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Resilience And Associated Factors Of Family Caregivers Of The Terminal Illness Patients At Cheewabhibaln Center, King Chulalongkorn Memorial Hospital, Kornsiri Pikulthong, Peeraphon Lueboonthavatchai Jul 2021

Resilience And Associated Factors Of Family Caregivers Of The Terminal Illness Patients At Cheewabhibaln Center, King Chulalongkorn Memorial Hospital, Kornsiri Pikulthong, Peeraphon Lueboonthavatchai

Chulalongkorn Medical Journal

Background: Family caregivers play an important role to provide support and care for terminal illness patients by spending their time responding to the patients’ needs. Receiving this role can cause them to be stressful and anxious. Therefore, it is essential for them to have resilience so that they are able to overcome potentially all burdens. Objectives: The present study aimed to examine resilience and associated factors of family caregivers of the terminal illness patients at Cheewabhibaln Center, King Chulalongkorn Memorial Hospital. Methods: This descriptive study was conducted in 150 family caregivers. The sample group was recruited through the purposive sampling …


Quantitative Measurement In Knee Joint Effusion: Correlation Between Plain Radiographs And Mri, Chindarat Ratanakornphan, Teeraphorn Boonswang Jul 2021

Quantitative Measurement In Knee Joint Effusion: Correlation Between Plain Radiographs And Mri, Chindarat Ratanakornphan, Teeraphorn Boonswang

Chulalongkorn Medical Journal

Background: Knee joint effusion is a common manifestation of synovial disease resulting from traumatic injury, infection, inflammation and degenerative disease. Plain radiographs and magnetic resonance imaging (MRI) play an important role in diagnosing knee effusion. However, there is scant data demonstrating the correlation of the two studies. Objective: The purposes of our study were to investigate the correlation of knee effusion between thickness of radiodense area within the suprapatellar pouch on lateral radiographs and volume measurement on MRI studies, and to assess the distribution of effusion in various compartments. Methods: Quantitative measurement of effusion volume on sagittal fat-saturated T2 weighted …


Test Takers’ Perceptions Of Design And Implementation Of An Online Language Testing System At A Thai University During The Covid-19 Pandemic, Kornwipa Poonpon Jul 2021

Test Takers’ Perceptions Of Design And Implementation Of An Online Language Testing System At A Thai University During The Covid-19 Pandemic, Kornwipa Poonpon

PASAA

The COVID-19 pandemic caused challenges in language testing for many universities and institutions. The present study aimed to address some challenges and restrictions by designing and implementing an online testing system for a high-stakes proficiency test at a Thai university during the COVID-19 pandemic and exploring test takers’ perceptions of the design and its implementation. The online language testing system, i.e., test interface, test delivery mode, and proctoring system, were designed and implemented. The online test interface was developed using Fulcher’s (2003) computer-based language test development framework. The design and implementation of the synchronous at-home test delivery mode and the …


Correlation Among Pain Catastrophizing, Depressive Symptom, And Activity Of Daily Living In Older Thai Buddhist Monks With Knee Osteoarthritis, Thanakrit Jeamjitvibool, Suparb Aree-Ue, Phichpraorn Youngcharoen, Viroj Kawinwonggowit Jul 2021

Correlation Among Pain Catastrophizing, Depressive Symptom, And Activity Of Daily Living In Older Thai Buddhist Monks With Knee Osteoarthritis, Thanakrit Jeamjitvibool, Suparb Aree-Ue, Phichpraorn Youngcharoen, Viroj Kawinwonggowit

Chulalongkorn Medical Journal

Background: Older Thai Buddhist monks are usually accustomed to a unique lifestyle that potentially relates to knee osteoarthritis (OA) such as long-standing kneeling on the hard surface. Understanding the association among pain catastrophizing, depressive symptoms, and activity of daily living (ADL) in this population with knee OA may help reduce the psychosocial problems and promote their sacred activities. Objective: To determine the relationship between pain catastrophizing, depressive symptoms, and ADL in older Thai Buddhist monks with knee OA. Methods: In all, 105 older monks with knee OA were recruited in this study. Data were collected by using a set of …


Predictive Validity Of The Cu-Tep As An Indicator Of Students’ Academic Achievement In English, Chalatip Charnchairerk Jul 2021

Predictive Validity Of The Cu-Tep As An Indicator Of Students’ Academic Achievement In English, Chalatip Charnchairerk

PASAA

The Chulalongkorn University Test of English Proficiency (CU-TEP) is an institutional standardized English proficiency test designed to measure students’ ability to use English for academic purposes. It is required for admission to graduate programs and undergraduate English programs at Chulalongkorn University and some other educational institutions. This study examined how accurate CU-TEP was as a predictor of the academic achievement of a total of 520 first-year Chulalongkorn University students as measured by three compulsory English course results and two English writing task scores. The participants were selected from a cohort of 5,564 freshmen taking CU-TEP in 2014, using the systematic …


Flood Hazard Mapping And Assessment Of Precipitation Monitoring System Using Gis-Based Morphometric Analysis And Trmm Data: A Case Study Of The Wadi Qena Watershed, Egypt, Wael M. Elsadek, Wael E. Mahmod, Mona G. Ibrahim Jul 2021

Flood Hazard Mapping And Assessment Of Precipitation Monitoring System Using Gis-Based Morphometric Analysis And Trmm Data: A Case Study Of The Wadi Qena Watershed, Egypt, Wael M. Elsadek, Wael E. Mahmod, Mona G. Ibrahim

Applied Environmental Research

Wadi Qena is one of the Nile Valley areas particularly at risk of severe flash flooding, located in Egypt. The study aims to verify TRMM rainfall data (TRMM 3B42), using eight stations across Egypt as well as relies on morphometric analysis to generate a flood risk map based on the ranking method. Three process could be recognized through the study, calibration, correction and verification processes. The results discuss the match daily rainfall trends of TRMM and observed data, producing a correction equation for TRMM data with root mean square error (RMSE) value of 0.837 mm d-1 and R2= 0.238 (calibration …


Application Of Gis And Multi-Criteria Statistical Techniques In Assessing Water Quality In The Coastal Province Of Vietnamese Mekong Delta, Giao Thanh Nguyen, Thi Hong Nhien Huynh Jul 2021

Application Of Gis And Multi-Criteria Statistical Techniques In Assessing Water Quality In The Coastal Province Of Vietnamese Mekong Delta, Giao Thanh Nguyen, Thi Hong Nhien Huynh

Applied Environmental Research

The study was conducted to evaluate the quality and spatial distribution of surface water quality in Soc Trang, a coastal province using Geographic information system (GIS) and multivariate statistical analysis. Water monitoring data was collected from 19 sampling locations with 19 parameters were analyzed from February 2019 to August 2020. The results indicated that water quality was contaminated with organic matters, nutrients, coliforms and salinity. Water quality index (WQI=22–73) indicated that water quality was from poor to medium level. Cluster analysis (CA) classified 19 monitoring sites into 7 groups and 19 months into 3 seasons including rainy season, rainy season-early …


Adsorption Of Methylene Blue By Low-Cost Biochar Derived From Elephant Dung, Yanasinee Suma, Nittaya Pasukphun, Numfon Eaktasang Jul 2021

Adsorption Of Methylene Blue By Low-Cost Biochar Derived From Elephant Dung, Yanasinee Suma, Nittaya Pasukphun, Numfon Eaktasang

Applied Environmental Research

Elephant dung biochar (ED350) prepared by controlled heating at 350 ºC was used to adsorb methylene blue (MB) in an aqueous solution. The effects of adsorption time, pH, adsorbent dosage, and initial MB concentration were examined. Kinetic, isotherm, and thermo-dynamic models were then further analyzed to determine the adsorption. The results show that ED350 was found to be efficient within 180 min. The optimum pH of MB adsorption was 11. The Langmuir isotherm model was found to be the most suitable fit for the adsorption equilibrium data, with ED350 having a homogeneous surface. The calculated equilibrium parameter (RL) …


A Workforce And Truck Allocation Model In A Solid Waste Management System: A Case From Nigeria, Desmond E. Ighravwe, Sunday A. Oke Jul 2021

A Workforce And Truck Allocation Model In A Solid Waste Management System: A Case From Nigeria, Desmond E. Ighravwe, Sunday A. Oke

Applied Environmental Research

No abstract provided.


Spatiotemporal Flood Risk Determination And Management For A Large River Basin, Nuanchan Singkran Jul 2021

Spatiotemporal Flood Risk Determination And Management For A Large River Basin, Nuanchan Singkran

Applied Environmental Research

No abstract provided.


Synergistic Effect Of Waste Feedstock Mixture And Torrefaction Pre-Treatment On Some Briquette Characteristics Of Cornhusk And Sawdust Wastes, Mufutau A. Waheed, Opeyemi A. Akogun Jul 2021

Synergistic Effect Of Waste Feedstock Mixture And Torrefaction Pre-Treatment On Some Briquette Characteristics Of Cornhusk And Sawdust Wastes, Mufutau A. Waheed, Opeyemi A. Akogun

Applied Environmental Research

Agricultural waste management through energy recovery is one of the critical options that could drive the clean energy production industry and properly replace the use of coal in Nigeria if done sustainably. The objective of this work therefore is to study the synergetic effect of waste feedstock mixture and torrefaction pre-treatment on some physical and performance characteristics of briquettes from cornhusk (CH) and sawdust (SD) wastes. In this work, CH and SD wastes were processed raw and torrefied at 200 to 300 °C and were mixed in the ratios between 90/10 and 10/90 for briquette production using D-optimal crossed design. …


Personality Traits And Smoking Behavior In Patients At The Check – Up Clinic, King Chulalongkorn Memorial Hospital, Pasharapa Jatuporn, Chotiman Chinvararak Jul 2021

Personality Traits And Smoking Behavior In Patients At The Check – Up Clinic, King Chulalongkorn Memorial Hospital, Pasharapa Jatuporn, Chotiman Chinvararak

Chulalongkorn Medical Journal

Background: Cigarettes are caused of health issues and premature death of the smoking population, including the cause of other problems, such as other types of illegal drugs usage, which is the sources of crimes. Thus, recognition of smoking behavior will become increasingly important. Personality traits might associate with cigarette consumption. However, there are still restricted numbers of studies with conflicting data. Objectives: To study factors association between personality and smoking behaviors in patients at the Check – up Clinic, King Chulalongkorn Memorial, Hospital. Methods: This study is a cross-sectional descriptive study. Data collection was performed using a personal information questionnaire, …


Effect Of An Information And Communication Technology Program On The Anxiety Of Coronary Artery Bypass Graft Patients’ Caregivers In Intensive Care Unit, Jirakit Duangjuk, Usavadee Asdornwised, Kessiri Wongkongkam, Teerapong Tocharoenchok Jul 2021

Effect Of An Information And Communication Technology Program On The Anxiety Of Coronary Artery Bypass Graft Patients’ Caregivers In Intensive Care Unit, Jirakit Duangjuk, Usavadee Asdornwised, Kessiri Wongkongkam, Teerapong Tocharoenchok

Chulalongkorn Medical Journal

Background: The caregivers of coronary artery bypass graft (CABG) patients are typically very anxious while the patients are treated in the intensive care unit (ICU). Objective: To study the effects of an information and communication technology program on the anxiety level of CABG patients’caregivers during patients’ admission to the ICU. Methods: This is a quasi-experimental research. The sample group is caregivers of CABG patients. Thirty-four caregivers in the control group that received only routine nursing care while 34 caregivers in the experimental group received the information and communication technology program and routine nursing care. Data were collected using the Thai …


Working Happiness And Employee Engagement At Publishing Company., Ltd, Yosaporn Punjamawat, Paul Thisayakorn Jul 2021

Working Happiness And Employee Engagement At Publishing Company., Ltd, Yosaporn Punjamawat, Paul Thisayakorn

Chulalongkorn Medical Journal

Background: The purposes of this research were to study work happiness, employee engagement, and related factors in employees at a medium Publishing Co.,Ltd. Objectives: To study work happiness, employee engagement and related factors that contribute to work happiness of permanent employees at a medium Publishing Co., Ltd. in Bangkok. Methods: Data were collected from 124 employees a medium Publishing Co., Ltd. The questionnaire included general information, work happiness questionnaire, Corporate Engagement Questionnaire and the Patient Health Questionnaire - 9 (PHQ-9). The data were analyzed by Descriptive Statistics, unpaired t - test, One-way ANOVA, Spearman’s correlation co-efficiency and multiple liner regression. …


Effect Of Prewarmimg On Body Temperature And Shivering In Total Knee Arthroplasty Patients After Surgery, Ratchanee Sukanthachalathon, Usavadee Asdornwised, Orapan Thosingha, Phongthara Vichitvejpaisal Jul 2021

Effect Of Prewarmimg On Body Temperature And Shivering In Total Knee Arthroplasty Patients After Surgery, Ratchanee Sukanthachalathon, Usavadee Asdornwised, Orapan Thosingha, Phongthara Vichitvejpaisal

Chulalongkorn Medical Journal

Background: Inadvertent hypothermia and shivering is recognized risk in surgery. Core temperature in patients warmed with forced air warming remains poorly characterized. The incidence in patients with total knee arthroplasty (TKA) leading to intraoperative hypothermia 43.9%. Objectives: This quasi-experimental research study was conducted to compare body temperature and shivering after surgery in patients with total knee arthroplasty who received prewarming and those who received routine nursing care. Methods: Thirty-first patients were randomly assigned for experimental group and Thirty-first patients for control group. Data were collected by the following tools and methods, namely: patient demographic and clinical characteristic records, tympanic membrane …


Comparing Cytological Adequacy Between Conventional Smear And Liquid-Based Cytology In Ultrasound-Guided Fine Needle Aspiration Of Thyroid Nodules: A Prospective Study, Nattachai Kruajak, Pawanrat Kranokpiraksa, Tikamporn Jitpasutham Jul 2021

Comparing Cytological Adequacy Between Conventional Smear And Liquid-Based Cytology In Ultrasound-Guided Fine Needle Aspiration Of Thyroid Nodules: A Prospective Study, Nattachai Kruajak, Pawanrat Kranokpiraksa, Tikamporn Jitpasutham

Chulalongkorn Medical Journal

Background: The major problem of conventional smear for ultrasound-guided fine needle aspiration (FNA) of thyroid nodules is the sample inadequacy. Liquid-based cytology (LBC) is claimed to perform better than the conventional smear (CS) in reducing sample inadequacy. However, the evidence of better adequacy in LBC is unclear. Objective: This study is the first prospective study aiming to compare sample adequacy in CS and LBC using ThinPrep system with adequacy criteria based on the 2017 Bethesda System for Reporting Thyroid Cytopathology. Methods: One hundred and twenty thyroid nodules were prospectively recruited for ultrasound-guided FNA from March to September 2019. The sample …


Anxiety In Caregivers Of Palliative Care Patients, Worasiri Leongnarktongdee, Puchong Laurujisawat Jul 2021

Anxiety In Caregivers Of Palliative Care Patients, Worasiri Leongnarktongdee, Puchong Laurujisawat

Chulalongkorn Medical Journal

Background: Palliative care service undoubtedly aims at quality of life of both the patients and their family members. Caring for the ill can bring about mental burden to the caregivers; but this is understudied in Thai literature. Objective: This study aimed to explore the anxiety state and associated factors in caregivers of palliative patients, particularly in urban setting as Bangkok. Methods: We recruited 93 caregivers of inpatients consulted to the Palliative Care Unit of King Chulalongkorn Memorial Hospital. The instruments used include Edmonton Symptom Assessment System (ESAS) - Thai version and The State - Trait Anxiety Inventory (STAI Form). Multiple …


First Case Report Of High Volume Therapeutic Plasma Exchange As A Rescue Therapy In Dengue Hemorrhagic Fever With Acute Liver Failure, Surat Nakaviroj Jul 2021

First Case Report Of High Volume Therapeutic Plasma Exchange As A Rescue Therapy In Dengue Hemorrhagic Fever With Acute Liver Failure, Surat Nakaviroj

Chulalongkorn Medical Journal

Acute liver failure is a rare but life-threatening complication of severe dengue infection. Besides standard medical treatment, high volume therapeutic plasma exchange (HV-TPE) is a potential management strategy used to reverse hepatic encephalopathy and coagulopathy. HV-TPE, moreover, improves patient survival from acute liver failure due to paracetamol overdose, viral-induced hepatitis, and Wilson disease. The use of HV-TPE in acute liver failure with dengue hemorrhagic fever has never been reported. We, hereby, report a successful treatment with HV-TPE in acute liver failure with dengue hemorrhagic fever.


Prevalence And Associated Factors Of Anxiety And Depression Among Advanced Maternal Age Pregnancy, Kotchakorn Jeeratunyasakul, Chutima Roomruangwong Jul 2021

Prevalence And Associated Factors Of Anxiety And Depression Among Advanced Maternal Age Pregnancy, Kotchakorn Jeeratunyasakul, Chutima Roomruangwong

Chulalongkorn Medical Journal

Background: Advanced maternal age is associated with increased rate of various obstetrics risks. Nowadays, there is increasing prevalence of advanced maternal age in Thailand. However, there is still lack of study in anxiety and depression among this group of pregnant women. Objectives: To examine the prevalence of anxiety and depression as well as associated factors among women with advanced maternal age pregnancy Methods: We recruited 124 pregnant women age  35 years old who were in their third trimester (gestational age  28 weeks) of pregnancy. The subjects were asked to rate: The State-Trait Anxiety Inventory (STAI Form Y) - …


A Novel Custom-Made External Fixator For Treating Femur Fractures In Goats, Hsu-Hsun Lee, Ting-Sheng Lin, Lee-Shuan Lin, Cheng-Shu Chung, Shyh-Shyan Liu, Huan-Yu Hsu, Ching-Hou Ma, Jai-Wei Lee Jun 2021

A Novel Custom-Made External Fixator For Treating Femur Fractures In Goats, Hsu-Hsun Lee, Ting-Sheng Lin, Lee-Shuan Lin, Cheng-Shu Chung, Shyh-Shyan Liu, Huan-Yu Hsu, Ching-Hou Ma, Jai-Wei Lee

The Thai Journal of Veterinary Medicine

External fixation is commonly used to treat long bone fractures in goats. It provides good enough stabilization on fractures to improve healing, minimizes soft tissue injury and only causes mild obstruction of the blood supply around the wounds. However, the large size and heavy weight of traditional external fixators (TEFs) cause them leave something to be desired. In this study, the feasibility of a novel custom-made external fixator (CMEF) for treating femoral fractures in goats was evaluated. Three one-year-old Anglo-Nubian female goats were subjected to experimental femoral fractures to their left femur with a CMEF installed for fixation. To evaluate …


Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri Jun 2021

Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri

The Thai Journal of Veterinary Medicine

A 19-year-old polo pony presented with mild lameness alongside a lateral protuberance of the right tarsus. Radiographic findings revealed various defects, including an osteochondral lesion of the medial trochlear ridge of the talus, a sclerotic lesion of the tarsometatarsal joint, a radiolucent area on the distal border of the fourth tarsal bone and an enthesophyte at the proximolateral aspect of the metatarsal bone. Ultrasonography demonstrated the interesting finding of synovial sheath thickening of the lateral extensor tendon with synovial fluid accumulation. In addition, the presence of neutrophils and a granular mucinous background, according to cytological analysis of the fluid, indicated …


Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim Jun 2021

Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 6-year-old neutered male Siamese cat was presented with an acute onset of vomiting, anorexia and lethargy lasting for four days. An abdominal mass was palpated on physical examination. The cat showed severe anemia, elevated liver enzymes and hypoalbuminemia. Abdominal ultrasonography revealed a suspected hepatic mass without blood flow within the affected region. Computed tomography was performed to better visualize the abdominal cavity and blood flow. Prompt diagnosis of torsion of the left lateral liver lobe was made and immediate surgery for liver lobectomy was performed. Histopathological examination revealed no evidence of neoplasia. The cat remained clinically normal during the …


The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam Jun 2021

The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam

The Thai Journal of Veterinary Medicine

A three-year-old Saanen buck presented with a complaint of stranguria for 24 hours. A physical examination revealed that the perineal urethra was turgid, swollen, and tender, and the urinary bladder was distended. The goat was diagnosed with obstructive urolithiasis. A percutaneous tube cystotomy technique was performed to relieve progressive urinary bladder distension. Using a human suprapubic urinary bladder catheter system, the technique was performed twice due to the recurrence of the blocked urethra. Following drainage of the urine, all vital parameters returned to normal. After surgical cystotomy, an intraoperative observation revealed the urinary bladder was filled with white sandy urolith, …


Generalized Enamel Hypoplasia In A Dog: A Case Report, Issarin Sittijarusub, Panithi Sukho, Patchamon Supannawapat, Namphung Suemanotham Jun 2021

Generalized Enamel Hypoplasia In A Dog: A Case Report, Issarin Sittijarusub, Panithi Sukho, Patchamon Supannawapat, Namphung Suemanotham

The Thai Journal of Veterinary Medicine

A one-year-old, spayed female Welsh Corgi dog referred to the dental and oral clinic with chief complaint of apparent pitted, rough teeth surfaces, stained a tan to dark brown color. The dog was diagnosed with diffused enamel hypoplasia with no evidence of endodontic disease or malformation of tooth root. A restorative procedure with nanohybrid composite resin was performed. After treatment, the dog showed a good outcome with a smooth tooth surface, restorative material replaced to cover the previous defect and no evidence of pain or discomfort. Even though enamel hypoplasia is a rare, this condition remains an important problem that …


Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul Jun 2021

Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

Considering the impact of Salmonella in turtles and the role of turtles as a source of Salmonella outbreaks in humans, knowledge of Salmonella status in turtles is important. A cross-sectional study was conducted to investigate the prevalence, risk factors and antimicrobial resistance of Salmonella infections in turtles. Cloacal swabs were collected from 378 turtles in Turtle Villages located in Khon Kaen province of northeastern Thailand between January 2018 and April 2019. All samples were examined for Salmonella spp. isolation and identification by ISO 6597:2002. The overall prevalence of Salmonella in the turtles was 37.57% (142/378). Habitat, season and sex were …


Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn Jun 2021

Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objectives of this study were to determine the differences in the expression of heat shock protein, and steroidogenic and apoptosis genes by cumulus-oocyte complex (COC) of beef cattle in a tropical environment as affected by seasonal and day effect of temperature and relative humidity. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure gene expression in COC in different seasons including cool (Jan to Feb), summer (Mar to Apr) and rainy (May to Jul) seasons. Ambient temperature and relative humidity recorded on the day before oocyte collection were used to classify environment groups as: low temperature and low …


Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr Jun 2021

Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Porcine epidemic diarrhea virus (PEDV) is the causative agent of severe watery diarrhea in suckling piglets. PEDV outbreaks have been consistently reported in Europe, Asian countries and Thailand. The recombinant protein, especially the spike (S) protein, from field strain PEDV could be used to develop a sensitive diagnostic test or an effective vaccine. In this study, the recombinant S2 domain protein from the PEDV Thai isolate was expressed using the E. coli expression system. The full length S2 domain of the S gene of the PEDV G2b Thai isolate was amplified using a PCR technique and was cloned into the …


Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh Jun 2021

Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh

The Thai Journal of Veterinary Medicine

Sepsis is an important disease in both humans and animals. In animals, it is usually caused by septicaemia after infection and has not been well-studied. This article describes a retrospective analysis of sepsis cases in small and large ruminants submitted to the Post-Mortem Laboratory, Faculty of Veterinary Medicine, Universiti Putra Malaysia between the years 2007 and 2017. The incidence of sepsis was found to be at 40.5% in small ruminants and 38.6% in large ruminants. In both small and large ruminants, sepsis was mainly caused by bacterial infection, which was significantly (p<0.05) higher compared to parasitic and viral infections. Most cases of sepsis occur due to infections originating from the gastrointestinal tract and this is followed by the respiratory tract. Escherichia coli has been found to be the most common bacterial pathogen of sepsis in ruminants; significantly (p<0.05) higher compared to other bacterial pathogens like Klebsiella pneumoniae, Pasteurella sp., and Staphylococcus aureus. Sepsis involving E. coli has often resulted in pure isolation of the pathogen, thus being suggestive of a single infection. Underlying or concurrent infection is the most important risk factor leading to sepsis in ruminants, with significant difference (p<0.05) compared to other risk factors such as stress or malnutrition. Associations between the gastrointestinal system as the point of entry and sepsis caused by either E. coli or K. pneumoniae have been noted. This study provides an insight into a basic understanding of sepsis in ruminants.


Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen Jun 2021

Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …


Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha Jun 2021

Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha

The Thai Journal of Veterinary Medicine

Two hundred and fifty one-day old broiler chicks were used to evaluate the effect of black seeds and/or turmeric on performance and immune response to Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV) and Infectious Bursal Disease virus (IBDV) in broiler chickens of six weeks of age. The birds were divided into five groups (50 birds in each) with 5 replicates (10 birds each). Birds in the first group were fed a basal diet and served as a control. Birds of groups 2-5 were fed a basal diet mixed with black seed (20g/ kg basal diet), turmeric (20g/ kg basal …