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Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

The Response Of Marine Synechococcus To A Landscape Of Environmental Stressors: A Proteomic Exploration, Dana E. Michels

Master's Theses

In the field of marine microbial ecology, many questions remain unanswered with regards to the physiological trade-offs made by phytoplankton to maximize growth (e.g., nutrient acquisition) and minimize loss (e.g., predation defenses). These tradeoffs, which occur at the cellular level, have wide reaching impacts on food web dynamics and global biogeochemical cycles. In the first chapter, we explored the use of a non-canonical amino acid (NCAA) technique, bioorthogonal non-canonical amino-acid tagging (BONCAT), in phytoplankton model systems. This technique has potential to work well in natural systems by enabling isolation of only newly synthesized proteins during an incubation period with the …

Machine Learning Approaches To Historic Music Restoration, Quinn Coleman

Master's Theses

In 1889, a representative of Thomas Edison recorded Johannes Brahms playing a piano arrangement of his piece titled “Hungarian Dance No. 1”. This recording acts as a window into how musical masters played in the 19th century. Yet, due to years of damage on the original recording medium of a wax cylinder, it was un-listenable by the time it was digitized into WAV format. This thesis presents machine learning approaches to an audio restoration system for historic music, which aims to convert this poor-quality Brahms piano recording into a higher quality one. Digital signal processing is paired with two machine …

The Effect Of Focused Ultrasound On Altering The Diameter Class Of Nerve Fibers Contributing To A Compound Evoked Potential, Analyzed Using A Perturbative Decomposition Technique., Megan Wurden

Master's Theses

Peripheral neuropathies are disorders that involve the damage of peripheral nerve fibers, affecting the ability of different parts of the body to communicate. A differentiating factor in diagnosis between various clinical conditions can be which size class of nerve fibers are affected. A nerve conduction velocity test can be used to assess the viability of the nerve but is a single-parameter test and gives no information about the population characteristics of the remaining active fibers. A method developed and previously reported by Szlavik (2016) utilizes a mathematical perturbed decomposition to determine the normalized frequency of each size class of fiber …

Development Of A Hybrid Particle Continuum Solver, Anthony J. Gay

Master's Theses

When simulating complex flows, there are some physical situations that exhibit large fluctuations in particle density such as: planetary reentry, ablation due to arcing, rocket exhaust plumes, etc. When simulating these events, a high level of physical accuracy can be achieved with kinetic methods otherwise known as particle methods. However, this high level of physical accuracy requires large amounts of computation time. If the simulated flow is in collisional equilibrium, then less computationally intensive continuum methods, otherwise known as fluid methods, can be utilized. Hybrid Particle-Continuum (HPC) codes attempt to blend particle and fluid solutions in order to reduce computation …

Towards A Complete Formal Semantics Of Rust, Alexa White

Master's Theses

Rust is a relatively new programming language with a unique memory model designed to provide the ease of use of a high-level language as well as the power and control of a low-level language while preserving memory safety. In order to prove the safety and correctness of Rust and to provide analysis tools for its use cases, it is necessary to construct a formal semantics of the language. Existing efforts to construct such a semantic model are limited in their scope and none to date have successfully captured the complete functionality of the language. This thesis focuses on the K-Rust …

Early Wildfire Detection With Line Sensors, Virginia Yan

Master's Theses

Over the last few years, wildfires have become more devastating to communities as the fires are inevitably destructive to many homes, businesses, and ecosystems. Frequent wildfires also pose a significant threat to power grids and nearby residents as they can damage transmission lines and other electrical equipment, which in turn can cause major power shutdowns. Especially in western U.S., severe drought conditions and weather variability cause residents to become more vulnerable to wildfire disasters as their safety is threatened. We are incompetent to control the wildfires effectively despite existing advanced technologies. Hence, an algorithm based on energy conservation and heat …

Development Of A Robust Methodology To Obtain And Assess Myogenic Precursor Cells For Their Use In Regenerative Therapies, Ricardo Lasa

Master's Theses

Peripheral arterial occlusive disease (PAOD) is characterized by buildup of atherosclerotic plaque in peripheral arteries that leads to an occlusion that can interrupt the supply of blood to the peripheral tissue, causing downstream tissue ischemia/hypoxia. PAOD is estimated to affect over 200 million patients worldwide. Current surgical revascularization treatments can be effective in about half of the patient population, leading to a significant number of patients with no treatment options beyond pharmacological intervention and lifestyle modification. The decrease in blood flow downstream of the occlusion leads to increased blood pressure gradient in the microvasculature, specifically in vessels that connect arterial …

Brain Tumor Detection And Classification From Mri Images, Anjaneya Teja Sarma Kalvakolanu

Master's Theses

A brain tumor is detected and classified by biopsy that is conducted after the brain surgery. Advancement in technology and machine learning techniques could help radiologists in the diagnosis of tumors without any invasive measures. We utilized a deep learning-based approach to detect and classify the tumor into Meningioma, Glioma, Pituitary tumors. We used registration and segmentation-based skull stripping mechanism to remove the skull from the MRI images and the grab cut method to verify whether the skull stripped MRI masks retained the features of the tumor for accurate classification. In this research, we proposed a transfer learning based approach …

Development Of Novel Hardware And Software For Wind Turbine Condition Monitoring, Ryan A. Zhan

Master's Theses

With the increased use of wind turbines as sources of energy, maintenance of these devices becomes more and more important. Utility scale wind turbines can be time consuming and expensive to repair so an intelligent method of monitoring these devices is important. Commercial solutions for condition monitoring exist but are expensive and can be difficult to implement. In this project a novel condition monitoring system is developed. The priority of this system, dubbed the LifeLine, is to provide reliable condition monitoring through an easy-to-install and low-cost system. This system utilizes a microcontroller to collect acceleration data to detect imbalances on …

Experimental Investigation Of The Effects Of Acoustic Waves On Natural Convection Heat Transfer From A Horizontal Cylinder In Air, Katherina V. Prodanov

Master's Theses

Heat transfer is a critical part of engineering design, from the cooling of rocket engines to the thermal management of the increasingly dense packaging of electronic circuits. Even for the most fundamental modes of heat transfer, a topic of research is devoted to finding novel ways to improve it. In recent decades, investigators experimented with the idea of exposing systems to acoustic waves with the hope of enhancing thermal transfer at the surface of a body. Ultrasound has been applied with some success to systems undergoing nucleate boiling and in single-phase forced and free convection heat transfer in water. However, …

Ram Air-Turbine Of Minimum Drag, Raymond Akagi

Master's Theses

The primary motivation for this work was to predict the conditions that would yield minimum drag for a small Ram-Air Turbine used to provide a specified power requirement for a small flight test instrument called the Boundary Layer Data System. Actuator Disk Theory was used to provide an analytical model for this work.

Classic Actuator Disk Theory (CADT) or Froude’s Momentum Theory was initially established for quasi-one-dimensional flows and inviscid fluids to predict the power output, drag, and efficiency of energy-extracting devices as a function of wake and freestream velocities using the laws of Conservations of Mass, Momentum, and Energy. …

Gyroscopic Human Balance Device, Randy Lam Hau, Spencer Ross, Yael Farkash

Biomedical Engineering

This project’s sponsor, Charlie Guitierrez, has decades of experience building assistive walking devices for the wheelchair bound population. During his work, he noticed that many of his clients retained some ability to balance themselves and so theorized that they would be able to walk independently if given some form of assistance. This assistance could potentially be in the form of a wearable gyroscope. Gyroscopes are commonly used to stabilize objects subjected to instability, such as bicycles, motorcycles, and ships. Thus, a wearable gyroscope would assist its user in maintaining overall stability by imparting reactive torques and forces if the user …

Hypoxic Incubation Chamber, Makenzie Jones, Andrea Skattenborg, Henry Elsner

Biomedical Engineering

In developing a model to test anti-tumor drugs, Dr. Christopher Heylman’s lab requires the ability to culture cells in a hypoxic environment. The purpose of this project was to make this possible. Funding for this project comes from the Biomedical Engineering Department, the Hannah-Forbes Fund, and Dr. Heylman’s lab. The chamber must be able to reach a user-defined oxygen concentration and hold that concentration for 48 hours while creating an environment conducive to cell culture. Importantly, the chamber must also be sterilizable and cleanable for repeated use. While several products exist to create hypoxic environments for cell culture, they are …

Effect Of Prenatal Lifestyle Intervention On Maternal Postpartum Weight Retention And Child Body Massindex Z-Score At 36 Months, Suzanne Phelan, Chantelle N. Hart, Elissa Jelalian, Karen Muñoz-Christian, Noemi Alarcon, Angelica Mchugh, Alison K. Ventura, Rena R. Wing

Kinesiology and Public Health

Background/Objectives We previously reported results from a randomized trial showing that a behavioral intervention during pregnancy reduced excess gestational weight gain but did not impact maternal weight at 12 months. We now examine the longer-term effects of this prenatal intervention on maternal postpartum weight retention and toddler body-mass-index z scores (BMIz) over 36 months.

Subjects/Methods Pregnant women (N = 264; 13.7 weeks’ gestation; 41.6% Hispanic) with overweight or obesity were randomized into usual care or prenatal intervention. Anthropometric assessments in mothers and toddlers occurred at baseline, 35 weeks’ gestation and after delivery at 6, 12, 18, 24, and 36 …

As-912-21 Resolution On "Poly Access" Textbook Program, Ryan Jenkins

Academic Senate Resolutions

Resolution of Academic Senate urging the Cal Poly Bookstore to change the "Poly Access" program from opt-out to opt-in program, resolving the university engage in more consultation with faculty and student representatives in regards to bookstore decisions and ways be mindful of how decisions can negatively impact student finances.

As-910-21 Resolution On Updating The United States Cultural Pluralism (Uscp) Educational Objectives, Academic Senate

Academic Senate Resolutions

Adoption of updated USCP course criteria and educational objectives

Academic Senate - Minutes, 02/23/2021, Academic Senate

Academic Senate Minutes

No abstract provided.

Academic Senate - Agenda, 02/23/2021, Academic Senate

Academic Senate Agendas

No abstract provided.

As-911-21 Resolution On Faculty Choice Of Modality, Executive Committee

Academic Senate Resolutions

Extending AS-903-20 and AS-904-20 through Summer 2022, allowing instructional faculty to select their teaching modality as face-to-face or virtual

As-909-21 Resolution On New Academic Assessment Council Membership, Academic Senate

Academic Senate Resolutions

Approval of the Revised Membership for Cal Poly's Academic Assessment Council and effective immediately.

Executive Committee - Minutes, 02/16/2021, Executive Committee

Academic Senate Minutes

No abstract provided.

Executive Committee - Agenda, 02/16/2021, Academic Senate Executive Committee

Academic Senate Agendas

No abstract provided.

Review Of Lisa Kemmerer's Sister Species: Women, Animals, And Social Justice, Marine Lercier

Between the Species

What do we have in common with animals, and what do these women have in common? We are Sister Species, if not sisters at all. Lisa Kemmerer invites us to realize that we are more alike than different and to become aware of what our animal brothers and especially sisters experience: the suffering they endure because of our absurd inconsistencies and oppositions - even within the animal rights movement, often unbeknownst to us. The goal: more effective discourse and action, educating us to the other in the face of a norm imposed by a power, a discourse of normalization …

As-908-21 Resolution On University Faculty Personnel Policies Subchapter 12.4: Sabbatical And Difference In Pay Leaves, Academic Senate Faculty Affairs Committee

Academic Senate Resolutions

Sabbatical policies will be updated by Fall 2021 in University Faculty Personnel Policy documents.

Academic Senate - Agenda, 02/09/2021, Academic Senate

Academic Senate Agendas

No abstract provided.

Academic Senate - Minutes, 02/09/2021, Academic Senate, Academic Senate

Academic Senate Minutes

No abstract provided.

The Future Of Organic Insect Pest Management: Be A Better Entomologist Or Pay For Someone Who Is, David H. Headrick

Horticulture and Crop Science

Insect pest management in certified organic production systems presents considerable challenges for growers. The Federal National Organic Program (NOP) guidelines list acceptable tactics, but their effective use requires a considerable knowledgebase in entomology. The range of tactics allowed by the NOP are viewed as limiting by many growers and there are important elements missing from the list such as pest monitoring and identification. Educational programs must consider utilizing instructional methods and additional means of outreach that introduce new pest management tactics that are individualized, regionally appropriate and emphasize grower adoption and collaboration with local professionals. This review describes the challenges …

Import Restrictions By Eco-Certification: Quantity Effects On Tropical Timber Production, Matthew T. Cole, Jacqueline M. Doremus, Stephen F. Hamilton

Economics

Eco-certification standards are increasingly used by industrial countries to restrict imports of foreign goods produced using unsustainable practices. Import restrictions on eco-certified goods limit the trade of goods to the home country, but also serve to segment global demand into separate regions for conventional goods and certified goods, altering market structure and equilibrium prices in a manner that can work against sustainability goals. In this paper, we examine the effect of recent import restrictions in the US, EU, Canada, and Japan that require tropical timber products produced in Central Africa to be eco-certified. Using panel data of timber production in …

Comparative Effects Of Chemical And Physical Sunscreen On Fertilization Of Purple Sea Urchins (Strongylocentrotus Purpuratus), Marilla Lippert, Maurice Codespoti Goodman, Nikki Adams

Biological Sciences

Organic compound-based “chemical” sunscreens dominate the commercial sunscreen market, but recent research has revealed the ingredients of these products are detrimental to the health of marine organisms. This revelation has led to increased popularity of mineral-based “physical” sunscreens, primarily containing zinc-oxide (ZnO), as environmentally safe alternatives. While they are marketed as environmentally safe, these claims are largely untested, and it is important to consider potential effects of ZnO-based sunscreens on the development of marine organisms. Though Zn is a necessary micronutrient in the ocean, excess Zn is released into marine environments from anthropogenic sources has negative effects on marine life. …