Digital Commons Network^™

October 1, 2014 Cal Poly Report, Public Affairs Office

Cal Poly Report

No abstract provided.

Fluid-Induced Propulsion Of Rigid Particles In Wormlike Micellar Solutions, David A. Gagnon, Nathan C. Keim, Xiaoning Shen, Paulo E. Arratia

Physics

In the absence of inertia, a reciprocal swimmer achieves no net motion in a viscous Newtonian fluid. Here, using tracking methods and birefringence imaging, we investigate the ability of a reciprocally actuated particle to translate through a complex fluid that possesses a network. A geometrically polar particle, a rod with a bead on one end, is reciprocally rotated using magnetic fields. The particle is immersed in a wormlike micellar (WLM) solution that is known to be susceptible to the formation of shear bands and other localized structures due to shear-induced remodeling of its microstructure. Results show that the nonlinearities present …

Cal Poly To Host Screening Of California's Environmental History Documentary Oct. 23, Public Affairs Office

Press Releases

No abstract provided.

Undulatory Swimming In Shear-Thinning Fluids: Experiments With C. Elegans, David A. Gagnon, Nathan C. Keim, Paulo E. Arratia

Physics

The swimming behaviour of microorganisms can be strongly influenced by the rheology of their fluid environment. In this manuscript, we experimentally investigate the effects of shear-thinning viscosity on the swimming behaviour of an undulatory swimmer, the nematode Caenorhabditis elegans. Tracking methods are used to measure the swimmer’s kinematic data (including propulsion speed) and velocity fields. We find that shear-thinning viscosity modifies the velocity fields produced by the swimming nematode but does not modify the nematode’s speed and beating kinematics. Velocimetry data show significant enhancement in local vorticity and circulation, and an increase in fluid velocity near the nematode’s tail, compared …

Diversity And Soil-Tissue Elemental Relations Of Vascular Plants Of Callahan Mine, Brooksville, Maine, U.S.A., Margaret R. Mansfield, Nathaniel S. Pope, Glen H. Mittelhauser, Nishanta Rajakaruna

Biological Sciences

Metal-contaminated soils provide numerous stressors to plant life, resulting in unique plant communities worldwide. The current study focuses on the vascular plants of Callahan Mine in Brooksville, ME, USA, a Superfund site contaminated with Cu, Zn, Pb, and other pollutants. One hundred and fifty-five taxa belonging to 50 families were identified, with the Asteraceae (21%), Poaceae (11%), and Rosaceae (9%) as the most species-rich families. Ninety-six species encountered at the Mine were native to North America (62%), including 11 taxa (7%) with rarity status in at least one New England state. Fifty-one species were non-native (33%), including nine taxa (6%) …

Estimating Migration Rates Between Populations Of Zostera Marina In The San Francisco Bay, Elizabeth S. Gutierrez

STAR Program Research Presentations

Eelgrass (Zostera marina) is a highly clonal marine angiosperm that can also reproduce sexually through flowering and seed formation. In a previous study, Fst values from six microsatellite loci suggested that a perennial San Francisco Bay subpopulation at Point Molate (Richmond, California) was able to recover from a drastic 2006 die-off through seed recruitment from neighboring eelgrass subpopulations, changing its reproductive strategy from clonal to sexual. Although Fst measures continue to be widely used in population genetics, the assumptions under which they operate are not always appropriate given certain circumstances, such small population sizes and/or asymmetrical migration rates. Our summer …

Development, Characterization, And Optimization Of Process For Minimal Functional Barrier And Channel Width In Nitrocellulose Paper Microfluidic Diagnostic Platforms, Ryan Daniel Silva

Biomedical Engineering

A central goal of diagnostic microfluidics is to reduce the cost of diagnostic medicine by reducing the equipment and reagents needed to perform diagnostic tests. The literature has demonstrated that a wax printer can be used to pattern nitrocellulose paper with hydrophobic barriers to direct a sample in a defined reaction path, eliminating the need for external pumps and controllers. However, manufacturing methods for minimizing sample volume (and thus reagent volume) in a paper-diagnostic chip have not been well defined. In this work, we experimentally determine manufacturing processes for creating functional features of minimal size—effectively reducing the sample size and …

August 27, 2014 Cal Poly Report, Public Affairs Office

Cal Poly Report

No abstract provided.

Is There Progress Toward Sustainability?: Despite The Inherent Human Resistance To Change., Jens Pohl

Collaborative Agent Design (CAD) Research Center

The theme of this paper is to briefly survey the status of current efforts to maintain our natural environment and then discuss prospects for achieving future sustainability. The author finds that while there has been general recognition of the need for environmental sensitivity and conservation of natural resources, progress toward achieving sustainability goals has been slow. A principal reason for this lack of action is found in the reactive nature of the human species. Situated by biological design in our environment we typically respond to environmental changes only after they have occurred. Accordingly, while a number of revolutionary proposals have …

Jellyfish Throwdown: Invasive Versus Native, Brooke Bemowski, Trisha Huynh, Lindsay J. Sullivan, Wim Kimmerer

STAR Program Research Presentations

The San Francisco Bay Estuary is home to many species of marine and wildlife that create a delicate ecological balance. Invasive jellyfish introduced to the bay though cargo shipping are believed to be competing with the endangered delta smelt for the same food source of copepods. Samples of zooplankton were taken from high and low salinity zones in the San Francisco Bay over three years in months June through February to correlate with the peak of the jellyfish lifecycle. The preserved samples are looked through and jellyfish are removed and recorded. The species of jellyfish is determined using features such …

Salinity And Temperature Distribution Of Jellyfish In The San Francisco Estuary, Trisha Huynh, Brooke Bemowski, Lindsay Sullivan, Wim Kimmerer

STAR Program Research Presentations

Jellyfish are generally characterized by their jelly-like bodies and internal lining (two tissue layers). They found both in the phylum Ctenophora and the phylum Cnidaria. Ctenophores differ from cnidarians primarily due to the rows of “combs”, or cilia, which are used for transportation. Additionally, ctenophores possess sticky cells while cindarians possess stinging cells. Jellyfish depend on zooplankton (small floating aquatic animals) as a food source; as a result, they are potential competitors and predators to plankton-eating fish and may negatively impact fish populations.

As recently as 1950, jellyfish have entered the San Francisco Bay from the Mediterranean Sea (probably …

Phylogenetic Diversity Of Microbial Isolates From The Mars Pathfinder, Kyla Bradylong, Adriana Blachowicz, Parag Vaishampayan, James N. Benardini, Wayne Schubert

STAR Program Research Presentations

As spacecraft are sent to different planets, they take with them microscopic pieces of life from Earth. It is the task of the Biotechnology and Planetary Protection Group to keep as much of this life off other planets as possible as well as document any life that may have been sent. During the construction of the Mars Pathfinder, samples were collected from various locations on the spacecraft to test for contamination. These samples were then isolated, grown, documented, preserved and their 16S rRNA genes were sequenced for identification. The 16S rRNA gene sequence is utilized because it is a highly …

The Path To The Sea: Leatherback Hatchling Orientation At Sandy Point National Wildlife Refuge, Christina Macmillan, Kelly Stewart

STAR Program Research Presentations

Once sea turtle hatchlings emerge from their nest, they must find their way to the ocean by using cues such as a bright horizon and the slope of the beach. While moving toward the water, hatchlings often must navigate past predators and through vegetation, sticks, footprints in the sand, and other dangers such as ghost crab holes. Sometimes hatchlings become confused (or disoriented) and turn in circles to find the right route to the water. Sea turtle hatchlings also may become disoriented as a result of human impacts such as town lights or trash. The purpose of our experiment was …

Determining Force Field Parameters Involved With Metal Organic Framework Synthesis, Marcus A. Tubbs, David Cantu, Vanda Glezakou

STAR Program Research Presentations

Metal organic frameworks (MOFs) are synthetic materials made of a cage-like lattice with consistently spaced pores. The size of these pores are the defining characteristic of a MOF, as it determines which gases are allowed to pass through and which can be trapped. Examples of their potential use can be greenhouse gas sequestration or storage. Currently, the synthesis of MOFs is based on trial-and-error, and the successes are not well understood. We are working on building the theoretical framework that describes how a particular MOF, MIL-101, comes together during synthesis. Our initial approach was to simulate the possible reactions with …

Growing Conditions For Algae, Angeles Mora, Tamar Melkonian, Alejandro Calderon-Urrea

STAR Program Research Presentations

With the growing demands of water in California and the increasing cost of fossil fuels to operate water-cleaning equipment, Algae was cultivated in different concentrations of Nitrogen, Phosphorus, and Potassium (NPK) during different trails to identify the best growing conditions for the removal water contaminates. Before testing algae in waste water, the Algae: Dunaliella Primolecta, Chlorella Vulgaris, and Scenedesmus Dimorphus were cultivated in four different media containing distilled water and plant vitamins. The different mediums used include: Orchid Grow More (20-20-20), Miracle Grow (30-10-10), Murashige & Skoog, and Orchid Bloom Boster (11-35-15). As a result of the investigation, the algae, …

Light Pollution Research Through Citizen Science, John Kanemoto

STAR Program Research Presentations

Light pollution (LP) can disrupt and/or degrade the health of all living things, as well as, their environments. The goal of my research at the NOAO was to check the accuracy of the citizen science LP reporting systems entitled: Globe at Night (GaN), Dark Sky Meter (DSM), and Loss of the Night (LoN). On the GaN webpage, the darkness of the night sky (DotNS) is reported by selecting a magnitude chart. Each magnitude chart has a different density/number of stars around a specific constellation. The greater number of stars implies a darker night sky. Within the DSM iPhone application, a …

Xrf Analyses Of Prehanford Orchards, Komal Rana

STAR Program Research Presentations

Subsequent to 1943, the use of Lead Arsenic was banned from the Orchards standing on the Hanford site. This use of Lead Arsenate pesticide was popular among the orchard owners and was dispersed over the site in a myriad of ways. The presence of the traces of lead and arsenic are found today, more than half a century later. Using a portable X-ray florescence analyzer (XRF), the values of lead and arsenic are evaluated while determining the efficiency of the equipment itself. Samples from different decision sites were collected, with lead arsenic values in the low, high and medium range …

Comparison Of The Promega And Kikkoman Atp Devices, Ryan Hendrickson, James Nick Benardini, Kasthuri Venkateswaran

STAR Program Research Presentations

Planetary Protection (PP) is a scientific discipline that aims to protect other celestial bodies from microorganisms found on Earth by preventing forward and backward contamination. Forward contamination is bringing life from Earth to a given celestial body and backward contamination is preventing contamination of eventual sample return missions from Mars back to Earth. PP verifies that NASA Planetary Protection requirements are met in part via the NASA Standard Assay in order to support the launch approval process and to comply with international law. The NASA Standard Assay verifies these bioburden requirements by quantifying any aerobic, heterotrophic, and heat-shock resistant isolates. …

Mother Convict Cichlids Inflict More Bites Upon An Intruder In Hot Temperatures, Jesse L. Heckendorf

STAR Program Research Presentations

Convict cichlids (Archocentrus nigrofasciatus) have developed to be extremely good parents by protecting their brood. Parental care leads to aggressive biting, chasing, and gill flaring to intimidate known predators. In this experiment, we show that environmental factors, such as the changing of temperature in this case, affect a female convict cichlid’s aggression toward caring for her offspring when an intruder is introduced. Females attack more in warmer water.

Salinity Distribution Of Microplankton In The San Francisco Estuary, Carrie Ann Sharitt, Lindsay Sullivan, Wim Kimmerer

STAR Program Research Presentations

Microplankton are a diverse group of planktonic organisms ranging from 0.02 to 0.2 millimeters. Since the group is defined solely by size, it spans numerous taxonomic groups, including both heterotrophs and autotrophs. Microplankton are abundant in all aquatic ecosystems and are important prey for many organisms, including bivalves, crustaceans, and fish. The San Francisco Bay is truly an estuary as saltwater enters the estuary under the Golden Gate Bridge and freshwater flows in from the Sacramento and San Joaquin Rivers. Thus, there is a gradient of salinity from freshwater (0) in the rivers to saltwater by the Golden Gate Bridge …

Aquaponics And Basil Plant Density, Danika L. Macmaster, Brook Murphy, Dudley Burton

STAR Program Research Presentations

Aquaponics is the use of water from a fish tank to provide nutrients to plants in a soil-less environment. The rocks cultivate the bacteria that turns the ammonia into nitrates that the plants need, and the plant bed functions as a filter for the fish tank. In this study, with the guidance of Doctor Dudley Burton, Doctor Brook Murphy, we researched the optimal plant density for basil in an Aquaponics system. We attempted to control or measure all the other variables to consider, as to isolate the variable of distance between plants. The water flow was spread through the plant …

Habitat Preference Of The Introduced Green Crab Carcinus Maenas, Brittany Susan Daum, Andy Chang

STAR Program Research Presentations

The aim of this experiment was to determine if Carcinus Maenas (European Green Crab) hhas a habitat preference in Seadrift Lagoon located in Stinson Beach, California. This experiment was accomplished by placing crab traps on the two types of substrate in Seadrift (sand and algae). The traps were pulled up every 24 hours and the size and sex of crabs was recorded. The data was analyzed to see if there was a difference in habitat preference based upon size, sex, and number of crabs. Using a Poisson Regression model, the size and sex of crabs were not significantly different between …

Avoided Water Cost Of Electricity Generation For Solar Pv And Wind Technologies In Southern California, Matthew Cohen

Master's Theses

The objective of this thesis is to provide a foundation for evaluating the water costs associated with electricity production to calculate the avoided water cost of energy for solar PV and wind technologies relative to coal, natural gas, nuclear, geothermal, concentrated solar thermal, and biomass. Water consumption is estimated for energy production (fuel extraction and preparation) and electricity generation (power plant operation) using the best available information from published articles. The quantity of water consumed for electricity production is monetized for a Southern California case study based on the water rates of Metropolitan Water District of Southern California (MET), which …

Investigating Hemodynamic Responses To Electrical Neurostimulation, Sean Youra

Master's Theses

Since the 1900s, the number of deaths attributable to cardiovascular disease has steadily risen. With the advent of antihypertensive drugs and non-invasive surgical procedures, such as intravascular stenting, these numbers have begun to level off. Despite this trend, the number of patients diagnosed with some form of cardiovascular disease has only increased. By 2030, prevalence of coronary heart disease is expected to increase approximately by 18% in the United States. By 2050, prevalence of peripheral arterial occlusive disease is expected to increase approximately by 98% in the U.S. No single drug or surgical intervention offers a complete solution to these …

A Rubric For Electrochemical Testing Of Metallic Biomaterials, Frederick G. De La Fuente

Master's Theses

Corrosion is a major factor for the failure of metallic medical implants. Testing a metal’s suseptability to corrosion prior to implantation is key to a successful implantation. Electrochemical processes were used in this study to evaluate the characteristics of corrosion of both AISI 316 stainless steel and titanium alloy Ti6Al4V, welded and non-welded. Linear, potentiodynamic, and cyclic polarization curves were produced by the PARC 2273 potentiostat showing the corrosion tendencies of the metals in four unique solutions 3.5% NaCl, 0.35% NaCl, phosphate buffered saline solution (PBS), and Butterfield phosphate buffered solution (BPS). The concentration of chloride ions in solutions affected …

Examining The Impacts Of State Route 101 On Wildlife Using Road Kill Surveys And Remote Cameras, Sara Ann Snyder

Master's Theses

Roads can negatively impact the survival of wildlife populations through additional mortality from road kill and population fragmentation caused by road avoidance behaviors. The 11.9 mile section of State Route 101 between the towns of San Luis Obispo and Atascadero, CA, USA, cross a mountain lion movement corridor and an area important to maintaining ecological connectivity between protected lands in the Los Padres National Forest to the north and south.

I examined the spatial patterns and landscape and roadway factors associated with road kill occurrence for six taxa; large mammals, mesocarnivores, squirrels, rabbits, birds and raptors. Between 1 May 2009 …

Microvascular Topology And Intravascular Endothelial Cell Labeling In The Gracilis Anterior Muscle Of Balb/C Mice, Paul Heckler Ii

Biomedical Engineering

Peripheral arterial occlusive disease (PAOD) affects approximately 200 million individuals globally. The major underlying cause of PAOD is an inflammatory disease known as atherosclerosis, which results from the build-up of low-density lipoproteins (LDL) in the sub-intimal space. This initiates a complex cascade of events that lead to plaque growth. Plaque growth can then expand into the lumen of the vessel and result in occlusion and/or thrombosis. Symptoms of the disease can include claudication, ulcers, and/or gangrene, although many patients are asymptomatic. Similar to other forms of ischemic disease, risk factors for PAOD include hypertension, diabetes, and smoking. Common treatments include …

Sex Ratio Assessment Of Endangered Kemp’S Ridley Sea Turtle Foraging Populations: Validation Of A Testosterone Elisa For Juvenile Sex Determination, William A. Hilton, Camryn D. Allen

STAR Program Research Presentations

Currently all species of sea turtles are listed as threatened or endangered with extinction under the U.S. Endangered Species Act. In order to effectively construct management approaches we need as much information on various sea turtle populations as possible including demography, genetic origin, and critical habitat. One demographic piece of data that is lacking is the sex ratio of turtle populations in foraging habitats, as this information is integral in determining overall population abundance. Because secondary sex characteristics (i.e. males have longer tails) are not evident until turtles start to reach sexual maturity, the sex of juvenile turtles cannot be …

Nerve Fiber Diameter Measurements Using Hematoxylin And Eosin Staining And Brightfield Microscopy To Assess The Novel Method Of Characterizing Peripheral Nerve Fiber Distributions By Group Delay, Jorge Arturo Vazquez

Master's Theses

Peripheral neuropathies are a set of common diseases that affect the peripheral nervous system, causing damage to vital connections between various parts of the body and the brain and spinal cord. Different clinical conditions are known to selectively impact various size nerve fibers, which often makes it difficult to diagnose which peripheral neuropathy a patient might have. The nerve conduction velocity diagnostic test provides clinically useful information in the diagnosis of some peripheral neuropathies. This method is advantageous because it tends to be minimally invasive yet it provides valuable diagnostic information. However, this test does not determine characteristics of peripheral …

Treatment Of Nitrogen Oxides By Chlorella Vulgaris Algae In Photobioreactors, Steven Shihady

Master's Theses

The effectiveness of algae to treat NO₂and NO in simulated flue gas was tested using Chlorella vulgaris in photobioreactors (PBRs) using NO_xconcentrations between 30 ppm to 780 ppm. NO_xdissolved and reacted in water to form NO₃^-and NO₂^-in the PBR growth medium, providing a nitrogen source that the algae readily assimilated for cell synthesis. Three 20-L photobioreactors were inoculated with a pure culture of C. vulgaris prepared in Bristol growth medium and algae were grown in the PBRs at 25°C and pH of 7.0 in a modified Bristol medium …