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High-Productivity Membrane Adsorbers: Polymer Surface-Modification Studies For Ion-Exchange And Affinity Bioseparations, Heather Chenette

All Dissertations

This Dissertation centers on the surface-modification of macroporous membranes to make them selective adsorbers for different proteins, and the analysis of the performance of these membranes relative to existing technology. Traditional chromatographic separations for the isolation and purification of proteins implement a column packed with resin beads or gel media that contain specific binding ligands on their exposed surface area. The productivity of this process is balanced by the effective use of the binding sites within the column and the speed at which the separation can take place, in addition to the need to maintain sufficient protein purity and bioactivity. …

An Epidemiology Of Recreational Sport Injury Rates At Clemson University, David Jameyson

All Theses

Injuries are one of the common risks associated with physical activity, and in certain populations, injury prevention methods are used to improve the leisure experience for participants. At the collegiate level of sports participation, NCAA sports programs are required to provide injury treatment and prevention options for their athletes. For participants competing in club and intramural sports, no universal requirements for injury prevention and treatment exist. This study assessed the risk of injury during sports participation at the club and intramural level in a college-aged population. Campus recreation incident reports and Certified Athletic Trainer evaluations were used to document the …

A Comparison Of Saponin And Tween 80 On The Solubility And The Degradation Enhancement Of Fluoranthene And The Influence Of Laccase On Fluoranthene Degradation, Stacia Ballew

All Theses

The ubiquitous contamination of polycyclic aromatic hydrocarbons (PAHs) in the environment require an economical approach as well as effective remediation techniques in the removal of these contaminants. Through the use of more eco-friendly and economically available biosurfactants, such as saponin, there is a possibility they could prove to be more useful surfactants than conventional synthetic surfactants for the solubilization of fluoranthene (FLA). Enhanced solubility would potentially facilitate the increased degradation by S. paucimobilis EPA505. The addition of laccase produced by Pleurotus ostreatus, a white rot fungus that produces enzymes known to facilitate or assist in the degradation of fluoranthene (FLA), …

Is It Culturally Appropriate? Evaluation Efforts For Measuring The Cultural Appropriateness In Nutrition Education Programs Targeting The Hispanic Population, Andrea Aguilar Corrales

All Theses

Background: In the United States, nearly 70% of the population is overweight. The U.S. National Health and Nutrition Examination Survey says Hispanics have the second prevalence of obesity, thus, increasing the risk of heart disease, cancer, stroke and diabetes in the largest minority group in the U.S. (U.S. Census, 2010). This requires effective and culturally appropriate interventions to promote healthy lifestyles. (WHO, 2003). Objective: The purpose of this study was to design and pilot-test a culturally appropriate tool to assure the nutrition information provided to the Hispanic population is appropriate. Design: The research design consisted of a triangulation of methods …

Antioxidant Effect Of Peach Skin Extracts From 13 Varieties Of South Carolina Grown Peaches, Yueyuan Zhang

All Theses

South Carolina is the second largest peach producing state in the United States. Every year, large quantities of peaches do not meet the fresh peach standard and are discarded or used for further processing. The waste stream from discarded and processed peaches includes the skin and this could be recovered as a rich source of antioxidants. The objective of this research was to determine the antioxidant (phenolic) content and antioxidant capacity of peach skin from various peach varieties grown in South Carolina. Chapter 1 is a literature review which covers topics of oxidation in foods, mechanism of lipid oxidation, antioxidants …

Cooking Healthy, Eating Smart (Ches): Evaluating The Feasibility Of Using Volunteers To Deliver Nutrition And Food Safety Education To Rural Older Adults, Morgan Getty

All Theses

Due to their limited resources, rural, older adults in the United States are at risk for poor diet-related health outcomes. Nutrition education is a key component in improving health outcomes in older adults. Cooking Healthy, Eating Smart (CHES) is a nine-lesson curriculum designed to teach rural, older adults culturally appropriate nutrition and food safety information. Funding to hire health professionals to deliver such a curriculum is limited, presenting the need to explore a less expensive mode of dissemination. In this community-based, participatory research study, a formative evaluation and feasibility study were conducted to examine the use of volunteers to deliver …

Repair Of The Proboscis Of Brush-Footed Butterflies (Lepidoptera: Nymphalidae), Suellen Pometto

All Theses

A key feature of the order Lepidoptera is the coilable proboscis, present in over 99% of lepidopteran species. The proboscis is used to obtain liquid nutrition, usually floral nectar. The proboscis is assembled from two elongate galeae immediately after emergence of the adult from the pupa. What happens if the galeae become separated? I studied the process of repair of the proboscis, behaviorally and functionally, at the organismal level. My research questions were as follows: 1) is the proboscis capable of repair, 2) is saliva necessary to proboscis repair, and 3) is the repaired proboscis able to acquire fluids? Test …

The Effect Of Gender And Status Difference On Saturated Fat Content Of Entree Items Chosen By Elementary School-Aged Students, Annie Anderson

All Theses

The purpose of this study was to examine participants in a National School Lunch Program (NSLP), and the effect of gender and/or participation in the NSLP on selection of high saturated fat content entrée items by the students. Nutrition information provided to parents often does not include a profile of the fats offered in school lunch items. This was true of this study's schools. However, new NSLP guidelines focus upon saturated and trans fat content in school lunches. The total kcal limit is less than 30% total fat, and saturated fat is less than or equal to 10% total kcal, …

Food, Nutrition, Culinary, And Packaging Science Undergraduate Research, Experiential Learning, And Product Development Focusing On Childhood Nutrition, Alexandra Weeks

All Theses

The objective of this research project was to develop a two-semester Applied Interdisciplinary Product Development (AIPD) course for sophomore students in the Food, Nutrition and Packaging Sciences (FNPS) department that would increase students’ confidence in skills pertaining to product development of food products and childhood nutrition, increase their sense of connection with the department, and would better prepare them to enter industry than students that did not participate in the course. A Subject Knowledge Assessment (SKA) was used to evaluate the mean difference value (MDV) of food science, nutrition, packaging science, and general product development knowledge gained through the AIPD …

Automatically Measuring Individual Consumption Events During Natural Eating Using A Table Embedded Scale, Ryan Mattfeld

All Theses

This thesis is motivated to improve the tools available for tracking energy intake. The goal of this work is to develop a table-embedded scale capable of measuring the weight of individual consumption events during unrestricted eating. The method was tested on a data set gathered from 276 subjects eating 518 courses consisting of 22,383 marked individual consumption events in a cafeteria environment. Approximately 30% of the consumption events can be detected and weighed. The remaining 70% of the events occur without participants interacting with the scale or when noisy interactions with the scale prevent weight measurement. The relationship between bite …

Evaluation Of A Mycorrhizal-Like Fungus, Piriformospora Indica, On Floriculture Crops, Allison Justice

All Dissertations

Piriformospora indica is a fungal endophyte, often called an arbuscular mycorrhizal-like fungus, that has been shown to provide benefits to plant symbionts by increasing nutrient uptake, biomass production, flower number, and disease resistance in a wide range of plant hosts. Research was carried out to investigate the ability of P. indica to improve plant production in floriculture crops. The first objective was to determine the optimal environmental conditions for growing P. indica in pure culture. Environmental conditions were optimized to produce the maximum chlamydospores for inoculum preparation (Chapter 1). These findings were used in the remaining chapters to prepare inocula …

Heterotrophic Culture Of Microalgae Using Biodiesel Derived Glycerol, Karthik Gopalakrishnan

All Dissertations

Algae-based technologies are fast growing and the growing demand for sustainable technologies is evident from the growing energy demand and global warming. Microalgae culturing for higher lipid contents have been a hot topic of intense discussion in the past years. Crude glycerol, a byproduct from biodiesel production seems to be an attractive feedstock for microbial cultivation and crude glycerol has been proven as a good alternative feedstock for cultivation of Chlorella protothecoides. The effect of impurities present in the crude glycerol is important to develop a method for high-density cultivation of microalgae to increase the commercialization potential of algae systems. …

Platform Technologies To Advance Clinically Relevant Tissue Engineered Heart Valve Products, Leslie Neil Sierad

All Dissertations

Diseased heart valves are commonly replaced by mechanical, bioprosthetic, or allograft heart valves. These replacements provide major improvements in cardiac function and quality of life, but have significant limitations and eventually require surgical replacement within 15-20 years. These risks are particularly prominent in pediatric patients and young adults. The field of tissue engineering and regenerative medicine, which combines scaffolds and cells, holds great promise in developing living replacement heart valves that would self-repair and grow in size along with the growing children.

The long-term goal of this project is to generate living, tissue-engineered heart valves from biological scaffolds and autologous …

Clemson Commencement Program, May 2014, Clemson University

Clemson Commencement Programs

No abstract provided.

Analysis Of Volatile Compounds And Their Changes During Liquor Aging Of Chinese Liquor 'Gujing Gongjiu', Qingchuan Liu

All Theses

Chinese liquor 'Gujing Gongjiu' is a traditional Chinese distilled alcoholic beverage that is categorized into a strong-aromatic fragrance liquor. It has a strong mixed floral, fruity, and sweet flavor with a smooth taste. Volatile compounds were extracted by headspace solid-phase microextraction (HS-SPME) from Gujing liquors that were aged for 1, 5, 10, and 20 years. Optimization of HS-SPME method was performed to determine the best experimental condition for extraction of liquor volatiles. Gas chromatography-mass spectrometry (GC-MS) was used for quantitative and qualitative analysis of liquor volatiles. Volatile compounds were identified by Kovat's retention indices and their mass spectrum. The influence …

Avs Corner, May 2014, Kathy Still, Johanna Johnson, Allison Millican, Megan Kelley, Michelle Brauneis, Tina Rowland

AVS Corner

No abstract provided.

Social Learning And The Development Of Social Foraging Behavior In Golden Lion Tamarins (Leontopithecus Rosalia), Carly Slack

All Theses

Golden lion tamarins are highly social, group-living primates and are cooperative breeders. As such they are an ideal species in which to study social learning. Observations of six groups of wild golden lion tamarins were used to examine the development of social foraging and related behaviors in juveniles. Rates at which juveniles approached adults that were foraging for plant foods tended to decline with age (F=3.34, p=.0531), and the rates at which they begged (F=4.71, p=.0193), and foraged at the same site (i.e., co-foraged; F=3.44, p=.0495) decreased significantly. For prey foraging, rates at which adults vacated a site to allow …

An Examination Of The Feasiblity Of A Food Hub For The Pee Dee Region, Emily Purcell

All Theses

Horry County, the home of Myrtle Beach, South Carolina, attracts a large level of spending by visitors who are helping to fuel growing demand for locally produced food. Regional growers are interested in meeting this demand but are limited by retail requirements with respect to lot size, timing, and quality (including food safety aspects such as Good Agricultural Practices (GAP) certification). A number of communities have turned to food hubs as a means of aggregating production by local growers and providing appropriate marketing functions, such as storage and meeting standards. Hence, food hubs can fill a gap between producers and …

Comprehensive Cancer Care: An Outpatient Treatment Facility, Braden Abrams Reid

All Theses

A cancer diagnosis can result in a loss of decision-making and control for patients. The majority of clinical treatments fail to adequately address corollary symptoms and needs that arise both from treatment and from the disease itself. Thus, the architectural problem identified in this thesis inquiry is how can an outpatient cancer centre treat the whole patient comprehensively, while at the same time instilling control and re-empowering the patient? This thesis project proposes a patient-centered environment designed to support the myriad and nuanced needs of the cancer patient, as well as a site-specific proposal for a comprehensive outpatient cancer treatment …

Effect Of Low And High Molecular Weights Water Soluble Chitosan On Oxidation Of Whole Milk Powder, Wesam Al-Jeddawi

All Theses

Autoxidation significantly decreases the shelf life of whole milk powder due to primary and secondary oxidation products such as hydroperoxides, aldehydes, ketones, alcohols and hydrocarbons. Water soluble chitosan has been shown to reduce oxidation by chelating metals or combining with lipids resulting in a significant antioxidative effect. The objective of this study was to determine the antioxidative effects of different concentrations of low (L) and high (H) molecular weight water soluble chitosan (9 and 90 kDa, respectively) on whole milk powder (WMP). Commercially dried WMP was obtained and rehydrated 50% (wt/wt) with various aqueous chitosan solutions (2% L2, 4% L4, …

The Reference Transcriptome Of The Adult Female Biting Midge (Culicoides Sonorensis) And Differential Gene Expression Profiling During Teneral, Blood, And Sucrose Feeding Conditions, Dana Nayduch, Matthew B. Lee, Christopher Saski

Publications

Unlike other important vectors such as mosquitoes and sandflies, genetic and genomic tools for Culicoides biting midges are lacking, despite the fact that they vector a large number of arboviruses and other pathogens impacting humans and domestic animals world-wide. In North America, female Culicoides sonorensis midges are important vectors of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), orbiviruses that cause significant disease in livestock and wildlife. Libraries of tissue-specific transcripts expressed in response to feeding and oral orbivirus challenge in C. sonorensis have previously been reported, but extensive genome-wide expression profiling in the midge has not. Here, we …

Examining The Effects Of School-Level Variables On Elementary School Students' Academic Achievement: The Use Of Structural Equation Modeling, Matthew Robert Della Sala

All Dissertations

School finance scholars have called for the alignment of accountability policies with state finance formulae to allocate resources toward student learning goals (Adams, 2008; Ryan, 2008; Superfine, 2009; Verstegen, 2002). With the presence of accountability policies that focus on improving students’ academic achievement, state finance systems must be repurposed to allocate educational resources to schools based on research-based practices that are linked to student achievement. The purpose of this study is to test the sufficiency of a new conceptual model of the effects of educational resources on student achievement using structural equation modeling. The goal of this study is to …

Assessing The Bite Counter As A Weight Loss Tool, Michael Wilson

All Theses

Obesity is a growing health concern throughout the world. Health risks associated with obesity such as diabetes and heart disease result in obesity costing us over $170 million annually. Self-monitoring of Energy Intake (EI) is a critical element of a successful weight loss plan; however current methods to monitor EI are cumbersome and prone to under reporting. The primary purpose of the study was to develop and test an experimental diet protocol based on user feedback from the Bite Counter. A secondary purpose was to examine if this protocol would affect meaningful weight loss by device users. Data were collected …

Chemical Control Of Rootstock Regrowth In Grafted Watermelon And Its Effects On Plant Growth And Development, Shawna Daley

All Theses

High, year-round demand, increased production intensity without crop rotation, and the loss of Methyl Bromide as a soil fumigant, results in unfavorable soil conditions with increased soil pathogen populations for growing watermelon [Citrullus lanatus (Thunb) Matsum and Nakai]. Grafting watermelon onto resistant rootstocks provides successful control of soil-borne pathogens, and could increase yield and fruit quality; however, the high production costs, especially from labor, involved in grafted transplant production, has prevented the adoption of grafted watermelon use in the United States. Much of the labor is required for meristematic regrowth control, which can cause abortion of the scion and competition …

The Leconte Lodge: A Lens For The Evolution And Development Of The Great Smoky Mountains National Park, Lindsay D. Lanois

All Theses

Officially established in 1934, the Great Smoky Mountains National Park originated as part of a widespread cultural trend towards outdoor recreation, national tourism, and the federal government's assumption of responsibility for land conservation. However, the area of Tennessee and North Carolina selected by the federal government for the national park was not a purely unsettled landscape. Not only did the land serve as home to approximately ten permanent agricultural communities, but it also featured several hotels and lodges inspired by the burgeoning twentieth-century tourism industry. Beginning as a rudimentary hiking cabin constructed in 1925 and evolving throughout the 1930s, the …

An Empirical Study On Return To Education In Urban China, Jin Han

All Theses

This study uses the most recent household survey data from 2007 to estimate return to education in urban China. Most existing literature are based on the data from 1990s and early 2000s and find that the rate of return to education in urban China has experienced a rapid increase. This study investigates whether the return to education is still in an increasing trend in later 2000s and how the return to education changed in recent years. The estimates of return in this study are lower than the findings in early 2000s. The higher education expansion started in 1999 made unbalance …

Effect Of Bromelain On Duck Breast Meat Tenderization, Ahmet Buyukyavuz

All Theses

ABSTRACT This study was conducted to determine the effect of bromelain on duck breast meat tenderization. Duck breasts were marinated with different concentrations (0, 1.5, 3 and 4.5%; C2, B1.5, B3 and B4.5, respectively) of bromelain and a solution composed of 92% refrigerated water, 6% salt and 2% sodium tripolyphosphate by using a vacuum food tumbler machine. A second non-marinated control (C1) was also included. Marination tumbling was operated at 8 RPM for 8 minutes. Physico-chemical and quality parameters were determined on raw and cooked samples. A significant reduction (p<0.05) in pH was observed in all bromelain treated samples when compared to C2. Although there was a significant reduction in pH of B1.5 when compared to C1, there was no significant difference among B3, B4.5 and C1. A significant increase was observed in water holding capacity of B3 and B4.5 when compared to C1. While a significant reduction was observed in lightness (L*), no differences were observed in redness (a*) and yellowness (b*) values of marinated samples (C2, B1.5, B3 and B4.5) when compared to C1. However, a significant increase in lightness (L*), and a significant decrease in redness (a*) and yellowness (b*) were observed in cooked bromelain treated samples. No differences were observed in razor shear forces, moisture content and water activity between treatments. There was no significant difference in cook yield of all marinated samples (C2, B1.5, B3, and B4) when compared to the C1. A significant reduction (p<0.05) in Warner-Bratzler shear force values was observed in all marinated samples when compared to untreated control (C1). Based on results, use of bromelain in marination solutions under the conditions tested here would not be cost effective.

Comparison Of Simultaneous Distillation And Extraction (Sde) And Headspace Solid Phase Microextraction (Spme) For Determination Of Volatiles Of Muscadine Grapes (Vitis Rotundifolia), Pei-Ching Lin

All Theses

Vitis rotundifolia also called 'Muscadine' grows abundantly throughout the southeastern United States. Muscadine grapes have desirable flavors, making them suitable to be either consumed as fresh fruit or made into wine and jam. Moreover, muscadine grapes have been reported containing rich complex phytochemicals, including anthocyanins and polyphenols that possess antioxidant, anti-cancer, anti-inflammatory and antimicrobial activities. Therefore, muscadine grapes have become increasingly popular in recent years due to its unique aroma, as well as health benefits. Various volatile compounds contribute to the aromatic flavors of muscadine grapes. In regards of their low concentrations, conventional simultaneous distillation and extraction (SDE) and solid …

Mobile Interactive Fitness Technologies And The Recreational Experience Of Bicycling: A Phenomenological Exploration Of The Strava Community, William Smith

All Theses

Technology is now embedded in many aspects of daily life. Over the past few years, fitness related smartphone applications have steadily grown in popularity. Despite the prevalence of technology in recreation and leisure experiences, the topic remains to be thoroughly examined from a qualitative interpretive viewpoint. The purpose of this phenomenological study was to understand and describe how cyclists use and experience Strava--a GPS enabled smartphone application that tracks bicycle rides and uploads the data to an online community of other users. Particular attention was given to user motivations, affordances of the technology, and notions of community. After participant observation …

A Study Of Time-Based Features And Regularity Of Manipulation To Improve The Detection Of Eating Activity Periods During Free Living, Jose Reyes

All Theses

This thesis considers the problem of detecting when people eat by tracking their wrist motion. The goal of this work is to automatically detect the start and stop times of these eating activities. It builds upon previous work done by our research group, which developed an algorithm for automatically detecting peaks in activities associated with food preparation and clean-up. This peak detector is then used for segmenting data. These segments are then classified as eating or non-eating activities using a naive Bayes classifier based on probabilities obtained from computing different features in each segment. The original work introduced 4 features, …