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Microfluidic Electrical Impedance Spectroscopy, John J. Foley

Master's Theses

The goal of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created in PDMS via soft lithography. The device measured ethanol impedance initially to show that values remain consistent over time. Impedance values of water and 1% wt. saltwater were compared to show that the device is able to detect changes in impedance, with up to a 60% reduction in electrical impedance in saltwater. Cells were …

An Introduction To Deep Learning, Maria Pantoja, Anahid A. Behrouzi, Drazen Fabris

Architectural Engineering

Deep learning (DL) is currently being researched and implemented to solve civil engineering related problems, including autonomous inspection and inventory of civil infrastructure projects. The article introduces DL, specifically a convolutional neural network and the supervised learning process used to train a model that will enable professional structural engineers to automatically detect types of earthquake damage.

Subject-Specific Finite Element Predictions Of Knee Cartilage Pressure And Investigation Of Cartilage Material Models, Michael G. Rumery

Master's Theses

An estimated 27 million Americans suffer from osteoarthritis (OA). Symptomatic OA is often treated with total knee replacement, a procedure which is expected to increase in number by 673% from 2005 to 2030, and costs to perform total knee replacement surgeries exceeded $11 billion in 2005. Subject-specific modeling and finite element (FE) predictions are state-of-the-art computational methods for anatomically accurate predictions of joint tissue loads in surgical-planning and rehabilitation. Knee joint FE models have been used to predict in-vivo joint kinematics, loads, stresses and strains, and joint contact area and pressure. Abnormal cartilage contact pressure is considered a risk factor …

Hydrodynamics In A Shallow Seasonally Low-Inflow Estuary Following Eelgrass Collapse, Ryan K. Walter, Edwin J. Rainville, Jennifer K. O'Leary

Physics

Hydrodynamics play a critical role in mediating biological and ecological processes and can have major impacts on the distribution of habitat-forming species. Low-inflow estuaries are widespread in arid regions and during the dry season in Mediterranean climates. There is a growing need to evaluate dynamics and exchange processes in these systems and the resultant ecological linkages. We investigate the role that hydrodynamics play in shaping environmental gradients in a short and seasonally low-inflow estuary located along the central California coast. Since 2007, eelgrass meadows in Morro Bay have declined by more than 90%, representing the collapse of the major …

Inverse Dynamic Analysis Of Acl Reconstructed Knee Joint Biomechanics During Gait And Cycling Using Opensim, Megan V. Pottinger

Master's Theses

ACL (anterior cruciate ligament) injuries of the knee joint alter biomechanics and may cause abnormal loading conditions that place patients at a higher risk of developing osteoarthritis (OA). There are multiple types of ACL reconstruction (ACLR), but all types aim to restore anterior tibial translation and internal tibial rotation following surgery. Analyzing knee joint contact loads provide insight into the loading conditions following ACLR that may contribute to the long-term development of OA. Ten ACLR subjects, who underwent the same reconstruction, performed gait and cycling experiments while kinematic and kinetic data were collected. Inverse dynamic analyses were performed on processed …

Detecting A Probiotic Product Within The Gut Of Broiler Chickens, Anneka Pisula

Master's Theses

As of January 2017, the U.S. poultry industry banned the use of antibiotics and now relies on alternatives such as probiotics to help protect animal health. Although probiotic use is not a new concept in the poultry industry, identifying the best combination of bacterial strains to generate an effective probiotic formula requires further investigation. This study aimed to detect a probiotic product of four bacterial strains (Pedioccoccus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, and Bacillus subtilis) in a feeding trial with broiler chickens. Birds given the probiotic were predicted to show an improved growth performance with …

Quantifying The Environmental Performance Of A Stream Habitat Improvement Project, Cody Morse

Master's Theses

River restoration projects are being installed worldwide to rehabilitate degraded river habitat. Many of these projects focus on stream habitat improvement (SHI), and an estimated 60%of the 37,000 projects listed in the National River Restoration Science Synthesis Program focus on SHI for salmon and trout species. These projects frequently lack a sufficient monitoring program or account for the environmental costs associated with SHI. The present study used life cycle assessment (LCA) techniques and topographic effectiveness monitoring to quantify environmental costs on the basis of geomorphic change. This methodology was a novel approach to assessing the cost-benefit relationship of SHI. To …

Assessment Of Granulovirus, Spinosad, And Mating Disruption For Controlling Cydia Pomonella L. [Lepidoptera: Tortricidae] In Organic Coastal California Apple Orchards, Raven Lukehart

Master's Theses

Codling moth, Cydia pomonella[Lepidoptera: Tortricidae], is a major entomological pest of apples, pears, and walnuts cross the world (Pajac et al. 2016). Female codling moths lay eggs on the apple exocarp and larvae burrow within the fruit causing economic losses to fruit growers.Organic apple orchards in San Luis Obispo, CA currently have three codling moth, Cydia pomonella,control options commercially available including granulovirus (CpVG), spinosad, and mating disruption. In field tests on apple (Malus), we compare percent fruit injury between treatments of granulovirus (2.43 oz/ha Cyd-X® organically approved, Certis USA, Columbia MD), spinosad (4.05 oz/ha Entrust® Naturalyte® …

Evaluation Of Endothelial Cell Responses To Elevated Glucose, Gabriella Sugerman

Master's Theses

Developing a tissue-engineered Blood Vessel Mimic (BVM) to represent diabetic macrovascular disease could expedite design of new vascular devices specifically tailored to diabetic patients. In contribution toward this model, this thesis assessed Human Umbilical Vein Endothelial Cell (HUVEC) responses to high glucose conditions. Interleukin 6 (IL-6) and Cluster of Differentiation 36 (CD36) were selected to signify oxidative stress activity, a hallmark of diabetic macrovascular disease. Next, activity of potential reference genes B2M, HPRT1, and ACTB was assessed. All genes were found to exceed acceptable variability, so the E-ΔC T method of data analysis was selected. Next, cellular responses to high …

Crossing Corridors: Wildlife Use Of Jumpouts And Undercrossings Along A Highway With Wildlife Exclusion Fencing, Alex J. Jensen

Master's Theses

Roads pose two central problems for wildlife: wildlife-vehicle collisions (WVCs) and habitat fragmentation. Wildlife exclusion fencing can reduce WVCs but can exacerbate fragmentation. In Chapter 1, I summarize the relevant studies addressing these two problems, with a focus on large mammals in North America. Chapters 2 and 3 summarize field assessments of technologies to reduce WVCs and maintain connectivity, specifically jumpout ramps and underpasses, along Highway 101 near San Luis Obispo, CA. In a fenced highway, some animals inevitably breach the fence and become trapped, which increases the risk of a wildlife-vehicle collision. Earthen escape ramps, or “jumpouts”, can allow …

More Rapid Increase In Bmi From Age 5–15 Is Associated With Elevated Weight Status At Age 24 Among Non-Hispanic White Females, Katherine N. Balantekin, Emily E. Hohman, Elizabeth L. Adams, Michele E. Marini, Alison K. Ventura, Leann L. Birch, Jennifer S. Savage

Kinesiology and Public Health

Background: A rapidly increasing BMI trajectory throughout childhood is associated with negative health outcomes in adulthood such as obesity, cardiovascular disease, and diabetes. The purpose of the current study was to assess whether BMI trajectories from age 5–15 predicted changes in weight and BMI from adolescence to adulthood, and dieting-related behaviors in young adulthood.

Methods: Non-Hispanic White female participants from Early Dieting in Girls (n=182), a longitudinal cohort study, were followed from age 5 to 15 and completed a follow-up survey at age 24. Participants were classified by age 5–15 BMI trajectory groups: UPC, accelerated weight gain from age …

A Zoopolean Look At Animal Research Ethics, Andrew T. Fenton

Between the Species

I will discuss how animal laboratory research can be ethically analyzed using Donaldson and Kymlicka’s political theory of animal rights. To accomplish this, I will not presuppose their strong animal rights framework. Donaldson and Kymlicka’s approach revolves around some basic human-animal relationships, reflecting the relational turn in applied ethics writ large. However, they do not discuss laboratory animal research in any detail, and so an extension to that domain of animal use is in order. Donaldson and Kymlicka’s emphasis on human-animal relationships is useful for reminding ourselves that in laboratories various staff or personnel can develop bonds with captive animals …

The Effect Of Destoning And Enzymatic Pretreatments On The Biofuel Production From Olive Cake, Patrick Tai

Master's Theses

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed an …

Beyond Human Intelligence: Redefining Intelligence, Jens Pohl

Architecture

The digital revolution driven by global connectivity, computing as a utility, artificial intelligence, and information sharing on an unprecedented scale is forcing the redefinition of intelligence in a broader context that extends beyond human intelligence. This paper addresses the principal characteristics of biological intelligence, as it exists on planet Earth and the form it may take in an extraterrestrial context. Human intelligence, as the most evolved form of biological intelligence on Earth, is treated as the aggregation of multiple intelligences that cannot be encapsulated in a single metric such as an Intelligence Quotient (IQ). The concept of a postbiological civilization …

Adjusting To The New Abet Criteria 3 And 5: It’S Really Not Very Hard, Allen C. Estes, Peter Laursen, Pamalee A. Brady

Architectural Engineering

ABET has revised the criteria 3 (Student Outcomes) and 5 (Curriculum) of the general criteria. These changes have made it through the rigorous approval process and will be effective for the 2019-20 academic year. This paper analyzes the differences between the existing criteria and the proposed changes. Through a specific detailed example, the paper illustrates how an existing assessment system can seamlessly be adjusted for the new criteria. Most of the embedded indicators and direct measures of attainment can still be used. The results simply need to be organized differently.

Multidisciplinary Research Efforts In Post-Earthquake Civil Infrastructure Reconnaissance, Jack Bergquist, Anahid A. Behrouzi

Architectural Engineering

To address existing challenges with filtering and classification of post-earthquake structural damage images, the authors are engaged in a multidisciplinary project to develop and train a machine-learning algorithm that identifies relevant photographs and assigns damage tags to those images. The research team is predominantly comprised of undergraduate students and is led by a structural engineering and a computer science faculty. While machine-learning algorithms have been successfully used for image tagging in a variety of fields (health care, manufacturing, etc.), the extension of this approach for earthquake reconnaissance is only just beginning. As such, the creation and development of this tool …

Academic Senate - Agenda, 6/5/2018, Academic Senate

Academic Senate Agendas

No abstract provided.

Comparative Analysis Of Physiological Measurements And Environmental Metrics On Predicting Heat Stress Related Events, Mckenzie Barlow

Baker/Koob Endowments Awarded Projects

Exposure to high heat and humidity can lead to serious health risks, including heat stroke and other heat-stress related events. Wet Bulb Globe Temperature has historically been used to predict heat stress events, but individualized factors are not included in the measurement. It has been shown that there is a relationship between cardiovascular measurements and heat stress, which could be used to measure on an individual level. Research has been done to find relationships between cardiovascular metrics in a workplace environment, however the study did not include the use of a controlled environment as a baseline. Our study will provide …

Preparation And Characterization Of Eva/Cort Microparticles For Rattlesnake Endocrinology Studies, Alexander C. Pauer

Materials Engineering

No abstract provided.

Column Tests Of Nitrate Breakthrough Behavior In Subsurface Sediments To Understand Transport In The Root-Zone, Rebecca Sally Haworth, Emma Rose Goodwin

Natural Resources Management and Environmental Sciences

Nitrate has become an increasingly ubiquitous pollutant in surface and groundwater, posing a threat to hu- man health and ecosystems. Nitrogen is a necessary nutrient for plant growth and is limiting in many soils. As a result, farmers often add nitrogen to soil in a usable form such as nitrate, nitrite, or ammonia through the addition of fertilizer.

Genetic Algorithm Amplifier Biasing System (Gaabs): Genetic Algorithm For Biasing On Differential Analog Amplifiers, Sean Whalen

Computer Engineering

Genetic Algorithm Amplifier Biasing System (GAABS) - Senior Project Analysis

Summary of Functional Requirements

This project integrates LTSpice with a python script that runs a genetic algorithm to bias a differential amplifier. The system biases the amplifier with 2 different voltages, the base voltage for the PNP BJTs of the active loads and a voltage controlling the current of the current sink. The project runs via a python script, gets data from LTSpice’s command line call, and iteratively runs until the system is biased to achieve the greatest gain on an arbitrary input voltage.

Primary Constraints

Some of the main …

Analysis Of Potential Vermicompost Market In California, Jason Alan Thomas

Master's Theses

Vermicompost transforms agricultural waste into a high quality soil amendment, though market acceptance remains in its infancy. This study examines how growers’ willingness to pay for vermicompost is affected by grower’s crop, region, income per acre, knowledge level of vermicompost and compost, previous use of compost, and the willingness to pay for compost. The survey results pulled together 223 responses from California growers. It was discovered growers’ had less knowledge of vermicompost than compost but were willing to pay more for vermicompost. There was statistical difference amongst the responses. It was shown the market value per ton of vermicompost lies …

Connected Alerts Across Manufacturing Facilities, Jordan J. Moy

Industrial and Manufacturing Engineering

The lack of communication during a large project can result in costly delays when striving to achieve previously defined benchmarks. Often times, each part of a project will require verification from an employee of a specified area of expertise before being allowed to advance to the next stage. Finding these individuals can be extremely hard due to the large, spread out campus of manufacturing facilities as well as the numerous projects the company is concurrently working on. These floor support requests can sometimes take from an hour to even a whole work day, causing major delays in the production schedule. …

Process Improvement At "The Manse On Marsh", Kevin C. Manko

Industrial and Manufacturing Engineering

The Manse on Marsh is an independent and assisted living facility that prides itself on providing life-enriching care for the elderly. Considering that the staff is essential to supply this care and turnover rates are a constant factor, the company would like to reduce the time it takes to hire new employees by at least one week. To facilitate this goal, process flow maps are utilized to assess the current state of the hiring process and metrics are applied to support future state design decisions. After performing a value added analysis and identifying areas of waste, a total of 20 …

High Bay Capacity Utilization Tool, Dante C. Mazzanti, Skyler L. Schilke, Conner P. Lawston

Industrial and Manufacturing Engineering

Northrop Grumman’s Space Park facility has many inefficiencies in their high bay scheduling processes. Additionally, it is very hard for project managers to figure out the capabilities of each high bay in order to schedule their specific project. The purpose of this project is to develop a tool that can be used across the facility to easily create projects, assign projects, view room capabilities, and view a master schedule of the rooms. After researching commercially available tools, our approach to designing this system was to use tools that are already utilized at the facility (Microsoft Suite and Tableau) and engineer …

Reducing Overall Overtime Hours Accumulated By San Luis Sourdough, Marie Scholl, Emily Vallejos, Randall Yonce

Industrial and Manufacturing Engineering

The packaging department of San Luis Sourdough Company was redesigned to increase bread throughput and decrease overtime hours issued to employees. Time studies were performed to get an accurate representation of the average throughput of bread with the current packaging conveyor system. After collecting data and surveying the employees and management, we came up with three design solutions to improve their current packaging system. The first design recommendation has a low-investment and is quick to implement as it pertains to changing the worker’s schedule to alleviate the employees from working long hour shifts while increasing the bread throughput. The second …

Vial Of Life Redesign, Andrew Scott Heim, Marisonn Manalo

Industrial and Manufacturing Engineering

Cambria Anonymous Neighbors (CAN), a nonprofit organization assisting the residences of Cambria, hopes to standardize first responder procedures and make medical information easily accessible in case of a medical emergency. Currently the container costs $3.78. CAN would like to provide 2000 containers at a lower cost. After researching materials, acrylic was found to be the best option for the container material due to its low cost and durability. A laser cutter was used to cut the acrylic pieces, and then a set of step by step instructions were created to assemble the product. To reduce the cost of acrylic, the …

Soil Science In Precision Agriculture Lesson Plan, Carson Ellis Cronkright

Agricultural Education and Communication

Due to the increasing demands for the American agricultural industry to provide exceptional products at an increasing rate of production, as well as implement the practice of sustainable farming, it is imperative that more modern concepts be taught at a younger age (Ray, 2013). In order to inform students of advancements and the future of agriculture in the United States, curriculum and lesson plans should reflect this movement in industry in order to provide relevant information and maintain the highest quality of education possible. This project will provide a constructed lesson plan as one example of adopting more progressive methods …

Implementing Restorative Justice Programs In The Cal Poly Community, Bryce R. Fauble Iii

Liberal Arts and Engineering Studies

This paper is the result of a year-long senior project for the Liberal Arts and Engineering program at California Polytechnic State University. This paper attempts to educate the reader on what Restorative Justice is, why it faces challenges in the United States, and how it has been implemented, both in the United States and outside of it. In addition, this paper describes my own experience with implementing Restorative Justice Programs with both the city of San Luis Obispo and California Polytechnic State University. This experience includes the challenges that I faced along the way, and how these challenges are indicative …

Facilities Redesign Of Stockroom At Elcon Precision, David M. Brawner, Tyler O. Mastromattei, Jay Y. Matsumoto

Industrial and Manufacturing Engineering

Elcon Precision is a company based out of San Jose, California specializing in the photochemical processing of materials and brazing of metals and ceramics for a variety of different industries. Three students from the Industrial Engineering Department at California Polytechnic State University, San Luis Obispo were contacted by Elcon Precision to help redesign their stockroom and inventory areas in their San Jose location in order to allow for the future growth of the company. Elcon asked the Cal Poly project team to help increase efficiencies throughout the stockroom by creating an alternative layout that best suited the company's needs. This …