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Biochemical And Biophysical Studies Of Wild-Type And Active-Site Mutants Of Human Adenosine Deaminase., Qing Zeng Jan 1996

Biochemical And Biophysical Studies Of Wild-Type And Active-Site Mutants Of Human Adenosine Deaminase., Qing Zeng

LSU Historical Dissertations and Theses

The focus of this work is structural and functional studies of wild-type and active-site mutants of human adenosine deaminase. Two active-site amino acids, Glu217 and His238, were previously substituted with alanine by site-directed mutagenesis. The Glu217Ala and His238Ala mutants showed little or no changes in Zn content and $\rm K\sb{m}$ value, yet dramatic loss of enzyme activity. Circular dichroism studies of the solution conformation of wild-type and mutant human adenosine deaminases indicate that 30 to 50% of $\alpha$-helical structure is lost in Glu217Ala and His238Ala, respectively, whereas $\beta$-sheet structure is fully retained. Inhibitor binding is measured by a fluorescent assay. …

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Toxicity Of Polycyclic Aromatic Hydrocarbons To Benthic Invertebrates., Guilherme R. Lotufo Jan 1996

Toxicity Of Polycyclic Aromatic Hydrocarbons To Benthic Invertebrates., Guilherme R. Lotufo

LSU Historical Dissertations and Theses

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in aquatic environments. Because of their hydrophobicity, PAHs accumulate in bed sediments and pose a risk to the benthos. Information on the toxicity of sediment-associated PAHs is, however, limited and more information is needed to improve sediment quality-criteria and ecological-risk assessments. My research focused on improving our current knowledge of the acute and, especially, the sublethal effects of sediment-associated PAHs to aquatic invertebrates. Representatives of two phylogenetically distinct benthic invertebrate groups, freshwater tubificid oligochaetes and estuarine meiobenthic harpacticoid copepods, were used. Organisms were exposed to a range of concentrations of sediment-amended contaminants in …

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Synthesis Of Uv/X-Ray Sensitive Polymers And Their Applications As Resists., Jack Dean Davies Jan 1996

Synthesis Of Uv/X-Ray Sensitive Polymers And Their Applications As Resists., Jack Dean Davies

LSU Historical Dissertations and Theses

Aliphatic polycarbonates were synthesized by condensation of the bischloroformate of 2,2,4,4-tetramethyl-1,3-cyclobutanediol with cyclic aliphatic diols. Polycarbonates that lacked flexible methylene units in the backbone processed T$\sb{\rm g}\ >$ 139$\sp\circ$C. Copolycarbonates acted as resists when combined with cobalt(III)-am(m)ine tetraphenylborate complexes which act as photointiators, with tetraphenylborate serving as counter anion. The photoinitiator liberates either ammonia or ethylene diamine base upon UV exposure and the polycarbonate backbone is subject to nucleophilic attack at the carbonyl linkage by the base. Image tone was dependent on the base used. Amine complexes, Co(NH$\sb3)\sb6\sp{3+}$ effected a positive tone due to ammonia induced main chain degradation at …

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Use Of Geographic Information System Technology To Assess Vulnerability To Shallow Groundwater Contamination., Brenda G. Bruner Jan 1996

Use Of Geographic Information System Technology To Assess Vulnerability To Shallow Groundwater Contamination., Brenda G. Bruner

LSU Historical Dissertations and Theses

Geographic Information System (GIS) technology was used to develop a local scale screening procedure for assessing shallow ground water's vulnerability to nitrate contamination. The procedure was based on the spatial distribution of ten key physical and chemical properties of soils. Both flow characteristics and attenuation capabilities of soils were incorporated into the assessment. Land surface areas were classified by the GIS to show the level of vulnerability present at a site as a result of existing natural conditions. The procedure produced a Vulnerability Index (VI), and resulted in VI ranging from 4.98 to 6.72 for the study area (with ten …

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Scale, Resolution And Resampling: Representation And Analysis Of Remotely Sensed Landscapes Across Scale In Geographic Information Systems., Stephanie Jean Weigel Jan 1996

Scale, Resolution And Resampling: Representation And Analysis Of Remotely Sensed Landscapes Across Scale In Geographic Information Systems., Stephanie Jean Weigel

LSU Historical Dissertations and Theses

Earth system scientists are increasingly using the technologies of Geographic Information Systems (GIS) and Remote Sensing (RS) in their analyses of earth system processes and patterns. These investigations take place over a wide range of scales, from the local to the global. Global change researchers focus on both the physical and human dimensions of changes in the earth's landscapes, which occur across a range of scales and may be scale dependent. The way in which landscapes are represented in GIS and RS, using specific spatial data models and data spatial resolutions, affects the subsequent analyses that can be performed. Optimally …

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A Methodology To Assess Safety Conditions In Louisiana Agriscience Laboratories., Julio Alcibiades Melendez Jan 1996

A Methodology To Assess Safety Conditions In Louisiana Agriscience Laboratories., Julio Alcibiades Melendez

LSU Historical Dissertations and Theses

The safety conditions of the agriscience laboratories of the high schools of the State of Louisiana have not been studied extensively. This study was undertaken to develop a methodology to assess these conditions. The Southeastern region of the state was selected as the research area. Forty four high schools in this area have agriscience laboratories. Forty one of these laboratories were assessed. A Hypothesized Survey Model was made up from the background information searched. This model was validated at another school region and used to design a Research Survey Model. This RSM was face and field validated. Safety ratings were …

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Development Of A Defined Minimal Medium For The Growth Of Edwardsiella Ictaluri, L. A. Collins, R. L. Thune Jan 1996

Development Of A Defined Minimal Medium For The Growth Of Edwardsiella Ictaluri, L. A. Collins, R. L. Thune

Faculty Publications

In this report, a complete defined medium and a minimally defined medium are described for Edwardsiella ictaluri. The complete defined medium consists of 46 individual components, including a basal salt solution, glucose, magnesium sulfate, iron sulfate, six trace metals, four nucleotides, 10 vitamins, and 19 amino acids. This medium supports growth in broth and on solid media. Optimal growth at 30°C was obtained at pH 7.0, and at an osmolality of 390 mosmol/kg of H2O, with a glucose concentration of 4 g/liter. The defined minimal medium reduces the 46 components of the complete medium to eight essential components, including the …

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Reconstructing Educational Experience: A Postmodern Perspective., Jeanne Edwards Robertson Jan 1996

Reconstructing Educational Experience: A Postmodern Perspective., Jeanne Edwards Robertson

LSU Historical Dissertations and Theses

Education in America today is dominated by the either/or thinking of modernism. This mode of thought is connected to both the concepts and methods of modern, mechanistic, Newtonian science. However, the attendant reductionistic methodology has been elevated beyond its realm of efficacy to the level of a world view, a metaphysical level. This is demonstrated by the omnipresence of the machine metaphor in all areas of human endeavor. The machine is the root metaphor of modernism. John Dewey speaks of a "new order of conceptions" necessary for significant change. These new order concepts are manifest in the new or postmodern …

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Cloning, Sequencing And Regulation Of The Rhodobacter Capsulatusheme Gene., Georgia Ineichen Jan 1996

Cloning, Sequencing And Regulation Of The Rhodobacter Capsulatusheme Gene., Georgia Ineichen

LSU Historical Dissertations and Theses

In Rhodobacter capsulatus, a purple nonsulfur bacterium, the tetrapyrrole pathway yields four end products: siroheme, vitamin B12, heme and bacteriochlorophyll. Growth of R. capsulatus under low oxygen tension increases carbon flow through the tetrapyrrole pathway up to 100 fold. The mechanism(s) for this regulation is currently unknown. One regulatory mechanism could be transcriptional control of one or more of the genes in the common portion of the tetrapyrrole pathway. The fifth gene in the pathway, hemE, has been cloned by complementation of an Escherichia coli hemE::miniTn10 mutant. Sequence data of hemE revealed the presence of an upstream palindrome that is …

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Importance Of A Siderophore In The Pathogenesis And Virulence Of Photobacterium Damsela Subsp. Piscicida In Hybrid Striped Bass (Morone Saxatili X Morone Chrysops)., John Phillip Hawke Jan 1996

Importance Of A Siderophore In The Pathogenesis And Virulence Of Photobacterium Damsela Subsp. Piscicida In Hybrid Striped Bass (Morone Saxatili X Morone Chrysops)., John Phillip Hawke

LSU Historical Dissertations and Theses

Photobacterium damsela subsp. piscicida, formerly Pasteurella piscicida, is an important new pathogen of hybrid striped bass cultured in brackish water on Louisiana mariculture farms. Louisiana isolates compared with strains of P. damsela subsp. piscicida from Greece, Japan, Israel and Chesapeake Bay, USA and were found to be identical in biochemical phenotype and enzymic activity. Small plasmids of 8 and 5 kb were unique to Louisiana strains. Sequential daily bacteriology and histopathology on hybrid striped bass experimentally infected by immersion revealed that following high doses (13,000 CFU/ml) the gills and spleen are colonized first as early as 24 hours PI, followed …

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The "Sacred Harp" In The Urban North: 1970-1995., Mark David Johnson Jan 1996

The "Sacred Harp" In The Urban North: 1970-1995., Mark David Johnson

LSU Historical Dissertations and Theses

In 1994 the Sacred Harp, a southern, shape-note tunebook, entered its 150th year of continuous use. First compiled in 1844 by B. F. White and E. J. King, and updated numerous times, The Sacred Harp books in the "Denson" tradition, including The Sacred Harp: 1991 Edition, are today the most popular and widely used twentieth-century revisions of any nineteenth-century tunebook. Sacred Harp singing has been a largely rural, Southern tradition and in the years between 1844 and the early 1970s several hundred separate singings were founded, many of which continue to be active. In the early 1990s the Sacred Harp …

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Amorphization And Fracture In Silicon Selenium(2) Nanowires: Molecular-Dynamics Simulations On Parallel Computer Architectures., Wei Li Jan 1996

Amorphization And Fracture In Silicon Selenium(2) Nanowires: Molecular-Dynamics Simulations On Parallel Computer Architectures., Wei Li

LSU Historical Dissertations and Theses

The primary goal of this dissertation is to investigate the structural and mechanical properties and dynamical fracture in SiSe$\sb2$ nanowires using the molecular-dynamics (MD) simulation technique. The present work is the first study of SiSe$\sb2$ nanowires. Large-scale simulations reported in this thesis are carried out with parallel multiresolution schemes for the long-range Coulomb and the three-body covalent potentials. The multiresolution scheme reduces the computational complexity from O(N$\sp2$) to O(N). Using an effective interatomic potential containing both 2- and 3-body interactions, MD simulations are performed for SiSe$\sb2$ nanowires composed of finite (1-64) number of chains. Under small uniaxial strain, the nanowires …

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Discontinuous Technological Change And Institutional Legitimacy: A Morphological Perspective., Robert Nicholas Mcgrath Jan 1996

Discontinuous Technological Change And Institutional Legitimacy: A Morphological Perspective., Robert Nicholas Mcgrath

LSU Historical Dissertations and Theses

Technological innovation is an important consideration to many strategic managers and thus is important to research in Strategic Management. Deepening the field's understanding of what technology is and how it works can be facilitated by participating in a growing dialogue between the Strategic Management and Management of Technology communities. This dissertation considers elements of both fields and examines the premise that at the industry level, incumbents act to manipulate the "share of mind" or "cognitive legitimacy" of the performance and cost/price characteristics of product-technology innovations that, in aggregate, portend to either enhance or destroy prevailing industry competences. A forecasting technique …

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Groundwater Flow With Heat And Solute Transport In Sedimentary Basins., Guichang Lin Jan 1996

Groundwater Flow With Heat And Solute Transport In Sedimentary Basins., Guichang Lin

LSU Historical Dissertations and Theses

Numerical experiments were undertaken to understand (1) heat and fluid transport in an uplifted foreland basin where fluid flow is driven by topographic gradient, and (2) effects of expulsion of geopressured fluids on the thermodynamic system in salt basins. Thermal evolution of an uplifted foreland basin is strongly affected by thermal buffering of basement rocks. Thermal buffering by basement rocks does not permit constant heat flow along the sediment-basement contact, in contrast to many previous numerical studies. Ignoring basement effects can result in serious errors in temperature prediction, such as a false transient thermal pulse in the discharge area. Carbonaceous …

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Modeling Physical And Chemical Nonequilibrium Transport Of Herbicide In Soils From Different Tillage Systems., Shikui Xue Jan 1996

Modeling Physical And Chemical Nonequilibrium Transport Of Herbicide In Soils From Different Tillage Systems., Shikui Xue

LSU Historical Dissertations and Theses

The physical and chemical nonequilibrium transport of alachlor were studied in a surface Gigger soil from different tillages through tracer studies, and batch and miscible displacement experiments. Batch experiments indicated initially fast reaction followed by slow adsorption. Adsorption and desorption results indicated time dependent hysteretic behavior and was best described by a multireaction model incorporating nonlinear equilibrium reaction, a reversible kinetic mechanism, and a consecutive irreversible mechanism. The model predicted alachlor hysteresis and adsorption-desorption kinetics satisfactorily based on parameters obtained from adsorption experiments. Tracer (Eosin Y and Blue FCF dyes) studies showed non-uniformly stained areas in undisturbed soil cores (6.4 …

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Children's Relative Influence In Purchase Decision-Making: A Multi-Theoretical Approach., Laura Ann Willis Williams Jan 1996

Children's Relative Influence In Purchase Decision-Making: A Multi-Theoretical Approach., Laura Ann Willis Williams

LSU Historical Dissertations and Theses

Sociological changes in the American family have positioned children to wield greater power in family purchase decisions. Although past descriptive research has confirmed that children are an important source of power in the household, this research has not explored the conceptual justification for the observed patterns of children's relative influence. To fill this gap in the family decision-making literature, this research develops a multi-theoretical conceptual model to explain children's relative influence in a purchase context. Social power theory, resource theory, social exchange theory, and social comparison theory were the conceptual frameworks for this research. Six studies were conducted, including a …

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Dynamic Storage Allocation Using Simon's Model Of Information Usage., Rachelle Folse Cope Jan 1996

Dynamic Storage Allocation Using Simon's Model Of Information Usage., Rachelle Folse Cope

LSU Historical Dissertations and Theses

In today's rapidly changing field of Management Information Systems (MIS) one problem faced by organizations is the consumption of storage capacity due to the growing base of software assets. Research has shown that very few firms effectively monitor program usage. Storage management issues arise due to the fact that many of the programs occupying valuable storage space are used infrequently. In this dissertation, we apply Simon's model of information usage in order to model the dynamic behavior of program usage. This methodology enables organizations to identify the changing usage frequencies of software assets. We propose a classification scheme which MIS …

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An Integrated Network Architecture For A High Speed Distributed Multimedia System., Nitin Subrao Naik Jan 1996

An Integrated Network Architecture For A High Speed Distributed Multimedia System., Nitin Subrao Naik

LSU Historical Dissertations and Theses

Computer communication demands for higher bandwidth and smaller delays are increasing rapidly as the march into the twenty-first century gains momentum. These demands are generated by visualization applications which model complex real time phenomena in visual form, electronic document imaging and manipulation, concurrent engineering, on-line databases and multimedia applications which integrate audio, video and data. The convergence of the computer and video worlds is leading to the emergence of a distributed multimedia environment. This research investigates an integrated approach in the design of a high speed computer-video local area network for a distributed multimedia environment. The initial step in providing …

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Set Redundancy, The Enhanced Compression Model, And Methods For Compressing Sets Of Similar Images., Kosmas Karadimitriou Jan 1996

Set Redundancy, The Enhanced Compression Model, And Methods For Compressing Sets Of Similar Images., Kosmas Karadimitriou

LSU Historical Dissertations and Theses

Image compression is the process of reducing the number of bits required to represent an image. This can be achieved by reducing (or ideally, eliminating) various types of redundancy that exist in the imaging data. All current image compression methods are based on the same theoretical model, which targets redundancy found in individual images. However, this model ignores an additional type of redundancy that exists in sets of similar images, the "set redundancy". The source of set redundancy is the common information existing in more than one image in a set of similar images. Set redundancy can be recognized by …

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An Examination Of Compositional Techniques In Selected Organ Solo Compositions Of Naji Hakim., Hope Alysia Davis Jan 1996

An Examination Of Compositional Techniques In Selected Organ Solo Compositions Of Naji Hakim., Hope Alysia Davis

LSU Historical Dissertations and Theses

Naji Hakim is one of the most talented Lebanese composers of the latter half of the twentieth century residing in France. His prolific output includes works for varied media including organ solos, organ duos, chamber music for varied instrument and voice combinations, guitar quartets, orchestral works, and oratorios. The goal of this study is to examine the compositional techniques used by the composer in five of his major published works for solo organ written between 1983 and 1993. The compositions chosen are Memor, Rubaiyat, Hommage a Igor Stravinsky, The Embrace of Fire and Symphonie en Trois Mouvements. Hakim's use of …

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Cytogenetic And Molecular Analysis Of The Channel Catfish (Ictalurus Punctatus) Genome., Quiyang Zhang Jan 1996

Cytogenetic And Molecular Analysis Of The Channel Catfish (Ictalurus Punctatus) Genome., Quiyang Zhang

LSU Historical Dissertations and Theses

This research presents studies on cytogenetics and molecular genetics of channel catfish (Ictalurus punctatus), the most important fish species cultured in the United States. The goals of this project were to analyze the genome of the channel catfish and to develop a direct method of mapping single-locus genes. A series of techniques were developed to facilitate these studies, including culture of fibroblast cells, preparation of chromosomes from catfish of different ages, staining for nucleolus organizer regions (NOR) and heterochromatin (C-banding), restriction enzyme banding, replication R-banding, simultaneous detection of sister-chromatid exchange (SCE) and C-banding, fluorescent in-situ hybridization (FISH) and indirect and …

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Women Who Lead High Schools: A Missing Element In Leadership Theory., Kristy Brock Hebert Jan 1996

Women Who Lead High Schools: A Missing Element In Leadership Theory., Kristy Brock Hebert

LSU Historical Dissertations and Theses

I addressed the problem that leadership theory is lacking in regard to how women lead high schools. My primary purpose was to contribute to the limited literature that exists regarding the leadership practices of women who are high school principals. I explored the notion of a female culture of school leadership and situated that culture among quintessential theory dictated by organizational research. I conducted this study in two phases. In Phase One I reported the demographics of 44 female high school principals in Louisiana along with their self-reported values on 32 leadership characteristics. Most important to their leadership were: communication, …

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Immobilization Of P. Digitatum And Bioconversion Of Limonene To Alpha-Terpineol., Qiang Tan Jan 1996

Immobilization Of P. Digitatum And Bioconversion Of Limonene To Alpha-Terpineol., Qiang Tan

LSU Historical Dissertations and Theses

The parameters of bioconversion of limonene to $\alpha$-terpineol by Penicillium digitatum were determined. This bioconversion was carried out stereo-specifically and selectively by this fungus. (R)-(+)-limonene was selectively converted to (R)-(+)-$\alpha$-terpineol by mycelia of this fungus during early growth phase. Immobilization of the fungal mycelia for use in continuous production of this product was possible. Aeration was critical to this bioconversion. The optimum aeration was about 1.6 mg/L for growing cells and immobilized cells. Substrate inhibition of the bioconversion was not observed when the substrate concentration was below 4% (v/v). End product inhibition was observed. The $\rm Ki\sb{app}$ was 12.08 mM. …

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Reduction And Management Of Vibrio Vulnificus In Gulf Coast Oyster (Crassostrea Virginica)., Yu-Pin Chen Jan 1996

Reduction And Management Of Vibrio Vulnificus In Gulf Coast Oyster (Crassostrea Virginica)., Yu-Pin Chen

LSU Historical Dissertations and Theses

The AmeriPure Process (AmeriPure Oyster Company, New Orleans, Louisiana), which is a mild heat treatment for a specific time period, proved effective in eliminating Vibrio vulnificus from the Gulf Coast oysters (Crassostrea virginica), thus reducing the risk of possible V. vulnificus infections. The treatment was effective in the reduction of V. vulnificus in both $1.5\times10\sp5$ MPN/g (high artificially contaminated level) and $2.4\times10\sp3$ MPN/g (low artificially contaminated level) to undetectable level (MPN $<$ 3/g). The mild heat treatment also reduced naturally occurring V. vulnificus $(8.9\pm3.7\times10\sp4$ MPN/g) present in shellstock oysters harvested in warmer months to undetectable levels. The D value for V. vulnificus present in shellstock oyster averaged 45.6 $\pm$ 3.3 seconds at the AmeriPure Process temperature. Mild heat treatment can also control aerobic plate count numbers during refrigerated storage (0-2$\sp\circ$C) and extend the shelf-life of raw oysters for at least 7 days over unprocessed samples. Mild heat treated oysters need to be refrigerated (${<}4\sp\circ$C) to ensure the safety and quality, as well as shelf-life. Oysters treated according to the AmeriPure Process when exposed to an average room temperature of 27$\sp\circ$C were found unacceptable after 24 hours due to the high number of aerobic plate count (10$\sp7$ CFU/g) and were considered not safe to eat. Although there was a slight lightening in color, sensory panels, composed of both traditional raw oyster consumers and infrequent consumers, found the treated product to be comparable in flavor, texture and smell to untreated oysters. According to the result of sensory evaluation, the good oyster flavor of treated samples increased and the off-flavor and off-odor of the treated oysters were significantly lower than the control samples after 7 days of storage on ice. Possible temperature variations, which could occur during the transportation and storage were studied in a temperature abuse study. The result showed that no Vibrio vulnificus was recovered and no significant microbiological quality change was observed after 10 hours of exposure to the room temperature of $22\pm2\sp\circ$C.

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Synthesis Of Novel Amino Acids With Incorporation Into Peptides And Synthesis Of Peptides With Novel Aggregation States., Scott Michael Cowell Jan 1996

Synthesis Of Novel Amino Acids With Incorporation Into Peptides And Synthesis Of Peptides With Novel Aggregation States., Scott Michael Cowell

LSU Historical Dissertations and Theses

Peptides exhibit properties based on the amino acids present in the peptide and structure the peptide assumes in its environment. For example, peptides have been shown to be antimicrobial when they are amphipathic and in an $\alpha$-helical shape. The mechanism for this antimicrobial activity is subject to dispute. One theory states that the peptides work by forming a "pore" through the cell membrane which causes a depolarization of the membrane and the cell lysis because of osmosis. Another theory states that the peptides merely compromise the integrity of the membrane by solubilizing the membrane. In order for the "pore" theory …

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"I Am Contemporary!": The Life And Times Of Penny Arcade., Matthew Sheridan Ames Jan 1996

"I Am Contemporary!": The Life And Times Of Penny Arcade., Matthew Sheridan Ames

LSU Historical Dissertations and Theses

"I Am Contemporary!": The Life and Times of Penny Arcade explores the career of performance artist Susana Ventura, a.k.a. Penny Arcade, and establishes her position in the American post-war avant-garde and counter-culture performance scene. The document is chronological, examining first Ventura's fifties childhood, moving toward her rebellious sixties, through her introspective seventies, and eventually into a performance career in the New York avant-garde scene of the eighties and nineties. Each main chapter explores three elements of these time periods, first establishing the social background, then moving to an overview of counter-cultural performance, and finally examining Ventura's work within that time …

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Polymerase Chain Reaction (Pcr) Assay For The Detection And Differentiation Of The Virulent Strains Of Mycoplasma Mycoides Subspecies Mycoides "Small Colony" Type From The Vaccine T1 Strain In Purified Dna And In Field Samples From Africa., Abdullahi Sheikh Hassan Kalif Jan 1996

Polymerase Chain Reaction (Pcr) Assay For The Detection And Differentiation Of The Virulent Strains Of Mycoplasma Mycoides Subspecies Mycoides "Small Colony" Type From The Vaccine T1 Strain In Purified Dna And In Field Samples From Africa., Abdullahi Sheikh Hassan Kalif

LSU Historical Dissertations and Theses

Two primer sets were manufactured from the cytadhesin P1 gene of M. Pneumoniae in an attempt to amplify homologous sequences in M. mycoides subsp. mycoides "SC" Type in a search for a P1-like gene in the latter organism. The first primer set MP-2 amplifies a 488 bp fragment in M. pneumoniae. The second primer set MP-3 amplifies a 303 bp fragment bracketing the 13-amino acid binding domain in the same organism. Both primer sets amplified the expected fragments in all the virulent strains of M. mycoides subsp. mycoides "SC" type. The primer sets did not however amplify the expected fragments …

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Chemical Effects On Microbial And Sensory Qualities Of Catfish In Planktonic And Dynamic Systems., Michael Adrain Land Jan 1996

Chemical Effects On Microbial And Sensory Qualities Of Catfish In Planktonic And Dynamic Systems., Michael Adrain Land

LSU Historical Dissertations and Theses

Listeria monocytogenes has the potential to be a serious pathogen for the compromised health segment of our population (immunocompromised, young, elderly and the pregnant). While the infectious dose is generally believed to be high 1.0 $\times$ 10$\sp6$, infectious dosages of 1.0 $\times$ 10$\sp3$ have been reported. Listeria monocytogenes poses a threat because of several factors: ubiquity in nature, isolation from fresh/salt waters, soil, ruminates and 17% of the human population. Listeria monocytogenes can colonize surfaces within 20 minutes, evade sanitizing, and is capable of growth from 37$\sp\circ$C to below refrigeration temperature. Fish and seafoods are high in water activity and …

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Growing Trees In A Greener World: Industrial Forestry In The 21st Century: 35th Lsu Forestry Symposium, 1996, School Of Forestry, Louisiana State University, Baton Rouge, Louisiana, Usa, Mason C. Carter Jan 1996

Growing Trees In A Greener World: Industrial Forestry In The 21st Century: 35th Lsu Forestry Symposium, 1996, School Of Forestry, Louisiana State University, Baton Rouge, Louisiana, Usa, Mason C. Carter

Proceedings [of the] Annual Forestry Symposium

No abstract provided.

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A Low-Co2-Inducible Gene Encoding An Alanine:Α-Ketoglutarate Aminotransferase In Chlamydomonas Reinhardtii, Zhi Yuan Chen, Mark D. Burow, Catherine B. Mason, James V. Moroney Jan 1996

A Low-Co2-Inducible Gene Encoding An Alanine:Α-Ketoglutarate Aminotransferase In Chlamydomonas Reinhardtii, Zhi Yuan Chen, Mark D. Burow, Catherine B. Mason, James V. Moroney

Faculty Publications

At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One full-length clone is 2552 bp, with an open reading frame encoding 521 amino acids. The deduced amino acid sequence shows about 50% identity with alanine:α-ketoglutarate aminotransferase (Ala AT, EC 2.6.1.2) from plants and animals, and the mRNA of this clone increased 4- to 5-fold 4 h after cells were switched from high-CO2 to low-CO2 growth conditions. The expression of the …

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