Digital Commons Network^™

Optimizing Crispr/Cas9-Mediated Knockdown Of Angptl3 In Liver Cell Lines And Mouse Hepatocytes, Meredith Reeves

All Theses

Familial hypercholesterolemia (FH) is a genetic condition characterized by elevated levels of low-density lipoprotein cholesterol (LDL-C) that leads to an increased risk of developing cardiac disease early in life (Shah et al., 2020). Current treatments such as statins and PCSK9 inhibitors have helped lower LDL-C levels, however they require repeated administration every 4-6 weeks to remain effective (Raal et al., 2018). Angiopoietin-like 3 (ANGPTL3) is an inhibitor of plasma lipid metabolism that has become a promising molecular target for the treatment of FH. Individuals with non-functional copies of ANGPTL3 demonstrate low levels of plasma LDL-C and triglycerides, indicating a protective …

“We’Re Not Gay One Month Out Of The Year…We’Re Always Who We Are.” Exploring Connections Between Organizations, Pride Branding, And Lgbtq+ Publics, Sam Goodman

All Theses

LGBTQ+ visibility has increased in American society; moral acceptability of these identities increased significantly over the last 20 years alone and several US Presidents deemed June to be Pride Month in recognition and celebration of these individuals. To major companies and organizations, LGBTQ+ identities – which constitute more than 11 million US adults – are a growing and richly diverse market segment with considerable buying power and cultural influence. As companies attempt to engage with this segment, they increasingly attempt to engage with Pride Month and events related to Pride – including but not limited to having same-sex representation in …

Modeling Hierarchical Porous Electrodes With Tailored Anisotropic Structure, Debanjan Sarker

All Theses

Modern electric vehicles and consumer electronics applications demand high specific energy from Li-ion batteries, which can be charged and discharged faster. In the search for high specific energy, researchers have tried to make thicker battery electrodes which contain a greater proportion of active material. While chemical and structural modification of electrodes can help to increase the electronic conductivity of active material, the microstructure of porous electrodes can be engineered to enhance ion transport for fast charge and discharge. Previous research has shown that macropores introduced by directional freeze tape casting can enhance the performance of thick porous electrodes. For instance, …

Distribution And Management Of Phytophthora Species On Lavender In The United States, Daniel Dlugos

All Dissertations

Phytophthora root and crown rot (PRCR) is currently the most important disease on lavender (Lavandula spp.) in the United States. The disease was first described on English lavender (L. angustifolia) in a Maryland nursery in 1991, with Phytophthora nicotianae as the causal agent. Since that time, the disease has been reported on multiple continents and as caused by several species of Phytophthora. This study examined the distribution and pathogenicity of Phytophthora species on lavender in the United States, requested lavender grower feedback regarding their production and concerns, and examined efficacy of selected management options. Lavender …

Understanding Dynamics Of Polymers Under Confinement: A Molecular Dynamics And Neutron Scattering Study, Supun Samindra Kamkanam Mohottalalage

All Dissertations

The current study probes the structure, dynamics, and rheological behavior of associating polymers including ionomers in melts and solutions as well as conjugated polymers confined into nanoparticles, using molecular dynamics (MD) simulations and neutron scattering techniques. The study focuses on two families of associative polymers, ion containing macromolecules and conjugated polymers.

Polymers that consist of ionizable groups along their backbone found uses in a broad range of applications. Examples include light weight energy storage and generation systems, and biomedical applications, where the polymers act as ion exchange membranes, and actuators. The ionic groups tend to form clusters that are in …

Isolation Of Proteins And Extracellular Vesicles Via Capillary-Channeled Polymer Fiber Stationary Phases, Lacey Billotto

All Theses

Capillary-channeled polymer (C-CP) fiber stationary phases have been developed by the Marcus lab as an alternative approach to traditional, commercially-available silica-based supports for biomacromolecule separations. C-CP columns are created with natural and synthetic base polymers in a variety of chemistries and shapes, allowing for customization of separations, while providing low material costs and ease of construction. The stationary phase is virtually nonporous with respect to the size of proteins and can operate under high linear velocities (~100 mm s^-1) while maintaining relatively low backpressures. As a result of these hydrodynamic benefits, low flow resistance at high volume flow …

The Digital Reconstruction And Simulation Of Civil War Garments Recovered From The H. L. Hunley, Hanna George

All Theses

Costumes are a crucial element in the representation of historical figures. Beyond reflecting aesthetic trends, clothing is closely intertwined with culture, economics, and the personal history of the individual who wore them. Fabric simulation allows for the digital creation and animation of garments too fragile or incomplete to be used directly in film or education. This thesis demonstrates the digital reconstruction of the clothing worn by H. L. Hunley crew member James Wicks. The H. L. Hunley is a submarine from the Civil War. It was the first in history to successfully sink an enemy ship, but never returned from …

Teachers’ Views Of Situational Factors Influencing Teaching Efficacy During The Covid-19 Pandemic, Holly Gillespie

All Theses

COVID-19 created many disruptions in the field of education as teachers and administrators navigated the many changing protocols affecting learning environments and pedagogy over the past two years. This thesis examines secondary teachers’ perceptions of managing these protocols through their lived experiences while teaching during the COVID-19 pandemic through the 2020-2021 school years. Previous research has shown a teacher’s sense of self-efficacy is related to both learning situations and teacher performance. However, research exploring the impact COVID-19 has had on teachers and their learning environments is limited. This qualitative exploratory study fills the gap by investigating situational factors in learning …

Activation Of The Ppar Nuclear Receptors By Oxylipins And Their Potential Effects On Differentiation And Gene Expression In Myocytes, Jazmine Eccles

All Theses

Obesity and non-alcoholic fatty liver diseases are a growing issue worldwide. Decreased expression of CYP2B6 is associated with obesity in humans and Cyp2b-null mice are diet-induced obese; however fatty liver disease is worse in humanized CYP2B6 transgenic mice than WT or Cyp2b-null mice despite lower obesity. CYP2B6 produces several oxylipins with preference for metabolism at the 9- and 13-positions with 9-HODE and 13-HODE (from linoleic acid; n-6), and 9-HOTrE and 13-HOTrE (from a-linolenic acid; n-3) preferentially produced. Oxylipins serve as signaling molecules for many processes, such as inflammatory pathways, lipid distribution, and lipid metabolism. Due to their diversity, oxylipins can …

Crime Reduction And Reformation In South Carolina: An Analysis Of Sb 1154, Matthew Torres

All Theses

This thesis evaluates the policy response to the criminal justice crisis of South Carolina, Senate Bill (SB) 1154, dubbed the Omnibus Crime Reduction and Sentencing Reform Act of 2010. SB 1154 adopted a series of “common sense” reforms, including new charges constituting as violent crimes, redefined sentences in the form of reduced penalties and harsher penalties for nonviolent and violent crimes respectively, mandated supervision of individuals on probation or parole, and credit programs for early release of inmates among other provisions intended to reduce inmate population, recidivism and violent crime rates.

I examine three key factors: inmate, imprisonment and violent …

Bioleaching Of Arsenic From Agricultural Soils, Zachary Gilstrap

All Theses

Arsenic is a ubiquitous trace metalloid. However, increasing concentrations of arsenic in soil and water is a problem the world faces due to inputs from anthropogenic sources such as mining, milling, agriculture, and coal ash. One method of remediation for these arsenic-contaminated sites is bioleaching. While it is most often used to extract metals from raw ore, it is a viable and environmentally friendly method to remove the same metals from soil. Bioleaching removes metals by cultivating living organisms such as the fungi Aspergillus niger (A.niger) or the bacterium Thiobacillus ferrooxidans (T.ferrooxidans). This research aimed to investigate A. …

Moderate, Overlooked Weather Events And Local Labor Markets, Michael Ellis

All Theses

A substantial amount of research exists on the economic effects of natural disasters and extreme weather, as well as comparatively minor variations in daily weather conditions such as precipitation, temperature, and wind. I construct a sample of over a million storms that range from intense daily weather conditions to extreme weather. I analyze written descriptions of the damages caused by many of these storms to identify 93,743 weather events within the sample that appear to be underemphasized in the existing economic literature. These storms cause more damage and disruptions to commerce than 63.5% of storms in the sample, yet do …

A 3d Tissue Engineering Model To Study Mitral Valve Annulus Calcification Under Diabetic Conditions, Erin James

All Theses

The most complex heart valve is the mitral valve (MV). Many pathologies can affect the MV, including stenosis, regurgitation, prolapse, and mitral annulus calcification (MAC). MAC is chronic degeneration of the annulus, which is the fibrous, saddle-shaped “ring” that can contract and relax with the myocardium. The prevalence of MAC is around 15% but increases in patients with other cardiovascular diseases and risk factors. It is also thought to increase in patients with type 2 diabetes, but MAC has not been properly characterized within this population because of confounding factors such as cardiac disease and kidney disease. The goal of …

An Evaluation Of Energy Consumption Comparing Conventional Water Treatment Plants To Microfiltration And Ultrafiltration Water Treatment Plants, Danielle Larsen

All Theses

The use of low-pressure, hollow fiber microfiltration (MF) and ultrafiltration (UF) membranes has become increasingly prominent in drinking water treatment plant configurations over the past 30 years. At the same time, the topics of energy efficiency and sustainability in industry are becoming progressively more important. Although MF and UF are highly successful in filtration, they are generally thought of as significant energy consumers; however, the data have not yet been available to compare multiple plants in various contexts to determine the factors that most affect energy use. This research compares the energy use of MF and UF treatment plants to …

Development Of A New Affinity Membrane For Rapidly Purifying Non-Antibody Proteins, Friendship Edioma

All Theses

This thesis project describes the modification and evaluation of a new affinity membrane for rapid chromatographic purification of non-antibody proteins. The affinity membrane utilizes Im7/CL7 coupling technology developed by Dr. Vassylyev's lab at the University of Alabama Birmingham (UAB), licensed by TriAltus Bioscience. The behavior of the membrane was evaluated using purified CL7-tagged Cas9 as my model protein for static and dynamic binding capacity analysis.

Chapter one provides an overview on biopharmaceutical drug production process development. I discuss how protein drugs are produced, isolated, and purified from the cell supernatant after upstream phases are completed. Despite increasing demands for biologics, …

The Evacuation Simulation Of Wheelchair Users In A Building Fire: An Initial Dynamic Characterization Of Structural Egress Components, Haley Hostetter

All Theses

People with disabilities are one of the most vulnerable groups involved in building fires. According to the U.S. Fire Administration, in the United States alone, an estimated 700 home fires involve people with physical disabilities each year while over 1700 involve those with mental health disorders. Despite this, the current body of literature shows few studies focused on the evacuation of disabled people. This is a direct result of past and present social injustice on people with disabilities and has resulted in high injury and death rates during fires. To combat this, enrich the literature, and improve their experiences in …

The Effects Of Short Chain Fatty Acids On Clostridioides Difficile Growth, Sporulation, And Toxin Production, Michelle Baldassare

All Theses

Short chain fatty acids (SCFAs) are products of bacterial fermentation that help maintain important gut functions such as the intestinal barrier, signaling, and immune homeostasis. The main SCFAs acetate, propionate, and butyrate have demonstrated beneficial effects for the host, including importance in combatting infections caused by pathogens such as Clostridioides difficile. C. difficile infection (CDI) is a public health concern and causes 30,000 deaths each year mainly due to the overuse of antibiotics. Despite the potential role of SCFAs in mitigating C. difficile infection, their direct effect on C. difficile remains unclear. Through a set of in-vitro experiments, we …

Climbing The Masculine Hierarchy: Examining Constructions Of Masculinity Through Incel Identities, Alyssa Jewel Davis

All Theses

In a gender-segregated digital space known as the Manosphere, a group of men calling themselves “Incels,” or involuntary celibates, express feelings of hostility and hatred towards women. Incels hold a low position on a masculine hierarchy where the men who hold the most power in society are those who have access to women’s emotional and sexual services. Incels are characterized by feelings of entitlement to women’s services and aggrievement by their inability to access them. As a result, they often appear to fantasize about or engage in acts of violence to compensate for their lack of masculine privilege. However, there …

Yoga In Criminal Justice Facilities: A Descriptive Study, Rachel Broome

All Theses

The United States currently incarcerates more individuals than any other country in the world, creating an effect known as mass incarceration. While incarcerated, individuals’ physical and mental health can be affected. Yoga is a feasible, cost-effective modality that may be utilized in criminal justice facilities, to assist in the process of improving the physical and mental health of individuals who are incarcerated or in confinement. The purpose of this study was to explore the structure of yoga programs that are being implemented throughout United States criminal justice facilities and the effects that the COVID-19 pandemic had on programs. Through a …

A Comparative Study Of Physical Cinematography That Influences Layouts In Animated Production, Daniel C. Hudson

All Theses

What makes good cinematography? There are many examples of cinematogra- phy that stand out in the minds of viewers, yet many cant exactly say with certainty why these shots are considered great. Is it because they convey the visual and emo- tional content of their stories successfully, the technical brilliance of the frame, or both? Whether realistic or exaggerated, the range of cinematography tools and tech- niques are proof that creativity and an eye for detail are the most important elements in great cinematography. Cinematography includes composition, movement, lighting, and other visual information within the frame. This is true in …

Depot-Specific Patterns Of Mrna And Mirna Gene Expression In Adipose Tissue From Texel-Cross And Suffolk Lambs, Aliute-Nkoyo Udoka

All Theses

This work hypothesizes that miR-199a-3p influences SCD-1 expression and monounsaturated fat content of lamb fat depots. The objectives of this study were to: 1) measure miR-199a-3p, SCD1, and fatty acid composition in various tissues (longissimus muscle and liver) and fat depots (omental, mesenteric, subcutaneous, intermuscular, perirenal) in Suffolk and Texel-sired lambs, and 2) evaluate the role of miR-199a-3p in adipocyte hypertrophy in ovine stromal vascular (SV) cells in vitro. Suffolk lambs (n=4) and Texel-Suffolk cross lambs (n=5) were finished to day 203 of age. The liver, the longissimus muscle, kidney fat, mesenteric fat, omental fat, subcutaneous fat, and intermuscular fat …

The Influence Of A Professional Sport Team’S Philanthropic Initiative On Fans’ Corporate Social Responsibility (Csr) Participation Intention, Young Suk Oh

All Dissertations

Within corporate social responsibility (CSR) research, there has been significant evidence of the positive impacts that CSR could bring to businesses. However, few studies, especially in the context of sport, have focused on how CSR benefits an actual social cause. Therefore, through a three-article format, the overall purpose of the dissertation is to examine how a professional sport team could use its CSR as a social marketing strategy to increase its fans’ willingness to participate in the team’s CSR program in contributing to a specific social cause. Specifically, Article 1 assesses whether CSR participation intention significantly differs among fans with …

Characterization Of The Wee1 Homologues And The Investigation Of Factors Promoting Cellular Enlargement In Cryptococcus Neoformans, Rodney J. Colón Reyes

All Dissertations

Cryptococcus neoformans is an opportunistic fungal pathogen, infecting mainly immunocompromised individuals. As the main cause of cryptococcosis, it is responsible for over 180,000 deaths every year. As an environmental yeast, it has unique adaptations that allow it to proliferate in the human host. Among these adaptations its capacity to transition to an extreme phenotype known as Titan cells is of special interest to researchers. With sizes above 10 um and able to reach 70 um or more in cell size. This size is accompanied with a large vacuole, larger polysaccharide capsule, and an increased resistance to fluconazole (FLC). FLC is …

The Forgotten Faith: The Experiences Of Enslaved Muslims And The Influence Of Islam In The United States From 1730-1864, Amani Altwam

All Theses

Muslims were present in North America before the establishment of the

American/British colonies. The first Muslims in America were not citizens, but

enslaved Africans forced into the slave trade in the eighteenth century. Muslim slaves

in America were much more prevalent than anyone could have imagined and yet, the

religion of these slaves was rarely ever brought to the surface. In this thesis, I argue

that Muslim slaves not only existed in America but most of them were literate in

multiple languages, well-educated, and were capable of holding on to a set of beliefs.

History books and previous literature have …

Tissue-Engineered Living Pulmonary Valve For Young Adult Patients, Erica Hoskins

All Theses

Cardiovascular disease (CVD) is a non-communicable disease responsible for 659,000 deaths annually in the United States. While CVD can affect all components of the cardiovascular system, heart valve disease is responsible for 25,000 deaths yearly. Of specific interest is the pulmonary valve since there is limited research on resolving pathologies that affect it. Like the aortic and mitral valves, common pathologies include stenosis, regurgitation, and atresia. Notably, the pulmonary valve requires repair and replacement in pediatric patients and young adults due to congenital disabilities. Even with technological advances in valve replacements, limitations still present themselves for use with younger patients …

Shelf Life Of Aquaponically-Grown Finstar Lettuce In Different Oxygen Transmission Rate Films, Katherine White

All Theses

The effects of oxygen transmission rate of packaging material on the shelf life of aquaponically-grown Finstar lettuce was studied. Parameters of packaging headspace gas composition (oxygen and carbon dioxide concentrations) and lettuce pH, percentage weight loss, total aerobic microorganisms, and color were analyzed every ten days for sixty days. Finstar lettuce was stored at 4^○C in four different types of packages (treatments), including a clamshell package and three film bags with oxygen transmission rates (OTR) of 3.0-6.0 cc/(m²/24 hr/1 atm), 80-90 cc/(m²/24 hr/1 atm), and >225 cc/(m²/24 hr/1 atm). Lettuce stored in …

Digital And Gradient Refractive Index Planar Optics By Nanoimprinting Porous Silicon, Anna Hardison

All Theses

Due to the drawbacks of traditional refractive optics, the implementation of planar or nearly planar optical devices has been of research interest for over a century. Subwavelength gratings are a particularly promising option for creating flat optical devices; however, the implementation of subwavelength grating-based optics is limited by fabrication constraints. Recently, we implemented flat optical devices using the nanoimprinting of refractive index (NIRI) process, a process which was pioneered in a previous study but remained largely unproven in terms of device fabrication. The planar, gradient index microlenses we fabricated were found to possess an effective medium similar to a subwavelength …

Exploring Visitor Perceptions Of Popular Culture Tourism In Sleepy Hollow, New York, Danielle Baker Kreh

All Theses

Popular culture tourism can present itself in many forms including travel based on popular literature, film, and television series. Popular culture tourism destinations exist from the assumption that tourists wish to visit destinations based on their popular culture connections. In fact, destinations may choose to emphasize their popular culture connections for the purpose of attracting more visitors. Such is the case with Sleepy Hollow, New York, which has many popular culture connections including the original short story “The Legend of Sleepy Hollow” by Washington Irving. Using the village of Sleepy Hollow as an example, the purpose of this study is …

Opioid-Related Content On Twitter And The Impact Of Covid-19 Government Stimulus Distribution, Alyssa Seeman

All Theses

The United States has continuously faced an opioid epidemic that has resulted in a severe loss of human life. The coronavirus pandemic began in December 2019 and affected many aspects of daily life. One result of this pandemic was government financial aid in the form of stimulus checks that were directly deposited into peoples’ bank accounts. This study aims to understand better the impact stimulus checks had on opioid overdose rates within America by using content collected from Twitter to gauge public opinion. The sample consisted of a stratified random sample of 600 overall tweets that contained at least one …

Generating Sex Trafficking Networks From Text Documents, Maria Diaz

All Theses

Qualitative coding is a long and strenuous process that requires a well-skilled investigator. Natural language processing techniques have made leaps and bounds as far as usability and application domain, although it does not work for every task. In this work, we have created a natural language processing framework to help qualitative coders automatically obtain the nodes and node arcs from federal case files, dockets, and indictments within a sex trafficking network. The produced nodes and arcs allows us to perform network modeling by providing us with the information needed to create network structures that can then be used for interdiction …