Digital Commons Network^™

Multilocus Coalescent Analyses Reveal The Demographic History Of Mouse Lemur Sister Species., Christopher Blair, Kellie L. Heckman, Amy L. Russell, Anne D. Yoder

Publications and Research

Debate continues as to whether allopatric speciation or peripatric speciation through a founder effect is the predominant force driving evolution in vertebrates. The mouse lemurs of Madagascar are a system in which evolution has generated a large number of species over a relatively recent time frame. Here, we examine speciation patterns in a pair of sister species of mouse lemur, Microcebus murinus and M. griseorufus. These two species have ranges that are disparately proportioned in size, with M. murinus showing a much more extensive range that marginally overlaps that of M. griseorufus. Given that these two species are …

Prenatal Cocaine Exposure Uncouples Mglur1 From Homer1 And Gq Proteins, Kalindi Bakshi, Raminder Parihar, Satindra K. Goswami, Melissa Walsh, Eitan Friedman, Hoau-Yan Wang

Publications and Research

Cocaine exposure during gestation causes protracted neurobehavioral changes consistent with a compromised glutamatergic system. Although cocaine profoundly disrupts glutamatergic neurotransmission and in utero cocaine exposure negatively affects metabotropic glutamate receptor-type 1 (mGluR1) activity, the effect of prenatal cocaine exposure on mGluR1 signaling and the underlying mechanism responsible for the prenatal cocaine effect remain elusive. Using brains of the 21-day-old (P21) prenatal cocaine-exposed rats, we show that prenatal cocaine exposure uncouples mGluR1s from their associated synaptic anchoring protein, Homer1 and signal transducer, Gq/11 proteins leading to markedly reduced mGluR1-mediated phosphoinositide hydrolysis in frontal cortex (FCX) and hippocampus. This prenatal cocaine-induced effect …

Altering Murine Leukemia Virus Integration Through Disruption Of The Integrase And Bet Protein Family Interaction, Sriram Aiyer, G.V.T. Swapna, Nirav Malani, James M. Aramini, William M. Schneider, Matthew R. Plumb, Mustafa Ghanem, Ross C. Larue, Amit Sharma, Barbara Studamire, Mamuka Kvaratskhelia, Frederic D. Bushman, Gaetano T. Montelione, Monica J. Roth

Publications and Research

We report alterations to the murine leukemia virus (MLV) integrase (IN) protein that successfully result in decreasing its integration frequency at transcription start sites and CpG islands, thereby reducing the potential for insertional activation. The host bromo and extraterminal (BET) proteins Brd2, 3 and 4 interact with the MLV IN protein primarily through the BET protein ET domain. Using solution NMR, protein interaction studies, and next generation sequencing, we showthat the C-terminal tail peptide region ofMLV IN is important for the interaction with BET proteins and that disruption of this interaction through truncation mutations affects the global targeting profile of …

Chronic Corticosterone Exposure Persistently Elevates The Expression Of Memory-Related Genes In The Lateral Amygdala And Enhances The Consolidation Of A Pavlovian Fear Memory, Melissa S. Monsey, Lara M. Boyle, Melinda L. Zhang, Caroline P. Nguyen, Hope G. Kronman, Kristie T. Ota, Ronald S. Duman, Jane R. Taylor, Glenn E. Schafe

Publications and Research

Chronic exposure to stress has been widely implicated in the development of anxiety disorders, yet relatively little is known about the long-term effects of chronic stress on amygdala-dependent memory formation. Here, we examined the effects of a history of chronic exposure to the stress-associated adrenal steroid corticosterone (CORT) on the consolidation of a fear memory and the expression of memory-related immediate early genes (IEGs) in the lateral nucleus of the amygdala (LA). Rats received chronic exposure to CORT (50 μg/ml) in their drinking water for 2 weeks and were then titrated off the CORT for an additional 6 days followed …

Chagas Disease In The 21st Century: A Public Health Success Or An Emerging Threat?, Kevin M. Bonney

Publications and Research

Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, is a major public health burden in Latin America and a potentially serious emerging threat to a number of countries throughout the world. Although public health programs have significantly reduced the prevalence of Chagas disease in Latin America in recent decades, the number of infections in the United States and non-endemic countries in Europe and the Western Pacific Region continues to rise. Moreover, there is still no vaccine or highly effective cure available for the approximately 10 million people currently infected with T. cruzi, a third of which will develop potentially …

An Optogenetic Gene Expression System With Rapid Activation And Deactivation Kinetics, Laura B. Motta-Mena, Anna Reade, Michael J. Mallory, Spencer Glantz, Orion D. Weiner, Kristen W. Lynch, Kevin H. Gardner

Advanced Science Research Center

Optogenetic gene expression systems can control transcription with spatial and temporal detail unequaled with traditional inducible promoter systems. However, current eukaryotic light-gated transcription systems are limited by toxicity, dynamic range, or slow activation/deactivation. Here we present an optogenetic gene expression system that addresses these shortcomings and demonstrate its broad utility. Our approach utilizes an engineered version of EL222, a bacterial Light-Oxygen-Voltage (LOV) protein that binds DNA when illuminated with blue light. The system has a large (>100-fold) dynamic range of protein expression, rapid activation (< 10 s) and deactivation kinetics (< 50 s), and a highly linear response to light. With this system, we achieve light-gated transcription in several mammalian cell lines and intact zebrafish embryos with minimal basal gene activation and toxicity. Our approach provides a powerful new tool for optogenetic control of gene expression in space and time.

Vimentin And Notch As Biomarkers For Breast Cancer Progression, Gloria M. Calaf, Adayabalam S. Balajee, Maria T. Montalvo-Villagra, Mariana Leon, Navarrete M. Daniela, Raúl González Alvarez, Debasish Roy, Gopeshwar Narayan, Jorge Abarca-Quinones

Publications and Research

Breast cancer, the most common spontaneous malignancy diagnosed in women, is a classical model of hormone dependency as it is associated with prolonged exposure to female hormones. Different cytoplasmic proteins are important in the transformation of a normal cell to an invasive tumor cell, and these include vimentin and Notch. To investigate the importance of these two genes and proteins in breast carcinogenesis, we used an in vitro breast cancer model system, in which an immortalized human breast epithelial cell line, MCF-10F, was malignantly transformed by exposure to low doses of high linear energy transfer α particle (150 keV/μm) radiation …

Advocate, March 2014, Vol. [25], No. [4], Advocate

The Advocate

TABLE OF CONTENTS:

From the Editor’s Desk: An Open Letter to the GC Community, The Advocate Editorial Staff (p. 3)

CUNY News in Brief:

- Albany Kills Dream, Shafts Libraries (p. 6)

- Gay Activists Protest St. Patrick’s Day Parade Ban, Camilo Gomez (p. 10)

Guest Columnist: U.S. Sailors and Marines Allege Fukushima Radiation Sickness, Amy Goodman (p. 12)

Women in the U.S. Workforce: Much to Celebrate, Much to Do, Cristina Pérez Díaz (p. 14)

CUNY Gives ROTC the Boot, Conor Tomás Reed (p. 16)

Copyright and Democracy: The Case for Protecting Intellectual Property in the Digital Age, Eric Bayruns …

Discovering Jewish Studies Collections In Academic Libraries: A Practical Guide, Izabella Taler

Publications and Research

The U.S. colleges and universities offering non-sectarian educational programs in Jewish Studies rely on the support of their academic libraries for research materials and library services. For college libraries which use Library of Congress Classification scheme, it is a common practice to integrate "studies" resources into their general library collections. Since Jewish Studies sources span a vast number of subjects within all major disciplines, shelving integration leads to the dispersion of all relevant sources and such dispersion in turn leads to a variety of problems for library professionals and library users. For collection development librarians the problems range from lack …

Large Cities Are Less Green, Erneson A. Oliveira, José S. Andrade, Hernán A. Makse

Publications and Research

We study how urban quality evolves as a result of carbon dioxide emissions as urban agglomerations grow. We employ a bottom-up approach combining two unprecedented microscopic data on population and carbon dioxide emissions in the continental US. We first aggregate settlements that are close to each other into cities using the City Clustering Algorithm (CCA) defining cities beyond the administrative boundaries. Then, we use data on CO2 emissions at a fine geographic scale to determine the total emissions of each city. We find a superlinear scaling behavior, expressed by a power-law, between CO2 emissions and city population with average allometric …

Vasovagal Oscillations And Vasovagal Responses Produced By The Vestibulo-Sympathetic Reflex In The Rat, Sergei B. Yakushin, Giorgio P. Martinelli, Theodore Raphan, Yongqing Xiang, Gay R. Holstein, Bernard Cohen

Publications and Research

Sinusoidal galvanic vestibular stimulation (sGVS) induces oscillations in blood pressure (BP) and heart rate (HR), i.e., vasovagal oscillations, as well as transient decreases in BP and HR, i.e., vasovagal responses, in isoflurane-anesthetized rats. We determined the characteristics of the vasovagal oscillations, assessed their role in the generation of vasovagal responses, and determined whether they could be induced by monaural as well as by binaural sGVS and by oscillation in pitch. Wavelet analyses were used to determine the power distributions of the waveforms. Monaural and binaural sGVS and pitch generated vasovagal oscillations at the frequency and at twice the frequency of …

C. Elegans Cep-1/P53 And Bec-1 Are Involved In Dna Repair, Sandy Hoffman, Daniel Martin, Alicia Meléndez, Jill Bargonetti

Publications and Research

p53 is a transcription factor that regulates the response to cellular stress. Mammalian p53 functions as a tumor suppressor. The C. elegans p53, cep-1, regulates DNA-damage induced germline cell death by activating the transcription of egl-1 and ced-13. We used the C. elegans model to investigate how, in the whole animal, different forms of DNA damage can induce p53-dependent versus p53-independent cell death and DNA repair. DNA damage was induced by ultraviolet type C (UVC) radiation, or 10-decarbamoyl mitomycin C (DMC, an agent known to induce mammalian p53-independent cell death). Wild-type or cep-1 loss-of-function mutant animals were assayed for germline …

Disassembly Of The Cystovirus Φ6 Envelope By Montmorillonite Clay, Karin A. Block, Adrianna Trusiak, Al Katz, Paul Gottlieb, Alexandra Alimova, Hui Wei, Jorge Morales, William J. Rice, Jeffrey C. Steiner

Publications and Research

Prior studies of clay–virus interactions have focused on the stability and infectivity of nonenveloped viruses, yielding contradictory results. We hypothesize that the surface charge distribution of the clay and virus envelope dictates how the components react and affect aggregation, viral stability, and infectivity. The bacteriophage Cystoviridae species φ6 used in this study is a good model for enveloped pathogens. The interaction between φ6 and montmorillonite (MMT) clay (the primary component of bentonite) is explored by transmission electron microscopy. The analyses show that MMT–φ6 mixtures undergo heteroaggregation, forming structures in which virtually all the virions are either sequestered between MMT platelet …

Strategies To Reduce The Harmful Effects Of Extreme Heat Events: A Four-City Study, Jalonne L. White-Newsome, Sabrina Mccormick, Natalie Sampson, Miatta A. Buxton, Marie S. O’Neill, Carina J. Gronlund, Linda Catalano, Kathryn C. Conlon, Edith A. Parker

Publications and Research

Extreme heat events (EHEs) are becoming more intense, more frequent and longer lasting in the 21st century. These events can disproportionately impact the health of low-income, minority, and urban populations. To better understand heat-related intervention strategies used by four U.S. cities, we conducted 73 semi-structured interviews with government and non-governmental organization leaders representing public health, general social services, emergency management, meteorology, and the environmental planning sectors in Detroit, MI; New York City, NY; Philadelphia, PA and Phoenix, AZ—cities selected for their diverse demographics, climates, and climate adaptation strategies. We identified activities these leaders used to reduce the harmful effects of …

The Evolutionary Function Of Conscious Information Processing Is Revealed By Its Task-Dependency In The Olfactory System, Andreas Keller

Publications and Research

Although many responses to odorous stimuli are mediated without olfactory information being consciously processed, some olfactory behaviors require conscious information processing. I will here contrast situations in which olfactory information is processed consciously to situations in which it is processed non-consciously. This contrastive analysis reveals that conscious information processing is required when an organism is faced with tasks in which there are many behavioral options available. I therefore propose that it is the evolutionary function of conscious information processing to guide behaviors in situations in which the organism has to choose between many possible responses.

Working Women: Contemporary Cinematic Costumes In "Desk Set" And "Working Girl", Karen Hummel Kinsley

Dissertations, Theses, and Capstone Projects

The contemporary cinematic costume designer is a visual storyteller. Through the dressing, or undressing of the actor's body, the costume designer narrates the cinematic character's physical and psychological journey from the first frame to the final credits. The costume designer creates the character's image by selecting specific garments and accessories that will inform the audience about the character's relationship with his cinematic environment.

The focus of this thesis is to examine how the contemporary cinematic costume designer unifies contemporary clothing with the actor's body to narrate a cinematic character's story. In order to understand how the costume designer creates a …

The Silent Guerrillas: Under Slavery, In Peasant Politics, And Throughout The Industrial And Social Factories, Kevin Van Meter

Dissertations, Theses, and Capstone Projects

In the work that follows, I will examine what political theorist James C. Scott calls "the hidden transcripts" of social struggle, which are written between the lines of the political record through the quotidian and clandestine practices of resistance and mutual aid that are the first and last recourse of subjugated populations. By engaging with literature on contemporary and historical social movements, I will explore the emergence and brief legibility of these practices among chattel slaves in the American South, the peasantry in various settings, and in a number of other industrial and social settings in an attempt to determine …

Exquisite Corpses: Fantasies Of Necrophilia In Early Modern English Drama, Linda K. Neiberg

Dissertations, Theses, and Capstone Projects

My dissertation examines representations of necrophilia in Elizabethan and Jacobean drama. From the 1580s, when London’s theatres began to flourish, until their closure by Parliament in 1642, necrophilia was deployed as a dramatic device in a remarkable number of plays. Exquisite Corpses analyzes the relationship between the English Reformation’s abolition of the doctrine of Purgatory and obsequies for the dead, and the frequent, often eroticized representations of dead bodies in the commercial theatre. Despite Protestant iconoclasm, “the cult of the cadaver,” as Eamon Duffy refers to it, was not readily relinquished and remained indelible in the cultural imagination. My project …

Data-Driven Synthesis Of Animations Of Spatially Inflected American Sign Language Verbs Using Human Data, Pengfei Lu

Dissertations, Theses, and Capstone Projects

Techniques for producing realistic and understandable animations of American Sign Language (ASL) have accessibility benefits for signers with lower levels of written language literacy. Previous research in sign language animation didn’t address the specific linguistic issue of space use and verb inflection, due to a lack of sufficiently detailed and linguistically annotated ASL corpora, which is necessary for modern data-driven approaches. In this dissertation, a high-quality ASL motion capture corpus with ASL-specific linguistic structures is collected, annotated, and evaluated using carefully designed protocols and well-calibrated motion capture equipment. In addition, ASL animations are modeled, synthesized, and evaluated based on samples …

Backward C Inside A Circle: Free Culture In Zines, Alycia Sellie

Dissertations, Theses, and Capstone Projects

Although zines made today utilize many forms of antiquated technologies such as the typewriter and the photocopier in their construction, they are a part of contemporary tinkering with intellectual property. This thesis examines free culture as it has been expressed in self-published zines made in the last thirty-five years. It looks at the licenses found in zines as conversations between a zine maker and a zine reader. Beyond just the legal implications, the cultural and ethical effects of licensing a zine are explored.

The internet is not the only place where people have played with intellectual property and toyed with …

Inter-Religious Relationships And Anxiety In The Regulation Of Automatic Inter-Religious Prejudice, Karla J. Felix

Dissertations, Theses, and Capstone Projects

Shared reality theory predicts and evidence suggests that inter-religious relationships are motivated to maintain or regulate interpersonal interactions with others. However, this motivation has been given little attention within the automatic attitude literature. This research is centered on the idea that automatic prejudice is moderated by two fundamental themes, shared reality and anxiety. These themes are reviewed to determine the degree to which participants socially tune to ingroup versus outgroup religious experimenters. In Experiment 1, automatic inter-religious attitudes toward Christian and Jewish experimenters were assessed via a subliminal prime procedure. Religious orientation (extrinsic, intrinsic) and regulation of inter-religious relationships were …

Phylogenetic Trees And Their Analysis, Eric Ford

Dissertations, Theses, and Capstone Projects

Determining the best possible evolutionary history, the lowest-cost phylogenetic tree, to fit a given set of taxa and character sequences using maximum parsimony is an active area of research due to its underlying importance in understanding biological processes. As several steps in this process are NP-Hard when using popular, biologically-motivated optimality criteria, significant amounts of resources are dedicated to both both heuristics and to making exact methods more computationally tractable. We examine both phylogenetic data and the structure of the search space in order to suggest methods to reduce the number of possible trees that must be examined to find …

Suburban Heat Islands: The Influence Of Residential Minimum Lot Size Zoning On Surface Heat Islands In Somerset County, New Jersey, Jennifer Renee Cox

Dissertations, Theses, and Capstone Projects

The process of suburbanization blurs regional bounds, forms mega-regions and fosters the expansion of multifaceted environmental problems, such as the urban heat island (UHI) effect. Defined by differences in air- and surface- temperature between rural and urban areas, UHI is the result of the characteristics of urbanization which modify the land surface condition, urban geometry, thermal properties of construction materials, anthropogenic heat and air pollution, which increase storage and re-radiation of heat to the atmosphere. Climate change is predicted to worsen the UHI effect. Hence, the objective of this research to characterize the UHI effect as it pertains to suburban …

Migration Plasticity As An Adaptation To Climate Change: The Spatial Distribution And Abundance Of A Subset Of Neotropical Migrant Landbirds Wintering In The Northeastern United States, Juliette Goulet

Dissertations, Theses, and Capstone Projects

There is a need for accurate predictions of the effects of climate change on wildlife populations. Bioclimatic relationships however are potentially complicated by various environmental factors operating at multiple spatial and temporal scales. Therefore, in order to test the hypothesis that climate constraints of winter bird distributions are modified by species-specific responses to weather and climate, I relied on Christmas Bird Count data (CBC). With nearly 100 years of data, the CBC is a valuable source of information on historic and recent changes in the status and distribution of birds during the early winter period in the United States and …

Testing Assumptions Of Coevolution In An Egg-Rejecting Brood Parasite Host: Uncovering Sensory, Cognitive, And Evolutionary Drivers Of Responses To Parasitism In American Robins (Turdus Migratorius), Rebecca Croston

Dissertations, Theses, and Capstone Projects

Hosts of brood parasitic birds face fitness costs associated with rearing unrelated offspring. In response, the recognition and rejection of parasitic eggs is a common host defense. Brown-headed cowbirds (Molothrus ater) challenge coevolutionary theory, because although they exploit over 200 host species, they lay non-mimetic eggs, and most hosts do not combat cowbird parasitism with egg rejection. American robins (Turdus migratorius) are one of a handful of cowbird hosts known to recognize and remove cowbird eggs from the nest. I addressed the mechanistic and evolutionary drivers of egg rejection in this host species, by disentangling the roles of spectral tuning …

The Characterization Of Pb2+ Toxicity In Rat Neural Development: An Assessment Of Pb2+ Effects On The Gaba Shift In Neural Networks And Implications For Learning And Memory Disruption, Lorenz Simon Neuwirth

Dissertations, Theses, and Capstone Projects

The toxic effects of Pb2+ on the developing rat nervous system has been investigated to assess early developmental GABAergic disruption and its implications with altering inhibitory learning and memory. This goal was achieved using a multi-systems approach: blood lead levels (clinical physiology), qRT-PCR (molecular genetics), brain and primary neuronal culture immunology (immunohistochemical and cellular approaches), physiological cellular components (synaptosomes and protein expression) and finally through learning and memory assessment with GABA mimetic drug manipulations in the intact animal (behavioral pharmacology). The influence of a 956ppm Pb2+ gestational diet (i.e. from birth to sacrifice) resulted in pup mean blood lead levels …

A Structural Approach To The Analysis Of Rock Music, Drew F. Nobile

Dissertations, Theses, and Capstone Projects

This dissertation situates itself in the middle of an ongoing debate about the applicability of Schenkerian analytical techniques to the analysis of pop and rock music. In particular, it investigates ways in which the standard conceptions of voice leading, harmonic function, and counterpoint may be updated to better apply to this new repertoire. A central claim is that voice-leading structure is intimately related to formal structure such that the two domains are mutually informing.

Part I of the dissertation focuses on harmonic and melodic theory. Chapter 2, "Harmonic Syntax," advocates for a conception of harmonic function based on syntax and …

The Sex Of The Cell Dictates Its Response, Carlos Ganesh Penaloza

Dissertations, Theses, and Capstone Projects

Male and female differences in frequency of occurrence in disease have perplexed scientists for some time. This in part derives from limitations in the systems in which one can evaluate sex differences. At the organismal level, differences can be hidden by a myriad of extensive and complex factors. Additional limitations exist since most biomedical studies are performed almost exclusively on male subjects, as the female hormonal milieu is intrinsically more variable and too troublesome for routine inclusion in research protocols. Research documenting sex differences continues to grow, and while most researchers suggests that sex hormones are at the core of …

Roles Of Igh Intronic Enhancer Eμ In Clonal Selection At The Pre-B To Immature B Cell Transition And In The Elimination Of Autoreactive B Cells, Cheng Peng

Dissertations, Theses, and Capstone Projects

The immunoglobulin heavy chain locus (Igh ) intronic enhancer, Eμ, enhances transcription of recombined Igh genes. We have previously shown that in mice with an Eμ-deficient Igh allele (V _H δ^a ), Igμ is expressed at half of the wild-type levels in pre-B cells. We also described an Eμ-dependent "check-point", operating at the pre-B to immature B cell transition, for heavy chain allelic exclusion. We now show that deletion of Eμ results in a smaller immature B cell compartment, and the pre-BCR/BCR signaling is diminished in pre-B cells as a result of the reduced Igμ levels, making it …

Quantitative Evaluation Of Microglial Activation And Vascularization In Suicide, Tatiana Pavlovna Schnieder

Dissertations, Theses, and Capstone Projects

Accumulated evidence points to immunological factors in psychiatric disorders. In a variety of chronic neurological disorders, exacerbation is associated with inflammation and a loss of integrity of the blood-brain barrier. Microglia, the principal brain immunological cells in the healthy state, respond to changes in the internal environment of the brain through a sequence of activated states. This study compared microglial phenotypes in the white matter of autopsy brains from 11 suicide victims and 25 subjects who died involuntarily. Both groups included cases with and without major psychiatric disorders, which were determined by PA interviews. Cases were matched for sex, age, …