Digital Commons Network^™

Population Structure, Gene Flow, And Sex-Biased Dispersal In The Reticulated Flatwoods Salamander (Ambystoma Bishopi): Implications For Translocations, Steven T. Williams, Jean P. Elbers, Sabrina S. Taylor

Faculty Publications

Understanding patterns of gene flow and population structure is vital for managing threatened and endangered species. The reticulated flatwoods salamander (Ambystoma bishopi) is an endangered species with a fragmented range; therefore, assessing connectivity and genetic population structure can inform future conservation. Samples collected from breeding sites (n = 5) were used to calculate structure and gene flow using three marker types: single nucleotide polymorphisms isolated from potential immune genes (SNPs), nuclear data from the major histocompatibility complex (MHC), and the mitochondrial control region. At a broad geographical scale, nuclear data (SNP and MHC) supported gene flow and little structure (FST …

Moringa Oleifera Seed Extract Concomitantly Supplemented With Chemotherapy Worsens Tumor Progression In Mice With Triple Negative Breast Cancer And Obesity, Elizabeth R.M. Zunica, Shengping Yang, Ann Coulter, Christy White, John P. Kirwan, Linda A. Gilmore

Division of Basic Science Faculty Publications

Triple negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype with limited treatment options. Obesity and insulin resistance are associated with a worse prognosis in those with TNBC. Moringa oleifera (moringa) is a tropical edible plant used for both food and medicinal purposes and found to have anti-obesity and anti-cancer effects in vitro and in preclinical models. The anti-cancer effects of moringa seed extract alone and in combination with chemotherapy were evaluated in immunocompromised female mice with diet-induced obesity bearing MDA-MB-231-derived xenograft tumors. Moringa supplementation protected against high-fat diet-and chemotherapy-induced increases in fasting glucose and improved …

Eating Behaviors And Dietary Patterns Of Women During Pregnancy: Optimizing The Universal ‘Teachable Moment’, Maryam Kebbe, Emily W. Flanagan, Joshua R. Sparks, Leanne M. Redman

Clinical Research Faculty Publications

Understanding women’s perceptions of eating behaviors and dietary patterns can inform the ‘teachable moment’ model of pregnancy. Our objectives were to describe eating behaviors and dietary patterns in pregnancy. This was a cross‐sectional, national electronic survey. Women were ≥18 years of age, living in the United States, currently pregnant or less than two years postpartum, and had internet access. Age, education, race, and marriage were included as covariates in ordinal and binary logistic regressions (significance p < 0.05). Women (n = 587 eligible) made positive or negative changes to their diets, while others maintained pre‐existing eating behaviors. The majority of women did not try (84.9 to 95.1% across diets) and were unwilling to try (66.6 to 81%) specific dietary patterns during pregnancy. Concerns included not eating a balanced diet (60.1 to 65.9%), difficulty in implementation without family (63.2 to 64.8%), and expense (58.7 to 60.1%). Helpful strategies included being provided all meals and snacks (88.1 to 90.6%) and periodic consultations with a dietitian or nutritionist (85 to 86.7%). Responses differed across subgroups of parity, body mass index, and trimester, notably in women with obesity who reported healthier changes to their diet (p < 0.05). Our study underscores the importance of tailoring care early to individual needs, characteristics, and circumstances.

Cage's Imitation Game: Cheap Imitation And Song Books Through The Sketches, Jeffrey Perry

Faculty Publications

The theme of John Cage’s Song Books (1970), according to Cage, is contained in the statement “We connect Satie with Thoreau” (Cage 1970a, 1). Previous studies of Cage’s Song Books have not asked what I feel to be obvious questions: how, precisely, does Cage connect Satie with Thoreau? To what end? And how does Cage connect to Satie and Thoreau (and to the other sources from which he borrows)? I make use of Cage’s sketch materials to seek answers. I examine three of the Solos for Voice from Song Books that make use of the cheap-imitation procedure that Cage had …

Organic Cotton Clothing Purchase Behavior: A Comparative Study Of Consumers In The United States And Bangladesh, Md Nakib Ul Hasan, Chuanlan Liu, Bulbul Ahmed

Faculty Publications

The purpose of this research was to evaluate the differences and similarities of organic cotton clothing (OCC) purchase behaviors of the consumers who lie at the top and the bottom of the apparel supply chain. The influences of consumers’ sustainability knowledge and social norms on consumers’ attitudes and purchase intentions were examined to understand within the framework of the Theory of Reasoned Action (TRA). Sample data were collected from the United States and Bangladesh and, finally, 136 useable responses were used for the data analysis. Among the useable responses, 85 samples were from the US (containing 91.76% female participants and …

Autonomous Vehicle Communication Strategies Modeled In Virtual Reality, Nick N. Ferenchak Ph.D

Publications

We sought to better understand how autonomous vehicle (AV) communication strategies impact human road users’ perceptions and behaviors. More specifically, we explored the impact of different external human-machine interface (eHMI) designs on understanding, task load, comfort, trust, acceptance, and reaction time. To accomplish this, we created virtual reality (VR) scenarios where human participants interacted with AVs. Participants experienced biking, driving, and pedestrian simulators and were brought back after initial testing to explore acclimation and learning effects. In terms of perceptions, the presence of an eHMI was the strongest predictor of understanding, comfort, trust, and acceptance outcomes in the statistical models …

Sympathetic Innervation Of The Mouse Kidney And Liver Arising From Prevertebral Ganglia, Hayden Torres, Clara Huesing, David H. Burk, Adrien J.R. Molinas, Winfried L. Neuhuber, Hans Rudolf Berthoud, Heike Münzberg, Andrei V. Derbenev, Andrea Zsombok

Division of Basic Science Faculty Publications

The sympathetic nervous system (SNS) plays a crucial role in the regulation of renal and hepatic functions. Although sympathetic nerves to the kidney and liver have been identified in many species, specific details are lacking in the mouse. In the absence of detailed information of sympathetic prevertebral innervation of specific organs, selective manipulation of a specific function will remain challenging. Despite providing major postganglionic inputs to abdominal organs, limited data are available about the mouse celiac-superior mesenteric complex. We used tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DbH) reporter mice to visualize abdominal prevertebral ganglia. We found that both the TH …

Understanding The Etiology Of Taproot Decline Of Soybean Caused By Xylaria Necrophora, José E. Ortiz Solórzano

LSU Master's Theses

Taproot decline (TRD) of soybean is caused by Xylaria necrophora. However, the mechanisms by which it infects soybean and produces symptoms are unknown. Symptoms of TRD include foliar interveinal chlorosis and necrosis and root necrosis, yet X. necrophora appears to be restricted to the roots. While Xylaria species are predominantly wood decomposers, a few are pathogens. More importantly, they produce secondary metabolites (SMs) with multiple bioactivities, including phytotoxicity and antimicrobial activity. Preliminary experiments suggested that X. necrophora was producing SMs responsible for TRD symptoms. We evaluated cell-free culture filtrates (CFs) for phytotoxicity on soybeans, testing for changes in chlorophyll …

In-Between The Wind, Victoria L. Vontz

LSU Master's Theses

In-between the Wind is a compilation of poems, short stories, theories, photographs, and drawings that reveal my relationship and connection with nature. Through prose, I expose and question my place in the world, how I see it and how I am connected to it, while photographic images and drawings leave space for thoughtful and reflective meditation. The work draws upon memories, discusses theories of connection, and aims to record ephemeral moments that often seem to be too easily forgotten.

The Bidirectional Effects Of Serious Conduct Problems, Anxiety, And Trauma Exposure: Implications For Our Understanding Of The Development Of Callous-Unemotional Traits, Emily Lynne Robertson

LSU Doctoral Dissertations

The association of anxiety and trauma with childhood conduct problems has long been the focus of research, and more recently this area of research has become critical to understanding the development of callous-unemotional (CU) traits. Research in samples of children and adolescents has indicated that those elevated on both CU traits and anxiety seem to show more severe externalizing behaviors and are more likely to show histories of trauma. These findings have typically been interpreted as being indicative of a unique casual pathway to CU traits in those high on anxiety. However, an alternative explanation is that the higher rates …

The Emergence Of The Legitimacy Of Religious Healing Knowledge In Taiwan, Wei-Cheng Chiu

LSU Doctoral Dissertations

This dissertation investigates how religious healing knowledge has been defined and used by scholars, physicians, and New Agers and how religious healing knowledge emerged as a legitimate area of knowledge in Taiwan. The key position of religious healing knowledge within the entanglement between religion and spirituality is also examined. After the martial law was lifted in 1987, Taiwan’s society had rapidly diversified, and its religions were at the transition point between the old and the new. Meanwhile, the new spirituality culture was introduced into Taiwan in the 1980s and got popular in the 1990s, and it inherited the trend of …

Sex-Specific Spatial Use Of The Winter Foraging Areas By Magellanic Penguins And Assessment Of Potential Conflicts With Fisheries During Winter Dispersal, Samanta Dodino, Vitor Hugo Rodrigues Paiva, Nicolás A. Lois, Luciana Riccialdelli, Michael J. Polito, Klemens Pütz, Andrea Raya Rey

Faculty Publications

No abstract provided.

Gene (Co)Expression Networks And Alternate Transcripts Induced In Cotton In Response To Aspergillus Flavus Infection, Mohit Verma

LSU Doctoral Dissertations

Cotton (Gossypium hirsutum L) is an economically important crop that generates billions of dollars in annual revenue worldwide. However, contamination of cottonseed with a highly carcinogenic aflatoxin produced by Aspergillus flavus limits the use of cottonseed in the oil and animal feed industries. Much success has been achieved by the application of atoxigenic strains of A. flavus for controlling aflatoxin contamination in cotton, peanut, and maize. Understanding the gene (co)expression network in response to A. flavus infection with both toxigenic and atoxigenic strains of A. flavus pericarp and seed of cotton through genome-wide transcriptome profiling will not only unravel …

Assessment Of Risks To Listed Species From The Use Of Atrazine In The Usa: A Perspective, Philip N. Smith, Kevin L. Armbrust, Richard A. Brain, Wenlin Chen, Nika Galic, Lula Ghebremichael, Jeffrey M. Giddings, Mark L. Hanson, Jonathan Maul, Glen Van Der Kraak, Keith R. Solomon

Faculty Publications

No abstract provided.

Learning To Interpret Fluid Type Phenomena Via Images, Simron Thapa

LSU Doctoral Dissertations

Learning to interpret fluid-type phenomena via images is a long-standing challenging problem in computer vision. The problem becomes even more challenging when the fluid medium is highly dynamic and refractive due to its transparent nature. Here, we consider imaging through such refractive fluid media like water and air. For water, we design novel supervised learning-based algorithms to recover its 3D surface as well as the highly distorted underground patterns. For air, we design a state-of-the-art unsupervised learning algorithm to predict the distortion-free image given a short sequence of turbulent images. Specifically, we design a deep neural network that estimates the …

Devices And Methods For Deep Uv Laser Ablation, Kermit K. Murray, Fabrizio Donnarumma, Oluwaremilekun Lawai

College of Science Patents

Laser ablation devices and methods including laser ablation are provided. The ablation devices can include a deep UV laser. Dual-laser ablation devices are also provided. Biomolecules can be ablated using a combination of deep UV laser and nanoelectrospray, resulting in protonated sample molecules.

Characterization Of Application-Driven Sol-Gel Coated Meshes And Composites, Armaghan Naderi

LSU Doctoral Dissertations

Surface engineering is a multi-disciplinary research area that encompasses disciplines such as materials science, chemistry, mechanical engineering, and electrical engineering. Coating is a surface modification technique in surface engineering, applied on the surface of a substrate (i.e., bulk material), to cover/protect it from environmental degradation or to modify/improve surface properties for specific applications. This PhD research presents an in-depth investigation of sol-gel ceramic coatings to modify surface characteristics of bulk materials for two specific applications: 1) antistatic/antidust applications, and 2) biomaterial implant applications.

First, zirconia antistatic thin films on glass substrates, and their application to polymer-based composites, was studied. Zirconia …

Daily Energy Expenditure Through The Human Life Course, Herman Pontzer, Yosuke Yamada, Hiroyuki Sagayama, Philip N. Ainslie, Lene F. Andersen, Liam J. Anderson, Lenore Arab, Issaad Baddou, Kweku Bedu-Addo, Ellen E. Blaak, Stephane Blanc, Alberto G. Bonomi, Carlijn V.C. Bouten, Pascal Bovet, Maciej S. Buchowski, Nancy F. Butte, Stefan G. Camps, Graeme L. Close, Jamie A. Cooper, Richard Cooper, Sai Krupa Das, Lara R. Dugas, Ulf Ekelund, Sonja Entringer, Terrence Forrester, Barry W. Fudge, Annelies H. Goris, Michael Gurven, Catherine Hambly, Asmaa El Hamdouchi, Marjije B. Hoos, Sumei Hu, Noorjehan Joonas

Faculty Publications

Total daily energy expenditure (“total expenditure”) reflects daily energy needs and is a critical variable in human health and physiology, but its trajectory over the life course is poorly studied. We analyzed a large, diverse database of total expenditure measured by the doubly labeled water method for males and females aged 8 days to 95 years. Total expenditure increased with fat-free mass in a power-law manner, with four distinct life stages. Fat-free mass-adjusted expenditure accelerates rapidly in neonates to ~50% above adult values at ~1 year; declines slowly to adult levels by ~20 years; remains stable in adulthood (20 to …

Analytical Determination Of Mitochondrial Function Of Excised Solid Tumor Homogenates, Elizabeth R.M. Zunica, Christopher L. Axelrod, L. Anne Gilmore, John P. Kirwan

Division of Basic Science Faculty Publications

Mitochondria are essential to the onset and progression of cancer through energy production, reactive oxygen species regulation, and macromolecule synthesis. Genetic and functional adaptations of mitochondria to the tumor environment drive proliferative and metastatic potential. The advent of DNA and RNA sequencing removed critical barriers to the evaluation of genetic mediators of tumorigenesis. However, to date, methodological approaches to evaluate tumor mitochondrial function remain elusive and require technical proficiency limiting the feasibility, ultimately diminishing diagnostic and prognostic value in both experimental and clinical settings. Here, we outline a simple and rapid method to quantify rates of oxidative phosphorylation (OXPHOS) and …

Effect Of Disclosed Information On Product Liking, Emotional Profile, And Purchase Intent: A Case Of Chocolate Brownies Containing Edible-Cricket Protein, Cristhiam E. Gurdian, Damir D. Torrico, Bin Li, Georgianna Tuuri, Witoon Prinyawiwatkul

Faculty Publications

Edible insects, a sustainable and nutritious alternative to conventionally derived proteins, are unfamiliar to Westerners and often associated with negative sentiments. Edible-cricket protein (ECP) added to chocolate brownies (CB) [0% ECP = CBWO (without) vs. 6% w/w ECP = CBW (with)], and disclosed information [no ECP added = (−) vs. ECP with benefits = (+), ECP− and ECP+, respectively] yielded four CB treatments (CBWO−, CBWO+, CBW−, and CBW+). Subjects (n = 112 female and n = 98 male) rated liking, selected emotions before-and after-tasting, and determined consumption (CI) and purchase intent (PI) after tasting. Likings were analyzed with mixed-effects ANOVA …

Gain-Of-Function Polymorphisms In Human Inflammasomes: Implications For Cystic Fibrosis, Duane Jeansonne, Samithamby Jeyaseelan

Faculty Publications

No abstract provided.

The Neural Underpinnings Of Processing Newly Taught Semantic Information: The Role Of Retrieval Practice, Eileen Haebig, Laurence B. Leonard, Patricia Deevy, Jennifer Schumaker, Jeffrey D. Karpicke, Christine Weber

Faculty Publications

Purpose: Recent behavioral studies have demonstrated the effectiveness of implementing retrieval practice into learning tasks for children. Such approaches have revealed that repeated spaced retrieval (RSR) is particularly effective in promoting children’s learning of word form and meaning information. This study further examines how retrieval practice enhances learning of word meaning information at the behavioral and neural levels. Method: Twenty typically developing preschool children were taught novel words using an RSR learning schedule for some words and an immediate retrieval (IR) learning schedule for other words. In addition to the label, children were taught two arbitrary semantic features for each …

The Role Of Reduced Methionine In Mediating The Metabolic Responses To Protein Restriction Using Different Sources Of Protein, Han Fang, Kirsten P. Stone, Sujoy Ghosh, Laura A. Forney, Thomas W. Gettys

Division of Basic Science Faculty Publications

Dietary protein restriction and dietary methionine restriction (MR) produce a comparable series of behavioral, physiological, biochemical, and transcriptional responses. Both dietary regimens produce a similar reduction in intake of sulfur amino acids (e.g., methionine and cystine), and both diets increase expression and release of hepatic FGF21. Given that FGF21 is an essential mediator of the metabolic phenotype produced by both diets, an important unresolved question is whether dietary protein restriction represents de facto methionine restriction. Using diets formulated from either casein or soy protein with matched reductions in sulfur amino acids, we compared the ability of the respective diets to …

Range-Wide Population Genetic Analysis Of Seaside Sparrows (Ammospiza Maritima) Supports At Least Five Distinct Population Segments That Do Not Align With Current Subspecies Descriptions, Kathryn E.C. Davis, Amie E. Settlecowski, Mackenzie R. Roeder, Carolyn Enloe, Thomas Virzi, Margaret E. Hunter, Stefan Woltmann, Sabrina S. Taylor

Faculty Publications

As an obligate salt marsh species, Seaside Sparrows (Ammospiza maritima) are vulnerable to numerous threats including climate change, coastal erosion, sea-level rise, and both natural and anthropogenic disasters. Of the 9 recognized subspecies, 2 are extinct and 1 is endangered. Previous genetic analyses of mitochondrial DNA (mtDNA) and microsatellite loci showed that current taxonomy does not accurately reflect underlying genetic diversity, with possible consequences for the distribution of conservation resources. To further inform Seaside Sparrow management, we comprehensively describe genetic structure among 24 range-wide sampling locations that include all extant subspecies. We inferred population structure from several thousand single-nucleotide polymorphisms …

Comparing Ultraconserved Elements And Exons For Phylogenomic Analyses Of Middle American Cichlids: When Data Agree To Disagree, Fernando Alda, William B. Ludt, Diego J. Elías, Caleb D. Mcmahan, Prosanta Chakrabarty

Faculty Publications

Choosing among types of genomic markers to be used in a phylogenomic study can have a major influence on the cost, design, and results of a study. Yet few attempts have been made to compare categories of next-generation sequence markers limiting our ability to compare the suitability of these different genomic fragment types. Here, we explore properties of different genomic markers to find if they vary in the accuracy of component phylogenetic trees and to clarify the causes of conflict obtained from different data sets or inference methods. As a test case, we explore the causes of discordance between phylogenetic …

Evaluation Of Cell Concentration And Viability By Impedance Spectroscopy On Microfluidic Devices, Jason Eades

LSU Master's Theses

This document describes two distinct platforms that implement electrochemical impedance spectroscopy (EIS) within microfluidic devices for rapid, label-free cell analysis. Each study provides proof-of-concept evaluations of these devices for cell counting and viability analysis applications to mitigate some of the challenges associated with conventional methods. Chapter one includes background information on each version of EIS selected and motivations for the studies conducted. Chapter two describes the design and fabrication of a modular, reusable microfluidic device. Additionally, the methodology for and results from the application of this platform for the measurement of zebrafish sperm cell concentrations are presented. Chapter three describes …

Informing Viral Surveillance Of Oropouche Virus: An Emerging Arbovirus, Christine E. Walsh

LSU Master's Theses

Arboviruses are viruses transmitted by hematophagous arthropod vectors. Many of these viruses pose a threat to human health. While some infections may be asymptomatic, others may produce febrile illness, or manifest in life threatening conditions. Symptomatic diagnosis of patients with generalized febrile illness can be confounded due to the various diseases that present clinically in the same manner, and a lack of available diagnostic tests. Surveillance efforts can be used to create awareness about the prevalence of neglected tropical arthropod-borne pathogens, such as Oropouche virus (OROV), and inform preparedness measures for disease outbreaks (SURVEILLANCE SENTENCE). OROV is an arbovirus and …

Sulfur And Sage, John Swincinski

LSU Master's Theses

Sulfur and Sage is a body of abstract paintings created out of memories of time spent immersed in the wilderness of the Rocky Mountains and the Yellowstone Caldera during the summer of 2020. The paintings reflect specific places, experiences, and insights, which occurred as I sought solitude by returning to the one place where I feel most at peace with the universe – the woods.

Each painting is augmented by a written vignette, describing the occurrence it is based on. Should the viewer choose to include these short stories as they encounter the artwork, they provide the jumping off point …

Investigating Local Adaptation To Hypoxia Stress In The Eastern Oyster Through Comparative Transcriptomics, Heather Nichole Smith

LSU Master's Theses

Climate change represents one of the most important challenges to biodiversity, therefore it is important to understand the mechanisms that allow species to respond to rapid environmental change. Here, we compared two populations of eastern oysters, Crassostrea virginica, from the Gulf of Mexico to study the mechanisms underlying hypoxia tolerance. Using a common garden experiment and comparative transcriptomics, we identified sets of genes involved in the hypoxia response and found differences in both the timing and baseline expression of hypoxia-responsive genes between tolerant and sensitive populations, consistent with a scenario of local adaptation. These genes include the signaling transcription factor …

Evaluation Of Cool-Season Annuals Effect On Soil Health In Warm-Season Perennial Pastures In Southestern Us., Paola C. Muela Negrete

LSU Master's Theses

Perennial grass crops represent approximately 8 million hectares of the land area of the humid lower southeastern United States. These forage crops receive high rates of fertilizer, especially nitrogen (N), and near monoculture remains have often been treated with repeated applications of herbicides. Pasture management is crucial to improve soil properties in pasturelands. Common pasture management practices include introducing cool-season multispecies in warm-season pasture systems and forage harvest frequency of pasture systems. It is known that cool-season multispecies in warm-season pasture systems ensure cattle feeding during winter season and have beneficial effects on soil microbial biomass, soil organic matter (SOM), …