Digital Commons Network^™

Can Oral Contraceptives Increase Antibiotic Resistance In Escherichia Coli?, Allison Helene Clatch, Leah Van Otterloo

Baker/Koob Endowments Awarded Projects

Antibiotic resistance is one of the most pressing threats to public health. Intrinsic resistance to many antimicrobials in Gram-negative bacteria is accomplished by naturally occurring efflux pumps. Efflux pumps are transmembrane proteins that can extrude antimicrobials from bacterial cytoplasm. Efflux pumps recognize a wide range of compounds including antibiotics, antiseptics, pesticides, and hormones. The upregulation of efflux pump genes in response to increasing concentrations of substrates has shown to increase resistance to toxic substrates. We hypothesize that efflux pump substrates other than antimicrobials, such as steroid hormones, will also induce the expression of efflux pump genes and consequently increase resistance …

Parks, Recreation, And Opportunities: A Case Study Of East Bay Regional Park District's Climate Change Mitigation And Adaptation Strategies, Brett Walsh

Experience Industry Management

In the face of a global climate emergency, community parks & recreation and land management agencies are beginning to adopt a critical role in the fight against climate change. This study gathered information on efforts around the world and created a case study guide to examine the climate change mitigation and adaptation strategies of East Bay Regional Park District in the San Francisco Bay Area of California in the United States. The study was conducted in hopes of shedding light on how small agencies can be proactive and contribute to larger efforts, creating greater regional impacts.

A Transfer Students Guide To Becoming A Pca, Zack J. Krueger

Agricultural Education and Communication

A transfer students guide to becoming a PCA through the AGED department as opposed to AEPS. Agricultural science majors. Classes shown and listed cooperate with the California Department of Pesticide Regulations required course list necessary to take the Pest Control Advisers exam.

Heavy Metal Dissolution Mechanisms From Electrical Industrial Sludge, Viraj Gunarathne, Anushka Upamali Rajapaksha, Meththika Vithanage, Nadeesh Adassooriya, Asitha Cooray, Sudantha Liyanage, Bandunee Athapattu, Nishanta Rajakaruna, Avanthi Deshani Igalavithana, Deyi Hou, Daniel S. Alessi, Yong Sik Ok

Biological Sciences

In this paper, we investigate the release of heavy metals from sludge produced from an electrical industry using both organic and inorganic acids. Single and sequential extractions were conducted to assess heavy metals in different phases of the sludge. Metal release from sludge was investigated in the presence of three inorganic acids (nitric, sulfuric, and phosphoric) and three organic acids (acetic, malic, and citric) at concentrations ranging from 0.1 to 2.0 mol L−1. Sequential extraction indicated the presence of Cu primarily in the carbonate fraction, Pb in the residual fraction, and Ni in the FeMn oxide fraction. The cumulative release …

Blood Glucose Predictor, Jessica Patterson

Electrical Engineering

For my senior project, I perform data analysis using statistical methods to determine body metrics that correlate with blood glucose levels. Working with Dr. Tina Smilkstein, I take repeat measurements from 6 different volunteers to establish trends in bodily metric data. The data taken includes weight, body fat, pulse rate, VO2, blood glucose, blood pressure, hours slept, and quality of sleep. Using these values, I use the program MiniTab to view results.

A few examples of correlations with blood glucose found in this project are:

• Systolic blood pressure for females had a regression line of 124.0 -0.3366*Blood Pressure. This indicates …

Wine History Pavilion: Flow, Isaac J. Cameron

Architectural Engineering

The Wine History Pavilion has been designed using Integrated Project Delivery, a delivery approach that has been gaining popularity that involves the participation of multiple disciplines at every stage of design. For this group, that entails the participation of students from the departments of Architecture, Architectural Engineering, and Construction Management working in tandem to handle every facet of the design.

The goal of this project is to design a pavilion for the Wine History Project, who needed a display space to house a variety of exhibits showcasing artifacts relevant to the history of wine. The pavilion will originally be installed …

Do Elevation And Aspect Predict Functional Trait Values In Alpine Plant Populations?, Karen Analiesa Bach

Biological Sciences

Alpine regions are among the hardest hit by global climate change, experiencing rising temperatures at a faster rate than lowland areas. Such fast rates of warming are predicted to cause rapid changes in alpine plant communities, including shifts in plant functional traits along elevation gradients. To investigate these effects within species, we examined the relationship between elevation, aspect, and functional traits of three common alpine plants in California; the shrub Holodiscus discolor var. microphyllus, the forb Calyptridium umbellatum, and the grass Trisetum spicatum. Functional traits examined included plant height, plant width, and Specific Leaf Area (SLA). These …

The Water Table, Soil Moisture And Evapotranspiration Conditions Following The Removal Of Conifers From Two Encroached Meadows, Tyler J. Davis

Master's Theses

Montane meadows provide essential habitat for a variety of unique species and important ecosystem services in the western United States. Although important, meadows have experienced increased rates of conifer encroachment due to climate change, fire suppression and grazing. To combat meadow degradation from conifer encroachment, land managers have employed various restoration strategies one of which is conifer removal. Multiple studies have investigated the relationship between meadow hydrology and vegetation; however, few have assessed the effect of conifer removal on meadow groundwater. The goal of this study is to determine if the removal of conifers from an encroached meadow has an …

Evaluation Of Pheromone Mating Disruption For California Red Scale Control In Commercial California Citrus, Joel Timothy Leonard

Master's Theses

California red scale (CRS), Aonidella aurantii, is an increasingly injurious insect pest for the California citrus industry due to insecticide resistance, changing weather patterns, and shifting trade regulations. The presence of the insect on fruit, damages the rind of the fruit and high populations can cause dieback of branches as well as lower yields. Pheromone mating disruption of CRS has the potential to alleviate population control concerns and reduce insecticide use. The efficacy of the pheromone mating disruption technique for pest management of CRS was determined using the products CheckMate® CRS and Semios CRS Plus. CheckMate® CRS was evaluated …

Single-Cell Impedance Spectroscopy, David Paul Lange

Master's Theses

Impedance spectroscopy (IS) is an important tool for cell detection and characterization in medical and food safety applications. In this thesis, the Cal Poly Biofluidics Lab’s impedance spectroscopy system was re-evaluated and optimized for single-cell impedance spectroscopy. To evaluate the IS system, an impedance spectroscopy bioMEMS chip was fabricated in the Cal Poly Microfabcrication lab, software was developed to run IS experiments, and studies were run to validate the system. To explore IS optimization, Maxwell’s mixture theorem and the Schwartz-Christoffel transform were used to calculate an analytic impedance solution to the co-planar electrode system,a novel volume fraction to account for …

Executive Committee - Meeting Minutes, 11/05/2019, Academic Senate Executive Committee

Academic Senate Minutes

No abstract provided.

Executive Committee - Agenda, 11/05/2019, Academic Senate

Academic Senate Agendas

No abstract provided.

Losing Our Minds To Madness: Paradigm Changes In Western European Perceptions Of Mental Illness, James Michael Cecil

History

Academia and scholarship of the 20th-century bred a renewed interest in mental illness throughout history. Despite an increase in the literature within the discourse surrounding "madness," scholars have generally failed to understand how and why Western European societies have viewed mental illness in various ways throughout recorded history. This paper argues that there remains an inherent, human desire to reject anything different from humanity, particularly mental illness, which is nearly impossible to fully comprehend. This is especially true in the case of how societies have institutionalized, punished, and subjugated the "mad" individual.

The Democratization And Development Of Cell-Free Protein Synthesis, Max Z. Levine

Master's Theses

Cell-free protein synthesis (CFPS) using crude lysates has developed into a robust platform technology over the last 60 years to express numerous types of recombinant proteins. The open-nature, elimination of reliance on cell viability, and focus of all energy towards production of the protein of interest represent substantial advantages of CFPS over in vivo protein expression methods. CFPS has provided new opportunities across a series of research fields that include metabolic engineering, therapeutic and vaccine development, education, biosensors, and many more. In recent years, optimizations of CFPS have even allowed the platform to reach the industrial level of protein production. …

Effects Of Intertidal Position On Metabolism And Behavior In The Acorn Barnacle, Balanus Glandula, Kali Horn

Master's Theses

The intertidal zone is characterized by persistent, tidally-driven fluctuations in both abiotic (e.g., temperature, [O₂], salinity) and biotic (e.g., food availability, predation) conditions, which makes this a very physiologically challenging habitat for resident organisms. The magnitude and degree of variability of these environmental stressors differs between intertidal zones, with the most extreme physiological stress often being experienced by organisms in the high intertidal. Given that many of the fluctuating conditions in this environment are primary drivers of metabolic rate (e.g., temperature, [O₂], food availability), we hypothesized that sessile conspecifics residing in different tidal zones would exhibit …

Spatial And Temporal Variation Of Offshore Wind Power And Its Values Along The Central California Coast, Yi-Hui Wang, Ryan K. Walter, Crow White, Matthew D. Kehrli, Stephen F. Hamilton, Patrick H. Soper, Benjamin I. Ruttenberg

Physics

The analysis of the spatiotemporal variability of wind power remains limited during the planning stage of an offshore wind farm. This study provides a framework to investigate how offshore wind power varies along the Central California Coast over diurnal and seasonal time scales, which is critical for reliability and functionality of the grid system. We find that offshore wind power in this region peaks during evening hours across all seasons and maximizes in spring and summer. The timing of peak offshore wind power production better aligns with that of peak demand across California than solar and land-based wind power production, …

Executive Committee - Agenda, 10/01/2019, Academic Senate

Academic Senate Agendas

No abstract provided.

Cal Poly Frankenreads: An All-Day Public Reading Of Mary Shelly’S Frankenstein, Robert E. Kennedy Library

Creative Works

Celebrating the 200th anniversary of Mary Shelly’s Frankenstein, the Cal Poly English Department and Kennedy Library organized a series of interdisciplinary events including FrankenReads, an all-day public reading of the novel. Spanning twelve hours, members of the Cal Poly community from all colleges participated in the celebration by volunteering to read portions of Frankenstein; or, The Modern Prometheus.

This catalog is based on the celebration of events “FrankenFall” which took place on October 31, 2018 at the Robert E. Kennedy Library.

A Study On The Simultaneous Nitrification And Denitrification Process Of A Membrane Aerated Bioreactor Augmented By Biowish Aqua, Gavrielle Orman

Master's Theses

Nitrogen pollution is a growing problem that is detrimental to the environment and the economy. Traditional treatment of nitrogen is a multi-stage process, expensive, operationally intensive, and requires large land areas. This research studied the effects of BiOWiSH® Aqua (Aqua), a biological enhancement product, on the simultaneous nitrification and denitrification process in a membrane aerated bioreactor (MABR) to determine if it is a feasible application for wastewater treatment. The MABR used during experimentation was a small-scale batch reactor with a continuous flow of air through a silicone membrane.

The effect of carbon source and concentration on nitrogen removal rates and …

Executive Committee - Meeting Minutes, 9/24/2019, Academic Senate Executive Committee

Academic Senate Minutes

No abstract provided.

Executive Committee - Agenda, 9/24/2019, Academic Senate

Academic Senate Agendas

No abstract provided.

Developing Process Variables Necessary To Operate Simulacrum: The Lcls Accelerator Simulator, Mirian G. Juan Estrella

STAR Program Research Presentations

The Linac Coherent Light Source (LCLS) is a free electron laser that is located at SLAC National Accelerator Laboratory. It fires 120 pulses per second, creating x-ray snapshots of materials at the atomic and molecular level as it undergoes any changes or processes. This resulted in many scientific discoveries in chemistry, biology, energy science, and technology. Now, there is a new vision at SLAC regarding LCLS: to develop Simulacrum. Simulacrum is a system that simulates LCLS and its control system. Within Simulacrum there exits services that contain process variables that measure specific parts of a device on LCLS, which communicate …

Santa Rosa Island Restoration Study: Fog Fences Vs. No Fences, Aimee Newell, Stephen Bednar, Kathryn Mceachern

STAR Program Research Presentations

Santa Rosa Island, Channel Islands National Park, was grazed by cattle, sheep, pigs, elk, and deer for 154 years, degrading the island’s vegetation and causing erosion. In 1998, the livestock were removed, and in 2011 the remaining big game animals were removed, allowing ecosystem recovery to begin. This study evaluated growth of two native species planted in 2016, at the cloud forest restoration site on the Soledad Ridge. Baccharis pilularis (coyote brush) was planted as nursery stock from 2-inch pots, while Quercus pacifica (island scrub oak) was planted as sprouted acorns. The plants were watered with drip irrigation 2016-2018, …

An Application Of Sliding Mode Control To Model-Based Reinforcement Learning, Aaron Thomas Parisi

Master's Theses

The state-of-art model-free reinforcement learning algorithms can generate admissible controls for complicated systems with no prior knowledge of the system dynamics, so long as sufficient (oftentimes millions) of samples are available from the environ- ment. On the other hand, model-based reinforcement learning approaches seek to leverage known optimal or robust control to reinforcement learning tasks by mod- elling the system dynamics and applying well established control algorithms to the system model. Sliding-mode controllers are robust to system disturbance and modelling errors, and have been widely used for high-order nonlinear system control. This thesis studies the application of sliding mode control …

Fucus Distichus: Investigating Humidity And Temperature Between Tides, Jason Thomas, Andrew Chang, Julia Blum, Chela Zabin

STAR Program Research Presentations

Environmental elements such as changing rapidly changing temperature, prolonged periods of low to no water exposure, desiccation, predation and increased wave action can influence the diversity of microhabitats that inhabit the littoral zones. When making observations of various shorelines, specifically the physical conditions of the surrounding marine vegetation (i.e. Fucus distichus), an inquiry is made as to the role of Fucus in the amelioration of stressors on the marine habitat during low tide (MLLW), however, due to time considerations, temperature and humidity are the focus of this study. Using temperature and humidity probes, monitoring data shows that Fucus provides …

The Search Of Ancient Life Through The Use Of Geologic Samples, Amber Paturzo

STAR Program Research Presentations

The Astrobiogeochemistry Laboratory (abcLab) is interested in exploring molecular and mineralogical biosignatures in ancient and recent Earth samples to provide evidence of early signs of life. Currently, in the abcLab, scientists are utilizing a lipid biomarker extraction and analysis method to remove organic material from geologic samples taken from several sites around the world including Australia, the United States and West Wales. This study focuses primarily on samples collected from the Mochras borehole on the Cardigan Bay Basin, West Wales, dating back to the Early Jurassic to identify biomarkers such as hopanes (derived from prokaryotes) and steranes (derived from eukaryotes) …

Distance And Its Effect On Betta Aggression, Sydnie Hanson

STAR Program Research Presentations

Betta Splendens are typically known to act very aggressive towards one another, especially between two males. These acts of aggression are shown through flaring of ones fins, chasing and striking opposing Bettas, biting, etc. This experiment shows how distance affects how aggressive male Bettas are with one another

Glycolic Acid Utilization In Two Species Of Marine Bacteria, Erik S. Timsak

STAR Program Research Presentations

Interactions between bacteria and phytoplankton are crucial for the cycling of organic matter in marine environments. Around 50% of organic carbon taken up by marine bacteria is converted into inorganic carbon. The uptake of organic carbon by marine bacteria exuded from phytoplankton is a key factor in regulating the marine carbon cycle. One such molecule that is exuded by phytoplankton and then uptaken by marine bacteria is called glycolate - the anion of glycolic acid, a two caron molecule. Glycolate is exuded by phytoplankton during photorespiration and 10-50% of dissolved organic carbon in marine environments is comprised of glycolate. Additionally, …

Planetary Protection Requirements For Robotic And Human Missions, Karen Duenas

STAR Program Research Presentations

Planetary Protection involves the development and implementation of policies and requirements to protect solar system bodies from forward and backward contamination during human or robotic space missions. As stipulated by the United Nations Outer Space Treaty (Article IX) and interpreted by the Committee on Space Research, over 100 nations abide by the treaty and its objectives. Since the beginning of the space age, NASA has complied with planetary protection requirements across more than 250 robotic and human missions, especially those involving Mars, which is a planet with potentially habitable conditions for microbial life. A sample of past, current, and future …

Abundance And Community Composition Of Invasive Intertidal Watersipora On The San Francisco Bay Area Outer Coast, Kevin Myron, Meredtyh Duncan, Jaden Stone, C. Sarah Cohen

STAR Program Research Presentations

Invasive species can lead to serious ecological changes. The San Francisco Bay area is one of the most invaded areas in the world due to the commercial shipping industry and recreational water vessels. While the intertidal is not widely invaded, the harbors and docks are. One exception to this is the invasive, colonial filter-feeding bryozoan Watersipora, which has been found at various rocky outer coast sites. Further investigation into how the sites are invaded by this organism and their impact on native species must be carried out. In this project, at four intertidal sites around the SF Bay outflow, we …