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Biomechanics Of Crop Plants, Douglas Cook Aug 2018

Biomechanics Of Crop Plants, Douglas Cook

BYU Research Development Office Research Networking Conference

Modeling biological systems, stochastic modeling, sensitivity analyses, uncertainty quantification, statistical shape modeling, biomechanics in general, applying engineering techniques to new problems, graphic design, undergraduate training methods


An Ecological And Distributional Analysis Of Great Basin Bristlecone Pine (Pinus Longaeva), Gregory Watson Taylor Aug 2018

An Ecological And Distributional Analysis Of Great Basin Bristlecone Pine (Pinus Longaeva), Gregory Watson Taylor

Theses and Dissertations

Understanding the impacts of climate change is critical for improving the conservation and management of ecosystems worldwide. Ecosystems vary along a precipitation and temperature gradient, ranging from tropical jungles to arid deserts. The Great Basin is a semi-arid eco-region that is found within the western United States. Plant communities within the Great Basin range from sagebrush valleys to sub-alpine conifer forests found at high elevation areas. It is predicted that the Great Basin will experience prolonged periods of drought, more intense fires, and greater variability in average annual and monthly precipitation, all in response to changes in climate patterns. At …


Electrochemical Glucose Sensors Enhanced By Methyl Viologen And Vertically Aligned Carbon Nanotube Channels, Benjamin J. Brownlee, Meisam Bahari, John H. Harb, Jonathan C. Claussen, Brian D. Iverson Aug 2018

Electrochemical Glucose Sensors Enhanced By Methyl Viologen And Vertically Aligned Carbon Nanotube Channels, Benjamin J. Brownlee, Meisam Bahari, John H. Harb, Jonathan C. Claussen, Brian D. Iverson

Faculty Publications

Free-standing, vertically aligned carbon nanotubes (VACNTs) were patterned into 16 μm diameter microchannel arrays for flow-through electrochemical glucose sensing. Non-enzymatic sensing of glucose was achieved by the chemical reaction of glucose with methyl viologen (MV) at an elevated temperature and pH (0.1 M NaOH), followed by the electrochemical reaction of reduced-MV with the VACNT surface. The MV sensor required no functionalization (including no metal) and was able to produce on average 3.4 electrons per glucose molecule. The current density of the MV sensor was linear with both flow rate and glucose concentration. Challenges with interference chemicals were mitigated by operating …


Developable Compliant-Aided Rolling-Contact Mechanisms, Todd G. Nelson, Just L. Herder Aug 2018

Developable Compliant-Aided Rolling-Contact Mechanisms, Todd G. Nelson, Just L. Herder

Student Works

Rolling-contact mechanisms can provide low-friction motion with unique kinematic paths. We show that developable surfaces can be used as a design tool for rolling-contact mechanisms joined with compliant bands. These mechanisms can exhibit 3D motion paths, couple rotational and translational motions into a single degree of freedom, and can be designed to exhibit various tailored kinetic responses. We set forth developable surface parametrizations well suited to the creation of rolling contacts. We highlight how the geodesic and principal curvatures of the non-ruling principal curves of a developable surface are meaningful design quantities for rolling contacts. We provide kinematic and kinetic …


Video Prompting Delivered Via Augmented Reality To Teach Transition-Related Math Skills To Adults With Intellectual Disabilities, Giulia Cacciatore Aug 2018

Video Prompting Delivered Via Augmented Reality To Teach Transition-Related Math Skills To Adults With Intellectual Disabilities, Giulia Cacciatore

Theses and Dissertations

The purpose of this study was to determine the effectiveness of a video-based instruction packet as a method of teaching math-based vocational skills delivered through augmented reality to three adults with intellectual disabilities. The dependent variable was the percentage of steps performed correctly to solve each selected type of math problem. The independent variable was the video-based math intervention delivered via augmented reality, which modeled the individual steps for solving three different multi-step math problems: (1) adjusting a recipe to serve a different number of people, (2) calculation of salary, (3) calculation of unit prices. Visual and statistical analysis demonstrated …


Organic Synthesis, Molecule Design, Chemical Manufacturing, David J. Michaelis Aug 2018

Organic Synthesis, Molecule Design, Chemical Manufacturing, David J. Michaelis

BYU Research Development Office Research Networking Conference

Areas of Interest: Design and synthesis of bioactive molecules; Catalysis with transition metal, nanoparticle, and enzyme catalysts; Molecular design in drug discovery, materials chemistry, and air-free chemistry


Soft Nanotechnology, Wally Paxton Aug 2018

Soft Nanotechnology, Wally Paxton

BYU Research Development Office Research Networking Conference

Areas of Interest: Designing stimuli-responsive amphiphilic block copolymers, tuning the chemical and electronic properties of interfaces, and integrating functional biomolecules in synthetic matrices


Exploration Of Antimicrobial Activity In Natural Peptides And High-Throughput Discovery Of Synthetic Peptides, Emma Kay Dallon Aug 2018

Exploration Of Antimicrobial Activity In Natural Peptides And High-Throughput Discovery Of Synthetic Peptides, Emma Kay Dallon

Theses and Dissertations

Despite many medical advances, antibiotic resistant bacteria increasingly plague the modern world, necessitating discovery of new antibiotics. One area of nature that can provide inspiration for antibiotics is antimicrobial peptides. Many of these peptides exist in nature, with some classes that have not been studied or characterized well. One such class is the defensin-like peptides generated by the plant Medicago truncatula as part of their symbiotic relationship with Sinorhizobium meliloti. Nodule-specific Cysteine Rich (NCR) peptides are defined by the presence of multiple cysteines, and regulate the growth of S. meliloti within plant cells. While some of these NCR peptides have …


Species Delimitation Predictions Using Mitochondrial And Nuclear Dna Sequences From The Heteromys Pictus-Spectabilis Species Complex, Joanna Rosa Bateman Aug 2018

Species Delimitation Predictions Using Mitochondrial And Nuclear Dna Sequences From The Heteromys Pictus-Spectabilis Species Complex, Joanna Rosa Bateman

Theses and Dissertations

Heteromys pictus-spectabilis is a species complex within the subfamily Heteromyinae (Family: Heteromyidae) that is distributed along the western and southern Mexican coast and surrounding environments. Currently, the species complex is accepted as being 2 separate species (H. pictus and H. spectabilis), but this also renders H. pictus paraphyletic. Therefore, the species complex requires re-evaluation in order to resolve the paraphyly. Mitochondrial DNA sequences from a previously existing ~720 specimen database compiled by Victoria Vance were used in conjunction with new nuclear DNA sequences sequenced for the purpose of this study to generate multiple phylogenetic trees via the software programs RAxML, …


The Responses Of Ants And Other Invertebrates To Fire And Rodent Activity In North American Deserts, Joshua David Day Aug 2018

The Responses Of Ants And Other Invertebrates To Fire And Rodent Activity In North American Deserts, Joshua David Day

Theses and Dissertations

Human activities are increasing the size, frequency and severity of disturbance across earth’s ecosystems including deserts. Exotic annual grasses have altered fire regimes by increasing the size, frequency, and severity of fires in these systems. Invertebrates make up a large proportion of ecosystem diversity, provide a wide range of ecosystem functions, and are good indicators of ecosystem function and resilience. Ants are particularly good indicators of ecosystem stability. The ability of rodents to modify plant community structure post-fire, could result in rodent communities having important indirect effects on invertebrate communities. In chapter 1 we report changes in ant forager abundance …


Fluorescent Labeling Of Antibiotic Resistant Bacteria Model Dna, Janice Darko Aug 2018

Fluorescent Labeling Of Antibiotic Resistant Bacteria Model Dna, Janice Darko

Theses and Dissertations

Global threats to treatment of bacterial infections due to antibiotic resistance (AR) have been on the rise in recent years. Current diagnostic tests identify bacteria by using blood culture, which takes more than 24 hours. This study focuses on the fluorescent labeling of DNA derived from bacterial AR genes (KPC & VIM) and other model DNAs using oligreen dye (OG) and molecular beacons (MB). A NanoDrop 3300 fluorospectrometer was used to take fluorescence measurements. Linear dynamic range and labeling efficiency were dependent on the following optimized conditions: dilution factor of OG (200 fold), buffer (20 mM Tris HCl, pH 8), …


The Effects Of Ice And Tens Combination Treatment On Knee And Hip Joint Neuromechanics In Individuals With Experimentally Induced Knee Pain During Running, Sunku Kwon Aug 2018

The Effects Of Ice And Tens Combination Treatment On Knee And Hip Joint Neuromechanics In Individuals With Experimentally Induced Knee Pain During Running, Sunku Kwon

Theses and Dissertations

Context: Knee injury is a common problem for runners. Knee pain is a common symptom in knee injury and is associated with alterations in knee and hip muscle activation and hip joint angles. Relieving pain through intervention may help to restore neuromuscular function. Objective: To examine the effects of ice and transcutaneous electrical nerve stimulation (TENS) combination treatment on perceived knee pain, hip frontal plane angle, and muscle activation during running in individuals with experimental knee pain (EKP). Design: Crossover. Setting: Laboratory. Subjects: 19 participants (11 males and 8 females, 23.2 ± 1.9 y, 176 …


Composition Of The Community Of Small Mammals In The Great Basin Desert, Samantha Elizabeth Phillips Aug 2018

Composition Of The Community Of Small Mammals In The Great Basin Desert, Samantha Elizabeth Phillips

Theses and Dissertations

Small mammals are a keystone guild in arid ecosystems; often exhibiting top-down control of the diversity and structure of plant communities. However, changing climate, shifting fire regimes, and the invasion of exotic plants are modifying the structure of arid systems. Environmental changes in these arid systems are likely altering small mammal communities, and therefore, their ecological role. We examined two aspects of the community composition of small mammals in the Great Basin: changes in community composition since large scale sampling of the region began in 1930, and the current population of a sensitive species of small mammal, the dark kangaroo …


Neuroimmune-Mediated Alcohol Effects On Ventral Tegmental Area Microglia And Infiltrating Leukocytes, Travis Jonathan Clarke Aug 2018

Neuroimmune-Mediated Alcohol Effects On Ventral Tegmental Area Microglia And Infiltrating Leukocytes, Travis Jonathan Clarke

Theses and Dissertations

Microglia are the primary immune cell in the central nervous system and are known as the “resident” macrophages in the central nervous system (CNS). While microglia are classically known as the immune cells of the CNS, their role has more recently been shown to extend far beyond immunity. The effects of ethanol on the brain are closely linked to neuroimmune responses mediated by microglia that are present in the healthy brain from the time of development. Though microglia have been classified as the “resident” immune cells of the CNS, new research suggests that other immune cells may be implicated in …


A Graph Theoretical Analysis Of Functional Brain Networks Related To Memory And Healthy Aging, Ty Alvin Bodily Aug 2018

A Graph Theoretical Analysis Of Functional Brain Networks Related To Memory And Healthy Aging, Ty Alvin Bodily

Theses and Dissertations

The cognitive decline associated with healthy aging begins in early adulthood and is important to understand as a precursor of and relative to mild cognitive impairment and Alzheimer disease. Anatomical atrophy, functional compensation, and network reorganization have been observed in populations of older adults. In the current study, we examine functional network correlates of memory performance on the Wechsler Memory Scale IV and the Mnemonic Discrimination Task (MST). We report a lack of association between global graph theory metrics and age or memory performance. In addition, we observed a positive association between lure discrimination scores from the MST and right …


Modeling Coastal Vulnerability For Insight Into Mangrove And Coral Reef Conservation Efforts In Cuba, Maria Gomez Aug 2018

Modeling Coastal Vulnerability For Insight Into Mangrove And Coral Reef Conservation Efforts In Cuba, Maria Gomez

Theses and Dissertations

Cuba’s expansive coral reefs and mangrove habitats provide a variety of ecosystem services to coastal communities including nursery grounds for fisheries, shoreline stability, and storm and flood protection. While Cuba’s coastal habitats are some of the most preserved in the Caribbean, they are under increasing threat of degradation from the impacts of climate change, increased tourism, and coastal development. With the goal of sustainable development, Cubans need to assess the storm and flood protection benefits these coastal habitats provide, and integrate this information into future expansion and management plans within the National Protected Areas System (SNAP). Using the open source …


Methods For Detection Of And Therapy For Carbapenem-Resistant Enterobacteriaceae, Olivia Tateoka Brown Aug 2018

Methods For Detection Of And Therapy For Carbapenem-Resistant Enterobacteriaceae, Olivia Tateoka Brown

Theses and Dissertations

As antibiotic resistant bacterial strains are becoming more prevalent in healthcare settings, it is necessary to find alternative methods of detecting and treating these infections. One of the antibiotic resistant strains of interest is the carbapenem-resistant Enterobacteriaceae (CRE). CREs have the ability to evade some of the most potent antibiotics currently in use and employ carbapenemases to negate the effect of antibiotics. The three most common carbapenemase genes, found in carbapenem-resistant Enterobacteriaceae along with a gene found only in Escherichia coli were chosen to create a qPCR assay for rapid detection of resistant infections. The carbapenemase genes are KPC, VIM …


A Dual Examination Of Learning Through Pedagogical Training And Alzheimer's Disease Pathology, Donielle Breanna Hutchinson Aug 2018

A Dual Examination Of Learning Through Pedagogical Training And Alzheimer's Disease Pathology, Donielle Breanna Hutchinson

Theses and Dissertations

Active learning strategies are important for facilitating deep learning that may be carried throughout life, but which is still finding its way into the college setting. Educators are not often trained in effective learning practices, which reduces the cognitive and proficiency gains of their students. By providing such guidance in the formative years of a teacher’s training, we hypothesize that the learning environment will be greatly enriched and enhanced. On the opposite end of the spectrum of life and cognition, the plague of dementia also warrants examination. Alzheimer’s disease (AD), an incurable neurodegenerative disorder progressing from the medial temporal lobe, …


Electrochemical Glucose Sensors Enhanced By Methyl Viologen And Vertically Aligned Carbon Nanotube Channels, Benjamin J. Brownlee, Meisam Bahari, John N. Harb, Jonathan C. Claussen, Brian D. Iverson Aug 2018

Electrochemical Glucose Sensors Enhanced By Methyl Viologen And Vertically Aligned Carbon Nanotube Channels, Benjamin J. Brownlee, Meisam Bahari, John N. Harb, Jonathan C. Claussen, Brian D. Iverson

Faculty Publications

Freestanding, vertically aligned carbon nanotubes (VACNTs) were patterned into 16 μm diameter microchannel arrays for flow-through electrochemical glucose sensing. Non-enzymatic sensing of glucose was achieved by the chemical reaction of glucose with methyl viologen (MV) at an elevated temperature and pH (0.1 M NaOH), followed by the electrochemical reaction of reduced-MV with the VACNT surface. The MV sensor required no functionalization (including no metal) and was able to produce on average 3.4 electrons per glucose molecule. The current density of the MV sensor was linear with both flow rate and glucose concentration. Challenges with interference chemicals were mitigated by operating …


“It Is A Privilege To Pee”: The Rise And Demise Of The Pay Toilet In America, Katie Richards Jul 2018

“It Is A Privilege To Pee”: The Rise And Demise Of The Pay Toilet In America, Katie Richards

The Thetean: A Student Journal for Scholarly Historical Writing

No abstract provided.


Roost Characteristics And Clustering Behavior Of Western Red Bats (Lasiurus Blossevillii) In Southwestern New Mexico, Brett R. Andersen, Keith Geluso Jul 2018

Roost Characteristics And Clustering Behavior Of Western Red Bats (Lasiurus Blossevillii) In Southwestern New Mexico, Brett R. Andersen, Keith Geluso

Western North American Naturalist

The western red bat (Lasiurus blossevillii) is a foliage-roosting species of riparian habitats in arid regions of the southwestern United States. Only limited published anecdotal observations exist for roost sites used by this species. Western red bats were split taxonomically from the eastern red bat (Lasiurus borealis) in 1988, but summaries of roosting behaviors for western red bats still appear to stem from former associations with the commonly studied eastern red bat. Our study represents the first comprehensive research on the roosting ecology of western red bats. We radio-tracked 14 adult bats to 19 different day …


New State Record And Potential Distribution Of The Snake Sibon Nebulatus From Mexico, Luis Badillo-Saldaña, Jose Daniel Lara-Tufiño, Aurelio Ramírez-Bautista, Jonathan A. Campbell Jul 2018

New State Record And Potential Distribution Of The Snake Sibon Nebulatus From Mexico, Luis Badillo-Saldaña, Jose Daniel Lara-Tufiño, Aurelio Ramírez-Bautista, Jonathan A. Campbell

Western North American Naturalist

We report the northernmost Atlantic Coast record of the Cloudy Snail Sucker (Sibon nebulatus) from the state of Hidalgo, at a straight-line distance of about 185-km from the nearest historical locality in Sierra Norte de Puebla, Mexico. We assess the potential distribution of this species and compare morphological characteristics of specimens from Mexico and Central America. No significant morphometric or meristic differences were found. A species distribution model suggests that S. nebulatus is widely distributed on both coasts of Mexico, with a high probability of occurrence at a wider range of latitudes than current records indicate.


Reportamos el …


Bat Hibernacula In Caves Of Southern Idaho: Implications For Monitoring And Management, Jericho C. Whiting, Bill Doering, Gary Wright, Devin K. Englestead, Justin A. Frye, Todd Stefanic Jul 2018

Bat Hibernacula In Caves Of Southern Idaho: Implications For Monitoring And Management, Jericho C. Whiting, Bill Doering, Gary Wright, Devin K. Englestead, Justin A. Frye, Todd Stefanic

Western North American Naturalist

Bat populations are being impacted by many threats, including white-nose syndrome, wind energy development, and hibernaculum disturbance and modification. Understanding the use of caves as hibernacula by bats in the western United States is necessary for the conservation and management of these mammals and their habitat, as well as for monitoring the arrival of white-nose syndrome. We identified biologically important hibernacula from 304 winter surveys (1 November–31 March) in 64 caves from 1984 to 2016 in southern Idaho, USA. During surveys researchers counted 37,693 bats representing 6 species. Townsend’s big-eared bats (Corynorhinus townsendii townsendii) comprised 96.1% (36,237 individuals …


First Description Of A Bald Eagle (Haliaeetus Leucocephalus) Actively Depredating An Adult Sandhill Crane (Antigone Canadensis), Andrew J. Caven, Joshua D. Wiese, William R. Wallauer, Kristin J. Mosher Jul 2018

First Description Of A Bald Eagle (Haliaeetus Leucocephalus) Actively Depredating An Adult Sandhill Crane (Antigone Canadensis), Andrew J. Caven, Joshua D. Wiese, William R. Wallauer, Kristin J. Mosher

Western North American Naturalist

From late February to early April the Central Platte River Valley in Nebraska is home to the largest gathering of Sandhill Cranes (Antigone canadensis) in the world and increasingly serves as a foraging ground for migrating Bald Eagles (Haliaeetus leucocephalus). On 7 March 2017, we documented a subadult Bald Eagle depredating an adult Sandhill Crane. Though evidence suggests that Bald Eagles depredate Sandhill Cranes on rare occasion, the process of that depredation has not been described in the scientific literature. The Sandhill Crane effectively defended itself several times against hovering attacks from the Bald Eagle by …


Seasonal Spatial Ecology Of Mountain Lions (Puma Concolor) In The Central Sierra Nevada, Justin A. Dellinger, Eric R. Loft, Ronald C. Bertram, Donald L. Neal, Marc W. Kenyon, Steven G. Torres Jul 2018

Seasonal Spatial Ecology Of Mountain Lions (Puma Concolor) In The Central Sierra Nevada, Justin A. Dellinger, Eric R. Loft, Ronald C. Bertram, Donald L. Neal, Marc W. Kenyon, Steven G. Torres

Western North American Naturalist

Mountain lions (Puma concolor) were captured and radio-collared in the Sierra National Forest between 1983 and 1992 to study the species’ seasonal spatial patterns in a location where migratory mule deer (Odocoileus hemionus) are its primary prey. Some mountain lions displayed seasonal migratory shifts in elevation that mirrored those of their migratory prey; others resided at lower elevations year-round. Home range size for all mountain lions was larger in summer (x– = 231 km2) than in winter (x– = 110 km2), whether or not an individual exhibited seasonal migratory shifts …


Kleptoparasitism Of Harvester Ants By The Giant Kangaroo Rat (Dipodomys Ingens) In The Carrizo Plain, California, Matthew Polvorosa Kline, Jeff A. Alvarez, Nicole Parizeau Jul 2018

Kleptoparasitism Of Harvester Ants By The Giant Kangaroo Rat (Dipodomys Ingens) In The Carrizo Plain, California, Matthew Polvorosa Kline, Jeff A. Alvarez, Nicole Parizeau

Western North American Naturalist

Kangaroo rats are reported to have a mutualistic relationship with harvester ants through facilitation of burrow establishment, creation, and persistence. The relationship can, however, become more complex. We report observations of the giant kangaroo rat (Dipodomys ingens), a state and federally listed endangered species, repeatedly kleptoparasitizing harvester ants from a nearby nest in the Carrizo Plain, California. Though the relationship between kangaroo rats and harvester ants has been studied extensively and is believed to be mutualistic, under certain conditions this relationship can follow an alternate path of parasitism.


Se ha reportado que las ratas canguro mantienen una relación …


Tk1; Journey From The Cytoplasm To The Cell Membrane, Weston Burrup, Kim O'Neill Jul 2018

Tk1; Journey From The Cytoplasm To The Cell Membrane, Weston Burrup, Kim O'Neill

Journal of Undergraduate Research

Thymidine Kinase 1 is an enzyme involved in the synthesis of thymidine triphosphate, an essential DNA building block needed for cell replication. Previous research has shown an upregulated level of TK1 in the serum levels of cancer patients as well as on the cancer cell surface. Due to its’ abnormal presence on the surface of various cancer cells, it is being researched as a potential biomarker for future immunotherapies. In order to be a more reliable biomarker, we need a more in depth understanding of the mechanism of how this protein travels through the cell cytoplasm and eventually attaches on …


Maternal-Fetal Interactions And The Induction Of Preeclampsia By Growth Arrest-Specific 6 (Gas6)/Axl Signaling, Todd Dunaway, Paul Reynolds Jul 2018

Maternal-Fetal Interactions And The Induction Of Preeclampsia By Growth Arrest-Specific 6 (Gas6)/Axl Signaling, Todd Dunaway, Paul Reynolds

Journal of Undergraduate Research

Preeclampsia (PE) is a complicated obstetric complication characterized by increased blood pressure and decreased trophoblast invasion. PE is one of the leading causes of maternal and fetal morbidity and mortality in developed countries. The growth arrest-specific 6 (Gas6) protein is known to induce different responses in cells including prevention of apoptosis and enhanced cell migration and invasion. This protein is secreted in response to growth arrest and it is increased in the serum of PE patients. This discovery suggests a mechanistic role for the Gas6 signaling pathway during PE progression. Gas6 binds to the AXL tyrosine kinase receptor and AXL-mediated …


Conspecific Pollen On Honey Bees And A Chrysomelid Beetle Species Visiting Female Flowers On Nolina Microcarpa (Asparagaceae), William D. Wiesenborn Jul 2018

Conspecific Pollen On Honey Bees And A Chrysomelid Beetle Species Visiting Female Flowers On Nolina Microcarpa (Asparagaceae), William D. Wiesenborn

Western North American Naturalist

Nolina microcarpa (Asparagaceae) is a dioecious monocot shrub found in Arizona, New Mexico, and northern and central Mexico. Leaf rosettes of the species grow in colonies that produce tall inflorescences of small male or female flowers during spring. Dioecious flowering requires pollinating insects to carry pollen from flowers on male colonies to flowers on female colonies. I investigated pollination of female flowers at 12 colonies of N. microcarpa in the Cerbat Mountains in northwestern Arizona during May–June 2017. I examined pollen from male flowers, aspirated insects on female flowers, counted conspecific pollen grains carried by insects, and estimated floral constancies …


Relative Abundance Of Pond Snails In Turnbull National Wildlife Refuge, Washington, And The Spread Of An Invasive Snail, Michele D. Larson, Christina M. Torrieri, A. Ross Black Jul 2018

Relative Abundance Of Pond Snails In Turnbull National Wildlife Refuge, Washington, And The Spread Of An Invasive Snail, Michele D. Larson, Christina M. Torrieri, A. Ross Black

Western North American Naturalist

Freshwater gastropods comprise a diverse and highly imperiled taxonomic group that is threatened by anthropogenic factors including the introduction of nonnative species. We surveyed 10 wetland ponds in Turnbull National Wildlife Refuge to assess the possible expansion of the nonnative European ear snail (Radix auricularia) and to determine the genus richness and relative abundance of native gastropods. D-frame dip nets were used to sample gastropods at 4 locations in each pond. Six genera of gastropods were found, including R. auricularia in 6 ponds where it was not previously known. Radix auricularia is now the dominant genus in Middle …