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Chloride Intracellular Channel Proteins Respond To Heat Stress In Caenorhabditis Elegans, Jun Liang, Yakov Shaulov, Cathy Savage-Dunn, Stéphane Boissinot, Tasmia Hoque

Publications and Research

Chloride intracellular channel proteins (CLICs) are multi-functional proteins that are expressed in various cell types and differ in their subcellular location. Two CLIC homologs, EXL-1 (excretory canal abnormal like-1) and EXC-4 (excretory canal abnormal± 4), are encoded in the Caenorhabditis elegans genome, providing an excellent model to study the functional diversification of CLIC proteins. EXC-4 functions in excretory canal formation during normal animal development. However, to date, the physiological function of EXL-1 remains largely unknown. In this study, we demonstrate that EXL-1 responds specifically to heat stress and translocates from the cytoplasm to the nucleus in intestinal cells and body …

Maternal Hypothalamic-Pituitary-Adrenal Axis Response To Foraging Uncertainty: A Model Of Individual Vs. Social Allostasis And The "Superorganism Hypothesis", Jeremy D. Coplan, Nishant K. Gupta, Asif Karim, Anna Rozenboym, Eric L.P. Smith, John G. Kral, Leonard A. Rosenblum

Publications and Research

Introduction: Food insecurity is a major global contributor to developmental origins of adult disease. The allostatic load of maternal food uncertainty from variable foraging demand (VFD) activates corticotropin-releasing factor (CRF) without eliciting hypothalamic-pituitary-adrenal (HPA) activation measured on a group level. Individual homeostatic adaptations of the HPA axis may subserve second-order homeostasis, a process we provisionally term “social allostasis.” We postulate that maternal food insecurity induces a “superorganism” state through coordination of individual HPA axis response.

Methods: Twenty-four socially-housed bonnet macaque maternal-infant dyads were exposed to 16 weeks of alternating two-week epochs of low or high foraging demand shown to compromise …

Combined Mnemonic Strategy Training And High-Definition Transcranial Direct Current Stimulation For Memory Deficits In Mild Cognitive Impairment, Benjamin M. Hampstead, Krishnankutty Sathian, Marom Bikson, Anthony Y. Stringer

Publications and Research

Introduction: Memory deficits characterize Alzheimer’s dementia and the clinical precursor stage known as mild cognitive impairment. Nonpharmacologic interventions hold promise for enhancing functioning in these patients, potentially delaying functional impairment that denotes transition to dementia. Previous findings revealed that mnemonic strategy training (MST) enhances long-term retention of trained stimuli and is accompanied by increased blood oxygen level–dependent signal in the lateral frontal and parietal cortices as well as in the hippocampus. The present study was designed to enhance MST generalization, and the range of patients who benefit, via concurrent delivery of transcranial direct current stimulation (tDCS).

Methods: This protocol describes …

Bacterial Diversity Impacts As A Result Of Combined Sewer Overflow In A Polluted Waterway, Olga Calderón, Holly Porter-Morgan, Joby Jacob, Willis Elkins

Publications and Research

Newtown Creek is an industrial waterway and former tidal wetland in New York City. It is one of the most polluted water bodies in the United States and was designated as a superfund site in 2010. For over a century, organic compounds, heavy metals, and other forms of industrial pollution have disrupted the creek’s environment. The creek is also impacted by discharges from twenty combined sewer overflow pipes, which may deposit raw sewage or partially treated wastewater directly into the creek during heavy or sustained rain events. Combined sewer overflow events and associated nutrient over-enrichment at the creek drive eutrophication …

Synthesis And Evaluation Of Tetrahydroprotoberberines As Dopamine Receptor Ligands, Satishkumar V. Gadhiya

Dissertations, Theses, and Capstone Projects

Dopamine (DA) receptors belong to the G-protein coupled receptors (GPCRs) family, divided in to two groups based on their high homology transmembrane domains; D1-like DA receptors (D1, D5) and D2-like DA receptors (D2-D4). DA receptor specific ligands have been exploited as a means for studying the prognosis and curing several CNS disorders. Though several efforts have been devoted to discover selective and potent DA receptor ligands, complete selectivity within the DA receptor subtypes remains a challenge.

Tetrahydroprotoberberines (THPBs) are a group of naturally occurring tetracyclic alkaloids that belong to the tetrahydroisoquinoline family. A wide range of biological activities are associated …

Community, Identity, And Tradition Within A Progressive Christian Congregation, James W. Skinner

Dissertations, Theses, and Capstone Projects

Trends towards religious individualism, the de-institutionalization of religion, and the decline of denominational affiliations potentially impact religious congregations in a range of different ways. Drawing on a variety of theoretical and historical perspectives, this study examines these issues through a qualitative case study of a progressive Christian congregation in Brooklyn, New York. The case study explores the history, worship practices, and culture of the congregation in detail, focusing on the formation of religious identity and community within the context of congregational life. This close examination of the culture of the congregation reveals the ways in which the tensions between religious …

Desisting In Prison: Myth And The Council For Unity Model, Kevin Moran

Dissertations, Theses, and Capstone Projects

This dissertation is a qualitative examination of aspects of the desistance process among incarcerated men in both prison and jail. Data collection for this project occurred in and around the correctional version of the Council For Unity program, which is also examined in this write up. The premise of this project is that a minority of men do desist whilst incarcerated and thus the research presented here analyzes how prisoners act towards their attempts to desist from crime in terms of the meaning this process has for them, their interaction with others during this process, and the interpretative progression by …

Semantic Attention: Effects Of Modality, Lexicality And Semantic Content, David Britton

Dissertations, Theses, and Capstone Projects

Since the discovery of the Stroop Effect in 1935 questions about the role of language vs. non-lexical stimuli in selective attention remain. Early researchers attributed the powerful distracting influence shown in the Stroop task, naming the color in which a spelled word is printed when incongruent with the color name the word spells, to an automaticity of language that gives it privileged access to meaning, but many others since have shown various ways to reduce or even reverse this distracting effect of an incongruent word. This study addresses this by using EEG to record neural activity along with reaction time …

The Nucleus Accumbens Core Dopamine D1 And Glutamate Ampa/Nmda Receptors Play A Transient Role In The Performance Of Pavlovian Approach Behavior, Veronica Dobrovitsky

Dissertations, Theses, and Capstone Projects

The role of the nucleus accumbens core (NAc core) continues to be redefined with newly acquired data on neurochemical mechanisms mediating the learning and performance of behavior. Previous empirical data showed that dopamine transmission at the D1 receptor (D1R) plays a transient role in the expression of learned Pavlovian approach behavior. Here we show that, prior to overtraining, dopamine activity at D1Rs specifically within the NAc core is critical for the performance of approach behavior elicited by the recently-acquired reward-paired cue. Blockade of D1Rs in the NAc core, but not the dorsomedial striatum or NAc shell, disrupted approach responses during …

Comparative Phylogeographic, Population Genomic, And Selection Inference With Development Of Hierarchical Co-Demographic Models, Alexander Xue

Dissertations, Theses, and Capstone Projects

Comparing demographic histories across assemblages of populations, species, and sister pairs has been a focus in phylogeography since its inception. Initial approaches utilized organelle genetic data and involved qualitative comparisons of genetic patterns for evaluating hypotheses of shared evolutionary responses to past environmental changes. This endeavor has progressed with coalescent model-based statistical techniques and advances in next-generation sequencing, yet there remains a need for methods that can analyze aggregated genomic-scale data from non-model organisms within a unified framework that considers individual taxon uncertainty and variance. To this end, the aggregate site frequency spectrum (aSFS), an expansion of the site frequency …

The Contributions Of Child And Romantic Attachment To Sleep Quality, Karen Estefane

Dissertations, Theses, and Capstone Projects

Little is known about the relation between attachment and sleep, although both develop around the first year of life and mutually influence each other. Furthermore, attachment styles and dimensions have been associated with sleep difficulties in both childhood and adulthood. However, these findings have not been consistent, especially among dismissing individuals. In addition, the specific contributions of both child and romantic attachment to sleep quality have not been investigated thus far. This cross-sectional study aims to examine the ways child and romantic attachment are associated with sleep quality. A sample of 671 heterosexual residents of the United States, aged 18 …

Bodies Of Resistance: On (Not) Naming Gender In The Medieval West, Alexander V. Baldassano

Dissertations, Theses, and Capstone Projects

This dissertation considers the genres of historiography, romance, hagiography, Chaucerian poetry, and court transcripts. While there are no extant manuscripts depicting transgender-like people’s accounts of themselves, literature of the Middle Ages is replete with fictionalized depictions of ambiguously or transgressively gendered individuals who are meant to symbolize or represent something other than themselves. By investigating how a variety of genres depicts sensationalized and transgressively gendered embodiments, I examine the presentation of transgender-like subjectivity as a manipulation of rhetoric. Viviane Namaste critiques theory such as Marjorie Garber’s Vested Interests, claiming that it reduces the transvestite figure to a rhetorical trope …

Psychoanalysis And Modernity: A Failure To Find Relief From Existential Terror, Erin Liat Claridge

Dissertations, Theses, and Capstone Projects

This project considers the ways in which culture—the symbols and institutions that guide behavior and provide meaning to the individuals living within a community—serves a defensive function against conscious awareness of existential terror. The term existential terror refers to the cognitive and emotional experience of recognizing the inevitability of death, which is often accompanied by feelings of angst, isolation from others, and awareness of meaninglessness. In this dissertation, I will argue that the broad shift from traditional culture based on Judeo-Christian religious beliefs and communal forms of social life to modernity represents a slow but destabilizing deterioration of the defensive …

Thule Iron Use In The Pre-Contact Arctic, Eileen Colligan

Dissertations, Theses, and Capstone Projects

This thesis examines the use of iron by the Thule people, a Neoeskimo culture that lived in the North American Arctic between approximately 1000 AD and 1400 ̶ 1500 AD. The study takes a pan-Arctic perspective to bring together research that has usually been done on a more-limited geographical scale. This viewpoint shows the Thule culture from a view that corresponds to their world.

The study focuses on: (1) revisions in the accepted chronology of the Thule and how these have affected the explanations for the Thule Migration from Alaska to Greenland; (2) new understandings about the iron that was …

Bodies Of Knowledge: An Anatomy And Kinesiology Of The American Prison Nation, 'Human'-Making, And Twenty-First-Century Techno-Gods, Lyndsey Karr

Dissertations, Theses, and Capstone Projects

The social production of hegemonic knowledge has historically been legitimized in relation to the sanctioned status of the ‘Human’.[1] Beginning with the American Prison Industrial Complex and what sociologist Beth E. Richie conceptualizes as the “prison nation,” I will show the ‘human’ as a contingent and composite status appearing along a spectrum of Flesh, Body, and ‘Human’ (Flesh-Body-‘Human’) statuses and subjectivity.

Bringing this ‘Human’ continuum into conversation with twenty-first-century media, (micro)computational technologies, and contemporary knowledge and social economies, I expand the notion, reach, and scale of the American “prison nation.” Following Mark Hansen’s treatment of twenty-first-century digital media, I …

Roles Of Gabab, Muscarinic And Nicotinic Receptor Signaling In The Acquisition And Expression Of Fructose And Fat-Conditioned Flavor Preferences And Acquisition Of Quinine-Conditioned Flavor Avoidances In Rats, Francis M. Rotella

Dissertations, Theses, and Capstone Projects

In addition to increased intake of sweet solutions by mammals, learning, particularly classically-conditioned “Pavlovian-like” learning, also plays an important role. An orosensory conditioned flavor preference (CFP) can be elicited by pairing one novel flavor (conditioned stimulus, CS+) with a fructose solution and a second novel flavor (CS-) with a saccharin solution. Rats will prefer the CS+ flavor in a subsequent 2-bottle choice test with both flavors mixed in saccharin. Previous pharmacological analyses revealed that systemic administration of dopamine (DA) D1 and D2 as well as NMDA, but not opioid, receptor antagonists eliminated the acquisition (learning) of fructose-CFP. Further, expression of …

Parallels Of Love And Hate Defined By Attachments, Nicole Spann

Dissertations, Theses, and Capstone Projects

The assertion, “It’s a thin line between love and hate,” is often referenced to express the relative ease of a sole emotion morphing from one extreme to next. Both love and hate generate the same physiological fluctuations which include, but are not limited to, a racing pulse, sweaty palms, releasing of hormones, and some degree of excitability. While physiologically the responses are the same, psychologically and philosophically they are considered opposites. In the sphere of psychology, it is going from what is considered normal behavior or a normal response to abnormal behavior or an abnormal response (McKay, 2016). For this …

Morphogenesis And Growth Driven By Selection Of Dynamical Properties, Yuri Cantor

Dissertations, Theses, and Capstone Projects

Organisms are understood to be complex adaptive systems that evolved to thrive in hostile environments. Though widely studied, the phenomena of organism development and growth, and their relationship to organism dynamics is not well understood. Indeed, the large number of components, their interconnectivity, and complex system interactions all obscure our ability to see, describe, and understand the functioning of biological organisms.

Here we take a synthetic and computational approach to the problem, abstracting the organism as a cellular automaton. Such systems are discrete digital models of real-world environments, making them more accessible and easier to study then their physical world …

A Combined Computational Strategy Of Sequence And Structural Analysis Predicts The Existence Of A Functional Eicosanoid Pathway In Drosophila Melanogaster, Michael Scarpati

Dissertations, Theses, and Capstone Projects

With increased understanding of their roles in signal transduction and metabolism, eicosanoids have emerged as important players in human health and disease. Mammalian prostanoids and related lipid mediators perform varied functions in different tissues and organs. Synthesized through the oxygenation of C20 polyunsaturated fatty acids, mammalian eicosanoids are both pro- and anti-inflammatory. The physiological contexts in which eicosanoid family members act at the cellular level are not well understood. In this study, we examined whether the genome of Drosophila melanogaster, a powerful model for innate immunity and inflammation, codes for the enzymes required for eicosanoid biosynthesis. We report the …

Modulation Of Spasticity By Trans-Spinal Direct Current Stimulation In Animals With Spinal Cord Injury, Wagdy Mekhael

Dissertations, Theses, and Capstone Projects

Central nervous system injuries usually produce motor impairments that are exacerbated by pathologically altered muscle tone. Abnormal muscle tone interferes with voluntary movement and is associated with loss of dexterity. Prior work in our laboratory demonstrated that 30-second trans-spinal direct current (DC) stimulation can temporarily modify muscle tone in anesthetized spastic mice after spinal cord injury (SCI). These experiments described DC-induced muscle tone responses to be polarity-dependent. That is, anodal stimulation (current passed from the lumbar spine to sciatic nerve) decreased muscle tone, and cathodal stimulation (current passed from the sciatic nerve to the lumbar spine) increased it. The present …

Capture And Abandon: Social Reproduction And Informal Land Tenure In Jamaica, Rachel Goffe

Dissertations, Theses, and Capstone Projects

Capture and Abandon: Social Reproduction and Informal Land Tenure in Jamaica examines how ongoing policy development to curtail squatting is shifting state capacity away from a project of land reform and towards one of land management. Scholarship about informal settlements elsewhere tends to understand dispossession as a project of the neoliberal state. I argue that it is strategically necessary to elucidate the ways in which the production of durable but insecure access to land is not novel, but is imbricated in the historical production of a Black labor force that is self-sufficient and yet ready at hand, reproducing themselves through …

Mechanisms Of Navigation In Fiddler Crabs: An Analysis Of Allocentric And Egocentric Contributions, Stephen G. Volz

Dissertations, Theses, and Capstone Projects

Navigation in biological systems is a complex task-set that involves learning processes and may include constructing representations of features of their environment. Across the animal kingdom, different learning mechanisms have evolved to similar spatial problems. The extent to which mechanisms are conserved across taxa are an important research area that can guide our understanding of the cognitive dimensions of navigation. Recent studies of mammals, birds, and arthropods has found that these animals often attend to multiple forms of sensory cues, and to either integrate the solutions generated by these cues, or at times prefer one form of cue over another. …

Albert Camus's Mediterraneanism In La Peste, Jacquelyn Libby

Dissertations, Theses, and Capstone Projects

The following dissertation uses the speech Camus gave at the inauguration of a new community arts center in Algiers in 1937, entitled “La Culture Indigène. La Nouvelle Culture Méditerranéenne,” to show that this expression of Mediterraneanism, as well as its evolution, can be detected in his novel La Peste, which was published ten years later in 1947. The bearing of Camus’s identification with the Mediterranean world on his speech is that it generated a sense of in-betweenness. This in-betweenness, I argue, is a key feature of Camus’s Mediterranean thought that is also found in La Peste’s subject matter, …

Climate-Driven Habitat Shifts In South America: Biogeography, Historical Demography, And Population Genomics Of Anole Lizards, Ivan Prates

Dissertations, Theses, and Capstone Projects

Shifts in the geographic distribution of habitats over time can promote dispersal and vicariance, thereby influencing large-scale biogeographic patterns and ecological processes. The establishment of corridors of suitable habitat across previously disjunct yet ecologically similar regions has been widely linked to climate change over time. Such climate-mediated habitat changes may have played a key role in the assembly of tropical biotas, including those of Amazonia and the coastal Atlantic Forest in South America, two of the most diverse ecosystems on Earth. The history of biogeographic associations between tropical rainforest habitats in response to former climatic shifts, as well as their …

The Role Of T-Box Proteins In Vertebrate Germ Layer Formation And Patterning, Sushma Teegala

Dissertations, Theses, and Capstone Projects

All of the tissues in triploblastic organisms, with the exception of the germ cells, arise from the three germ layers, ectoderm, mesoderm and the endoderm. The identification of the genes that underlie the differentiation of these layers is crucial to our understanding of development. T-box family proteins are DNA-binding transcriptional regulators that play important roles during germ layer formation in the early vertebrate embryo. Well-characterized members of this family, including the transcriptional activators Brachyury and VegT, are essential for the proper formation of mesoderm and endoderm, respectively. To date, T-box proteins have not been shown to play a role in …

You Need Violence And Sincerity: Godard And The Manufacturing Of A Revolutionary Cinema, Matthew Ballinger

Dissertations, Theses, and Capstone Projects

Godard’s cinema of the mid-to-late-sixties offers a compelling body of work in which we can witness the director making a more conscious effort to renounce the tendencies of what Peter Wollen terms ‘orthodox cinema,’ through a more disruptive and textual approach, which, of course, mirrored his increasingly radical politics. This attempt to marry politics and art raises the question of what a genuinely revolutionary cinema would look like, or if it is even possible at all. This thesis will attempt to tackle just such a question, discussing three of Godard’s films from the period to examine this evolving radical tendency: …

Trypanosome Lytic Factor Mediated Immunity Against Leishmania Sp., Jyoti Pant

Dissertations, Theses, and Capstone Projects

Trypanosome Lytic Factor (TLF) is an innate immunity complex that was originally discovered to protect against African Trypanosomes. The major components of TLF are Apolipoprotein A1 (APOA1), Apolipoprotein L1 (APOL1) and HPR (Haptoglobin Related Protein), where APOL1 is necessary and sufficient for trypanolysis. Recently we have shown that TLF ameliorates infections by cutaneous Leishmania species. Here we investigated the effect of different primate and human TLF against different Leishmania sp. Our result shows that TLF kills metacyclic promastigotes of cutaneous Leishmania sp. within immune cells such as neutrophils and macrophages by two different mechanism. Using transiently transfected and germline transgenic …

Sacralizar Lo Efímero: Configuración Del Amante Y El Amado En Luis Cernuda Y César Moro, Lena R. Retamoso Urbano

Dissertations, Theses, and Capstone Projects

In this study I create a dialogue between two poets, Luis Cernuda from Spain and César Moro from Peru. I use two of their most representative books of poetry, Cernuda’s Los placeres prohibidos and Moro’s La tortuga ecuestre, to study the dialectics that take place between the lover and the beloved. My general hypothesis is that Los placeres prohibidos can be read as a prologue to the lyrical discourse of La tortuga ecuestre. To explore the bridge between these two poetic worlds, I analyze the various mechanisms through which Eros expresses itself.

Drawing on the work of Denis …

The Effects Of Pre-Trial Event Stimulus Properties On Timing In The Peak Interval Procedure, Daniel A. Garces

Dissertations, Theses, and Capstone Projects

In the peak interval procedure, intruded conditioned stimuli produce shifts in peak/middle time towards later values, regardless of whether these stimuli are presented prior to or during the timing signal. Although the effects of during-trial stimulus properties—temporal location, duration, and salience—have been previously reported, no research exists on how before-trial stimulus properties influence the extent of shifts in middle time. In the present study, we manipulated within subjects both the temporal location and type (i.e., cue alone, response-independent reinforcer alone, or cue and response-contingent reinforcer together) of the pre-trial event. An individual-trial analysis suggested that the type of stimulus event …

Aberrant Salience And Probabilistic Reasoning In Distressing Attenuated Positive Psychotic Symptoms: An Examination Of A Two Factor Model, Huw Green

Dissertations, Theses, and Capstone Projects

Theoretical psychological models of positive psychotic symptoms have increasingly emphasized the interaction of multiple cognitive factors. Research into delusions in particular has focused on the interaction of two factors; a perceptual anomaly that gives rise to a need for explanation, and a bias toward premature acceptance of a hypothesis. Recently this two factor approach has been applied to positive psychotic symptoms more broadly. Two candidate factors have received extensive theoretical and empirical interest. The aberrant salience hypothesis posits that salience regulation, mediated by dopamine, goes awry in psychosis, giving rise to a generalized sense of undue significance that is applied …