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Sexually Dimorphic Oxytocin Receptor-Expressing Neurons In The Anteroventral Periventricular Nucleus Regulates Maternal Behavior, Kaustubh Sharma

LSU Doctoral Dissertations

The neurohypophysial hormone oxytocin is involved in the regulation of social behaviors, including social recognition, pair bonding, and sex-specific parental behaviors in a variety of species. Oxytocin triggers these social behaviors by binding to oxytocin receptors (OXTR) in various parts of the brain. Oxytocin-induced sex-typical behavior, therefore, suggests a sexual dimorphic distribution of OXTR in the brain. In recent years, the oxytocin system in the brain received tremendous attention as a potential pharmacological target for treatment of many psychiatric disorders, such as anxiety, autism spectrum disorders, and even sex-specific psychiatric disorder like postpartum depression (PPD). An important problem and a …

Evolution Of Green Blood In New Guinea Lizards: Phylogenomics, Biogeography, And Comparative Genomics, Zachary Rodriguez

LSU Doctoral Dissertations

Non-model organisms with evolutionary novelties and complex distributions can provide valuable insight into the mechanisms underlying biological diversity. Green blood is one of the most unusual vertebrate physiologies and has repeatedly evolved in lizards from the megadiverse island of New Guinea. An unusually high concentration of the toxic green bile pigment biliverdin causes the green coloration of these lizards' blood, muscles, and bones. This dissertation uncovered the complex history of this novel trait (Chapter 2), identified protein-coding sequences that underlie green blood in lizards (Chapter 3), and explored evolutionary processes that drive genetic diversity in high-elevation lizards. To accurately trace …

Function, History, And Ecology In The Exceptional Radiation Of Murine Rodents, Jonathan Allen Nations

LSU Doctoral Dissertations

Life’s diversity is not spread evenly across all lineages, and this unevenness is thought to be due, in part, to a few interwoven factors: biogeographic history, the evolution of successful functional traits, and the ecological opportunity these traits afford. My dissertation focuses on the evolution of a species-rich and morphologically diverse clade of mammals, the murine rodents (Murinae; Muridae; Rodentia) to address 1) morphological adaptations associated with niche transition to arboreality 2) the effect of repeated ecological transitions on murine diversification, and 3) the role of atypical ecological niches in the assembly of hyperdiverse communities. My dissertation has revealed that …

Incorporation Of Lignin In Natural And Synthetic Biomaterials To Alter Mechanical And Biochemical Properties For Enhanced Wound Healing, Jorge Alfonso Belgodere

LSU Doctoral Dissertations

It is estimated that chronic, non-healing wounds affect more than 6.5 million Americans annually, with an estimated healthcare cost beyond $14 billion. Here, we attempted to create composites of natural (collagen type I or gelatin-methacrylate) or synthetic (poly(ethylene glycol) polymers incorporating a natural plant component, lignin, to combat the costs and limitations current wound healing methods face. Three-dimensional matrices of collagen type I (Col I) are widely used in tissue engineering applications for its abundance in many tissues, bioactivity with many cell types, and excellent biocompatibility. Inspired by the structural role of lignin in plant tissue, we found that sodium …

Modeling Distributed Source Control Infrastructure At Neighborhood Scale For Mitigating Flood Risk In Amite River Watershed, Ahmad Amin Shefai

LSU Master's Theses

Flooding has always been a major threat to mankind. This is particularly true in urban areas due to urbanization-induced increasing impervious areas and subsequent increasing urban surface runoff, causing an increasing flooding risk to urban areas in spite of the fact that extensive flood control infrastructure has been constructed. The overall goal of this thesis is to present a distributed source control infrastructure-based approach for mitigating the flood risk. The new flood mitigation approach is demonstrated through the model implementation of a series of distributed source control infrastructure systems in the city of Denham Springs at a neighborhood scale. The …

Identification Of Novel Host Genes Required For Antiviral Immunity And Viral Genome Replication In C. Elegans, Fei Meng

LSU Doctoral Dissertations

RNA interference (RNAi) is a wide-spread gene silencing mechanism that control diverse biological functions and triggered by small interfering RNAs (siRNAs) processed from the viral genome or its replication intermediates. Mechanistic studies of antiviral RNAi in Caenorhabditis elegans has led to the identification of several critical factors involved in the process. As a result, whether antiviral RNAi requires additional novel genes remains to be an open question.

Viruses are intracellular parasites that rely on host products for reproduction. Disrupting their interaction with host factors can significantly compromise their replication and keep them under control. Thus, identification of host genes …

Engineering Characteristics Of Nest Building Geomaterials Used By Mud Dauber Species, Noura Saleh

LSU Master's Theses

Mud dauber wasps construct their nests using soil from adjacent areas. Mud dauber nests have been studied from an entomological perspective. However; the properties of the nest material remain unexplored from a soil engineering perspective. This study investigated the physical and mechanical properties of the nest soil for use in future bio-inspired design to improve soil behavior as a construction material. To achieve this goal, nests from three mud dauber species (black and yellow, organ pipe, and steel blue) were collected from locations near Baton Rouge, Louisiana. The shape, weight, moisture content, organic content, and specific gravity of the nests …

Regulation Of Mpcs And Kat8 During Adipogenesis And Nutritional Regulation, Jasmine Burrell

LSU Doctoral Dissertations

Obesity is a global epidemic characterized by an expansion of adipose tissue. Adipose tissue, composed of adipocytes and a stromal vascular fraction, is an endocrine organ that regulates whole body homeostasis. Obesity leads to the dysregulation of adipocytes and is often associated with increased susceptibility to metabolic diseases such as Type II diabetes mellitus (T2DM). Understanding the mechanisms by which adipocyte function and development are regulated is crucial. Mitochondrial pyruvate carriers (MPCs) are transmembrane proteins that transport pyruvate from the cytosol into the mitochondrial matrix to generate acetyl CoA. Although MPCs have been studied extensively, their roles in adipocytes are …

P-Cresol Biogenesis During Bioremediation Aimed At Stimulating Chlorinated Solvent Dehalogenation, Alexis Gathings

LSU Master's Theses

The broad goal of the research described in this thesis was to better understand the potential for biologically mediated production of p-cresol in groundwater at a Superfund site located in south Louisiana. Previous literature reports that microorganisms known to carry out the transformation from L-tyrosine to p-cresol occur in diverse environments.

In the research described in this thesis, studies were conducted utilizing groundwater from the Superfund site during the course of enhanced in situ bioremediation practices aimed at stimulating reductive dehalogenation of chlorinated solvent contamination. Microbial communities established with groundwater sampled from the Superfund site were observed to …

Genome‐Wide Association Study For Host Genetic Factors Associated With Equine Herpesvirus Type‐1 Induced Myeloencephalopathy, Wangisa M. B. Dunuwille, Navid Yousefimashouf, Udeni B. R. Balasuriya, Nicola Pusterla, Ernest Bailey

Faculty Publications

No abstract provided.

Community Context And Dispersal Stochasticity Drive Variation In Spatial Spread, Tad Dallas, Brett Melbourne, Alan Hastings

Faculty Publications

© 2020 British Ecological Society Dispersal is a key process in shaping species spatial distributions. Species interactions and variation in dispersal probabilities may jointly influence species spatial dynamics. However, many studies examine dispersal as a neutral process, independent of community context or intraspecific variation in dispersal behaviour. Here, we use controlled, replicated communities of two Tribolium species (T. castaneum and T. confusum) to examine how intraspecific variation in dispersal behaviour and community context influence dispersal dynamics in simple experimental landscapes composed of homogeneous habitat patches. We found considerable individual-level variation in dispersal probability that was unrelated to body size variation. …

Early Intracellular Trafficking And Subsequent Activity Of Programmed Cell Death In Channel Catfish Macrophages Infected With Edwardsiella Ictaluri, Lidiya P. Dubytska, Ronald L. Thune

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The development of Edwardsiella-containing-vacuoles (ECV) and the ability of Edwardsiella ictaluri to survive and replicate within macrophages suggests a unique process relative to normal phagosomal/lysosomal maturation and programed cell death. Developing ECV showed that endosomal membrane markers Rab5, EEA1, and Rab7 were all detected in both the wild type (WT) and an E. ictaluri type-3 secretion system (T3SS) mutant, 65ST. Co-localization with Lamp1, however, was significantly lower in the WT. The host cell endoplasmic reticulum marker, calnexin, co-localized to 65ST ECV significantly more than WT ECV, while Golgi vesicle marker, giantin, …

Speciation-By-Depth On Coral Reefs: Sympatric Divergence With Gene Flow Or Cryptic Transient Isolation?, Carlos Prada, Michael E. Hellberg

Faculty Publications

The distributions of many sister species in the sea overlap geographically but are partitioned along depth gradients. The genetic changes leading to depth segregation may evolve in geographic isolation as a prerequisite to coexistence or may emerge during primary divergence leading to new species. These alternatives can now be distinguished via the power endowed by the thousands of scorable loci provided by second-generation sequence data. Here, we revisit the case of two depth-segregated, genetically isolated ecotypes of the nominal Caribbean candelabrum coral Eunicea flexuosa. Previous analyses based on a handful of markers could not distinguish between models of genetic exchange …

Single-Case Experimental Designs For Behavioral Neuroscience, Paul L. Soto

Faculty Publications

Single-case experimental designs (SCEDs) are commonly used in behavior analytic research but rarely used in behavioral neuroscience research. The recent development of technologies that allow control of the timing of neurobiological events such as gene expression and neuronal firing enable the fruitful application of SCEDs for the study of brain-behavior relations. There are at least 3 benefits expected from applying SCEDs to study how neurobiological events affect behavior. First, SCEDs entail direct within- and across-subject assessments of reliability, likely increasing the probability of replication across studies and encouraging a search for the causes of replication failure when they occur. Second, …

Effects Of Cold Storage On Life-History Traits Of Aphidius Matricariae, Mehran Rezaei, Ali A. Talebi, Yaghoub Fathipour, Javad Karimzadeh, Mohammad Mehrabadi, Gadi V.P. Reddy

Faculty Publications

Aphidius matricariae Haliday (Hymenoptera: Braconidae) is a polyphagous solitary endoparasitoid, attacking more than 40 species of aphids. This parasitoid is an important commercial product of many companies that produce biological control agents. Storage at low temperature increases the shelf life of many biocontrol agents, allowing companies to provide a steady and sufficient supply of insects for biocontrol programs. In the current study, the effects of cold storage of 1-day-old host mummies with A. matricariae for various time periods (5, 10, 15, 20, and 30 days) at 5 °C on the parasitoid’s key life-history traits were investigated. Parameters assessed after storage …

Novel Targets In Glucose Homeostasis And Obesity—Lesson From Rare Mutations, Bing Feng, Pingwen Xu, Yanlin He

Division of Basic Science Faculty Publications

Purpose of Review: Obesity and diabetes have already become the second largest risk factor for cardiovascular disease. During the last decade, remarkable advances have been made in understanding the human genome’s contribution to glucose homeostasis disorders and obesity. A few studies on rare mutations of candidate genes provide potential genetic targets for the treatment of diabetes and obesity. In this review, we discussed the detailed findings of these studies and the possible causalities between specific genetic variations and dysfunctions in energy or glucose homeostasis. We are optimistic that novel therapeutic strategies targeting these specific mutants for treating and preventing diabetes …

Indirect, Non-Thermal Atmospheric Plasma Promotes Bacterial Killing In Vitro And Wound Disinfection In Vivo Using Monogenic And Polygenic Models Of Type 2 Diabetes (Without Adverse Metabolic Complications), Chris R. Cooley, John Mark Mclain, Samuel D. Dupuy, Adrianna E. Eder, Molly Wintenberg, Kimberly Kelly-Wintenberg, Alan Wintenberg, J. Jason Collier, Susan J. Burke, Michael D. Karlstad

Division of Basic Science Faculty Publications

ABSTRACTA novel atmospheric plasma device that uses indirect, non-thermal plasma generated from room air is being studied for its effects on wound disinfection in animal wounds of monogenic and polygenic murine models of type 2 diabetes. As a proof-of-concept report, the goal of this study was to demonstrate the efficacy and safety of the indirect non-thermal plasma (INTP) device in disinfecting polycarbonate filters established with Pseudomonas aeruginosa (PAO1) biofilms as well as wound disinfection in diabetic murine wounds. Dorsal excisional wounds in BALB/c, polygenic TALLYHO, and monogenic db/db mice established with PAO1 infection all demonstrated a 3-log colony-forming unit (CFU) …

Multi-Trait Genomic Prediction Of Yield-Related Traits In Us Soft Wheat Under Variable Water Regimes, Jia Guo, Jahangir Khan, Sumit Pradhan, Dipendra Shahi, Naeem Khan, Muhsin Avci, Jordan Mcbreen, Stephen Harrison, Gina Brown-Guedira, J. Paul Murphy, Jerry Johnson, Mohamed Mergoum, Richanrd Esten Mason, Amir M.H. Ibrahim, Russel Sutton, Carl Griffey, M. A. Babar

Faculty Publications

The performance of genomic prediction (GP) on genetically correlated traits can be improved through an interdependence multi-trait model under a multi-environment context. In this study, a panel of 237 soft facultative wheat (Triticum aestivum L.) lines was evaluated to compare single-and multi-trait models for predicting grain yield (GY), harvest index (HI), spike fertility (SF), and thousand grain weight (TGW). The panel was phenotyped in two locations and two years in Florida under drought and moderately drought stress conditions, while the genotyping was performed using 27,957 genotyping-by-sequencing (GBS) single nucleotide polymorphism (SNP) makers. Five predictive models including Multi-environment Genomic Best Linear …

Ribosomal Rna Synthesis After Induced Nucleolar Stress In Drosophila Melanogaster, Kyle Maher

Honors Capstones

No abstract provided.

Increasing The Bioavailability Of Poorly Soluble Anti-Cancer Prodrugs Through Encapsulation In Platinum Based Nanocages, Salim Cesar Lutfallah

Honors Capstones

No abstract provided.

Characterizing Drosophila Melanogaster Sleep Models Using An Optogenetic Engineered Caspase And Channelrhodopsins, Corey Cooper

Honors Capstones

No abstract provided.

Correlation Analysis Of Precipitation And River Flow With The Injection And Discharge Of The Three Gorges Dam And Reservoir, Lirong Yin

LSU Master's Theses

The Yangtze River has been the primary support of the resources and transportation of China. Its basin covers an area of 1.8 million square kilometers. The Three Gorges Dam and Reservoir on the Yangtze River is one of the world's largest dams. After the dam construction in 1997, the reservoir started injecting the reservoir to a size of over 600 km². The influence caused by the dam and reservoir on the river system has been overwhelming and destructive. The possible influence of this vast water body and the operation to maintain this waterbody's size and water level on …

Modulating Mechanical Properties Of Polymer Composites Via Colloidal Particle Reinforcement, Yusheng Guo

LSU Master's Theses

Additive manufacturing allows the rapid process of complex objects with excellent design flexibility. However, the products often exhibit poor mechanical properties when pure polymer is applied as printable material. In this work, we demonstrate that printability of polymer can be dramatically improved when particle filler is added to form reinforced polymer composites. Furthermore, the interaction between filler and polymer matrix leads to the enhancement in mechanical properties of the printed product. The material reinforcement induced by addition of fillers enables the wide application of polymer composites to print structures with unique features. In the printing of silica-reinforced pNIPAM composite, we …

Verbal Response Inhibition And Stuttering In Adults, Shanley Belle Treleaven

LSU Doctoral Dissertations

Many adults who stutter (AWS) attempt to modify or suppress their stuttered speech daily. The ability to effectively suppress motoric behavior after initiation relies on executive functions such as inhibition – specifically verbal inhibition – a challenging task regardless of clinical status. Minimal published data are available about verbal inhibition in non-stuttering adults, and no data are available for AWS. Researchers have reported slower inhibition for AWS during manual tasks, but inconsistent relationships have been found between manual and verbal inhibition. It is often presumed that inhibition differences in AWS, if detected, would be associated with greater difficulties suppressing the …

A Vaccine Targeting The Rbd Of The S Protein Of Sars-Cov-2 Induces Protective Immunity, Jingyun Yang, Wei Wang, Zimin Chen, Shuaiyao Lu, Fanli Yang, Zhenfei Bi, Linlin Bao, Fei Mo, Xue Li, Yong Huang, Weiqi Hong, Yun Yang, Yuan Zhao, Fei Ye, Sheng Lin, Wei Deng, Hua Chen, Hong Lei, Ziqi Zhang, Min Luo, Hong Gao, Yue Zheng, Yanqiu Gong, Xiaohua Jiang, Yanfeng Xu, Qi Lv, Dan Li, Manni Wang, Fengdi Li, Shunyi Wang, Guanpeng Wang, Pin Yu, Yajin Qu, Li Yang

Division of Basic Science Faculty Publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a respiratory disease called coronavirus disease 2019 (COVID-19), the spread of which has led to a pandemic. An effective preventive vaccine against this virus is urgently needed. As an essential step during infection, SARS-CoV-2 uses the receptor-binding domain (RBD) of the spike protein to engage with the receptor angiotensin-converting enzyme 2 (ACE2) on host cells1,2. Here we show that a recombinant vaccine that comprises residues 319–545 of the RBD of the spike protein induces a potent functional antibody response in immunized mice, rabbits and non-human primates (Macaca mulatta) as early as 7 …

Novel Information About The Kinetic Effects Of Equine Shoe Modifications And Kinematic Effects Of Human Digital Devices For Improved Performance In Both Species, Pengju Wang

LSU Master's Theses

Equine shoes are frequently modified to enhance traction for horses that travel on paved surfaces for work, pleasure, or entertainment. Little is known about other common shoe modifications used to enhance traction like calks, tungsten carbide granules, or plastic composition. This information is vital to shoe design to protect the safety and welfare of all service, working, and leisure horses. The objective of the first part of this thesis was to quantify the effect of shoes with and without traction adaptions on kinetic measures in non-lame, light breed horses at a trot. Kinetic data was collected with a force platform …

Distribution Of Aromatase In The Brain Of The African Cichlid Fish Astatotilapia Burtoni: Aromatase Expression, But Not Estrogen Receptors, Varies With Female Reproductive-State, Karen P. Maruska, Julie M. Butler, Chase Anselmo, Ganga Tandukar

Faculty Publications

© 2020 Wiley Periodicals, Inc. Estrogen synthesis and signaling in the brains of vertebrates has pleotropic effects ranging from neurogenesis to modulation of behaviors. The majority of studies on brain-derived estrogens focus on males, but estrogenic signaling in females likely plays important roles in regulation of reproductive cycling and social behaviors. We used females of the mouth brooding African cichlid fish, Astatotilapia burtoni, to test for reproductive state-dependent changes in estrogenic signaling capacity within microdissected brain nuclei that are important for social behaviors. Expression levels of the rate-limiting enzyme aromatase, but not estrogen receptors, measured by qPCR changes across the …

Neural Activation Patterns Associated With Maternal Mouthbrooding And Energetic State In An African Cichlid Fish, Karen P. Maruska, Julie M. Butler, Karen E. Field, Christopher Forester, Ashley Augustus

Faculty Publications

© 2020 IBRO Parental care is widespread in the animal kingdom, but for many species, provisioning energetic resources must be balanced with trade-offs between self-promoting and offspring-promoting behaviors. However, little is known about the neural mechanisms underlying these motivational decisions. Mouthbrooding is an extreme form of parental care most common in fishes that provides an ideal opportunity to examine which brain regions are involved in parenting and energetics. The African cichlid fish Astatotilapia burtoni is a maternal mouthbrooder in which females hold developing young inside their mouths for 2 weeks. This brood care makes feeding impossible, so females undergo obligatory …

The Life History And Population Dynamics Of Southern Flounder, Kenneth A. Erickson

LSU Master's Theses

Southern Flounder, Paralichthys lethostigma, are a coastal, estuarine-dependent flatfish species that inhabits the Southeastern US Atlantic Ocean and Gulf of Mexico. Throughout their range, Southern Flounder are exploited by both commercial and recreational fisheries. Recently, numerous sources have expressed concerns about Southern Flounder populations, with stock assessments indicating declines in spawning stock biomass and recruitment. To estimate life history traits needed for informed management, such as age, growth, and maturity, I collected 327 Southern Flounder from Louisiana and obtained 14,184 historical records from the Louisiana Department of Wildlife and Fisheries. Using a von Bertalanffy growth equation in a Bayesian …

Higher Csf Strem2 Attenuates Apoe4-Related Risk For Cognitive Decline And Neurodegeneration, Nicolai Franzmeier, M. Suárez-Calvet, Lukas Frontzkowski, Annah Moore, Timothy J. Hohman, Estrella Morenas-Rodriguez, Brigitte Nuscher, Leslie Shaw, John Q. Trojanowski, Martin Dichgans, Gernot Kleinberger, Christian Haass, Michael Ewers, Michael Weiner, Paul Aisen, Gerald Novak, Robert C. Green, Tom Montine, Ronald Petersen, Anthony Gamst, Ronald G. Thomas, Michael Donohue, Sarah Walter, Devon Gessert, Tamie Sather, Laurel Beckett, Danielle Harvey, John Kornak, Clifford R. Jack, Anders Dale, Matthew Bernstein, Joel Felmlee, Nick Fox, Paul Thompson

Clinical Research Faculty Publications

Background: The Apolipoprotein E ϵ4 allele (i.e. ApoE4) is the strongest genetic risk factor for sporadic Alzheimer's disease (AD). TREM2 (i.e. Triggering receptor expressed on myeloid cells 2) is a microglial transmembrane protein brain that plays a central role in microglia activation in response to AD brain pathologies. Whether higher TREM2-related microglia activity modulates the risk to develop clinical AD is an open question. Thus, the aim of the current study was to assess whether higher sTREM2 attenuates the effects of ApoE4-effects on future cognitive decline and neurodegeneration. Methods: We included 708 subjects ranging from cognitively normal (CN, n = …