Digital Commons Network^™

Using In Vitro, In Silico, And In-Classroom Techniques To Address The Gender Data Gap In Health Care, Kelsey Watts

All Dissertations

Cardiovascular diseases (CVDs) are the leading cause of death worldwide in males (XY) and females (XX). Prior to menopause, females have a relative protection against serious cardiac pathologies compared to age-matched males. This phenomenon is widely attributed to the ovarian hormone estrogen. Unfortunately, hormone replacement therapy to maintain estrogen levels in postmenopausal females has overall adverse effects, and it is not recommended for long-term use or as a preventative measure for eCVDs. A major driver of CVDs, specifically heart failure, is cardiac fibrosis: the continued buildup of scar tissue that reduces the heart’s ability to pump. There are currently no …

Isolation And Characterization Of Extracellular Vesicles From Various Biological Matrices Using Capillary-Channeled Polymer (C-Cp) Fiber Solid-Phase Extraction Spin-Down Tips, Kaylan Jackson

All Dissertations

A number of recent works have emphasized the need to isolate nanometer-scale analytes, like extracellular vesicles (EVs), from various biologically-relevant fluids. Exosomes are a subset of small EVs that range from 30-200 nm in diameter that serve as biomolecular snapshots of their cell of origin containing mother cell-specific DNA, miRNA, mRNA, and proteins. As critical components of intercellular communication, exosomes and other EVs play significant roles in many physiological and pathological processes. Diverse populations of these vesicles can be collected from biofluids, including blood, saliva, and urine, from cell culture conditioned media and primary cells, and even from plant …

An Intergenerational Study Of The Entrepreneurial Nature Of Agritourism Operators, Will Culler

All Dissertations

Economic and non-economic trends have left farm operators of all ages contemplating enterprise diversification strategies to create advantages and to ensure their farms' sustainability for future generations. One such strategy is agritourism, in which a visitor to a working farm or other agricultural setting interacts with the farm landscape or participates in an agricultural process for tourism or leisure purposes. This study aims to contribute to academics, researchers, extension educators, practitioners, and farm service providers who offer training and resources to better equip current and future agritourism operators. The study tested the general hypothesis that agritourism operators' entrepreneurial goals and …

Modeling Electrostatics In Molecular Biology And Its Relevance With Molecular Mechanisms Of Diseases, Mahesh Koirala

All Dissertations

Electrostatics plays an essential role in molecular biology. Modeling electrostatics in molecular biology is complicated due to the water phase, mobile ions, and irregularly shaped inhomogeneous biological macromolecules. This dissertation presents the popular DelPhi package that solves PBE and delivers the electrostatic potential distribution of biomolecules. We used the newly developed DelPhiForce steered Molecular Dynamics (DFMD) approach to model the binding of barstar to barnase and demonstrated that the first-principles method could also model the binding. This dissertation also reflects the use of existing computational approaches to model the effects of Single Amino Acid Variations (SAVs) to reveal molecular mechanisms …

Inventing Network Composition: Mobilizing Rhetorical Invention And Social Media For Digital Pedagogy, Jacob Richter

All Dissertations

Inventing Network Composition: Mobilizing Rhetorical Invention and Social Media for Digital Pedagogy investigates how students learn through writing and invention in digital social networks. Pursuing a primary research question of How do student composers invent within networked social media environments?, the dissertation examines how social media and digital writing tools can help students to learn, connect, and share generatively. The core theoretical contribution that this dissertation offers is a theory of network composition, which is a mode of invention that composers engage in social media environments that is intensely social, that is structured by a digital interface, that …

Evaluating Privacy Adaptation Presentation Methods To Support Social Media Users In Their Privacy-Related Decision-Making Process, Moses Namara

All Dissertations

Several privacy scholars have advocated for user-tailored privacy (UTP). A privacy-enhancing adaptive privacy approach to help reconcile users' lack of awareness, privacy management skills and motivation to use available platform privacy features with their need for personalized privacy support in alignment with their privacy preferences. The idea behind UTP is to measure users' privacy characteristics and behaviors, use these measurements to create a personalized model of the user's privacy preferences, and then provide adaptive support to the user in navigating and engaging with the available privacy settings---or even implement certain settings automatically on the user's behalf. To this end, most …

Development Of Plasmonic And X-Ray Luminescence Nanoparticles For Bioimaging And Sensing Applications, Meenakshi Ranasinghe

All Dissertations

This dissertation discusses the development of plasmonic and X-ray luminescence nanoparticles (~100 nm) to use in bioimaging and sensing applications. The nanoparticles have interesting optical properties compared to their atomic levels and bulk materials. The optical properties of nanomaterials can be controlled by changing size, shape, crystal structure, etc. Also, they have a large surface area that can be functionalized with biomolecules. Therefore, the optical properties and biofunctionalized nanomaterials are useful in biomedical applications such as targeted drug delivery, bioimaging, and sensing. The overall theme is to use nanoparticles with interesting optical properties compared to their atomic levels and bulk …

Reading Toward Breath: A Poetic Ecology Of Creative Reading, Jessica Schad Manuel

All Dissertations

Reading is an act of perception that begins in wonder and leads to wisdom. It is not a response to writing but a response-ability we have to interact with the world around us and the phenomena before us. Reading with the body leads to wisdom, and when we participate in our existence by being in the world, we are reading. So what is reading? Reading is making. We make connections, and we form relationships. The act of reading is more than a cognitive process and even surpasses the phenomenological encounter.

My research describes the reader’s relationship to the text as …

Network Of Folx: Applying The Use Of Community Cultural Wealth To The Experiences Of Black First-Generation Hbcu Students, Maurice Williams Jr

All Dissertations

Despite improvements in the rates of college admission over the past few decades, college persistence and graduation rates continue to be problematic, particularly for marginalized students—students of color and students from low-income and/or first-generation families at all institutional types. When attention is shifted to Black first-generation students at Historically Black Colleges and Universities (HBCUs), persistence research neglects to include how Black first-generation college students own their educational experience to gain access to college and persist through graduation nor does the research examine cultural factors that help these students persist through the college-going process. Building from both student persistence and community …

Use Of A Trial Function Method To Semi-Analytically Simulate Matrix Diffusion In Heterogeneous And Fractured Media, Kien Trung Pham

All Dissertations

A semi-analytic trial function model is applied to simulate matrix diffusion in systems of parallel fractures, 2-D/3-D discrete fracture networks, and in 3-D heterogeneous media. The trial function model gives low normalized root mean square error (NRMSE) when compared to the parallel fracture analytical solution over a range of fracture spacing, with considerations for retardation and decay. The semi-analytic trial function model is efficient in time and maintains less than 6% NRMSE when it simulates matrix diffusive transport in 2-D/3-D discrete fracture networks (DFN). The model can predict DFN plume within a few meters of accuracy compared to fine-grid DFN …

Spaces For Whom? : Identifying Participatory Strategies To Foster Students' Sense Of Belonging On Community College Campuses, Cynthia Clegg Davies

All Dissertations

In the United States, community colleges have served diverse student populations, including students of color and students with disabilities. While these colleges are celebrated for their access and affordability, student success is not guaranteed. As educators work to continuously improve course and program completion, students’ sense of belonging is critical. However, a review of scholarship from education, architecture, and planning revealed how students have navigated campuses that have not met their needs or reflected their experiences and have even been settings for discriminatory behaviors ranging from microaggressions to oppression. Recognizing the potential to increase sense of belonging through student participation …

Heat Stress Response And Excystation In Entamoeba Histolytica, Irem Bastuzel

All Dissertations

Entamoeba histolytica is a water- and food-borne intestinal protozoan parasite that causes amoebiasis and liver abscess and is responsible for symptomatic disease in approximately 100 million people each year leading to ~ 100,000 deaths. The most common disease transmission follows the oral-fecal route, but it can also be transmitted by mechanical vectors such as animals carrying the amoeba from contaminated sources to water systems. In rare cases, disease transmission has been recorded in some patients in which men-to-men sexual practices were preferred.

The life cycle of E. histolytica starts through ingestion of infectious cysts, which are non-dividing, quadri-nucleated structures surrounded …

Understanding Vascular Pathologies In Diabetes: Optimizing Tissue Engineered Blood Vessels Resistant To Diabetic Complications, Bethany Lefeber

All Dissertations

Diabetes Mellitus, a major risk factor for cardiovascular disease, currently affects 463 million people, or 9.3% of the world’s population – with numbers estimated to reach 578 million people by 2030. Hyperglycemia, dyslipidemia, oxidative stress, and inflammation, major manifestations of diabetes, together, significantly increase the risk of vascular tissue damage. Vascular damage is primarily caused by the interaction of cells and extracellular matrix (ECM) components with the elevated levels of glucose and lipids circulating through the blood and in the ECM. ECM proteins, such as elastin and collagen, are subject to glycation and crosslinking via advanced glycation end products (AGE) …

Examining Language Ideologies And Trilingual Practices In Family, Community, And School Contexts: Three Case Studies, Mihaela Gazioglu

All Dissertations

Despite increasing knowledge on trilingualism, we still have a lot to learn about the intricacies of becoming trilingual and what people do with different languages that they use or are exposed to (Chevalier et al., 2012; Choi, 2019; Cruz-Ferreira, 2006). This three-study dissertation employed qualitative research methods to address three aspects that current research offers little information. Each study focused, respectively, on 1) a child’s and her parents’ daily language practices to construct meaning in three languages, 2) how mothers' language ideologies influence their language practices and the support they offer to their trilingual children, and 3) teachers’ language ideologies …

Microbial, Chemical, And Functional Components In Kefir, Natto, And Feed Ingredients, Dana Mccurdy

All Dissertations

Kefir grains were purchased from online, home kitchen vendors in the United States (n = 22), inoculated into UHT milk, and incubated at 25°C for 22 hours. Results indicated lactobacilli, lactococci and yeast in all samples. Six out of 11 samples contained coliform, and all samples contained pseudomonal and staphylococcal species. Using targeted genome sequencing (16S) of kefir grain samples 1, 2, and 3 revealed that lactobacilli were the predominant genera of bacteria. ITS sequencing revealed different fungal species in each grain. Results indicated potential presence of menaquinone-4 and menaquinone-7 by UPLC-MS/MS analysis.

The fermentation of whole and ground soybeans, …

Evaluation Of Life Cycle Costs, Benefits, And Public Perceptions Of Greywater Reuse Systems For Supplementing Conventional Water Delivery, Sreeganesh Reddy Yerri

All Dissertations

The water utility industry is under enormous pressure to meet the challenges of increasing demands due to population growth, lifestyle changes, and depleting freshwater resources. The current and predicted future deficit scenarios challenge water supply managers to come up with a sustainable and reliable alternative source while making the supply infrastructure smarter and resilient. One such alternative source is the greywater that is available at the point of consumption itself. With certain limitations, there have been studies performed to evaluate the life cycle costs and expected monetary benefits of decentralized greywater reuse systems, but the public and health bureaus are …

Feasibility Of Using Ultra-High-Performance Concrete (Uhpc) As A Repair Material Durability Performance, Rebar Coating, And Bond Characterization, Haitham Hussein

All Dissertations

Ultra-High Performance Concrete can be a very good material for use in repair works. The ability of chloride ions to pass through repaired concrete layers is suppressed if an impermeable concrete like UHPC is used. However, thickness of the UHPC layer and the orientation of the repair layer relative to the direction of chloride ingress may influence its effectiveness. This study is an attempt to evaluate the effect of varying thicknesses and cross sections areas of UHPC over a precast concrete and the direction of the UHPC layers relative to the direction of chloride ion movement on the resistance offered …

Computational Characterization Of Nonwoven Fibrous Materials: Transport And Wetting Properties, Fang Wang

All Dissertations

Nonwoven fibrous materials represent a platform of flexible material substrates. Nonwovens are widely used in the production of napkins, paper, filters, wound covers and face masks. In addition, for many applications, nonwoven materials interact with fluids. For example, in filtration applications, nonwoven materials are used to clean fluids containing solid particles or emulsified droplets. The filtration performance is affected not only by the geometrical arrangement of fibers in non-woven materials but also wettability of fibers. Understanding the transport properties of nonwoven materials and interactions between the dispersed droplets and solid substrate is crucial for the design and optimization of filter …

Radioluminescence Based Biochemical Sensing And Imaging Strategies To Measure Local Drug Release And Ph, Gretchen B. Schober

All Dissertations

In this dissertation we describe methods for measuring infection relevant biochemical analytes using radioluminescent and ultrasound luminescent materials. Films and nanoparticles fabricated with europium doped gadolinium oxysulfide (Gd₂O₂S:Eu³⁺) are used to quantitatively measure radiolabeled pharmaceutical concentration, specifically tritium labeled vancomycin (³H-vancomycin). Europium and dysprosium doped strontium aluminate is used to fabricate an ultrasound modulated, pH sensing film. These methods are indicated for theranostic evaluation of implant associated infection. Bacterial biofilms are inherently resistant to traditional antibiotic treatment and can coat biomedical implants. These biofilm related infections are difficult or impossible to eradicate …

Development Of A Reverse Engineered, Parameterized, And Structurally Validated Computational Model To Identify Design Parameters That Influence American Football Faceguard Performance, William Ferriell

All Dissertations

Traumatic brain injury (TBI) continues to have the greatest incidence among athletes participating in American football. The headgear design research community has focused on developing accurate computational and experimental analysis techniques to better assess the ability of headgear technology to attenuate impacts and protect athletes from TBI. Despite efforts to innovate the headgear system, minimal progress has been made to innovate the faceguard. Although the faceguard is not the primary component of the headgear system that contributes to impact attenuation, faceguard performance metrics, such as weight, structural stiffness, and visual field occlusions, have been linked to athlete safety. To improve …

Design And Operation Of A Microwave Flow Cytometer For Single Cell Detection And Identification, Jeffrey A. Osterberg

All Dissertations

Microwave dielectric sensing has become a popular technique in biological cell sensing for its potential in online, label-free, and real-time sensing. At microwave frequencies probing signals are sensitive to intracellular properties since they are able to penetrate cell membranes, making microwave flow cytometry a promising technology for label-free biosensing. In this dissertation a microwave flow cytometer is designed and used to measure single biological cells and micro particles. A radio frequency (RF)/microwave interferometer serves as the measurement system for its high sensitivity and tunability and we show that a two-stage interferometer can achieve up to 20 times higher sensitivity than …

Rewilding With Ar And Vr: Facilitating Care With Photography In Physically Immersive Apps, Kailan Sindelar

All Dissertations

In this dissertation I analyze two AR apps from Internet of Elephants, Safari Central and Wildeverse, and one VR app from National Geographic, National Geographic: Explore VR. These three apps use photography as the central tool for engagement, attempt to educate users, and prompt them to care about wildlife and wilderness. However, the ethical consideration of design has largely ignored representations of the environment, especially as it may intersect with facilitating care for wilderness and wildlife that is experiencing the effects of habitat destruction and environmental degradation. This project begins developing a critical discussion of how wilderness and …

Revealing The Role Of Electrostatics In Molecular Recognition, Ion Binding And Ph-Dependent Phenomena, Mihiri Hewa Bosthanthirige

All Dissertations

In this dissertation, we study the role of electrostatics in molecular recognition, ion binding and pH-dependent phenomena. In this work that includes three different research projects, the Poisson-Boltzmann (PB) model is used to describe the biological system and Delphi (which is a popular tool for solving the PB equation (PBE)) to study the electrostatics of biomolecular systems.

Chapter two aims to investigate the role of electrostatic forces in molecular recognition. We calculated electrostatic forces between binding partners separated at various distances. To accomplish this goal, we developed a method to find an appropriate direction to move one chain of protein …

Mitral Valve Tissue Engineering - A Dynamic Model For Investigating The Mechanism Of Valvular Pathology, Collin Owens

All Dissertations

Heart valve disease affects an average of 2.5% of the population in the United States. The mitral valve (MV) is the most complex of the heart’s four valves and is most associated with the disease by exhibiting altered extracellular matrix (ECM) which translates into stenosis or regurgitation. These diseases are typically degenerative in nature and can be accelerated by risk factors such as diabetes and hypertension. With diabetes and hypertension affecting 425 million and 1.39 billion people worldwide, further investigation into these risk factors is warranted. This study aims to develop and test an in vitro model of MV disease. …

Selected Female High School Athletic Directors Describe Their Career Trajectories, Mary Helen Coleman Martin

All Dissertations

ABSTRACT

There is an underrepresentation of women in the formal role of athletic directors in the interscholastic sport setting. Additionally, little is known about the lack of female representation at the interscholastic level. Utilizing phenomenological methodology, this study explored the career paths and lived experiences of interscholastic athletic directors. Using purposeful sampling, 13 female interscholastic athletic directors took part in semi-structured interviews. Emerging ideas from the lens of current female interscholastic athletic directors offer implications about direction and information needed to assist inspiring young females as they ascend the career ladder in becoming interscholastic athletic directors.

Clemson University Board Of Trustees, Full Minutes, 2022 July 22, Clemson University Board Of Trustees

Minutes

No abstract provided.

Compositional And Functional Changes In Microbial Communities Of Composts Due To The Composting-Related Factors And The Presence Of Listeria Monocytogenes, Hongye Wang, Vijay Shankar, Xiuping Jiang

Open Access Publishing Fund

Listeria monocytogenes is a leading foodborne pathogen that can contaminate fresh produce in farm environment, resulting in deadly outbreaks. Composts contain a diversity of microorganisms, and some of them may be compost-adapted competitive exclusion microorganisms against L. monocytogenes. To understand interactions between compost microflora and the pathogen, both dairy- and poultry-wastes based composts (n = 12) were inoculated with L. monocytogenes, and then analyzed by next-generation sequencing approaches along with culturing methods. DNA extraction and enumeration of L. monocytogenes were performed at 0 and 72 h post-incubation at room temperature. The major bacterial phyla were identified as Firmicutes (23%), Proteobacteria …

Ptrc Customer Assessment Survey And Best Practices, John Schlipp, Christian Sheehy

Journal of the Patent and Trademark Resource Center Association

Assessment of customer activities in academic, public, and special libraries demonstrates their impact and value to library administrators and constituents. In turn, this assists in securing and maintaining advocacy and financial support. Although many formal and informal assessment best practices have evolved to measure the quantitative and qualitative impact of information literacy instruction, few if any best practices have been established to measure impact specific to Patent & Trademark Resource Center (PTRC) customers. This article reviews the results of a survey of PTRC Libraries and their customer assessment practices. Such analysis reveals best practices for other PTRCs to build upon …

Fair, Equitable, And Just: A Socio-Technical Approach To Online Safety, Daricia Wilkinson

All Dissertations

Socio-technical systems have been revolutionary in reshaping how people maintain relationships, learn about new opportunities, engage in meaningful discourse, and even express grief and frustrations. At the same time, these systems have been central in the proliferation of harmful behaviors online as internet users are confronted with serious and pervasive threats at alarming rates. Although researchers and companies have attempted to develop tools to mitigate threats, the perception of dominant (often Western) frameworks as the standard for the implementation of safety mechanisms fails to account for imbalances, inequalities, and injustices in non-Western civilizations like the Caribbean. Therefore, in this dissertation …

Public Service Interpreter Education In The Gulf States: Ideas For Curriculum Design And Teaching, Mustapha Taibi

International Journal of Interpreter Education

The Gulf States host large numbers of non-Arabic-speaking residents and visitors. These non-nationals need to deal with such public services as hospitals, schools, courts, and other local administrations. In many cases, English is used as a lingua franca; however, not all public service staff or clients are able to speak or communicate effectively in this language. The communication needs in such situations require the assistance of professional public service interpreters, which, in turn, calls for appropriate education. In this paper, I outline education needs in public service interpreting in the Gulf States; provide an overview of common curricular contents and …