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3-Dimensional Structural Determination Of Epidermal Growth Factor-Like Repeat 27 From Mouse Notch1 Using Nuclear Magnetic Resonance Spectroscopy, Justin Grennell

LSU Doctoral Dissertations

NOTCH1 has been associated with a variety of human ailments, such as cancer and heart disease. NOTCH1 is a transmembrane protein that contains 36 epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding proteins on neighboring cells. Each repeat contains about 40 amino acids and has to be folded in a specific manner to function properly. The amino acid sequence, carbohydrate pattern, and calcium-binding sites of EGF-like repeats vary, but the pattern along the 36-repeat chain is conserved. The Abruptex region (EGF24-29) of NOTCH1 is a highly glycosylated (having undergone sugar addition) and flexible region, which gained its …

The Origin And The Evolution Of The Double Bass, Patrick James Lavergne

LSU Doctoral Dissertations

This dissertation will trace the double bass from the 16^th century to the 21^st century. The double bass is the largest and lowest pitched member of the string family. The members of the string family are the violin, viola, cello, and the double bass. The pitch of the double bass sounds one octave lower than the written pitch. The double bass is sometimes called the contrabass, string bass, upright bass, or bass fiddle. It is used in different genres of music such as classical, jazz, blues, bluegrass, country & western, and even rock.

Investigation Of Bovine Herpesvirus-1 Determinants Of Neuronal Infectivity In Novel Bovine Neuronal Cell Systems For Development Of A Safer Viral Vaccine Vector, Jared Rudd

LSU Doctoral Dissertations

Bovine herpesvirus-1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis and a component of the bovine respiratory disease complex. BoHV-1 is a significant burden on the cattle industry in the United States causing severe respiratory disease, abortions, loss of calves (“shipping fever”). BoHV-1 belongs to the alphaherpesvirus subfamily of Herpesviridae. Alphaherpesviruses enter axons of sensory nerves and establish life-long latency in trigeminal and dorsal root ganglionic neurons after primary infection of the oronasal or genital epithelium respectively. Modified live attenuated vaccines for BoHV-1 are commercially available but are limited in their protection while retaining the ability to establish latency …

Regional Expansion And Evaluation Of Potential Chemical Control For Invasive Apple Snails (Pomacea Maculata) In Southwest Louisiana, Julian M. Lucero

LSU Master's Theses

The integration of monitoring and chemical control is an efficient strategy for managing invasive apple snails, Pomacea maculata, in the rice (Oryza sativa L.) and crawfish systems of southwest Louisiana. However, their current distribution, expansion rates, and susceptibility to chemical control methods in this area are not well known. This study evaluated the expansion of P. maculata in southwest Louisiana and assessed potential chemical control for P. maculata among toxicity assays using various application rates. The effects of potential chemical control were also assessed on a non-target species, the red swamp crawfish (Procambarus clarkii). P. maculata …

Brood Abundance And Invertebrate Availability In Crop-Dominated Landscapes In The Prairie Pothole Region, Catrina V. Terry

LSU Master's Theses

The Prairie Pothole Region (PPR) is the most important region for ducks in North America, producing over half the ducks on the continent, and it is a priority landscape for wetland and grassland conservation. Agricultural expansion has changed the PPR, and the majority of grasslands and potholes have been converted into row-crops. The loss of nesting habitat has directly caused nest success to decline. Most of the remaining wetlands are surrounded by row-crops, and are considered lower quality because they receive runoff of sediment and chemicals, which may decrease primary forage for young ducklings as well as inhibit wetland vegetation. …

Fish Gelatin Coating As An Antimicrobial Agent On Wild-Caught Louisiana Shrimp, Hunter S. Songy

LSU Master's Theses

Seafood has a short shelf-life because of its unstable nature. Shrimp has a shelf-life of only eight days. Edible coatings, like those made from gelatin, have been used to apply antimicrobial agents to food product surfaces. Organic acids are naturally occurring, low-cost options that have been proven to effectively reduce bacterial loads on food products and extend their shelf-life. Gelatin can be extracted from catfish skin, helping to reduce product waste. The purpose of this study was to determine the potential effectiveness of fish skin gelatin as an antimicrobial coating alone and in combination with lactic acid, and then compare …

Role Of Aim2 Inflammasome In Pulmonary Host Defense Against Staphylococcus Aureus-Induced Pneumonia, Bibek Dhakal

LSU Master's Theses

Bacterial pneumonia is the leading cause of death in children under the age of five, claiming the lives of two million children each year. Staphylococcus aureus is the extracellular Gram-positive bacteria that causes necrotizing pneumonia. Moreover, the spread of antibiotic-resistant and hypervirulent strains has made treatment more challenging. Absent in melanoma 2 (AIM2) is a dsDNA-sensing cytosolic innate immune receptor that activates inflammasomes and is crucial for host defense against intracellular bacteria and DNA viruses. However, its role in extracellular bacterial pathogen-induced pneumonia is unclear. Therefore, understanding how AIM2 initiates host responses during Staphylococcus aureus is crucial for the development …

Willingness Of Non-Industrial Private Forest Landowners To Obtain A Private Versus Public Consultation, Erika Largacha

LSU Master's Theses

Promoting conservation practices among family forests landowners play a critical role to enhance forest health and ecosystem services. Several certification programs recognize such efforts, but participation among landowners varies based on their characteristics, program requirements, and incentives. One crucial step of certification is to obtain a forest management plan, but acquisition among landowners remains extremely low. Several studies suggest connecting landowners with foresters as the key element towards the adoption of forest management plans. Because foresters are typically employed by public forestry agencies or as private self-employed consultants, landowners' willingness to work with either may differ. As far as we …

Evaluation Of The Effectiveness Of Aeration During Washing To Dislodge Microorganisms From Cucumbers And Bell Peppers, Julysa Benitez

LSU Master's Theses

Microorganisms may attach firmly to produce matrices and could affect the efficacy of disinfectants. The purpose of this study is to evaluate the effectiveness of aeration to reduce microbial risk during washing and sanitizing of fresh produce. Bell peppers and cucumbers were spot inoculated with Listeria innocua, E. coli O157:H7, and Salmonella; after drying in the biosafety cabinet for 1h, these were washed with water or sanitized with 100 ppm of chlorine for 3 min with or without aeration. Bell pepper and cucumber samples were placed in sterile bags with PBS after treatment. Bell peppers were processed in the stomacher …

Corn Nitrogen Management Under Different Tillage Systems, Akshara Athelly

LSU Master's Theses

Adoption of no-tillage (NT) and cover crops (CC) significantly effect soil physicochemical properties and nutrient cycling that necessitates modified nutrient management to maximize crop yields. Two field experiments were conducted to evaluate N-sources Urea Ammonium Nitrate (UAN), urea, and urea+ N-stabilizer (urea+ stab) and N-split applications 100%N at V2 (S1), 25%N at V2+75%N at V6 (S2), 25%N at V2+ 50%N at V6+ 25%N at VT (S3) in a sandy loam soil and three CC wheat (Triticum aestivum), hairy vetch (Vicia villosa) , and radish (Raphanus sativus) and their combinations along with three N-rates ( …

What The Shell? The Zooarchaeology Of Cerro San Isidro, Peru, Monica Fenton

LSU Master's Theses

Zooarchaeologists have documented the importance of marine resources in the ancient Andes, and the first field season at Cerro San Isidro (Ancash, Peru) proves no different. The multi- component hilltop archaeological complex lies in the agriculturally rich Moro Pocket of the middle Nepeña Valley, at least an eight-hour walk from the ocean on the north-central coast. Between June and August 2019, members of the Proyecto de Investigación Arqueológica Cerro San Isidro carried out the first scientific excavations at this important ancient human settlement. Block excavations focused on a hypothesized elite compound, documenting intermittent occupations from the Late Formative (c. 600 …

Consumers' Perception Of Food Containing Insect Protein Affected By Serving Condition And Questionnaire Protocol, Yupeng Gao

LSU Master's Theses

Edible insects are a new sustainable protein source compared to meat. Western consumers show aversion to the prospect of insects as food due to unfamiliarity, food neophobia, and negative emotions. This dissertation research explored consumers’ emotional perception of insect-based food products presented under different testing conditions and examined the influence of other people’s emotional expressions towards consumers’ purchase decisions. To identify and rate emotion intensities, tests were conducted using three presenting formats including text, image, and an actual product; all of which were applied for both cookies containing insects and without insects. In addition to these presenting formats, news of …

High Precision Tissue Sampling For Mass Spectrometry, Chao Dong

LSU Doctoral Dissertations

A high precision infrared laser ablation sampling system was developed for localized tissue proteomics. A Schwarzschild reflective objective with 10 cm working distance and 0.3 numerical aperture was used to focus a mid-IR laser at 3 µm wavelength to a beam diameter of approximately 6 µm. A laser ablation spot size of 10 µm was achieved on thin ink films and tissue sections. The sampling system was used to ablate and capture rat brain tissue sections in transmission geometry. Captured tissue protein was extracted, digested, and analyzed with offline liquid chromatography tandem mass spectrometry (LC-MS/MS) for peptide and protein identification. …

Genome-Wide Analysis Of Peg3 Downstream Genes, Subash Ghimire

LSU Doctoral Dissertations

Paternally expressed gene 3 (Peg3) is an imprinted gene encoding a DNA-binding protein that is a predicted transcriptional repressor. The two studies presented here demonstrate the repressive functions of PEG3 in regulating different cellular processes and pathways, which were observed by analyzing the ChIP-seq results systematically using both bioinformatics tools and manual inspection of significant peaks. In the first part of the work, genome-wide features of ChIP-seq peaks were characterized and the potential de novo binding motif was reevaluated. Results suggested the transcriptional repressor role of PEG3 by binding to the conserved sequence motif of the target genes. …

The Influence Of Participation In Gambling Activities On The Personal Lives Of Gamblers As Perceived By Female Gamblers In Louisiana, Carondalette Turner

LSU Doctoral Dissertations

Historically, gambling has been a source of many ills within many cultures throughout the United States. During the last five years, there has been a precipitous surge in the accessibility of legalized gambling. Few studies have methodically surveyed the relationships between extent of gambling behavior and the consequences of gambling that may occur. The primary purpose of this study was to determine the influence of selected personal, and perceptual demographic characteristics on the extent of gambling behavior exhibited by female residents of Baton Rouge, Louisiana.

This study used a survey design with instrument distributed by hand at selected community organizations. …

Investigation Of Sand-Biochar Mixtures As A Potential Roadway Fill Material, Katia J. Lele Lagmago

LSU Master's Theses

Biochar is a sustainable and lightweight carbon-rich material with a high surface area and porosity. Previous studies reported that biochar can reduce soil erosion and cracking, retain contaminants, and enhance soil aggregation. Given these favorable properties, soil-biochar mixtures have the possibility to serve as a multifunctional lightweight fill material for roadway embankment applications. The purpose of this research is to develop sand-biochar mixtures as a sustainable and multifunctional lightweight fill material for roadway embankment applications.

This research investigated the consolidation and hydraulic properties of sand-biochar mixtures by (1) performing 1D consolidation tests, (2) performing permeability tests, and (3) assessing the …

An Erp Study Of The Effects Of Iconic And Nonsense Gestures On Memory Formation, Brianna E. Cairney

LSU Master's Theses

Co-speech gesture is an important part of human communication and aids in comprehension, learning, and memory. The addition of iconic gestures to speech has been shown in prior work to enhance memory for the speech. However, it remains unclear as to whether this benefit requires gestures to be meaningful, or, conversely, if any attentionally-engaging gesture will enhance memory. In the current study, we tested two theories to explain the mnemonic benefits of co-speech gesture: Dual Coding Theory, which attributes these benefits to multimodal encoding and enhanced imageability, and Attentional Highlighting Theory, which posits that gestures draw more attention to concurrent …

Protein Appetite At The Interface Between Nutrient Sensing And Physiological Homeostasis, Md Shahjalal Khan, Redin A. Spann, Heike Münzberg, Sangho Yu, Vance L. Albaugh, Yanlin He, Hans Rudolf Berthoud, Christopher D. Morrison

Division of Basic Science Faculty Publications

Feeding behavior is guided by multiple competing physiological needs, as animals must sense their internal nutritional state and then identify and consume foods that meet nutritional needs. Dietary protein intake is necessary to provide essential amino acids and represents a specific, distinct nutritional need. Consistent with this importance, there is a relatively strong body of literature indicating that protein intake is defended, such that animals sense the restriction of protein and adaptively alter feeding behavior to increase protein intake. Here, we argue that this matching of food consumption with physiological need requires at least two concurrent mechanisms: the first being …

A Reference Genome For The Nectar-Robbing Black-Throated Flowerpiercer (Diglossa Brunneiventris), A E. Hiller, R T. Brumfield, B C. Faircloth

Faculty Publications

Black-throated Flowerpiercers (Diglossa brunneiventris) are one species representing a phenotypically specialized group of tanagers (Thraupidae) that have hooked bills which allow them to feed by stealing nectar from the base of flowers. Members of the genus are widely distributed in montane regions from Mexico to northern Argentina, and previous studies of Diglossa have focused on their systematics, phylogenetics, and interesting natural history. Despite numerous studies of species within the genus, no genome assembly exists to represent these nectivorous tanagers. We described the assembly of a genome sequence representing a museum-vouchered, wild, female D. brunneiventris collected in Peru. By combining Pacific …

Resistance To Aflatoxin Accumulation In Maize Mediated By Host-Induced Silencing Of The Aspergillus Flavus Alkaline Protease (Alk) Gene, Olanike Omolehin, Yenjit Raruang, Dongfang Hu, Zhu Qiang Han, Qijian Wei, Kan Wang, Kanniah Rajasekaran, Jeffrey W. Cary, Zhi Yuan Chen

Faculty Publications

Aspergillus flavus is a fungal pathogen that infects maize and produces aflatoxins. Host-Induced Gene Silencing (HIGS) has been shown to reduce host infection by various fungal pathogens. Here, the A. flavus alkaline protease (alk) gene was targeted for silencing through HIGS. An RNAi vector carrying a portion of the alk gene was incorporated into the B104 maize genome. Four out of eight transformation events containing the alk gene, Alk-3, Alk-4, Alk-7 and Alk-9, were self-pollinated to T4/T6 generations. At T3, the Alk-transgenic lines showed up to 87% reduction in aflatoxin accumulation under laboratory conditions. T4 transgenic Alk-3 and Alk-7 lines, …

Effects Of Cover Crops On Beetle Assemblages In Tea Plantations, Li Lin Chen, Gabor Pozsgai, Xiang Yu Li, Lei Li, Gadi V.P. Reddy, Min Sheng You

Faculty Publications

Coleoptera are visible members of food webs in tea plantations, with high species richness and abundance. Many tea pests, as well as natural enemies, are members of this order, so a knowledge of how groundcovers affect beetles can aid pest management. We collected beetles in a replicated field experiment in the Wuyi Mountains, Fujian Province, China. Tea was intercropped with Paspalum notatum or Chamaecrista rotundifolia, or rows were cleared to bare ground, or in the control they were left unmanaged to allow weeds to grow naturally. Sampling, done by sweep netting and vegetation beating, was conducted monthly, between May 2006 …

Next-Generation Ensemble Projections Reveal Higher Climate Risks For Marine Ecosystems, Derek P. Tittensor, Camilla Novaglio, Cheryl S. Harrison, Ryan F. Heneghan, Nicolas Barrier, Daniele Bianchi, Laurent Bopp, Andrea Bryndum-Buchholz, Gregory L. Britten, Matthias Büchner, William W. L. Cheung, Villy Christensen, Marta Coll, John P. Dunne, Tyler D. Eddy, Jason D. Everett, Jose A. Fernandes-Salvador, Elizabeth A. Fulton, Eric D. Galbraith, Didier Gascuel, Jerome Guiet, Jasmin G. John, Jason S. Link, Heike K. Lotze, Olivier Maury, Kelly Ortega-Cisneros, Juliano Palacios-Abrantes, Colleen M. Petrik, Hubert Du Pontavice, Jonathan Rault, Anthony J. Richardson, Lynne Shannon, Yunne-Jai Shin

Faculty Publications

AbstractProjections of climate change impacts on marine ecosystems have revealed long-term declines in global marine animal biomass and unevenly distributed impacts on fisheries. Here we apply an enhanced suite of global marine ecosystem models from the Fisheries and Marine Ecosystem Model Intercomparison Project (Fish-MIP), forced by new-generation Earth system model outputs from Phase 6 of the Coupled Model Intercomparison Project (CMIP6), to provide insights into how projected climate change will affect future ocean ecosystems. Compared with the previous generation CMIP5-forced Fish-MIP ensemble, the new ensemble ecosystem simulations show a greater decline in mean global ocean animal biomass under both strong-mitigation …

Word-Learning Trajectories Influence Long-Term Recall In Children With Developmental Language Disorder And Typical Development, Justin B. Kueser, Laurence B. Leonard, Patricia Deevy, Eileen Haebig, Jeffrey D. Karpicke

Faculty Publications

Introduction: Children with developmental language disorder (DLD) have difficulties learning words. However, the severity of these difficulties can be reduced through word learning procedures that incorporate repeated spaced retrieval (RSR). Previous studies have shown positive outcomes with RSR but we still know very little about how learning unfolds with this procedure. In this study, we focus on the process of learning under RSR. Methods: We analyze the learning data from children with DLD and with typical development (TD) from three recent studies using RSR. We fit growth curves to the trial-by-trial learning data for immediate and spaced retrieval trials and …

Development Of An Open Hardware 3-D Printed Conveyor Device For Continuous Cryopreservation Of Non-Batched Samples, Yue Liu, Melissa Eskridge, Amy Guitreau, Jacob Beckham, Megan Chesnut, Leticia Torres, Terrence R. Tiersch, William Todd Monroe

Faculty Publications

A great challenge among communities participating in germplasm repository development is to obtain suitable cryopreservation equipment and devices. Commercial programmable freezers are costly and thus unaffordable to many users. Self-made devices have substantial variability, resulting in few opportunities for standardization across communities. The development of open hardware with the increasing accessibility of three-dimensional (3-D) printing offers rapid prototyping and easy fabrication of devices by users around the world at low cost. The present study explored the feasibility of developing operational prototypes of 3-D printed motorized cryopreservation devices for continuous freezing of non-batched samples. A controlled cooling conveyor device (CCCD) was …

Rickettsia Conorii Survival In Thp-1 Macrophages Involves Host Lipid Droplet Alterations And Active Rickettsial Protein Production, Paige E. Allen, Robert C. Noland, Juan J. Martinez

Division of Basic Science Faculty Publications

Rickettsia conorii is a Gram-negative, cytosolic intracellular bacterium that has classically been investigated in terms of endothelial cell infection. However, R. conorii and other human pathogenic Rickettsia species have evolved mechanisms to grow in various cell types, including macrophages, during mammalian infection. During infection of these phagocytes, R. conorii shifts the host cell's overall metabolism towards an anti-inflammatory M2 response, metabolically defined by an increase in host lipid metabolism and oxidative phosphorylation. Lipid metabolism has more recently been identified as a key regulator of host homeostasis through modulation of immune signalling and metabolism. Intracellular pathogens have adapted mechanisms of hijacking …

Leptin Treatment Prevents Impaired Hypoglycemic Counterregulation Induced By Exposure To Severe Caloric Restriction Or Exposure To Recurrent Hypoglycemia, Marina A. Duvall, Carolyn E. Coulter, Jasmin L. Gosey, Matthew J. Herrera, Cristal M. Hill, Rajvi R. Jariwala, Lauren E. Maisano, Laura A. Moldovan, Christopher D. Morrison, Ngozi V. Nwabueze, Hunter X. Sikaffy, David H. Mcdougal

Division of Basic Science Faculty Publications

Hypoglycemia-associated autonomic failure (HAAF) is a maladaptive failure in glucose counterregulation in persons with diabetes (PWD) that is caused by recurrent exposure to hypoglycemia. The adipokine leptin is known to regulate glucose homeostasis, and leptin levels fall following exposure to recurrent hypoglycemia. Yet, little is known regarding how reduced leptin levels influence glucose counterregulation, or if low leptin levels are involved in the development of HAAF. The purpose of this study was to determine the effect of hypoleptinemia on the neuroendocrine responses to hypoglycemia. We utilized two separate experimental paradigms known to induce a hypoleptinemic state: 60% caloric restriction (CR) …

Grow Fast, Die Young: Does Compensatory Growth Reduce Survival Of Juvenile Blacktip Sharks (Carcharhinus Limbatus) In The Western Gulf Of Mexico?, Philip Matich, Jeffrey D. Plumlee, Mark Fisher

Faculty Publications

Effective conservation and management necessitate an understanding of the ecological mechanisms that shape species life histories in order to predict how variability in natural and anthropogenic impacts will alter growth rates, recruitment, and survival. Among these mechanisms, the interaction between parturition timing and prey availability frequently influences offspring success, particularly when postnatal care is absent. Here, we assess how parturition timing and nursery conditions, including prey abundance and environmental conditions, influence the growth and potential survival of blacktip sharks (Carcharhinus limbatus) in western Gulf of Mexico (GOM) estuaries over their first year. Catch data from long-term gillnet monitoring allowed for …

Survival Of Western Gulf Coast Mottled Ducks (Anas Fulvigula) In The Path Of A Category 4 Hurricane, Kevin M. Ringelman, Elizabeth S. Bonczek, Joseph R. Marty, Ashley R. Booth, Alexandre L. Dopkin

Faculty Publications

Tropical cyclones are the most powerful storms on earth, causing catastrophic damage to human lives and infrastructure. Hurricanes also cause wildlife mortality when they make landfall, but the severity of these effects is difficult to quantify because data collection is either logistically impossible or deprioritized in the wake of human tragedy. On August 27, 2020, Hurricane Laura made landfall in southwestern Louisiana with maximum sustained winds of 241 kph (150 mph), making it one of the most powerful storms to strike the mainland United States. Hurricane Laura passed directly over the core breeding range of the western Gulf Coast population …

Morphological Consequences Of Climate Change For Resident Birds In Intact Amazonian Rainforest, Vitek Jirinec, Ryan C. Burner, Bruna R. Amaral, Richard O. Bierregaard, Gilberto Fernández-Arellano, Angélica Hernández-Palma, Erik I. Johnson, Thomas E. Lovejoy, Luke L. Powell, Cameron L. Rutt, Jared D. Wolfe, Philip C. Stouffer

Faculty Publications

Warming from climate change is expected to reduce body size of endotherms, but studies from temperate systems have produced equivocal results. Over four decades, we collected morphometric data on a nonmigratory understory bird community within Amazonian primary rainforest that is experiencing increasingly extreme climate. All 77 species showed lower mean mass since the early 1980s-nearly half with 95% confidence. A third of species concomitantly increased wing length, driving a decrease in mass:wing ratio for 69% of species. Seasonal precipitation patterns were generally better than temperature at explaining morphological variation. Short-term climatic conditions affected all metrics, but time trends in wing …

Mlr-1023 Treatment In Mice And Humans Induces A Thermogenic Program, And Menthol Potentiates The Effect, Candida J. Rebello, Ann A. Coulter, Andrew G. Reaume, Weina Cong, Luke A. Cusimano, Frank L. Greenway

Clinical Research Faculty Publications

A glucose-lowering medication that acts by a different mechanism than metformin, or other approved diabetes medications, can supplement monotherapies when patients fail to meet blood glucose goals. We examined the actions underlying the effects of an insulin sensitizer, tolimidone (MLR-1023) and investigated its effects on body weight. Diet-induced obesity (CD1/ICR) and type 2 diabetes (db/db) mouse models were used to study the effect of MLR-1023 on metabolic outcomes and to explore its synergy with menthol. We also examined the efficacy of MLR-1023 alone in a clinical trial (NCT02317796), as well as in combination with menthol in human adipocytes. MLR-1023 produced …