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Analysis Of Bacterial Diversity And Biogeography At The Central Arizona-Phoenix Long Term Ecological Research (Cap Lter) Site, Brian Anthony Rash Jan 2004

Analysis Of Bacterial Diversity And Biogeography At The Central Arizona-Phoenix Long Term Ecological Research (Cap Lter) Site, Brian Anthony Rash

LSU Doctoral Dissertations

The limiting factor involved in past assessments of soil bacterial diversity when using culture-independent techniques has often been the lack of sampling and replication. As a result, analyses of community structural shifts across soil environments have lacked statistical power. In this study, 23 16S rRNA gene clone libraries consisting of over 11,000 clones were constructed from soils at the Central Arizona Phoenix Long Term Ecological Research (CAP LTER) site. Subsequent ARDRA fingerprinting and partial 16S rRNA gene sequencing allowed for a more robust investigation of various components that may explain any observed variations in bacterial species composition. The designated land …

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Performing Masculinities: U.S. Representations Of The Male Body In Performance Art Monologues, Darren C. Goins Jan 2004

Performing Masculinities: U.S. Representations Of The Male Body In Performance Art Monologues, Darren C. Goins

LSU Doctoral Dissertations

In this study, I describe and analyze the masculinities constructed in four performance art monologues staged in the US on Broadway. I examined Whoopi Goldberg’s 1984 performance Whoopi Goldberg Live, Lily Tomlin’s 1987 performance in Jane Wagner’s The Search for Signs of Intelligent Life in the Universe, Eric Bogosian’s 1990 performance Sex, Drugs, Rock & Roll, and John Leguizamo’s 1998 performance Freak: A Semi-Demi-Quasi-Pseudo Autobiography. My method of analysis is a critical interpretation incorporating the lenses of Robert Connell and Victor Seidler. It is grounded in a social-cultural perspective using Arthur W. Frank’s “sociology of the body.” By means of …

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"I Pray It Happens In My Lifetime": The Life History Of Clara Byrd Glasper, A Black Woman Educator Fighting For Educational Equality, Carol Marie Miller Jan 2004

"I Pray It Happens In My Lifetime": The Life History Of Clara Byrd Glasper, A Black Woman Educator Fighting For Educational Equality, Carol Marie Miller

LSU Doctoral Dissertations

The purpose of this study is to write the life history of a black woman educator is order to enhance our understandings of educational activism and social change in the Deep South. African American educators have been marginalized and under represented in their communities despite the roles they have played in ameliorating educational inequalities. The life history of seventy-year-old Clara Byrd Glasper’s activism as a black woman educator is one example. Life history, as a method of research, reveals an individual’s life experiences from their perspective and provides the appropriate methodology to explore the following research questions: In the context …

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Behavioral And Electrophysiological Assessment Of Children With A Specific Temporal Processing Disorder, Annette Hurley Jan 2004

Behavioral And Electrophysiological Assessment Of Children With A Specific Temporal Processing Disorder, Annette Hurley

LSU Doctoral Dissertations

Auditory processing disorders (APDs) have received considerable attention over the past few decades. Much of the attention has focused on the controversy surrounding the operational definition of APD, the heterogeneous nature of APD, and an appropriate test battery for APD assessment. Temporal processing deficits are one characteristic of APD and are the focus of the present investigation. This investigation reports behavioral and early electrophysiological measures in a group of children with specific temporal processing difficulties and an age-matched control group. In an effort to better describe the subjects, two language tests and the SCAN-C were administered. Significant differences were found …

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The Production Of A Dextran Binding Antibody By Phage Display Library And Its Applications To Sugar Processing, Duwoon Kim Jan 2004

The Production Of A Dextran Binding Antibody By Phage Display Library And Its Applications To Sugar Processing, Duwoon Kim

LSU Doctoral Dissertations

A phage collection (M1114), which is a mixture of two collections, M710-3R and Silica 426 each derived from a human synthetic phage antibody library (Fab 2lox), was used further enriched for dextran binding using enzyme-linked immunosorbent assays (ELISA) screening. The effects of dextran concentration on phage binding affinity were tested using indirect sandwich ELISA on phage collections, M1114-m74 and AE-M1114-m74-2R. Most of the phage bound to dextran (T2000) coated on a sandwich ELISA. The combination of ELISA screening and a sephadex columns enriched dextran binding 7 fold over enrichment by a single ELISA screen. Phage collection (M1114-m74) produced by combination …

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Botany In Children's Literature: A Content Analysis Of Plant-Centered Children's Picture Books That Have A Plot And Characters, Sheila Lewis Goins Jan 2004

Botany In Children's Literature: A Content Analysis Of Plant-Centered Children's Picture Books That Have A Plot And Characters, Sheila Lewis Goins

LSU Doctoral Dissertations

This content analysis study examined 36 plant-centered children’s science picture books that have a plot and characters published from 1950 to present. Botanical subject matter and learning opportunities offered by these books were analyzed, along with the range and frequency of the National Science Education Standards-consistent and age-appropriate plant science concepts and principles. The science graphics, artistic innovations, and story plot of these books were also examined. Rubrics and research-based recommendations were developed to offer parents, teachers, and librarians assistance in identifying, evaluating, and using such books to help children of ages 4-8 learn about plants and enjoy plant science. …

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A New Broad Spectrum Disinfectant Suitable For The Food Industry, Giovanna Aita Dequeiroz Jan 2004

A New Broad Spectrum Disinfectant Suitable For The Food Industry, Giovanna Aita Dequeiroz

LSU Doctoral Dissertations

A unique biocide composition (patent pending) that is formed from a hydrogen peroxide and sodium hypochlorite mixture was investigated. A biocidal "complex" is formed by adding the peroxide to the hypochlorite in an amount so that the weight ratio of the peroxide to the hypochlorite is no less than 1:10. The chemical structure of this biocidal "complex" is uncertain but we postulate that it is a semi-stable complex, whose stability is disrupted by heat, acid, U/V exposure and the presence of organic matter (i.e., microbes) The antimicrobial activity of the biocidal "complex" is most likely a combination effect between oxidation …

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The Organ Works Of Fela Sowande: A Nigerian Organist-Composer, Godwin Simeon Sadoh Jan 2004

The Organ Works Of Fela Sowande: A Nigerian Organist-Composer, Godwin Simeon Sadoh

LSU Doctoral Dissertations

Fela Sowande (1905-1987) has a huge compositional output including orchestral and vocal works. However, his organ music outnumbered the totality of his compositions. His organ pieces represent a truly intercultural music in which distinct tripartite cultural idioms are evident—Nigerian, African American and European. Works of this nature serve as creative source materials for aspiring composers, performers, scholars, music educators, and students in Africa and the world. This composer, folklorist, and music educator was born into a well-known music family in Nigeria. He was active in radio broadcasting, Yoruba folklore and mythology, indigenous music research, Nigerian art music, performance, orchestral conducting, …

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Material Length Scales In Gradient-Dependent Plasticity/Damage And Size Effects: Theory And Computation, Rashid Kamel Abu Al-Rub Jan 2004

Material Length Scales In Gradient-Dependent Plasticity/Damage And Size Effects: Theory And Computation, Rashid Kamel Abu Al-Rub

LSU Doctoral Dissertations

Structural materials display a strong size-dependence when deformed non-uniformly into the inelastic range: smaller is stronger. This effect has important implications for an increasing number of applications in structural failure, electronics, functional coatings, composites, micro-electro-mechanical systems (MEMS), nanostructured materials, micro/nanometer fabrication technologies, etc. The mechanical behavior of these applications cannot be characterized by classical (local) continuum theories because they incorporate no ‘material length scales’ and consequently predict no size effects. On the other hand, it is still not possible to perform quantum and atomistic simulations on realistic time and structures. It is therefore necessary to develop a scale-dependent continuum theory …

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What Irritates Consumers?: An Empirical Examination Of The Antecedents And Consequences Of Consumer Irritation, Sweta Chaturvedi Thota Jan 2004

What Irritates Consumers?: An Empirical Examination Of The Antecedents And Consequences Of Consumer Irritation, Sweta Chaturvedi Thota

LSU Doctoral Dissertations

This dissertation develops a model of consumer irritation in the context of consumer decision-making. Thus, the purpose is to describe and empirically test a model of the antecedents and consequences of consumer irritation. The model incorporates antecedents, moderators and consequences of irritation. It is suggested that irritation in consumers has a direct as well as an indirect influence, through retention of irritation in consumers, on the outcome variables such as attitude towards the advertised brand, and intentions to engage in negative word of mouth (NWOM) behavior. The central aim of this dissertation is to extend our understanding of the irritation …

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Flesh And Spirit Onstage: Chronotopes Of Performance In Medieval English Theatre, Gregory Lee Cavenaugh Jan 2004

Flesh And Spirit Onstage: Chronotopes Of Performance In Medieval English Theatre, Gregory Lee Cavenaugh

LSU Doctoral Dissertations

This study uses Mikhail Bakhtin's chronotope, which is the informing principle of one's experience of space and time, to explore different relations among space, time, actors, and audience in medieval theatre. Relations between the material and spiritual worlds as understood in the Middle Ages are considered in the context of relations between performers and audience members with two goals. First, I explore how the ontological status of the metaworld created through performance changed in the context of specific chronotopes. Second, I explore how diverse religious discourses affected medieval modes of representation. This study posits three chronotopes of performance informing medieval …

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A Theory Of Effective Computer-Based Instruction For Adults, Janis Sue Lowe Jan 2004

A Theory Of Effective Computer-Based Instruction For Adults, Janis Sue Lowe

LSU Doctoral Dissertations

Computer-based instruction (CBI) was considered the technological phenomenon to revolutionize education and training. Today, the Internet and computer technology are reported to have significantly altered the education landscape (Johnson & Aragon, 2002). The rapid advances in technology, the need for lifelong learning, and the growth of non-traditional students have encouraged the use of the computer as a method of instructional delivery. Evaluating the effectiveness of CBI as a whole technology is very difficult. The inability to measure effectiveness is attributable in part to the fact that CBI is not just one component, but a complex range of services and activities …

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The Control, Survival, And Growth Of Listeria Monocytogenes On Food Products, Richelle Lynn Beverly Jan 2004

The Control, Survival, And Growth Of Listeria Monocytogenes On Food Products, Richelle Lynn Beverly

LSU Doctoral Dissertations

Listeria monocytogenes, a ubiquitous foodborne pathogen, was recognized over 70 years ago. It is the source of the human disease listeriosis. The majority of Listeria monocytogenes that have been isolated from food product or human cases are of the serotypes ½ a, ½ b and 4b. Due to the recent outbreaks, recalls and deaths associated with Listeria monocytogenes in ready-to-eat meat products, the United States Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS) on October 2, 2003 issued a directive for the control of Listeria monocytogenes on ready-to-eat products. The ready-to-eat food industry must impose a post-lethality treatment …

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The Chick Embryo Amnion As An In Vitro Culture System For Ivf And Nt Embryos, Tonya Renea Davidson Jan 2004

The Chick Embryo Amnion As An In Vitro Culture System For Ivf And Nt Embryos, Tonya Renea Davidson

LSU Doctoral Dissertations

Calving rates are significantly reduced following in vitro production of embryos. Thus, if a technique could be developed that would increase calving rates by as little as one viable offspring, significant research advances could be made. Therefore, in a series of experiments, the efficiency and quality of culturing IVP bovine embryos in the amnion of a domestic chicken egg was tested. In Experiment I, by culturing IVP bovine embryos in the chick amnion (day 4 to 7 of incubation) it was discovered that there was no significant difference in blastocyst rates compared with controls. In Experiment II, it was shown …

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Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally Jan 2004

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally

LSU Doctoral Dissertations

The overall goals of this research were to develop a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed in situ hybridization as a method for the chromosomal gene detection in birds, development of Teflon-coated coverslip slides to facilitate laser microdissection of 0.5 ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on …

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Soils Of Regeneration: Exploring Conceptualizations Of The Natural World As A Context For An Ecologically-Sensitive Curriculum, Emily A. Demoor Jan 2004

Soils Of Regeneration: Exploring Conceptualizations Of The Natural World As A Context For An Ecologically-Sensitive Curriculum, Emily A. Demoor

LSU Doctoral Dissertations

David Orr (1994) asserts that the ecological crisis is a crisis of education. This study explores the relationship between the ecological crisis and education by examining the role that language plays in shaping perceptions of the natural world. Toward this end it analyzes narratives of science, literature and other disciplines that conceptualize the natural world as object and as subject. It evaluates how particular metaphors used in reference to the natural world enhance or impede ecological understanding and the cultivation of responsibility and stewardship and considers ways in which these conceptualizations might be used as a basis for new curriculum …

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Performance As Ministry: An Ethnographic Study Of Three Christian Repertory Theatre Troupes, Webster Ford Drake Jan 2004

Performance As Ministry: An Ethnographic Study Of Three Christian Repertory Theatre Troupes, Webster Ford Drake

LSU Doctoral Dissertations

This work seeks to define, explain, and place into historical and social context the phenomena of Christian Repertory Theater (CRT). It does so by examining three CRT troupes: Acts 2 from Nashville, TN, sponsored by Two Rivers Baptist Church; The Company from Fort Worth, TX, sponsored by Southwestern Baptist Theological Seminary; and Gen X from Clinton, MS, which operated independent of external support . Ethnographic fieldwork was the primary vehicle of information-gathering in this case study analysis. The author experienced each group as either a participant-observer, observer, and/or interviewer. CRT was ultimately defined as an activity wherein a constituted group …

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Social Protests, Asocial Media: Patterns Of Press Coverage Of Social Protests And The Influence Of The Internet Of Such Coverage, Sonora Jha Nambiar Jan 2004

Social Protests, Asocial Media: Patterns Of Press Coverage Of Social Protests And The Influence Of The Internet Of Such Coverage, Sonora Jha Nambiar

LSU Doctoral Dissertations

This dissertation examines media coverage of two social protests set more than three decades apart – “The March on the Pentagon” in October 1967, part of the anti-Vietnam war movement and “The Battle for Seattle” in November-December 1999, part of the movement for democratic globalization. Through two separate studies – a content analysis of print media coverage and qualitative in-depth interviews with journalists – this dissertation looks for patterns of sourcing and framing between the coverage of these two protests. It also examines any possible influence on these patterns caused by journalists’ access to diverse sources and research through the …

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Development Of A Predictive Model For Student-Athlete Retention And Graduation At Louisiana State University, Mary Allyn Boudreaux Jan 2004

Development Of A Predictive Model For Student-Athlete Retention And Graduation At Louisiana State University, Mary Allyn Boudreaux

LSU Doctoral Dissertations

The National Collegiate Athletic Association through its member institutions has implemented academic standards governing initial athletic eligibility and has led reform initiatives tying the ability to compete athletically to student-athlete retention and graduation. Louisiana State University (LSU), like many Division I institutions, admitted its scholarship athletes using these initial eligibility standards as a minimum qualification for admission. However, as NCAA requirements have become less stringent, the admissions requirements at LSU have increased. Concerns about the retention and graduation of student-athletes and an increasing gap between the academic credentials of the student body and student-athletes led administrators to question the wisdom …

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The Road To Redevelopment: New Urbanism, Nostalgia, And The Process Of Downtown Revitalization In Baton Rouge, Louisiana, Jennifer Speights-Binet Jan 2004

The Road To Redevelopment: New Urbanism, Nostalgia, And The Process Of Downtown Revitalization In Baton Rouge, Louisiana, Jennifer Speights-Binet

LSU Doctoral Dissertations

This qualitative study focuses on a planning process in Baton Rouge, Louisiana called Plan Baton Rouge, which began in the summer of 1998 and continues through the present. The overriding goal of the Plan Baton Rouge process is to revitalize downtown Baton Rouge and promote economic and cultural vitality while implementing the design practices of New Urbanism. New Urbanism is a design methodology that condemns suburban sprawl while promoting denser, early-twentieth-century-style townscapes and urban centers, focusing on mixed land use, pedestrianism, and aesthetic and architectural continuity. Through participant observation, this ethnographic account of New Urbanism in practice provides an in-depth …

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The Role Of Mentoring In The Development Of African American Nurse Leaders, Jacqueline Jones Hill Jan 2004

The Role Of Mentoring In The Development Of African American Nurse Leaders, Jacqueline Jones Hill

LSU Doctoral Dissertations

The primary purpose of this study was to explore the role of mentoring in the development of African American nurses who have achieved leadership positions in baccalaureate and graduate nursing programs. This study also explored the similar and dissimilar mentoring experiences in same-race versus cross-race mentoring relationships. The theoretical framework for this study is Levinson’s adult developmental theory. A sequential mixed design was utilized. Forty-seven African American nurse leaders participated in Phase I, of which 10 were interviewed in Phase II. The findings showed that mentoring plays a role in the personal and professional development of African American nurse leaders …

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Preservation Of Sperm Harvested From The Rat, Caprine, Equine, And Bovine Epididymis, Aida Nioma James Jan 2004

Preservation Of Sperm Harvested From The Rat, Caprine, Equine, And Bovine Epididymis, Aida Nioma James

LSU Doctoral Dissertations

The interest in preserving endangered species has increased the amount of attention lent to the recovery of functional sperm from the epididymides of deceased males (Foote, 2000). Postmortem specimens have a finite time period before decomposition affects functionality. Determination of this “window of opportunity” to harvest and preserve epididymal sperm would be beneficial for further research in sperm preservation and assisted reproductive technologies. The objective of these studies was to determine 1) the “window of opportunity” to collect viable rat, caprine, equine and bovine epididymal sperm, 2) if epididymal sperm collected could be cryopreserved, 3) to test two common cryoprotectants …

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Tissue Culture And Transformation Of Introducing Genes Useful For Pest Management In Rice, Shuli Zhang Jan 2004

Tissue Culture And Transformation Of Introducing Genes Useful For Pest Management In Rice, Shuli Zhang

LSU Doctoral Dissertations

Sheath blight (SB), caused by Rhizoctonia solani K¨¹hn, is a major rice disease internationally and in the southern rice area of the Unites States, including Louisiana. Breeders have incorporated partial resistance into commercial rice varieties to control the disease, but a higher level of resistance is needed. It has been demonstrated that the pathogenesis-related (PR) proteins ¦Â-1, 3-glucanase and chitinase are components of effective defense mechanisms for protecting plants against fungal pathogens. This research was conducted to co-transform the ¦Â-1, 3-glucanase, chitinase and bar genes into the rice variety Taipei 309 using the hpt gene for resistance to hygromycin B …

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A Genetic Study Of Resistance To Kernel Infection By Aspergillus Flavus In Maize (Zea Mays L.), Ruming Li Jan 2004

A Genetic Study Of Resistance To Kernel Infection By Aspergillus Flavus In Maize (Zea Mays L.), Ruming Li

LSU Doctoral Dissertations

Maize (Zea mays L.) kernel infection by Aspergillus flavus is a chronic problem in the southern USA. Genetic resistance to A. flavus is needed to solve this problem. To ascertain and understand the inheritance of resistance to field kernel infection by A. flavus, a five-parent diallel analysis and a half-sib family analysis of 35 maize crosses were conducted during 2003 and 2004 for percent kernel infection (PKI) rates. All parents contained the leafy gene (Lfy). From the combining ability analysis of the five maize parents and their 20 F1s, highly significant general combining ability (GCA), specific combining ability (SCA), and …

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A Comparison Of Administrator And Faculty Self-Report And Knowledge Of Distance Education, Related Intellectual Property Laws And Policy, And Tenets Of Academic Freedom, Mary Irene Pogue Jan 2004

A Comparison Of Administrator And Faculty Self-Report And Knowledge Of Distance Education, Related Intellectual Property Laws And Policy, And Tenets Of Academic Freedom, Mary Irene Pogue

LSU Doctoral Dissertations

Distance education is an emerging force in higher education that is creating new opportunities and added challenges. The purpose of this study was to identify and compare what university administrators and faculty know about issues that surround a debate about ownership of intellectual products created for distance education including technologies used in distance education and the law, university policies, and tenets of academic freedom that are supposed to stimulate intellectual creativity. An Internet-based survey was used to gather data from university faculty and administrators at four southeastern research universities in the United States. Results indicated that respondents were almost universally …

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Effects Of Gp130 Cytokines On Adipocytes, Sanjin Zvonic Jan 2004

Effects Of Gp130 Cytokines On Adipocytes, Sanjin Zvonic

LSU Doctoral Dissertations

Members of the gp130 cytokine family are known for their pleiotropic roles in various cell types. Our work has focused on their actions in adipocytes. CNTF administration has been shown to ameliorate most complications associated with obesity and type 2 diabetes through an unknown mechanism. In this study CNTF is shown to activate JAK/STAT and MAPK signaling, both in vitro and in vivo. In 3T3-L1 adipocytes, chronic CNTF also regulates the expression of SREBP-1, FAS, IRS-1 and GLUT4. Acute CNTF administration enhances the activation of IRS-1 and Akt by insulin. Our data also demonstrate that the expression of CNTF-specific receptor, …

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Quantification Of Marine Sediment Properties From Planar And Volumetric Pore Geometries, Allen Hagerman Reed Jan 2004

Quantification Of Marine Sediment Properties From Planar And Volumetric Pore Geometries, Allen Hagerman Reed

LSU Doctoral Dissertations

Pore geometry and topology are important determinants of sediment physical properties, such as porosity and permeability. They also influence processes that occur in the sediment, such as acoustic propagation, attenuation, and dispersion, single- and multi-phase fluid flow, and hydrodynamic dispersion. This study uses images to evaluate pore geometry and topology of ooid (subspherical particles) and siliclastic (angular quartz) sand that was collected from the marine environment south of Bimni Bahamas and Ft. Walton Beach, FL, respectively. Image analysis techniques and predictive tools enable insight into the relationships among sediment pore geometry, topology, and physical properties for these differently shaped sands. …

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From Oklahomans To "Okies": Identity Formation In Rural California, Toni Ann Alexander Jan 2004

From Oklahomans To "Okies": Identity Formation In Rural California, Toni Ann Alexander

LSU Doctoral Dissertations

Throughout the twentieth century difficult economic circumstances have resulted in reduced employment opportunities. In-migrants have long borne the brunt of these limitations, facing open hostilities from residents who felt that these "outsiders" were undeserving of employment and social services. Within the context of the 1930s Depression in the Central Valley of California, such negative public sentiment was often directed at "Okies," the 315,000 former residents of the "Western South" who crossed the California state line in search of employment in the agricultural fields of the Golden State. In this dissertation, I examine the changing conceptualizations of Okie identity throughout the …

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Agouti Expression In Human Adipose Tissue: Functional Consequences And Increased Expression In Type 2 Diabetes, Steven R. Smith, Barbara Gawronska-Kozak, Lenka Janderová, Taylor Nguyen, Angela Murrel, Jacqueline M. Stephens, Randall L. Mynatt Dec 2003

Agouti Expression In Human Adipose Tissue: Functional Consequences And Increased Expression In Type 2 Diabetes, Steven R. Smith, Barbara Gawronska-Kozak, Lenka Janderová, Taylor Nguyen, Angela Murrel, Jacqueline M. Stephens, Randall L. Mynatt

Faculty Publications

It is well recognized that the agouti/melanocortin system is an important regulator of body weight homeostasis. Given that agouti is expressed in human adipose tissue and that the ectopic expression of agouti in adipose tissue results in moderately obese mice, the link between agouti expression in human adipose tissue and obesity/type 2 diabetes was investigated. Although there was no apparent relationship between agouti mRNA levels and BMI, agouti mRNA levels were significantly elevated in subjects with type 2 diabetes. The regulation of agouti in cultured human adipocytes revealed that insulin did not regulate agouti mRNA, whereas dexamethasone treatment potently increased …

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Rubisco Activase Is Required For Optimal Photosynthesis In The Green Alga Chlamydomonas Reinhardtii In A Low-Co2 Atmosphere, Steve V. Pollock, Sergio L. Colombo, Davey L. Prout, Ashley C. Godfrey, James V. Moroney Dec 2003

Rubisco Activase Is Required For Optimal Photosynthesis In The Green Alga Chlamydomonas Reinhardtii In A Low-Co2 Atmosphere, Steve V. Pollock, Sergio L. Colombo, Davey L. Prout, Ashley C. Godfrey, James V. Moroney

Faculty Publications

This report describes a Chlamydomonas reinhardtii mutant that lacks Rubisco activase (Rca). Using the BleR (bleomycin resistance) gene as a positive selectable marker for nuclear transformation, an insertional mutagenesis screen was performed to select for cells that required a high-CO2 atmosphere for optimal growth. The DNA flanking the Ble R insert of one of the high-CO2-requiring strains was cloned using thermal asymmetric interlaced-polymerase chain reaction and inverse polymerase chain reaction and sequenced. The flanking sequence matched the C. reinhardtii Rca cDNA sequence previously deposited in the National Center for Biotechnology Information database. The loss of a functional Rca in the …

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