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Biochemical Study Of Endonuclease V And Its Application In Mutation Scanning, Honghai Gao May 2007

Biochemical Study Of Endonuclease V And Its Application In Mutation Scanning, Honghai Gao

All Dissertations

The integrity of the genetic information encoded by DNA is essential to all living organisms, yet the reactive bases of DNA are constantly attacked by endogenous and exogenous agents resulting in as many as one million individual molecular lesions per cell per day. Excessive DNA damage or deficiency in DNA repair enzymes may cause cancer, premature aging, and neurodegenerative diseases.
Endonuclease V (Endo V) is a DNA repair enzyme which can recognize all four types of DNA deamination products, specifically, uracil, hypoxanthine, xanthine and oxanine. It was also shown that endo V can recognize mismatches. We screened about 60 mutants …


Extraction Method Development And In Vivo And In Vitro Toxicity Studies Of The Etiologic Agent Of Avian Vacuolar Myelinopathy, Faith Wiley May 2007

Extraction Method Development And In Vivo And In Vitro Toxicity Studies Of The Etiologic Agent Of Avian Vacuolar Myelinopathy, Faith Wiley

All Dissertations

Avian vacuolar myelinopathy (AVM) is a neurological disease affecting birds in the southeastern United States. The cause of the disease has not yet been determined but is believed to be a naturally produced toxin and is associated with aquatic vegetation. Current research on AVM is limited to in vivo studies utilizing whole tissue or vegetative samples. The objectives of this research were to develop extraction methods for isolating the putative AVM toxin from vegetative samples and to develop an in vitro bioassay for detection and study of the toxin. Samples of vegetation were collected from reservoirs known to be affected …


Three Essays In Political Economy, Melissa Yeoh May 2007

Three Essays In Political Economy, Melissa Yeoh

All Dissertations

The first essay provides a theoretical framework that informs the ongoing debate regarding the effect of conservation easement subsidies on local property tax revenue. Supporters claim that conservation easements increase property tax revenue by providing environmental amenities that increase the value of adjacent properties. Critics argue that they decrease property tax revenue by lowering land values and shrinking the tax base. In instances when local property tax revenue decline due to the income tax deductibility of conservation easements, the decline is larger when the demand for land is more elastic, the proportion of non-agricultural land in the county is larger, …


Physiochemical Properties And Bioactivities Of Tea Seed (Camellia Oleifera) Oil, Yen-Hui Chen May 2007

Physiochemical Properties And Bioactivities Of Tea Seed (Camellia Oleifera) Oil, Yen-Hui Chen

All Theses

Tea seed (Camellia oleifera) oil has been used for cooking in China and other Asian countries for more than a thousand years. This study determined the fatty acid composition and tocopherol content through chromatography-flame ionization (GC-FID) and high performance liquid chromatography-UV (HPLC-UV) , respectively. The results showed that the tea seed oil (TSE) was mainly composed of 18.30% palmitic acid (C16:0), 54.95% oleic acid (C18:1), 22.41% linoleic acid (C18:2), and 0.17% linolenic acid (C18:3). Its α-tocopherol content was found the 21.13 mg/100 g oil. Moreover, the antioxidant capacities of methanolic extract of TSE and the remaining meal (Meal)were …


The Tiger Vol. 101 Issue 11 2007-04-20, Clemson University Apr 2007

The Tiger Vol. 101 Issue 11 2007-04-20, Clemson University

Tiger Newspapers

No abstract provided.


The Tiger Vol. 101 Issue 9 2007-04-06, Clemson University Apr 2007

The Tiger Vol. 101 Issue 9 2007-04-06, Clemson University

Tiger Newspapers

No abstract provided.


Outdoor Recreation Use Survey Of South Carolina's Jocassee Gorges, Thomas Warren Apr 2007

Outdoor Recreation Use Survey Of South Carolina's Jocassee Gorges, Thomas Warren

All Theses

The Jocassee Gorges Natural Area is approximately 43,500 acres in size and is managed as a Wildlife Management Area by the South Carolina Department of Natural Resources (SCDNR). The purpose of the Jocassee Gorges Outdoor Recreation Use Survey was to determine the outdoor recreation activities, participation rates, and resource uses of the Jocassee Gorges. Secondary objectives included documenting and describing the current use of on-site visitors, participation rates, and temporal and spatial distribution patterns of use. The current and past use by local residents of the Jocassee Gorges were documented. A total of 263 on-site visitors were contacted, of which …


Associating Larvae And Adults Of Chinese Hydropsychidae Caddiflies (Insecta:Trichoptera) Using Dna Sequences, Xin Zhou, Karl M. Kjer, John C. Morse Apr 2007

Associating Larvae And Adults Of Chinese Hydropsychidae Caddiflies (Insecta:Trichoptera) Using Dna Sequences, Xin Zhou, Karl M. Kjer, John C. Morse

Publications

The utility of hydropsychid (Trichoptera:Hydropsychidae) caddisfly larvae for freshwater biomonitoring has been demonstrated, but the major impediment to its implementation has been the lack of species-level larval descriptions and illustrations. A rapid and reliable molecular protocol that also uses morphology is proposed because conventional approaches to associating undescribed larvae with adults have been slow and problematic. Male adults were identified before DNA sequence analyses were used. These identifications established morphospecies boundaries that were mapped on phylograms constructed from 2 independent gene fragments: mitochondrial cytochrome c oxidase subunit I (COI) and large subunit (28S) nuclear ribosomal DNA expansion fragment D2 (D2). …


The Tiger Vol. 101 Issue 8 2007-03-30, Clemson University Mar 2007

The Tiger Vol. 101 Issue 8 2007-03-30, Clemson University

Tiger Newspapers

No abstract provided.


The Tiger Vol. 101 Issue 4 2007-02-16, Clemson University Feb 2007

The Tiger Vol. 101 Issue 4 2007-02-16, Clemson University

Tiger Newspapers

No abstract provided.


The Tiger Vol. 101 Issue 3 2007-02-09, Clemson University Feb 2007

The Tiger Vol. 101 Issue 3 2007-02-09, Clemson University

Tiger Newspapers

No abstract provided.


The Tiger Vol. 101 Issue 2 2007-02-02, Clemson University Feb 2007

The Tiger Vol. 101 Issue 2 2007-02-02, Clemson University

Tiger Newspapers

No abstract provided.


Urban Physiology: City Ants Possess High Heat Tolerance, Michael W. Sears, Michael J. Angilletta, Robbie S. Wilson, Amanda C. Niehaus, Pedro L. Ribeiro, Carlos A. Navas Feb 2007

Urban Physiology: City Ants Possess High Heat Tolerance, Michael W. Sears, Michael J. Angilletta, Robbie S. Wilson, Amanda C. Niehaus, Pedro L. Ribeiro, Carlos A. Navas

Publications

Urbanization has caused regional increases in temperature that exceed those measured on a global scale, leading to urban heat islands as much as 12°C hotter than their surroundings. Optimality models predict ectotherms in urban areas should tolerate heat better and cold worse than ectotherms in rural areas. We tested these predications by measuring heat and cold tolerances of leaf-cutter ants from South America’s largest city (São Paulo, Brazil). Specifically, we compared thermal tolerances of ants from inside and outside of the city. Knock-down resistance and chill-coma recovery were used as indicators of heat and cold tolerances, respectively. Ants from within …


The Tiger Vol. 101 Issue 1 2007-01-26, Clemson University Jan 2007

The Tiger Vol. 101 Issue 1 2007-01-26, Clemson University

Tiger Newspapers

No abstract provided.


Comparative Study Of Haptic And Visual Feedback For Kinesthetic Training Tasks, Ravikiran Singapogu Jan 2007

Comparative Study Of Haptic And Visual Feedback For Kinesthetic Training Tasks, Ravikiran Singapogu

All Theses

Haptics is the sense of simulating and applying the sense of human touch. Application of touch sensations is done with haptic interface devices. The past few years has seen the development of several haptic interface devices with a wide variety of technologies used in their design. This thesis introduces haptic technologies and includes a survey of haptic interface devices and technologies. An improvement in simulating and applying touch sensation when using the Quanser Haptic Wand with proSense is suggested in this work using a novel five degree-of-freedom algorithm. This approach uses two additional torques to enhance the three degree-of-freedom of …


Comprehensive Annual Financial Report For The Year Ended June 30, 2006, Clemson University Jan 2007

Comprehensive Annual Financial Report For The Year Ended June 30, 2006, Clemson University

Annual Comprehensive Financial Report

No abstract provided.


Supplemental Schedules For The Year Ended June 30, 2006, Clemson University Jan 2007

Supplemental Schedules For The Year Ended June 30, 2006, Clemson University

Annual Comprehensive Financial Report

No abstract provided.


Supplemental Schedules For The Year Ended June 30, 2007, Clemson University Controller's Office Jan 2007

Supplemental Schedules For The Year Ended June 30, 2007, Clemson University Controller's Office

Supplemental Schedules

No abstract provided.


Copyright Crash Course - Who Owns What?, Georgia K. Harper Jan 2007

Copyright Crash Course - Who Owns What?, Georgia K. Harper

A repository of copyright educational resources for higher education

Copyright Crash Course's page explaining ownership of materials, how to obtain copyright and how to protect your own intellectual property through copyright.


The Tiger Orientation Issue 2007, Clemson University Jan 2007

The Tiger Orientation Issue 2007, Clemson University

Tiger Newspapers

No abstract provided.


Moistened Seeds Increase Rodent Trap Success, Michael W. Sears, John C. Tull Jan 2007

Moistened Seeds Increase Rodent Trap Success, Michael W. Sears, John C. Tull

Publications

Seed moisture has been shown to influence the rates of seed cache removal by rodents. Although the precise mechanism is not known, this knowledge might prove useful in field applications. We examined whether moistened bait would increase trap success in desert rodent populations. We placed traps 15 m apart in grids within a 500-ha study area and randomly baited traps with either dry or moistened seeds. We found that traps baited with moistened seeds had 34.9% higher success than traps baited with dry seeds (n = 190, Χ2 = 5.389, df = 1, P = 0.020). Our results …


Wind Damage And Salinity Effects Of Hurricanes Katrina And Rita On Coastal Baldcypress Forests Of Louisiana, William Conner, Thomas W. Doyle, Richard H. Day, Ken W. Krauss, Christopher M. Swarzenski Jan 2007

Wind Damage And Salinity Effects Of Hurricanes Katrina And Rita On Coastal Baldcypress Forests Of Louisiana, William Conner, Thomas W. Doyle, Richard H. Day, Ken W. Krauss, Christopher M. Swarzenski

Publications

The frequency of hurricane landfall in a given coastal stretch may play a more important role in the ecology of coastal forests than previously thought because of direct and indirect impacts of fallen trees and the introduction of salt water that lingers long after the storm passes. Findings show that surge events can inundate interior freshwater forests many miles from the coast and elevate soil salinities twofold to threefold. These elevated salinities may contribute to delayed mortality of certain tree species and set the stage for eventual forest decline and dieback.


Science And The Storms: The Usgs Response To The Hurricanes Of 2005 - Chapter Six: Ecological Impacts, William Conner, Stephen Faulkner, Wylie Barrow, Brady Couvillion, Lori Randall, Michael Baldwin Jan 2007

Science And The Storms: The Usgs Response To The Hurricanes Of 2005 - Chapter Six: Ecological Impacts, William Conner, Stephen Faulkner, Wylie Barrow, Brady Couvillion, Lori Randall, Michael Baldwin

Publications

Ecological impacts from the hurricanes of 2005 affected both vegetation and the animals that depend on coastal habits on land and in water. Discussed in this section are migratory birds, coastal marsh vegetation, chenier forests, coastal floodplain forests, mangrove forests, estuaries, and the endangered manatee.


The Tiger Career Fair Fall 2007, Clemson University Jan 2007

The Tiger Career Fair Fall 2007, Clemson University

Tiger Newspapers

No abstract provided.


Are Some Environmentalists More Equal Than Others?, Circa 2007, Clinton H. Whitehurst Jr. Jan 2007

Are Some Environmentalists More Equal Than Others?, Circa 2007, Clinton H. Whitehurst Jr.

Publications

No abstract provided.


Faculty Manual, 2007-2008, Clemson University Jan 2007

Faculty Manual, 2007-2008, Clemson University

Faculty Manuals

No abstract provided.


Clemson Graduate School Catalog, 2007-2008, Clemson University Jan 2007

Clemson Graduate School Catalog, 2007-2008, Clemson University

Clemson Graduate Announcements

No abstract provided.


Elastic Degradable Polyurethanes For Biomedical Applications, Changhong Zhang Dec 2006

Elastic Degradable Polyurethanes For Biomedical Applications, Changhong Zhang

All Theses

Several series of polyurethanes were synthesized with linear or crosslinked structures by using different synthesis routes. Two studies are mentioned: (1) the synthesis of degradable polyurethanes with linear structure and the investigation of the elasticity and cytophilicity of the materials as function of the chain extender, and (2) the synthesis and the investigation of the biocompatibility, degradation, hydrophilicity and mechanical properties of the polyurethane-based hydrogels with crosslinked structure.


Optimizing Production Methods For Artifical Silk Proteins Through Bioreactor And Purification Studies Of Recombinant Proteins Expressed From Pichia Pastoris, Aaron Ramey Dec 2006

Optimizing Production Methods For Artifical Silk Proteins Through Bioreactor And Purification Studies Of Recombinant Proteins Expressed From Pichia Pastoris, Aaron Ramey

All Theses

Advancements in the field of biomaterials are being made to produce an artificial silk fiber. A gene was constructed which utilized components from both the dragline silk of Nephila clavipes and nematode collagen. For this engineered protein, the yeast strain Pichia pastoris was chosen to be the host organism. Previous research has shown P. pastoris to have comparatively low amounts of specific protein productivity. Therefore, this problem must be compensated for by obtaining extremely high cell densities. The main focus of this study was to optimize the fermentation parameters of transgenic yeast cultures within a bioreactor in order to increase …


The Effects Of Suspended Sediment On The Aquatic Organisms Daphnia Magna And Pimephales Promelas, Neil Capper Dec 2006

The Effects Of Suspended Sediment On The Aquatic Organisms Daphnia Magna And Pimephales Promelas, Neil Capper

All Theses

Land use change results in significant amounts of soil migrating into aquatic streams during storm events. This suspended sediment, even in the absence of adsorbed contaminants, may be a significant stressor to aquatic organisms. The data on ecological effects of suspended sediment has mainly focused on salmonids or ecosystem level consequences such as habitat alteration. Few studies have quantified physiologic effects of suspended solids on water column organisms. The purpose of this research was to determine the effects of suspended clay on Daphnia magna and Pimephales promelas. Results of 7 d toxicity tests with D. magna in natural and …