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Choline Supplementation Normalizes Fetal Adiposity And Reduces Lipogenic Gene Expression In A Mouse Model Of Maternal Obesity, Chauntelle Jack-Roberts, Yaelle Joselit, Khatia Nanobashvili, Rachel Bretter, Olga V. Malysheva, Marie A. Caudill, Anjana Saxena, Kathleen Axen, Ahmed Gomaa, Xinyin Jiang

Publications and Research

Maternal obesity increases fetal adiposity which may adversely affect metabolic health of the offspring. Choline regulates lipid metabolism and thus may influence adiposity. This study investigates the effect of maternal choline supplementation on fetal adiposity in a mouse model of maternal obesity. C57BL/6J mice were fed either a high-fat (HF) diet or a control (NF) diet and received either 25 mM choline supplemented (CS) or control untreated (CO) drinking water for 6 weeks before timed-mating and throughout gestation. At embryonic day 17.5, HF feeding led to higher (p < 0.05) percent total body fat in fetuses from the HFCO group, while the choline supplemented HFCS group did not show significant difference versus the NFCO group. Similarly, HF feeding led to higher (p < 0.05) hepatic triglyceride accumulation in the HFCO but not the HFCS fetuses. mRNA levels of lipogenic genes such as Acc1, Fads1, and Elovl5, as well as the transcription factor Srebp1c that favors lipogenesis were downregulated (p < 0.05) by maternal choline supplementation in the HFCS group, which may serve as a mechanism to reduce fat accumulation in the fetal liver during maternal HF feeding. In summary, maternal choline supplementation improves indices of fetal adiposity in obese dams at late gestation.

Thyroid Function And Ischemic Heart Disease: A Mendelian Randomization Study, Jie V. Zhao, C. Mary Schooling

Publications and Research

To clarify the role of thyroid function in ischemic heart disease (IHD) we assessed IHD risk and risk factors according to genetically predicted thyroid stimulating hormone (TSH), free thyroxine (FT4) and thyroid peroxidase antibody (TPOAb) positivity. Separate-sample instrumental variable analysis with genetic instruments (Mendelian randomization) was used in an extensively genotyped case (n = 64,374)-control (n = 130,681) study, CARDIoGRAMplusC4D. Associations with lipids, diabetes and adiposity were assessed using the Global Lipids Genetics Consortium Results (n = 196,475), the DIAbetes Genetics Replication And Meta-analysis case (n = 34,380)-control (n = 114,981) study, and the Genetic Investigation of ANthropometric Traits (body …

Thermodynamic And Kinetic Analyses Of Iron Response Element (Ire)-Mrna Binding To Iron Regulatory Protein, Irp1, Mateen A. Khan, William E. Walden, Elizabeth C. Theil, Dixie J. Goss

Publications and Research

Comparison of kinetic and thermodynamic properties of IRP1 (iron regulatory protein1) binding to FRT (ferritin) and ACO2 (aconitase2) IRE-RNAs, with or without Mn2+, revealed differences specific to each IRE-RNA. Conserved among animal mRNAs, IRE-RNA structures are noncoding and bind Fe2+ to regulate biosynthesis rates of the encoded, iron homeostatic proteins. IRP1 protein binds IRERNA, inhibiting mRNA activity; Fe2+ decreases IRE-mRNA/IRP1 binding, increasing encoded protein synthesis. Here, we observed heat, 5 °C to 30 °C, increased IRP1 binding to IRE-RNA 4-fold (FRT IRE-RNA) or 3-fold (ACO2 IRE-RNA), which was enthalpy driven and entropy favorable. Mn2+ (50 μM, 25 °C) increased IRE-RNA/IRP1 …

Coding Of Velocity Storage In The Vestibular Nuclei, Sergei B. Yakushin, Theodore Raphan, Bernard Cohen

Publications and Research

Semicircular canal afferents sense angular acceleration and output angular velocity with a short time constant of ≈4.5 s. This output is prolonged by a central integrative network, velocity storage that lengthens the time constants of eye velocity. This mechanism utilizes canal, otolith, and visual (optokinetic) information to align the axis of eye velocity toward the spatial vertical when head orientation is off-vertical axis. Previous studies indicated that vestibular-only (VO) and vestibular-pause-saccade (VPS) neurons located in the medial and superior vestibular nucleus could code all aspects of velocity storage. A recently developed technique enabled prolonged recording while animals were rotated and …

Albright Uses Avatars, Horses To Improve Mental Health., Aldemaro Romero Jr.

Publications and Research

“When I was a college student, I was struggling in an intimate relationship I was forming, and it got to the point where I had to do something about it. So I went into the counseling center, and that’s what really turned me on—noticing the change within myself and the impact of counseling on the way we view our lives.”

That is the way Dr. Glenn Albright explains how he became interested in psychology in general and in counseling in particular. A native of Albany, New York, Albright received his bachelor’s in biology from Parson College in Iowa, his MS …

Sexual Dimorphism In Striatal Dopaminergic Responses Promotes Monogamy In Social Songbirds, Kirill Tokarev, Julia Hyland Bruno, Iva Ljubicic, Paresh J. Kothari, Santosh A. Helekar, Ofer Tchernichovski, Henning U. Voss

Publications and Research

In many songbird species, males sing to attract females and repel rivals. How can gregarious, non-territorial songbirds such as zebra finches, where females have access to numerous males, sustain monogamy? We found that the dopaminergic reward circuitry of zebra finches can simultaneously promote social cohesion and breeding boundaries. Surprisingly, in unmated males but not in females, striatal dopamine neurotransmission was elevated after hearing songs. Behaviorally too, unmated males but not females persistently exchanged mild punishments in return for songs. Song reinforcement diminished when dopamine receptors were blocked. In females, we observed song reinforcement exclusively to the mate’s song, although their …

Reduced Immune Investment With Energy Stress: Evidence From A Mouse Model And Human Studies, Alaina L. Schneider

Theses and Dissertations

During periods of energy stress, animals will prioritize and allocate energy from non-essential to essential tasks. Using a mouse model and published human data, we found that during periods of energy stress, energy is allocated away from immune function, and is prioritized for physical activity usage.

Sexual Dimorphism In The Femur And Pelvis Of Cebus Apella With A Randomization Experiment Examining Sample Size In The Fossil Record, Ryan P. Schaars

Theses and Dissertations

Measures of sexual dimorphism for six skeletal traits of the femur and pelvis of Cebus apella were calculated for 18 individuals to better understand primate skeletal dimorphism. A randomization experiment was also conducted on the measures of sexual dimorphism to examine sample size in the primate fossil record.

The Influence Of Phylogeny And Niche Differentiation On The Diets Of Malagasy Primates, Rebekka S. Hughes

Theses and Dissertations

Previous studies have shown that haplorhine diet is affected by phylogeny; however, until now studies in Malagasy strepsirrhines were lacking. The evolution of differences in Malagasy primates’ diets appears to differ from the pattern shown in haplorhines. My results indicate that niche differentiation may be a stronger predictor of diet.

Phylogenetic Affinities Of Homo Floresiensis Incorporating Postcranial Characters, Kristina M. Feeney

Theses and Dissertations

The objective of this analysis is to generate hypotheses regarding the evolution and ancestry of Homo floresiensis. Building on the previous study by Argue et al. (2009) by including a much larger sample of postcranial data for a more integrated picture of primitive and derived features across the skeleton.

“On Soulless Feet We Cross The Floor...” The Illusion Of Control In Grim Fandango And Virtual Spaces, Christina Cicchetti

Theses and Dissertations

Even though users can choose what they do in virtual spaces, they are forced to operate within the confines of realms designed by the invisible authors of the game’s code. The illusion of choice blinds users to an assessment of the hidden structure supporting immersive virtual worlds.

Native And Exotic Street Trees: A Spatio-Temporal Analysis In New York City, Mariana Schroter

Theses and Dissertations

This study looks at the differences in the temporal distribution of urban street native and exotic trees in New York City from 1995 to 2015, attempting to answer the research question: Is there inequity in the distribution of tree population diversity or health, affected by socioeconomic and physical factors, when nested within origin?

Effects Of Fluoxetine On Social And Startle Behavior In The African Cichlid Astatotilapia Burtoni, Stephanie Shih

Theses and Dissertations

The commonly prescribed antidepressant Prozac (fluoxetine) is found in waste water and affects aquatic animals. Here we ask how social hierarchy and startle behavior in an African cichlid fish community is impacted by chronic fluoxetine exposure. Results indicate reduced aggression and startle rates, implying possible ecological consequences.

Feline Object Play: The Influence Of Catnip, Keri Detorres

Theses and Dissertations

Catnip triggers a unique olfactory response and results in a play-like activity in felines. This invoked response appears similar to, but more exaggerated and intense, than regular play. The play behavior of 24 cats with two identical toys, one of which was infused with catnip, was compared.

Methamphetamine-Induced Conditioned Place Preference In Adolescent Male And Female Mice Of Two Strains, Andre Bevil Toussaint

Theses and Dissertations

We used a conditioned place preference paradigm to test the rewarding effects of methamphetamine (1mg/kg) in adolescent male and female mice of two strains. We found that methamphetamine induced conditioned place preference in female C57Bl/6 and male 129/SvEv mice, but not female 129/SvEv or male C57Bl/6 mice.

Quantifying The Development Of Usergenerated Art During 2001-2010, Mehrdad Yazdani, Jay Chow, Lev Manovich

Publications and Research

One of the main questions in the humanities is how cultures and artistic expressions change over time. While a number of researchers have used quantitative computational methods to study historical changes in literature, music, and cinema, our paper offers the first quantitative analysis of historical changes in visual art created by users of a social online network. We propose a number of computational methods for the analysis of temporal development of art images. We then apply these methods to a sample of 270,000 artworks created between 2001 and 2010 by users of the largest social network for artÐDeviantArt (www.deviantart.com). We …

Domestic Cat Ethology: Evaluating Social Media For Data Collection, Patricia Casey

Theses and Dissertations

This study looked at whether user-submitted videos of cats could be used to increase understanding of their behavior. The findings suggest that the behaviors observed were consistent with prior research, and contain typical cat behavior. Depending on the research question, user-submitted videos of cats are a promising data source.

Retrotransposons Are The Major Contributors To The Expansion Of The Drosophila Ananassae Muller F Element, Wilson Leung, Gerard Mcneil

Publications and Research

The discordance between genome size and the complexity of eukaryotes can partly be attributed to differences in repeat density. The Muller F element (5.2 Mb) is the smallest chromosome in Drosophila melanogaster, but it is substantially larger (.18.7 Mb) in D. ananassae. To identify the major contributors to the expansion of the F element and to assess their impact, we improved the genome sequence and annotated the genes in a 1.4-Mb region of the D. ananassae F element, and a 1.7-Mb region from the D element for comparison. We find that transposons (particularly LTR and LINE retrotransposons) are major contributors …

Zero Textbook Cost Syllabus For Plsc 2260 (Introduction To Comparative Government), Anh Tran

Open Educational Resources

Why do states wield violence against its citizens? Does the expansion of state power always threaten individual freedom? When do ordinary people create social change peacefully, and when do they go to war? Is democratization an inevitable force in our world? Is there a trade-off between economic growth and economic equality? These are the types of puzzles we will be exploring through various theoretical and methodological lenses. We will compare variations in political behaviors, processes, and structures at work in different countries around the world. Each week, we focus on a different topic in comparative politics, then dig deeper into …

Regulating Anger Under Stress Via Cognitive Reappraisal And Sadness, Jun Zhan, Xiaofei Wu, Jin Fan, Jiayou Guo, Jianshe Zhou, Jun Ren, Chang Liu, Jing Luo

Publications and Research

Previous studies have reported the failure of cognitive emotion regulation (CER), especially in regulating unpleasant emotions under stress. The underlying reason for this failure was the application of CER depends heavily on the executive function of the prefrontal cortex (PFC), but this function can be impaired by stress-related neuroendocrine hormones. This observation highlights the necessity of developing selfregulatory strategies that require less top-down cognitive control. Based on traditional Chinese philosophy and medicine, which examine how different types of emotions promote or counteract one another, we have developed a novel emotion regulation strategy whereby one emotion is used to alter another. …

Pulling The Plug On Microscopes In The Anatomy And Physiology Laboratory, Zvi Ostrin, Vyacheslav Dushenkov

Publications and Research

Virtual microscopy (VM) has been widely available for more than a decade, especially in clinical settings and medical schools. In recent years the movement away from conventional optical microscopy (OM) and towards VM has been accelerating and several VM websites are now available online and readily accessible to educators. VM can enhance the instructor’s ability to teach the histology component of anatomy and physiology classes, facilitate student learning, save time for both students and instructors, and ultimately save money that can be more productively used for other facets of the laboratory. The many pedagogical and practical advantages provided by VM …

Sibrava Studies, Teaches About Stress And Anxiety, Aldemaro Romero Jr.

Publications and Research

“There’s a cliché answer that you get from a lot of folks who say, ‘Well, I’ve always been interested in people,’ and that is true, but for me I always fan- tasized about being a lawyer when I grew up.” This is how Dr. Nicholas Sibrava explains his choice of career. In fact, he envisioned himself in a courtroom, just like in the movies.

It also explains why this native of Cleveland, Ohio obtained a bachelor’s degree in both criminology and psychology. The psychology part crept up on him while taking some psych courses. “Somewhere along the way I flip-flopped …

Race, Gender Play Role In College Graduations, Aldemaro Romero Jr.

Publications and Research

One of the best ways to measure the accomplishment of colleges and universities is by assessing the percentage of their undergraduate students who graduate within six years of enrollment. Although most undergraduate degrees are designed to be completed in four years, the norm is to count grad- uation rates in six-year intervals because many students have to work (many of them full-time), while others do not have the adequate preparation from high school to succeed and need more time to overcome their academic shortcomings. There are many factors that can delay time to graduation.

Many states tie funding for their …

T-Dna-Genome Junctions Form Early After Infection And Are Influenced By The Chromatin State Of The Host Genome, Shay Shilo, Pooja Tripathi, Cathy Melamed Bessudo, Oren Tzfadia, Theodore R. Muth, Avraham A. Levy

Publications and Research

Agrobacterium tumefaciens mediated T-DNA integration is a common tool for plant genome manipulation. However, there is controversy regarding whether T-DNA integration is biased towards genes or randomly distributed throughout the genome. In order to address this question, we performed high-throughput mapping of T-DNA-genome junctions obtained in the absence of selection at several time points after infection. T-DNA-genome junctions were detected as early as 6 hours post-infection. T-DNA distribution was apparently uniform throughout the chromosomes, yet local biases toward AT-rich motifs and T-DNA border sequence micro-homology were detected. Analysis of the epigenetic landscape of previously isolated sites of T-DNA integration in …

Chelator-Free Radiolabeling Of Serrs Nanoparticles For Whole-Body Pet And Intraoperative Raman Imaging, Matthew A. Wall, Travis Shaffer, Stefan Harmsen, Darjus-Felix Tschaharganeh, Chun-Hao Huang, Scott W. Lowe, Charles Michael Drain, Moritz F. Kircher

Publications and Research

A single contrast agent that offers whole-body non-invasive imaging along with the superior sensitivity and spatial resolution of surface-enhanced resonance Raman scattering (SERRS) imaging would allow both pre-operative mapping and intraoperative imaging and thus be highly desirable. We hypothesized that labeling our recently reported ultrabright SERRS nanoparticles with a suitable radiotracer would enable pre-operative identification of regions of interest with whole body imaging that can be rapidly corroborated with a Raman imaging device or handheld Raman scanner in order to provide high precision guidance during surgical procedures. Here we present a straightforward new method that produces radiolabeled SERRS nanoparticles for …

Maternal Choline Supplementation Alters Fetal Growth Patterns In A Mouse Model Of Placental Insufficiency, Julia H. King, Sze Ting (Cecilia) Kwan, Jian Yan, Kevin C. Klatt, Xinyin Jiang, Mark S. Roberson, Marie A. Caudill

Publications and Research

Impairments in placental development can adversely affect pregnancy outcomes. The bioactive nutrient choline may mitigate some of these impairments, as suggested by data in humans, animals, and human trophoblasts. Herein, we investigated the effects of maternal choline supplementation (MCS) on parameters of fetal growth in a Dlx3+/− (distal-less homeobox 3) mouse model of placental insufficiency. Dlx3+/− female mice were assigned to 1X (control), 2X, or 4X choline intake levels during gestation. Dams were sacrificed at embryonic days E10.5, 12.5, 15.5, and 18.5. At E10.5, placental weight, embryo weight, and placental efficiency were higher in 4X versus 1X choline. Higher concentrations …

Pixuna Virus Modifies Host Cell Cytoskeleton To Secure Infection, Pedro Ignacio Gil, Guillermo Albrieu-Llinás, Estela Cecilia Mlewski, Marina Monetti, Laura Fozzatti, Cecilia Cuffini, José Fernández-Romero, Patricia Kunda, María Gabriela Paglini

Publications and Research

Pixuna virus (PIXV) is an enzootic member of the Venezuelan Equine Encephalitis Virus complex and belongs to the New World cluster of alphaviruses. Herein we explore the role of the cellular cytoskeleton during PIXV replication. We first identified that PIXV undergoes an eclipse phase consisting of 4 h followed by 20 h of an exponential phase in Vero cells. The infected cells showed morphological changes due to structural modifications in actin microfilaments (MFs) and microtubules (MTs). Cytoskeletonbinding agents, that alter the architecture and dynamics of MFs and MTs, were used to study the role of cytoskeleton on PIXV replication. The …

Quantifier Spreading In Child Eye Movements: A Case Of The Russian Quantifier Kazhdyj ‘Every’, Irina A. Sekerina, Antje Sauermann

Publications and Research

Extensive cross-linguistic work has documented that children up to the age of 9–10 make errors when performing a sentence-picture verification task that pairs spoken sentences with the universal quantifier every and pictures with entities in partial one-to-one correspondence. These errors stem from children’s difficulties in restricting the domain of a universal quantifier to the appropriate noun phrase and are referred in the literature as quantifier-spreading (q-spreading). We adapted the task to be performed in conjunction with eye-movement recordings using the Visual World Paradigm. Russian-speaking 5-to-6-year-old children (N = 31) listened to sentences like Kazhdyj alligator lezhit v vanne ‘Every alligator …

Burden Of Atopic Dermatitis In The United States: Analysis Of Healthcare Claims Data In The Commercial, Medicare, And Medi-Cal Databases, Sulena Shrestha, Raymond Miao, Li Wang, Jingdong Chao, Huseyin Yuce, Wenhui Wei

Publications and Research

Comparative data on the burden of atopic dermatitis (AD) in adults relative to the general population are limited. We performed a large-scale evaluation of the burden of disease among US adults with AD relative to matched non-AD controls, encompassing comorbidities, healthcare resource utilization (HCRU), and costs, using healthcare claims data. The impact of AD disease severity on these outcomes was also evaluated.

The Living Syllabus: Rethinking The Introductory Course To Art History With Interactive Visualization, Caroline Bruzelius, Hannah L. Jacobs

Art History Pedagogy & Practice

This essay describes an experiment in adopting mapping and timeline technologies in the Introduction to Art History course taught at Duke University. The creation of an interactive, “living,” syllabus in Neatline and Omeka allowed us to embed maps, course powerpoints, links to museum websites, news articles, videos, and clips from movies. In this article, we describe how the integration of mapping tools and multimedia transformed our approach to the discipline of Art History, enabling us to engage with trade and exchange networks for raw materials, artistic ideas and motifs, and the art market.