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Can Parasitized Lambs Self-Medicate Through Preferred Consumption Of Sericea Lespedeza Pellets?, Mallory Alicia Garcia Apr 2016

Can Parasitized Lambs Self-Medicate Through Preferred Consumption Of Sericea Lespedeza Pellets?, Mallory Alicia Garcia

Honors Capstones

No abstract provided.


Analysis Of Western Lowland Gorilla (Gorilla Gorilla Gorilla) Specific Alu Repeats, Thomas Beckstrom Apr 2016

Analysis Of Western Lowland Gorilla (Gorilla Gorilla Gorilla) Specific Alu Repeats, Thomas Beckstrom

Honors Capstones

No abstract provided.


Characterization Of Ammonium Salt Toxicity In Cabbage Family Plants, Madison Boudreaux Apr 2016

Characterization Of Ammonium Salt Toxicity In Cabbage Family Plants, Madison Boudreaux

Honors Capstones

No abstract provided.


Development Of Mouse Melanoma Model To Evaluate Oncolytic Viral Therapy Using Osv, Kelli A. Clemons Apr 2016

Development Of Mouse Melanoma Model To Evaluate Oncolytic Viral Therapy Using Osv, Kelli A. Clemons

Honors Capstones

No abstract provided.


Repellent Effect Of Formic Acid Against The Red Imported Fire Ant (Hymenoptera: Formicidae): A Field Study, Cai Wang, Gregg Henderson Apr 2016

Repellent Effect Of Formic Acid Against The Red Imported Fire Ant (Hymenoptera: Formicidae): A Field Study, Cai Wang, Gregg Henderson

Faculty Publications

Previous studies showed that the formic acid secreted by tawny crazy ants not only has fumigation toxicity to the red imported fire ant, Solenopsis invicta Buren (Chen et al. 2013), but also can detoxify fire ant venom (LeBrun et al. 2014). These lead us to a field study to determine if low concentrations of formic acid might be useful in repelling S. invicta. Filter paper discs treated with 1.3% or 5% formic acid (v: v) or distilled water (control) were placed on each of the 46 S. invicta mounds and a disturbance was created. For a minute or less, there …


Frequency Of Cry1f Non-Recessive Resistance Alleles In North Carolina Field Populations Of Spodoptera Frugiperda (Lepidoptera: Noctuidae), Guoping Li, Dominic Reisig, Jin Miao, Fred Gould, Fangneng Huang, Hongqiang Feng Apr 2016

Frequency Of Cry1f Non-Recessive Resistance Alleles In North Carolina Field Populations Of Spodoptera Frugiperda (Lepidoptera: Noctuidae), Guoping Li, Dominic Reisig, Jin Miao, Fred Gould, Fangneng Huang, Hongqiang Feng

Faculty Publications

Fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a target species of transgenic corn (Zea mays L.) that expresses single and pyramided Bacillus thuringiensis (Bt) toxin. In 2014, S. frugiperda were collected from a light trap in North Carolina, and a total of 212 F1/F2 isofemale lines of S. frugiperda were screened for resistance to Bt and non-Bt corn. All of the 212 isolines were susceptible to corn tissue expressing Cry1A.105 + Cry2Ab, Cry1F + Cry1A.105 + Cry2Ab, and Cry1F + Cry1Ab + Vip3Aa20. Growth rate bioassays were performed to isolate non-recessive Bt resistance alleles. Seven individuals out of …


Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer Apr 2016

Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer

Honors Capstones

No abstract provided.


Determination Of Sperm Concentration Using Flow Cytometry With Simultaneous Analysis Of Sperm Plasma Membrane Integrity In Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Terrence R. Tiersch Apr 2016

Determination Of Sperm Concentration Using Flow Cytometry With Simultaneous Analysis Of Sperm Plasma Membrane Integrity In Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Terrence R. Tiersch

Faculty Publications

Control of sperm concentration is required to ensure consistent and reproducible results for cryopreservation and in vitro fertilization protocols. Determination of sperm concentration is traditionally performed with a counting chamber (e.g., hemocytometer), or more recently with a spectrophotometer. For small-sized biomedical model fishes, the availability of sperm sample is limited to microliters, so it is desirable to develop fast and accurate approaches for concentration determination that also minimize sample use. In this study, a new approach was developed for sperm concentration determination using a flow cytometer (Accuri C6, BD Biosciences, San Jose, CA) with simultaneous measurement of sperm membrane integrity …


A Procedure-Spanning Analysis Of Plasma Membrane Integrity For Assessment Of Cell Viability In Sperm Cryopreservation Of Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Carrie Carmichael, Jen Matthews, Zoltan M. Varga, Terrence Tiersch Apr 2016

A Procedure-Spanning Analysis Of Plasma Membrane Integrity For Assessment Of Cell Viability In Sperm Cryopreservation Of Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Carrie Carmichael, Jen Matthews, Zoltan M. Varga, Terrence Tiersch

Faculty Publications

The goal of this study was to evaluate plasma membrane integrity and motility for zebrafish sperm quality assessment along the cryopreservation pathway - from sample collection through refrigerated storage, cryoprotectant equilibration, freezing, thawing, and fertilization. The objectives were to: (1) evaluate the effects of osmolality, extender, and refrigerated storage on sperm plasma membrane integrity and motility, and (2) compare cryopreservation of sperm from farm-raised and well-characterized research populations by evaluating motility and membrane integrity of fresh, post-equilibration (before freezing) and post-thaw sperm, and post-thaw fertility. Osmolality, extender, and storage time each influenced sperm motility and membrane integrity. Isotonic osmolality showed …


Response Of Plant Height, Species Richness And Aboveground Biomass To Flooding Gradient Along Vegetation Zones In Floodplain Wetlands, Northeast China, Yanjing Lou, Yanwen Pan, Chuanyu Gao, Ming Jiang, Xianguo Lu, Y. Jun Xu Apr 2016

Response Of Plant Height, Species Richness And Aboveground Biomass To Flooding Gradient Along Vegetation Zones In Floodplain Wetlands, Northeast China, Yanjing Lou, Yanwen Pan, Chuanyu Gao, Ming Jiang, Xianguo Lu, Y. Jun Xu

Faculty Publications

Flooding regime changes resulting from natural and human activity have been projected to affect wetland plant community structures and functions. It is therefore important to conduct investigations across a range of flooding gradients to assess the impact of flooding depth on wetland vegetation. We conducted this study to identify the pattern of plant height, species richness and aboveground biomass variation along the flooding gradient in floodplain wetlands located in Northeast China. We found that the response of dominant species height to the flooding gradient depends on specific species, i.e., a quadratic response for Carex lasiocarpa, a negative correlation for Calamagrostis …


Climate, Habitat Associations And The Potential Distributions Of Neotropical Birds: Implications For Diversification Across The Andes, Carlos Daniel Cadena, Carlos A. Pedraza, Robb T. Brumfield Apr 2016

Climate, Habitat Associations And The Potential Distributions Of Neotropical Birds: Implications For Diversification Across The Andes, Carlos Daniel Cadena, Carlos A. Pedraza, Robb T. Brumfield

Faculty Publications

Contrary to the long-held view that the uplift of the Andes spurred biotic diversification by causing vicariance across multiple lineages with formerly continuous distributions, recent work suggests that dispersal across the Andes occurring after major uplift episodes was a major driver of speciation in Neotropical birds, with the ability of lineages to persist and disperse in the landscape being strong predictors of speciation. However, where and when dispersal events across the Andes occurred is unknown, and the role of climatic fluctuations and associated shifts in vegetation in promoting cross-Andes dispersal at different moments remains to be studied. We used models …


Comparisons And Correlations Of Phenolic Profiles And Anti-Oxidant Activities Of Seventeen Varieties Of Pineapple, Liqing Du, Guangming Sun, Xiumei Zhang, Yuge Liu, Witoon Prinyawiwatkul, Zhimin Xu, Yixiao Shen Apr 2016

Comparisons And Correlations Of Phenolic Profiles And Anti-Oxidant Activities Of Seventeen Varieties Of Pineapple, Liqing Du, Guangming Sun, Xiumei Zhang, Yuge Liu, Witoon Prinyawiwatkul, Zhimin Xu, Yixiao Shen

Faculty Publications

Major phenolic, β-carotene, and ascorbic acid (AA) contents in 17 pineapple varieties were quantified and compared. Anti-oxidant activities were evaluated using 2,2-Diphenyl-l-picrylhydrazyl (DPPH), 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing anti-oxidant power (FRAP), and metal chelating capacity (MCC) assays. MD-2 exhibited the highest AA and total phenolic (TP) contents and DPPH and ABTS assay results, but was lower in β-carotene contents. Ripley had the highest total flavonoid (TF) content with a low AA content. Comte de Pairs exhibited the highest MCC and the lowest FRAP values. TP contents and both DPPH and ABTS activities, FRAP values and both AA contents and DPPH …


The Potential Role Of Polyamines In Gill Epithelial Remodeling During Extreme Hypoosmotic Challenges In The Gulf Killifish, Fundulus Grandis, Ying Guan, Guo Xia Zhang, Shujun Zhang, Beau Domangue, Fernando Galvez Apr 2016

The Potential Role Of Polyamines In Gill Epithelial Remodeling During Extreme Hypoosmotic Challenges In The Gulf Killifish, Fundulus Grandis, Ying Guan, Guo Xia Zhang, Shujun Zhang, Beau Domangue, Fernando Galvez

Faculty Publications

© 2016 Elsevier Inc. Polyamines are a family of low molecular weight organic cations produced in part by the coordinated actions of arginase II (Arg II) and ornithine decarboxylase (Odc). Although gill polyamine homeostasis is affected by acute transfer to fresh water, little is known of its function in fish osmoregulation. The current study investigated the role of polyamines in the compensatory response of hypoosmotic challenge in the euryhaline fish, Fundulus grandis. Adult F. grandis were acclimated to 5ppt water, transferred abruptly to 5, 2, 1, 0.5 and 0.1 ppt water, and assessed for osmoregulatory function, gill morphology, and polyamine …


Environmental Stress In The Gulf Of Mexico And Its Potential Impact On Public Health, B. Singleton, J. Turner, L. Walter, N. Lathan, D. Thorpe, P. Ogbevoen, J. Daye, D. Alcorn, S. Wilson, J. Semien, T. Richard, T. Johnson, K. Mccabe, J. J. Estrada, F. Galvez, C. Velasco, K. Reiss Apr 2016

Environmental Stress In The Gulf Of Mexico And Its Potential Impact On Public Health, B. Singleton, J. Turner, L. Walter, N. Lathan, D. Thorpe, P. Ogbevoen, J. Daye, D. Alcorn, S. Wilson, J. Semien, T. Richard, T. Johnson, K. Mccabe, J. J. Estrada, F. Galvez, C. Velasco, K. Reiss

Faculty Publications

© 2015 Elsevier Inc. The Deepwater Horizon (DWH) oil spill in the Gulf of Mexico was the largest maritime oil spill in history resulting in the accumulation of genotoxic substances in the air, soil, and water. This has potential far-reaching health impacts on cleanup field workers and on the populations living in the contaminated coastal areas. We have employed portable airborne particulate matter samplers (SKC Biosampler Impinger) and a genetically engineered bacterial reporter system (umu-ChromoTest from EBPI) to determine levels of genotoxicity of air samples collected from highly contaminated areas of coastal Louisiana including Grand Isle, Port Fourchon, and Elmer's …


Road Associated Wildlife Mortality In An Urbanizing Metropolitan Area Of The Southeastern Usa, Lillian Miller Apr 2016

Road Associated Wildlife Mortality In An Urbanizing Metropolitan Area Of The Southeastern Usa, Lillian Miller

Honors Capstones

No abstract provided.


Zein Nanoparticle Interaction With Soybean Plants, Kurt Ristroph Apr 2016

Zein Nanoparticle Interaction With Soybean Plants, Kurt Ristroph

Honors Capstones

No abstract provided.


Understanding Inequality In Special Education: A State Level Analysis Of High School Special Education Graduation Rates, Haley Paulish Apr 2016

Understanding Inequality In Special Education: A State Level Analysis Of High School Special Education Graduation Rates, Haley Paulish

Honors Capstones

No abstract provided.


“Ain’T He A Beauty”: Black Queer Aesthetics And Disidentification In American Literature And Film, Rory Tolbert Boothe Apr 2016

“Ain’T He A Beauty”: Black Queer Aesthetics And Disidentification In American Literature And Film, Rory Tolbert Boothe

Honors Capstones

No abstract provided.


Single Cell Encapsulation Using A Microfluidic Droplet Generator, Seleipiri Charles Apr 2016

Single Cell Encapsulation Using A Microfluidic Droplet Generator, Seleipiri Charles

Honors Capstones

No abstract provided.


15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler Apr 2016

15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler

Honors Capstones

No abstract provided.


Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker Apr 2016

Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker

Honors Capstones

No abstract provided.


Women Do It Better: The Inclusion Of Women In Post-Conflict Peace Negotiations, Mary Scott Wofford Apr 2016

Women Do It Better: The Inclusion Of Women In Post-Conflict Peace Negotiations, Mary Scott Wofford

Honors Capstones

No abstract provided.


Characteristic Length Scales Of The Secondary Relaxations In Glass-Forming Glycerol, Sudipta Gupta, Eugene Mamontov, Niina Jalarvo, Laura Stingaciu, Michael Ohl Mar 2016

Characteristic Length Scales Of The Secondary Relaxations In Glass-Forming Glycerol, Sudipta Gupta, Eugene Mamontov, Niina Jalarvo, Laura Stingaciu, Michael Ohl

Faculty Publications

We investigate the secondary relaxations and their link to the main structural relaxation in glass-forming liquids using glycerol as a model system. We analyze the incoherent neutron scattering signal dependence on the scattering momentum transfer, Q , in order to obtain the characteristic length scale for different secondary relaxations. Such a capability of neutron scattering makes it somewhat unique and highly complementary to the traditional techniques of glass physics, such as light scattering and broadband dielectric spectroscopy, which provide information on the time scale, but not the length scales, of relaxation processes. The choice of suitable neutron scattering techniques depends …


Discovery Of Novel Nonactive Site Inhibitors Of The Prothrombinase Enzyme Complex, Karan Kapoor, Nicole Mcgill, Cynthia B. Peterson, Harold V. Meyers, Michael N. Blackburn, Jerome Baudry Mar 2016

Discovery Of Novel Nonactive Site Inhibitors Of The Prothrombinase Enzyme Complex, Karan Kapoor, Nicole Mcgill, Cynthia B. Peterson, Harold V. Meyers, Michael N. Blackburn, Jerome Baudry

Faculty Publications

© 2016 American Chemical Society. The risk of serious bleeding is a major liability of anticoagulant drugs that are active-site competitive inhibitors targeting the Factor Xa (FXa) prothrombin (PT) binding site. The present work identifies several new classes of small molecule anticoagulants that can act as nonactive site inhibitors of the prothrombinase (PTase) complex composed of FXa and Factor Va (FVa). These new classes of anticoagulants were identified, using a novel agnostic computational approach to identify previously unrecognized binding pockets at the FXa-FVa interface. From about three million docking calculations of 281 128 compounds in a conformational ensemble of FXa …


Astrocytes Regulate Glp-1 Receptor-Mediated Effects On Energy Balance, David J. Reiner, Elizabeth G. Mietlicki-Baase, Lauren E. Mcgrath, Derek J. Zimmer, Kendra K. Bence, Gregory L. Sousa, Vaibhav R. Konanur, Joanna Krawczyk, David H. Burk, Scott E. Kanoski, Gerlinda E. Hermann, Richard C. Rogers, Matthew R. Hayes Mar 2016

Astrocytes Regulate Glp-1 Receptor-Mediated Effects On Energy Balance, David J. Reiner, Elizabeth G. Mietlicki-Baase, Lauren E. Mcgrath, Derek J. Zimmer, Kendra K. Bence, Gregory L. Sousa, Vaibhav R. Konanur, Joanna Krawczyk, David H. Burk, Scott E. Kanoski, Gerlinda E. Hermann, Richard C. Rogers, Matthew R. Hayes

Faculty Publications

© 2016 the authors. Astrocytes are well established modulators of extracellular glutamate, but their direct influence on energy balance-relevant behaviors is largely understudied. As the anorectic effects of glucagon-like peptide-1 receptor (GLP-1R) agonists are partly mediated by central modulation of glutamatergic signaling, we tested the hypothesis that astrocytic GLP-1R signaling regulates energy balance in rats. Central or peripheral administration of a fluorophore-labeled GLP-1R agonist, exendin-4, localizes within astrocytes and neurons in the nucleus tractus solitarius (NTS), a hindbrain nucleus critical for energy balance control. This effect is mediated by GLP-1R, as the uptake of systemically administered fluorophore-tagged exendin-4 was blocked …


Pelvic Sexual Dimorphism Among Species Monomorphic In Body Size: Relationship To Relative Newborn Body Mass, Robert G. Tague Mar 2016

Pelvic Sexual Dimorphism Among Species Monomorphic In Body Size: Relationship To Relative Newborn Body Mass, Robert G. Tague

Faculty Publications

Females have larger pelves than males among eutherians to mitigate obstetrical difficulty. This study addresses 3 issues concerning pelvic sexual dimorphism using 8 species that are sexually monomorphic in nonpelvic size: Aotus azarae, Castor canadensis, Dasypus novemcinctus, Hylobates lar, Saguinus geoffroyi, Sciurus carolinensis, Sylvilagus floridanus, and Urocyon cinereoargenteus. Using published data to compute the index of relative newborn body mass (RNBM = [newborn body mass/adult female body mass]100%) for 266 eutherian species, A. azarae, H. lar, and S. geoffroyi are characterized as giving birth to relatively large newborns and the other 5 species as giving birth to relatively small newborns. …


Synthesis And In Vitro Studies Of A Series Of Carborane-Containing Boron Dipyrromethenes (Bodipys), Sunting Xuan, Ning Zhao, Zehua Zhou, Frank R. Fronczek, M. Graça H. Vicente Mar 2016

Synthesis And In Vitro Studies Of A Series Of Carborane-Containing Boron Dipyrromethenes (Bodipys), Sunting Xuan, Ning Zhao, Zehua Zhou, Frank R. Fronczek, M. Graça H. Vicente

Faculty Publications

© 2016 American Chemical Society. A series of seven BODIPYs functionalized with ortho-carborane groups at the 8(meso) or 3/5(α) position were synthesized and characterized by NMR, HRMS, HPLC, and in the cases of 2b and 5b, by X-ray analysis. The BODIPYs exhibited low dark toxicity and phototoxicity toward human glioma T98G cells, and their cellular uptake varied significantly, with 5b accumulating the most and 7 the least. All BODIPYs localized mainly within the cell ER. The BODIPYs showed higher permeabilities than lucifer yellow across human hCMEC/D3 brain endothelial cell monolayers as the BBB model. Among this series, 1b showed the …


Amidine-Mediated Zwitterionic Ring-Opening Polymerization Of N-Alkyl N-Carboxyanhydride: Mechanism, Kinetics, And Architecture Elucidation, Ang Li, Lu Lu, Xin Li, Li Lin He, Changwoo Do, Jayne C. Garno, Donghui Zhang Mar 2016

Amidine-Mediated Zwitterionic Ring-Opening Polymerization Of N-Alkyl N-Carboxyanhydride: Mechanism, Kinetics, And Architecture Elucidation, Ang Li, Lu Lu, Xin Li, Li Lin He, Changwoo Do, Jayne C. Garno, Donghui Zhang

Faculty Publications

© 2016 American Chemical Society. Zwitterionic ring-opening polymerization (ZROP) of N-butyl N-carboxyanhydrides (Bu-NCAs) has been investigated using 1,8-diazabicycloundec-7-ene (DBU), a bicyclic amidine initiator. It was found that poly(N-butylglycine)s (PNBGs) with molecular weight (Mn) in the 3.5-32.4 kg mol-1 range and polydispersity index (PDI) in the 1.02-1.12 range can be readily obtained by systematically varying the initial monomer to initiator feed ratio. The polymerization exhibits characteristics of a controlled polymerization, as evidenced by the linear increase of polymer molecular weight with conversion and the successful enchainment experiments. Kinetic studies revealed that the reaction is first-order dependent on the monomer and the …


Dietary Plant Lectins Appear To Be Transported From The Gut To Gain Access To And Alter Dopaminergic Neurons Of Caenorhabditis Elegans, A Potential Etiology Of Parkinson’S Disease, Jolene Zheng, Mingming Wang, Wenqian Wei, Jeffrey N. Keller, Binita Adhikari, Jason F. King, Michael L. King, Nan Peng, Roger A. Laine Mar 2016

Dietary Plant Lectins Appear To Be Transported From The Gut To Gain Access To And Alter Dopaminergic Neurons Of Caenorhabditis Elegans, A Potential Etiology Of Parkinson’S Disease, Jolene Zheng, Mingming Wang, Wenqian Wei, Jeffrey N. Keller, Binita Adhikari, Jason F. King, Michael L. King, Nan Peng, Roger A. Laine

Faculty Publications

© Copyright © 2016 Zheng, Wang, Wei, Keller, Adhikari, King, King, Peng and Laine. Lectins from dietary plants have been shown to enhance drug absorption in the gastrointestinal tract of rats, be transported trans-synaptically as shown by tracing of axonal and dendritic paths, and enhance gene delivery. Other carbohydrate-binding protein toxins are known to traverse the gut intact in dogs. Post-feeding rhodamine- or TRITC-tagged dietary lectins, the lectins were tracked from gut to dopaminergic neurons (DAergic-N) in transgenic Caenorhabditis elegans (C. elegans) [egIs1(Pdat-1:GFP)] where the mutant has the green fluorescent protein (GFP) gene fused to a dopamine transport protein gene …


Attosecond Transient Absorption In Dense Gases: Exploring The Interplay Between Resonant Pulse Propagation And Laser-Induced Line-Shape Control, Chen Ting Liao, Arvinder Sandhu, Seth Camp, Kenneth J. Schafer, Mette B. Gaarde Mar 2016

Attosecond Transient Absorption In Dense Gases: Exploring The Interplay Between Resonant Pulse Propagation And Laser-Induced Line-Shape Control, Chen Ting Liao, Arvinder Sandhu, Seth Camp, Kenneth J. Schafer, Mette B. Gaarde

Faculty Publications

We investigate the evolution of extreme ultraviolet (XUV) spectral line shapes in an optically thick helium gas under near-infrared (IR) perturbation. In our experimental and theoretical work, we systematically vary the IR intensity, time-delay, gas density, and IR polarization parameters to study line-shape modifications induced by collective interactions in a regime beyond the single-atom response of a thin, dilute gas. In both experiment and theory, we find that specific features in the frequency-domain absorption profile, and their evolution with propagation distance, can be attributed to the interplay between resonant attosecond pulse propagation and IR-induced phase shifts. Our calculations show that …