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Louisiana Agriculture Spring, 2016 Apr 2016

Louisiana Agriculture Spring, 2016

Louisiana Agriculture

No abstract provided.

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Laser Ablation Sample Transfer For Localized Lc-Ms/Ms Proteomic Analysis Of Tissue, Fabrizio Donnarumma, Kermit K. Murray Apr 2016

Laser Ablation Sample Transfer For Localized Lc-Ms/Ms Proteomic Analysis Of Tissue, Fabrizio Donnarumma, Kermit K. Murray

Faculty Publications

© 2016 John Wiley & Sons, Ltd. We have developed a mid-infrared laser ablation sampling technique for nano-flow liquid chromatography coupled with tandem mass spectrometry proteomic profiling of discrete regions from biological samples. Laser ablation performed in transmission geometry was used to transfer material from 50-μm thick tissue sections mounted on a glass microscope slide to a capturing solvent. Captured samples were processed using filter-aided sample preparation and enzymatically digested to produce tryptic peptides for data-dependent analysis with an ion trap mass spectrometer. Comparison with ultraviolet laser capture microdissection from neighboring regions on the same tissue section revealed that infrared …

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Growth Dynamics Of A Laminated Microbial Mat In Response To Variable Irradiance In An Antarctic Lake, Ian Hawes, Anne D. Jungblut, Maciej K. Obryk, Peter T. Doran Apr 2016

Growth Dynamics Of A Laminated Microbial Mat In Response To Variable Irradiance In An Antarctic Lake, Ian Hawes, Anne D. Jungblut, Maciej K. Obryk, Peter T. Doran

Faculty Publications

© 2016 John Wiley & Sons Ltd. Laminated microbial mats are important ecosystem components of perennially ice-covered Antarctic dry valley lakes. In order to understand better their response to changing environment, we made observations and carried out a manipulation experiment to determine their response to variations in irradiance in Lake Hoare (77°38′ S, 162°53′ E). Ice transparency was the most variable parameter that affected benthic light dose, both spatially and between years. Patterns of lamina accrual corresponded to irradiance history, with laminae that were initiated in high transmission years thicker than those from low transmission years. A shading experiment confirmed …

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14th Congress Of Combustion By-Products And Their Health Effects—Origin, Fate, And Health Effects Of Combustion-Related Air Pollutants In The Coming Era Of Bio-Based Energy Sources, Eva Weidemann, Patrik L. Andersson, Terry Bidleman, Christoffer Boman, Danielle J. Carlin, Elena Collina, Stephania A. Cormier, Sandra C. Gouveia-Figueira, Brian K. Gullett, Christer Johansson, Donald Lucas, Lisa Lundin, Staffan Lundstedt, Stellan Marklund, Malin L. Nording, Nuria Ortuño, Asmaa A. Sallam, Florian M. Schmidt, Stina Jansson Apr 2016

14th Congress Of Combustion By-Products And Their Health Effects—Origin, Fate, And Health Effects Of Combustion-Related Air Pollutants In The Coming Era Of Bio-Based Energy Sources, Eva Weidemann, Patrik L. Andersson, Terry Bidleman, Christoffer Boman, Danielle J. Carlin, Elena Collina, Stephania A. Cormier, Sandra C. Gouveia-Figueira, Brian K. Gullett, Christer Johansson, Donald Lucas, Lisa Lundin, Staffan Lundstedt, Stellan Marklund, Malin L. Nording, Nuria Ortuño, Asmaa A. Sallam, Florian M. Schmidt, Stina Jansson

Faculty Publications

© 2016, Springer-Verlag Berlin Heidelberg. The 14th International Congress on Combustion By-Products and Their Health Effects was held in Umeå, Sweden from June 14th to 17th, 2015. The Congress, mainly sponsored by the National Institute of Environmental Health Sciences Superfund Research Program and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, focused on the “Origin, fate and health effects of combustion-related air pollutants in the coming era of bio-based energy sources”. The international delegates included academic and government researchers, engineers, scientists, policymakers and representatives of industrial partners. The Congress provided a unique forum for the discussion of …

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Phylogenetic And Epigenetic Footprinting Of The Putative Enhancers Of The Peg3 Domain, Joomyeong Kim, An Ye Apr 2016

Phylogenetic And Epigenetic Footprinting Of The Putative Enhancers Of The Peg3 Domain, Joomyeong Kim, An Ye

Faculty Publications

© 2016 Kim, Ye. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Peg3 (Paternally Expressed Gene 3) imprinted domain is predicted to be regulated through a large number of evolutionarily conserved regions (ECRs) that are localized within its middle 200-kb region. In the current study, we characterized these potential cis-regulatory regions using phylogenetic and epigenetic approaches. According to the results, the majority of these ECRs are potential enhancers for the transcription of the …

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Revisiting Coptotermes (Isoptera: Rhinotermitidae): A Global Taxonomic Road Map For Species Validity And Distribution Of An Economically Important Subterranean Termite Genus, Thomas Chouvenc, Hou Feng Li, James Austin, Christian Bordereau, Thomas Bourguignon, Stephen L. Cameron, Eliana M. Cancello, Reginaldo Constantino, Ana Maria Costa-Leonardo, Paul Eggleton, Theodore A. Evans, Brian Forschler, J. Kenneth Grace, Claudia Husseneder, Jan Křeček, Chow Yang Lee, Timothy Lee, Nathan Lo, Matthew Messenger, Aaron Mullins, Alain Robert, Yves Roisin, Rudolf H. Scheffrahn, David Sillam-Dussès, Jan Šobotník, Allen Szalanski, Yoko Takematsu, Edward L. Vargo, Akinori Yamada, Tsuyoshi Yoshimura, Nan Yao Su Apr 2016

Revisiting Coptotermes (Isoptera: Rhinotermitidae): A Global Taxonomic Road Map For Species Validity And Distribution Of An Economically Important Subterranean Termite Genus, Thomas Chouvenc, Hou Feng Li, James Austin, Christian Bordereau, Thomas Bourguignon, Stephen L. Cameron, Eliana M. Cancello, Reginaldo Constantino, Ana Maria Costa-Leonardo, Paul Eggleton, Theodore A. Evans, Brian Forschler, J. Kenneth Grace, Claudia Husseneder, Jan Křeček, Chow Yang Lee, Timothy Lee, Nathan Lo, Matthew Messenger, Aaron Mullins, Alain Robert, Yves Roisin, Rudolf H. Scheffrahn, David Sillam-Dussès, Jan Šobotník, Allen Szalanski, Yoko Takematsu, Edward L. Vargo, Akinori Yamada, Tsuyoshi Yoshimura, Nan Yao Su

Faculty Publications

No abstract provided.

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The Effects Of Mitochondrial Dna On The Fitness Of The Marine Crustacean Tigripus Californicus, Ellen Foster Apr 2016

The Effects Of Mitochondrial Dna On The Fitness Of The Marine Crustacean Tigripus Californicus, Ellen Foster

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No abstract provided.

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Can Parasitized Lambs Self-Medicate Through Preferred Consumption Of Sericea Lespedeza Pellets?, Mallory Alicia Garcia Apr 2016

Can Parasitized Lambs Self-Medicate Through Preferred Consumption Of Sericea Lespedeza Pellets?, Mallory Alicia Garcia

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No abstract provided.

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Development Of Mouse Melanoma Model To Evaluate Oncolytic Viral Therapy Using Osv, Kelli A. Clemons Apr 2016

Development Of Mouse Melanoma Model To Evaluate Oncolytic Viral Therapy Using Osv, Kelli A. Clemons

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Compass: A Musical Journey Through Nature, Matthew Depaula Apr 2016

Compass: A Musical Journey Through Nature, Matthew Depaula

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No abstract provided.

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“My Way Or The Highway To Heaven”: The Role Of Choice In Religious Conversion, Marylynn Smitherman Apr 2016

“My Way Or The Highway To Heaven”: The Role Of Choice In Religious Conversion, Marylynn Smitherman

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Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer Apr 2016

Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer

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No abstract provided.

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Determination Of Sperm Concentration Using Flow Cytometry With Simultaneous Analysis Of Sperm Plasma Membrane Integrity In Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Terrence R. Tiersch Apr 2016

Determination Of Sperm Concentration Using Flow Cytometry With Simultaneous Analysis Of Sperm Plasma Membrane Integrity In Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Terrence R. Tiersch

Faculty Publications

Control of sperm concentration is required to ensure consistent and reproducible results for cryopreservation and in vitro fertilization protocols. Determination of sperm concentration is traditionally performed with a counting chamber (e.g., hemocytometer), or more recently with a spectrophotometer. For small-sized biomedical model fishes, the availability of sperm sample is limited to microliters, so it is desirable to develop fast and accurate approaches for concentration determination that also minimize sample use. In this study, a new approach was developed for sperm concentration determination using a flow cytometer (Accuri C6, BD Biosciences, San Jose, CA) with simultaneous measurement of sperm membrane integrity …

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A Procedure-Spanning Analysis Of Plasma Membrane Integrity For Assessment Of Cell Viability In Sperm Cryopreservation Of Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Carrie Carmichael, Jen Matthews, Zoltan M. Varga, Terrence Tiersch Apr 2016

A Procedure-Spanning Analysis Of Plasma Membrane Integrity For Assessment Of Cell Viability In Sperm Cryopreservation Of Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Carrie Carmichael, Jen Matthews, Zoltan M. Varga, Terrence Tiersch

Faculty Publications

The goal of this study was to evaluate plasma membrane integrity and motility for zebrafish sperm quality assessment along the cryopreservation pathway - from sample collection through refrigerated storage, cryoprotectant equilibration, freezing, thawing, and fertilization. The objectives were to: (1) evaluate the effects of osmolality, extender, and refrigerated storage on sperm plasma membrane integrity and motility, and (2) compare cryopreservation of sperm from farm-raised and well-characterized research populations by evaluating motility and membrane integrity of fresh, post-equilibration (before freezing) and post-thaw sperm, and post-thaw fertility. Osmolality, extender, and storage time each influenced sperm motility and membrane integrity. Isotonic osmolality showed …

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Climate, Habitat Associations And The Potential Distributions Of Neotropical Birds: Implications For Diversification Across The Andes, Carlos Daniel Cadena, Carlos A. Pedraza, Robb T. Brumfield Apr 2016

Climate, Habitat Associations And The Potential Distributions Of Neotropical Birds: Implications For Diversification Across The Andes, Carlos Daniel Cadena, Carlos A. Pedraza, Robb T. Brumfield

Faculty Publications

Contrary to the long-held view that the uplift of the Andes spurred biotic diversification by causing vicariance across multiple lineages with formerly continuous distributions, recent work suggests that dispersal across the Andes occurring after major uplift episodes was a major driver of speciation in Neotropical birds, with the ability of lineages to persist and disperse in the landscape being strong predictors of speciation. However, where and when dispersal events across the Andes occurred is unknown, and the role of climatic fluctuations and associated shifts in vegetation in promoting cross-Andes dispersal at different moments remains to be studied. We used models …

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The Potential Role Of Polyamines In Gill Epithelial Remodeling During Extreme Hypoosmotic Challenges In The Gulf Killifish, Fundulus Grandis, Ying Guan, Guo Xia Zhang, Shujun Zhang, Beau Domangue, Fernando Galvez Apr 2016

The Potential Role Of Polyamines In Gill Epithelial Remodeling During Extreme Hypoosmotic Challenges In The Gulf Killifish, Fundulus Grandis, Ying Guan, Guo Xia Zhang, Shujun Zhang, Beau Domangue, Fernando Galvez

Faculty Publications

© 2016 Elsevier Inc. Polyamines are a family of low molecular weight organic cations produced in part by the coordinated actions of arginase II (Arg II) and ornithine decarboxylase (Odc). Although gill polyamine homeostasis is affected by acute transfer to fresh water, little is known of its function in fish osmoregulation. The current study investigated the role of polyamines in the compensatory response of hypoosmotic challenge in the euryhaline fish, Fundulus grandis. Adult F. grandis were acclimated to 5ppt water, transferred abruptly to 5, 2, 1, 0.5 and 0.1 ppt water, and assessed for osmoregulatory function, gill morphology, and polyamine …

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Environmental Stress In The Gulf Of Mexico And Its Potential Impact On Public Health, B. Singleton, J. Turner, L. Walter, N. Lathan, D. Thorpe, P. Ogbevoen, J. Daye, D. Alcorn, S. Wilson, J. Semien, T. Richard, T. Johnson, K. Mccabe, J. J. Estrada, F. Galvez, C. Velasco, K. Reiss Apr 2016

Environmental Stress In The Gulf Of Mexico And Its Potential Impact On Public Health, B. Singleton, J. Turner, L. Walter, N. Lathan, D. Thorpe, P. Ogbevoen, J. Daye, D. Alcorn, S. Wilson, J. Semien, T. Richard, T. Johnson, K. Mccabe, J. J. Estrada, F. Galvez, C. Velasco, K. Reiss

Faculty Publications

© 2015 Elsevier Inc. The Deepwater Horizon (DWH) oil spill in the Gulf of Mexico was the largest maritime oil spill in history resulting in the accumulation of genotoxic substances in the air, soil, and water. This has potential far-reaching health impacts on cleanup field workers and on the populations living in the contaminated coastal areas. We have employed portable airborne particulate matter samplers (SKC Biosampler Impinger) and a genetically engineered bacterial reporter system (umu-ChromoTest from EBPI) to determine levels of genotoxicity of air samples collected from highly contaminated areas of coastal Louisiana including Grand Isle, Port Fourchon, and Elmer's …

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The Effect Of Emotional Words And Sounds In Multidimensional Source Memory, Katelyn M. Mccoy Apr 2016

The Effect Of Emotional Words And Sounds In Multidimensional Source Memory, Katelyn M. Mccoy

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No abstract provided.

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Road Associated Wildlife Mortality In An Urbanizing Metropolitan Area Of The Southeastern Usa, Lillian Miller Apr 2016

Road Associated Wildlife Mortality In An Urbanizing Metropolitan Area Of The Southeastern Usa, Lillian Miller

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No abstract provided.

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Development Of A Transposon-Based Vector To Express Large Quantities Of Factor Viii, Allison Mikes Apr 2016

Development Of A Transposon-Based Vector To Express Large Quantities Of Factor Viii, Allison Mikes

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No abstract provided.

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Faunal Analysis Of Fauna In Feature 1, Byrd Hammock South Site, Wakulla County, Fl, Jacob Mendoza Apr 2016

Faunal Analysis Of Fauna In Feature 1, Byrd Hammock South Site, Wakulla County, Fl, Jacob Mendoza

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No abstract provided.

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Zein Nanoparticle Interaction With Soybean Plants, Kurt Ristroph Apr 2016

Zein Nanoparticle Interaction With Soybean Plants, Kurt Ristroph

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No abstract provided.

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15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler Apr 2016

15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler

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No abstract provided.

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Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker Apr 2016

Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker

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No abstract provided.

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The Rat Kingdom, Laura Gauggel Apr 2016

The Rat Kingdom, Laura Gauggel

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No abstract provided.

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Molecular Markers Located On The Dgat1, Cast, And Lepr Genes And Their Associations With Milk Production And Fertility Traits In Holstein Cattle, R. Hill, A. Canal, K. Bondioli, R. Morell, M. D. Garcia Mar 2016

Molecular Markers Located On The Dgat1, Cast, And Lepr Genes And Their Associations With Milk Production And Fertility Traits In Holstein Cattle, R. Hill, A. Canal, K. Bondioli, R. Morell, M. D. Garcia

Faculty Publications

© FUNPEC-RP. The objective of the present study was to investigate single nucleotide polymorphisms (SNPs) located in three candidate genes previously reported to have effects on fertility and milk production traits in a population of 123 Holstein cows. The milk production traits evaluated included lifetime averages of milk yield, protein concentration, and fat concentration. Fertility traits evaluated included lifetime averages of services per conception and days-open. Candidate genes included those encoding diacylglycerol acyltransferase (DGAT1), leptin receptor (LEPR), and calpastatin (CAST). A total of 60 SNPs were selected (20 per gene) at equidistant locations on each candidate gene to identify potential …

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Astrocytes Regulate Glp-1 Receptor-Mediated Effects On Energy Balance, David J. Reiner, Elizabeth G. Mietlicki-Baase, Lauren E. Mcgrath, Derek J. Zimmer, Kendra K. Bence, Gregory L. Sousa, Vaibhav R. Konanur, Joanna Krawczyk, David H. Burk, Scott E. Kanoski, Gerlinda E. Hermann, Richard C. Rogers, Matthew R. Hayes Mar 2016

Astrocytes Regulate Glp-1 Receptor-Mediated Effects On Energy Balance, David J. Reiner, Elizabeth G. Mietlicki-Baase, Lauren E. Mcgrath, Derek J. Zimmer, Kendra K. Bence, Gregory L. Sousa, Vaibhav R. Konanur, Joanna Krawczyk, David H. Burk, Scott E. Kanoski, Gerlinda E. Hermann, Richard C. Rogers, Matthew R. Hayes

Faculty Publications

© 2016 the authors. Astrocytes are well established modulators of extracellular glutamate, but their direct influence on energy balance-relevant behaviors is largely understudied. As the anorectic effects of glucagon-like peptide-1 receptor (GLP-1R) agonists are partly mediated by central modulation of glutamatergic signaling, we tested the hypothesis that astrocytic GLP-1R signaling regulates energy balance in rats. Central or peripheral administration of a fluorophore-labeled GLP-1R agonist, exendin-4, localizes within astrocytes and neurons in the nucleus tractus solitarius (NTS), a hindbrain nucleus critical for energy balance control. This effect is mediated by GLP-1R, as the uptake of systemically administered fluorophore-tagged exendin-4 was blocked …

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Pelvic Sexual Dimorphism Among Species Monomorphic In Body Size: Relationship To Relative Newborn Body Mass, Robert G. Tague Mar 2016

Pelvic Sexual Dimorphism Among Species Monomorphic In Body Size: Relationship To Relative Newborn Body Mass, Robert G. Tague

Faculty Publications

Females have larger pelves than males among eutherians to mitigate obstetrical difficulty. This study addresses 3 issues concerning pelvic sexual dimorphism using 8 species that are sexually monomorphic in nonpelvic size: Aotus azarae, Castor canadensis, Dasypus novemcinctus, Hylobates lar, Saguinus geoffroyi, Sciurus carolinensis, Sylvilagus floridanus, and Urocyon cinereoargenteus. Using published data to compute the index of relative newborn body mass (RNBM = [newborn body mass/adult female body mass]100%) for 266 eutherian species, A. azarae, H. lar, and S. geoffroyi are characterized as giving birth to relatively large newborns and the other 5 species as giving birth to relatively small newborns. …

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Dietary Plant Lectins Appear To Be Transported From The Gut To Gain Access To And Alter Dopaminergic Neurons Of Caenorhabditis Elegans, A Potential Etiology Of Parkinson’S Disease, Jolene Zheng, Mingming Wang, Wenqian Wei, Jeffrey N. Keller, Binita Adhikari, Jason F. King, Michael L. King, Nan Peng, Roger A. Laine Mar 2016

Dietary Plant Lectins Appear To Be Transported From The Gut To Gain Access To And Alter Dopaminergic Neurons Of Caenorhabditis Elegans, A Potential Etiology Of Parkinson’S Disease, Jolene Zheng, Mingming Wang, Wenqian Wei, Jeffrey N. Keller, Binita Adhikari, Jason F. King, Michael L. King, Nan Peng, Roger A. Laine

Faculty Publications

© Copyright © 2016 Zheng, Wang, Wei, Keller, Adhikari, King, King, Peng and Laine. Lectins from dietary plants have been shown to enhance drug absorption in the gastrointestinal tract of rats, be transported trans-synaptically as shown by tracing of axonal and dendritic paths, and enhance gene delivery. Other carbohydrate-binding protein toxins are known to traverse the gut intact in dogs. Post-feeding rhodamine- or TRITC-tagged dietary lectins, the lectins were tracked from gut to dopaminergic neurons (DAergic-N) in transgenic Caenorhabditis elegans (C. elegans) [egIs1(Pdat-1:GFP)] where the mutant has the green fluorescent protein (GFP) gene fused to a dopamine transport protein gene …

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Cucumis Melo Endornavirus: Genome Organization, Host Range And Co-Divergence With The Host, Sead Sabanadzovic, William M. Wintermantel, Rodrigo A. Valverde, James D. Mccreight, Nina Aboughanem-Sabanadzovic Mar 2016

Cucumis Melo Endornavirus: Genome Organization, Host Range And Co-Divergence With The Host, Sead Sabanadzovic, William M. Wintermantel, Rodrigo A. Valverde, James D. Mccreight, Nina Aboughanem-Sabanadzovic

Faculty Publications

A high molecular weight dsRNA was isolated from a Cucumis melo L. plant (referred to as 'CL01') of an unknown cultivar and completely sequenced. Sequence analyses showed that dsRNA is associated with an endornavirus for which a name Cucumis melo endornavirus (CmEV) is proposed. The genome of CmEV-CL01 consists of 15,078 nt, contains a single, 4939 codons-long ORF and terminates with a stretch of 10 cytosine residues. Comparisons of the putative CmEV-encoded polyprotein with available references in protein databases revealed a unique genome organization characterized by the presence of the following domains: viral helicase Superfamily 1 (Hel-1), three glucosyltransferases (doublet …

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