Digital Commons Network^™

Optimization Of Modular, Long-Range, Ultra-Fast Optical Tweezers With Fluorescence Capabilities For Single-Molecule And Single-Cell Based Biophysical Measurements, Subash C. Godar

All Dissertations

An Optical tweezer is a tightly focused laser beam that applies and senses precise and localized optical force to a dielectric microsphere and offers a unique and effective tool for manipulating the single cell or cell components, including nucleotides and dynein motor proteins. Here, I used highly stabilized optomechanical components and ultra-sensitive detection modules to significantly improve the measurement capabilities over a wide range of temporal and spatial scales. I combined the optical tweezer-based force spectroscopy technique with fluorescence microscopy to develop an integrated high-resolution force-fluorescence system capable of measuring displacements at sub-nanometer, forces at sub-piconewton over a temporal range …

Performance Of Geotechnical Systems Under Extreme Hydroclimatic Events Using Finite Element Modeling, Tharshikka Vickneswaran

All Dissertations

Failure of geotechnical systems during hydroclimatic events such as heavy rainfall, flood, and drought often leads to significant socio-economic loss. The time-dependent stability and deformation behaviors of geotechnical systems subjected to hydroclimatic events must be investigated using a coupled flow-deformation formulation because the strength and deformation parameters vary with the degree of saturation and/or matric suction of the soil. Although the impacts of the hydroclimatic events on the geotechnical systems are assessed and summarized in the previous studies, all the analyses were carried out in a loosely coupled manner and/or uncoupled manner without considering the effects of water flow in …

Using Smart Phone Technology To Improve Daily Living Skills And Independent Living Skills For Individuals With Intellectual Disability, Jordan Stierle

All Dissertations

Independent living is an important facet of adult life. Individuals with ID often experience a combination of both intellectual and adaptive functioning deficits which negatively impact their ability to live independently. Fortunately, research has shown that assistive technology (AT), and particularly video prompting helps support independent living for individuals with ID. This single case multiple probe across participants design study investigated the efficacy of a highly customizable task analysis smartphone application in assisting three young adults with ID learn how to cook three different recipes. Findings indicate that all three participants experienced large effect size gains from using the AT …

Characterization Of Water Flow And Solute Transport Driven By Preferential Flow In Soil Vadose Zone, Abdullah Al Mamun

All Dissertations

The vadose zone acts as a buffer zone between the ground surface and the aquifers underneath and controls the transmission of infiltrating water and contaminants, for example, pesticides and chemical spills. Therefore, understanding the flow and transport processes that dominate the vadose zone is important. Macropores are ubiquitous and particularly found in abundance in the vadose zone. These macropores facilitate preferential flow, through which water travels rapidly deep into the soil, bypassing most of the porous matrix. Preferential flow and transport have environmental significance as their processes impact hydrology, ecology, agriculture, subsurface contamination, and waste management sectors. Thus, the overall …

Ecologies Of (Domestic) Trauma, Ecologies Of (Domestic) Violence: A Rhetorical Procession Toward Mourning, Charlotte E. Lucke

All Dissertations

In this dissertation, I posit that intimate partner violence is entrenched in an often-overlooked historical and rhetorical legacy of patriarchal cultural, structural, and direct violences. Many scholars in and outside of rhetorical studies have analyzed and critiqued public representations of trauma and violence, including intimate partner violence. Joining this conversation, I focus on the limitations in the ways influential rhetorical domains both represent and respond to people who abuse their intimate partners. Often, mass media represents people who abuse their intimate partners as individuals void of contexts. Similarly, the criminal justice system holds individuals responsible through law enforcement and incarceration. …

Effects Of Continuous In Situ Low-Dose Ionizing Radiation On Microorganisms, Molly E. Wintenberg

All Dissertations

Precise detection and monitoring of nuclear fuel cycle, enrichment, and weapon development activities are critical for supporting warfighter preparation in chemical, biological, radiological, nuclear, and explosives (CBRNE) operations, clandestine activities, and nuclear compliance. A biological sensing system could serve as an alternative to traditional detection methods by using organic material naturally present in the environment to discreetly detect residual trace nuclear material. Microorganisms provide an optimal platform for an alternative sensing system; however, their response to low levels of ionizing radiation is poorly characterized. Combining the power of next-generation sequencing and transcriptomic analysis, this dissertation takes an approach to obtain …

Valorization Of Biopolymers And Biomass To Produce Materials For A More Circular Economy, Moira Lauer

All Dissertations

With a globally increasing population and largely unchecked consumption of raw materials, human society is on track for devastating consequences. Two industries responsible for utilizing massive amounts of raw materials and generating equally gargantuan quantities of waste are the packaging and infrastructure sectors. In 2017 in Europe, for example, packaging reached a record 173 kg of packaging waste per capita. One of the largest packaging consumers is the food industry, in which 40% of packaging is made of petroleum-derived single-use plastic, leading to a massive carbon imbalance. “The Built Environment,” on the other hand, is responsible for about 50% of …

Crip Theory And Creative Briefs: Interpreting Disability In The Creative Process, Josh Loebner

All Dissertations

Brands, advertising agencies and other partners collaborate through curated phases to create marketing and advertisements, but within this process, how does the creative brief shape disability inclusion or exclusion? By moving the conversation from interpreting disability representation in an advertisement or finished marketing piece, to analyzing disability at the onset of the creative process, these insights present a critical contribution to scholarship on disability, marketing and consumer culture. Framed within crip theory, the research investigates how disability identity is—or is not—manifest in a creative brief within the creative process.

Much of marketing, advertising and disability studies scholarship centers on finished …

“I Can See The Forest For The Trees”: Examining Personality Traits With Trasformers, Alexander Moore

All Dissertations

Our understanding of Personality and its structure is rooted in linguistic studies operating under the assumptions made by the Lexical Hypothesis: personality characteristics that are important to a group of people will at some point be codified in their language, with the number of encoded representations of a personality characteristic indicating their importance. Qualitative and quantitative efforts in the dimension reduction of our lexicon throughout the mid-20^th century have played a vital role in the field’s eventual arrival at the widely accepted Five Factor Model (FFM). However, there are a number of presently unresolved conflicts regarding the breadth and …

How Transformative Leaders Break Down Barriers To Increase Access To Arts Integration For Black And Hispanic Students: A Multiple Case Study, Julia Mcclanahan

All Dissertations

The purpose of this study was to understand how transformative leaders break down barriers to increase access to arts integration for Black and Hispanic students. To fulfill this purpose, I conducted a multiple case study of two school sites in two different school districts. I collected the data for the cases through interviews with the principal of each school, interviews with two teachers from each site, field notes from site visits, and documents and artifacts from each site. This study is significant because the data provides transformative leaders with an action plan in order to make arts-integrated programs more equitable …

Nonviral Approaches For Delivery Of Crispr-Cas9 Into Hepatocytes For Treatment Of Inherited Metabolic Disease, Tanner R. Rathbone

All Dissertations

Inherited metabolic diseases (IMDs) of the liver have a collective occurrence of about 1 in 800 births. Current therapies for IMDs of the liver are limited, and the only curative option for patients is orthotopic liver transplantation. Due to shortages of organ donors, immunosuppression following the transplantation, and mortality risks associated with the procedure, alternative treatment options are necessary for curing IMDs of the liver. Therapeutic gene editing has been proposed as a potential strategy for treating IMDs of the liver. Adeno-associated viral vectors (AAVs) are the most widely used delivery method for liver-targeted gene therapies. Although widely used for …

Homestays In Nepal In The Era Of Covid-19, Anup K C

All Dissertations

Homestays are accommodation facilities provided by hosts in their own residence. Usually, guests are provided food and lodging by the host family members. These tourism facilities are a source of alternative income in rural areas of countries like Nepal. But now, COVID-19 has adversely affected homestays along with other tourism sectors, so it is necessary to assess homestays in Nepal in the COVID-19 era. For this dissertation, online interviews were conducted with homestay stakeholders (54 homestay operators, 12 homestay leaders, 11 homestay experts, and 8 biodiversity experts). These online interviews were recorded in the Nepali language and were translated and …

Developing Implantable Hydrogel-Based Sensors To Measure Biomarkers In Synovial Joint Fluid Using Plain Radiography, Uthpala N. Wijayaratna

All Dissertations

The goal of my dissertation research is to develop implantable sensors that attach to prosthesis prior to implantation and measures biomarkers of infection in joint fluid in order to detect, monitor, and study infection using plain radiography. Joint replacement surgeries are common procedures improving the mobility and lives of millions of people worldwide. Although the surgeries are generally successful, about 1% of prosthetic hips become infected. If the infections are not detected and treated promptly with antibiotics and surgical debridement, device removal is almost always required to treat the infections. Therefore, it’s important to detect post-surgery infections early and monitor …

Walking Back The System Trope: Reimagining Incarceration And The State Through A Spatial Theory Approach, Cody Hunter

All Dissertations

This dissertation critiques the systems theory approach to incarceration policy, practice, and research and proposes a rhetorically informed spatial theory approach as an alternative. Offering a non-hierarchical complexity theory as a bridge between systems and space, I then integrate rhetorical listening as a strategy for navigating and operationalizing our proposed spatial theory approach. I then apply our proposed methodology to archival research, focusing on the South Carolina Penitentiary as a case study, and offer two heuretic experiments to explore the range of this methodology for archival research. I also explore potential applications of this rhetorically informed spatial theory approach in …

Allegories And Alleles: A Narrative Policy Framework Analysis Of The Debate Over Animal Bioengineering, Betsy Presgraves

All Dissertations

With the USDA’s recent takeover of animal bioengineering regulation, public comments have been reopened about the issue, with the Biden administration seeking more information about public opinion. The debate over U.S. regulation has raged for decades with a pro-genetic modification coalition seeking more efficient approvals and the anti-genetic modification coalition seeking labelling, more open and strict approval processes, and even outright bans. Each side has employed a variety of communication approaches, chief among them the use of narrative.

This study aims to determine how narrative structures affect this debate. Using the Narrative Policy Framework (NPF), I seek to examine this …

Narrating Madness: Building Narratives Against Privileged Identity, Kristin Santa Maria

All Dissertations

This dissertation examines how competing narratives related to madness and mental health can provide insight into the inconsistencies of preconceived biases that tie into privilege and power. These biases relate to identities associated with race, gender, class, and embodiment. By exploring madness narratives, we can see how madness often becomes a quality used to isolate and “other” people that act against typical societal and cultural norms. Works of fiction and nonfiction that pertain to madness narratives can either be used to perpetuate stereotypes of normalcy or as a vehicle to allow for a more open and frank discussion of madness …

Coding Christianity: Negotiating Religious Dialogue In Online Participatory Spaces, Shauna Chung

All Dissertations

This dissertation examines rhetorical conditions and internet-mediated communication strategies that open and close dialogue between individuals with diverse and conflicting worldviews. The author illustrates this tension through sacred-secular interactions in college composition classrooms and online environments, positing that navigating conflict between these discourses—namely those espoused by religiously committed students and public university instructors—often requires stepping outside of adversarial communication frameworks. This project makes a case for models of civic engagement that use more deliberative rhetorical approaches prioritizing empathy over defensiveness and understanding before persuasion. To develop these non-adversarial communication approaches for the composition classroom, the author looks to participatory media …

Queering The Outdoors: Lgbtq2sia+ Identity In Outdoor Recreation, Sandra Heath

All Dissertations

Objective. The purpose of this project was to learn about the lived experiences of outdoor professionals who identify as Lesbian, Gay, Bisexual, Transgender, Queer, Two Spirited, Pansexual, Intersex, Asexual, Plus (LGBTQ2SIA+ or LGBTQ+). In this study, we are gifted with stories that delve into queer identity in the outdoors, with the understanding that not all LGBTQ+ individuals experience the outdoors in the same way. Specifically, this study considers these experiences across temporal dimension of childhood and adulthood, the defining event(s) of coming out, the role of community, and reflections on outdoor recreation professions. Methods. A critical narrative approach was used …

Sustainable Composites From Agricultural And Petroleum Waste, Menisha S. Karunarathna

All Dissertations

Green gas emission has been a pervasive and persistent subject of debate for a prolonged period. The soaring number of industries and vehicle fuel emissions presage a concomitant rise in global CO₂ emissions. Global cement production is responsible for 8% of the total CO₂ release, yet, the production continues due to the surging demand. Hence, there is an ongoing quest to find alternatives for cement and building materials produced with zero to lower CO₂ emissions. The work presented in this dissertation focuses on finding recyclable, zero CO₂ gas-producing high sulfur biocomposites materials, which can compete with …

Cell Tracking At Low Frame Rate Using Deep Learning And Bayesian Integration, Xiang Zhang

All Dissertations

Tracking cells over time is a fundamental task in live-cell imaging, and often requires costly manual analysis if images are not acquired with high enough frame rate. Acquiring high frame rate images, however, can limit the number of conditions explored and cells analyzed, and contribute to photobleaching, which makes fluorophores dimmer and phototoxicity, which affects cell health and renders the resulting data unusable.

Assuming a relatively high frame rate in image acquisition, state-of-the-art cell tracking approaches rely on either spatial proximity or morphological similarity to link cells in consecutive frames. The problem is that, at low frame rate, both approaches …

Graphene Oxide-Thermoplastic Nanocomposites: Fabrication And Properties, Seyedeh Mastooreh Seyedi Ghezghapan

All Dissertations

Polymer nanocomposites are used for a wide variety of applications. These nanocomposites can have a number of important characteristics depending on the nature of the nanomaterial, its size, volume fraction, its distribution, and interactions within the host polymer. Mechanical strength, thermal and electrical conductivity are some of these materials' most focused and studied features. Besides the positive influences of the reinforcements, nanomaterials also might have some adverse impacts on the polymer matrix. These issues could arise from the aggregation of the fillers and the poor interfacial interactions of the components. Several approaches are introduced to modify the nanofillers and enhance …

Development And Testing Of The Mueller Assessment Of Transition (Mat): A Tool For Measuring Older Adults' Wellbeing When Transitioning Into Assisted Living Facilities, Kaitlin Mueller

All Dissertations

Transitions into assisted living facilities (ALFs) may influence older adults’ wellbeing. Positive influences on older adults’ wellbeing are referred to as adjustment strategies, whereas negative influences are called constraints to wellbeing. Theoretical underpinnings for these influences are found in the Hierarchical Leisure Constraints Theory and the Transition Process Framework. Past research indicates a few influences on older adults’ wellbeing, such as family relationships, perceived physical health, and connections to the community. However, no assessment existed to quantify their wellbeing when relocating into an ALF. Therefore, this dissertation details the development and factor structure of the Mueller Assessment of Transition (MAT), …

About The Contributors

The Robert Frost Review

No abstract provided.

Editor's Note, Virginia Smith

The Robert Frost Review

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Table Of Contents

The Robert Frost Review

No abstract provided.

Google Translate And Biliterate Composing: Second-Graders' Use Of Digital Translation Tools To Support Bilingual Writing, Lindsey Rowe

Publications

Research is needed exploring how teachers and students in English-medium schools transform classrooms to welcome and value bilingualism and biliteracy. This article draws on social literacies and placed resources perspectives to explore how one classroom of second-grade students used Google Translate as a tool to support their biliterate composing. Constant comparative analyses revealed three patterns related to students' use of this tool. Students used Google Translate to support their inclusion of bilingual text and to interact with peers. They also negotiated limitations around the use of Google Translate. Examples of each pattern are used to illustrate how students interactionally engaged …

Postcards And Chemistry Books: Immigrant Detention Literacies, Stephanie M. Madison, Mikel W. Cole

Publications

No abstract provided.

Clemson Chronicle, Spring 2022, Clemson University

Clemson Chronicle

Clemson University's twice-yearly literary magazine, featuring creative prose, poetry, features, art and photography.

The Professional Constructor, Full Issue, Volume 47, Number 01

The Professional Constructor

No abstract provided.

Using Knowledgeable Agents Of The Digital And Data Feminism To Uncover Social Identities In The #Blackgirlmagic Twitter Community, Golnaz Arastoopour Irgens

Publications

Online spaces have the capacity to be powerful informal learning and identity development spaces for marginalized communities. However, there is still much work to be done to uncover these complex social identities using ethical big data analyses. In this study, I draw on the theory of Knowledgeable Agents of the Digital, data feminism, and critical reflexivity practices to engage with a #blackgirlmagic Twitter dataset from 2016 to 2019. Using Epistemic Network Analysis, findings suggest that the #blackgirlmagic community self-defined their social identities around Black beauty, academic/professional accomplishments, and social justice. Because the women and girls of #blackgirlmagic were agentive in …