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Heavy Metal-Induced Oxidative Stress On Seed Germination And Seedling Development: A Critical Review, Mihiri Seneviratne, Nishanta Rajakaruna, Muhammad Rizwan, H. M. S. P. Madawala, Yong Sik Ok, Meththika Vithanage

Biological Sciences

Heavy metal contamination in soils can influence plants and animals, often leading to toxicosis. Heavy metals can impact various biochemical processes in plants, including enzyme and antioxidant production, protein mobilization and photosynthesis. Hydrolyzing enzymes play a major role in seed germination. Enzymes such as acid phosphatases, proteases and α-amylases are known to facilitate both seed germination and seedling growth via mobilizing nutrients in the endosperm. In the presence of heavy metals, starch is immobilized and nutrient sources become limited. Moreover, a reduction in proteolytic enzyme activity and an increase in protein and amino acid content can be observed under heavy …

Customization Of Aneurysm Scaffold Geometries For In Vitro Tissue-Engineered Blood Vessel Mimics To Use As Models For Neurovascular Device Testing, Camille D. Villadolid

Master's Theses

Cerebral aneurysms occur due to the ballooning of blood vessels in the brain. Rupture of aneurysms can cause a subarachnoid hemorrhage, which, if not fatal, can cause permanent neurologic deficits. Minimally invasive neurovascular devices, such as embolization coils and flow diverters, are methods of treatment utilized to prevent aneurysm rupture. The rapidly growing market for neurovascular devices necessitates the development of accurate aneurysm models for preclinical testing. In vivo models, such as the rabbit elastase model, are commonly chosen for preclinical device testing; however, these studies are expensive, and aneurysm geometries are difficult to control and often do not replicate …

Mechanisms Of Reduced Vascular Tone Following Arteriogenesis Induced By Femoral Artery Ligation, Christopher Hatch

Biomedical Engineering

The presence of a developed, native collateral network can decrease the severity of ischemic injury proceeding arterial occlusion. The collateral network must under arteriogenesis to enlarge and increase blood flow to the ischemic region. Although there has been tremendous effort attempting to understand the mechanisms of arteriogenesis, no therapies have been successful in improving patient outcome. To better understand the mechanisms involved in arteriogenesis, the effect of nitric oxide production, myogenic tone, and a-adrenergic receptors were evaluated as these have been identified as playing an important role in vascular injury. Arteriogenesis was induced by ligating the femoral artery between the …

Children's Acceptability Of Vegetables: The Relationship Between Food Neophobia, Vegetable Neophobia, Picky Eating, Bitter Sensitivity, And Mouth Behavior, Tara Lynn Egigian

Master's Theses

Although vegetables provide many beneficial nutrients and have been shown to help reduce the risk of dietary related chronic diseases, children in the United States are not meeting the national recommendations of vegetable servings. The overall goal of this research was to study the relationship between children’s vegetable acceptance and the following children’s characteristics: food neophobia (FN), vegetable neophobia (VN), picky eating (PE), 6-n-propylthiouracil (PROP) sensitivity, Mouth Behavior (MB). The specific objectives were to: (1) develop a novel method for evaluating children’s MB, (2) assess the FN, VN, PE, PROP sensitivity, and MB levels in children in San Luis Obispo …

Biological Pathway Involvement In Melanoma Heterogeneity And Drug-Induced Resistance, Sarah V. Pack

STAR Program Research Presentations

Tumors develop resistance to numerous drug therapies, and this remains a major obstacle in treating many types of non-surgical cancers. Melanoma provides a good model system for studying drug resistance in cancer due to its high propensity to incur resistance after a significant initial response to a drug. Genes that are highly expressed in melanoma cancer cells have been studied, but in order to further understand the collective function of these highly expressed genes we must analyze gene sets, or pathways. A single gene’s function is rarely independent of other genes, and pathway analysis takes this into account.

Our objective …

Infestation Intensities, Attachment Patterns, And The Effect On Host Contest Behavior Of The Tick Ixodes Pacificus On The Lizard Sceloporus Occidentalis, Dylan M. Lanser

Master's Theses

Parasites often have profound effects on the survival and evolution of their hosts, and hence on the structure and health of entire ecosystems. Yet basic questions, such as the degree of virulence of a given parasite on its host, and factors influencing which hosts in a population are at the greatest risk of infection, are vexingly difficult to resolve. The western blacklegged tick-western fence lizard (Ixodes pacificus-Sceloporus occidentalis) system is important, primarily because I. pacificus, a vector of the Lyme disease spirochete Borrelia burgdorferi, is dependent on S. occidentalis for blood meals in its subadult stages, …

Examining The Effectiveness Of Electromyography Biofeedback At Improving The Upper Trapezius To Serratus Anterior Muscle Activation Ratio, Julia Evelyn Holton

Master's Theses

Purpose: The upper trapezius to serratus anterior muscle activation ratio is essential for optimal shoulder function. An alteration of this ratio, specifically a decrease in upper trapezius and increase in serratus anterior activation, is a main area of focus in shoulder rehabilitation (Kibler, 1998; Paine & Voight, 1993). Electromyography (EMG) biofeedback has been shown to be an effective rehabilitation technique to address many musculoskeletal disorders but there is limited research on the retention of improvements seen with EMG biofeedback (Ma et al., 2011; Lim et al., 2014; Weon, et al., 2011). The purpose of this study was to determine if …

Keeping Humans In The Loop: Pooling Knowledge Through Artificial Swarm Intelligence To Improve Business Decision Making, Lynn E. Metcalf, David A. Askay, Louis B. Rosenberg

Industrial Technology and Packaging

This article explores how a collaboration technology called Artificial Swarm Intelligence (ASI) addresses the limitations associated with group decision making, amplifies the intelligence of human groups, and facilitates better business decisions. It demonstrates of how ASI has been used by businesses to harness the diverse perspectives that individual participants bring to groups and to facilitate convergence upon decisions. It advances the understanding of how artificial intelligence (AI) can be used to enhance, rather than replace, teams as they collaborate to make business decisions.

Maternal Diet During Lactation And Breast-Feeding Practices Have Synergistic Association With Child Diet At 6 Years, Jacob P. Beckerman, Emily Slade, Alison K. Ventura

Kinesiology and Public Health

Objective: Children breast-fed during infancy consume more fruits and vegetables than formula-fed children. This pattern is likely due, in part, to infant learning from flavours of the mother’s diet transmitted through breast milk, but more research is needed to understand associations between early flavour exposures and later dietary patterns. We examined whether breast-feeding and maternal fruit and vegetable consumption during nursing were synergistically associated with higher child fruit and vegetable consumption.

Design: Prospective cohort study of breast-feeding duration, maternal diet postpartum and child diet. Complete breast-feeding and maternal diet data were available for 1396 mother–child dyads; multiple imputation was used …

The Five Tribes Of Machine-Learning: A Brief Overview, Jens G. Pohl

Architecture

This paper reviews recent advances in automated computer-based learning capabilities. It briefly describes and examines the strengths and weaknesses of the five principal algorithmic approaches to machine-learning, namely: connectionism; evolutionism; Bayesianism; analogism; and, symbolism. While each of these approaches can demonstrate some degree of learning, a learning capability that is comparable with human learning is still in its infancy and will likely require the combination of multiple algorithmic approaches. However, the current state reached in machine-learning suggests that Artificial General Intelligence and even Artificial Superintelligence may indeed be eventually feasible.

Partnering Without Boundaries: Integrating Structures, Architecture, Practice, And Education, Thomas Fowler, Iv, Kevin Dong

Architecture

A college wide, studio-based engineering and design course is used as the mechanism for learning, experimentation, and exploration of the built environment. Student teams part- ner with design professional mentors to create viable solutions for real world projects. Students address architectural, structural, environmental, and construct-ability issues. For the highlighted project, students integrated laboratory research and fundamental engineering principles to develop building designs which recognized current building technology, sustainability, and cultural issues for a polytechnic college in rural Tanzania; then based on the design proposals, constructed a building proto-type. The school motto is “Learn by Doing” and with projects such as …

Advanced Structural Integration Collaboratory Model For Architecture Students, Thomas Fowler, Iv

Architecture

The architecture program at Cal Poly, San Luis Obispo, California, has a strong technology component (as part of the Polytechnic Mission of the University) over the years since its inception in the 1960’s, that led to the establishment of the College of Architecture andEnvironmental Design. The paper presents an advanced structural integration collaboratory model that works with recent changes in the curriculum. The history of the program is important, since the curriculum has always had a very intensive architectural engineering (structural systems focus) sequence of courses. While architecture students were definitely acquiring a strong knowledge of a wide range of …

Integrated Aeration For Enhanced Nitrogen Conversion And Removal And The Effect Of Sampling Frequency On Productivity Estimates In Algae-Based Wastewater Treatment Systems, Lauren Parker

Master's Theses

Raceway ponds can be used to cultivate microalgae for purposes such as wastewater treatment and feedstock for animal feeds and biofuels. Besides nitrogen assimilation in biomass, nitrification can be a wastewater treatment mechanism but one that is hindered by the low nighttime dissolved oxygen concentrations (DO) common in wastewater treatment raceways. The large diel difference in pond conditions and changing influent wastewater quality also raises the question of the optimal frequency and timing of grab sample collection, to obtain representative water quality data. Three sets of triplicate pilot raceways (1,000-L, 0.30-m deep, fed primary clarifier effluent) were operated at an …

Development Of A Prolyl Endopeptidase Expression System In Lactobacillus Reuteri To Reduce The Clinical Manifestation Of Celiac Disease, Kara Lynn Jew

Master's Theses

Celiac Disease (CD) is an autoimmune disorder that emerges due to the ingestion of gluten, a protein found in a variety of common grains such as wheat, rye, and barley. Approximately 1 in 100 individuals in the US suffer from CD, making it the most commonly diagnosed gastrointestinal disorder (Ciclitira et. al., 2005). These proline-rich gluten peptides are resistant to proteolysis and accumulate in the duodenum of the small intestine. Once in the duodenum, these peptides illicit an autoimmune response resulting in villous atrophy. Current treatment for CD requires a rigorous adherence to a gluten-free diet. Nevertheless, gluten-containing grains are …

Data-Driven Database Education: A Quantitative Study Of Sql Learning In An Introductory Database Course, Andrew C. Von Dollen

Master's Theses

The Structured Query Language (SQL) is widely used and challenging to master. Within the context of lab exercises in an introductory database course, this thesis analyzes the student learning process and seeks to answer the question: ``Which SQL concepts, or concept combinations, trouble students the most?'' We provide comprehensive taxonomies of SQL concepts and errors, identify common areas of student misunderstanding, and investigate the student problem-solving process. We present an interactive web application used by students to complete SQL lab exercises. In addition, we analyze data collected by this application and we offer suggestions for improvement to database lab activities.

Short Term Shifts In Soil Nematode Food Feb Structure And Nutrient Cycling Following Sustainable Soil Management In A California Vineyard, Holly M. Deniston-Sheets

Master's Theses

Evaluating soil health using bioindicator organisms has been suggested as a method of analyzing the long-term sustainability of agricultural management practices. The main objective of this study was to determine the effects of vineyard management strategies on soil food web structure and function, using nematodes as bioindicators by calculating established nematode ecological indices. Three field trials were conducted in a commercial Pinot Noir vineyard in San Luis Obispo, California; the effects of (i) fertilizer type (organic and inorganic), (ii) weed management (herbicide and tillage), and (iii) cover crops (high or low water requirements) on nematode community structure, soil nutrient content, …

The Effects Of Edaphic And Climatic Factors On Secondary Lichen Chemistry: A Case Study Using Saxicolous Lichens, Alexander Paukov, Anzhelika Teptina, Maria Morozova, Ekaterina Kruglova, Sergio E. Favero-Longo, Cora Bishop, Nishanti Rajakaruna

Biological Sciences

Diversity of secondary lichen metabolites and their relationship to substrate and environmental parameters were studied in saxicolous lichens in the Middle and South Urals of Russia. Atranorin, usnic acid, gyrophoric acid, zeorin, norstictic acid, antraquinones and stictic acid were found in 73, 42, 41, 37, 36, 35 and 32 species, respectively, of 543 taxa collected. One hundred and ninety six species (i.e., 36% of total species documented) contained no secondary metabolites. Spectra of secondary metabolites of crustose lichens varied on different rock types, while in fruticose and foliose groups only those species without lichen acids were dependent on the substrate …

Tropic Transfer And Bioaccumulation Of Lead Along Soil-Plant-Aphid-Ladybird Food Chain, Mohod Irfan Naikoo, Mudasir Ifran Dar, Fareed Ahmad Khan, Fariha Raghib, N. Rajakaruna

Biological Sciences

Lead (Pb) contamination of agroecosystems is a serious issue as Pb is a persistent pollutant that is retained in soil for long, causing toxicities to organisms. This study examines biotransfer of Pb from soils treated with different concentrations of Pb through a broad bean (Vicia faba L.)–aphid (Aphis fabae Scop.)–ladybird (Coccinella transversalis Fabricius) food chain and its consequent inference for natural biological control, the ladybird. The soil was amended with Pb at the rates of 0, 25, 50, 75 and 100 mg kg⁻¹(w/w). The amount of Pb in plant, aphid and ladybird increased in a dose-dependent manner …

Influence Of Soil Water Content And Soil Amendments On Trace Metal Release And Seedling Growth In Serpentine Soil, Viraj Gunarathne, Nishanta Rajakaruna, Udaya Gunarathne, Jayanta Kumar Biswas, Zach A. Raposo, Meththika Vithanage

Biological Sciences

This study was conducted to evaluate the synergistic effects of organic amendments and soil water status on trace metal release from serpentine soil.

Academic Senate - Agenda, 6/04/2019, Academic Senate

Academic Senate Agendas

No abstract provided.

Impact Of Probiotics On Gastrointestinal Pathogenic Growth And Host Tight Junction Integrity, Rachel Cohen

Baker/Koob Endowments Awarded Projects

Over the past decade, interest in the use of probiotics, or “good bacteria”, for human health advancement and remediation has grown. Previous studies have shown that some health benefits conferred by probiotics are strain specific, therefore individual probiotic effects must be evaluated. This study evaluated some probiotic candidates on their ability to produce metabolites which inhibit the growth of foodborne pathogens. Probiotics are known to inhibit growth of other bacteria through competitive exclusion and production of bactericidins. In addition, previous studies have established that some probiotics promote the integrity of tight junctions, proteins that play a role in intestinal epithelial …

A Paper-Based Elisa Device For The Rapid Detection Of Ischemic Stroke, Andrea Larsen

Baker/Koob Endowments Awarded Projects

Diagnosing an ischemic stroke accurately and quickly is essential for appropriate treatment and results in more effective management of the stroke, leading to better patient outcomes. Specifically, the determination of ischemic versus hemorrhagic stroke leads to critically different treatment paths, which would be potentially fatal if administered incorrectly. Currently, ischemic strokes are diagnosed through a series of tests, including a physical examination, CT scans, and a panel of blood tests to exclude hemorrhagic stroke or other similarly presenting conditions, in order to administer tPA (the main treatment) within the three hour window. The discovery of biomarkers, molecules which are upregulated …

Optimizing Novel Cosmetic Formulations Using Natural Microbial Resistant Additives, Kiersten Smith, Stephanie Wong

Baker/Koob Endowments Awarded Projects

Today’s off-the-shelf cosmetics are filled with various chemicals and additives to produce a product that performs effectively for the consumer; however, the industry has been steering towards more natural products that are marketed to be safer and healthier for consumers. In particular, facial moisturizers have shown a shift from synthetic additives, such as benzoyl peroxide, to naturally found additives, including tea tree oil and aloe vera. Unfortunately, there is a lack of accessibility to data confirming or denying the effectiveness of the various additives used in cosmetic products. This study aimed to determine the performance differences, if any, among natural …

Accessible And Responsive Website Design For Cal Poly Dbs Marine Education Program, Charles W. Alexander

Computer Engineering

In this project, I demonstrate how accessible and responsive designs are followed in order to implement a modern, multi-page website which both adapts to the size of the screen as well as has the logical, semantic structure needed for accessible technologies to accurately use the site. This website is designed for a marine education program, Dive Beneath the Surface, which hosts live streams of scientific divers as they interact in real time with students many miles away. Although this site will not be the streaming platform, it needs to host a repository of videos and lessons for those students and …

Synthesis Of Molecularly Imprinted Polymers For Protein Detection And Sample Depletion, Anel Ruiz, Christopher Lausted

STAR Program Research Presentations

Molecular Imprint Polymers (MIPs) constitute a unique class of chemically-synthesized antigen recognition materials. As microparticles and nanoparticles, they can act as synthetic antibodies with numerous potential applications. We will develop protocols for the rapid generation of albumin-binding MIPs, characterize the affinity and specificity of the resulting particles, and investigate their application towards serum depletion for proteomics.

Compositional Optimization Of Amyloid-Graphene Oxide Nanohybrids For Biomaterials, Claire L. Drewery

Materials Engineering

Amyloid nanofibrils are natural materials capable of self-assembling into precise structures with tunable functionalities, while exhibiting excellent mechanical properties. In combination with highly conductive graphene oxide (GO), the 1-D amyloid nanofibrils and 2-D nanosheets of GO can produce a robust and bio-functional nanohybrid, hypothesized to exhibit multi-domain functional properties useful for enzyme sensing, water purification, drug delivery, and tissue scaffolding applications. Here, we examine the properties of an amyloid-graphene oxide nanohybrid film made with amyloids derived from hen egg white lysozymes in an attempt to explore the diverse toolbox of amyloid derivatives and establish ideal fabrication methods and formulations of …

Nano-Enhanced Composite Membranes For Water Desalination, Benjamin Fredrik Victor Sundling Von Fürstenrecht

Materials Engineering

In theory single walled carbon nanotubes (SWCNT) will aid in ion rejection due hydrophobicity and smoothness of the SWCNT. An efficient means of water desalination utilizing SWCNT in a membrane seems plausible. A lyotropic liquid crystal (LLC) solution was made with a synthesized polymerizable surfactant methacryloxy ethyl hexadecyl dimethyl ammonium bromide (C16MA) to help with vertical alignment of SWCNT. Due to SWCNT lack of solubility and tendency to agglomerate in water, a dispersion performed using an inert surfactant centrimonium bromide (CTAB) to make sure that the SWCNT were homogeneously dispersed in the solution without altering the hexagonal packing factor of …

Rescue And Rehabilitation Of A Juvenile Red-Tailed Hawk: A Case Study, Maya Higa

Agricultural Education and Communication

Many raptor species are becoming threatened and, in some cases, endangered as a result of human activity including habitat destruction and pesticide use (Morishita, Fullerton, Lowenstine, Gardner & Brooks, 1998). Because of this, raptor rehabilitation has become increasingly popular in conservation efforts. In North America, the red-tailed hawk is one of the most widespread and commonly known birds of prey (Preston & Beane, 2009). According to the National Audubon Society, the conservation status of red-tailed hawks is currently of least concern and numbers may even be increasing in some areas since the 1960s. While this species is not threatened, the …

Consumer Choice Within Fresh Produce, Victoria C. Ross

Agricultural Education and Communication

This project set out to question whether fresh produce required more specific labeling of pesticides and fertilizer inputs on fresh produce. Like many questions regarding health, environmental science, agriculture, and politics, there is not one simple answer. After listening to professionals and interviewing customers outside grocery stores, it's clear that the majority of consumers are not pursuing additional information, but that there is a small group of customers who would appreciate it. It also became clear that although fear mentality is of concern to professionals in the food industry, consumers don’t seem to understand the negative effects their participation in …

Investigation Of The Encapsulation Efficiency Of Hexanal In Y-Cyclodextrin Metal Organic Frameworks, Travis J. Lang

Materials Engineering

Nanoporous materials have been extensively studied for applications such as drug delivery and organic compound storage. The nanoporous material of this study is a metal organic framework (MOF) which is a coordination of metal ions with organic binders. The structure created by this coordination can be used to absorb organic compounds, such as plant growth regulators, and subsequently release the organic compounds over a prolonged period to extend the storage life of foods. This study is examining the encapsulation efficiency of hexanal in γ-cyclodextrin metal organic frameworks (γ-CDMOFs) as a mechanism for potential active packaging applications. γ-CDMOFs were synthesized by …