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Chulalongkorn University

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Articles 1951 - 1980 of 37618

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An Accidental Discovery Of Ductal Adenocarcinoma Of The Pancreas In An Adult Horse With Severe Abdominal Pain, Thita Wonghanchao, Kanokpan Sanigavatee, Chanoknun Poochipakorn, Onjira Huangsaksri, Phudit Maneesaay, Rungrueang Yodsheewan, Metha Chanda Aug 2024

An Accidental Discovery Of Ductal Adenocarcinoma Of The Pancreas In An Adult Horse With Severe Abdominal Pain, Thita Wonghanchao, Kanokpan Sanigavatee, Chanoknun Poochipakorn, Onjira Huangsaksri, Phudit Maneesaay, Rungrueang Yodsheewan, Metha Chanda

The Thai Journal of Veterinary Medicine

A native pony, eight years old and male, was tentatively diagnosed with abdominal pain and treated immediately. Sequential hematological profiles revealed increased levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP), and creatinine, as well as reduced albumin. After the pony's death, which occurred eight days after treatment began, a necropsy was performed. The liver's external surface showed small foci, but no gross lesion was observed in the pancreas, which was enlarged. Haematoxylin and eosin (H&E) staining of the liver showed multifocal inflammation in the hepatic parenchyma, coinciding with bile duct hyperplasia. The pancreas H&E staining showed well-differentiated tubular glands with …


A Conjunctival Mast Cell Tumor In Golden Retriever: A Case Report, Orapun Jaturakan Aug 2024

A Conjunctival Mast Cell Tumor In Golden Retriever: A Case Report, Orapun Jaturakan

The Thai Journal of Veterinary Medicine

A high-grade mast cell tumor of the conjunctiva in the Golden Retriever is a rare condition. Wide excisional conjunctival mass biopsy is suggested, combined with chemotherapy. A 9-year-old neutered female Golden Retriever with a painless conjunctival mass at the right temporal bulbar conjunctiva was presented at the Ophthalmology Clinic of the Small Animal Teaching Hospital, Faculty of Veterinary Sciences, Chulalongkorn University. Complete physical and ophthalmic examinations were performed. The metastasis of the primary conjunctival mass was investigated by radiography of the skull, orbit, thorax, and abdomen, as well as by ultrasonography in the orbit and abdomen. No metastasis was confirmed …


Cervical Lymphangiosarcoma In A Poodle Dog: Surgical Treatment And Chemotherapy, Minkyung Kim, Young-Hwan Kim, Jae-Eun Hyun Aug 2024

Cervical Lymphangiosarcoma In A Poodle Dog: Surgical Treatment And Chemotherapy, Minkyung Kim, Young-Hwan Kim, Jae-Eun Hyun

The Thai Journal of Veterinary Medicine

A 7-year-old neutered male Poodle exhibited a persistent caudoventral cervical mass with draining lesions characterized by chronic and relapsing features. Upon presentation, the mass measured 7 × 11 × 11.5 cm, containing sanguine fluid. Computed tomography revealed the diffuse extension of the cervical mass from the C3 level to the thoracic inlet, affecting the sternal lymph node. Extensive surgical removal was attempted with meticulous resection to avoid damaging the right jugular vein, carotid sheath, and vagus nerve within the mass. Histopathology and immunohistochemical staining for factor VIII confirmed the diagnosis of lymphangiosarcoma. Due to inadequate surgical margin excision, four cycles …


Food Safety, Swine Health, And Productivity Of A Farrow-To-Finish Swine Farm Following Implementation Of A Certified Haccp System In Japan, Hiroshi Akaike, Makoto Nagai, Tomomitsu Alexandre Okatani, Sumalee Boonmar, Yukio Morita Aug 2024

Food Safety, Swine Health, And Productivity Of A Farrow-To-Finish Swine Farm Following Implementation Of A Certified Haccp System In Japan, Hiroshi Akaike, Makoto Nagai, Tomomitsu Alexandre Okatani, Sumalee Boonmar, Yukio Morita

The Thai Journal of Veterinary Medicine

This study examined the safety and productivity of a farrow-to-finish swine farm from 2015 to 2019, in Japan. The farm implemented a farm Hazard Analysis and Critical Control Points (HACCP) system in July 2016. The critical control point was “the selection of shipping pigs” and the critical limit for preventing contamination with residual antibiotics in pigs was “the withholding period has passed,” while that for preventing contamination with residual needle fragments was “the needle was not included.” No deviations from critical limits were observed, indicating the pigs were safe. In addition, the numerical goal of pigs born alive per litter …


A Preliminary Study Of Tracheal Morphology In Mixed-Breed Dog Cadavers: Anatomical Variation Of Tracheal Cartilaginous Rings, Naparee Srisowanna, Sirin Theerawatanasirikul, Sukumal Prukudom, Niyada Lansubsakul, Montri Pattarapanawan, Maneenooch Khiao-In Aug 2024

A Preliminary Study Of Tracheal Morphology In Mixed-Breed Dog Cadavers: Anatomical Variation Of Tracheal Cartilaginous Rings, Naparee Srisowanna, Sirin Theerawatanasirikul, Sukumal Prukudom, Niyada Lansubsakul, Montri Pattarapanawan, Maneenooch Khiao-In

The Thai Journal of Veterinary Medicine

Anatomical abnormalities of the trachea leading to respiratory distress. Few reports describe the morphological characterization of the dog’s trachea, particularly in the tracheal cartilaginous rings. In this study, twelve tracheas from mixed-breed dog cadavers were obtained to investigate their features across three anatomical tracheal regions: cervical, thoracic inlet, and intrathoracic trachea. The average length of the trachea was 18.92 ± 0.67 cm. Each trachea contained a different number of tracheal rings, ranging from 32 to 41 rings (37.42 ± 0.81 rings). A significant reduction in both outer transverse diameter (OTD) and outer vertical diameter (OVD) was observed from the cervical …


First Serological Evidence Of Coxiella Burnetii And Mycobacterium Avium Subspecies Paratuberculosis (Map) Among Deer In An Institutional Farm In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Shivnraj Sundar Universiti Putra Malaysia, Nur Hanis Nadhirah Yosryhan, Yonis Ahmed Ahmed Jimale Dr, Eric Lim Teik Chung Dr, Mohd Azmi Mohd Lila Prof Aug 2024

First Serological Evidence Of Coxiella Burnetii And Mycobacterium Avium Subspecies Paratuberculosis (Map) Among Deer In An Institutional Farm In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Shivnraj Sundar Universiti Putra Malaysia, Nur Hanis Nadhirah Yosryhan, Yonis Ahmed Ahmed Jimale Dr, Eric Lim Teik Chung Dr, Mohd Azmi Mohd Lila Prof

The Thai Journal of Veterinary Medicine

Q fever is a zoonotic disease caused by Coxiella burnetii, which is associated with reproductive disorders such as abortion and reproductive problems in ruminants, while paratuberculosis is an underdiagnosed disease caused by Mycobacterium avium paratuberculosis (MAP) which causes chronic wasting, low fertility, and mortality in ruminants. Although both C. burnetii and MAP infections have been reported in domestic ruminants worldwide, there is no data on their seroprevalence status among deer in Selangor, Malaysia. This study investigated the seroprevalence status of C. burnetii and MAP among deer in Malaysia. Ninety-two blood sera collected from an institutional deer farm were screened …


Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan Aug 2024

Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan

The Thai Journal of Veterinary Medicine

The presence of extended-spectrum beta-lactamase and colistin resistance E. coli in food-producing animals is a public health concern. This study was conducted using a rigorous methodology to survey the prevalence of ESBL-producing and colistin-resistant E. coli from pig farms in the central part of Thailand. A total of 519 samples were collected from fecal, feed, and waste in 53 farms located in central Thailand. All samples were inoculated onto selective agar, and species identification was performed using biochemical assays and MAlDI-TOF MS. Antimicrobial susceptibility was determined by broth microdilution, and ESBL production was confirmed by the combination disc diffusion method. …


A Preliminary Study Of Lasia Spinosa Thw. Feed Supplementation Improving Semen Quality Of Subfertile Purebred Boars, Supitcha Kaewma, Junpen Suwimonteerabutr, Nutthee Am-In, Saritvich Panyaboriban, Mongkol Techakumphu, Theerawat Swangchan-Uthai Aug 2024

A Preliminary Study Of Lasia Spinosa Thw. Feed Supplementation Improving Semen Quality Of Subfertile Purebred Boars, Supitcha Kaewma, Junpen Suwimonteerabutr, Nutthee Am-In, Saritvich Panyaboriban, Mongkol Techakumphu, Theerawat Swangchan-Uthai

The Thai Journal of Veterinary Medicine

Poor semen quality is a significant issue among imported purebred boars in Thailand, affecting both semen quality itself and reducing productivity at AI stations. The present study investigated the effects of dietary supplementation with Lasia spinosa Thw. (LST) on improving boar semen quality. A group of boars experiencing poor semen quality were monitored, and three were selected due to their persistent low semen profiles for the supplementation experiment. Dried LST powder was prepared in small bags at a dosage of 4 g per 100 kg body weight and added to the regular feed for daily oral administration over an 8-week …


Quantitative Evaluation Of Different Fixatives For Masson Trichrome Staining In Canine Intestinal Tissue: An Image Analysis-Based Comparative Study, Siriluck Juntausa, Kongkiat Srisuwatanasagul, Kittipot Kongsonthana, Punnarat Vibulchan, Sayamon Srisuwatanasagul Aug 2024

Quantitative Evaluation Of Different Fixatives For Masson Trichrome Staining In Canine Intestinal Tissue: An Image Analysis-Based Comparative Study, Siriluck Juntausa, Kongkiat Srisuwatanasagul, Kittipot Kongsonthana, Punnarat Vibulchan, Sayamon Srisuwatanasagul

The Thai Journal of Veterinary Medicine

Neutral buffer formalin (NBF) has been widely used as a fixative for histological staining for ages. However, for some special staining, such as Masson trichrome staining, it has been proposed that the suitable fixative was Bouin’s solution. The purpose of the present study was to compare the different fixatives and post-fixative protocols on the quality of Masson trichrome staining. The canine intestines (n = 10), which were the duodenum and colon, were used as the candidate organs since they comprised of various tissue components. The fixatives and staining protocols were divided into four groups, which were Groups 1 and 2; …


Effect Of Heat Stress And Feed Type On Trimethylamine Concentration And Fishy Odor Of Duck Eggs, Pachara Pearodwong, Winai Kaewlamun, Sarawanee Khunmanee, Thuwanon Boonkerd, Thananon Junsuri, Kajohn Nitiwararak, Pantira Hempattarasuwan, Pattrarat Chanchaithong Aug 2024

Effect Of Heat Stress And Feed Type On Trimethylamine Concentration And Fishy Odor Of Duck Eggs, Pachara Pearodwong, Winai Kaewlamun, Sarawanee Khunmanee, Thuwanon Boonkerd, Thananon Junsuri, Kajohn Nitiwararak, Pantira Hempattarasuwan, Pattrarat Chanchaithong

The Thai Journal of Veterinary Medicine

We investigated the effect of heat stress and feed type on the trimethylamine (TMA) concentration and the fishy odor of duck eggs (Study 1) and the relationship between the TMA level in serum and egg content (Study 2). In Study 1, pooled egg samples (n = 75) were divided by feed type (CP12, n = 24; CP18, n = 27; CP20, n = 24) and heat stress (no, n = 49; moderate, n = 15; severe, n = 11). The collected samples were obtained for TMA analysis and sensory testing. In Study 2, a total of 15 egg and 15 …


Intraluminal Tracheal Stenting In Dog With Tracheal Collapse : A Case Report, Ratiporn Tantisak, Siriluck Luckanahasaporn, Ardong Atiptamvaree, Tamolwan Anyamaneecharoen, Kamonchanok Lamsaman, Suphat Soeratanapant, Poondej Charunmethee Aug 2024

Intraluminal Tracheal Stenting In Dog With Tracheal Collapse : A Case Report, Ratiporn Tantisak, Siriluck Luckanahasaporn, Ardong Atiptamvaree, Tamolwan Anyamaneecharoen, Kamonchanok Lamsaman, Suphat Soeratanapant, Poondej Charunmethee

The Thai Journal of Veterinary Medicine

A 12-year-old female neutered dog was referring to the pet hospital for the chief complaint of syncopic episodes 3 times/day. The dog had history of severe goose-honking non-productive cough and tachypnea. The dog got worsen despites medical management was consist of antibiotic, mucolytic, bronchodilator, glucocorticoids and nebulization. The dog was not response to medication then develop to apnea due to obstructive breathing. The radiographs diagnosis from private clinic was redundant trachea with narrowing intrathoracic trachea. The surgical treatment was considered. The tracheobronchoscopy was confirmed Tracheal collapse at mid cervical to cranial thoracic part. Stenting was a treatment options in life-threatening …


Application Of Metoclopramide As Gastroprokinetic In Koi Carp (Cyprinus Carpio) With Positive Buoyancy Disorders, Pornpawit Tanpichai, Thanaboon Katechaimongkol, Patharapol Piamsomboon, Thanida Haetrakul Aug 2024

Application Of Metoclopramide As Gastroprokinetic In Koi Carp (Cyprinus Carpio) With Positive Buoyancy Disorders, Pornpawit Tanpichai, Thanaboon Katechaimongkol, Patharapol Piamsomboon, Thanida Haetrakul

The Thai Journal of Veterinary Medicine

A two-year-old Koi carp or fancy carp (Cyprinus carpio) with a symptom of recurrent positive buoyancy was admitted to the Veterinary Medical Aquatic Animal Research Center of Excellence (VMARCE), Faculty of Veterinary Science, Chulalongkorn University. Radiographic examination with positive contrast media revealed gas accumulation in the gastrointestinal tract without obstruction; subsequently, idiopathic intestinal pseudo-obstruction was suspected. The anti-flatulence drug simethicone was administered, but the fish did not respond to the treatment. Therefore, metoclopramide, as the medical trial at a dosage of 0.5 mg/kg, was intramuscularly injected once daily for four consecutive days. The fish convalesced with neutral buoyancy …


Cardiac Biomarkers For Monitoring Canine Heartworm Disease: A Comparative Study Of N-Terminal Pro B Type Natriuretic Peptide And Cardiac Troponin I Levels, Sariya Asawakarn, Kittipong Tachampa, Piyanan Taweethavonsawat Aug 2024

Cardiac Biomarkers For Monitoring Canine Heartworm Disease: A Comparative Study Of N-Terminal Pro B Type Natriuretic Peptide And Cardiac Troponin I Levels, Sariya Asawakarn, Kittipong Tachampa, Piyanan Taweethavonsawat

The Thai Journal of Veterinary Medicine

Canine heartworm disease is a global mosquito-borne parasitic illness that affects dogs. The main species of filarids responsible for this disease is Dirofilaria immitis. This article specifically explores the use of cardiac biomarkers, such as N-terminal pro B type natriuretic peptide (NT-proBNP) and cardiac troponin I (cTnI), for diagnosing and monitoring canine heartworm disease. These biomarkers can indicate myocardial injury and heart failure, particularly in chronic infections. The study compares the levels of NT-proBNP and cTnI in dogs with heartworm disease, both clinically diagnosed cases and those without symptoms. The results reveal that cTnI is elevated in both cases, …


Quantitative Assessment Of Preoperative And Postoperative Brachycephalic Airway Obstruction Syndrome Surgery Using Clinical Grading Questionnaires And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Pasakorn Brikshavana, Sumit Durongphongtorn Aug 2024

Quantitative Assessment Of Preoperative And Postoperative Brachycephalic Airway Obstruction Syndrome Surgery Using Clinical Grading Questionnaires And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Pasakorn Brikshavana, Sumit Durongphongtorn

The Thai Journal of Veterinary Medicine

Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds, leading to clinical signs and physical capacity impairment. The degree of stenotic nares was reported to be related to BAOS severity in French bulldogs. This study aimed to compare clinical signs and physical capacity in French bulldogs before and after BAOS surgery using modified Roedler questionnaires and a 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study (non-BAOS control (n=7), non-severe stenotic nares BAOS dogs (n=8), and severe stenotic nares BAOS dogs (n=12). BAOS surgery included staphylectomy using bipolar …


Conventional Vs Three-Dimensional Computed Tomography For Measurement Of Tibial Tuberosity To Trochlear Groove Distance In Small Breed Dogs With And Without Medial Patellar Luxation, Radka S. Garnoeva Aug 2024

Conventional Vs Three-Dimensional Computed Tomography For Measurement Of Tibial Tuberosity To Trochlear Groove Distance In Small Breed Dogs With And Without Medial Patellar Luxation, Radka S. Garnoeva

The Thai Journal of Veterinary Medicine

The operative technique for medial patellar luxation (MPL) is dependent on the degree of accompanying bone deformities. The most frequent abnormalities: the shallow trochlear groove and tibial tuberosity medialisation are corrected surgically with trochleoplasty and tibial tuberosity transposition, respectively. The aim of the present study was to determine the distance between the tibial tuberosity and the trochlear groove (TT-TG) on two-dimensional axial computed tomography (CT) scans and three-dimensional multiplanar reconstruction images from 21 stifles from healthy dogs and 15 stifles from dogs with grade 2 MPL and to compare the performance of both methods. Two axial CT scans of the …


Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand, Thanh Che Nguyen, Chairani Ridha Maghfira, Nathaya Young, Kanokwan Samatiwat, Chaiyan Kaewpaluk, Apisak Laothanakit, Pawarisa Mungprasittichai, Roongroje Thanawongnuwech, Yaowalak Panyasing Aug 2024

Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand, Thanh Che Nguyen, Chairani Ridha Maghfira, Nathaya Young, Kanokwan Samatiwat, Chaiyan Kaewpaluk, Apisak Laothanakit, Pawarisa Mungprasittichai, Roongroje Thanawongnuwech, Yaowalak Panyasing

The Thai Journal of Veterinary Medicine

Gastrointestinal infectious diseases caused by bacteria have been a major problem in the swine industry, including Thailand. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis – an obligate intracellular bacterium. PPE has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied to the seroprevalence of pathogen-antibodies and the timing of L. intracellularis infection when introduced to the herd. This present study showed the current serological status and the seroprevalence of antibodies against L. intracellularis in large-scale Thai swine …


Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış Aug 2024

Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış

The Thai Journal of Veterinary Medicine

Mammary tumors are the most common neoplasm in dogs, with a high mortality rate. The development of canine mammary tumors (CMT) is multifactorial, but different incidence rates between breeds suggest the effects of genetic risk factors. Many CMT-associated candidate genes were reported, mainly involved in cell cycle control. This study aims to investigate p27 tumor suppressor gene polymorphisms in dogs with mammary tumors and analyze the association between variations and CMTs. For this purpose, case and control groups were formed from 22 dogs diagnosed with malignant mammary tumors and 10 dogs with healthy mammary glands. The whole canine p27 gene …


Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti Aug 2024

Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti

The Thai Journal of Veterinary Medicine

Flavonol quercetin (Q), found in vegetables and fruits, demonstrates the most potent anti-inflammatory effect. The preventive effect and cellular mechanisms of Q (Q1, Q10, and Q100 µM) on IL-1β induced leaky gut via the signaling molecules of inflammatory pathway, NF-κB p65-iκB/or p38/ERK1/2/MLCK/phospho(p)-MLC were investigated in Caco-2 cells. The cytotoxic effect of Q was initially determined by MTT assay. Transepithelial electrical resistance (TER) and apical-to-basolateral fluorescein isothiocyanate-labeled dextran (FD-4) flux were used to determine paracellular permeability. The protein expression of signaling molecules was examined by Western blot analysis, and their target MLCK mRNA expression was determined using real-time PCR. All Q …


Impacts Of Species, Gender Disparities, And Covid-19 Lockdowns On Population Control: Insights From 337,590 Dogs And Cats In Bangkok Metropolitan Administration (Bma), Thailand (2009-2023), Shanaporn Leelakajornkit, Natchanon Dumniem, Permsak Wataganara, Siwa Maison, Suppawiwat Ponglowhapan Aug 2024

Impacts Of Species, Gender Disparities, And Covid-19 Lockdowns On Population Control: Insights From 337,590 Dogs And Cats In Bangkok Metropolitan Administration (Bma), Thailand (2009-2023), Shanaporn Leelakajornkit, Natchanon Dumniem, Permsak Wataganara, Siwa Maison, Suppawiwat Ponglowhapan

The Thai Journal of Veterinary Medicine

Surgical sterilization stands as a key solution to address the overpopulation of dogs and cats, with numerous influencing factors impacting the success of population control efforts, including species, gender, and financial conditions. The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or COVID-19, in 2020 has significantly altered human life and impacted pet animals. Lockdown restrictions were implemented in various countries, including Thailand, affecting the cumulative data of neutered animals, which was subsequently analyzed in this study. There were 337,590 dogs and cats from 2009 to 2023, and the data were systematically categorized by years, species, and gender. …


Declined Lawsonia Intracellularis In Feces By Phytogenic Feed Additive Supplementation In Fattening Pigs In Different Herds System, Morakot Nuntapaitoon Aug 2024

Declined Lawsonia Intracellularis In Feces By Phytogenic Feed Additive Supplementation In Fattening Pigs In Different Herds System, Morakot Nuntapaitoon

The Thai Journal of Veterinary Medicine

The objective of the present study was to determine the efficacy of phytogenic feed additives on the number of L. intracellularis in feces and incidence of diarrhea in fattening pigs in different herds system. The present study was carried out in two commercial swine farms located in the northern (Herd A) and western (Herd B) parts of Thailand. In total, 80 fattening pigs (12-week-olds) were randomly allocated into two groups, including the Control group (n=40) and the Treatment group (n=40). The pigs in each group were fed with a conventional diet (Control) and the same diet supplemented with 2 kg/ton …


Clinical Response To Oclacitinib In Canine Cutaneous Epitheliotropic T-Cell Lymphoma: A Case Reports, Takuma Imai Aug 2024

Clinical Response To Oclacitinib In Canine Cutaneous Epitheliotropic T-Cell Lymphoma: A Case Reports, Takuma Imai

The Thai Journal of Veterinary Medicine

The chemotherapy for canine cutaneous epitheliotropic T cell lymphoma (CETL) might cause unpleasant side effects or fail to produce positive results. We attempted a different approach with a more tolerable treatment option. We administered a high dose of oclacitinib (1.6 mg/kg/twice daily) to a patient diagnosed with CETL through histopathological examination and immunocytochemical staining. The patient had previously shown a poor response to chemotherapy and severe side effects to steroids. Within one month of starting oclacitinib treatment, we observed significant improvement in clinical symptoms, followed by improved appetite, increased activity, and weight gain after 2.5 months. However, subsequently, the patient's …


A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur Aug 2024

A Case Of Conjunctivitis Due To Mdr-Mr Staphylococcus Pseudintermedius In A Dog, Hazel Tamakan Yesi̇lovali, Hali̇t Sukur

The Thai Journal of Veterinary Medicine

A young male Dachshund dog who came to the animal hospital was diagnosed with conjunctivitis on clinical examination. Methicillin-resistant Staphylococcus pseudintermedius was identified as a result of culture and bacteriological examination of the conjunctival swab, and then antimicrobial susceptibility test. Methicillin resistant S. pseudintermedius identified with the VITEK-II Compact device was also confirmed by molecular methods so that susceptible antibiotics were selected for the treatment of the patient and reported. In accordance with the antimicrobial susceptibility results, the patient was treated with an eye drop preparation containing neomycin. This is the first report of a canine conjunctivitis caused by multidrug-methicillin …


Computed Tomography And Magnetic Resonance Imaging Of Maxillofacial Rhabdomyosarcoma And Suspected Multiple Metastases Including The Epidural Space And Bone Marrow In A Dog, Sang-Kwon Lee, Kija Lee Aug 2024

Computed Tomography And Magnetic Resonance Imaging Of Maxillofacial Rhabdomyosarcoma And Suspected Multiple Metastases Including The Epidural Space And Bone Marrow In A Dog, Sang-Kwon Lee, Kija Lee

The Thai Journal of Veterinary Medicine

A 3-year-old mixed-breed dog presenting with a recurrent maxillofacial tumor was diagnosed with recurrent rhabdomyosarcoma. Computed tomography and magnetic resonance imaging revealed a left maxillofacial mass, left cervical lymph node enlargement, epidural mass dorsal to the 6th lumbar vertebra compressing the cauda equina, soft-tissue mass ventral to the 6th lumbar vertebra, multiple subcutaneous nodules and signal changes in the paravertebral muscles. The left maxilla, lumbar spine and ilium exhibited diffuse signal changes and contrast enhancement upon magnetic resonance imaging. This is, to our knowledge, the first reported case of bone marrow and epidural-space involvement of a canine rhabdomyosarcoma.


Detection Of Mycoplasma Synoviae Antibodies In Day-Old Chicks Using Rapid Serum Agglutination Assay And Enzyme-Linked Immunosorbent Assay, Kriengwich Limpavithayakul, Somsak Pakpinyo Aug 2024

Detection Of Mycoplasma Synoviae Antibodies In Day-Old Chicks Using Rapid Serum Agglutination Assay And Enzyme-Linked Immunosorbent Assay, Kriengwich Limpavithayakul, Somsak Pakpinyo

The Thai Journal of Veterinary Medicine

Mycoplasma synoviae antibodies detection using rapid serum agglutination (RSA) assay and enzyme-linked immunosorbent assay (ELISA) is considered as the suitable screening tool in chicken flocks. Both RSA assay and ELISA have performed an ability to detect an antibody formation during mycoplasma infection due to their sensitivity in detecting both IgM and IgY. However, the IgY is the major antibody isotype transferred from the hen to the newly hatched broiler chick via egg yolk. The amount of maternal IgY circulating in the chicks’ serum is approximately 30% of the IgY level present in the hen’s bloodstream. This study was conducted to …


High-Speed Dental Drill May Be An Acceptable Instrument Performing Canine Laminectomy, Ziyao Zhou Aug 2024

High-Speed Dental Drill May Be An Acceptable Instrument Performing Canine Laminectomy, Ziyao Zhou

The Thai Journal of Veterinary Medicine

Laminectomy is usually used in spinal cord dysfunction like intervertebral disc herniation. High-speed pneumatic surgical drill is the most common surgical instrument for canine laminectomy operation. However, since the finical problem, the professional instrument has a low installation rate in animal hospitals in developing country. In this study, we hypothesized that dental drill may replace pneumatic surgical drills in dorsal laminectomy and hemilaminectomy, observed if dorsal laminectomy and hemilaminectomy can been successfully operated by dental drill, and compared the canine dorsal laminectomy and hemilaminectomy. Operations were performed in L2-L3 of lumbar vertebra among 12 Chinese rural dogs (6 for dorsal …


Self-Replicating Rna For Cellular Reprogramming In Domestic Cat, Woranop Sukparangsi, Nattakorn Suban Na Ayuthaya, Ratchapon Prakongkaew, Santhita Karoon, Ampika Thongphakdee Aug 2024

Self-Replicating Rna For Cellular Reprogramming In Domestic Cat, Woranop Sukparangsi, Nattakorn Suban Na Ayuthaya, Ratchapon Prakongkaew, Santhita Karoon, Ampika Thongphakdee

The Thai Journal of Veterinary Medicine

Cat Induced Pluripotent Stem Cells have been previously established using viral vectors integrating into the host genome to deliver reprogramming factors. To aid wild felid conservation using stem cell technology, in this study we utilized non-integrative Venezuelan Equine Encephalitis-based self-replicating RNA (srRNA) to carry the reprogramming factors. This approach induced cat somatic cells towards pluripotency without genomic modification, supporting the future use of srRNA in wild felid cells. The srRNA expressing human OCT4, SOX2, KLF4, CMYC, and GLIS1 (OSKMG) could induce the formation of cat iPSC colonies after 4 rounds of transfections. Different species-specific Leukemia Inhibitory Factor (LIF) could capture …


Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid, Jaroon Wandee, Piyarat Srinontong, Worapol Aengwanich Aug 2024

Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid, Jaroon Wandee, Piyarat Srinontong, Worapol Aengwanich

The Thai Journal of Veterinary Medicine

The study aimed to investigate the effect of tannic acid (TA) on total antioxidant power (FRAP), malondialdehyde (MDA), hydrogen peroxide (H2O2), nitric oxide (NO), and the viability of broiler blood cells (BBC) exposed to high ambient temperatures. The BBC from a broiler chicken was exposed to temperatures ranging from 41.5°C to 46°C and incubated with TA at concentrations of 0 (the positive control group, TPCG), 5, 10, 15, and 20 μg/mL. Results showed that MDA, H2O2, and NO levels of the control group (TCG) which BBC was maintained at 41.5°C were significantly lower than TPCG. The viability of TPCG was …


Biomechanical Comparison Of Transosseous Four-Strand Tendon Suture Patterns For Repair Of Ruptured Distal Common Calcaneal Tendon: An Ex Vivo Study In Dogs, Tae-Hwan Ahn, Min-Yeong Lee, Jaekyoung Lee, Hwi-Yool Kim Aug 2024

Biomechanical Comparison Of Transosseous Four-Strand Tendon Suture Patterns For Repair Of Ruptured Distal Common Calcaneal Tendon: An Ex Vivo Study In Dogs, Tae-Hwan Ahn, Min-Yeong Lee, Jaekyoung Lee, Hwi-Yool Kim

The Thai Journal of Veterinary Medicine

There is no golden standard method of suturing for distal common calcaneal tendon rupture in dogs. Thus this study aimed to evaluate and compare the mechanical characteristics of three distinct transosseous suture (TOS) repair techniques, which were specifically developed to treat ruptured distal common calcaneal tendons and to identify golden standard suturing method. The study was performed using 30 hind limbs obtained from 15 canine cadavers with weights ranging between 7.9 kg and 11.4 kg. The repair methods using modified Becker suture (group 1), modified Krackow suture (group 2), and modified double-Tsuge suture (group 3) were randomly assigned. Each group …


The Molecular Detection Of Hemotropic Mycoplasma Species In Dogs, Hafize Tuğba Yüksel Dolgun, Şükrü Kirkan Aug 2024

The Molecular Detection Of Hemotropic Mycoplasma Species In Dogs, Hafize Tuğba Yüksel Dolgun, Şükrü Kirkan

The Thai Journal of Veterinary Medicine

Hemotropic mycoplasmas are uncultured bacteria that cause different degrees of hemolytic anemia in infected hosts. Molecular approaches are used to diagnose hemotropic mycoplasmas since they are difficult to identify using conventional methods. The aim of this study was to use molecular approaches to determine hemotropic mycoplasmas in dogs. Blood samples were collected in tubes (with EDTA) from 100 dogs. After DNA extraction, 100 DNA samples were investigated by PCR. Hemotropic mycoplasma was detected in 12 (12%) samples and positive samples were subjected to Sanger sequencing. Mycoplasma haemocanis was detected in 6 (50.0%) of the samples, Candidatus Mycoplasma hematoparvum in 5 …


Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong Aug 2024

Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the effects of hydrogel containing nanosilver particles on a second-degree burn wound in a porcine model. The second degree burn wound was performed using 1 cm in diameter, on 100 °C heated cylindrical brass bar applied to the dorsum of pig skin. Burn wounds were randomly divided into three groups of wound dressings (WDs) including commercial polyurethane hydrogel (Cutinova Hydro®, Gr. A), in-house hydrogel (Gr. B) and in-house nanosilver hydrogel (Gr. C). The wound contraction rate and hyperemic zone were measured and investigated at 3, 7, 14 and 21 days after dressing. …