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Advances In Satellite Radiometry For The Surveillance Of Surface Temperatures, Ocean Eddies And Upwelling Processes In The Gulf Of Mexico Using Goes-8 Measurements During Summer, Nan Walker, Soe Myint, Adele Babin, Alaric Haag Aug 2003

Advances In Satellite Radiometry For The Surveillance Of Surface Temperatures, Ocean Eddies And Upwelling Processes In The Gulf Of Mexico Using Goes-8 Measurements During Summer, Nan Walker, Soe Myint, Adele Babin, Alaric Haag

Faculty Publications

GOES-8 satellite infrared (IR) measurements have been under-utilized in oceanographic research, although they provide several advantages over measurements from other sensors such as the NOAA AVHRR. Frequent coverage (48 times/day) over large regions, low noise in the mid- IR channel, and sole use of nighttime data have enabled the development of improved techniques for producing "de-clouded" images and calculating accurate SSTs (with RMS errors < 0.5°C), over cloudy and humid regions such as the Gulf of Mexico. Animations of SST composite images reveal movements of the Loop Current, its frontal eddies and coastal upwelling even during summer months. GOES-12 and future GOES imagers for the next decade are missing the 12 μm channel; however, this study demonstrates that the mid-IR channel is even more useful than previously thought.

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Development And Evolution Of The Amniote Integument: Current Landscape And Future Horizon, Cheng Ming Chuong, Dominique G. Homberger Aug 2003

Development And Evolution Of The Amniote Integument: Current Landscape And Future Horizon, Cheng Ming Chuong, Dominique G. Homberger

Faculty Publications

This special issue on the development and evolution of the amniote integument begins with a discussion of the adaptations to terrestrial conditions, the acquisition of water-impermeability by the reptilian integument, and the initial formation of filamentous integumentary appendages that pave the way towards avian flight. Recent feather fossils are reviewed and a definition of feathers is developed. Hierarchical models are proposed for the formation of complex structures, such as feathers. Molecular signals that alter the phenotype of integumentary appendages at different levels of the hierarchy are presented. Tissue interactions and the roles of keratins in evolution are discussed and linked …

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Recently Integrated Alu Elements And Human Genomic Diversity, Abdel Halim Salem, Gail E. Kilroy, W. Scott Watkins, Lynn B. Jorde, Mark A. Batzer Aug 2003

Recently Integrated Alu Elements And Human Genomic Diversity, Abdel Halim Salem, Gail E. Kilroy, W. Scott Watkins, Lynn B. Jorde, Mark A. Batzer

Faculty Publications

A comprehensive analysis of two Alu Y lineage subfamilies was undertaken to assess Alu-associated genomic diversity and identify new Alu insertion polymorphisms for the study of human population genetics. Recently integrated Alu elements (283) from the Yg6 and Yi6 subfamilies were analyzed by polymerase chain reaction (PCR), and 25 of the loci analyzed were polymorphic for insertion presence/absence within the genomes of a diverse array of human populations. These newly identified Alu insertion polymorphisms will be useful tools for the study of human genomic diversity. Our screening of the Alu insertion loci also resulted in the recovery of several "young" …

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Little Phillip No. 1 Bermudagrass (Research Information Sheet #106), W. D. Pitman Aug 2003

Little Phillip No. 1 Bermudagrass (Research Information Sheet #106), W. D. Pitman

LSU AgCenter Research Information Sheets

A unique, naturally occurring variety of Bermudagrass was identified in an Alicia Bermudagrass hay field in Sabine Parish in 1991. This publication includes information on the comparisons of this variety with the three Bermudagrass varieties most widely planted in the area in recent years, conducted in a field plot experiment at the Rosepine Research Station.

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Methacholine-Induced Airway Hyperresponsiveness Is Dependent On GαQ Signaling, Michael T. Borchers, T. Biechele, J. P. Justice, T. Ansay, S. Cormier, V. Mancino, T. M. Wilkie, M. I. Simon, N. A. Lee, J. J. Lee Jul 2003

Methacholine-Induced Airway Hyperresponsiveness Is Dependent On GαQ Signaling, Michael T. Borchers, T. Biechele, J. P. Justice, T. Ansay, S. Cormier, V. Mancino, T. M. Wilkie, M. I. Simon, N. A. Lee, J. J. Lee

Faculty Publications

Airway function in health and disease as well as in response to bronchospastic stimuli (i.e., irritants, allergens, and inflammatory mediators) is controlled, in part, by cholinergic muscarinic receptor regulation of smooth muscle. In particular, the dependence of airway smooth muscle contraction/relaxation on heterotrimeric G protein-coupled receptor signaling suggests that these events underlie the responses regulating airway function. Gαq-containing G proteins are proposed to be a prominent signaling pathway, and the availability of knockout mice deficient of this subunit has allowed for an investigation of its potential role in airway function. Airway responses in Gαq-deficient mice (activities assessed by both tracheal …

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Louisiana Agriculture Summer, 2003, Lsu Agcenter Jul 2003

Louisiana Agriculture Summer, 2003, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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Common And Distinguishing Regulatory And Expression Characteristics Of The Highly Related Korb Proteins Of Streptomycete Plasmids Pij101 And Psb24.2, Matthew J. Ducote, Gregg S. Pettis Jul 2003

Common And Distinguishing Regulatory And Expression Characteristics Of The Highly Related Korb Proteins Of Streptomycete Plasmids Pij101 And Psb24.2, Matthew J. Ducote, Gregg S. Pettis

Faculty Publications

The conjugative plasmid pIJ101 of the spore-forming bacterium Streptomyces lividans contains a regulatory gene, korB, whose product is required to repress potentially lethal expression of the pIJ101 kilB gene. The KorB protein also auto-regulates korB gene expression and may be involved in control of pIJ101 copy number. KorB (pIJ101) is expressed as a 10-kDa protein in S. lividans that is immediately processed to a mature 6-kDa repressor molecule. The conjugative Streptomyces cyanogenus plasmid pSB24.1 is deleted upon entry into S. lividans to form pSB24.2, a nonconjugative derivative that contains a korB gene nearly identical to that of pIJ101. Previous evidence …

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Chromatin Of Endoreduplicated Pavement Cells Has Greater Range Of Movement Than That Of Diploid Guard Cells In Arabidopsis Thaliana, Naohiro Kato, Eric Lam Jun 2003

Chromatin Of Endoreduplicated Pavement Cells Has Greater Range Of Movement Than That Of Diploid Guard Cells In Arabidopsis Thaliana, Naohiro Kato, Eric Lam

Faculty Publications

In the current model of chromatin organization in the interphase cell nucleus, chromosomes are organized into territories. Although constrained diffusion of chromatin in interphase cells has been confirmed in all cell types examined, little is known about chromatin dynamics in plant interphase cells. In this work, we measured for the first time interphase chromatin dynamics in plants using the green-fluorescent-protein-mediated chromatin-tagging system. Moreover, we compared the dynamics of diploid guard cells and endoreduplicated pavement cells. The movement of tagged loci in live seedlings shows constrained behavior in both types of nuclei. However, we found that the apparent confinement area for …

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Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady May 2003

Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady

Honors Capstones

No abstract provided.

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Production Of The Siderophore 2,3-Dihydroxybenzoic Acid Is Required For Wild-Type Growth Of Brucella Abortus In The Presence Of Erythritol Under Low-Iron Conditions In Vitro, Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, R. Martin Roop May 2003

Production Of The Siderophore 2,3-Dihydroxybenzoic Acid Is Required For Wild-Type Growth Of Brucella Abortus In The Presence Of Erythritol Under Low-Iron Conditions In Vitro, Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, R. Martin Roop

Faculty Publications

Production of the siderophore 2,3-dihyroxybenzoic acid (2,3-DHBA) is required for the wild-type virulence of Brucella abortus in cattle. A possible explanation for this requirement was uncovered when it was determined that a B. abortus dhbC mutant (BHB1) defective in 2,3-DHBA production displays marked growth restriction in comparison to its parent strain, B. abortus 2308, when cultured in the presence of erythritol under low-iron conditions. This phenotype is not displayed when these strains are cultured under low-iron conditions in the presence of other readily utilizable carbon and energy sources. The addition of either exogenous 2,3-DHBA or FeCl3 relieves this growth defect, …

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Linkage For Bmi At 3q27 Region Confirmed In An African-American Population, Amy Luke, Xiaodong Wu, Xiaofeng Zhu, Donghui Kan, Yan Su, Richard Cooper May 2003

Linkage For Bmi At 3q27 Region Confirmed In An African-American Population, Amy Luke, Xiaodong Wu, Xiaofeng Zhu, Donghui Kan, Yan Su, Richard Cooper

Faculty Publications

Significant and suggestive linkage for BMI on 3q27 has been reported by several groups, including our own study in African Americans. To further establish the linkage evidence on 3q27, we recruited an independent African-American sample comprising 545 individuals in 128 families. We genotyped 15 short tandem-repeat markers evenly spaced in the 112 cM region around the peak on 3q27 identified in our earlier study. Multipoint linkage analysis by GENEHUNTER2 gave the maximum logarithm of odds (LOD) score 2.4 at map position 188 cM in this sample. When we combined the two samples, linkage evidence was increased to a maximum LOD …

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Normal Proliferation And Differentiation Of Hoxc-8 Transgenic Chondrocytes In Vitro, Stephania A. Cormier, Maria Alice Mello, Claudia Kappen Apr 2003

Normal Proliferation And Differentiation Of Hoxc-8 Transgenic Chondrocytes In Vitro, Stephania A. Cormier, Maria Alice Mello, Claudia Kappen

Faculty Publications

Background: Hox genes encode transcription factors that are involved in pattern formation in the skeleton, and recent evidence suggests that they also play a role in the regulation of endochondral ossification. To analyze the role of Hoxc-8 in this process in more detail, we applied in vitro culture systems, using high density cultures of primary chondrocytes from neonatal mouse ribs. Results: Cultured cells were characterized on the basis of morphology (light microscopy) and production of cartilage-specific extracellular matrix (sulfated proteoglycans and type II Collagen). Hypertrophy was demonstrated by increase in cell size, alkaline phosphatase activity and type X Collagen immunohistochemistry. …

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Genetic Organization And Iron-Responsive Regulation Of The Brucella Abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, A Cluster Of Genes Required For Wild-Type Virulence In Pregnant Cattle, Bryan H. Bellaire, Philip H. Elzer, Sue Hagius, Joel Walker, Cynthia L. Baldwin, R. Martin Roop Apr 2003

Genetic Organization And Iron-Responsive Regulation Of The Brucella Abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, A Cluster Of Genes Required For Wild-Type Virulence In Pregnant Cattle, Bryan H. Bellaire, Philip H. Elzer, Sue Hagius, Joel Walker, Cynthia L. Baldwin, R. Martin Roop

Faculty Publications

Brucella abortus reportedly produces the monocatechol siderophore 2,3-dihydroxybenzoic acid (2,3-DHBA) in response to iron limitation. Nucleotide sequence analysis of the cloned DHBA biosynthesis locus from virulent B. abortus 2308 and genetic complementation of defined Escherichia coli mutants were used to identify the B. abortus genes (designated dhbC, -B, and -A) responsible for synthesis of this siderophore. Reverse transcriptase PCR analysis of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are transcribed as components of an operon, together with dhbE, a functional homolog of the Escherichia coli entE gene. Homologs of the E. coli entD and Vibrio cholerae …

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Louisiana Agriculture Spring, 2003, Lsu Agcenter Apr 2003

Louisiana Agriculture Spring, 2003, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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Inhibition Of Heparanase Activity And Heparanase-Induced Angiogenesis By Suramin Analogues, Dario Marchetti, Jane Reiland, Brad Erwin, Madhuchhanda Roy Mar 2003

Inhibition Of Heparanase Activity And Heparanase-Induced Angiogenesis By Suramin Analogues, Dario Marchetti, Jane Reiland, Brad Erwin, Madhuchhanda Roy

Faculty Publications

Heparanase, a heparan sulfate-specific endo-β-D-glucuronidase, plays an important role in tumor cell metastasis through the degradation of extracellular matrix heparan sulfate proteoglycans (ECM HSPG). Heparanase activity correlates with the metastatic propensity of tumor cells. Suramin, a polysulfonated naphthylurea, is an inhibitor of heparanase with suramin analogues shown to possess antiangiogenic and antiproliferative properties. We investigated the effects of selected suramin analogues (NF 127, NF 145 and NF 171) on heparanase activity and heparanase-driven angiogenesis. Studies of the ability of cellular extracts and purified heparanase from human, highly invasive and brain-metastatic melanoma (70W) cells revealed that heparanase expressed by these cells …

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A Causative Relationship Exists Between Eosinophils And The Development Of Allergic Pulmonary Pathologies In The Mouse, Hua Hao H. Shen, Sergei I. Ochkur, Michael P. Mcgarry, Jeffrey R. Crosby, Edie M. Hines, Michael T. Borchers, Huiying Wang, Travis L. Biechelle, Katie R. O'Neill, Tracy L. Ansay, Dana C. Colbert, Stephania A. Cormier, J. Paul Justice, Nancy A. Lee, James J. Lee Mar 2003

A Causative Relationship Exists Between Eosinophils And The Development Of Allergic Pulmonary Pathologies In The Mouse, Hua Hao H. Shen, Sergei I. Ochkur, Michael P. Mcgarry, Jeffrey R. Crosby, Edie M. Hines, Michael T. Borchers, Huiying Wang, Travis L. Biechelle, Katie R. O'Neill, Tracy L. Ansay, Dana C. Colbert, Stephania A. Cormier, J. Paul Justice, Nancy A. Lee, James J. Lee

Faculty Publications

Asthma and mouse models of allergic respiratory inflammation are invariably associated with a pulmonary eosinophilia; however, this association has remained correlative. In this report, a causative relationship between eosinophils and allergen-provoked pathologies was established using eosinophil adoptive transfer. Eosinophils were transferred directly into the lungs of either naive or OVA-treated IL-5-/- mice. This strategy resulted in a pulmonary eosinophilia equivalent to that observed in OVA-treated wild-type animals. A concomitant consequence of this eosinophil transfer was an increase in Th2 bronchoalveolar lavage cytokine levels and the restoration of intracellular epithelial mucus in OVA-treated IL-5-/- mice equivalent to OVA-treated wild-type levels. Moreover, …

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Bacillus Anthracis Virulence In Guinea Pigs Vaccinated With Anthrax Vaccine Adsorbed Is Linked To Plasmid Quantities And Clonality, Pamala R. Coker, Kimothy L. Smith, Patricia F. Fellows, Galena Rybachuck, Konstantin G. Kousoulas, Martin E. Hugh-Jones Mar 2003

Bacillus Anthracis Virulence In Guinea Pigs Vaccinated With Anthrax Vaccine Adsorbed Is Linked To Plasmid Quantities And Clonality, Pamala R. Coker, Kimothy L. Smith, Patricia F. Fellows, Galena Rybachuck, Konstantin G. Kousoulas, Martin E. Hugh-Jones

Faculty Publications

Bacillus anthracis is a bacterial pathogen of great importance, both historically and in the present. This study presents data collected from several investigations and indicates that B. anthracis virulence is associated with the clonality and virulence of plasmids pXO1 and pXO2. Guinea pigs vaccinated with Anthrax Vaccine Adsorbed were challenged with 20 B. anthracis isolates representative of worldwide genetic diversity. These same isolates were characterized with respect to plasmid copy number by using a novel method of quantitative PCR developed for rapid and efficient detection of B. anthracis from environmental samples. We found that the copy numbers for both pXO1 …

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Bioengineered ‘Golden’ Indica Rice Cultivars With Β-Carotene Metabolism In The Endosperm With Hygromycin And Mannose Selection Systems, Karabi Datta, Niranjan Baisakh, Norman Oliva, Lina Torrizo, Editha Abrigo, Jing Tan, Mayank Rai, Sayda Rehana, Salim Al-Babili, Peter Beyer, Ingo Potrykus, Swapan K. Datta Mar 2003

Bioengineered ‘Golden’ Indica Rice Cultivars With Β-Carotene Metabolism In The Endosperm With Hygromycin And Mannose Selection Systems, Karabi Datta, Niranjan Baisakh, Norman Oliva, Lina Torrizo, Editha Abrigo, Jing Tan, Mayank Rai, Sayda Rehana, Salim Al-Babili, Peter Beyer, Ingo Potrykus, Swapan K. Datta

Faculty Publications

Vitamin-A deficiency (VAD) is a major malnutrition problem in South Asia, where indica rice is the staple food. Indica-type rice varieties feed more than 2 billion people. Hence, we introduced a combination of transgenes using the biolistic system of transformation enabling biosynthesis of provitamin A in the endosperm of several indica rice cultivars adapted to diverse ecosystems of different countries. The rice seed-specific glutelin promoter (Gt-1 P) was used to drive the expression of phytoene synthase (psy), while lycopene β-cyclase (lcy) and phytoene desaturase (crtI), fused to the transit peptide sequence of the pea-Rubisco small subunit, were driven by the …

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Stat5a Promotes Adipogenesis In Nonprecursor Cells And Associates With The Glucocorticoid Receptor During Adipocyte Differentiation, Z. Elizabeth Floyd, Jacqueline M. Stephens Feb 2003

Stat5a Promotes Adipogenesis In Nonprecursor Cells And Associates With The Glucocorticoid Receptor During Adipocyte Differentiation, Z. Elizabeth Floyd, Jacqueline M. Stephens

Faculty Publications

The differentiation of adipocytes is regulated by the activity of a variety of transcription factors, including peroxidase proliferator-activated receptor (PPAR)-γ and C/EBPα. Our current study demonstrates that ectopic expression of STAT5A, such as that of PPAR-γ and C/EBPα, promotes adipogenesis in two nonprecursor fibroblast cell lines. Using morphologic and biochemical criteria, we have demonstrated that STAT5A and the combination of STAT5A and STAT5B are sufficient to induce the expression of early and late adipogenic markers in BALB/c and NIH-3T3 cells. Yet, the ectopic expression of STAT5B alone does not induce the expression of adipocyte genes, but enhances the induction of …

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Methylation-Sensitive Binding Of Transcription Factor Yy1 To An Insulator Sequence Within The Paternally Expressed Imprinted Gene, Peg3, Joomyeong Kim, Angela Kollhoff, Anne Bergmann, Lisa Stubbs Feb 2003

Methylation-Sensitive Binding Of Transcription Factor Yy1 To An Insulator Sequence Within The Paternally Expressed Imprinted Gene, Peg3, Joomyeong Kim, Angela Kollhoff, Anne Bergmann, Lisa Stubbs

Faculty Publications

The 5�-ends of two paternally expressed mouse genes, Peg3 and Usp29, are jointly associated with a CpG island that exhibits allele-specific methylation. Sequence comparison of the regions derived from human, mouse and cow revealed the presence of two evolutionarily conserved sequence motifs including one that is repeated multiple times within the first intron of Peg3 in all three mammals. DNA mobility shift and chromatin immunoprecipitation (ChlP) assays clearly demonstrated that this motif is an in vivo binding site for the Gli-type transcription factor YY1. The YY1-binding site contains one CpG dinucleotide, and methylation of this CpG site abolishes the binding …

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Transcriptional Initiation Under Conditions Of Anoxia-Induced Quiescence In Mitochondria From Artemia Franciscana Embryos, Brian D. Eads, Steven C. Hand Feb 2003

Transcriptional Initiation Under Conditions Of Anoxia-Induced Quiescence In Mitochondria From Artemia Franciscana Embryos, Brian D. Eads, Steven C. Hand

Faculty Publications

In response to anoxia, embryos of the brine shrimp Artemia franciscana are able coordinately to downregulate metabolism to levels low enough to permit survival for several years at room temperature. In addition to dramatic decreases in free ATP levels and heat production, intracellular pH drops from 7.8 to 6.3 overnight. Use of isolated mitochondria to study transcriptional responses to anoxia offers several advantages: (1) the localized nature of transcript initiation, processing and degradation, all of which may be followed in organello; (2) the relatively simple cis- and trans-machinery involved and (3) the ability to provide relevant physiological treatments in vitro. …

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Localization And Characterization Of Phenamil-Sensitive Na+ Influx In Isolated Rainbow Trout Gill Epithelial Cells, Scott D. Reid, G. S. Hawkings, F. Galvez, G. G. Goss Feb 2003

Localization And Characterization Of Phenamil-Sensitive Na+ Influx In Isolated Rainbow Trout Gill Epithelial Cells, Scott D. Reid, G. S. Hawkings, F. Galvez, G. G. Goss

Faculty Publications

Percoll density-gradient separation, combined with peanut lectin agglutinin (PNA) binding and magnetic bead separation, was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each of the sub-populations was performed to determine which displayed acid-activated phenamil- and bafilomycin-sensitive Na+ uptake. Analysis of the mechanism(s) of 22Na+ influx was performed in control and acid-activated (addition of 10 mmoll-1 proprionic acid) cells using a variety of Na+ transport inhibitors (ouabain, phenamil, HOE-694 and bumetanide) and a V-type ATPase inhibitor (bafilomycin). We found that cells migrating to a 1.03-1.05 g ml-1 Percoll interface [pavement cells (PVCs)] possessed the lowest rates …

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A Meta-Analysis Of Genome-Wide Linkage Scans For Hypertension: The National Heart, Lung And Blood Institute Family Blood Pressure Program, Michael A. Province, Sharon L.R. Kardia, Koustubh Ranade, Dabeeru C. Rao, Bonnie A. Thiel, Richard S. Cooper, Neil Risch, Stephen T. Turner, David R. Cox, Steven C. Hunt, Alan B. Weder, Eric Boerwinkle Feb 2003

A Meta-Analysis Of Genome-Wide Linkage Scans For Hypertension: The National Heart, Lung And Blood Institute Family Blood Pressure Program, Michael A. Province, Sharon L.R. Kardia, Koustubh Ranade, Dabeeru C. Rao, Bonnie A. Thiel, Richard S. Cooper, Neil Risch, Stephen T. Turner, David R. Cox, Steven C. Hunt, Alan B. Weder, Eric Boerwinkle

Faculty Publications

Background: Four multicenter Networks (GenNet, GENOA, HyperGEN, SAPPHIRe) form the National Heart, Lung and Blood Institute Family Blood Pressure Program (FBPP), to search for hypertension/blood pressure (BP) genes. The networks used different family designs and targeted multiple ethnic groups, using standardized protocols and definitions. Linkage genome scans were done on samples within each network (N = 6245 relatives). Methods: The evidence was synthesized using meta-analysis. Results: Combining ethnic groups, no region reached LOD >2, but several small peaks were identified, including chromosome 2p where two other recent reports find hypertension linkage. Conclusions: No regions show uniformly large effects on BP/hypertension …

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Matrix Heterogeneity And Host-Parasitoid Interactions In Space, James T. Cronin Jan 2003

Matrix Heterogeneity And Host-Parasitoid Interactions In Space, James T. Cronin

Faculty Publications

In this study, I experimentally examined how the landscape matrix influenced the movement, oviposition behavior, and spatial distribution of Anagrus columbi, a common egg parasitoid of the planthopper Prokelisia crocea. Both species exist among discrete patches of prairie cordgrass (Spartina pectinata), the sole host plant of P. crocea. Based on out-planted cordgrass pots bearing host eggs (to assess parasitism) or sticky leaves (traps for adult A. columbi), I found that the distribution of adult female A. columbi and pattern of ovipositions within a cordgrass patch were strongly matrix dependent. Female densities were 59% lower on the edge than interior of …

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Matrix Composition Affects The Spatial Ecology Of A Prairie Planthopper, Kyle J. Haynes, James T. Cronin Jan 2003

Matrix Composition Affects The Spatial Ecology Of A Prairie Planthopper, Kyle J. Haynes, James T. Cronin

Faculty Publications

To date, there is a lack of well-controlled field experiments that disentangle the effects of the intervening matrix from other landscape variables (e.g., patch geography or quality) that might influence animal dispersal among patches. We performed a field experiment to investigate how the movement of a delphacid planthopper (Prokelisia crocea) among discrete patches of prairie cordgrass (Spartina pectinata) is affected by the composition of the matrix (mudflat, native nonhost grasses, and the introduced grass smooth brome [Bromus inermis]). Within each matrix type, marked planthoppers were released onto experimental cordgrass patches that were made identical in size, isolation, and host plant …

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Two Rna Binding Proteins, Hen4 And Hua1 Act In The Processing Of Agamous Pre-Mrna In Arabidopsis Thaliana, Yulan Cheng, Naohiro Kato, Wenming Wang, Junjie Li, Xuemei Chen Jan 2003

Two Rna Binding Proteins, Hen4 And Hua1 Act In The Processing Of Agamous Pre-Mrna In Arabidopsis Thaliana, Yulan Cheng, Naohiro Kato, Wenming Wang, Junjie Li, Xuemei Chen

Faculty Publications

AGAMOUS, a key player in floral morphogenesis, specifies reproductive organ identities and regulates the timely termination of stem cell fates in the floral meristem. Here, we report that strains carrying mutations in three genes, HUA1, HUA2, and HUA ENHANCER4 (HEN4), exhibit floral defects similar to those in agamous mutants: reproductive-to-perianth organ transformation and loss of floral determinacy. HEN4 codes for a K homology (KH) domain-containing, putative RNA binding protein that interacts with HUA1, a CCCH zinc finger RNA binding protein in the nucleus. We show that HUA1 binds AGAMOUS pre-mRNA in vitro and that HEN4, HUA1, and HUA2 act in …

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Natural Bovine Lentiviral Type 1 Infection In Holstein Dairy Cattle. I. Clinical, Serological, And Pathological Observations, T. G. Snider, B. F. Jenny, P. G. Hoyt, K. S. Coats, K. F. Graves, C. R. Cooper, R. W. Storts, D. G. Luther Jan 2003

Natural Bovine Lentiviral Type 1 Infection In Holstein Dairy Cattle. I. Clinical, Serological, And Pathological Observations, T. G. Snider, B. F. Jenny, P. G. Hoyt, K. S. Coats, K. F. Graves, C. R. Cooper, R. W. Storts, D. G. Luther

Faculty Publications

Clinical, serological, and pathological abnormalities observed in Holstein cows naturally infected with bovine lentivirus 1 bovine immunodeficiency virus (BIV) and other infections were progressive and most commonly associated with weight loss, lymphoid system deficiency, and behavioral changes. Clinical evidence of meningoencephalitis was dullness, stupor, and occasional head or nose pressing postures. The polymerase chain reactions associated the BIV provirus with the lesions in the central nervous system and lymphoid tissues. Multiple concurrent infections developed in retrovirally infected cows undergoing normal stresses associated with parturition and lactation. A major functional correlate of the lymphoreticular alterations was the development of multiple secondary …

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Allelic Heterogeneity In Line-1 Retrotransposition Activity, Sheila M. Lutz, Bethaney J. Vincent, Haig H. Kazazian, Mark A. Batzer, John V. Moran Jan 2003

Allelic Heterogeneity In Line-1 Retrotransposition Activity, Sheila M. Lutz, Bethaney J. Vincent, Haig H. Kazazian, Mark A. Batzer, John V. Moran

Faculty Publications

De novo LINE-1 (long interspersed element-1, or L1) retrotransposition events are responsible for ∼1/1,000 disease-causing mutations in humans. Previously, L1.2 was identified as the likely progenitor of a mutagenic insertion in the factor VIII gene in a patient with hemophilia A. It subsequently was shown to be one of a small number of active L1s in the human genome. Here, we demonstrate that L1.2 is present at an intermediate insertion allele frequency in worldwide human populations and that common alleles (L1.2A and L1.2B) exhibit an ∼16-fold difference in their ability to retrotranspose in cultured human HeLa cells. Chimera analysis revealed …

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Haplotypes In The Dystrophin Dna Segment Point To A Mosaic Origin Of Modern Human Diversity, Ewa Ziȩtkiewicz, Vania Yotova, Dominik Gehl, Tina Wambach, Isabel Arrieta, Mark Batzer, David E.C. Cole, Peter Hechtman, Feige Kaplan, David Modiano, Jean Paul Moisan, Roman Michalski, Damian Labuda Jan 2003

Haplotypes In The Dystrophin Dna Segment Point To A Mosaic Origin Of Modern Human Diversity, Ewa Ziȩtkiewicz, Vania Yotova, Dominik Gehl, Tina Wambach, Isabel Arrieta, Mark Batzer, David E.C. Cole, Peter Hechtman, Feige Kaplan, David Modiano, Jean Paul Moisan, Roman Michalski, Damian Labuda

Faculty Publications

Although Africa has played a central role in human evolutionary history, certain studies have suggested that not all contemporary human genetic diversity is of recent African origin. We investigated 35 simple polymorphic sites and one Tn microsatellite in an 8-kb segment of the dystrophin gene. We found 86 haplotypes in 1,343 chromosomes from around the world. Although a classical out-of-Africa topology was observed in trees based on the variant frequencies, the tree of haplotype sequences reveals three lineages accounting for present-day diversity. The proportion of new recombinants and the diversity of the Tn microsatellite were used to estimate the age …

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Effect Of Horn Fly (Haematobia Irritans) Control On Growth And Reproduction Of Beef Heifers, S. M. Derouen, L. D. Foil, A. J. Mackay, D. E. Franke, D. W. Sanson, W. E. Wyatt Jan 2003

Effect Of Horn Fly (Haematobia Irritans) Control On Growth And Reproduction Of Beef Heifers, S. M. Derouen, L. D. Foil, A. J. Mackay, D. E. Franke, D. W. Sanson, W. E. Wyatt

Faculty Publications

The influence of horn fly control with commercially available ear tags was studied on beef replacement heifers (n = 670) for growth and reproductive performance. The study was conducted at five sites in Louisiana over 3 yr. Heifers used were yearling replacement females that were exposed to fertile bulls during a limited spring breeding season that coincided with the horn fly season. In mid to late May of each year, heifers were randomly assigned to one of two horn fly treatments: untreated and treated for horn fly control. The trial continued each year until September or October at the end …

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