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Employee Skill Tracking With Open Badges, Kyle Clements Oct 2018

Employee Skill Tracking With Open Badges, Kyle Clements

Instructional Psychology and Technology Graduate Student Projects

The Harold B. Lee Library (HBLL) multimedia lab at BYU serves university students and employees by checking out library equipment, providing technical assistance for various software products, and teaching patrons how to use these products in library software courses. The goal of this project was to create a training program to guide multimedia lab employees in obtaining the skills necessary to fulfill these roles and provide multimedia lab management with a means of tracking employee skills. Open Badges are used to define and recognize specific competencies for each skill within this program.

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Long-Term Monitoring Of Scripps's Murrelet And Guadalupe Murrelet At San Clemente Island, California: Evaluation Of Baseline Data In 2012–2016, Darrell L. Whitworth, Harold R. Carter, Michael W. Parker, Franklin Gress, Melissa Booker Oct 2018

Long-Term Monitoring Of Scripps's Murrelet And Guadalupe Murrelet At San Clemente Island, California: Evaluation Of Baseline Data In 2012–2016, Darrell L. Whitworth, Harold R. Carter, Michael W. Parker, Franklin Gress, Melissa Booker

Western North American Naturalist

San Clemente Island (SCI) supports one of the smallest Scripps’s Murrelet (Synthliboramphus scrippsi; SCMU) colonies in the world, and perhaps the only colony of Guadalupe Murrelets (S. hypoleucus; GUMU) in California. In 2012–2016, the U.S. Navy sponsored development of a long-term murrelet monitoring program at SCI that utilized nocturnal spotlight surveys, night-lighting at-sea captures, and nest monitoring. Standardized spotlight survey transects were established in nearshore waters off breeding areas at Seal Cove and southeast SCI (SESCI). Baseline mean spotlight counts were 29 ± 15 murrelets (n = 31) at Seal Cove in 2013–2016 and 21 ± 10 …

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Connecting Island Communities On A Global Scale: Case Studies In Island Biosecurity, Juliana Matos, Annie Little, Keith Broome, Euan Kennedy, Frederico A. Méndez-Sánchez, Mariam Latofski-Robles, Robyn Irvine, Chris Gill, Aurora Espinoza, Gregg Howald, Katrina Olthof, Morgan Ball, Christina L. Boser Oct 2018

Connecting Island Communities On A Global Scale: Case Studies In Island Biosecurity, Juliana Matos, Annie Little, Keith Broome, Euan Kennedy, Frederico A. Méndez-Sánchez, Mariam Latofski-Robles, Robyn Irvine, Chris Gill, Aurora Espinoza, Gregg Howald, Katrina Olthof, Morgan Ball, Christina L. Boser

Western North American Naturalist

Invasive alien species represent one of the greatest threats to island ecosystems and the unique species that inhabit them. In many instances, eradication or control programs for invasive alien species have effectively curtailed the ongoing loss of biodiversity on islands. Prevention is a more proactive and cost-effective approach, however, and is an emerging global priority in the conservation of island ecosystems. Island biosecurity programs attempt to prevent the introduction and establishment of invasive alien species on islands and dictate actions when an invasive species is detected. Targeted and robust collaboration efforts among the global island community on biosecurity advances and …

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Distribution And Characterization Of Wild Bee Nesting Sites On San Clemente Island, California Channel Islands, C. Sheena Sidhu, Erin E. Wilson Rankin Oct 2018

Distribution And Characterization Of Wild Bee Nesting Sites On San Clemente Island, California Channel Islands, C. Sheena Sidhu, Erin E. Wilson Rankin

Western North American Naturalist

Wild bees provide the essential ecosystem service of pollination, which may be particularly important in island systems that host rare and endangered plants. Identifying and protecting nesting habitat of wild bees is critical for successfully conserving these pollinators and their pollination services, but characterization and delimitation of nesting habitat receives relatively little attention in the literature compared to description of floral interactions. Such data on wild bee nesting is necessary for creating effective conservation strategies that benefit the stability of ecosystem services, as well as promote the populations of both pollinators and plants. On San Clemente Island, we identified the …

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Horizon Scanning: Survey And Research Priorities For Coastal And Marine Systems Of The Northern Channel Islands, California, Mary G. Gleason, Jennifer E. Caselle, Chris Caldow, Russell Galipeau, Walter Heady, Corinne Laverty, Annie Little, David Mazurkiewicz, Eamon O'Byrne, Dirk Rosen, Stephen Whitaker Oct 2018

Horizon Scanning: Survey And Research Priorities For Coastal And Marine Systems Of The Northern Channel Islands, California, Mary G. Gleason, Jennifer E. Caselle, Chris Caldow, Russell Galipeau, Walter Heady, Corinne Laverty, Annie Little, David Mazurkiewicz, Eamon O'Byrne, Dirk Rosen, Stephen Whitaker

Western North American Naturalist

Historical marine ecology provides information on past ocean conditions and community structure that can inform current conservation and management. In an era of rapid global ocean changes, it is critical that managers and scientists ensure sufficient documentation of past and present conditions of resources they manage or study. Documenting, archiving, and preserving historic and contemporary data will provide their colleagues in the future with more information to make robust science-based management decisions. Using a workshop approach, we identified research and archiving priorities to enhance documentation of the past and present conditions of coastal and marine ecosystems of the northern Channel …

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The Introduced Argentine Ant (Linepithema Humile) On The California Channel Islands: Distribution And Patterns Of Spread, Christina L. Boser, Korie Merrill, Robert N. Fisher, Ida Naughton, David A. Holway Oct 2018

The Introduced Argentine Ant (Linepithema Humile) On The California Channel Islands: Distribution And Patterns Of Spread, Christina L. Boser, Korie Merrill, Robert N. Fisher, Ida Naughton, David A. Holway

Western North American Naturalist

The Argentine ant (Linepithema humile) is a widespread and abundant introduced species that disrupts ecosystems throughout its introduced range. This invader was inadvertently introduced to Santa Catalina, San Clemente, Santa Cruz, and San Nicolas Islands at various points during the past century but currently appears to be absent from the remaining Channel Islands. Multiple spatially disjunct infestations on each invaded island individually range in size from <500 m2 to >427 ha and encompass a variety of habitats, including large areas that are entirely dominated by native perennial vegetation. The existence of multiple infestations on individual islands suggests that inadvertent …

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Avian And Skunk Predation Of Ashy Storm-Petrels At Santa Cruz Island, California, William R. Mciver, Harold R. Carter, A. Laurie Harvey, David M. Mazurkiewicz, Jim A. Howard, Paige L. Martin, John W. Mason Oct 2018

Avian And Skunk Predation Of Ashy Storm-Petrels At Santa Cruz Island, California, William R. Mciver, Harold R. Carter, A. Laurie Harvey, David M. Mazurkiewicz, Jim A. Howard, Paige L. Martin, John W. Mason

Western North American Naturalist

Avian and mammalian predation of Ashy Storm-Petrels (Oceanodroma homochroa) were documented during monthly nest monitoring in 1995–1998 and 2005–2015 and once-per-year monitoring in 1999–2004 at 5 colonies at Santa Cruz Island, California. Throughout the study period, predation by Barn Owls (Tyto alba) was documented at the sea cave colonies, and at Orizaba Rock, and likely contributed to reduced breeding success at Bat Cave in 1995–1997. Recent predation by Common Ravens (Corvus corax) caused reduced breeding success each year in 2012–2015 at Orizaba Rock (35%–48%) and in 2013 and 2015 at Bat Cave (49%–52%). Raven …

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Equipping Tomorrow's Historical Ecologist: Priorities For Documenting Conditions Of The Terrestrial Fauna Of Santa Cruz Island, California, Christina L. Boser, T. Scott Sillett, Paul W. Collins, Kathryn R. Faulkner, W. Chris Funk, Cameron K. Ghalambor, Lyndal Laughrin, Gregory B. Pauly, Jeanne M. Robertson, Robyn Shea, Winston Vickers Oct 2018

Equipping Tomorrow's Historical Ecologist: Priorities For Documenting Conditions Of The Terrestrial Fauna Of Santa Cruz Island, California, Christina L. Boser, T. Scott Sillett, Paul W. Collins, Kathryn R. Faulkner, W. Chris Funk, Cameron K. Ghalambor, Lyndal Laughrin, Gregory B. Pauly, Jeanne M. Robertson, Robyn Shea, Winston Vickers

Western North American Naturalist

Historical ecological research provides valuable insights for contemporary conservation management. Gaps in historical records, however, can limit the utility of that research. Future conservationists may therefore find themselves disadvantaged by the current societal trend of underinvestment in systematic collection of museum specimens and natural history information. To reduce that risk, we asked what managers and scientists could do today to better document the past and present conditions of Santa Cruz Island, California, as a means to improve both contemporary and future conservation. We focused our inquiry on the island’s terrestrial fauna, which includes numerous taxa of conservation concern. Here we …

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Yes We Can! Exciting Progress And Prospects For Controlling Invasives On Islands And Beyond, Daniel Simberloff, Brad Keitt, David Will, Nick Holmes, Erin Pickett, Piero Genovesi Oct 2018

Yes We Can! Exciting Progress And Prospects For Controlling Invasives On Islands And Beyond, Daniel Simberloff, Brad Keitt, David Will, Nick Holmes, Erin Pickett, Piero Genovesi

Western North American Naturalist

Eradication and maintenance management of island invasive species have long histories, and incremental improvements of existing technologies plus occasional novel approaches have led to more challenging targets and increased success rates in certain categories. Many nonnative mammals have been eradicated from islands, as have several nonnative birds, insects, and plants. Hundreds of rat populations have been eliminated, with a success rate over 80%, and islands over 10,000 ha are now feasible targets. Mouse eradication has proven more challenging, but aerial broadcast of anticoagulant toxins has led to increased success. Carnivore eradication—especially of feral housecats and foxes—has been frequently attempted with …

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Status Of Ashy Storm-Petrel Breeding Colonies At Santa Cruz Island, California, 1912–1998, Harry R. Carter, William R. Mciver, Gerard J. Mcchesney, Darrell L. Whitworth, John R. Gilardi Oct 2018

Status Of Ashy Storm-Petrel Breeding Colonies At Santa Cruz Island, California, 1912–1998, Harry R. Carter, William R. Mciver, Gerard J. Mcchesney, Darrell L. Whitworth, John R. Gilardi

Western North American Naturalist

We summarized historical knowledge from 1912 to 1998 of 13 known breeding colonies of Ashy Storm-Petrels (Oceanodroma homochroa) at Santa Cruz Island, California. Breeding was first reported by naturalists at Painted Cave (1912), Scorpion Rocks (1928), and Orizaba Rock (1937). During early seabird colony surveys of the Channel Islands, breeding was also noted at Cavern Point Cove Caves (1970), Diablo Rocks (1976), and Gull Island (1977). During 1991–1998, more extensive surveys of nearly all sea caves and offshore rocks documented additional breeding at Bat Cave, Cave of the Birds’ Eggs, Del Mar Cove Cave, Del Mar Rock, Dry …

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Cip2a Immunosensor Comprised Of Vertically-Aligned Carbon Nanotube Interdigitated Electrodes Towards Point-Of-Care Oral Cancer Screening, Shaowei Ding, Suprem R. Das, Benjamin J. Brownlee, Kshama Parate, Taylor Davis, Loreen R. Stromberg, Edward K.L. Chan, Joseph Katz, Brian D. Iverson, Jonathan C. Claussen Oct 2018

Cip2a Immunosensor Comprised Of Vertically-Aligned Carbon Nanotube Interdigitated Electrodes Towards Point-Of-Care Oral Cancer Screening, Shaowei Ding, Suprem R. Das, Benjamin J. Brownlee, Kshama Parate, Taylor Davis, Loreen R. Stromberg, Edward K.L. Chan, Joseph Katz, Brian D. Iverson, Jonathan C. Claussen

Faculty Publications

Vertically aligned carbon nanotube array (VANTA) coatings have recently garnered much attention due in part to their unique material properties including light absorption, chemical inertness, and electrical conductivity. Herein we report the first use of VANTAs grown via chemical vapor deposition in a 2D interdigitated electrode (IDE) footprint with a high height-to-width aspect ratio (3:1 or 75:25 µm). The VANTA-IDE is functionalized with an antibody (Ab) specific to the human cancerous inhibitor PP2A (CIP2A)—a alivary oncoprotein that is associated with a variety of malignancies such as oral, breast, and multiple myeloma cancers. The resultant immunosensor is capable of detecting CIP2A …

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Scripps's Murrelet At San Miguel Island, California: Status Of A Small Population At The Northwest Limit Of The Breeding Range, Darrell L. Whitworth, Harry R. Carter Oct 2018

Scripps's Murrelet At San Miguel Island, California: Status Of A Small Population At The Northwest Limit Of The Breeding Range, Darrell L. Whitworth, Harry R. Carter

Western North American Naturalist

San Miguel Island (SMI) and its adjacent islets, Prince Island and Castle Rock, support a small population of Scripps’s Murrelets (Synthliboramphus scrippsi) at the northwest limit of their breeding range. In 1994–1996, 2004, 2007, and 2015, we conducted at-sea surveys and nest searches to determine the breeding distribution and population size of the Scripps’s Murrelet at the SMI group. During at-sea vocal surveys in 1994–1996, murrelet calls were heard at 4 stations around Prince Island (43–167 calls), 2 stations off Castle Rock (6 calls and 80 calls), and 4 stations off northeast SMI (5–123 calls), but none were …

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Using Machine Learning To Accurately Predict Ambient Soundscapes From Limited Data Sets, Katrina Lynn Pedersen Oct 2018

Using Machine Learning To Accurately Predict Ambient Soundscapes From Limited Data Sets, Katrina Lynn Pedersen

Theses and Dissertations

The ability to accurately characterize the soundscape, or combination of sounds, of diverse geographic areas has many practical implications. Interested parties include the United States military and the National Park Service, but applications also exist in areas such as public health, ecology, community and social justice noise analyses, and real estate. I use an ensemble of machine learning models to predict ambient sound levels throughout the contiguous United States. Our data set consists of 607 training sites, where various acoustic metrics, such as overall daytime L50 levels and one-third octave frequency band levels, have been obtained. I have data for …

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Molecular Characterization Of Penstemon Scariosus (Plantaginaceae), Mikel R. Stevens Oct 2018

Molecular Characterization Of Penstemon Scariosus (Plantaginaceae), Mikel R. Stevens

Journal of Undergraduate Research

The objectives of this project: Our objective was “to develop an understanding of the genetic structure of the varieties within P. scariosus. With a specific intent to test the hypothesis that P. scariosus var. albifluvis is distinct within P. scariosus” while mentoring no less than four undergraduate students. We have meet and exceeded the academic objectives proposed and then funded by this MEG as attested to in the following paragraphs and attachments to this report. As a result of our most excellent success in this project we have four additional students who are volunteering on the project. It is our …

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Environmental Crime And Civilization: Identification; Impacts; Threats And Rapid Response – June 2018, Lynn Rhodes Oct 2018

Environmental Crime And Civilization: Identification; Impacts; Threats And Rapid Response – June 2018, Lynn Rhodes

Comparative Civilizations Review

No abstract provided.

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Niccolo Leo Caldararo, Big Brains And The Human Superorganism: Why Special Brains Appear In Hominids And Other Social Animals. Rowman & Littlefield, 2017, Andrew Targowski Oct 2018

Niccolo Leo Caldararo, Big Brains And The Human Superorganism: Why Special Brains Appear In Hominids And Other Social Animals. Rowman & Littlefield, 2017, Andrew Targowski

Comparative Civilizations Review

No abstract provided.

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48th Annual Iscsc Conference Oct 2018

48th Annual Iscsc Conference

Comparative Civilizations Review

No abstract provided.

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Comparative Civilizations Review No. 79 Full Issue, Comparative Civilizations Review Oct 2018

Comparative Civilizations Review No. 79 Full Issue, Comparative Civilizations Review

Comparative Civilizations Review

No abstract provided.

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Nanofluidic Applications Of Silica Membranes, John Michael Stout Oct 2018

Nanofluidic Applications Of Silica Membranes, John Michael Stout

Theses and Dissertations

This work presents membrane development applicable in nanofluidic devices. These membranes can also be termed suspended thin films, supported on two or more edges. I first discuss motivation and background for developing these structures. Then I derive the formative principles for nanofluidic systems. Following the derivation of the Navier-Stokes and Washburn equations, I discuss applying these theories to planar nanofluidic capillaries and finish the derivation by discussing the forces that drive liquid flow in nanochannels. I next discuss the membrane development process, starting with my work in static height traps, and develop the concept of analyzing nanoparticles using suspended membranes. …

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Development And Use Of Lipidomics And Proteomics Methods To Identify And Measure Pro-Survival Metabolic Pathways In Cancer, Monique Merilyn Speirs Oct 2018

Development And Use Of Lipidomics And Proteomics Methods To Identify And Measure Pro-Survival Metabolic Pathways In Cancer, Monique Merilyn Speirs

Theses and Dissertations

Throughout society’s continual war against cancer, we have attempted pharmacological intervention only to find that tumors develop modes of resistance. It is well known that genetics play an integral role in cancer. Technological advances have greatly improved our ability to study cancer biochemistry beyond the genome by measuring changes in the expression and activity of RNA, proteins, and lipids in experimental models and human patients. As our techniques and technology to perform cancer research progresses, it is becoming more evident that cancer cells develop stress tolerance mechanisms at multiple levels within the central dogma, including altering mRNA expression, enzyme concentrations, …

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Thermochemical Conversion Of Biomass: Detailed Gasification And Near-Burner Co-Firing Measurements, Jacob B. Beutler Oct 2018

Thermochemical Conversion Of Biomass: Detailed Gasification And Near-Burner Co-Firing Measurements, Jacob B. Beutler

Theses and Dissertations

An increasing emphasis on mitigating global climate change (global warming) over the last few decades has created interest in a broad range of sustainable or alternative energy systems to replace fossil fuel combustion. Biomass, when harvested responsibly, is a renewable fuel with many uses in replacing fossil fuels. Cofiring biomass with coal in traditional large-scale coal power plants represents one of the lowest risk, least costly, near-term methods of CO2 mitigation. Simultaneously, it is one of the most efficient and inexpensive uses of biomass. Alternatively, biomass can be transformed into useful products through gasification to produce clean syngas for …

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Distribution, Habitat, And Population Size Of Island Night Lizards On San Nicolas Island, California, Charles A. Drost, Gary M. Fellers, Thomas G. Murphey, Patrick M. Kleeman, Brian J. Halstead, Ryan P. O'Donnell Sep 2018

Distribution, Habitat, And Population Size Of Island Night Lizards On San Nicolas Island, California, Charles A. Drost, Gary M. Fellers, Thomas G. Murphey, Patrick M. Kleeman, Brian J. Halstead, Ryan P. O'Donnell

Western North American Naturalist

The Island Night Lizard (Xantusia riversiana) was removed from the federal list of threatened species in May 2014. This strongly differentiated species is endemic to 3 of the southern California Channel Islands—San Clemente, San Nicolas, and Santa Barbara. Suitable habitat for Island Night Lizards is extensive on San Clemente Island, and the species is abundant there. Habitat is limited and fragmented, however, on San Nicolas Island and small Santa Barbara Island. Bringing together extensive field surveys and mark-recapture sampling, we synthesize available data for Island Night Lizards on San Nicolas Island and calculate population and density estimates for …

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Comparison Of Estimators For Monitoring Long-Term Population Trends In Deer Mice, Peromyscus Maniculatus, On The California Channel Islands, Catherin A. Schwemm, Charles A. Drost, John L. Orrock, Timothy J. Coonan, Thomas R. Stanley Sep 2018

Comparison Of Estimators For Monitoring Long-Term Population Trends In Deer Mice, Peromyscus Maniculatus, On The California Channel Islands, Catherin A. Schwemm, Charles A. Drost, John L. Orrock, Timothy J. Coonan, Thomas R. Stanley

Western North American Naturalist

Capture-recapture methods are commonly used to estimate abundance and density of wild animal populations. Although a variety of sophisticated analytical techniques are available to evaluate capture-recapture data, vertebrate monitoring programs often lack the resources (e.g., time, personnel, and/or analytical expertise) to apply these methods. As an alternative, simple population indices, such as counts of unique individuals, may provide sufficient information to detect meaningful changes in population size. In this study we investigated whether a population index, easily generated from mark-recapture data under all conditions, might be used to provide valid ecological information for managers interested in long-term population trends of …

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Evolution Of The Photopigments In The Largest And Most Complex Terrestrial Animal Eyes, Seth M. Bybee Sep 2018

Evolution Of The Photopigments In The Largest And Most Complex Terrestrial Animal Eyes, Seth M. Bybee

Journal of Undergraduate Research

Evaluation of how well the academic objectives of the proposal were met The proposal had three main aims: 1). Produce a phylogenetic estimate of aeshnid dragonfly evolution from transcriptomes. 2). Use the phylogeny from Aim I and the photopigments isolated from the transcriptome data to examine the evolution of aeshnid color visual systems and 3) Using independent and complimentary methods (in situ hybridization, qPCR and transcriptome data) to examine both the distribution and level of expression for each photopigment for one focal species (Anax junius). Goals one and two were both successful, however goal three became bogged down and only …

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The Effect Of Nr4a3 On Functional Beta Cell Mass, Jeffery S. Tessem Sep 2018

The Effect Of Nr4a3 On Functional Beta Cell Mass, Jeffery S. Tessem

Journal of Undergraduate Research

The academic objectives of this Mentoring Environment Grant (MEG) were to 1) train undergraduate students in the scientific process to prepare them in careers as physicians and scientists, and 2) to determine if the in vitro phenotypes observed in Nr4a3 deficient beta cell lines are translatable to the Nr4a3 KO mouse. . We were successful in accomplishing these objectives, as evidenced by the training of four undergraduate students, presentation of research and local and national meetings, and initial preparation of a manuscript reporting our findings. While we did have difficulty breeding these mice, this study gives us the basis of …

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Using Non Stem-Cells To Understand Early Tumor Growth, Jake Hogan, Heiko Enderling, Joel Brown, Robert A. Gatenby Sep 2018

Using Non Stem-Cells To Understand Early Tumor Growth, Jake Hogan, Heiko Enderling, Joel Brown, Robert A. Gatenby

Library/Life Sciences Undergraduate Poster Competition 2018

Tumors of similar size and shape can exhibit different responses to the same treatment. Targeted therapy aims to better treat these tumors by classifying them according to genotypic traits. A better understanding of how tumor traits such as non-stem cells influence tumor growth could improve targeted therapy. We hypothesize that the production of non-stem cells may aid tumor growth in avascular tumors (tumors lacking blood vessels).

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Spatial And Temporal Fishing Patterns At The Outer Banks Of The Southern California Bight, Daniel J. Pondella Ii, Matthew J. Robart, Jeremy T. Claisse, Jonathan P. Williams, Chelsea M. Williams, Amanda J. Zellmer, Susan E. Piacenza Sep 2018

Spatial And Temporal Fishing Patterns At The Outer Banks Of The Southern California Bight, Daniel J. Pondella Ii, Matthew J. Robart, Jeremy T. Claisse, Jonathan P. Williams, Chelsea M. Williams, Amanda J. Zellmer, Susan E. Piacenza

Western North American Naturalist

We analyzed recreational and commercial catch records from 1980 to 2009 for the fishing blocks associated with the 5 southern offshore banks of the Southern California Bight (Cortes Bank, Tanner Bank, Northeast Bank, Cherry Ridge, and Garrett Ridge). Recreational fishers and divers targeted pelagic taxa (e.g., tunas and Yellowtail) but instead landed more benthic taxa (e.g., rockfishes, Ocean Whitefish, and California Sheephead). For the commercial fisheries, pelagics (Bluefin Tuna, Albacore, Skipjack, Yellowfin Tuna, Pacific Bonito, and Swordfish) were the most important taxa and represented a significant proportion (8%–27%) of the landings of these fishes in the bight. These fishes were …

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Glutathione-Mediated Oxidative Response In Uvb-Irradiated Hacat Cells, Patrick Badger, Dr. Jason Hansen Sep 2018

Glutathione-Mediated Oxidative Response In Uvb-Irradiated Hacat Cells, Patrick Badger, Dr. Jason Hansen

Journal of Undergraduate Research

Ultraviolet light (UV) plays a critical role in the etiology of skin cancer, contributing to over 95% of both melanoma and non-melanoma diagnoses. It consists of several wavelengths, among which UVB is the most harmful, causing inflammation associated with sunburn and constituting the principal cause of skin cancer-related death.

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Sequencing Complete Genome Of Cutthroat Trout Subspecies For Purposes Of Improving Classification Of Cutthroat Trout Species, Emily Brown, Dr. Dennis Shiozawa Sep 2018

Sequencing Complete Genome Of Cutthroat Trout Subspecies For Purposes Of Improving Classification Of Cutthroat Trout Species, Emily Brown, Dr. Dennis Shiozawa

Journal of Undergraduate Research

Since the 1800s, biologists have studied cutthroat trout native to Western North America. Their early work and classification were based on the standards of the day: meristics, the observation and counting of physical features, and morphology. Further improvements came through later studies that added geographic distribution to their phylogenetic classification. Without the foresight of DNA genotyping methods, “traditional taxonomic assessments often failed to accurately capture phylogenetic diversity.” Many discrepancies have since arisen as genetic methods shed new light on cutthroat trout subspecies phylogenies. Current mtDNA work has identified errors in historical classifications of several of these subspecies in the Colorado, …

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An Efficient Method For Generating Conditional Cell Lines For Cellular And Molecular Research, Mark Roth, Dr. Jonathan Alder Sep 2018

An Efficient Method For Generating Conditional Cell Lines For Cellular And Molecular Research, Mark Roth, Dr. Jonathan Alder

Journal of Undergraduate Research

Conditional cell lines are widely used throughout biochemical research. The basic concept is that you can delete a gene you are studying upon the addition of a drug, to cells in culture, and then use the knockout cells to study the genes effects. This process has been facilitated by the advent of the CRISPR/Cas9 system that allows a broad range of genome editing in living cells. Cas9 introduces a double strand break in DNA at a site specified by the CRISPR (guide RNA), and by changing the CRISPR sequence researchers can introduce these double strand breaks almost wherever they want. …

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