Digital Commons Network^™

Spatial And Temporal Variation In Growth Rate Of Blue Rockfish (Sebastes Mystinus) In Nearshore Central California Determined Using A Physiological Biomarker, Ellie M. Brauer

Master's Theses

Identifying areas of high fish population productivity is crucial for protecting habitats essential to fish growth and reproduction and, ultimately, for achieving sustainable fisheries. Historically, evaluations of habitat quality have relied heavily on linking spatial variation in fish abundance to environmental parameters such as substrate category, depth, or bathymetry. That approach, however, assumes that areas of high fish abundance best support growth and reproduction of a species and thus may fail to detect spatial or temporal variation in population attributes, such as somatic growth rate, which can be central to recruitment success and survival. In this study, we employed a …

Optimization Of A Novel Nipam-Based Thermoresponsive Copolymer For Intramuscular Injection As A Myoblast Delivery Vehicle To Combat Peripheral Artery Occlusive Disease, Quentin R. Klueter

Master's Theses

There is a need for a minimally invasive delivery method to enable cell therapies to combat peripheral artery occlusive disease (PAOD) in end stage patients. Myoblasts show promise as a cell mediated therapy but warrant an improved delivery method to increase cell retention in the region of interest because of their adherent nature, relative to previously used BM-MNC’s that are non-adherent. Contemporary issues with achieving successful cell therapies of vasculature can be mainly characterized by the lack of clinical translation from promising animal studies and absence of cell delivery scaffolding. Naturally, polymers have been widely experimented with as grafts to …

Impact Of Primary Myoblasts On Macrophage Polarization In-Vitro, Olivia Welch

Master's Theses

Peripheral artery disease (PAD) is characterized by the development of atherosclerotic plaques on arterial walls, leading to the narrowing of blood vessels, resulting in ischemia in the downstream tissue. In the United States, 12% of the adult population is affected by PAD and its related symptoms. Current surgical revascularization techniques can be effective in part of the patient population, but there is a need for other options. Alternatively, collateral blood vessels, or natural bypass arteries, enlarge to increase blood flow to the ischemic tissue in a process called arteriogenesis, which has been studied as a therapeutic option. Cell-based therapies, such …

Santa Margarita Watershed Sanitary Survey Update For 2021, Timothy Chen

Master of Science in Environmental Sciences and Management Projects

The Watershed Sanitary Survey update evaluates the water quality of a groundwater aquifer utilized by the community of Santa Margarita as a municipal water source through Well No. 4. It was last conducted in 2011. The Watershed Sanitary Survey is a regulatory document for the compliance of Santa Margarita’s water system with the Surface Water Treatment Rule (SWTR), aimed at protecting the public from any sources of drinking water contamination. The unprecedented level of drought occurring over the last five years could spell unknown changes to the water quality of the aquifer, possibly increasing the concentration of pollutants. This poses …

The Importance Of Health Literacy: A Student-Led Workshop On Lay Communication, Sarah Jean Kamp, Jafra D. Thomas

Kinesiology and Public Health

The purpose of this experiential senior project workshop was to advance the knowledge and practice of health communication by (a) delivering a training workshop to Cal Poly undergraduate students and (b) by exploring the relationship between health literacy and effective communication through completion of a rapid review of the literature. The reviewed literature served the purpose of helping the student further design the workshop to elicit a foundational understanding of the elements of effective communication of health information as well as the history and evolution of health literacy as a concept. The workshop revised and delivered by the student was …

Graduate Internship In Agricultural Education Report & Project, Anthony Farao

Agricultural Education: Graduate Internship Reports

This internship and project report includes the documentation required in meeting the quality criteria for secondary programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Wasco High School, conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Wasco High School, the agriculture program, and the community.

Aged 539 Internship Report - Lahainaluna High School,, Caitlyn R. Madrid

Agricultural Education: Graduate Internship Reports

This internship and project report includes documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for Lahainaluna High School agriculture programs. The supporting material includes information to begin a floral subscription program as well as explore the cultural diversity and inclusion of Hawaiian cultures and Hawaiian agriculture.

Aged 539 Graduate Internship Report - Everett Alvarez High School, Miranda Pawling

Agricultural Education: Graduate Internship Reports

This internship and project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Everett Alvarez High School, the agriculture program and the community.

Bone And Cartilage Compression Simulator, Karoline M. Wucherer, Benjamin A. Parmentier, Thomasina E. Hinkle

Biomedical Engineering

A device was developed that delivers mechanical loads to bone and soft tissue samples under physiological conditions to aid in the research of tissue engineering bone and cartilage. To begin the design process, a Network Diagram and Gantt Chart were produced to create a general timeline for the project to follow. This allowed us to measure our progress and determine what effects (if any) delays could have on our project.

Live Cell Imaging Gas Control, Elsa M. Bean, Brady C. Berg, Abby A. Jens

Biomedical Engineering

The following document outlines the entire process for designing and manufacturing a Live Cell Imaging Gas Control subsystem in tandem with the Heated Stage subsystem to make a CO₂ and temperature-controlled chamber for use with the Olympus IX73P2F Microscope. It highlights the current literature and patents surrounding live cell imaging and touches on the existing live cell imaging setups currently being used by other entities. Following this, it defines the scope of the project, key customer requirements, and engineering specifications to ensure the design meets all the needs of its users. This document includes project planning steps to establish …

Verification Of A Digital Microfluidics Platform, Karanpartap Singh

Electrical Engineering

The electrowetting effect describes the change in contact angle between a solid surface and electrolyte in response to an applied electric potential difference. Given a planar array of individually-actuated electrodes, electrowetting can be used to transport, mix, and separate picoliter to microliter-sized droplets of liquid on a dielectric layer. Applications of this phenomenon range from lab-on-a-chip and other microfluidic devices to liquid lenses capable of altering their topology and focus within milliseconds.

This project extends prior work simulating the dependence of droplet velocity on actuation voltage and demonstrates observed physics on a physical platform. The simulation portion of this project …

Aged 539 Graduate Internship Report- Washington Union High School, Manuel Torres Jr.

Agricultural Education: Graduate Internship Reports

This internship and project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Washington Union High School, the agriculture program and the community.

Minivette Integrated Sample Prep Funnel For Cepheid Cartridges, Hanna N. Blumer, Sophia Arias, Camille Porciuncula, Ben Runkle

Biomedical Engineering

This report covers the redesign of the Sample Prep Funnel for Cepheid’s engineering team. The Sample Prep Funnel, responsible for relaying liquid from the sample collection device to the testing cartridge, experiences fluid loss that causes invalid test results. Our group's task was to redesign this funnel to minimize fluid loss and, overall, increase efficiency and effectiveness.

Lytic Bacteriophages And Lactic Acid As Processing Aids Against Salmonella Spp. And Escherichia Coli O157:H7 On Marinated And Tenderized Pork Loins, Sherita Li

Master's Theses

Within the last decade, pork consumption has steadily increased and continues to be the most consumed meat globally. However, pathogenic bacterial strains resistant to antibiotics have also been increasingly found in pig farms, animals, and the environment. Bacterial food poisoning cases due to Salmonella spp. and Escherichia coli (E. coli) O157:H7 appear to be linked with a variety of pork products. The meat industry has recognized that research is needed to combat the multi-drug resistance in foodborne pathogens with alternative methods of control. This study evaluated the effects of both E. coli- and Salmonella-specific lytic bacteriophages and …

Fire Effects In Montane Meadows, Rosie Deak

Master's Theses

The impact of forest fires on downstream meadow communities across California is of great ecological interest, as meadows are an important source of biodiversity in this region. Over a century of fire suppression has led to increased forest stand densities, which in turn has resulted in less water availability due to increased transpiration of densely growing trees. This potentially has left less available water for downstream plant communities in meadows. If true, then high mortality wildfires in surrounding forest are predicted to lead to an increase in available downstream moisture where obligate and facultative-wetland taxa increase and dry-adapted upland taxa …

Strategies To Promote Responsive Bottle-Feeding In Wic Predict Less Frequent Use Of Food To Soothe And Healthier Weight Status For Infants With Negative Temperaments, Alison K. Ventura, Shannon E. Whaley

Kinesiology and Public Health

Background: Infants with greater temperamental negative affectivity are at higher risk for overfeeding and excess weight gain.

Objective: To examine whether strategies to promote responsive bottle-feeding within WIC promoted healthier maternal feeding practices and infant weight status among infants with greater negative affectivity.

Methods: Secondary analysis of data from a matched-pair cluster randomized trial. Policy, systems and environmental change (PSE) strategies to promote responsive bottle-feeding were implemented at three WIC clinics; these clinics were compared with three matched control clinics. Linear mixed models tested whether infant negative affectivity interacted with PSE strategies to predict feeding and weight outcomes when infants …

Academic Senate - Agenda, 2/22/2022, Academic Senate

Academic Senate Agendas

No abstract provided.

Integration Of Electrical Impedance Spectroscopy For Multichannel Cell Culture Measurement, Conard Chan

Master's Theses

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY (EIS) has been widely used to study the electrical properties of biological material due to its non-invasive nature and experimental reliability. However, most of the precision impedance analyzers used in EIS only provide single- or two-channel measurements which are inadequate for larger-scale multiplexed measurements, such as those found in modern microfluidic cell culture experiments. The Biomedical Microsystems Laboratory has developed a 16-channel cell culture platform with integrated electrode arrays for monitoring cell growth and electrical properties (i.e., the so-called “electrical phenotype”). In this paper, a system consisting of a 16-channel solid-state analog multiplexer (MUX)paired with a low-cost, …

Effects Of Intertidal Position On The Capacity For Anaerobic Metabolism And Thermal Stress Response In The Common Acorn Barnacle, Balanus Glandula, Kyra Anderson

Master's Theses

Intertidal habitats are characterized by dynamic, tidally-driven fluctuations in abiotic and biotic factors. Many of the environmental stressors that vary across the intertidal (e.g., temperature, oxygen, food availability, predation pressure) are strong drivers of metabolic rate in ectotherms. As such, we predicted that there may be pronounced differences in the metabolic and stress physiology of conspecific sessile invertebrates occupying at different relative tidal heights. The common acorn barnacle Balanus glandula represents an ideal model organism in which to investigate the possibility of tidal height-dependent physiological differences, owing to their wide distribution in the intertidal zone and their eurytolerant nature. In …

Hierarchical Classification Of Deep-Sea Videos, Lubomir Stanchev, Hanson Egbert

Computer Science and Software Engineering

This research is based on previous research that proposes the use of AI algorithms to automatically annotate deep-sea videos [1]. Regardless of whether a deep-sea video is annotated automatically or by a human expert, some frames are difficult to annotate because one or more objects may be ambiguous to interpret. For example, the Funiculina and Umbellula Lindahli are two types of sea pens that are difficult to distinguish. Similarly, an object may be easily identified as a fish, but the type of fish may be difficult to determine. For these types of circumstances, we propose a hierarchical classification algorithm that …

Synthetic Design And Anisotropic Conductivities Of Polymeric Mixed Ion- Electron Conductors, Nika Bondar, Abigail Slimp, Patrick Gruoner, Jason Lin

Baker/Koob Endowments Awarded Projects

Our project documented, in extensive detail, the liquid crystalline (LC) assembly pathway from solution to solid state in the supramolecular complexes of the mixed ionic-electronic conducting polymers (MIECPs) prepared from carboxylated poly(3-alkylthiophene) and ionic liquid surfactant. Using UV-visible spectroscopy we have shown that the complexation is accompanied by the surfactochromism showing colorimetric response and conformational transitions as a function of the IL surfactant mole ratio. Additionally, we have demonstrated via Polarized Optical Microscopy (POM), that the equimolar solution exhibits rodlike conformation and its hydrogel spontaneously forms a 2D smectic LC mesophase with a lamellar periodicity. Defect-free LC domains were produced …

Writing Arguments In Stem, Jason Peters, Jennifer Bates, Erin Martin-Elston, Sadie Johann, Rebekah Maples, Anne Regan, Morgan White

OER Course Materials

A team of faculty at California Polytechnic State University, San Luis Obispo, curated the contents to support instructors teaching first-year courses in critical thinking and communication.

The Question Of Veganism, The Dangers Of Moral Extensionism, And A Pragmatist Ecofeminist Alternative, Erin Mckenna

Between the Species

In this paper I argue that the framework of moral extensionism relies on human exceptionalism and human centeredness. I discuss the dangers of human exceptionalism and human centeredness using the work of ecofeminist philosophers Val Plumwood, Carol Adams, Lori Gruen, A. Breeze Harper, and Lisa Kemmerer. These ecofeminists each articulate alternative approaches to human relations with other animal beings. There are tensions among these alternatives, though, and I use a pragmatist perspective to interrogate their different positions on how other animal beings should or should not figure into the diets of human beings. I will argue that we need a …

Extending The Impairment Argument To Sentient Non-Human Animals, Christopher A. Bobier

Between the Species

I defend a new argument against raising and killing sentient non-human animals for food: It is immoral to non-lethally impair sentient non-human animals for pleasure, and since raising and killing sentient animals for gustatory pleasure impairs them to a much greater degree, that also is immoral. This argument is structurally analogous to Perry Hendricks’s impairment argument for the immorality of abortion. Proponents of the anti-abortion argument have to be, on grounds of moral consistency, proponents of the anti-meat eating argument: the very same considerations they appeal to to justify their anti-abortion impairment argument apply to the impairment argument against raising …

Understory Vegetation Composition And Small Mammal Abundance In An Exotic Eucalyptus Forest Vs Adjacent Native Habitat, Kelly Mcfadden

Master of Science in Environmental Sciences and Management Projects

None provided

Evaluation Of Heat Tolerant Cauliflower Varieties On California’S Central Coast, Lauren Booke

Master of Science in Environmental Sciences and Management Projects

Heat tolerant trials of vegetable crops will help to improve food security when it becomes affected by rising temperatures due to climate change. By having heat tolerant vegetable crops, we can ensure the well-being of individuals in our society—nutritionally, economically, and socially. California is responsible for 90% of the cauliflower production in the United States. This research aims to determine the overall productivity of three heat tolerant cauliflower varieties (Bishop, Mardi, Flame Star) during the summer months (July- September) on California’s central coast. Stomatal conductance and chlorophyll fluorescence were measured throughout the growing cycle to evaluate plant stress and photosynthetic …

Apache Science Team, Denise L. Jameson

Agricultural Education: Graduate Internship Reports

Sanger High school has the unique opportunity to offer a wide array of classes and pathways for students to take so that they can meet their educational goals of graduation the move on to higher education or starting a career right out of high school. Students have all these opportunities and benefits to have a strong start out of high school, but ultimately students are unaware of what jobs are related to those choices or pathways that they have decided to take part in. Apache Science Team allows for students to experience and learn about many different careers and opportunities …

Aged 539 Graduate Internship Report - Grace M. Davis Agriculture Department, Joshua S. Aguilar

Agricultural Education: Graduate Internship Reports

This internship and project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The supporting material includes information to receive state and local funding, outlines the goals and objectives of the program, along with an overview of Grace M. Davis High School, the agriculture program, and the community.

Rigid Aggregation Of Inclusions Embedded In Quasi 2d Fluids, Natalie Xochitl Ryan

Physics

Diffusion is a transport process common in several biological systems. In this process particles of different species mix together through random (stochastic) motion at molecular length scales. Diffusion in fluids is unique as the coupling of the flow and fluid have been found to produce giant concentration fluctuations. The molecular length scale of these concentration fluctuations are magnitudes larger than the movement of the particles themselves, earning them the title “giant”. The diffusion of particles in bio-membranes displays a combination of 2D and 3D hydrodynamic properties; the movements of the particles are restricted to the plane of the membrane and …

Map-Gan: Unsupervised Learning Of Inverse Problems, Brandon S. Campanella

Master's Theses

In this paper we outline a novel method for training a generative adversarial network based denoising model from an exclusively corrupted and unpaired dataset of images. Our model can learn without clean data or corrupted image pairs, and instead only requires that the noise distribution is able to be expressed analytically and that the noise at each pixel is independent. We utilize maximum a posteriori estimation as the underlying solution framework, optimizing over the analytically expressed noise generating distribution as the likelihood and employ the GAN as the prior. We then evaluate our method on several popular datasets of varying …