
Faculty Publications
The extent to which acclimation and genetic adaptation might buffer natural populations against climate change is largely unknown. Most models predicting biological responses to environmental change assume that species' climatic envelopes are homogeneous both in space and time. Although recent discussions have questioned this assumption, few empirical studies have characterized intraspecific patterns of genetic variation in traits directly related to environmental tolerance limits. We test the extent of such variation in the broadly distributed tidepool copepod Tigriopus californicus using laboratory rearing and selection experiments to quantify thermal tolerance and scope for adaptation in eight populations spanning more than 178 of …
Recruitment And Biological Consequences Of Histone Modification Of H3k27me3 And H3k9me3., Joomyeong Kim, Hana Kim
Recruitment And Biological Consequences Of Histone Modification Of H3k27me3 And H3k9me3., Joomyeong Kim, Hana Kim
Faculty Publications
Two histone marks, H3K27me3 and H3K9me3, are well known for their repressive roles in the genic and nongenic regions of metazoan genomes. Several protein complexes are known to be responsible for generating these marks, including polycomb repression complex 2 and several H3K9 methylases. Recent studies have shown that the targeting of these histone-modifying complexes within mammalian genomes may be mediated through several DNA-binding proteins, including AEBP2, JARID2, and YY1. In this review, we discuss the potential targeting mechanisms in light of the recent results that have been derived from genome-wide chromatin immunoprecipitation sequencing data and the in vivo functions of …
Components, Volume 8 (2012), Lsu Center For Computation And Technology
Components, Volume 8 (2012), Lsu Center For Computation And Technology
Components
No abstract provided.
2012 Annual Report, Louisiana Rice Research Board, Lsu Agcenter
2012 Annual Report, Louisiana Rice Research Board, Lsu Agcenter
Louisiana Rice Research Board Annual Report
No abstract provided.
Drop Deposition On Canopy And Chemical Control Of Phakopsora Pachyrhizi In Soybeans, Nédio R. Tormen, Felipe D.L. Da Silva, Mônica P. Debortoli, Juliano D. Uebel, Diego D. Fávera, Ricardo S. Balardin
Drop Deposition On Canopy And Chemical Control Of Phakopsora Pachyrhizi In Soybeans, Nédio R. Tormen, Felipe D.L. Da Silva, Mônica P. Debortoli, Juliano D. Uebel, Diego D. Fávera, Ricardo S. Balardin
Faculty Publications
Drop deposition on soybean canopy with three spray nozzles and chemical control programs of asian soybean rust were evaluated in two soybean cultivars, in a study carried out in Itaara, RS. The treatments were arranged under completely randomized block design with four replications, in a factorial design 2 × 3 × 3, utilizing two soybean cultivars, three spray nozzles and three chemical control programs. The fungicide applications were accomplished in R1 and R4 growth stages. Drop density, volumetric medium diameter (VMD), leaf area index (LAI), disease severity and grain yield were the variables measured. Based on the severity data of …
Variants In Cxadr And F2rl1 Are Associated With Blood Pressure And Obesity In African-Americans In Regions Identified Through Admixture Mapping, Priya B. Shetty, Hua Tang, Bamidele O. Tayo, Alanna C. Morrison, Craig L. Hanis, Dabeeru C. Rao, Jeffery H. Young, Ervin R. Fox, Eric Boerwinkle, Richard S. Cooper, Neil J. Risch, Xiaofeng Zhu
Variants In Cxadr And F2rl1 Are Associated With Blood Pressure And Obesity In African-Americans In Regions Identified Through Admixture Mapping, Priya B. Shetty, Hua Tang, Bamidele O. Tayo, Alanna C. Morrison, Craig L. Hanis, Dabeeru C. Rao, Jeffery H. Young, Ervin R. Fox, Eric Boerwinkle, Richard S. Cooper, Neil J. Risch, Xiaofeng Zhu
Faculty Publications
OBJECTIVE: Genetic variants in 296 genes in regions identified through admixture mapping of hypertension, BMI, and lipids were assessed for association with hypertension, blood pressure (BP), BMI, and high-density lipoprotein cholesterol (HDL-C). METHODS: This study identified coding SNPs identified from HapMap2 data that were located in genes on chromosomes 5, 6, 8, and 21, wherein ancestry association evidence for hypertension, BMI, or HDL-C was identified in previous admixture mapping studies. Genotyping was performed in 1733 unrelated African-Americans from the National Heart, Lung and Blood Institute's Family Blood Pressure Project, and gene-based association analyses were conducted for hypertension, SBP, DBP, BMI, …
Oyun: A New, Free Program For Iterated Prisoner’S Dilemma Tournaments In The Classroom, Charles H. Pence, Lara Buchak
Oyun: A New, Free Program For Iterated Prisoner’S Dilemma Tournaments In The Classroom, Charles H. Pence, Lara Buchak
Faculty Publications
Evolutionary applications of game theory present one of the most pedagogically accessible varieties of genuine, contemporary theoretical biology. We present here Oyun (oy-oon, http://charlespence.net/oyun), a program designed to run iterated prisoner's dilemma tournaments, competitions between prisoner's dilemma strategies developed by the students themselves. Using this software, students are able to readily design and tweak their own strategies, and to see how they fare both in round-robin tournaments and in “evolutionary” tournaments, where the scores in a given “generation” directly determine contribution to the population in the next generation. Oyun is freely available, runs on Windows, Mac, and Linux computers, …
Gumbo Yearbook, Class Of 2012, Louisiana State University And Agricultural And Mechanical College
Gumbo Yearbook, Class Of 2012, Louisiana State University And Agricultural And Mechanical College
Gumbo Yearbook
The Gumbo yearbook chronicles the entire academic year at LSU. In words and especially photos, the Gumbo shows the people, places, and events that make each year unique. In addition to formal portraits of schools and departments, the book contains hundreds of snapshots of students with their friends and dozens of pages showcasing organizations.
The Extrinsic Proteins Of Photosystem Ii, Terry M. Bricker, Johnna L. Roose, Robert D. Fagerlund, Laurie K. Frankel, Julian J. Eaton-Rye
The Extrinsic Proteins Of Photosystem Ii, Terry M. Bricker, Johnna L. Roose, Robert D. Fagerlund, Laurie K. Frankel, Julian J. Eaton-Rye
Faculty Publications
In this review we examine the structure and function of the extrinsic proteins of Photosystem II. These proteins include PsbO, present in all oxygenic organisms, the PsbP and PsbQ proteins, which are found in higher plants and eukaryotic algae, and the PsbU, PsbV, CyanoQ, and CyanoP proteins, which are found in the cyanobacteria. These proteins serve to optimize oxygen evolution at physiological calcium and chloride concentrations. They also shield the Mn 4CaO 5 cluster from exogenous reductants. Numerous biochemical, genetic and structural studies have been used to probe the structure and function of these proteins within the photosystem. We will …
Research Matters Summer 2012, Louisiana State University And Agricultural & Mechanical College
Research Matters Summer 2012, Louisiana State University And Agricultural & Mechanical College
Research Matters & RNR Newsletters
Research Matters is a publication which highlights ongoing research, international travel experiences, classroom happenings and degree program developments of and for the School of Renewable Natural Resources' students and faculty.
School Of Renewable Natural Resources Newsletter, Fall 2012, Louisiana State University And Agricultural & Mechanical College
School Of Renewable Natural Resources Newsletter, Fall 2012, Louisiana State University And Agricultural & Mechanical College
Research Matters & RNR Newsletters
The annual newsletter from the School of Renewable Natural Resources was a publication which aimed to update and entertain interested readers with the news and research from RNR faculty, students and staff.
Female, French, And Alone The Case Of Luisa De Dufressi Before The Mexican Inquisition During The Time Of Viceroy Bernardo De Galvez (Hero Of The Battle Of Baton Rouge And Other Notable Feats), Frieda Koeninger
1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era
No abstract provided.
Pedro De Peralta And The Catholic Enlightenment Heaven To The Heavens And Back, Renee Gutierrez
Pedro De Peralta And The Catholic Enlightenment Heaven To The Heavens And Back, Renee Gutierrez
1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era
No abstract provided.
Workshop And Panel Discussion: High-Throughput Cryopreservation Of Germplasm As An Exchange Currency For Genetic Resources, Zoltán M. Varga, Terrence R. Tiersch
Workshop And Panel Discussion: High-Throughput Cryopreservation Of Germplasm As An Exchange Currency For Genetic Resources, Zoltán M. Varga, Terrence R. Tiersch
Faculty Publications
No abstract provided.
Offspring Production With Cryopreserved Sperm From A Live-Bearing Fish Xiphophorus Maculatus And Implications For Female Fecundity, Huiping Yang, Markita G. Savage, Leona Hazlewood, Ronald B. Walter, Terrence R. Tiersch
Offspring Production With Cryopreserved Sperm From A Live-Bearing Fish Xiphophorus Maculatus And Implications For Female Fecundity, Huiping Yang, Markita G. Savage, Leona Hazlewood, Ronald B. Walter, Terrence R. Tiersch
Faculty Publications
Xiphophorus fishes are well-established models for biomedical research of spontaneous or induced tumors, and their use in research dates back to the 1930s. Currently, 58 well-pedigreed lines exist among 24 Xiphophorus species housed as live animals at the Xiphophorus Genetic Stock Center. The technique of sperm cryopreservation has been applied to preserve these valuable genetic resources, and production of offspring has been reported with cryopreserved sperm in two species (X. helleri and X. couchianus). The goal of this research was to establish protocols for sperm cryopreservation and artificial insemination that yield live young in X. maculatus, a widely used research …
Arbuscular Mycorrhizae Occur In Common Spartina Species, Angela K. Burcham, Joy H. Merino, Thomas C. Michot, John A. Nyman
Arbuscular Mycorrhizae Occur In Common Spartina Species, Angela K. Burcham, Joy H. Merino, Thomas C. Michot, John A. Nyman
Faculty Publications
We report the presence of arbuscular mycorrhizae (AM) in Spartina alterniflora Loisel roots. Roots were sampled for AM in field-collected and greenhouse-maintained Spartina patens (Aiton) Muhl. and S. alterniflora, the dominant species of Louisiana's brackish and saline marshes, respectively. Previous reports of AM association in these Spartina sp. are limited and conflicting. Field-collected 5. alterniflora had minimal AM (2.4%), whereas 39.5% of the 5. patens roots examined were AM colonized. Greenhouse conditions of reduced salinity [3 parts per thousand (ppt)] appeared to increase AM association for 5. patens compared with field samples. AM occurrence varied significantly among the three sample …
Characterization Of Lateral Root Development At The Onset Of Storage Root Initiation In 'Beauregard' Sweetpotato Adventitious Roots, Arthur Villordon, Don Labonte, Julio Solis, Nurit Firon
Characterization Of Lateral Root Development At The Onset Of Storage Root Initiation In 'Beauregard' Sweetpotato Adventitious Roots, Arthur Villordon, Don Labonte, Julio Solis, Nurit Firon
Faculty Publications
This study characterized lateral root (LR) development attributes during the onset of storage root (SR) initiation stage in 'Beauregard' sweetpotato. SR initiation has been defined as the appearance of cambia around the protoxylem and secondary xylem elements. Our results showed that 20-day-old adventitious roots (ARs) classified as SRs had 53% and 85% greater mean LR count than pencil roots (PRs) and lignified roots (LGs), respectively. SRs had 53% and 78% greater mean LR density relative to PRs and LGs, respectively. SRs had 66% and 130% greater mean total LR length than PRs and LGs, respectively. SRs had lower mean main …
Host Interferon-Γ Inducible Protein Contributes To Brucella Survival., Jennifer A. Ritchie, Adam Rupper, James A. Cardelli, Bryan H. Bellaire
Host Interferon-Γ Inducible Protein Contributes To Brucella Survival., Jennifer A. Ritchie, Adam Rupper, James A. Cardelli, Bryan H. Bellaire
Faculty Publications
Brucella spp. are highly adapted intracellular pathogens of mammals that cause chronic infections while surving and replicating in host monocytes and macrophages. Although monocytes are normally susceptible to infection, pretreatment with pro-inflammatory cytokine interferon-γ (IFN-γ) activates cellular defense mechanisms that increase intracellular killing of Brucella and prevents bacterial replication. We examined the contribution of the IFN-γ inducible GTPase, LRG-47, to B. abortus 2308 infection in in vitro and in vivo murine models. Infecting non-activated macrophages from LRG-47(-/-) mice revealed that loss of this host protein negatively effected the intracellular survival and replication of IgG opsonized B. abortus. In contrast, survival …
Overexpression Of Arabidopsis And Rice Stress Genes' Inducible Transcription Factor Confers Drought And Salinity Tolerance To Rice, Karabi Datta, Niranjan Baisakh, Moumita Ganguly, Sellapan Krishnan, Kazuko Yamaguchi Shinozaki, Swapan K. Datta
Overexpression Of Arabidopsis And Rice Stress Genes' Inducible Transcription Factor Confers Drought And Salinity Tolerance To Rice, Karabi Datta, Niranjan Baisakh, Moumita Ganguly, Sellapan Krishnan, Kazuko Yamaguchi Shinozaki, Swapan K. Datta
Faculty Publications
Rice yield is greatly affected by environmental stresses such as drought and salinity. In response to the challenge of producing rice plants tolerant to these stresses, we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter. Two different indica rice cultivars were used, BR29, an improved commercially cultivated variety from Bangladesh and IR68899B, an IRRI bred maintainer line for hybrid rice. Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice, whereas biolistic transformation was done with rice- DREB1B in the …
The Origin And In Situ Identification Of Uncultured Gut Bacteria In Fourth-Instar Larvae Of The Red Imported Fire Ant, Solenopsis Invicta (Hymenoptera: Formicidae), Albert H. Lee, Linda Hooper-Bui
The Origin And In Situ Identification Of Uncultured Gut Bacteria In Fourth-Instar Larvae Of The Red Imported Fire Ant, Solenopsis Invicta (Hymenoptera: Formicidae), Albert H. Lee, Linda Hooper-Bui
Faculty Publications
This study corroborated the findings of Lee et al. (2008) and relied on culture-independent techniques, particularly 16SrDNA sequencing. Results from the study demonstrated that ants in captivity completely change their gut bacterial diversity strongly supports the conclusion that fire ant gut bacteria are environmental in origin. Bacteria in the fourth-instar larvae were present because they were consumed with food sources. From molecular data, a differing list of bacteria from each colony with different branching and clustering among bacterial clades in the phylogenetic trees were found. Different bacterial diversity and evenness was found in all colonies, which provided evidence against the …
Biofiltration For Treatment Of Gases Contaminanted By Beta-Caryophyllene, Weili Hu
Biofiltration For Treatment Of Gases Contaminanted By Beta-Caryophyllene, Weili Hu
LSU Master's Theses
Fixed-film biological treatment processes, commonly referred to as biofilters, have been applied to successfully treat a wide variety of volatile organic compounds (VOCs) present in air emitted by a wide variety of industrial operations. The ability of biofilters to treat some classes of VOCs, however, has not been well established. In particular, the performance of biofilters treating sesquiterpenes has not been widely studied. In the research described herein, a laboratory-scale biofilter was operated to treat a synthetic waste gas stream containing â-caryophyllene, a sesquiterpene emitted by conifer trees and industrial wood processing operations. An enrichment culture developed in an initial …
Pah Concentrations Found Within Gulf Menhaden (Brevoortia Patronus) Populations Located Off Of The South/Southeast Coast Of Louisiana, Gregory Michael Olson
Pah Concentrations Found Within Gulf Menhaden (Brevoortia Patronus) Populations Located Off Of The South/Southeast Coast Of Louisiana, Gregory Michael Olson
LSU Master's Theses
In April 2010, large quantities of crude oil were released into the Gulf of Mexico (GoM) raising questions about the possible contamination of marine organisms with constituents of concern known as Polycyclic Aromatic Hydrocarbons (PAHs). In order to determine the impact of the Deepwater Horizon (DWH) crude oil spill, Gulf menhaden (Brevoortia patronus) were harvested from two coastal regions of Louisiana. Tissue analysis to determine total PAH concentration was conducted using an adapted matrix solid phase dispersion (MSPD) method; a Soxhlet extraction process was used to determine fish oil to mass ratios for “small” (fork length < 16 cm) and “large” (fork length > 16 cm) menhaden. Menhaden …
Genomic Expression Response To Experimentally-Weathered South Louisiana Crude Oil In Gulf Killifish Profiled Across Tissues, Doses And Time., Whitney Rose Pilcher
Genomic Expression Response To Experimentally-Weathered South Louisiana Crude Oil In Gulf Killifish Profiled Across Tissues, Doses And Time., Whitney Rose Pilcher
LSU Master's Theses
Almost five million barrels of south Louisiana crude oil were released into the environment following the explosion of the Deepwater Horizon oil platform on April 20, 2010, however, little is known of the effects of the spill to native species of the northern Gulf of Mexico. Field studies provide a context for ecological realism but laboratory- based studies can connect the specific cause to a specific biological response. A previous field study, completed in 2010, tracked genome expression responses in native killifish resident in oiled and several non-oiled marshes in the northern Gulf of Mexico. Those data suggested significant biological …