Digital Commons Network^™

Synthesis, Docking Studies, Admet Prediction, And Antimicrobial Evaluation Of Coumarin-Oxadiazole Hybrids, Fabin Asmath Maziyuna, Abhishek Kumar, Pankaj Kumar

The Thai Journal of Pharmaceutical Sciences

The overuse and misuse of marketed antimicrobial agents developed drug resistance, which has become a significant problem worldwide. A series of new coumarinyl-oxadiazole Schiff base derivatives were designed and synthesized. These synthesized molecules synthesis of coumarinyl-oxadiazole schiff base derivatives (FP1-FP7) were characterized using Infrared spectroscopy, proton nuclear magnetic spectroscopy, and mass spectroscopy and evaluated for their antibacterial potential against Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and Gram-positive (Bacillus subtilis, Staphylococcus aureus) bacterial strain by measuring minimum inhibitory concentration values. Among the synthesized compounds, FP6 was found to be the best antibacterial agent having concentration values of 3.12 and 6.25 μg/ml against …

The Synergistic Antitumor Effects Of Curcuma Longa And Phyllanthus Niruri Extracts On Promoting Apoptotic Pathways In Breast Cancer Stem Cells, Dedy Hermansyah, Delfitri Munir, Aznan Lelo, Agung Putra, Nur Dina Amalina, Iffan Alif

The Thai Journal of Pharmaceutical Sciences

Breast cancer stem cells (BCSCs) develop apoptosis resistance by expressing pro- and anti-apoptotic protein including caspase-3, p53, and survivin. However, the induction of apoptosis is an important mechanism of action for many anticancer agents. Therefore, treatment using combination therapy was supposed to inhibit apoptosis resistance and increase the possibility of cancer apoptosis. This study aimed to investigate the effects of the combination Curcuma longa (CL) and Phyllanthus niruri (PN) extract on apoptosis induction. The single and combination cytotoxicity was analyzed using MTT assay. Apoptosis was measured by Annexin V-Propidium Iodide under flow cytometry. Caspase 3 expression was assessed by flow …

Carbon Dots: An Updated Overview On Their Synthesis, Properties, And Applications In Pharmaceutical And Biomedical Sciences, Earle Radha Rani, Majji Usharani, A. Lakshmi Usha

The Thai Journal of Pharmaceutical Sciences

Nowadays, carbon-based materials have acquired more attention in numerous fields including engineering, medicine, and pharmaceutics. As a new class of small carbon nanomaterials, carbon dots are developed due to their excellent physicochemical properties such as spherical shape, non-toxicity, cost effectiveness, facile, biocompatibility, and discrete particles <10 nm in diameter. In 2004, these carbon dots were first discovered accidentally during processing of single walled carbon nanotubes. Carbon dots have created huge excitement due to their advantages such as high water solubility, excellent biocompatibility, and resistance to photo bleaching. Carbon dots show unique properties such as photo stability, low toxicity, fluorescence, photo chemiluminescence, and electrochemiluminescence. Due to these properties, they have a wide range of applications in the field of bioimaging and drug delivery. Carbon dots are classified into two groups: Amorphous based carbon dots and graphene-based quantum dots with sp2 hybridization. The synthesis methods of carbon dots were reviewed along with their advantages and disadvantages. The applications of carbon dots in drug delivery, gene delivery, targeted drug delivery, crossing blood–brain barrier, and bio imaging were discussed.

Insights Into Novel Therapeutic Targets And Molecular Aspects In Polycystic Ovarian Syndrome: A Review, Rukaiah Fatma Begum, M. Sumithra, V. Chitra, Sharmila Devi

The Thai Journal of Pharmaceutical Sciences

Polycystic ovarian syndrome (PCOS) is a common endocrine syndrome in women associated with many other complications such as insulin resistance, obesity, hirsutism, increased risk of cardiovascular diseases, and infertility. As the pathophysiology of the syndrome still is not clear, it is difficult for the health-care professionals to precisely treat the syndrome. The cause of this syndrome is also unknown, but the evidences suggest that the factors such as environment, diet, and lifestyle are also a very common cause now a day for the syndrome. Recent pathophysiology of PCOS based on the Anti-Mullerian Hormone (AMH) and melatonin levels has been seen …

Efficacy Of Investigative Candidate Drugs Against Sars Cov-2: An Update On Pre-Clinical And Clinical Trials, Maryam Bibi, Aatika Sadia, Muhammad Shahzad, Ahmad Irfan, Muhammad Asim Raza Basra

The Thai Journal of Pharmaceutical Sciences

The unprecedented challenges presented by COVID-19 pandemic demands for an urgent development of effective therapeutic strategies against the deadly virus. Researchers thus far have revealed outcomes of pre-clinical and clinical studies of different interventions. This study aims to present overall summary of therapeutics that until this date are reported for their efficacy against COVID-19 in pre-clinical and clinical trials. A thorough literature search was conducted using databases for identification of studies published on the severe acute respiratory syndrome CoV (SARS COV-2) treatment. According to pre-clinical experiments, Nefamostat mesylate, Remdesivir, Chloroquine (CQ), Hydroxychloroquine (HCQ), β-D-N4-hydroxycytidine (NHC EIDD-1931), Ivermectin, Boceprevir, Calpain inhibitors, …

Assessment Of Risk Factors And Prescription Analysis Among Embolic And Atherosclerotic Stroke Patients In A Charitable Hospital-A Retrospective Study, Shanty Mary Jose, Amala Johnson, Akshay Abraham, Uday Venkat Mateti, Adithi Kellarai, Raushan Kumar Chaudhary, Shraddha Shetty

The Thai Journal of Pharmaceutical Sciences

Purpose: Despite the burden of stroke globally, there is a lack of data on the current clinical profile of stroke in middle and low-income countries including, India. Hence, the purpose of this study was to identify risk factors, clinical presentations, and importantly, prescription trends in ischemic stroke patients. Methods: A retrospective study was conducted for six months using data of 350 ischemic stroke patients for the analysis of risk factors and prescription pattern. Results: The incidence of ischemic stroke was higher (66%) in males in compared to (34%) females with mean age of 61.07 ± 11.460. Further the atherosclerotic stroke …

Hepatotoxic And Nephrotoxic Effects Of Chronic Exposure To Vernonia Cinerea Crude Extract On Wistar Rats, Thularath Toommakorn, Sajee Waramit, Patchara Danaisawadi, Kantimanee Pradermwong

The Thai Journal of Pharmaceutical Sciences

Vernonia cinerea L. (VC) has been used for smoking cessation and decreased appetite in Thailand. However, the safety of this plant concerning long-term consumption has never been reported. A 6-month toxicity study of VC water crude extract (VCWE) was performed in both genders of Wistar rats at doses of 15, 150, and 1500 mg/kg body weight/day orally. Recovery group received 1500 mg/kg for 6 months and then extended for 14 days without the extract. It was found that the males could not tolerate the highest dose of VCWE. There was no effect on bodyweight, relative organ weight, nor hematological parameters …

Anti-Diabetic Effect Of Interruptins A And B From Cyclosorus Terminans On Mouse Hepatocytes, Sujinda Songtrai, Sireewan Kaewsuwan

The Thai Journal of Pharmaceutical Sciences

Interruptin B from the fern Cyclosorus terminans has been indicated as a ligand of peroxisome proliferator-activated receptor (PPAR)-α and -γ that is known to regulate glucose homeostasis. Interruptin B was previously been implicated to promote glucose consumption into adipocytes. This study aimed to examine the anti-diabetic impact of interruptins A and B on glucose uptake and glycogen synthesis in mouse hepatocytes. Cell viability of FL83B hepatocytes affected by interruptins A and B was determined. The glucose consumption after 12–24 h treatment with compounds was evaluated by glucose oxidase method. The glycogen content was assessed after 24 h incubation using anthrone …

Alcl3 Induced Neuroinflammation And Excitotoxicity In Brain Striatum Mediated Memory And Motor Dysfunction Ameliorated Via Scopoletin Enriched Canscora Decussata Extract, A. Roja, T. Ambiga, Abdul Khayum, Sulaiman Mohammed, Venkatesh Gunasekaran

The Thai Journal of Pharmaceutical Sciences

Objective: Scopoletin is one of the dynamic and active constituents originate in Canscora decussata (CD) herb from Shankhpushpi family according to Indian system of medicine. This study aimed to characterize and to explore the role of scopoletin enriched CD (SECD) extract on AlCl3-induced neuroinflammation and excitotoxicity in rat model. Methodology & Results: High-performance thin-layer chromatography finger printing of ethanol extract of CD confirmed that enriched scopoletin presence as its main constituent. AlCl3 induced behavioural changes were reversed after SECD (200 and 400 mg/kg, i. p) and Rivastigmine (0.3 mg/kg, i.p) treatment by the way of Y maze, Rota rod, and …

Anti-Inflammatory Activity Of Phenolic-Rich Fraction Of Pandanus Tectorius Fruits Via Reduction Of No Production On Lipopolysaccharide-Stimulated Raw264.7 Cell Lines And Formalin-Induced Paw Oedema, Yosie Andriani, Nadia Madihah Ramli, Noor Suryani Musa, Kanwal Kanwal, Habsah Mohamad, Hermansyah Amir, Murni Nur Islamiah Kassim, Desy Fitrya Syamsumir, Tengku Sifzizul Tengku Muhammad, Suvik Assaw

The Thai Journal of Pharmaceutical Sciences

Background: The current study is the first study that investigated the potential of phenolic-rich fraction of Pandanus tectorius fruits as an anti-inflammatory agent. The fruit is rich in phenolic compounds, especially from the flavonoids group. Phenolics are known to contribute to an anti-inflammatory activity. Objectives: The first objective of the study was to evaluate the anti-inflammatory potency of phenolic-rich fractions of P. tectorius fruits by in vitro and in vivo studies. The second was to investigate the cytotoxicity against RAW264.7 cell lines, antioxidant, and phenolic contents to support the experiment. Materials and Methods: Sample fractionation was done with hexane, ethyl …

Antioxidant And Nephroprotective Effects Of Delphinium Denudatum Root Extract (Medicinal Plant) Against Gentamicin-Induced Nephrotoxicity, Nadeem Ahmad Siddique

The Thai Journal of Pharmaceutical Sciences

Introduction and Objectives: Kidney is a front line organ exposed to the toxicity of gentamicin. The objective of this study was to determine the biopotential of Delphinium denudatum Wall., root a significant medicinal plant, against gentamicin. Materials and Methods: By performing high-performance liquid chromatography, an in vitro antioxidant study was conducted against diphenylpicrylhydrazide (DPPH). In vivo nephroprotective activity was evaluated by administering solo administration of gentamicin (GM; 40 mg/kg b.wt. single dose s.c.) for a period of 1st–13th days followed by hydroalcoholic extract of D. denudatum (HAEDD; 200, 400, and 800 mg/kg b.wt. intragastrically) to the rats for 14th–23rd days. …

Antidiabetic Study And Teratogenicity Evaluation Of Ethanolic Extract Of Chromolaena Odorata Linn., Indah Solihah, Yuni Fitriani, Meutia Reni Noprida, Herlina, Riana Sari Puspita Rasyid, Tri Suciati

The Thai Journal of Pharmaceutical Sciences

There is considerable interest in herbal medicine in developing antidiabetic alternative therapy based on plant-derived compounds that would reduce oral hypoglycemic agent-related adverse effects while staying safe during pregnancy. The purpose of this in vivo study was to evaluate the ethanolic extract of Chromolaena odorata L. leaves (ECOL) in alloxan-induced diabetes and its teratogenic effect. The hypoglycemic impact of ECOL increased when the extract dose was raised. Three doses of ECOL (125, 250, and 500 mg/kg body weight) showed a 27.29%, 34.68%, and 52.24% hypoglycemic effect, respectively. While, the standard insulin (1 IU/kg BW) group shown a 31.38% hypoglycemic effect …

Synthesis And In Silico Study Of New Azetidinones Against Non-Small Cell Lung Cancer By Egfr Inhibition, Jainey P. James, T. C. Aiswarya, Swetha P. Shenoy, Vinod Devaraji

The Thai Journal of Pharmaceutical Sciences

Background: Lung cancer is alarmingly increasing and therapies targeting receptors such as human epidermal growth factor have been found to be emerging in treating lung cancer. Many studies report azetidinone bearing compounds to be cytotoxic. This work aims to prove the anti-lung cancer action of synthesized azetidinones that are through epidermal growth factor receptor (EGFR) inhibition. Materials and Methods: Novel azetidinone derivatives were synthesized from Schiff bases; molecular docking analyzed the interactions with EGFR, and pharmacophore modeling revealed the features responsible for the possible interactions. Moreover, the compounds with good docking scores were selected for anti-lung cancer study by MTT …

Mental Health, Social Skills, And Associated Factors Of Flight Attendants In A Commercial Airline In Thailand, Thanradee Kongmanee, Peeraphon Lueboonthavatchai

Chulalongkorn Medical Journal

Background: Mounting evidence has shown that most flight attendants have an average mental health and mental health problems such as stress, fatigue, and sleep problems. However, there were limited studies concerning their social skills and associated factors. This study identified the flight attendants’ mental health, associated factors with the mental health of flight attendants in Thai Airways International Public Company Limited. Objective: To explore the mental health, social skills, and associated factors of flight attendants in Thai Airways International Public Company Limited. Methods: A cross-sectional descriptive study was performed with the participation of 408 flight attendants of Thai Airways International …

Morphometric Measurements Of Posterior Cranial Fossa And Craniovertebral Junction In Chiari I Malformation With And Without Syringohydromyelia, Pawitra Imemkamon, Supada Prakkamakul, Ketsuda Jakchairoongruang

Chulalongkorn Medical Journal

Background: Syringohydromyelia occurs about 30.0 - 85.0% in patients with Chiari I malformation (CMI). The syrinx formation is supposed to be a result of disturbed cerebrospinal fluid flow dynamics, which is associated with the degree of mechanical blockage that may be related to posterior cranial fossa (PCF) and craniovertebral junction anatomy. Objective: Our study aimed to determine the morphometric difference in PCF and craniocervical junction between the CMI patients with and without syringohydromyelia. Methods: Thirty CMI patients (16 with syringohydromyelia; 14 without syringohydromyelia) and 16 healthy subjects were also recruited. PCF and craniocervical junction measurements of 5 distances and 5 …

Reliability Of The Thai Version Of The Modified Start Back Screening Tool In Individuals With Neck Pain, Nattawan Phungwattanakul, Uchukarn Boonyapo, Taweewat Wiangkham

Chulalongkorn Medical Journal

Backgrounds: The modified STarT Back Screening Tool in Thai version (mSBST-TH) for individuals with neck pain (NP) was adapted from the STarT Back Screening Tool in Thai version (SBST-TH) for individuals with low back pain (LBP) to classify individuals with NP into low, medium, and high risk for chronicity. Owing to a lot of individuals with NP in Thailand. It would be beneficial if the mSBST-TH was available to help primary care management in Thai individuals with NP. However, the reliability of this tool has not been investigated. Objective: The aim of this cross-sectional study was to investigate the reliability …

Acute Effect Of Low-Dose Sugar Ingestion On Forearm Blood Flow In Healthy Men, Manta Korakot, Weerapat Kositanurit, Pachara Varachotisate, Chuti Burana, Chanchai Boonla, Onanong Kulaputana

Chulalongkorn Medical Journal

Background: Hyperglycemia is a major risk factor for cardiovascular disease resulting in vascular damage in both diabetic and non-diabetic people. Objective: To determine acute effect of elevated plasma glucose from a low-dose sugar ingestion on forearm blood flow (FBF) in healthy men. Methods: Ten healthy men (aged 24.1 4.1 years) participated in two experimental conditions: 1) drinking 200 mL of water (Control), and 2) drinking 15 grams of sugar solution (Sugar). The two conditions were scheduled in random order with a minimum of three days of washout period between conditions. FBF was determined by venous occlusion plethysmography. Peak forearm blood …

Cyberbullying, Well-Being And Mental Health Of Freshmen Of A University In Bangkok, Thachira Jamnongrit

Chulalongkorn Medical Journal

Background: Cyberbullying is an electronic harassment commonly found in adolescents and affects the wellbeing and mental health of the victims. Objective: The purpose of this study was to estimate the prevalence of cyberbullying among freshman and to investigate the relationship between cyberbullying, psychological well-being, and mental health among freshmen of Chulalongkorn University. Methods: A cross-sectional design enrolled participation of 1,433 freshmen in 19 faculties of Chulalongkorn University. The subjects were requested to complete online surveys concerning demographic information, cyber-aggression victimization scale (Thai version), Thai mental health questionnaire (TMHQ: CU-modified version), and CU student psychological well-being (revised 2020). Results: Fifty-two point-seven …

Intra-Rater And Inter-Rater Reliability And Minimum Detectable Change Of Visual Analog Scale And Digital Goniometer In Patients With Subacute Unilateral Lateral Ankle Sprain, Thongchai Suksri, Chitanongk Gaogasigam, Sujitra Boonyong

Chulalongkorn Medical Journal

Background: Patients with subacute unilateral lateral ankle sprain commonly experience pain and limited range of motion (ROM). Measurement reliability should be investigated before assessing treatment effectiveness. Objective: To determine both intra-rater and inter-rater reliability and minimal detectable change (MDC) of visual analog scale (VAS) and digital goniometer in patients with subacute unilateral lateral ankle sprain. Methods: Ten subjects (7 men, 3 women; aged 29.90  12.81 years) with subacute unilateral lateral ankle sprain were recruited. Two investigators measured pain intensity and ROM using VAS and a digital goniometer, respectively, with a 30-minute break between measurements. The same protocol was repeated …

Social Media Addiction And Self-Esteem Of High School Students In Hat Yai Municipality, Secondary Educational Service Area Office 16, Thailand, Montira Muanjan, Sookjaroen Tangwongchai

Chulalongkorn Medical Journal

Background: Social media use has been increasing dramatically. Some evidences suggest that excessive use of social media may impact the level of self-esteem. Objective: To explore the level of social media addiction, self-esteem and associated factors among high school students in Hat Yai municipality. Methods: A cross-sectional descriptive design was employed with participation of 415 high school students in Hat Yai municipality. The instruments included a set of questionnaires to assess the demographic data and patterns of social media use, media addiction test (SMAT), Thai version of the Pittsburgh Sleep Quality Index (T-PSQI), Center for Epidemiological Studies-Depression Scale (CES-D) and …

Efficacy Of Digitalized Comprehensive Educational Program For Patients With Allergic Contact Dermatitis: A Randomized Controlled Trial, Wongsiya Viarasilpa, Prattana Sittiwattanawong

Chulalongkorn Medical Journal

Background: Allergic contact dermatitis (ACD) is an inflammatory skin disease resulting from exposure to allergen. The patient education is crucial to successful treatment and management of ACD. However, this process can be time consuming, requiring trained healthcare personnel resulting in increased workload and may be difficult in the setting of limited workforce or resource. Objective: To assess the effect of informative digitalized video-based educational program (iDVE) on patients’ quality of life, disease severity and knowledge of ACD compared to standard counselling (control). Methods: Patients with ACD were randomized to participate in iDVE or control group. The primary outcome was the …

Occupational Stress And Related Factors Among Registered Nurses At Relief And Community Health Bureau, The Thai Red Cross Society, Itsaraporn Palasak, Siriluck Suppapitiporn

Chulalongkorn Medical Journal

Background: Previous studies have found that occupational stress affects work performance among registered nurses and causes a high turnover rate. The study of occupational stress levels and related factors can help prevent occupational stress impacting on physical and mental health. Also, it may help increase work efficiency and enhance the commitment to the organization. Objective: To study the level of occupational stress and related factors among registered nurses at Relief and Community Health Bureau, The Thai Red Cross Society (RCHB-TRC). Methods: This was a cross-sectional study conducted at the RCHB-TRC, Thailand. Data were collected from 133 registered nurses using self-administered …

Development Of A Questionnaire To Evaluate The Attitudes Towards Home Health Visits In Applied Thai Traditional Medical Students: A Pilot Study, Sarunthorn Puttipaibool, Athippat Tantiwongsekunakorn, Kaittiyos Kuldejchaichan, Sukree Kade, Sirikan Poopong, Photnicha Jaturapat, Sasima Tongsai, Suprapath Sonjaipanich, Pravit Akarasereenont

Chulalongkorn Medical Journal

Background: Home health visits (HHV) are the new duty and development of students of applied Thai traditional medicine (ATM). We performed to develop a measuring instrument to ensure that students have positive attitudes to HHV. Objective: To develop a reliable and valid questionnaire for evaluating attitudes regarding home health visits among ATM students. Methods: There are 28 items in creating the questionnaire which is supposed to be used to assess attitudes toward HHV in four areas, including those related to the practice in HHV, the role of ATM practitioners, relationship with patients and patient’s relatives, and collaboration with the multidisciplinary …

Factors Influencing Decision Making To Use Large Private Hospitals Of Thai Patients In Bangkok After Covid-19 Outbreak, Decha Lalitanantpong, Kingporn Thongbai, Anothai Ngamvichaikit

Chulalongkorn Medical Journal

Background: The worldwide pandemic of coronavirus infections disease 2019 (COVID-19 impacts the Thai medical’s hub tourism industry. The private hospital sector needs to provide appropriate services for Thai consumers. Objectives: The objectives of this research were to study: 1) The level of decision making of use large private hospitals of Thai patients living in Bangkok after COVID-19 outbreak; 2) The marketing mix related to the level of decision making to use large private hospitals; 3) The marketing mix that influences decision making to use large private hospitals. Methods: This study was a survey research. The population was 8,757,843 Thai patients …

Changes In Brain Structural Volume And White Matter Abnormalities In Young Perinatally-Acquired Hiv Infected Children Treated With Antiretroviral Therapy, Netsiri Dumrongpisutikul, Kultida Chaiyagool

Chulalongkorn Medical Journal

Background: Several studies have reported human immunodeficiency virus (HIV) effects on brain volume and found white matter signal abnormality (WMSA) on brain magnetic resonance imaging (MRI). Objective: To evaluate brain volume and WMSA of antiretroviral therapy (ART)-treated perinatally-acquired HIV infected (PHIV) young children. Methods: From November 2016 to March 2018, MRI of 19 ART-treated PHIV young children, aged 12 - 56 months, were analyzed for structural brain volume using FreeSurfer software with manual correction. WMSA were classified into 4 grades. Comparison between the brain volumes and WMSA between early and late ART-treated groups as well as changes of the brain …

Workplace Innovation Management Of Nurse Managers In University Hospitals: A Phenomenological Study, Siriwan Thongthip, Kannikar Chatdokmaiprai, Phechnoy Singchungchai

Chulalongkorn Medical Journal

Background: University Hospitals in Thailand still have no responsible unit related to Workplace Innovation Management (WPIM) and First-Line Nurse Managers (FLNMs) are the key person of the organization, but today there is still a lack of knowledge on individual innovation and creativity in the workplace, especially in nursing organizations with the future practice of nursing in a technologically advanced future and keep up with modern society and digital technology transformation era. Objectives: To describe the definition and components of Workplace Innovation Management from First-Line Nurse Managers’ experiences in University Hospitals. Methods: Data were collected by in-depth interviews of FLNMs. Colaizzi’s …

The Coincidence Of Abernethy Malformation And Blue Rubber Bleb Nevus Syndrome: A Case Report, Thipsumon Tangsiwong, Siriwan Wananukul, Panruethai Trinavarat

Chulalongkorn Medical Journal

Abernethy malformation was demonstrated in a 10-year-old boy, whose underlying disease of blue rubber bleb nevus syndrome (BRBNS) was achieved first-time-right diagnosis by cutaneous stigmata, presenting with persistent gross hematuria and hemodynamic instability secondary to vesical venous malformation. Abdominal computed tomography (CT) imaging was obtained to explore identifiable and treatable causes of the active bleeding, revealing Abernathy malformation and unusual visceral slow-flow vascular malformations including venous varicosities and splenic lymphangiomatosis. This is a first case report of the coincidence of Abernathy malformation and BRBNS. Imaging played an important role in detection of clinically occult congenital portal venous anomalies and visceral …

Iranian Health Transformation Plan In Physiotherapy Services: Comparison Of Household Based Financial Outcomes, Seyedeh Mitra Hosseini Malekroudi, Zahra Mohtasham-Amiri, Enayatollah Homaie Rad

Journal of Health Research

Background: Physiotherapy services are important because they help disabled patients to return to work and other social activities. High out-of-pocket payments of physiotherapy services are one of the major barriers to the utilization of these services. In 2014 a reform was implemented in Iran named as Health Transformation Plan aimed to improve Universal Health Coverage of Iranians. We tried to find the effects of HTP on the financial outcomes of physiotherapy services.

Method: Data of Iranian Household Income and Expenditures Survey (IHIES) before and after HTP was used in this study. Out of Pocket payments and utilization of physiotherapy services …

Oral Administration Of Iron And Toltrazuril In Combination Improves Survival Rate And Growth Rate And Reduces Anaemia In Piglets, Pachara Pearodwong, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The present study was performed to determine the efficacy of an oral administration of iron and toltrazuril in combination on the growth rate, survival rate and the incidence of anaemia in suckling piglets. A total of 406 piglets were randomly allocated to one of three groups: i.) CONTROL (n = 161), the piglets were administered iron dextran 200 mg intramuscularly and toltrazuril (20 mg/kg) orally at 3 days of age; ii.) NEGATIVE (n = 84), the piglets were not treated with iron until 7 days of age, and toltrazuril was given at 5 days of age and iii.) TREATMENT (n …

Generation Of Tail Bearing Sperm-Like Cells From In Vitro Spermatogenesis Of Farming Goat Testis, Rakesh Bhaskar, Bijaylaxmi Sahoo, Sung Soo Han, Mukesh Kumar Gupta

The Thai Journal of Veterinary Medicine

In vitro spermatogenesis offers a therapeutic modality for the treatment of male infertility associated with pre-or post-meiotic barriers to the spermatogenesis process. The present study reports the production of tail-bearing sperm-like cells from in vitro spermatogenesis of farming goat testis in an air-liquid inter-phase culture. The testicular fragments (1-2 mm3) were placed on agarose scaffolds at air-liquid interphase and cultured in vitro for 60 days. Proliferating germ cell colonies were visible after 45-55 days of culture. Differentiation of cells to spermatogonia and spermatocytes was observed from the 40th day onwards. Tail bearing sperm-like elongated cells, with apical acrosome, were detected …