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Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio Jan 2002

Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio

LSU Doctoral Dissertations

A series of experiments were conducted to produce transgenic goats by somatic cell nuclear transfer (NT). In all experiments, donor cells were electrically fused to enucleated metaphase II oocytes, then chemically activated. In a preliminary study to evaluate embryonic development following NT with proliferating or quiescent fibroblast or cumulus cells, significantly more embryos reconstructed with quiescent cumulus cells fused (77%) compared with proliferating cumulus cells (41%), proliferating fibroblasts (36%) or quiescent fibroblasts (37%). Improved development to the eight- to sixteen-cell stage was observed when fibroblast cells were serum starved (serum starved 39% vs. serum fed 15%). However, there was no …

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A Variable Response Time Lag Module For Car Following Models Using Fuzzy Set Theory, Yilmaz Hatipkarasulu Jan 2002

A Variable Response Time Lag Module For Car Following Models Using Fuzzy Set Theory, Yilmaz Hatipkarasulu

LSU Doctoral Dissertations

Since the 1950s, car following phenomena have been studied and analyzed, resulting in various models and algorithms. In general, the car following process has been defined as a stimulus-response relationship in which the driver of the following vehicle reacts to the actions of the lead vehicle after a time lag. One of the fundamental assumptions that underlie car following theory is that the driver response time lag is always a constant value for the driver at all times, regardless of level of detail of the model. Assumption of a constant time lag value introduces a number of broad assumptions however, …

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Synthesis And Study Of New Materials For The Visual Detection Of Biomolecules, Ming He Jan 2002

Synthesis And Study Of New Materials For The Visual Detection Of Biomolecules, Ming He

LSU Doctoral Dissertations

The visual determination of saccharides has been of interest for well over a century. Sugars are a relatively challenging class of compounds to analyze. They exhibit great structural similarity as well as transparency in the visible region. The sensing of specific saccharides could aid the monitoring of disease or industrial fermentation products. Herein I present new methods allowing for simple and rapid visual detection of sialic acid, fructose and a homologous series of prototypical neutral oligosaccharides, the linear maltodextrins, containing up to seven glucose residues. The new methods allow for selective color detection of fructose in a large excess of …

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Use Of Actigraphy To Objectively Measure Motor Restlessness In Restless Legs Syndrome, Tai Anderson Istre Jan 2002

Use Of Actigraphy To Objectively Measure Motor Restlessness In Restless Legs Syndrome, Tai Anderson Istre

LSU Doctoral Dissertations

Restless Legs Syndrome (RLS) is a disorder characterized by disagreeable leg sensations, usually prior to sleep onset, which cause an almost irresistible urge to move the legs. A characteristic feature of this disorder is that the movements are partially or completely relieved with leg motions. Attempts to find the underlying pathology have been unsuccessful. Thus, there are no objective physiological tests to diagnose this condition. Using the Suggested Immobilization Test (SIT), the current study attempted to validate a new and practical method for quantifying the motor symptoms of RLS, actigraphy. To this end, the SIT with actigraphy was evaluated for …

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Fast Scalable Visualization Techniques For Interactive Billion-Particle Walkthrough, Xinlian Liu Jan 2002

Fast Scalable Visualization Techniques For Interactive Billion-Particle Walkthrough, Xinlian Liu

LSU Doctoral Dissertations

This research develops a comprehensive framework for interactive walkthrough involving one billion particles in an immersive virtual environment to enable interrogative visualization of large atomistic simulation data. As a mixture of scientific and engineering approaches, the framework is based on four key techniques: adaptive data compression based on space-filling curves, octree-based visibility and occlusion culling, predictive caching based on machine learning, and scalable data reduction based on parallel and distributed processing. In terms of parallel rendering, this system combines functional parallelism, data parallelism, and temporal parallelism to improve interactivity. The visualization framework will be applicable not only to material simulation, …

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Artificial Peptides Containing Ca,A- Disubstituted Amino Acids: Synthesis, Conformational Studies, And Application As Β-Strand Mimics, Yanwen Fu Jan 2002

Artificial Peptides Containing Ca,A- Disubstituted Amino Acids: Synthesis, Conformational Studies, And Application As Β-Strand Mimics, Yanwen Fu

LSU Doctoral Dissertations

Short peptides containing Cα,α-dipropylglycine (Dpg) at alternating sequence positions were synthesized and examined for conformational behavior. Peptide assembly was performed using Fmoc-solid-phase chemistry where the coupling with PyAOP could be significantly enhanced at elevated temperature. Circular dichroism (CD) and NMR conformational studies revealed that incorporation of Dpg residues induced folded structures into peptides. It was observed that Dpg residues adopted helical conformation in a helix-promoting sequence. The resulting helical structure was comprised of consecutive β-turns whose structure was stabilized by salt bridge in aqueous solution. In this study, the preparation of sterically and polyfunctional Cα,α-disubstituted amino …

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Performing Hyphenates: A Study In Contemporary Irish-American Identity And Cultural Performance, Patrick Michael Bynane Jan 2002

Performing Hyphenates: A Study In Contemporary Irish-American Identity And Cultural Performance, Patrick Michael Bynane

LSU Doctoral Dissertations

This study examines the issues and contradictions of identity formation found in contemporary Irish-American cultural performances. Using a theoretical language grounded in post-structuralism and cultural studies, this examination hopes to demonstrate the primacy of performance and theatre in the formation of culture, Irish-American specifically, or otherwise. The performances featured in the study are: Riverdance, St. Patrick's Day parades, pub performances, and improv theatre.

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Ontogeny And Intervals Of Development In Five Reef-Associated Species Of Blenny From The Northern Gulf Of Mexico (Teleostei: Blenniidae), James G. Ditty Jan 2002

Ontogeny And Intervals Of Development In Five Reef-Associated Species Of Blenny From The Northern Gulf Of Mexico (Teleostei: Blenniidae), James G. Ditty

LSU Doctoral Dissertations

I examined patterns and timing of ontogeny and relative growth in five species of blenny (Teleostei: Blenniidae) from the northern Gulf of Mexico by assigning a suite of discrete character state scores to ontogenetic events (10 external traits; 218 total specimens). This is the first study to evaluate developmental patterns in reef-associated fishes relative to the timing of metamorphosis and settlement by applying scaling techniques and statistical methods to quantify, differentiate, and select criteria for defining intervals of development across taxa. Blennies settle at a common state of ontogeny and share a common pattern of body and fin/cirrus growth. Three …

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Characterization Of Chitinase Activities, And Cloning, Analysis, And Expression Of Genes Encoding Pathogenesis-Related Proteins In Strawberry, Anwar Ahmad Khan Jan 2002

Characterization Of Chitinase Activities, And Cloning, Analysis, And Expression Of Genes Encoding Pathogenesis-Related Proteins In Strawberry, Anwar Ahmad Khan

LSU Doctoral Dissertations

The goal of this dissertation research is to investigate the defense systems of strawberry plant by characterizing the genes and their products involved in plant disease-resistance. Pathogenesis-related proteins, including hydrolytic enzymes chitinases and â-1,3- glucanases, have been known to be induced in plants upon infection with various pathogens. Highest total chitinase activity was found in strawberry crown, whereas root, petiole, fruit, leaf, and runner showed successively lower activities. Chitinase isoform analysis showed that up to six acidic and two basic isoforms were present in various organs. The total chitinase activity was stable at 50oC. The pH optimum for chitinase activity …

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Techniques To Explore Time-Related Correlation In Large Datasets, Sumeet Dua Jan 2002

Techniques To Explore Time-Related Correlation In Large Datasets, Sumeet Dua

LSU Doctoral Dissertations

The next generation of database management and computing systems will be significantly complex with data distributed both in functionality and operation. The complexity arises, at least in part, due to data types involved and types of information request rendered by the database user. Time sequence databases are generated in many practical applications. Detecting similar sequences and subsequences within these databases is an important research area and has generated lot of interest recently. Previous studies in this area have concentrated on calculating similitude between (sub)sequences of equal sizes. The question of unequal sized (sub)sequence comparison to report similitude has been an …

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Study Of A Bacillus Circulans Chitin-Binding Domain By A Green Fluorescent Protein Binding Assay And Detection Of Lysozymes By Improved Zymograms, Markus Hardt Jan 2002

Study Of A Bacillus Circulans Chitin-Binding Domain By A Green Fluorescent Protein Binding Assay And Detection Of Lysozymes By Improved Zymograms, Markus Hardt

LSU Doctoral Dissertations

A fluorescent binding assay was developed to investigate the effects of site-directed mutagenesis on the binding affinity and binding specificity of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12. The chitin-binding domain (ChBD) was genetically fused to the N-terminus of the green fluorescent protein, GFP. The polyhistidine-tagged hybrid protein was expressed in Escherichia coli under the dose-dependent regulation of the araBAD promoter and purified using metal affinity-, chitin- or ion-exchange chromatography. Residues suggested to be involved in binding from previous three-dimensional studies were mutated and their contributions to binding and substrate specificity were evaluated by depletion assays. Purified …

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Infertility And Marital Adjustment: The Influence Of Perception Of Social Support, Privacy Preference And Level Of Depression, Margaret Haviland Maillet Jan 2002

Infertility And Marital Adjustment: The Influence Of Perception Of Social Support, Privacy Preference And Level Of Depression, Margaret Haviland Maillet

LSU Doctoral Dissertations

The purpose of this study was to investigate the influence of perception of social support, privacy preference and level of depression on marital adjustment for couples presenting for infertility treatment. Data was obtained from three infertility clinics in Louisiana from January 1, 2001 through October 1, 2001. Forty-seven couples presenting for infertility treatment completed four questionnaires measuring marital adjustment, perception of social support, privacy preference and level of depression. Demographic and descriptive information was gathered from an information sheet completed by the subjects. Multiple regression analysis was used to determine which, if any, of the independent variables predicted the dependent …

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Scientific And Management Perspectives In Wetland Groundwater Hydrology, Lorna Veronica Inniss Jan 2002

Scientific And Management Perspectives In Wetland Groundwater Hydrology, Lorna Veronica Inniss

LSU Doctoral Dissertations

Wetland groundwater hydrology was investigated at different spatial scales to assess the usefulness of the information to coastal managers. Specific objectives were to: (1) review studies related to coastal groundwater discharge, evaluating techniques and identifying controls; (2) understand regional trends in groundwater flow along the U.S. East and Gulf coasts; (3) evaluate the applicability of naturally occurring radioisotopes as indicators of groundwater in a shallow deltaic system; and (4) evaluate groundwater-surface water exchange within Barataria Basin, Louisiana. Results of the review confirmed discharge estimates vary over several orders of magnitude, due to differences in precipitation and tidal prisms. In addition, …

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Plasmid-Mediated Expression Of Foreign Genes In Ehrlichia Canis, Langston Dolphus Hull Jan 2002

Plasmid-Mediated Expression Of Foreign Genes In Ehrlichia Canis, Langston Dolphus Hull

LSU Doctoral Dissertations

Ehrlichia canis is the etiologic agent of “tropical canine pancytopenia”, or canine ehrlichiosis. The impetus for this research was to overcome the lack of any reliable means of elucidating the genetic profiles of these illusive and historically difficult to manipulate organisms. The use of a broad-host range plasmid greatly facilitated the determination of an electro-transformation protocol. The transforming plasmid possesses a chloramphenicol antibiotic resistance gene marker (chloramphenicol acetyltransferase [CAT] gene), and a visual reporter gene marker, the Green Fluorescent Protein (GFP) gene. With primer sets designed to specifically amplify these two plasmid encoded gene markers, thus verifying the presence of …

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Retention And Movement Of Reactive Chemicals In Soils, Hongxia Zhu Jan 2002

Retention And Movement Of Reactive Chemicals In Soils, Hongxia Zhu

LSU Doctoral Dissertations

Adsorption-desorption and transport behavior of pesticides are important processes that influence the amount retained by the soil and that which is susceptible to runoff or movement within the soil profile. Kinetic batch experiments were used to study the adsorption-desorption behavior of metolachlor (a non-ionic herbicide) and deltamethrin (a pyrethroid insectcide) in soils, and atrazine (a triazine) in sugarcane mulch residue. Miscible displacement experiments under steady flow conditions were carried out to examine the mobility of these pesticides in soils. Metolachlor adsorption and desorption by Sharkey clay soil(Very-fine, smectitic, thermic Chromic Epiaquerts) and Commerce silty loam soil (Fine-silty, mixed, superactive, nonacid, …

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Spatial Models Of Hawaiian Streams And Hawaiian Stream Fish Habital, James Elliott Parham Jan 2002

Spatial Models Of Hawaiian Streams And Hawaiian Stream Fish Habital, James Elliott Parham

LSU Doctoral Dissertations

A series of spatially based models of Hawaiian streams and stream fish habitats were developed to aid in the conservation of native fishes. The spatial models focused on the quantification of habitat for native fishes at three levels within a spatial hierarchy. First, at the reach level, the models classified the major geomorphological features of a stream and its watershed to determine the distribution of fish habitat within the different sections of the stream. The distribution of habitat was combined with habitat suitability criteria and the migratory abilities of the native fishes to quantify available habitat and predict expected community …

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Costs And Benefits Of Daphnia Antipredator Behavior And Consequences On Community Stability, Wiebke Johanna Boeing Jan 2002

Costs And Benefits Of Daphnia Antipredator Behavior And Consequences On Community Stability, Wiebke Johanna Boeing

LSU Doctoral Dissertations

Predator-prey interactions are an important factor in energy transfer through food webs. To estimate effects of altered energy flow caused by costs and benefits of antipredator defenses and to study their implications for community stability, I used the planktivorous fish - Chaoborus - Daphnia system. In the laboratory, I found that clonal variance of Daphnia antipredator defenses was high, and that behavioral, morphological, and life-history defenses were uncoupled. To estimate the costs and benefits of antipredator defenses in the presence of predators, I conducted lake-enclosure (1-m diameter x 7-m deep) experiments in the field, taking advantage of two features of …

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Dynamic Econometric Modeling Of The U.S. Wheat Grain Market, Carlos Walter Robledo Jan 2002

Dynamic Econometric Modeling Of The U.S. Wheat Grain Market, Carlos Walter Robledo

LSU Doctoral Dissertations

Structural-time series models have not gained much ground in commodity market modeling despite the overwhelming popularity of time series approaches in forecasting and dynamic analyses. This dissertation contributes by applying developments in seasonal cointegration and structural-time series analysis (e.g., Zellner and Palm (1974); Hsiao (1997); Lee (1992); Franses and Kunst (1999); Ghysels and Osborn, 2001) to the study of agricultural commodity markets. The focus is on three research themes. The first theme investigates the role of cointegration and seasonal cointegration for market data, an issue considered timely because most applications assume deterministic seasonal components. The second issue breaks new ground …

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Modeling Canopy Structure Effects On Loblolly Pine Growth, Mauricio Jerez Rico Jan 2002

Modeling Canopy Structure Effects On Loblolly Pine Growth, Mauricio Jerez Rico

LSU Doctoral Dissertations

This study examined several aspects of canopy structure and their influence on growth of loblolly pine (Pinus taeda L.) plantations. Foliage distribution, crown hydraulic architecture, and the effect of chronological changes in canopy structure on stand development were explored as possible components of a future process-based model intended for management purposes. A model based on the Johnson’s SB distribution was developed to predict leaf area distribution of loblolly pine trees. This is a preliminary step for building a submodel capable of simulating chronological changes in canopy structure and stand growth. The model accurately predicted the cumulative distribution of leaf area …

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Molecular Phylogeny Of Melanospora And Diaporthales, And Population Genetics Of Dogwood Anthracnose Fungus, Ning Zhang Jan 2002

Molecular Phylogeny Of Melanospora And Diaporthales, And Population Genetics Of Dogwood Anthracnose Fungus, Ning Zhang

LSU Doctoral Dissertations

Unresolved phylogenetic problems in pyrenomycetes and population structure of an asexual plant pathogen (Discula destructiva) were investigated. Analyses based on DNA sequences of nuclear encoded small and large subunit RNA genes (SSU and LSU nrDNA) and deduced amino acid sequences of a subunit of RNA polymerase II gene (RPB2) indicated that five genera previously considered in Ceratostomataceae of Sordariales were related to Hypocreales. Melanospora (including the type) and a genus of Ceratostomataceae formed a basal clade, but monophyly of the five genera and of the genus Melanospora was rejected. Discula destructiva and four other Discula species were derived from within …

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A Potential Nutraceutical From Leuconostoc Mesenteroides B-742 (Atcc 13146); Production And Properties, Chang-Ho Chung Jan 2002

A Potential Nutraceutical From Leuconostoc Mesenteroides B-742 (Atcc 13146); Production And Properties, Chang-Ho Chung

LSU Doctoral Dissertations

There is an emerging market for functional oligosaccharides for use in foods. Currently, technology for the production of oligosaccharides is limited to extraction from plant sources, acid or enzymatic hydrolysis of polysaccharides or synthesis by transglycosylation reactions. Oligosaccharides also can be produced using a Leuconostoc fermentation and restricting the polymer size by the addition of maltose. Maltose limits the dextransucrase reaction, yielding high concentrations of a-glucooligosaccharides. Branched oligomers produced by this process were found to be readily catabolized by bifidobacteria and lactobacillus but were not readily utilized by either Salmonella sp., or E. coli, pointing toward their use in intestinal …

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Northern Pintail Nest Site Selection, Nest Success, Renesting Ecology And Survival In The Intensively Farmed Prairies Of Southern Saskatchewan: An Evaluation Of The Ecological Trap Hypothesis, Kenneth Daniel Richkus Jan 2002

Northern Pintail Nest Site Selection, Nest Success, Renesting Ecology And Survival In The Intensively Farmed Prairies Of Southern Saskatchewan: An Evaluation Of The Ecological Trap Hypothesis, Kenneth Daniel Richkus

LSU Doctoral Dissertations

Unlike most prairie nesting ducks (Anas spp.), the North American population of northern pintails (A. acuta, hereafter pintails) has failed to respond as expected to improved wetland conditions on the U.S. and Canadian prairies during the mid to late 1990s. My primary objectives were to test the “ecological trap hypothesis” on a landscape level by examining pintail nest site selection and nest success in a highly agricultural environment in southern Saskatchewan. I also used radiotelemetry to estimate renesting and breeding season survival rates of female pintails; two parameters that are important in productivity and life cycle models. Most (51%) pintail …

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Reconceiving Curriculum: An Historical Approach, Stephen Shepard Triche Jan 2002

Reconceiving Curriculum: An Historical Approach, Stephen Shepard Triche

LSU Doctoral Dissertations

This dissertation reconceives curriculum through an historical approach that employs Ludwig Wittgenstein’s later philosophy. Curriculum is more than the knowledge taught in school. Curriculum, as I a theorist conceives it, is concerned with the broader intellectual and ideological ways a society thinks about education. Hence, the current school curriculum’s focus on specific learning outcomes offers a limited view of the knowledge fashioned by a society, thereby offering an intellectual and social history that is highly selective. Wittgenstein’s concept of “language-games” offers curricularists a way to re-include some of these stories. The concept of curriculum emerges at the end of the …

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The Sound Of Meaning: Theories Of Voice In Twentieth-Century Thought And Performance, Andrew Mccomb Kimbrough Jan 2002

The Sound Of Meaning: Theories Of Voice In Twentieth-Century Thought And Performance, Andrew Mccomb Kimbrough

LSU Doctoral Dissertations

This dissertation addresses the problem of the denigration of the voice in poststructural theory and contemporary performance criticism. The problem has antecedents in twentieth-century language philosophy. Saussure defines language as a compendium of arbitrary words recognized according to the degrees of phonetic difference between them. Since for Saussure the arbitrary words of language also designate arbitrary concepts, he concludes that the sounds of words cannot be thought constituent of their sense. After Saussure, structuralism dislodges the voice from its privileged position in the phonologic discourses of Western thought. Poststructuralism views meaning as a product of socially constructed language systems, and …

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Odorant Receptors: Regulation, Signaling, And Expression, Michele Lynn Rankin Jan 2002

Odorant Receptors: Regulation, Signaling, And Expression, Michele Lynn Rankin

LSU Doctoral Dissertations

Olfactory signal transduction is initiated by binding of odorous ligands to G protein-coupled odorant receptors, resulting in the activation of G protein and second messengers that culminates in the generation of action potentials to relay this signal to the brain for odor recognition. This signaling cascade comprises the primary olfactory response. Odorant receptors comprise the largest multigene family identified to date, enabling the olfactory system to respond to an enormous pool of potential odorant molecules. This work focuses on the study of odorant receptors beyond the initiation of the primary olfactory response. The first set of experiments focuses on odorant …

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Adoption Of Best Management Practices In The Louisiana Dairy Industry, Noro C. Rahelizatovo Jan 2002

Adoption Of Best Management Practices In The Louisiana Dairy Industry, Noro C. Rahelizatovo

LSU Doctoral Dissertations

The traditional view of the agricultural community as a good steward of the environment has been challenged by increasing concerns about the complex relationship between agricultural production activities and environmental quality. Agriculture provides a large range of products to satisfy human needs. It has also been singled out as major source of water pollution. Largely improved surface water quality has been assessed in the U.S. since the enactment of the Clean Water Act. However, efforts to reduce water pollution continue, targeting discharges from identifiable sources of water pollution and diffused discharges from nonpoint sources. Agricultural producers are encouraged to voluntarily …

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Aspects Of Cognition In Human Mate Selection, Michael J. Stasio Jan 2002

Aspects Of Cognition In Human Mate Selection, Michael J. Stasio

LSU Doctoral Dissertations

Evolutionary perspectives on human mating have provided testable hypotheses regarding what qualities people desire in their mates and why they want them. One study was conducted to replicate previous findings in mate preference using a more sophisticated paired comparison methodology to develop scales. Paired comparison scaling generally replicated gender differences in mate preferences consistent with evolutionary predictions. Further, decision-making reaction time (ms) suggested the presence of an underlying psychological continuum of selection criteria. A series of studies were then conducted applying the information processing approach (IPA) to investigate attention and knowledge organization in mate preference. The dot-probe paradigm was used …

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Delayed Phytotoxicity Syndrome In Louisiana Rice Caused By The Use Of Thiobencarb Herbicide, Chiliang Chen Jan 2002

Delayed Phytotoxicity Syndrome In Louisiana Rice Caused By The Use Of Thiobencarb Herbicide, Chiliang Chen

LSU Doctoral Dissertations

Thiobencarb (TB), widely used for the control of broadleaf weeds, grasses, and sedges in rice fields, is considered safe for rice plants when used at recommended rates. TB's reductive dechlorination product, dechlorinated thiobencarb (DTB), is highly toxic to rice. TB is naturally transformed into DTB in field soils in certain areas in Japan and the United States. The resultant syndrome is called delayed phytotoxicity syndrome (DPS). This research was conducted to characterize DPS in Louisiana, to compare the toxicity of TB and DTB to rice, to determine uptake and retention rates of TB and DTB by rice, to confirm that …

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Control Of Two Perennial Grasses In Southern Turfgrasses, Ronald Eugene Strahan Jan 2002

Control Of Two Perennial Grasses In Southern Turfgrasses, Ronald Eugene Strahan

LSU Doctoral Dissertations

Knotgrass (Paspalum distichum L.) and torpedograss (Panicum repens L.) are perennial grasses that are extremely difficult to remove in established turfgrass. Studies were conducted to determine the potential for controlling these troublesome perennial weeds. Pot studies were conducted for two years to evaluate several herbicides with multiple modes of action for knotgrass control. In graminicides studies, 8 weeks after initial application (WAI) single or split applications of fluazifop or quizalofop provided excellent control (88 to 95%). In contrast, single or split applications of fenoxaprop at 0.21 kg ha-1 and single applications of diclofop at 0.84 kg ha-1 were no better …

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The Post-Translational Methylation Of Arginine In The Glycine Arginine Rich Region Of Cho Nucleoin, Gregory James Pellar Jan 2002

The Post-Translational Methylation Of Arginine In The Glycine Arginine Rich Region Of Cho Nucleoin, Gregory James Pellar

LSU Doctoral Dissertations

Nucleolin is a nucleolar protein important for ribosome biogenesis. Nucleolin contains a conserved glycine arginine rich (GAR) domain near its carboxy terminus. GAR domains are defined by repeating tri-peptide, (arginine -glycine-glycine (RGG)) motifs. The arginine in the RGG motif is post-translationally modified by dimethylation on one of the two guanido nitrogens. Although arginine methylation was identified over 30 years ago, the function of this modification remains unknown. The GAR domain of nucleolin is important for nucleic acid binding and for nucleolar localization of nucleolin. This dissertation describes investigates possible in vivo interactions between nucleolin and other nucleolar proteins involved in …

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