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A Reference Genome For Bluegill (Centrarchidae: Lepomis Macrochirus), William B. Ludt, Eamon C. Corbett, Jerry Kattawar, Prosanta Chakrabarty, Brant C. Faircloth Mar 2023

A Reference Genome For Bluegill (Centrarchidae: Lepomis Macrochirus), William B. Ludt, Eamon C. Corbett, Jerry Kattawar, Prosanta Chakrabarty, Brant C. Faircloth

Faculty Publications

North American sunfishes (Family Centrarchidae) are among the most popular sportfish throughout the United States and Canada. Despite the popularity of sunfishes, their ecological importance, and their extensive stocking and aquacultural history, few molecular studies have examined the evolutionary relationships and species boundaries among members of this group, many of which are known to hybridize. Here, we describe a chromosome-scale genome assembly representing Bluegill (Lepomis macrochirus), one of the most widespread centrarchid species. By combining long-read, Oxford Nanopore sequencing data with short-insert, whole-genome and HiC sequence reads, we produced an assembly (Lm_LA_1.1) having a total length of 889 Mb including …

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Dna Assays For Genetic Discrimination Of Three Phragmites Australis Subspecies In The United States, Denise L. Lindsay, Xin Guan, Nathan E. Harms, James T. Cronin, Laura A. Meyerson, Richard F. Lance Mar 2023

Dna Assays For Genetic Discrimination Of Three Phragmites Australis Subspecies In The United States, Denise L. Lindsay, Xin Guan, Nathan E. Harms, James T. Cronin, Laura A. Meyerson, Richard F. Lance

Faculty Publications

Premise: To genetically discriminate subspecies of the common reed (Phragmites australis), we developed real-time quantitative (qPCR) assays for identifying P. australis subsp. americanus, P. australis subsp. australis, and P. australis subsp. berlandieri. Methods and Results: Utilizing study-generated chloroplast DNA sequences, we developed three novel qPCR assays. Assays were verified on individuals of each subspecies and against two non-target species, Arundo donax and Phalaris arundinacea. One assay amplifies only P. australis subsp. americanus, one amplifies P. australis subsp. australis and/or P. australis subsp. berlandieri, and one amplifies P. australis subsp. americanus and/or P. australis subsp. australis. This protocol enhances currently available …

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Unhealthy Herds And The Predator–Spreader: Understanding When Predation Increases Disease Incidence And Prevalence, Robert L. Richards, Bret D. Elderd, Meghan A. Duffy Mar 2023

Unhealthy Herds And The Predator–Spreader: Understanding When Predation Increases Disease Incidence And Prevalence, Robert L. Richards, Bret D. Elderd, Meghan A. Duffy

Faculty Publications

Disease ecologists now recognize the limitation behind examining host–parasite interactions in isolation: community members—especially predators—dramatically affect host–parasite dynamics. Although the initial paradigm was that predation should reduce disease in prey populations (“healthy herds hypothesis”), researchers have realized that predators sometimes increase disease in their prey. These “predator–spreaders” are now recognized as critical to disease dynamics, but empirical research on the topic remains fragmented. In a narrow sense, a “predator–spreader” would be defined as a predator that mechanically spreads parasites via feeding. However, predators affect their prey and, subsequently, disease transmission in many other ways such as altering prey population structure, …

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Pseudomonas Aeruginosa Dps (Pa0962) Functions In H2o2 Mediated Oxidative Stress Defense And Exhibits In Vitro Dna Cleaving Activity, Nimesha Rajapaksha, Anabel Soldano, Huili Yao, Fabrizio Donnarumma, Maithri M. Kashipathy, Steve Seibold, Kevin P. Battaile, Scott Lovell, Mario Rivera Mar 2023

Pseudomonas Aeruginosa Dps (Pa0962) Functions In H2o2 Mediated Oxidative Stress Defense And Exhibits In Vitro Dna Cleaving Activity, Nimesha Rajapaksha, Anabel Soldano, Huili Yao, Fabrizio Donnarumma, Maithri M. Kashipathy, Steve Seibold, Kevin P. Battaile, Scott Lovell, Mario Rivera

Faculty Publications

We report the structural, biochemical, and functional characterization of the product of gene PA0962 from Pseudomonas aeruginosa PAO1. The protein, termed Pa Dps, adopts the Dps subunit fold and oligomerizes into a nearly spherical 12-mer quaternary structure at pH 6.0 or in the presence of divalent cations at neutral pH and above. The 12-Mer Pa Dps contains two di-iron centers at the interface of each subunit dimer, coordinated by conserved His, Glu, and Asp residues. In vitro, the di-iron centers catalyze the oxidation of Fe2+ utilizing H2O2 (not O2) as an oxidant, suggesting Pa Dps functions to aid P. …

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The Interaction Of Rurality And Rare Cancers For Travel Time To Cancer Care, Tracy Onega, Jennifer Alford-Teaster, Chris Leggett, Andrew Loehrer, Julie E. Weiss, Erika L. Moen, Catherine C. Pollack, Fahui Wang Mar 2023

The Interaction Of Rurality And Rare Cancers For Travel Time To Cancer Care, Tracy Onega, Jennifer Alford-Teaster, Chris Leggett, Andrew Loehrer, Julie E. Weiss, Erika L. Moen, Catherine C. Pollack, Fahui Wang

Faculty Publications

Purpose: Geographic access to cancer care is known to significantly impact utilization and outcomes. Longer travel times have negative impacts for patients requiring highly specialized care, such as for rare cancers, and for those in rural areas. Scant population-based research informs geographic access to care for rare cancers and whether rurality impacts that access. Methods: Using Medicare data (2014-2015), we identified prevalent cancers and cancer-directed surgeries, chemotherapy, and radiation. We classified cancers as rare (incidence <6/100,000/year) or common (incidence ≥6/100,000/year) using previously published thresholds and categorized rurality from ZIP code of beneficiary residence. We estimated travel time between beneficiaries and providers for each service based on ZIP code. Descriptive statistics summarized travel time by rare versus common cancers, service type, and rurality. Findings: We included 1,169,761 Medicare beneficiaries (21.9% in nonmetropolitan areas), 87,399; 7.5% had rare cancers, with 9,133,003 cancer-directed services. Travel times for cancer services ranged from approximately 29 minutes (25th percentile) to 68 minutes (75th percentile). Travel times were similar for rare and common cancers overall (median: 45 vs 43 minutes) but differed by service type; 13.4% of surgeries were >2 hours away for rare cancers, compared to 8.3% for common cancers. Increasing rurality disproportionately increased travel time to surgical care for rare compared to …

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Surface Plasmon Resonance (Spr) Sensor For Cancer Biomarker Detection, Sreyashi Das, Ram Devireddy, Manas Ranjan Gartia Mar 2023

Surface Plasmon Resonance (Spr) Sensor For Cancer Biomarker Detection, Sreyashi Das, Ram Devireddy, Manas Ranjan Gartia

Faculty Publications

A biomarker is a physiological observable marker that acts as a stand-in and, in the best-case scenario, forecasts a clinically significant outcome. Diagnostic biomarkers are more convenient and cost-effective than directly measuring the ultimate clinical outcome. Cancer is among the most prominent global health problems and a major cause of morbidity and death globally. Therefore, cancer biomarker assays that are trustworthy, consistent, precise, and verified are desperately needed. Biomarker-based tumor detection holds a lot of promise for improving disease knowledge at the molecular scale and early detection and surveillance. In contrast to conventional approaches, surface plasmon resonance (SPR) allows for …

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Type I Diabetes Results From Immune Cell Invasion Into Pancreatic Islets, Mary Grace Beck Mar 2023

Type I Diabetes Results From Immune Cell Invasion Into Pancreatic Islets, Mary Grace Beck

Honors Capstones

No abstract provided.

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Effect Of Nadph Oxidase On The Microbial And Chemical Contamination Of Water, Grace Sheets Mar 2023

Effect Of Nadph Oxidase On The Microbial And Chemical Contamination Of Water, Grace Sheets

Honors Capstones

No abstract provided.

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Florawear: Wearable Living Interface, Hye Yeon Nam, Janiece Campbell, Andrew M. Webb, Brendan Harmon Feb 2023

Florawear: Wearable Living Interface, Hye Yeon Nam, Janiece Campbell, Andrew M. Webb, Brendan Harmon

Faculty Publications

While nature can benefit people both mentally and physically, contemporary society has become increasingly disconnected from nature. To rebuild a stronger connection with nature in our everyday life, we introduce FloraWear, a do-it-yourself, wearable living interface, that enables people to easily and closely connect with plants. This pictorial introduces how knowledge is built and shared with others using hybrid craft and fabrication, illustrates the material experiments and design development for FloraWear, and discusses how it affects wearers. Then we summarize how FloraWear can help catalyze a shift in people's perspectives towards nature. By developing emotional ties to their wearable plants, …

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Asprosin Promotes Feeding Through Sk Channel-Dependent Activation Of Agrp Neurons, Bing Feng, Hesong Liu, Ila Mishra, Clemens Duerrschmid, Peiyu Gao, Pingwen Xu, Chunmei Wang, Yanlin He Feb 2023

Asprosin Promotes Feeding Through Sk Channel-Dependent Activation Of Agrp Neurons, Bing Feng, Hesong Liu, Ila Mishra, Clemens Duerrschmid, Peiyu Gao, Pingwen Xu, Chunmei Wang, Yanlin He

Division of Basic Science Faculty Publications

Asprosin, a recently identified adipokine, activates agouti-related peptide (AgRP) neurons in the arcuate nucleus of the hypothalamus (ARH) via binding to protein tyrosine phosphatase receptor δ (Ptprd) to increase food intake. However, the intracellular mechanisms responsible for asprosin/Ptprd-mediated activation of AgRPARH neurons remain unknown. Here, we demonstrate that the small-conductance calcium-activated potassium (SK) channel is required for the stimulatory effects of asprosin/Ptprd on AgRPARH neurons. Specifically, we found that deficiency or elevation of circulating asprosin increased or decreased the SK current in AgRPARH neurons, respectively. AgRPARH-specific deletion of SK3 (an SK channel subtype highly expressed in AgRPARH neurons) blocked asprosin-induced …

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Analysis And Technical Optimization Of Processing Condition For Better Quality Of Robusta Coffee Production, Usman Ahmad, Khalimatus Sa’Diyah, Sathivel Subramaniam Feb 2023

Analysis And Technical Optimization Of Processing Condition For Better Quality Of Robusta Coffee Production, Usman Ahmad, Khalimatus Sa’Diyah, Sathivel Subramaniam

Faculty Publications

The wet processing of robusta coffee starts with harvesting coffee cherries, followed by sorting the cherries, pulping, fermentation, washing, drying, hard skin hulling, bean sorting, and packaging. Soaking fruit before pulping is an alternative for coffee farmers to delay the pulping process if time does not al-low it. The fermentation process aims to remove mucus that is firmly attached to the hard skin. The addition of L. casei bacteria can accelerate the process but affect the final taste. This study aimed to optimize the duration of soaking and fermentation processes for obtaining coffee beans with a minimum number of unpeeled …

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Enhanced Phase Sensitivity In A Mach-Zehnder Interferometer Via Photon Recycling, Dong Li, Chun Hua Yuan, Xiaoping Ma, Qingle Wang, Hwang Lee, Yao Yao, Weiping Zhang Feb 2023

Enhanced Phase Sensitivity In A Mach-Zehnder Interferometer Via Photon Recycling, Dong Li, Chun Hua Yuan, Xiaoping Ma, Qingle Wang, Hwang Lee, Yao Yao, Weiping Zhang

Faculty Publications

We propose an alternative scheme for phase estimation in a Mach-Zehnder interferometer (MZI) with photon recycling. It is demonstrated that with the same coherent-state input and homodyne detection, our proposal possesses a phase sensitivity beyond the traditional MZI. For instance, it can achieve an enhancement factor of ∼9.32 in the phase sensitivity compared with the conventional scheme even with a photon loss of 10% on the photon-recycled arm. From another point of view, the quantum Cramér-Rao bound (QCRB) is also investigated. It is found that our scheme is able to achieve a lower QCRB than the traditional one. Intriguingly, the …

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Atomistic Simulation Studies Of Thin Film Growth And Plastic Deformation In Metals And Metal/Ceramic Nanostructures, Reza Namakian Feb 2023

Atomistic Simulation Studies Of Thin Film Growth And Plastic Deformation In Metals And Metal/Ceramic Nanostructures, Reza Namakian

LSU Doctoral Dissertations

Despite the significant improvements in manufacturing and synthesis processes of metals and ceramics in the past decades, there are still areas in which the procedure is still frequently more of an art or skill rather than a science. Therefore, systematic and combined experimental and computational studies are required to facilitate the development of techniques that offer thorough understanding of the events taking place during manufacturing and synthesis processes. With regard to these issues, it is paramount to address microscale characterizations and atomic scale understanding of the events during fabrication processes. One of the focuses of this study is unraveling fundamental …

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A Comprehensive Framework For The Use Of Sugarcane Bagasse Ash As Cement And Sand Replacement In Concrete Materials, Sujata Subedi Feb 2023

A Comprehensive Framework For The Use Of Sugarcane Bagasse Ash As Cement And Sand Replacement In Concrete Materials, Sujata Subedi

LSU Doctoral Dissertations

Sugarcane bagasse ash (BA), an agricultural byproduct, has been suggested as a sustainable material to partially replace cement and sand in traditional concrete. However, the feasibility of using BA in novel concrete materials, such as engineered cementitious composites (ECC) and fiber-reinforced geopolymer composites (FRGC), has not been investigated. Therefore, the objectives of this study are to: (a) characterize the properties of Louisiana BA produced from different methodologies; (b) quantify the influence of BA as supplementary cementitious material (SCM) on concrete properties; (c) assess the feasibility of developing normal- and high-strength ECCs using BA as SCM and fine aggregate, and (d) …

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Effects Of Neighborhood Disadvantage And Peer Deviance On Conduct Problems: Interactions With Age Of Onset, Courtney Marie Goetz Feb 2023

Effects Of Neighborhood Disadvantage And Peer Deviance On Conduct Problems: Interactions With Age Of Onset, Courtney Marie Goetz

LSU Master's Theses

Youth with conduct problems (CP) generally fall into two developmental classifications, child-onset and adolescent-onset, which exhibit different causal processes and life course trajectories. Research suggests that child-onset CP is more likely to be related to individual predispositions, while adolescent-onset CP is more associated with social factors, such as peer delinquency. Living in impoverished and disorganized neighborhoods increases the risk for associating with deviant peers. Thus, the current study tested the hypothesis that neighborhood factors would be more strongly associated with adolescent-onset CP than child-onset CP, which would be explained by a greater association with deviant peers. Linear and negative binomial …

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Peat Decomposition And Erosion Contribute To Pond Deepening In A Temperate Salt Marsh, Sheron Luk, Meagan J. Eagle, Giulio Mariotti, Kelsey Gosselin, Jonathan Sanderman, Amanda C. Spivak Feb 2023

Peat Decomposition And Erosion Contribute To Pond Deepening In A Temperate Salt Marsh, Sheron Luk, Meagan J. Eagle, Giulio Mariotti, Kelsey Gosselin, Jonathan Sanderman, Amanda C. Spivak

Faculty Publications

Salt marsh ponds expand and deepen over time, potentially reducing ecosystem carbon storage and resilience. The water filled volumes of ponds represent missing carbon due to prevented soil accumulation and removal by erosion and decomposition. Removal mechanisms have different implications as eroded carbon can be redistributed while decomposition results in loss. We constrained ponding effects on carbon dynamics in a New England marsh and determined whether expansion and deepening impact nearby soils by conducting geochemical characterizations of cores from three ponds and surrounding high marshes and models of wind-driven erosion. Radioisotope profiles demonstrate that ponds are not depositional environments and …

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Assessing Impacts Of Climate Change On Selected Foundation Species And Ecosystem Services In The South-Central Usa, R. James Ansley, Victor H. Rivera-Monroy, Kerry Griffis-Kyle, Bruce Hoagland, Amanda Emert, Todd Fagin, Scott R. Loss, Heather R. Mccarthy, Nicholas G. Smith, Elizabeth F. Waring Feb 2023

Assessing Impacts Of Climate Change On Selected Foundation Species And Ecosystem Services In The South-Central Usa, R. James Ansley, Victor H. Rivera-Monroy, Kerry Griffis-Kyle, Bruce Hoagland, Amanda Emert, Todd Fagin, Scott R. Loss, Heather R. Mccarthy, Nicholas G. Smith, Elizabeth F. Waring

Faculty Publications

Climate change, interacting with and exacerbating anthropogenic modifications to the landscape, is altering ecosystem structure and function, biodiversity, and species distributions. Among the most visible short-term impacts are the altered ecological roles of foundation species—those species, native or non-native—that create locally stable environmental conditions and strongly influence ecosystem services. Understanding the future of these species is crucial for projecting impacts on ecosystem services at both local and regional scales. Here we present foundation species by ecoregion study cases across the US South-Central Region (Louisiana, New Mexico, Oklahoma, and Texas), including C4 grasses, mesquite, and northern bobwhite in the Southern Great …

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Metagenomics And Metatranscriptomics Reveal Broadly Distributed, Active, Novel Methanotrophs In The Gulf Of Mexico Hypoxic Zone And In The Marine Water Column, Kathryn L. Howe, Kiley W. Seitz, Lauren G. Campbell, Brett J. Baker, J. Cameron Thrash, Nancy N. Rabalais, Mary Kate Rogener, Samantha B. Joye, Olivia U. Mason Feb 2023

Metagenomics And Metatranscriptomics Reveal Broadly Distributed, Active, Novel Methanotrophs In The Gulf Of Mexico Hypoxic Zone And In The Marine Water Column, Kathryn L. Howe, Kiley W. Seitz, Lauren G. Campbell, Brett J. Baker, J. Cameron Thrash, Nancy N. Rabalais, Mary Kate Rogener, Samantha B. Joye, Olivia U. Mason

Faculty Publications

The northern Gulf of Mexico (nGOM) hypoxic zone is a shallow water environment where methane, a potent greenhouse gas, fluxes from sediments to bottom water and remains trapped due to summertime stratification. When the water column is destratified, an active planktonic methanotrophic community could mitigate the efflux of methane, which accumulates to high concentrations, to the atmosphere. To investigate the possibility of such a biofilter in the nGOM hypoxic zone we performed metagenome assembly, and metagenomic and metatranscriptomic read mapping. Methane monooxygenase (pmoA) was an abundant transcript, yet few canonical methanotrophs have been reported in this environment, suggesting a role …

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Secular Trends In Physical Fitness Of Peruvian Children Living At High-Altitude, Carla Santos, Sara Pereira, Olga Vasconcelos, Go Tani, Donald Hedeker, Peter T. Katzmarzyk, José Maia, Alcibíades Bustamante Feb 2023

Secular Trends In Physical Fitness Of Peruvian Children Living At High-Altitude, Carla Santos, Sara Pereira, Olga Vasconcelos, Go Tani, Donald Hedeker, Peter T. Katzmarzyk, José Maia, Alcibíades Bustamante

Population and Public Health Science Faculty Publications

The physical fitness of children is an important marker of health, and monitoring its temporal changes provides important information for developing interventions. We aimed to: (1) describe secular trends in physical fitness across age, within each sex, in Peruvian schoolchildren; and (2) verify if these trends are also present when adjusted for changes in height and weight. We sampled 1590 children (707 in 2009; 883 in 2019), aged 6–11 years. Physical fitness was assessed with four tests from the EUROFIT battery. ANOVA and ANCOVA statistical models were used. Results showed that with increasing age, girls and boys were significantly stronger …

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Influence Of Soldiers On Exploratory Foraging Behavior In The Formosan Subterranean Termite, Coptotermes Formosanus (Blattodea: Rhinotermitidae), Joseph Mccarthy, Arjun Khadka, Hasim Hakanoglu, Qian Sun Feb 2023

Influence Of Soldiers On Exploratory Foraging Behavior In The Formosan Subterranean Termite, Coptotermes Formosanus (Blattodea: Rhinotermitidae), Joseph Mccarthy, Arjun Khadka, Hasim Hakanoglu, Qian Sun

Faculty Publications

Termites are eusocial insects that live in organized colonies consisting of reproductives, workers, and soldiers. Soldiers are specialized for defense but are expensive to maintain, as they are incapable of husbandry and must be fed and groomed by workers. The soldiers of several species influence foraging behavior by acting as scouts that initiate foraging or by mediating worker behavioral plasticity during food exploration. These behaviors imply that soldiers may play a keystone role in termite colony function, apart from defense. Subterranean termite workers tunnel through soil in search of food while accompanied by varying proportions of soldiers, depending on the …

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Genetic Characterization Of The Partial Disease Resistance Of Rice To Bacterial Panicle Blight And Sheath Blight By Combined Qtl Linkage And Qtl-Seq Analyses, John Christian Ontoy, Bishnu Shrestha, Hari Sharan Karki, Inderjit Barphagha, Brijesh Angira, Adam Famoso, Jong Hyun Ham Feb 2023

Genetic Characterization Of The Partial Disease Resistance Of Rice To Bacterial Panicle Blight And Sheath Blight By Combined Qtl Linkage And Qtl-Seq Analyses, John Christian Ontoy, Bishnu Shrestha, Hari Sharan Karki, Inderjit Barphagha, Brijesh Angira, Adam Famoso, Jong Hyun Ham

Faculty Publications

Bacterial panicle blight (BPB) and sheath blight (SB) are major diseases of rice and few cultivars have shown a high level of resistance to these diseases. A recombinant inbred line (RIL) population developed from the U.S. cultivars Jupiter (moderately resistant) and Trenasse (susceptible) was investigated to identify loci associated with the partial disease resistance to BPB and SB. Disease phenotypes in BPB and SB, as well as the days-to-heading (DTH) trait, were evaluated in the field. DTH was correlated to BPB and SB diseases, while BPB was positively correlated to SB in the field trials with this RIL population. Genotyping …

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Wheat Long Noncoding Rnas From Organelle And Nuclear Genomes Carry Conserved Microrna Precursors Which May Together Comprise Intricate Networks In Insect Responses, Bala Ani Akpinar, Tugdem Muslu, Gadi V.P. Reddy, Munevver Dogramaci, Hikmet Budak Feb 2023

Wheat Long Noncoding Rnas From Organelle And Nuclear Genomes Carry Conserved Microrna Precursors Which May Together Comprise Intricate Networks In Insect Responses, Bala Ani Akpinar, Tugdem Muslu, Gadi V.P. Reddy, Munevver Dogramaci, Hikmet Budak

Faculty Publications

Long noncoding RNAs (lncRNAs) are a diverse class of noncoding RNAs that are typically longer than 200 nucleotides but lack coding potentials. Advances in deep sequencing technologies enabled a better exploration of this type of noncoding transcripts. The poor sequence conservation, however, complicates the identification and annotation of lncRNAs at a large scale. Wheat is among the leading food staples worldwide whose production is threatened by both biotic and abiotic stressors. Here, we identified putative lncRNAs from durum wheat varieties that differ in stem solidness, a major source of defense against wheat stem sawfly, a devastating insect pest. We also …

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Novel Index To Predict Mortality And Inform Malnutrition?, Jacob T. Mey Feb 2023

Novel Index To Predict Mortality And Inform Malnutrition?, Jacob T. Mey

Division of Basic Science Faculty Publications

No abstract provided.

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Wetland Occupancy By Duck Broods In Cropland-Dominated Landscapes Of The United States Prairie Pothole Region, Blake J. Mitchell, Catrina V. Terry, Kevin M. Ringelman, Kaylan M. Kemink, Michael J. Anteau, Adam K. Janke Feb 2023

Wetland Occupancy By Duck Broods In Cropland-Dominated Landscapes Of The United States Prairie Pothole Region, Blake J. Mitchell, Catrina V. Terry, Kevin M. Ringelman, Kaylan M. Kemink, Michael J. Anteau, Adam K. Janke

Faculty Publications

The Prairie Pothole Region (PPR) is globally important for breeding waterfowl but has been altered via wetland drainage and grassland conversion to accommodate agricultural land use. Thus, understanding the ecology of waterfowl in these highly modified landscapes is essential for their conservation. Brood occurrence is the cumulative outcome of key life-history events including pair formation and territory establishment, nest success, and early brood survival. We applied new technological advances in brood surveying methods to understand brood use of wetlands and how land use and wetland-specific factors influenced brood use of 413 wetlands in crop-dominated landscapes in the PPR of Iowa, …

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Glucagon-Like Peptide-1/Glucagon Receptor Agonism Associates With Reduced Metabolic Adaptation And Higher Fat Oxidation: A Randomized Trial, Karen D. Corbin, Elvis A. Carnero, Timothy D. Allerton, Joachim Tillner, Christopher P. Bock, Pierre Philippe Luyet, Britta Göbel, Kevin D. Hall, Stephanie A. Parsons, Eric Ravussin, Steven R. Smith Feb 2023

Glucagon-Like Peptide-1/Glucagon Receptor Agonism Associates With Reduced Metabolic Adaptation And Higher Fat Oxidation: A Randomized Trial, Karen D. Corbin, Elvis A. Carnero, Timothy D. Allerton, Joachim Tillner, Christopher P. Bock, Pierre Philippe Luyet, Britta Göbel, Kevin D. Hall, Stephanie A. Parsons, Eric Ravussin, Steven R. Smith

Clinical Research Faculty Publications

Objective: This study tested the hypothesis that treatment with the glucagon-like peptide-1/glucagon receptor agonist SAR425899 would lead to a smaller decrease in sleeping metabolic rate (SMR; kilocalories/day) than expected from the loss of lean and fat mass (metabolic adaptation). Methods: This Phase 1b, double-blind, randomized, placebo-controlled study was conducted at two centers in inpatient metabolic wards. Thirty-five healthy males and females with overweight and obesity (age = 36.5 ± 7.1 years) were randomized to a calorie-reduced diet (−1000 kcal/d) and escalating doses (0.06-0.2 mg/d) of SAR425899 (n = 17) or placebo (n = 18) for 19 days. SMR was measured …

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Early Time-Restricted Eating Affects Weight, Metabolic Health, Mood, And Sleep In Adherent Completers: A Secondary Analysis, Felicia L. Steger, Humaira Jamshed, David R. Bryan, Joshua S. Richman, Amy H. Warriner, Cody J. Hanick, Corby K. Martin, Sarah Jeanne Salvy, Courtney M. Peterson Feb 2023

Early Time-Restricted Eating Affects Weight, Metabolic Health, Mood, And Sleep In Adherent Completers: A Secondary Analysis, Felicia L. Steger, Humaira Jamshed, David R. Bryan, Joshua S. Richman, Amy H. Warriner, Cody J. Hanick, Corby K. Martin, Sarah Jeanne Salvy, Courtney M. Peterson

Clinical Research Faculty Publications

Objective: Data are mixed on whether intermittent fasting improves weight loss and cardiometabolic health. Here, the effects of time-restricted eating (TRE) in participants who consistently adhered ≥5 d/wk every week were analyzed. Methods: Ninety patients aged 25 to 75 years old with obesity were randomized to early TRE (eTRE; 8-hour eating window from 07:00 to 15:00) or a control schedule (≥12-hour window) for 14 weeks. A per-protocol analysis of weight loss, body composition, cardiometabolic health, and other end points was performed. Results: Participants who adhered to eTRE ≥5 d/wk every week had greater improvements in body weight (−3.7 ± 1.2 …

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Impact Of Early Time-Restricted Eating On Diet Quality, Meal Frequency, Appetite, And Eating Behaviors: A Randomized Trial, Felicia L. Steger, Humaira Jamshed, Corby K. Martin, Joshua S. Richman, David R. Bryan, Cody J. Hanick, Sarah Jeanne Salvy, Amy H. Warriner, Courtney M. Peterson Feb 2023

Impact Of Early Time-Restricted Eating On Diet Quality, Meal Frequency, Appetite, And Eating Behaviors: A Randomized Trial, Felicia L. Steger, Humaira Jamshed, Corby K. Martin, Joshua S. Richman, David R. Bryan, Cody J. Hanick, Sarah Jeanne Salvy, Amy H. Warriner, Courtney M. Peterson

Clinical Research Faculty Publications

Objective: Time-restricted eating (TRE) can reduce body weight, but it is unclear how it influences dietary patterns and behavior. Therefore, this study assessed the effects of TRE on diet quality, appetite, and several eating behaviors. Methods: Adults with obesity were randomized to early TRE plus energy restriction (eTRE + ER; 8-hour eating window from 7:00 a.m. to 3:00 p.m.) or a control eating schedule plus energy restriction (CON + ER; ≥12-hour window) for 14 weeks. Food intake was assessed via the Remote Food Photography Method, while eating patterns, appetite, and eating behaviors were assessed via questionnaires. Results: A total of …

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Framework Of The Alu Subfamily Evolution In The Platyrrhine Three-Family Clade Of Cebidae, Callithrichidae, And Aotidae, Jessica M. Storer, Jerilyn A. Walker, Jasmine N. Baker, Shifat Hossain, Christian Roos, Travis J. Wheeler, Mark A. Batzer Feb 2023

Framework Of The Alu Subfamily Evolution In The Platyrrhine Three-Family Clade Of Cebidae, Callithrichidae, And Aotidae, Jessica M. Storer, Jerilyn A. Walker, Jasmine N. Baker, Shifat Hossain, Christian Roos, Travis J. Wheeler, Mark A. Batzer

Faculty Publications

The history of Alu retroposons has been choreographed by the systematic accumulation of inherited diagnostic nucleotide substitutions to form discrete subfamilies, each having a distinct nucleotide consensus sequence. The oldest subfamily, AluJ, gave rise to AluS after the split between Strepsirrhini and what would become Catarrhini and Platyrrhini. The AluS lineage gave rise to AluY in catarrhines and to AluTa in platyrrhines. Platyrrhine Alu subfamilies Ta7, Ta10, and Ta15 were assigned names based on a standardized nomenclature. However, with the subsequent intensification of whole genome sequencing (WGS), large scale analyses to characterize Alu subfamilies using the program COSEG identified entire …

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The Role Of Nutrition In Mitigating The Effects Of Covid-19 From Infection Through Pasc, Jacob T. Mey, John P. Kirwan, Christopher L. Axelrod Feb 2023

The Role Of Nutrition In Mitigating The Effects Of Covid-19 From Infection Through Pasc, Jacob T. Mey, John P. Kirwan, Christopher L. Axelrod

Division of Basic Science Faculty Publications

The expansive and rapid spread of the SARS-CoV-2 virus has resulted in a global pandemic of COVID-19 infection and disease. Though initially perceived to be acute in nature, many patients report persistent and recurrent symptoms beyond the infectious period. Emerging as a new epidemic, “long-COVID”, or post-acute sequelae of coronavirus disease (PASC), has substantially altered the lives of millions of people globally. Symptoms of both COVID-19 and PASC are individual, but share commonality to established respiratory viruses, which include but are not limited to chest pain, shortness of breath, fatigue, along with adverse metabolic and pulmonary health effects. Nutrition plays …

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Identification Of Differential-Expressed Genes In Banana-Biostimulant Interaction Using Suppression Subtractive Hybridization, Tatiana Chavez-Navarrete, Luis Sanchez-Timm, Ricardo Pacheco-Coello, Niranjan Baisakh, Efrén Santos-Ordóñez Feb 2023

Identification Of Differential-Expressed Genes In Banana-Biostimulant Interaction Using Suppression Subtractive Hybridization, Tatiana Chavez-Navarrete, Luis Sanchez-Timm, Ricardo Pacheco-Coello, Niranjan Baisakh, Efrén Santos-Ordóñez

Faculty Publications

The use of environmental-friendly agricultural inputs to increase yield in crops could reduce environmental contamination and alleviate (a)biotic stress. In this study, we applied a foliar liquid biostimulant to banana plants, which was developed from the anaerobic fermentation of microorganisms with organic remains (manure and plant waste) and minerals to use as environmental-friendly product. To determine the effect of this biostimulant in the plants, a Suppression Subtractive Hybridization (SSH) library was developed from banana leaves in greenhouse conditions. Furthermore, salicylic and jasmonic pathways activation was evaluated by gene expression analysis. First, the application of the biostimulant to banana leaves increased …

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