Digital Commons Network^™

Conversion Of Greenhouse Gasses Into Energy, Sarah C. Rippe

Social Sciences

No abstract provided.

An Analysis Of Eliminating Electroosmotic Flow In A Microfluidic Pdms Chip, Cecile D. Redington

Master's Theses

The goal of this project is to eliminate electroosmotic flow (EOF) in a microfluidic chip. EOF is a naturally occurring phenomenon at the fluid-surface interface in microfluidic chips when an electric field is applied across the fluid. When isoelectric focusing (IEF) is carried out to separate proteins based on their surface charge, the analytes must remain in the separation chamber, and not migrate to adjacent features in the microfluidic chip, which happens with EOF.

For this project, a microfluidic chip was designed and commissioned to be photolithographically transferred onto a Si wafer. A PDMS component was then casted on the …

Cal Poly To Help Launch New Model Of Online Learning, Public Affairs Office

Press Releases

No abstract provided.

Traversing Swanton Road, 13th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

Synthesis And Evaluation Of Thermally-Responsive Coatings Based Upon Diels–Alder Chemistry And Renewable Materials, Dahlia N. Amato, Gregory A. Strange, John P. Swanson, Anton D. Chavez, Suzanne E. Roy, Kim L. Varney, Craig A. Machado, Douglas V. Amato, Philip Costanzo

Chemistry and Biochemistry

A soybean based coating with thermally responsive Diels–Alder linkages has been prepared following an automotive 2-component formulation. The resulting coatings displayed the capability to be healed following physical deformation by a thermal stimulus, and such a material has significant potential for end users. Various curing agents were employed, and resulted in variation of scratch resistance and re-healablity. Different thermally responsive soybean resins were synthesized to have varying amounts reversible and nonreversible linkages when incorporated into the coating. Additionally, different isocyanates were added at differing ratios of NCO:OH in search of the optimum coating. It was found through the analysis of …

Towards Three-Dimensional Underwater Mapping Without Odometry, Alistair Dobke, Joshua Vasquez, Lauren Lieu, Ben Chasnov, Christopher Clark, Ian Dunn, Zoë J. Wood, Timothy Gambin

Computer Science and Software Engineering

This paper presents a method for the creation of three-dimensional maps of underwater cisterns and wells using a submersible robot equipped with two scanning sonars and a compass. Previous work in this area utilized a particle filter to perform offline simultaneous localization and mapping (SLAM) in two dimensions using a single sonar [11]. This work utilizes scan matching and incorporates an additional sonar that scans in a perpendicular plane. Given a set of overlapping horizontal and vertical sonar scans, an algorithm was implemented to map underwater chambers by matching sets of scans using a weighted iterative closest point (ICP) method. …

Bead Based Multiplexing For The Simultaneous Detection Of Salmonella Enterica, Listeria Monocytogenes, Escherichia Coli, Francisco Fernandez, Karamveer Singh, Sonam Sahni, Alejandro Calderon-Urrea

STAR Program Research Presentations

With the ever increasing population of Earth, it becomes crucial that the integrity of our food supply is maintained; outbreaks of food borne pathogens can affect people on a much grander scale if they this is not so. This research project set out to test whether it was possible to test for multiple pathogens in a single sample via a single assay, and if that was possible, what are the limits of detection (LOD) of this assay? The assay involved the use of magnetic micro-beads coupled with predesigned primers and biotinylated TaqMan probes that would report the presence of proteins …

Zooplankton Trophic Ecology In The San Francisco Estuary During Summer As Determined By Stable Isotope Analysis, Kyla Bradylong, Steven Westbrook, Julien Moderan, Wim Kimmerer

STAR Program Research Presentations

Declines in the abundance of several pelagic fish species in the upper San Francisco Estuary have prompted investigation into food web interactions within the estuary and delta (the lower Sacramento and San Joaquin Rivers). This area is characterized by low primary production and pelagic food webs much longer and reticulated than previously thought, implying low efficiency in the energy transfers from primary producers to planktivorous fish. We determined the carbon and nitrogen stable isotope (SI) composition of zooplankton samples collected monthly between June 2012 and February 2013 at eight stations along the salinity gradient. As consumers SI composition reflects that …

Big Babies, Big Mammas?: Relationship Of Leatherback Hatchling Size And Mother Size, Shane Morales, Violet Campbell, Kelly Stewart

STAR Program Research Presentations

Individual leatherback hatchlings vary in size when compared to individuals from other nests, as well as individuals from the same nest. It is thought that many factors affect hatchling size but that one of the most influential factors is maternal size. Of all the aspects of a mother which could affect hatchling size, evidence for question concerning influence of mother size is determinable within the field using minimal tools and basic statistical analysis. If a direct correlation exists between mother size and hatchling size then the claim can be made that larger mothers produce larger offspring while smaller mothers produce …

Abundance And Distribution Of Microplankton In The San Francisco Estuary, Carrie Ann Sharitt, Lindsay Sullivan, Wim Kimmerer

STAR Program Research Presentations

Microplankton are a diverse group of planktonic organisms ranging from 0.02 to 0.2 millimeters. Since the group is defined solely by size, it spans numerous taxonomic groups, including both heterotrophs and autotrophs. Microplankton are abundant in all aquatic ecosystems and are important prey for many organism, including bivalves, crustaceans, and fish. Little is known about the microplankton community in the San Francisco Estuary, and information on their abundance and distribution will help scientists better understand their role in local foodwebs. This research quantified the abundance and distribution of microplankton in the San Francisco Estuary from 2010 to 2012. Microplankton were …

Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli

STAR Program Research Presentations

In deep space missions, maintaining life support is of the utmost priority. In such a closed system, human waste must be stored and treated. Simulated feces (ersatz) will be inoculated with microbes representing normal fecal flora, mixed with urine brine and shredded refuse of products typically used in space missions. Composting methods often use alternating layers of waste with scraps of carboniferous materials (finely shredded refuse). By preparing membrane bags with a homogenized ersatz and carboniferous refuse mixture and membrane bags with alternating layers of ersatz and carboniferous refuse, it may be possible to monitor anaerobic thermophillic digestion of the …

Vertical Beam Size Measurements In The Spear3 Accelerator At Slac, Natalie Ann Larosa, Jeff Corbett

STAR Program Research Presentations

Synchrotron radiation is a powerful tool used in many fields of science ranging from materials characterization to structural biology. Each year thousands of scientists travel to SLAC to use high-resolution x-rays emitted from a relativistic electron beam circulating in the SPEAR3 synchrotron light source. To characterize the beam size in SPEAR3, we constructed a visible-light interferometer capable of measuring the 22um vertical beam size. The interferometer is located 17m away from the source point and consists of two vertically separated slits. Visible light emitted from the synchrotron passes through the two slits and interferes on a CCD camera to produce …

Spectroscopic Study Of Uranium (Vi) Reduction By Plant Biomass, Margaret C. Murphy, John Bargar, Noémie Janot

STAR Program Research Presentations

Uranium is a common and problematic groundwater contaminant at Department of Energy legacy sites. At the former uranium ore processing plant at Rifle, Colorado, sediments rich in decayed plant biomass contain large concentrations of uranium that are slowly being released back to the aquifer. To simulate the reaction that occurs in organic rich sediments of the Rifle aquifer, biomass was incubated in U (VI) bearing groundwater. Carbon X-ray absorption spectroscopy (XAS) was then used to measure if uranium was bound to the biomass. When uranium binds to biomass the peaks in the C XAS spectra will change shape. Uranium L …

Identifying Molecular Markers Associated With Salt And Boron Tolerance In Poplar Trees, Darshanpreet Gill, Nathan Follen, Davis W. Cheng, Kyan Salehi, Gary Banuelos, James P. Prince

STAR Program Research Presentations

Standard irrigation practices promote salt and boron accumulation in soils on the Westside of Central California. The build-up of these inorganic salts leads to the degradation of arable land and reduction of crop production. Different clones of poplar trees have been shown to grow in salt- and boron-contaminated soils, and in doing so, gradually remove significant amounts of these inorganic salts from the soil. In an effort to identify molecular markers linked with salt and boron tolerance in poplars, 31 PCR primer pairs have been designed based on candidate genes identified from the literature, and an additional 31 PCR primer …

From The Laboratory To The Classroom: Characterization Of Microorganisms Residing On Mars-Based Spacecraft, Ryan Hendrickson

STAR Program Research Presentations

Planetary Protection is a discipline that aims to prevent forward biological contamination of other celestial bodies from Earth sources that might interfere with the search and detection of extraterrestrial life on interplanetary missions, such as Mars lander missions. Before any Mars mission with planetary protection bioburden requirements can be launched, the Planetary Protection team at JPL collects samples from the spacecraft and performs the NASA Standard Assay to detect any aerobic, mesophilic microorganisms. My primary objective for the summer was to take 60 isolates from the MER rovers and create long-term glycerol stocks, one working stock, and perform DNA extraction …

Creating A Package In R, Brit Schneiders, Eric Archer

STAR Program Research Presentations

In a time of increasingly efficient technology and data production, scientists are producing data faster than it can be analyzed. Therefore, user accessibility to data analysis is becoming more and more critical. In general, researchers have a set of raw data and want an efficient means to their final analysis. A package serves as that means by creating a set of functions and making them accessible to the user. Often, a user has a small piece of code to run (a single R script, for example), and that script requires the use of certain functions, which are contained in a …

Jellyfish Identification And Quantification In The San Francisco Estuary, Amalia Borson, Lindsay L. Sullivan, Wim Kimmerer

STAR Program Research Presentations

As potential predators and competitors of plankton-eating fish, jellyfish have the potential to negatively impact fish populations. Jellyfish were collected weekly with plankton tows from the RombergTiburonCenterpier in Tiburon, CA. Since some jellyfish were too small to identify, one tow was collected and preserved to record abundances, and a second tow was collected to rear jellyfish until distinguishing characteristics were visible enough for identification. Jellyfish in the preserved tows were then identified, measured, and counted, and their abundance (number m^-3) was calculated. Jellyfish from the second tows were reared in plastic buckets that were lightly bubbled using aquarium …

Preservation Of Biosignature Molecules In Potential Sample Return Container Of The Mars 2020 Mission, Kimberly E. Lykens, Fei Chen Ph.D

STAR Program Research Presentations

Preservation of Biosignature Molecules in Potential Sample Return Container of the Mars 2020 Mission

Kimberly Lykens¹ and Fei Chen²

¹Wittenberg University, Springfield, Ohio 45501 ²Jet Propulsion Laboratory, Pasadena, California, 91109

One requirement for sustainable life on terrestrial planets includes the presence of organic polymers, compounds that are essential for major biological functions such as replication and catalysis. An identified goal of the Mars mission in the year 2020 is to implement a sample-return to identify and validate signs of life on Mars through the discovery of biosignature molecules in Martian core samples. Martian core samples recovered …

Behavioral Variation Between Two Clades Of Leptasterias Spp., Ashley N. Contreras

STAR Program Research Presentations

Leptasterias spp. are six-rayed sea stars found along the rocky intertidal of the northeast Pacific Alaska to Santa Catalina Island, southern California. In central California, three clades of Leptasterias are found in separate or mixed populations, in diverse habitats that range from shallow pools of seagrass and algae to bare rock exposed to crashing waves. Initial field observations of two clades from different locations suggested that behavioral variation may relate to habitat differences among clades. To measure differences in activity, the righting response was timed at both field sites. As a result of behavioral variation observed in the field, more …

Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen

STAR Program Research Presentations

Despite Botrylloides violaceous being a globally invasive species in temperate marine habitats, little is known about it’s natural dispersal behavior. Previous work (Delton et al, 2011) suggests behavioral inferences based on a related and much more commonly studied species may not be appropriate, potentially due to a large size difference between the non-feeding larvae of these two species. Here, we consider factors that may affect dispersal potential. To study how light affects larval release, we measured timing of larval release in the field and compared larval size and time of release. Colonies were collected from floating docks in Richmond, CA …

Estimating Genetic Diversity Of The Invasive Tunicate Didemnum Vexillum In Global Populations, Elizabeth S. Gutierrez, Sarah Cohen

STAR Program Research Presentations

Populations in Point Reyes National Seashore

The presence of the invasive tunicate Didemnum vexillum in the San Francisco Bay was first documented in 1993, and it has since been found in Point Reyes National Seashore. In 2012, 38 samples of D. vexillum were collected from two locations in Point Reyes: Tomales Bay and Drake’s Estero. This summer, DNA from the 38 samples was extracted and analyzed using microsatellite and mitochondrial markers. The goal of this analysis was to determine the level of genetic connectivity between these two populations and their relationship to other global populations in order to answer important …

The Effects Of Microfluidization And Homogenization On The Composition And Structure Of Liposomal Aggregates From Whey Buttermilk And Commercial Buttermilk, Tracey Mai T. Nguyen

Master's Theses

Milk derived ingredients from the production of cheese and butter can be used as vehicles for nutrients. Buttermilk is a nutritious product of milk that comes from the churning of cream into butter. One of the advantages of buttermilk is that it is enriched in milk fat globule components, such as phospholipids and forms emulsions with fat when treated with high shear. The objective of this work was to explore the effects of shear on regular buttermilk and whey buttermilk in terms of liposomal aggregate size and chemical composition. The effects of microfluidization at 2000 psi and homogenization at 2000 …

Design, Fabrication And Test Of An Operationally Responsive Aircraft With Niirs Evaluated Imager, Colin Burt

Master's Theses

Unmanned Aerial Systems (UAS) are a growing asset. Currently UAS are on the cutting edge with resources being spent developing the capabilities mostly for military use. This project is intended to create a system for non-defense customers. Specifically, the Operationally Responsive Aircraft (ORA) will appeal to academic institutions, individual consumers, future customers new to the UAS industry, as well as anybody trying to get airtime for custom sensors.

The system developed in this project utilizes dual aluminum external payload bays attached to a ParkZone Radian aircraft. Each external payload bay can contain approximately 500 $\text{cm}^3$, with a height and width …

Lithic Analysis Of Coyote Canyon Mammoth Site Sediments, Kaitlin M. Hill, George V. Last

STAR Program Research Presentations

The landforms and geologic layers of Southeastern Washington record fascinating, unique geologic events, including repeated catastrophic flooding that occurred during the last Ice Age. These floods left behind many distinctive features, and a variety of fossils. After discovery of mammoth-sized bones in 1999, and confirmation that more bones were still in place in 2008, the Coyote Canyon Mammoth Site near Kennewick, Washington was secured for research, and formal excavation began in 2010.

Previous research suggests that the remains are buried in Ice Age flood deposits, which are overlain by eolian sediments, and those in turn overlain by slope wash. We …

Feeding Ecology Of Delta Smelt During A Seasonal Pulse Of Turbidity, William A. Hilton, Aaron Johnson, Wim Kimmerer

STAR Program Research Presentations

The delta smelt (Hypomesus transpacificus) is a small, pelagic fish endemic to the San Francisco Estuary (SFE) and protected under federal and state endangered species acts. This study examines the diet of adult delta smelt during their spawning migration in the winters of 2010 and 2012. Delta smelt and their zooplankton prey were sampled concurrently during a seasonal pulse of turbidity at sites along their migratory route from the low salinity zone in Suisun Bay to the fresher waters of the Sacramento/San Joaquin Delta. Gut contents were identified to the lowest possible taxon and counted, along with zooplankton …

Detection Of Salmonella Enterica, Escherichia Coli O157 And Listeria Monocytogenes Through Bead Based Magpix® Fluidics, Karamveer Singh, Francisco Fernandez, Sonam Sahni, Alejandro Calderón-Urrea

STAR Program Research Presentations

The research is testing the limits of detection and multiplexing with the use of three pathogens with the machine created from Luminex called Magpix. The pathogens are Salmonella enterica, Listeria monocytogenes, and Escherichia Coli O157. The gene that is being focused on for each pathogen is invA for Salmonella, hlyA for listeria, and hlyA for Escherichia Coli. In this research, we started with prepared probes that were attached to the magnetic microsphere beads. Then we extracted and amplify the DNA. Furthermore, the probes were combined to the amplified DNA and used this in Magpix which gave a corresponding MFI value.

Leatherback Hatchling Fitness, Violet Campbell, Shane Morales, Kelly Stewart

STAR Program Research Presentations

Leatherback hatchling fitness on land is essential for their success in entering the ocean without being predated. Two ways fitness on land may be measured are by how fast hatchlings crawl as well as how fast their righting responses are when they are flipped onto their carapaces. Hatchlings from certain nests have slower righting response times along with slower crawl times, both of which would increase the odds of predation and hinder the success of hatchlings reaching the ocean.

Hatchling crawling speed was determined by timing 12 randomly selected hatchlings from each nest to crawl a distance of two meters. …

Smarter Neat Nets, Ryan Swords Dehaven

Master's Theses

This paper discusses a modification to improve usability and functionality of a ge- netic neural net algorithm called NEAT (NeuroEvolution of Augmenting Topolo- gies). The modification aims to accomplish its goal by automatically changing parameters used by the algorithm with little input from a user. The advan- tage of the modification is to reduce the guesswork needed to setup a successful experiment with NEAT that produces a usable Artificial Intelligence (AI). The modified algorithm is tested against the unmodified NEAT with several different setups and the results are discussed. The algorithm shows strengths in some areas but can increase the …

The Stress Revolution: An Examination Of Acculturative Stress And An Emerging Modern Human Stress Response, Jeffrey Nicklas

Social Sciences

In a world that is so fast pace and always changing, the human biological systems have need to adapt just as swiftly. WIth the dependence on culture that humans all share has led to the building of societies with complex rules, ideals, and accepted roles and ways of life. In these complex societies, great cities and governments have arisen but so too have unnoticed changes to human biology. One of these changes is with environmental stressors and the effects it has on the nervous system. The human body, in these modern times, still responds to perceived stressors with the release …

Verifying R Code And Visualizing Power Grid Data: Signature Quality Metrics And Gui Development, Emmanuel C. Herrera, Brett Amidan

STAR Program Research Presentations

Verifying R code and visualizing Power Grid data:

Signature Quality Metrics and GUI development

Author: Emmanuel Herrera

Mentors: Brett Amidan and Landon Sego

There are many bioforensic signatures produced by analytical instruments that are expensive to produce and maintain accuracy. SQM is an R package which will provide subject matter experts with tools that will help them assess the specific quality of signatures and determine their accuracy, utility and cost by simple function calls. Many published academic papers were surveyed on the kinds of metrics already being implemented. Once understood how some metrics measured accuracy, the SQM package was duplicated …