Digital Commons Network^™

Legacy Of Disunion: The Enduring Significance Of The American Civil War, Ward M. Mcafee

Civil War Book Review

Modern memories

Essay collection reflects the elusive legacy of the War

Evidence That Acetyl Phosphate Functions As A Global Signal During Biofilm Development, Alan J. Wolfe, Dong Eun Chang, Jason D. Walker, Jeanine E. Seitz-Partridge, Michael D. Vidaurri, Charles F. Lange, Birgit M. Prüß, Margaret C. Henk, John C. Larkin, Tyrrell Conway

Faculty Publications

We used DNA macroarray analysis to identify genes that respond to the status of the intracellular acetyl phosphate (acP) pool. Genes whose expression correlated negatively with the ability to synthesize acP (i.e. negatively regulated genes) function primarily in flagella biosynthesis, a result consistent with observations that we published previously (Prüß and Wolfe, 1994, Mol Microbiol 12: 973-984). In contrast, genes whose expression correlated positively with the ability to synthesize acP (i.e. positively regulated genes) include those for type 1 pilus assembly, colanic acid (capsule) biosynthesis and certain stress effectors. To our knowledge, this constitutes the first report that these genes …

A Perceptual Analysis Of Baton Rouge Media Entities: Professional Views Vs. Community Responses, Christopher Kaleel

Honors Capstones

No abstract provided.

Normal Proliferation And Differentiation Of Hoxc-8 Transgenic Chondrocytes In Vitro, Stephania A. Cormier, Maria Alice Mello, Claudia Kappen

Faculty Publications

Background: Hox genes encode transcription factors that are involved in pattern formation in the skeleton, and recent evidence suggests that they also play a role in the regulation of endochondral ossification. To analyze the role of Hoxc-8 in this process in more detail, we applied in vitro culture systems, using high density cultures of primary chondrocytes from neonatal mouse ribs. Results: Cultured cells were characterized on the basis of morphology (light microscopy) and production of cartilage-specific extracellular matrix (sulfated proteoglycans and type II Collagen). Hypertrophy was demonstrated by increase in cell size, alkaline phosphatase activity and type X Collagen immunohistochemistry. …

Genetic Organization And Iron-Responsive Regulation Of The Brucella Abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, A Cluster Of Genes Required For Wild-Type Virulence In Pregnant Cattle, Bryan H. Bellaire, Philip H. Elzer, Sue Hagius, Joel Walker, Cynthia L. Baldwin, R. Martin Roop

Faculty Publications

Brucella abortus reportedly produces the monocatechol siderophore 2,3-dihydroxybenzoic acid (2,3-DHBA) in response to iron limitation. Nucleotide sequence analysis of the cloned DHBA biosynthesis locus from virulent B. abortus 2308 and genetic complementation of defined Escherichia coli mutants were used to identify the B. abortus genes (designated dhbC, -B, and -A) responsible for synthesis of this siderophore. Reverse transcriptase PCR analysis of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are transcribed as components of an operon, together with dhbE, a functional homolog of the Escherichia coli entE gene. Homologs of the E. coli entD and Vibrio cholerae …

Louisiana Agriculture Spring, 2003, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Interstrain Inhibition In The Sweet Potato Pathogen Streptomyces Ipomoeae: Purification And Characterization Of A Highly Specific Bacteriocin And Cloning Of Its Structural Gene, Xiujun Zhang, Christopher A. Clark, Gregg S. Pettis

Faculty Publications

Strains of the sweet potato soil rot pathogen Streptomyces ipomoeae had previously been divided into three groups based on their ability to inhibit one another during pairwise cocultivation. While group I strains are not antagonistic to members of the other groups, group II and group III strains produce separate substances that are inhibitory to strains outside their respective cognate groups. Here, we purified the group III inhibitory substance from the culture supernatant of a representative strain and found that it consists of a single 10-kDa cationic protein which is bacteriolytic for S. ipomoeae group I and II strains but which …

Nonlinear Equations And Wavelets, A. Ludu, R. F. O'Connell, J. P. Draayer

Faculty Publications

Wavelet-inspired approaches for localized solutions of nonlinear partial differential equations (NDPE) were presented. A multi-scale similarity analysis was used which gives specific relations between the velocity, amplitude and width of localized solutions of nonlinear differential equations. This approach does not need the explicit form of the exact solutions.

Stat5a Promotes Adipogenesis In Nonprecursor Cells And Associates With The Glucocorticoid Receptor During Adipocyte Differentiation, Z. Elizabeth Floyd, Jacqueline M. Stephens

Faculty Publications

The differentiation of adipocytes is regulated by the activity of a variety of transcription factors, including peroxidase proliferator-activated receptor (PPAR)-γ and C/EBPα. Our current study demonstrates that ectopic expression of STAT5A, such as that of PPAR-γ and C/EBPα, promotes adipogenesis in two nonprecursor fibroblast cell lines. Using morphologic and biochemical criteria, we have demonstrated that STAT5A and the combination of STAT5A and STAT5B are sufficient to induce the expression of early and late adipogenic markers in BALB/c and NIH-3T3 cells. Yet, the ectopic expression of STAT5B alone does not induce the expression of adipocyte genes, but enhances the induction of …

Transcriptional Initiation Under Conditions Of Anoxia-Induced Quiescence In Mitochondria From Artemia Franciscana Embryos, Brian D. Eads, Steven C. Hand

Faculty Publications

In response to anoxia, embryos of the brine shrimp Artemia franciscana are able coordinately to downregulate metabolism to levels low enough to permit survival for several years at room temperature. In addition to dramatic decreases in free ATP levels and heat production, intracellular pH drops from 7.8 to 6.3 overnight. Use of isolated mitochondria to study transcriptional responses to anoxia offers several advantages: (1) the localized nature of transcript initiation, processing and degradation, all of which may be followed in organello; (2) the relatively simple cis- and trans-machinery involved and (3) the ability to provide relevant physiological treatments in vitro. …

Localization And Characterization Of Phenamil-Sensitive Na+ Influx In Isolated Rainbow Trout Gill Epithelial Cells, Scott D. Reid, G. S. Hawkings, F. Galvez, G. G. Goss

Faculty Publications

Percoll density-gradient separation, combined with peanut lectin agglutinin (PNA) binding and magnetic bead separation, was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each of the sub-populations was performed to determine which displayed acid-activated phenamil- and bafilomycin-sensitive Na+ uptake. Analysis of the mechanism(s) of 22Na+ influx was performed in control and acid-activated (addition of 10 mmoll-1 proprionic acid) cells using a variety of Na+ transport inhibitors (ouabain, phenamil, HOE-694 and bumetanide) and a V-type ATPase inhibitor (bafilomycin). We found that cells migrating to a 1.03-1.05 g ml-1 Percoll interface [pavement cells (PVCs)] possessed the lowest rates …

Plasma Membrane Topology Of Syncytial Domains Of Herpes Simplex Virus Type 1 Glycoprotein K (Gk): The Ul20 Protein Enables Cell Surface Localization Of Gk But Not Gk-Mediated Cell-To-Cell Fusion, Timothy P. Foster, Xavier Alvarez, Konstantin G. Kousoulas

Faculty Publications

Most spontaneously occurring mutations that cause extensive herpes simplex virus type 1 (HSV-1)-induced cell fusion are single amino acid changes within glycoprotein K (gK). Despite the strong genetic association of gK with virus-induced cell fusion, its direct involvement in cellular membrane fusion has been controversial, largely due to previously unsuccessful efforts to detect gK expression on virion and cellular surfaces. Recently, we showed that gK is expressed on HSV-1 virions and functioned in virus entry (T. P. Foster, G. V. Rybachuk, and K. G. Kousoulas, J. Virol. 75:12431-12438, 2001). To determine whether gK is expressed on cellular surfaces, as well …

Louisiana Agriculture Winter, 2003, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Louisiana Survey 2003, Lsu Reilly Center For Media & Public Affairs

Reilly Center Louisiana Surveys

The 2003 Louisiana Survey was the first in the series designed to establish benchmarks and assess progress and regressions in citizen assessments of state government services. In its inaugural report, the Louisiana Survey explored:

• perceptions on the most important problem facing the state
• evaluations of state performance, including comparisons between LA and other southeast state
• evaluations of personal, state, and national economic conditions
• taxes and spending
• Issues facing the state, such as open primaries and coastal preservation and restoration
• media use and attention to politics
• Louisiana public schools and education reform
• economic development and healthcare

Action Principle Formulation For The Motion Of Extended Bodies In General Relativity, Jeeva Anandan, Naresh Dadhich, Parampreet Singh

Faculty Publications

We present an action principle formulation for the study of the motion or an extended body in general relativity in the limit of a weak gravitational field. This gives the classical equations of motion for multipole moments of arbitrary order coupling to the gravitational field. In particular, a new force due to the octupole moment is obtained. The action also yields the gravitationally induced phase shifts in quantum interference experiments due to the coupling of all multipole moments. ©2003 The American Physical Society.

Philippe Jacques De Loutherbourg's A Midsummer's After-Noon With A Methodist Preacher Swedenborg, Wesley, And The Gospel According To Luke, Murray L. Brown

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Resource Evaluation: Hogarth And Durer Albercht Durer, Das Bruckgraphische Werk, Volume I: Kupferstiche, Eisenradierungen, Und Kaltnadelblatter Compiled By Rainer Schoch, Matthias Mende, And Anna Scherbaum Prestel (Munich) 2000, Peter Wagner

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Book Reviews

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Editor's Choice Underapplauded Books

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Book Reviews

1650-1850: Ideas, Aesthetics, and Inquiries in the Early Modern Era

No abstract provided.

Journals In The Discipline: A Report On A New Survey Of American Political Scientists, James C. Garand, Micheal W. Giles

Faculty Publications

No abstract provided.

A Regional Comparison Of Us Homeowner Perceptions About Treated Wood, Ben N. Donkor, Sanna Kallioranta, Richard P. Vlosky, Todd F. Shupe

Faculty Publications

This study examines United States homeowner perceptions about treated wood products. Regional differences were measured across a number of themes including overall perceptions about treated wood, treated wood relative to alternative building materials, and safety issues. Respondents in the South had the most positive perception of treated wood. The most significant health concerns were related to arsenic and chromium in preservative treatments with no differences across regions. Overall, there is not a strong aversion to using treated wood products per se although the perception of health risks exists.

Developing A User-Process Model For Designing Menu-Based Interfaces: An Exploratory Study, Boryung Ju, Myke Gluck

Faculty Publications

The purpose of this study was to organize menu items based on a user-process model and implement a new version of current software for enhancing usability of interfaces. Dervin & Nilan's time-line method is used to develop a user-process model for user interface design. We developed a user process model drawn from actual users' understanding of their goals and strategies to solve their information needs by using Dervin's Sense-Making Theory with sense-making interviews. Six experienced subjects were recruited for the sense-making interviews. Each subject went through a learning/exploration session, and was asked to recall and describe specific information needs throughout …

Effect Of Nacl On Growth, Morphology, And Camptothecin Accumulation In Camptotheca Acuminata Seedlings, Zhanhai Li, Zhijun Liu

Faculty Publications

NaCl salt is shown to increase alkaloid accumulation in some plant species, but creates an extensive problem in plant cultivation. The effect of NaCl on Camptotheca acuminata Decaisne growth, morphology, and camptothecin accumulation (concentration and yield) was studied in a hydroponic culture system. High NaCl levels (above 75 mM) in growth media significantly reduced plant height, weight, leaf number, and leaf length but increased leaf weight. In contrast, low NaCl levels (below 75 mM) only moderately reduced growth and affected morphology. Our results suggest a concentration of 75 mM is the threshold for NaCl to exert a detrimental effect on …

Insiders: Louisiana Journalists Sallie Rhett Roman, Helen Grey Gilkison, Iris Turner Kelso, Angie Pitts Juban

LSU Master's Theses

Sallie Rhett Roman, Helen Grey Gilkison and Iris Turner Kelso were three women journalists in Louisiana, active in consecutive time periods from 1891 to 1996. Their work brings up five particular questions. First, Why did these women start working and how did they negotiate public employment? Second, how did they balance the relationship between work and home since they did find employment outside of the home? Third, how did they fit into their contemporary image of women and journalists? Fourth, how did they use written language to portray a particular voice to the reader for a particular purpose? Fifth, did …

A Convergence Of Modes: Present Status Of Online News Sites A Content Analysis Of 100 Online Newspaper Web Sites, Renee Chantal Duplessis

LSU Master's Theses

This study examines the current status of online news sites in terms of their level of convergence, and how they are affected by different organizational factors such as organizational influence, cross media partnerships and circulation size. A content analysis of the top 100 circulated newspaper dailies in the United States was conducted to provide understanding as to the current status of these online newspaper sites. A number of categories were examined including newspaper circulation size, updatedness, cross media partnership/ ownership, and level of convergence. Results showed that the majority of the Internet newspaper sites examined shared a media partnership on …

Strength Properties Of Granular Materials, Brenda Novoa-Martinez

LSU Master's Theses

This thesis presents the results of the experimental work conducted on glass beads in order to investigate the effects of particle size, confining pressure, and surface roughness on the strength properties of the particulate media. Conventional triaxial compression tests were conducted to investigate those effects. Three different sizes of beads were tested: small (diameter = 0.75 – 1.00 mm), medium (diameter = 1.55 – 1.85 mm), and large (diameter = 3.30 – 3.60 mm). The glass beads were subjected to three different confining pressures: 25-, 100-, and 400-kPa. Smooth and etched beads were tested; the etched surface was achieved by …

Heat Transfer In Outdoor Aquaculture Ponds, Jonathan Lamoureux

LSU Master's Theses

An energy balance was developed for heated and unheated earthen aquaculture ponds to 1) determine the relative importance of energy transfer mechanism affecting pond temperature; 2) predict pond temperatures; 3) estimate the energy required to control pond temperatures, and 4) recommend efficient heating and cooling methods. PHATR (Pond Heating and Temperature Regulation), a computer program using 4th order Runge-Kutta numerical method was developed to solve the energy balance using weather, flow rate and pond temperature data. By comparing measured and modeled pond temperatures, the average difference (the average bias) was 0.5°C for unheated ponds and 2.4°C for heated ponds. The …

Measuring Fear Of Crime On Campus: A Study Of An Urban University, Cheryl Papa Bedenbaugh

LSU Master's Theses

Since 1990, five federal laws and many state laws have been created to increase security on university campuses (Security on Campus 2000). These laws, which include provisions that require university police and administrations to accurately and openly report the school's crime statistics (Hudge 2000), have fueled an increased focus about crime committed on university campuses. The philosophy behind the open reporting laws is twofold: parents and students have the information necessary to help them make the best decisions on which college to attend, and students are armed with information so they can take necessary precautions to enhance their level of …

Comparative Analysis Of State Environmental Enforcement In Region Six Of The United States, Rebecca Marcus

LSU Master's Theses

Through intense studies of agency audits, EPA policies and documents, state environmental compliance data and state agency issued documents, I have evaluated the enforcement programs of Region Six state environmental agencies. The four elements utilized to perform this evaluation were listed in EPA compliance assurance program documents. The analysis was conducted by comparing state program performance in each element. Element 1 is the “appropriate identification of violations.” Element 2 is the “timely issuance of enforcement actions.” Element 3 is the “escalation of enforcement actions when violations continue.” Element 4 is the “ability to assess and collect penalties.” These elements are …