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Hijos De La Decadencia: Transgressive Representations Of Gender In The Works Of Emilia Pardo Bazán, Sarah Berard
Hijos De La Decadencia: Transgressive Representations Of Gender In The Works Of Emilia Pardo Bazán, Sarah Berard
LSU Master's Theses
This thesis explores the transgressive representations of gender in the works of Emilia Pardo Bazán. In her short story “Cuento primitivo” (1893) and her novels Memorias de un solterón (1896), La quimera (1905), and Dulce dueño (1911), the myths and images that surround the figures of New Woman, femme fatales, and dandies expose the fear fin de siècle Spanish society felt toward these models that did not conform to the gender stereotypes expected of them. Their straying from the established norm was seen as the symptom of decadence and the herald of the destruction of the race. Each of the …
Evaluation Of Methods For Suppressing Estrus And Ovulation In Mares: Sustained Release Injections Of Altrenogest Versus Deslorelin Acetate, Thomas J. Stevens
Evaluation Of Methods For Suppressing Estrus And Ovulation In Mares: Sustained Release Injections Of Altrenogest Versus Deslorelin Acetate, Thomas J. Stevens
LSU Master's Theses
Two experiments assessed the potential of altrenogest, an artificial progestogen, and deslorelin acetate, a gonadotropin-releasing hormone (GnRH) agonist, for suppressing estrus and ovulation in cyclic mares. In the first experiment, mares were administered a luteolytic dose of prostaglandin-F2á on d 6 of diestrus, and were then treated with 1 of 3 formulations of altrenogest in slow-release vehicle (6 mares/group): 1) Biorelease altrenogest LA150 (225 mg total as a 1.5 mL injection); 2) Biorelease altrenogest LA225 (225 mg total as a 1.0 mL injection); or 3) Biorelease altrenogest LA225 (450 mg total as a 2.0 mL injection). Six control mares received …
A Comparison Of Response-Contingent And Noncontingent Pairing In The Conditioning Of A Reinforcer, Sarah Joanne Miller
A Comparison Of Response-Contingent And Noncontingent Pairing In The Conditioning Of A Reinforcer, Sarah Joanne Miller
LSU Master's Theses
Two pairing procedures were compared in the conditioning of a secondary reinforcer. Pairings of a neutral stimulus and primary reinforcer were delivered either contingent upon a participant response or noncontingently (i.e., independent of a participant response). Maintenance of responding when responses resulted in the neutral stimulus alone was compared before and throughout pairing using progressive-ratio probes. Results yielded undifferentiated responding between the contingently-paired and noncontingently-paired stimuli and a stimulus never paired with reinforcement, indicating that no conditioning effect occurred. Potential factors in producing this lack of differentiation are discussed, including differences in methodology between this study and past studies.
Habitat Use, Movements, And Ecology Of Female Mottled Ducks On The Gulf Coast Of Louisiana And Texas, Bruce Edward Davis
Habitat Use, Movements, And Ecology Of Female Mottled Ducks On The Gulf Coast Of Louisiana And Texas, Bruce Edward Davis
LSU Doctoral Dissertations
Mottled ducks (Anas fulvigula) are the most abundant breeding waterfowl in the coastal marshes of Louisiana and Texas. Mottled ducks are non-migratory and heavily dependent on coastal marsh habitats; they must satisfy all of their annual resource needs from within the Gulf Coast region. Coastal marsh habitats are being rapidly lost or degraded in Louisiana and Texas. The hydrology of many coastal marsh habitats has been altered by anthropogenic activity and natural factors. Parameters related to Mottled Duck habitat use and movements in this altered environment are poorly understood, and managers need information on vital rates of Mottled Ducks in …
Koutango: Under Reported Arboviral Disease In West Africa, Jaime Matias De Araujo Lobo
Koutango: Under Reported Arboviral Disease In West Africa, Jaime Matias De Araujo Lobo
LSU Doctoral Dissertations
Arthropod-borne viruses (arboviruses) are among the most common agents of human febrile illnesses worldwide. As crucially important emerging pathogens, they have caused multiple, notable epidemics of human disease and unnoticed epizootics over recent decades. Despite the public health relevance, very little is known about the geographic distribution of the agents and vectors, relative impact, and risk factors associated to the arboviral infection in many regions of the world and in the tropics in particular. Presented in this dissertation is an experimental study that explores the serology screening of serum samples from 151 patients whom were diagnosed with undifferentiated febrile illness …
Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto
Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto
LSU Master's Theses
Laminitis is an often fatal condition in horses with few available and only moderately effective treatment options. Separation of laminar dermis and epidermis lead to rotation or ventral deviation of the third phalanx inside the hoof capsule. Despite being a modified skin, equine laminar tissue does not completely return to normal after laminitis. The hypothesis tested was that adult progenitor cells in the equine laminar tissue are irreversibly damaged by laminitis. A method to harvest and culture cells in vitro from the equine lamina was established; and progenitor cells from unaffected and laminitic hooves were characterized and compared. Laminar tissue …
Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila
Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila
LSU Master's Theses
Arrhythmogenic cardiomyopathy (AC), also known as arrhythmogenic right ventricular cardiomyopathy (ARVC), is a disease characterized by fatty or fibro-fatty myocardial replacement, predominantly in the right ventricle and to a lower extent the left ventricle. It is recognized as a disease affecting the cardiac intercalated disc. Clinically, it is associated with ventricular arrhythmias, although atrial arrhythmias and atrial histopathological changes characteristic of AC have occasionally been reported. The full extent of atrial involvement in AC has not been investigated. Therefore, we aimed to apply histopathology, immunochemical detection, immunolocalization and transmission electron microscopy (TEM) techniques to characterize the distribution of desmosomal and …
Visible Near-Infrared Diffuse Reflectance Spectroscopy And Portable X-Ray Fluorescence Spectroscopy For Rapid Compost Analysis, Amanda Lynne Mcwhirt
Visible Near-Infrared Diffuse Reflectance Spectroscopy And Portable X-Ray Fluorescence Spectroscopy For Rapid Compost Analysis, Amanda Lynne Mcwhirt
LSU Master's Theses
Quantitative and qualitative evaluation of compost is necessary in order to provide consumers with basic knowledge about the product’s composition, and to protect public health and the environment by preventing the spread of contaminated material. Current methods for analysis of basic compost properties give accurate results but are time consuming and require numerous laboratory procedures. This study evaluated the use of visible near-infrared diffuse reflectance spectroscopy (VisNIR DRS) for organic matter (OM) determination and field portable X-ray fluorescence (PXRF) spectroscopy for determination of elemental composition of composted materials. These technologies were evaluated as alternatives to standard laboratory methods for their …
Examination Of The Reliability And Validity Of A New Observation Measure For Autism Spectrum Disorders: The Autism Spectrum Disorder Observation For Children, Daniene Neal
LSU Doctoral Dissertations
With increasing attention being drawn to autism spectrum disorders (ASD), specifically with regard to early and accurate diagnosis and treatment, researchers and clinicians alike have placed emphasis on finding assessment tools that can aid in this goal. The purpose of this study is to examine the psychometric properties of a new observation measure for ASD, the Autism Spectrum Disorders-Observation for Children (ASD-OC). The ASD-OC was found to have good to excellent interrater reliability, and excellent internal consistency (ƒÑ = .96). As a result of these initial reliability analyses, nine items were removed from the scale. The resulting 45 item ASD-OC …
The Responsibility Of Clothing Slaves In The United States As Described In Slave Petitions, 1775 To 1867, Ryan Jerel Aldridge
The Responsibility Of Clothing Slaves In The United States As Described In Slave Petitions, 1775 To 1867, Ryan Jerel Aldridge
LSU Doctoral Dissertations
The content analysis of eighteenth and nineteenth century slave petitions identified slave dress terms and descriptors by type and frequency and a descriptive analysis of slave dress petitions revealed the following themes: the expense and burden attributed to clothing slaves, slave dress neglect, hiring contracts that included slave dress, the use of slave dress as a reflection of the slaveholders character, compensation for slave dress provided by an individual other than the slaveholder, the quality of slave dress, the distribution of slave dress, and slave dress terminology descriptors. The most frequent specific provision request for slave dress items consisted of …
Metabolic Downregulation During Diapause In Embryos Of Artemia Franciscana, Yuvraj Nanasaheb Patil
Metabolic Downregulation During Diapause In Embryos Of Artemia Franciscana, Yuvraj Nanasaheb Patil
LSU Doctoral Dissertations
Encysted embryos of Artemia franciscana undergo a dramatic respiratory depression upon release from the adult female as they enter a state of hypometabolism termed diapause. The mechanisms by which such a respiratory depression is achieved remain unexplained. Evidence presented here shows that strategic enzymes involved in trehalose catabolism are inhibited during diapause, namely trehalase, hexokinase, pyruvate kinase and pyruvate dehydrogenase. Trehalose is the sole source of fuel in the embryos of A. franciscana, and hence downregulation of trehalose catabolism results in severe limitation of metabolic fuel available to the embryo during diapause. Western blot data demonstrates that pyruvate dehydrogenase …
Geometrical And Electronic Structure Of Metal Oxide Nanoparticles On Silica And Alumina, Udaya Indike Rodrigo
Geometrical And Electronic Structure Of Metal Oxide Nanoparticles On Silica And Alumina, Udaya Indike Rodrigo
LSU Doctoral Dissertations
Recent studies demonstrated nanometer-scale combustion-generated nanoparticles cause demonstrable health effects. It is essential to understand the chemical characteristics of these nanoparticles. Since combustion-generated nanoparticles arise in complex mixtures, we employed a dendrimeric method to generate simpler, more reproducible nanoparticles, which were tethered to fumed silica and alumina to provide surrogates for fly ash. We collected XANES and EXAFS data, which are useful for characterizing disordered mixtures where the metal is a minor component. The effect of particle size on structural properties was studied with copper oxide nanoparticles on fumed silica. XANES data demonstrated copper atoms in the nanoparticles are present …
Red Swamp Crayfish Procambarus Clarkii In The Atchafalaya River Basin: Biotic And Abiotic Effects On Population Dynamics And Physiological Biomarkers Of Hypoxic Stress, Christopher Paul Bonvillain
Red Swamp Crayfish Procambarus Clarkii In The Atchafalaya River Basin: Biotic And Abiotic Effects On Population Dynamics And Physiological Biomarkers Of Hypoxic Stress, Christopher Paul Bonvillain
LSU Doctoral Dissertations
Crayfish harvested from the Atchafalaya River Basin (ARB) represent the majority of Louisiana wild crayfish landings. However, excluding water level influences, it is difficult to elucidate inter-annual harvest differences and intra-annual population variability among habitats. This research investigated ecological influences on population characteristics of red swamp crayfish Procambarus clarkii in the southeastern ARB as well as physiological biomarkers of hypoxic stress in P. clarkii. Biotic and abiotic effects on P. clarkii populations were examined throughout the 2008 and 2009 commercial crayfish seasons. P. clarkii catch per unit effort (CPUE) at sampling locations increased nearly 600% between sample years despite similar …
Development And Characterization Of A Murine Model Of Rickettsia Parkeri Rickettsiosis, Britton Grasperge
Development And Characterization Of A Murine Model Of Rickettsia Parkeri Rickettsiosis, Britton Grasperge
LSU Doctoral Dissertations
Rickettsia parkeri, a member of the spotted fever group of Rickettsia, is the agent of an emerging rickettsiosis in the southeastern United States and South America. Despite increased recognition of human cases, limited information is available regarding infection of invertebrate and vertebrate hosts for this emerging tick-borne disease. Towards development of a viable transmission model and to further characterize the pathology associated with R. parkeri infection, inbred mouse strains (A/J, Balb/C, C3H/HeJ, and C3H/HeN) were intravenously and intradermally inoculated with R. parkeri. The C3H/HeJ strain of mice were identified as the most susceptible to R. parkeri infection and were found …
Generation And Characterization Of High Co₂ Requiring Mutants In Chlamydomonas Reinhardtii, Yunbing Ma
Generation And Characterization Of High Co₂ Requiring Mutants In Chlamydomonas Reinhardtii, Yunbing Ma
LSU Doctoral Dissertations
Chlamydomonas reinhardtii (referred to as C. reinhardtii hereinafter) possesses a CO2 concentrating mechanism (CCM) that allows the alga to grow at low CO2 concentrations (100 ppm CO2 in air). This dissertation represents the results from the characterization of three CCM mutants, as well as generation of eleven more potential mutants deficient in the CCM. One common feature seen in photosynthetic organisms possessing a CCM is the tight packaging of Rubisco within the cell. In many eukaryotic algae, Rubisco is localized to the pyrenoid, an electron dense structure within the chloroplast. The first mutant characterized has a highly disorganized pyrenoid. The …
Climate Change Displacement And Global Governance: A Case Study Of Three Intergovernmental Organizations And The Conflict Between The Member States And Bureaucracy, Andrea C. S. Berringer
Climate Change Displacement And Global Governance: A Case Study Of Three Intergovernmental Organizations And The Conflict Between The Member States And Bureaucracy, Andrea C. S. Berringer
LSU Doctoral Dissertations
Climate change is a topic most often broached by environmental scientists and activists and its effects are discussed in terms of animal populations and atmospheric events. However, its direct effect on human life is yet to garner such attention. A changing climate will affect how people are able to use their environment, if at all. Sea level rise and desertification will force a shift in human habitation. How the world seeks to deal with this shift is yet to be seen. The global governance of climate change-induced displacement is currently at the stage of ad hoc development. Legal and conceptual …
Overexpression Of A Plasma Membrane Protein Gene, Sapmp3, From Spartina Alterniflora L. Enhances Salinity Tolerance In Rice (Oryza Sativa L.), Hanamareddy Biradar
Overexpression Of A Plasma Membrane Protein Gene, Sapmp3, From Spartina Alterniflora L. Enhances Salinity Tolerance In Rice (Oryza Sativa L.), Hanamareddy Biradar
LSU Doctoral Dissertations
Salinity continues to be a major abiotic stress limiting crop productivity. As rice is staple food for nearly half of the world population, improvement in its salt tolerance will have a major impact on global food security. Compared to rice and other field crops, halophytes have evolved special physiological mechanisms to withstand high salinity. The overall goal of this study was to characterize plasma membrane protein 3 genes, SaPMP3-2 and SaPMP3-1, from a halophyte, Spartina alterniflora L., and evaluate their potential in single gene as well as pyramided transgenic plants in combination with the vacuolar ATPase subunit c1 (SaVHAc1) gene …
"Faith Is Life": A Qualitative Study Of Christian Faith And Chinese Immigrant Families, Yaxin Lu
"Faith Is Life": A Qualitative Study Of Christian Faith And Chinese Immigrant Families, Yaxin Lu
LSU Doctoral Dissertations
Research on religion and family in the United States has increased in the last two decades. With the increasing immigrant population, studies on minorities including Chinese immigrant families are also important. Religious faith has significant influences on some Chinese immigrant families. The purpose of the study is to examine the relationship between Christianity and Chinese immigrant families. Qualitative in-depth interviews were conducted with twenty-two highly religious Chinese Christian couples in the Southern United States. Twenty-two open-ended questions were asked to understand their conversion processes and how the Christian faith influenced the participants’ marriage and family life. Most of the participants …
Soil Climate And Pedology Of The Transylvanian Plain, Romania, Beatrix Haggard
Soil Climate And Pedology Of The Transylvanian Plain, Romania, Beatrix Haggard
LSU Doctoral Dissertations
The Transylvanian Plain (TP) is a 395,000 ha region located in north-central Romania and is an area of agronomic importance in the region. The TP is characterized by hilly terrain, dissected by the Someş and Mureş Rivers. The terrain creates a unique situation when assessing pedology and soil temperature. Soils can change quickly across the landscape in the TP due to the terrain. To account for these differences, soil temperature was measured to predict soil temperatures as well as to evaluate growing conditions. Twenty stations were installed for a long-term temperature and pedology study. Pedons were described for morphological characterization …
Synthesis, Cellular Studies, And Computational Analysis Of Porphyrin And Bodipy Conjugates With Affinity For Epidermal Growth Factor Receptor, Alecia Michelle Mccall
Synthesis, Cellular Studies, And Computational Analysis Of Porphyrin And Bodipy Conjugates With Affinity For Epidermal Growth Factor Receptor, Alecia Michelle Mccall
LSU Doctoral Dissertations
Chapter 1 is a description of the basic fundamental concepts of porphyrins and BODIPYs, (including synthetic methodologies), a general mechanism of the processes of EGFR, and computational concepts that are elaborated upon in future chapters. Chapter 2 is a discussion solely on the porphyrins that were synthesized to be used as fluorophores for EGFR-targeted conjugates, both peptidic and non-peptidic. The porphyrins were primarily synthesized via the Lindsey method as well as by O-alkylation of tetrahydroxy porphyrins. Synthesis of PEG linkages are discussed as well through three methodologies. Chapter 3 is a presentation of the synthesis, characterization, cellular studies, and computational …
Mechanisms Of Urban Influence On Precipitation In The Southeastern United States: Precipitation Enhancement, Storm Bifurcation, And Synoptic Characteristics, Anna Marie Trevino
Mechanisms Of Urban Influence On Precipitation In The Southeastern United States: Precipitation Enhancement, Storm Bifurcation, And Synoptic Characteristics, Anna Marie Trevino
LSU Doctoral Dissertations
The continual growth of urban areas increasingly affects the environment on various spatial scales. Land cover changes, combined with decreasing vegetative cover and addition of atmospheric aerosols, potentially lead to growing urban heat islands that alter the local moisture fluxes directly or indirectly, which in turn play a role in precipitation initiation and development. Some studies suggest that a region of enhanced rainfall exists downwind of the main urban area and that frontal systems decelerate as they reach areas of high urban development. Six urban areas within the southeastern United States were examined for possible urban precipitation enhancement: Atlanta, Birmingham, …
The Functional Morphology Of The Intermandibulo-Cervical Envelope Of The American Alligator (Alligator Mississippiensis), Brooke Hopkins Dubansky
The Functional Morphology Of The Intermandibulo-Cervical Envelope Of The American Alligator (Alligator Mississippiensis), Brooke Hopkins Dubansky
LSU Doctoral Dissertations
Alligators appear to swallow prey items that are large relative to their head size. Therefore, the intermandibulo-cervical envelope (i.e., the skin, Fascia superficialis, and constrictor musculature) was expected to be expandable. The three main layers of the intermandibulo-cervical envelope expand and recoil in tandem, but through different mechanisms. In the skin, which consists of hard-cornified scales and soft-cornified interscale skin segments, only the latter are expandable. Therefore, the width and orientation of the interscale skin segments determine the extent and direction of expansion of the skin. Whereas the intermandibular skin region is very expandable and enables the manipulation and crushing …
How Core Symptoms Of Autism Spectrum Disorder Predict Engagement In Specific Topographies Of Challenging Behavior, Sara Mahan
LSU Doctoral Dissertations
Challenging behavior, such as aggression, destructive behavior, and self-injurious behavior (SIB), are common among people of all ages with Autism Spectrum Disorder (ASD). Numerous researchers have found that greater severity of ASD or a diagnosis of ASD is significantly correlated with greater levels of challenging behavior. However, there is dearth of information on how core symptoms of ASD (i.e., socialization deficits, communication deficits, stereotypies) predict the engagement of specific topographies of challenging behavior above and beyond other variables, such as developmental functioning. The purpose of this study is to extend the current literature base through examining how core symptoms of …
Constructing Wilderness: The Nexus Of Preservation And Ocean-Space In The United States, Ryan Orgera
Constructing Wilderness: The Nexus Of Preservation And Ocean-Space In The United States, Ryan Orgera
LSU Doctoral Dissertations
The ocean has long played a minor role in human geography; imagining it as natural space rather than an extractive space even less significant. This dissertation explores the most revered kind of American nature preservation: wilderness. Despite the millions of acres set aside as wilderness in the United States, no such designation exists for ocean-space as a discrete entity. Through the analysis of congressional hearings, bills, resolutions, public laws, and maps, this dissertation uncovers the complex constructs of the production of legal wilderness. Furthermore, it uncovers a novel vein of inquiry, that of the ocean as a preserved natural space. …
Effects Of Fire And Treefalls On Japanese Climbing Fern And Native Species Groundcover In A Restored Longleaf Pine Savanna, Becky Jolene Carmichael
Effects Of Fire And Treefalls On Japanese Climbing Fern And Native Species Groundcover In A Restored Longleaf Pine Savanna, Becky Jolene Carmichael
LSU Doctoral Dissertations
Recurrent disturbances play an important role in maintaining longleaf pine savannas. Windstorms and fires contribute to the heterogeneity of pine savanna understory through generation of canopy gaps and fuel accumulation, distribution and consumption. The combination of windstorms and fires promotes native herbaceous species, but also promotes invasion of Japanese climbing fern (Lygodium japonicum). Lygodium japonicum is an invasive fern with subterranean rhizomes and copious spore production invading pine savannas along the southeastern Gulf Coast Region. This dissertation examines how fire, previous windstorm disturbances, and animal disruptions influence L. japonicum and native species abundance in a restored longleaf pine savanna. Studies …
Energy, Environment, And Sustainability: A Hierarchical Analysis Of South Louisiana, Matthew Korbel Moerschbaecher
Energy, Environment, And Sustainability: A Hierarchical Analysis Of South Louisiana, Matthew Korbel Moerschbaecher
LSU Doctoral Dissertations
This dissertation details research into the sustainability of industrial, human, and natural systems in south Louisiana. Chapter 1 is a general introduction. Chapter 2 calculates the energy return on financial investment (EROFI) of oil and gas production in the ultra-deepwater Gulf of Mexico (GoM) in 2009 and the Macondo Prospect (Mississippi Canyon Block 252). I calculated a preliminary Energy Return on Investment (EROI) using a range of energy intensity ratios. The EROFI for ultra-deepwater oil and gas at the wellhead was roughly 0.85 gallons, per dollar. These estimates of EROI for 2009 ultra-deepwater oil and natural gas at the wellhead …
High-Throughput Sperm Cryopreservation Of Aquatic Species, E Hu
High-Throughput Sperm Cryopreservation Of Aquatic Species, E Hu
LSU Doctoral Dissertations
The goal of this dissertation was to integrate multiple disciplines for application of high-throughput sperm cryopreservation to aquatic species. The blue catfish Ictalurus furcatus was selected for research due to its role in production of hybrid catfish with eggs from channel catfish Ictalurus punctatus. A high-throughput cryopreservation pathway was developed by systematically evaluating each process factor and using automated processing systems. Large quantities of cryopreserved sperm produced from this protocol were evaluated in commercial-scale hatcheries in cooperation with aquaculture producers. To develop production for commercial requirements, quality characteristics of materials and products were identified and recorded during processing and analyzed …
Mechanisms By Which Exercise Training Attenuates Blood Pressure In Animals: Roles Of Cytokines, Oxidative Stress, And Glycogen Synthase Kinase-3Â, Deepmala Agarwal
Mechanisms By Which Exercise Training Attenuates Blood Pressure In Animals: Roles Of Cytokines, Oxidative Stress, And Glycogen Synthase Kinase-3Â, Deepmala Agarwal
LSU Doctoral Dissertations
Hypertension is a chronic multifactorial condition with high morbidity and mortality rates, currently affecting about one billion people worldwide. Currently available anti-hypertensive medications are found to be effective in reducing blood pressure (BP), but still more than 50% of those diagnosed with hypertension fail to respond to these anti-hypertensive regimens. Although hypertension has multiple etiologies, physical inactivity has been found to have strong correlation with the disease, so exercise has recently been recommended as a part of lifestyle modifications for all hypertensive patients. Therefore, the present series of in vivo and in vitro studies were undertaken to gain more insight …
The Solo String Works Of J. S. Bach: The Relationship Between Dance And Musical Elements, Chung-Hui Hsu
The Solo String Works Of J. S. Bach: The Relationship Between Dance And Musical Elements, Chung-Hui Hsu
LSU Doctoral Dissertations
In 1685, the Doge of Genoa made a visit to the French court and asked Louis XIV to host a ball. Louis XIV responded affirmatively and arranged a magnificent dance in his private apartment. The type of dance that took place was a kind of social dancing which later became the standard included Allemande, Courante, Sarabande, Gigue, Minuet, Gavotte, Bourée, Loure and Chaconne. These dances were called theatrical dances when they were used in theatrical production by professional dancers. During this period, the relationship between composer and choreographer was sometimes inseparable. Maestro Jean-Baptiste Lully (1632-1687) and his well-known choreographer Pierre …
Single-Molecule Detection Of Molecular Beacons Generated From Ldr On Thermoplastic Microfluidic Device For Bioanalysis, Zhiyong Peng
Single-Molecule Detection Of Molecular Beacons Generated From Ldr On Thermoplastic Microfluidic Device For Bioanalysis, Zhiyong Peng
LSU Doctoral Dissertations
Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications. This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal the presence of certain targets using reporter sequence found in their genome without requiring PCR. This assay coupled the sensitivity of single-pair fluorescence resonance energy transfer (spFRET) with the specificity of ligase detection reaction (LDR) to provide near real-time readout of target biomarkers. Heightened concerns on potential bioterrorism threats, such as rapid dissemination of pathogenic bacteria or viruses into …