Digital Commons Network^™

The Role Of Sediment-Induced Light Attenuation On Primary Production During Hurricane Gustav (2008), Zhengchen Zang, Z. George Xue, Kehui Xu, Samuel J. Bentley, Qin Chen, Eurico J. D'Sa, Le Zhang, Yanda Ou

Faculty Publications

© Author(s) 2020. This work is distributed under the Creative Commons Attribution 4.0 License. We introduced a sediment-induced light attenuation algorithm into a biogeochemical model of the Coupled Ocean-Atmosphere-Wave-Sediment Transport (COAWST) modeling system. A fully coupled ocean-atmospheric- sediment-biogeochemical simulation was carried out to assess the impact of sediment-induced light attenuation on primary production in the northern Gulf of Mexico during the passage of Hurricane Gustav in 2008. When compared with model results without sediment-induced light attenuation, our new model showed a better agreement with satellite data on both the magnitude of nearshore chlorophyll concentration and the spatial distribution of offshore …

Prdm16 Deficiency Leads To Age-Dependent Cardiac Hypertrophy, Adverse Remodeling, Mitochondrial Dysfunction, And Heart Failure, Dasan Mary Cibi, Kathleen Wung Bi-Lin, Shamini Guna Shekeran, Reddemma Sandireddy, Nicole Tee, Anamika Singh, Yajun Wu, Dinesh Kumar Srinivasan, Jean Paul Kovalik, Sujoy Ghosh, Patrick Seale, Manvendra K. Singh

Division of Basic Science Faculty Publications

Hypertrophic cardiomyopathy (HCM) is a well-established risk factor for cardiovascular mortality worldwide. Although hypertrophy is traditionally regarded as an adaptive response to physiological or pathological stress, prolonged hypertrophy can lead to heart failure. Here we demonstrate that Prdm16 is dispensable for cardiac development. However, it is required in the adult heart to preserve mitochondrial function and inhibit hypertrophy with advanced age. Cardiac-specific deletion of Prdm16 results in cardiac hypertrophy, excessive ventricular fibrosis, mitochondrial dysfunction, and impaired metabolic flexibility, leading to heart failure. We demonstrate that Prdm16 and euchromatic histone-lysine N-methyltransferase factors (Ehmts) act together to reduce expression of fetal genes …

Novel Information About The Kinetic Effects Of Equine Shoe Modifications And Kinematic Effects Of Human Digital Devices For Improved Performance In Both Species, Pengju Wang

LSU Master's Theses

Equine shoes are frequently modified to enhance traction for horses that travel on paved surfaces for work, pleasure, or entertainment. Little is known about other common shoe modifications used to enhance traction like calks, tungsten carbide granules, or plastic composition. This information is vital to shoe design to protect the safety and welfare of all service, working, and leisure horses. The objective of the first part of this thesis was to quantify the effect of shoes with and without traction adaptions on kinetic measures in non-lame, light breed horses at a trot. Kinetic data was collected with a force platform …

Comparison Of High-Tech Augmentative And Alternative Communication Interfaces: Do Age And Technology Experience Matter?, Surani Gopika Nakkawita

LSU Doctoral Dissertations

Previous research has demonstrated that individuals with stroke-induced aphasia can use augmentative and alternative communication (AAC) when they cannot meet their communication needs using spoken language (Dietz, Weissling, Griffith, McKelvey, & Macke, 2014; Purdy & Van Dyke, 2011). Of the various interfaces found in the different AAC systems, the grid display and the visual scene display (VSD) have been used by individuals with aphasia (Hough & Johnson, 2009; Dietz et al., 2018). However, there is a scarcity of research examining the comparative usefulness of these interfaces.

This prospective study attempted to understand how neurologically healthy individuals of different ages and …

Life And Death In The Chicxulub Impact Crater: A Record Of The Paleocene-Eocene Thermal Maximum, Vann Smith, Sophie Warny, Kliti Grice, Bettina Schaefer, Michael T. Whalen, Johan Vellekoop, Elise Chenot, Sean P.S. Gulick, Ignacio Arenillas, Jose A. Arz, Thorsten Bauersachs, Timothy Bralower, Francois Demory, Jerome Gattacceca, Heather Jones, Johanna Lofi, Christopher M. Lowery, Joanna Morgan, Noelia B. Nuñez Otaño, Jennifer M.K. O'Keefe, Katherine O'Malley, Francisco J. Rodríguez-Tovar, Lorenz Schwark

Faculty Publications

© 2020 Royal Society of Chemistry. All rights reserved. Thermal stress on the biosphere during the extreme warmth of the Paleocene-Eocene Thermal Maximum (PETM) was most severe at low latitudes, with sea surface temperatures at some localities exceeding the 35 C at which marine organisms experience heat stress. Relatively few equivalent terrestrial sections have been identified, and the response of land plants to this extreme heat is still poorly understood. Here, we present a new record of the PETM from the peak ring of the Chicxulub impact crater that has been identified based on nannofossil biostratigraphy, an acme of the …

Bruce Gates: A Career In Catalysis, Cawas Cooper, Kerry M. Dooley, Juan C. Fierro-Gonzalez, Javier Guzman, Rolf Jentoft, H. Henry Lamb, Isao Ogino, Ron C. Runnebaum, Ajit Sapre, Alper Uzun

Faculty Publications

On the occasion of his 80th birthday, we take stock of the remarkable and ongoing career of Professor Bruce C. Gates. This Account highlights his key scientific achievements in three areas of significance: supported metal clusters and atomically dispersed metal complexes, hydroprocessing reaction networks and catalysts, and strong acid catalysis. His contributions in all three areas are noteworthy and have been sustained over 50 years. The work of his group evolved from mainly catalyst synthesis and reaction studies to also incorporate sophisticated characterization techniques based on X-ray absorption spectroscopies and improved scanning transmission electron microscopic examination of catalytic surfaces. In …

Distribution Of Aromatase In The Brain Of The African Cichlid Fish Astatotilapia Burtoni: Aromatase Expression, But Not Estrogen Receptors, Varies With Female Reproductive-State, Karen P. Maruska, Julie M. Butler, Chase Anselmo, Ganga Tandukar

Faculty Publications

© 2020 Wiley Periodicals, Inc. Estrogen synthesis and signaling in the brains of vertebrates has pleotropic effects ranging from neurogenesis to modulation of behaviors. The majority of studies on brain-derived estrogens focus on males, but estrogenic signaling in females likely plays important roles in regulation of reproductive cycling and social behaviors. We used females of the mouth brooding African cichlid fish, Astatotilapia burtoni, to test for reproductive state-dependent changes in estrogenic signaling capacity within microdissected brain nuclei that are important for social behaviors. Expression levels of the rate-limiting enzyme aromatase, but not estrogen receptors, measured by qPCR changes across the …

Neural Activation Patterns Associated With Maternal Mouthbrooding And Energetic State In An African Cichlid Fish, Karen P. Maruska, Julie M. Butler, Karen E. Field, Christopher Forester, Ashley Augustus

Faculty Publications

© 2020 IBRO Parental care is widespread in the animal kingdom, but for many species, provisioning energetic resources must be balanced with trade-offs between self-promoting and offspring-promoting behaviors. However, little is known about the neural mechanisms underlying these motivational decisions. Mouthbrooding is an extreme form of parental care most common in fishes that provides an ideal opportunity to examine which brain regions are involved in parenting and energetics. The African cichlid fish Astatotilapia burtoni is a maternal mouthbrooder in which females hold developing young inside their mouths for 2 weeks. This brood care makes feeding impossible, so females undergo obligatory …

Identification Of A Novel Endornavirus In Geranium Carolinianum And Occurrence Within Three Agroecosystems, Rachel Herschlag, Ryo Okada, Ricardo Iván Alcalá-Briseño, Eliezer Rodrigues De Souto, Rodrigo A. Valverde

Faculty Publications

A putative endornavirus was detected in Carolina geranium (Geranium carolinianum) in Louisiana, USA. The virus was provisionally named Geranium carolinianum endornavirus 1 (GcEV1). The viral RNA was sequenced, and it consisted of 14,625 nt containing a single ORF coding a putative polyprotein of 4815 aa with conserved domains for a helicase 1, peptidase C97, glycosyl transferase GTB-type, and RNA-dependent RNA polymerase 2. The 5′end consisted of 130 nt while the 3′end consisted of 54 nt ending in nine cytosine residues. The closest relative to GcEV1 was Phaseolus vulgaris endornavirus 3. In phylogenetic analyses, GcEV1 clustered with members of the genus …

Tunable Dual Plasmon-Induced Transparency Based On A Monolayer Graphene Metamaterial And Its Terahertz Sensing Performance, Jiahao Ge, Chenglong You, He Feng, Xiaoman Li, Mei Wang, Lifeng Dong, Georgios Veronis, Maojin Yun

Faculty Publications

In this paper, tunable dual plasmon-induced transparency (PIT) is achieved by using a monolayer graphene metamaterial in the terahertz region, which consists of two graphene strips of different sizes and a graphene ring. As the dual PIT effect is induced by the destructive interference between the two quasi-dark modes and the bright mode, we propose a four-level plasmonic system based on the linearly coupled Lorentzian oscillators to explain the mechanism behind the dual PIT. It is proved that the theoretical results agree well with the simulation results. Most importantly, the sensing properties of the designed device have been investigated in …

The Life History And Population Dynamics Of Southern Flounder, Kenneth A. Erickson

LSU Master's Theses

Southern Flounder, Paralichthys lethostigma, are a coastal, estuarine-dependent flatfish species that inhabits the Southeastern US Atlantic Ocean and Gulf of Mexico. Throughout their range, Southern Flounder are exploited by both commercial and recreational fisheries. Recently, numerous sources have expressed concerns about Southern Flounder populations, with stock assessments indicating declines in spawning stock biomass and recruitment. To estimate life history traits needed for informed management, such as age, growth, and maturity, I collected 327 Southern Flounder from Louisiana and obtained 14,184 historical records from the Louisiana Department of Wildlife and Fisheries. Using a von Bertalanffy growth equation in a Bayesian …

Electronic Self‐Reporting: Angler Attitudes And Behaviors In The Recreational Red Snapper Fishery, Stephen R. Midway, Jason Adriance, Patrick Banks, Sepp Haukebo, Rex Caffey

Faculty Publications

No abstract provided.

Ideas And Perspectives: The Same Carbon Behaves Like Different Elements - An Insight Into Position-Specific Isotope Distributions, Yuyang He, Xiaobin Cao, Huiming Bao

Faculty Publications

It is expected that information on the source, reaction pathway, and reaction kinetics of an organic compound can be obtained from its position-specific isotope compositions or intramolecular isotope distribution (Intra-ID). To retrieve the information, we could use its predicted equilibrium Intra-ID as a reference for understanding the observed Intra-IDs. Historically, observed, apparently close-to-equilibrium carbon Intra-ID has prompted an open debate on the nature of biosystems and specifically the pervasiveness of reversible biochemical reactions. Much of the debate remains unresolved, and the discussion has not clearly distinguished between two states of equilibrium: (1) the equilibrium among the corresponding bond-breaking and bond-forming …

The Dynamic Trophic Niche Of An Island Bird Of Prey, Ulises Balza, Nicolas A. Lois, Michael J. Polito, Klemens Puetz, Amira Saalom, Andrea Raya Rey

Faculty Publications

Optimal foraging theory predicts an inverse relationship between the availability of preferred prey and niche width in animals. Moreover, when individuals within a population have identical prey preferences and preferred prey is scarce, a nested pattern of trophic niche is expected if opportunistic and selective individuals can be identified. Here, we examined intraspecific variation in the trophic niche of a resident population of striated caracara (Phalcoboenus australis) on Isla de los Estados (Staten Island), Argentina, using pellet and stable isotope analyses. While this raptor specializes on seabird prey, we assessed this population's potential to forage on terrestrial prey, especially invasive …

Fish Assemblages Associated With Artificial Reefs Assessed Using Multiple Gear Types In The Northwest Gulf Of Mexico, Jeffrey D. Plumlee, Kaylan M. Dance, Michael A. Dance, Hay R. Rooker, Thomas C. Tinhan, J. Brooke Shipley, R. J. David Wells

Faculty Publications

Quantitative surveys of fishes associated with artificial reefs in the northwest Gulf of Mexico were conducted over a 4-yr period (2014-2017). Artificial reefs surveyed were comprised of three types: concrete structures, rig jackets, and decommissioned ships. All reefs were surveyed using vertical long line ( VLL), fish traps, and Adaptive Resolution Imaging Sonar (ARIS 1800). Mean fish abundance did not significantly differ using VLL [1.7 ind set(-1) (SD 2.2)] among the three reef types. However, relative abundance among all fishes collected was significantly highest on rig reefs using traps [6.2 ind soak(-1) (SD 3.8)], while results from sonar surveys indicated …

Tidal And Storm Impacts On Hydrodynamics And Sediment Dynamics In An Energetic Ebb Tidal Delta, Kehui Xu, P. Ansley Wren, Yanxia Ma

Faculty Publications

Bottom-mounted instrumentation was deployed at two sites on a large sandy shoal of an ebb tidal delta offshore of the Port Royal Sound of South Carolina of USA to collect hydrodynamics and sediment dynamics data. One site ("borrow site") was 2 km offshore in a dredge pit for nearby beach nourishment and the other site ("reference site") was 10 km offshore. In situ time-series data were collected during two periods after the dredging: 15 March-12 June (spring) and 18 August-18 November (fall) of 2012. Data at the reference site indicated active migrating bedforms from centimeters to decimeters tall, and sediment …

Backbone And Sidechain Resonance Assignments And Secondary Structure Of Scc4 From Chlamydia Trachomatis, Thilini O. Ukwaththage, Marco Tonelli, Megan A. Macnaughtan

Faculty Publications

© 2020, Springer Nature B.V. Chlamydia trachomatis is an obligate intracellular bacterium that causes the most common sexually transmitted bacterial diseases in the world. With a biphasic developmental cycle, the bacteria utilize a type III secretion system (T3SS) to invade host cells as infectious elemental bodies, which then differentiate into actively dividing reticulate bodies. The regulation of the developmental cycle and the T3SS are linked by the bi-functional protein, specific Chlamydia chaperone 4 (Scc4). Scc4 is a class I T3SS chaperone forming a heterodimer with specific Chlamydia chaperone 1 (Scc1) to chaperone the essential virulence effector, Chlamydia outer membrane protein …

Expression, Purification, And Glycosylation Of Epidermal Growth Factor-Like Repeat 27 From Mouse Notch1, Justin A. Grennell, Kendra D. Jenkins, Huimin Zhong, Amid Paudyal, Kelvin B. Luther, Robert S. Haltiwanger, Megan A. Macnaughtan

Faculty Publications

© 2020 Elsevier Inc. Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells. Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions. Each EGF repeat consists of about 40 amino acids with 3 conserved disulfide bonds. Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat. EGF repeat 27 (EGF27) from mouse NOTCH1 is …

The Central Role Of Taxonomy In The Study Of Neotropical Biodiversity1, Laura P. Lagomarsino, Laura A. Frost

Faculty Publications

© 2020 Missouri Botanical Garden. All rights reserved. The Neotropics are the most species-rich area of the planet. Understanding the origin and maintenance of this diversity is an important goal of ecology and evolutionary biology. Success in this endeavor relies heavily on the past work of taxonomists who have collected specimens and produced the floras and monographs that constitute the foundation for the study of plant diversity. To illustrate this, we visualize collecting efforts through time and identify the importance of past taxonomic and collection efforts in generating the bulk of specimen data that broad-scale analyses rely on today. To …

Abundance Of The Species Clostridium Butyricum In The Gut Microbiota Contributes To Differences In Obesity Phenotype In Outbred Sprague-Dawley Cd Rats, Diana N. Obanda, Claudia Husseneder, Anne M. Raggio, Ryan Page, Brian Marx, Rhett W. Stout, Justin Guice, Diana Coulon, Michael J. Keenan

Faculty Publications

© 2020 Elsevier Inc. Objectives: Gut microbiota profiles contribute to differences in obesity phenotype. We examined the abundance of the species Clostridium butyricum in relation to obesity phenotype. Methods: In outbred Sprague -Dawley rats we examined effects of dietary fat, resistant starch (RS), and a microbiota transplant on obesity phenotype. Using targeted qPCR, we examined the abundance of total gut bacteria and C. butyricum in relation to the propensity of obesity prone and obesity resistant rats to accumulate abdominal fat. Results: Before inclusion of dietary RS, obesity resistant (OR) rats had higher amounts of total bacteria, and C. butyricum compared …

Abundance Of The Species Clostridium Butyricum In The Gut Microbiota Contributes To Differences In Obesity Phenotype In Outbred Sprague-Dawley Cd Rats, Diana N. Obanda, Claudia Husseneder, Anne M. Raggio, Ryan Page, Brian Marx, Rhett W. Stout, Justin Guice, Diana Coulon, Michael J. Keenan

Faculty Publications

© 2020 Elsevier Inc. Objectives: Gut microbiota profiles contribute to differences in obesity phenotype. We examined the abundance of the species Clostridium butyricum in relation to obesity phenotype. Methods: In outbred Sprague -Dawley rats we examined effects of dietary fat, resistant starch (RS), and a microbiota transplant on obesity phenotype. Using targeted qPCR, we examined the abundance of total gut bacteria and C. butyricum in relation to the propensity of obesity prone and obesity resistant rats to accumulate abdominal fat. Results: Before inclusion of dietary RS, obesity resistant (OR) rats had higher amounts of total bacteria, and C. butyricum compared …

The Anti-Hiv Drug Nelfinavir Mesylate (Viracept) Is A Potent Inhibitor Of Cell Fusion Caused By The Sarscov-2 Spike (S) Glycoprotein Warranting Further Evaluation As An Antiviral Against Covid-19 Infections, Farhana Musarrat, Vladimir Chouljenko, Achyut Dahal, Rafiq Nabi, Tamara Chouljenko, Seetharama D. Jois, Konstantin G. Kousoulas

Faculty Publications

© 2020 Wiley Periodicals LLC Severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) is the causative agent of the coronavirus disease-2019 (COVID-19) pandemic. Coronaviruses enter cells via fusion of the viral envelope with the plasma membrane and/or via fusion of the viral envelope with endosomal membranes after virion endocytosis. The spike (S) glycoprotein is a major determinant of virus infectivity. Herein, we show that the transient expression of the SARS CoV-2 S glycoprotein in Vero cells caused extensive cell fusion (formation of syncytia) in comparison to limited cell fusion caused by the SARS S glycoprotein. Both S glycoproteins were detected intracellularly …

Skeletal And Muscular Pelvic Morphology Of Hillstream Loaches (Cypriniformes: Balitoridae), Callie H. Crawford, Zachary S. Randall, Pamela B. Hart, Lawrence M. Page, Prosanta Chakrabarty, Apinun Suvarnaraksha, Brooke E. Flammang

Faculty Publications

© 2020 Wiley Periodicals LLC The rheophilic hillstream loaches (Balitoridae) of South and Southeast Asia possess a range of pelvic girdle morphologies, which may be attributed to adaptations for locomotion against rapidly flowing water. Specifically, the connectivity of the pelvic plate (basipterygium) to the vertebral column via a sacral rib, and the relative size and shape of the sacral rib, fall within a spectrum of three discrete morphotypes: long, narrow rib that meets the basipterygium; thicker, slightly curved rib meeting the basipterygium; and robust crested rib interlocking with the basipterygium. Species in this third category with more robust sacral rib …

All-Atomic Molecular Dynamic Studies Of Human And Drosophila Cdk8: Insights Into Their Kinase Domains, The Lxxll Motifs, And Drug Binding Site, Wu Xu, Xiao-Jun Xie, Ali K. Faust, Mengmeng Liu, Xiao Li, Feng Chen, Ashlin A. Naquin, Avery C. Walton, Peter W. Kishbaugh, Jun-Yuan Ji

Faculty Publications

Cyclin-dependent kinase 8 (CDK8) and its regulatory partner Cyclin C (CycC) play conserved roles in modulating RNA polymerase II (Pol II)-dependent gene expression. To understand the structure and function relations of CDK8, we analyzed the structures of human and Drosophila CDK8 proteins using molecular dynamics simulations, combined with functional analyses in Drosophila. Specifically, we evaluated the structural differences between hCDK8 and dCDK8 to predict the effects of the LXXLL motif mutation (AQKAA), the P154L mutations, and drug binding on local structures of the CDK8 proteins. First, we have observed that both the LXXLL motif and the kinase activity of CDK8 …

Dietary Methionine Restriction Signals To The Brain Through Fibroblast Growth Factor 21 To Regulate Energy Balance And Remodeling Of Adipose Tissue, Laura A. Forney, Han Fang, Landon C. Sims, Kirsten P. Stone, Leighann Y. Vincik, Alicia M. Vick, Amanda N. Gibson, David H. Burk, Thomas W. Gettys

Faculty Publications

© 2020 The Obesity Society. Objective: Restricting dietary methionine to 0.17% in mice increases energy expenditure (EE), reduces fat deposition, and improves metabolic health by increasing hepatic fibroblast growth factor 21 (FGF21). The goal of this study was to compare each of these responses in mice with the coreceptor for FGF21 deleted in either adipose tissue or the brain. Methods: Methionine-restriction (MR) diets were fed to age-matched cohorts of mice with the coreceptor for FGF21 deleted in either adipose tissue or the brain. The physiological and transcriptional responses to MR were compared in the respective cohorts. Results: Tissue-specific deletion of …

Effect Of Conjugated Estrogens And Bazedoxifene On Glucose, Energy And Lipid Metabolism In Obese Postmenopausal Women, Kara L. Marlatt, Dragana Lovre, Robbie A. Beyl, Chandra R. Tate, Evelyn K. Hayes, Charles F. Burant, Eric Ravussin, Franck Mauvais-Jarvis

Clinical Research Faculty Publications

Objective: Combining conjugated estrogens (CE) with the selective estrogen receptor modulator bazedoxifene (BZA) is a novel, orally administered menopausal therapy. We investigated the effect of CE/BZA on insulin sensitivity, energy metabolism, and serum metabolome in postmenopausal women with obesity. Design: Randomized, double-blind, crossover pilot trial with washout was conducted at Pennington Biomedical Research Center. Eight postmenopausal women (age 50-60 years, BMI 30-40 kg/m2) were randomized to 8 weeks CE/BZA or placebo. Primary outcome was insulin sensitivity (hyperinsulinemic-euglycemic clamp). Secondary outcomes included body composition (DXA); resting metabolic rate (RMR); substrate oxidation (indirect calorimetry); ectopic lipids (1H-MRS); fat cell size, adipose and …

Lsu Research Fall 2020, Louisiana State University And Agricultural And Mechanical College

Research Magazine

LSU Research is published annually by the Office of Research & Economic Development, Louisiana State University.

The Case For Re-Examining Glycosylation Inhibitors, Mimetics, Primers And Glycosylation Decoys As Antivirals And Anti-Inflammatories In Covid19, Roger A. Laine

Faculty Publications

No abstract provided.

Montana Native Entomopathogenic Nematode Species Against Limonius Californicus (Coleoptera: Elateridae), Ramandeep Kaur Sandhi, David Shapiro-Ilan, Gadi V.P. Reddy

Faculty Publications

Wireworms are destructive soil inhabiting polyphagous pests in the Pacific Northwest and Intermountain region of the United States. Continuously increasing wireworm populations and damage in small grain crops such as spring wheat in Northern Great Plains has become a challenge for growers. Due to unavailability of effective control measures, alternative methods, including biological control agents such as entomopathogenic nematodes (EPNs) are needed. Native/indigenous EPN species are expected to have better potential than exotic species to control the local insect pests. Two Montana native EPN species (Steinernema feltiae and Heterorhabditis bacteriophora) were tested against, Limonius californicus (Coleoptera: Elateridae) in laboratory and …

Environmentally Clean Access To Antarctic Subglacial Aquatic Environments, Alexander B. Michaud, Trista (Vick-Majors, Amanda M. Achberger, Mark L. Skidmore, Brent C. Christner, Martyn Tranter, John C. Priscu

Faculty Publications

Subglacial Antarctic aquatic environments are important targets for scientific exploration due to the unique ecosystems they support and their sediments containing palaeoenvironmental records. Directly accessing these environments while preventing forward contamination and demonstrating that it has not been introduced is logistically challenging. The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project designed, tested and implemented a microbiologically and chemically clean method of hot-water drilling that was subsequently used to access subglacial aquatic environments. We report microbiological and biogeochemical data collected from the drilling system and underlying water columns during sub-ice explorations beneath the McMurdo and Ross ice shelves and …