Digital Commons Network^™

An Evaluation Of Avian Influenza Virus Whole-Genome Sequencing Approaches Using Nanopore Technology, Hon S. Ip, Sarah Uhm, Mary Lea Killian, Mia K. Torchetti

United States Department of Agriculture Wildlife Services: Staff Publications

As exemplified by the global response to the SARS-CoV-2 pandemic, whole-genome sequencing played an important role in monitoring the evolution of novel viral variants and provided guidance on potential antiviral treatments. The recent rapid and extensive introduction and spread of highly pathogenic avian influenza virus in Europe, North America, and elsewhere raises the need for similarly rapid sequencing to aid in appropriate response and mitigation activities. To facilitate this objective, we investigate a next-generation sequencing platform that uses a portable nanopore sequencing device to generate and present data in real time. This platform offers the potential to extend in-house sequencing …

Non-Aggressive Competition Between Males Of Srilankametrus Yaleensis (Kovařík Et Al., 2019) (Scorpionidae), And Other Types Of Agonistic Behavior Observed In Scorpions, Victoria Tang

Euscorpius

A peculiar intraspecific agonistic behavior involving a non-aggressive physical combat is reported between the adult males of Srilankametrus yaleensis (Kovařík et al., 2019) (Scorpionidae: Heterometrinae). The adult males were observed to resort to a ritualized and relatively gentle way for strength demonstration. The combat is characterized by lateral spreading of pedipalps, chelicerae-to-chelicerae collision, and entanglement of metasomal segments. This behavior is hereby considered a form of an intrasexual combat defined as the “arm-span competition”. It is hypothesized to be beneficial for solving territorial and/ or sexual competitions while avoiding unnecessary mortality which could pose adverse impact to the natural populations. …

Methylene Blue Inhibits Cromakalim-Activated K+ Currents In Follicle-Enclosed Oocytes, Dmytro Isaev, Keun-Hang Susan Yang, Georg Petroianu, Dietrich Ernst Lorke, Murat Oz

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The effects of methylene blue (MB) on cromakalim-induced K⁺ currents were investigated in follicle-enclosed Xenopus oocytes. In concentrations ranging from 3–300 μM, MB inhibited K⁺ currents (IC₅₀: 22.4 μM) activated by cromakalim, which activates K_ATP channels. MB inhibited cromakalim-activated K⁺ currents in a noncompetitive and voltage-independent manner. The respective EC₅₀ and slope values for cromakalim-activation of K⁺ currents were 194 ± 21 µM and 0.91 for controls, and 206 ± 24 µM and 0.87 in the presence of 30 μM MB. The inhibition of cromakalim-induced K⁺ currents by MB was not …

Oregon State Rank Assessment For Pacific Brook Lamprey (Lampetra Pacifica), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Pacific Brook Lamprey (Lampetra pacifica) using NatureServe methodology, 2023.

Lychas Jakli Sp. N. (Scorpiones: Buthidae) From Indonesia, František Kovařík

Euscorpius

A new species Lychas jakli sp. n. (Scorpiones: Buthidae) is described from Indonesia, fully illustrated with color photos showing its morphology and habitus. A new synonymy is proposed: Lychas kotao Lourenço, 2020 = Lychas mucronatus (Fabricius, 1798), syn. n.

Hypotheses Related To Anting By Birds, Helena Flores

2023 REYES Proceedings

“Anting” is a widespread behavior among bird species and mammals. Due to the various characteristics of this behavior, numerous hypotheses have been proposed to explain it. This behavior can be classified as “active”, in which birds take a bill full of ants and rub them into their feathers, or “passive” when birds let ants wander through their plumage. The hypotheses state that anting may be performed as an (1) antiparasitic behavior, for (2) feather grooming, as a way of (3) food preparation or to (4) decrease skin irritation during molt, even as a (5) sensory stimulation tool. This review compiles …

Rapid Growth In Late Cretaceous Sea Turtles Reveals Life History Strategies Similar To Extant Leatherbacks, Laura E. Wilson

Geosciences Faculty Publications

Modern sea turtle long bone osteohistology has been surprisingly well-studied, as it is used to understand sea turtle growth and the timing of life history events, thus informing conservation decisions. Previous histologic studies reveal two distinct bone growth patterns in extant sea turtle taxa, with Dermochelys (leatherbacks) growing faster than the cheloniids (all other living sea turtles). Dermochelys also has a unique life history compared to other sea turtles (large size, elevated metabolism, broad biogeographic distribution, etc.) that is likely linked to bone growth strategies. Despite the abundance of data on modern sea turtle bone growth, extinct sea turtle osteohistology …

The Effects Of Chemical Secretions By Millipedes On Anting Behaviour In Birds And Other Animals, Theo Fraser

2023 REYES Proceedings

Birds perform a behavior known as anting in which they actively rub ants in their feathers or allow ants to crawl over their bodies. Several hypotheses have been proposed to explain this behavior, but no one hypothesis can account for all cases of anting that have been observed. Anting behavior has been recorded in species other than birds including primates, and substances other than ants such as millipedes may be used. This paper explores the use of millipedes in anting by birds, capuchin monkeys and lemurs. The three hypotheses supported by these studies include 1) anting serves as food preparation …

Environmental Transmission Of Influenza A Virus In Mallards, Kim M. Pepin, Clinton B. Leach, Nicole L. Barrett, Jeremy W. Ellis, Kaci K. Vandalen, Colleen T. Webb, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

Abstract

Influenza A viruses present a major challenge for animal and human health. They circulate widely in wild waterfowl and frequently spillover into poultry, emphasizing the need for risk-based surveillance in wild birds and an understanding of the relative importance of different transmission mechanisms. We addressed this objective with a replicated (N = 6) experimental infection study in which we serially exposed eight cohorts of four naïve contact mallards to an experimentally infected mallard and a shared water pool. Viral concentration in the water was a better predictor of transmission than several direct measures of viral shedding in the …

Changes In Wild Pig (Sus Scrofa) Relative Abundance, Crop Damage, And Environmental Impacts In Response To Control Efforts, Joseph W. Treichler, Kurt C. Vercauteren, Charles R. Taylor, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Background

As the population and range of wild pigs (Sus scrofa) continue to grow across North America, there has been an increase in environmental and economic damages caused by this invasive species, and control efforts to reduce damages have increased concomitantly. Despite the expanding impacts and costs associated with population control of wild pigs, the extent to which wild pig control reduces populations and diminishes environmental and agricultural damages are rarely quantified.The goal of this study is to quantify changes in wild pig relative abundance and subsequent changes in damages caused by invasive wild pigs in response to …

Echinococcus Species Infections Among Wild Canids In Pennsylvania, Usa, Kayla Garrett, Justin Brown, Ryan K. A. Grunert, Jonathan Hunte, Mark G. Ruder, Kyle Van Why, Michael J. Yabsley, Christopher A. Cleveland

United States Department of Agriculture Wildlife Services: Staff Publications

Echinococcus species are zoonotic tapeworms that can impact the health of wildlife, domestic animals, livestock, and humans. Two species of interest in North America are Echinococcus multilocularis and Echinococcus canadensis (Echinococcus granulosus sensu lato). The primary wildlife definitive hosts for E. multilocularis and E. canadensis are similar, including red foxes (Vulpes vulpes), gray foxes (Urocyon cinereoargenteus), coyotes (Canis latrans), and wolves (Canis lupus). These two Echinococcus spp. use different intermediate hosts, including small mammals for E. multilocularis and artiodactylids for E. canadensis. Although historically absent from …

Understory Arthropod Diversity In A Mixed Dryland Ecosystem, Hawai‘I, Matthew J. Madeiros, William P. Haines, Clare E. Aslan, Aaron B. Shiels, Asa Aue, Christina T. Liang

United States Department of Agriculture Wildlife Services: Staff Publications

Tropical drylands are among the most threatened ecosystems worldwide, and fewer than 10% of tropical dry forests in Hawai‘i remain intact. Terrestrial arthropods comprise most of the endemic fauna on many islands, including Hawai‘i, where they provide ecosystem services such as pollination, decomposition, and a reliable prey base for predators. The objective of our study was to measure understory arthropod diversity and relative abundances in two common habitat types within dryland Hawai‘i—a non-native grassland, dominated by Cenchrus setaceus (fountain grass) with few native shrubs, and a native woodland, dominated by Metrosideros polymorpha (‘ōhi‘a). We used generalized additive models to examine …

Evaluation Of Roost Culling As A Management Strategy For Reducing Invasive Rose‑Ringed Parakeet (Psittacula Krameri) Populations, C. Janes Anderson, Leonard A. Brennan, William P. Bukoski, Steven C. Hess, Clayton D. Hilton, Aaron B. Shiels, Shane Siers, Bryan M. Kluever, Page E. Klug

United States Department of Agriculture Wildlife Services: Staff Publications

Rose-ringed parakeets (Psittacula krameri) are one of the most widespread invasive avian species worldwide. This species was introduced to the island of Kaua‘i, Hawai‘i, USA, in the 1960s. The rapidly increasing population has caused substantial economic losses in the agricultural and tourism industries. We evaluated the efficacy of a roost culling program conducted by an independent contractor from March 2020 to March 2021. We estimated island-wide minimum abundance was 10,512 parakeets in January 2020 and 7,372 in April 2021. Over 30 nights of culling at four roost sites, approximately 6,030 parakeets were removed via air rifles with 4,415 …

Social Network Analysis Of White-Tailed Deer Scraping Behavior: Implications For Disease Transmission, Michael E. Egan, Kim M. Pepin, Justin Fischer, Scott R. Hygnstrom, Kurt C. Vercauteren, Guillaume Bastille-Rousseau

United States Department of Agriculture Wildlife Services: Staff Publications

Host contact structure affects pathogen transmission in host populations, but many measures of host contact do not distinguish contacts that are relevant to pathogen transmission from those that are not. Scrapes are sites for chemical communication by white-tailed deer (Odocoileus virginianus) during the breeding season and potential sites of transmission of prions, the causative agent of chronic wasting disease (CWD). Scrape-related behaviors vary in their probability of transmitting prions to or from the environment, suggesting that behavior be combined with contact structure to better reflect potential heterogeneity in prion transmission at scrapes. We recorded visits and behaviors by …

Disease Progression And Serological Assay Performance In Heritage Breed Pigs Following Brucella Suis Experimental Challenge As A Model For Naturally Infected Feral Swine, Vienna R. Brown, Ryan S. Miller, Courtney F. Bowden, Timothy J. Smyser, Nicholas A. Ledesma, Aim E. Hartwig, Paul Gordy, Aaron M. Anderson, Stephanie M. Porter, Kate Alexander, Zane Gouker, Tom Gidlewski, Richard A. Bowen, Angela M. Bosco-Lauth

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive feral swine (Sus scrofa) are one of the most important wildlife species for disease surveillance in the United States, serving as a reservoir for various diseases of concern for the health of humans and domestic animals. Brucella suis, the causative agent of swine brucellosis, is one such pathogen carried and transmitted by feral swine. Serology assays are the preferred field diagnostic for B. suis infection, as whole blood can be readily collected and antibodies are highly stable. However, serological assays frequently have lower sensitivity and specificity, and few studies have validated serological assays for B. suis in feral swine. …

Evaluation Of The Effect Of Hydrated Lime On The Scavenging Of Feral Swine (Sus Scrofa) Carcasses And Implications For Managing Carcass-Based Transmission Of African Swine Fever Virus, Courtney F. Bowden, James Grinolds, Gregory Franckowiak, Loma Mccallister, Joe M. Halseth, Matthew Cleland, Travis Guerrant, Michael J. Bodenchuk, Robert Miknis, Michael C. Marlow, Vienna R. Brown

United States Department of Agriculture Wildlife Services: Staff Publications

African swine fever (ASF) is a devastating hemorrhagic disease marked by extensive morbidity and mortality in infected swine. The recent global movement of African swine fever virus (ASFV) in domestic and wild swine (Sus scrofa) populations has initiated preparedness and response planning activities within many ASF-free countries. Within the US, feral swine are of utmost concern because they are susceptible to infection, are wide-spread, and are known to interact with domestic swine populations. African swine fever virus is particularly hardy and can remain viable in contaminated carcasses for weeks to months; therefore, carcass-based transmission plays an important role in the …

Perturbations Highlight Importance Of Social History In Parakeet Rank Dynamics, Annemarie Van Der Marel, Xavier Francis, Claire L. O'Connell, Cesar O. Estien, Chelsea Carminito, Virginia Darby Moore, Nickolas Lormand, Bryan M. Kluever, Elizabeth A. Hobson

United States Department of Agriculture Wildlife Services: Staff Publications

Dominance hierarchies can provide many benefits to individuals, such as access to resources or mates, depending on their ranks. In some species, rank can emerge as a product of a group’s history of social interactions. However, it can be difficult to determine whether social history is critical to rank in observation-based studies. Here, we investigated rank dynamics in three captive groups of monk parakeets (Myiopsitta monachus). We used experimental social perturbations to test whether social history shapes rank emergence in these groups. Using targeted removals and reintroductions, we tested whether differently ranked individuals could re-take their ranks in hierarchies after …

Distribution Of The Misfolded Isoform Of The Prion Protein In Peripheral Tissues And Spinal Cord Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Naturally Occurring Chronic Wasting Disease, Terry R. Spraker, Thomas Gidlewski, J. G. Powers, T. A. Nichols, M. A. Wild

United States Department of Agriculture Wildlife Services: Staff Publications

Chronic wasting disease (CWD) is an infectious transmissible spongiform encephalopathy of cervids associated with the presence of a misfolded prion protein (PrPCWD). Progression of PrPCWD distribution has been described using immunohistochemistry and histologic changes in a single section of brain stem at the level of the obex resulting in scores from 0 (early) to 10 (terminal) in elk with naturally occurring CWD. Here we describe the spread and distribution of PrPCWD in peripheral tissues and spinal cord in 16 wild and 17 farmed Rocky Mountain elk (Cervus elaphus nelsoni) with naturally occurring CWD and correlate these findings with obex scores. …

Predator Telemetry Informs Temporal And Spatial Overlap With Stocked Salmonids In Lake Huron, David G. Fielder, Todd A. Hayden, Thomas R. Binder, Brian S. Dorr, Heidi A. Currier

United States Department of Agriculture Wildlife Services: Staff Publications

Double-Crested Cormorants (Phalacrocorax auratus), Walleyes (Sander vitreus), and Lake Trout (Salvelinus namaycush) are migratory predators that undergo extensive movements in Lake Huron. Stocking of juvenile salmonid fish (Oncorhynchus and Salmo sp.) is an important component of fishery management in Lake Huron and assessing the spatial and temporal extent of predator movements is a useful consideration for determining when and where to stock juvenile fish to reduce predation and maximize survival. Previous investigation indicated that some Walleyes migrate to the main basin of Lake Huron in spring from Saginaw Bay. Similarly, telemetry studies of Lake Trout movement in Lake Huron have …

Role Of Social Structure In Establishment Of An Invasive Large Mammal After Translocation, Lindsay M. Clontz, Anni Yang, Sarah M. Chinn, Kim M. Pepin, Kurt C. Vercauterten, George Wittemyer, Ryan S. Miller, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Background

Data on the movement behavior of translocated wild pigs is needed to develop appropriate response strategies for containing and eliminating new source populations following translocation events. We conducted experimental trials to compare the home range establishment and space-use metrics, including the number of days and distance traveled before becoming range residents, for wild pigs translocated with their social group and individually.

Results

We found wild pigs translocated with their social group made less extensive movements away from the release location and established a stable home range ~5 days faster than those translocated individually. We also examined how habitat quality …

Interspecific Oral Rabies Vaccine Bait Competition In The Southeast United States, Wesley C. Dixon, Jacob E. Hill, Richard Chipman, Amy J. Davis, Amy Gilbert, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan

United States Department of Agriculture Wildlife Services: Staff Publications

The United States Department of Agriculture’s National Rabies Management Program (NRMP) has coordinated the use of oral rabies vaccination (ORV) to control the spread of raccoon rabies virus variant west of the Appalachian Mountains since 1997. Working with state and local partners, the NRMP deploys ORV baits containing a rabies vaccine, primarily targeting raccoon populations (Procyon lotor). Bait competition between raccoons and non-target species may limit the effectiveness of ORV programs, but the extent of bait competition remains poorly quantified, particularly in the southeastern United States. We placed placebo ORV baits in bottomland hardwood (n = 637 baits) and upland …

Olfactory Organ Morphology And Ultrastructure Of The Larval Koh Tao Island Caecilian (Ichthyophis Kohtaoensis), Jaclyn M. Patmore

Cal Poly Humboldt theses and projects

The olfactory system of living amphibians (Lissamphibia) undergoes major changes as it transitions from an aquatic to a terrestrial system during metamorphosis. Patterns of change in the cellular morphology of the nose have been examined for frogs (Anura) and salamanders (Caudata). However, it remains unknown if caecilians (Gymnophiona) have similar patterns of change in their nasal ultrastructure. In particular, no data on larval caecilian olfactory cell types are available. Here, using light microscopy and transmission electron microscopy, I examined the olfactory organ of larvae of the caecilian Ichthyophis kohtaoensis, to establish the ultrastructure of the epithelium and compare it …

Blacktip Shark (Carcharhinus Limbatus) Jaw, Laura D. Nicolas, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Jaw from blacktip shark (Carcharhinus limbatus)

Oregon State Rank Assessment For California Slender Salamander (Batrachoseps Attenuatus), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for California Slender Salamander (Batrachoseps attenuatus) using NatureServe methodology, 2023

Relating Predator Community Ecology And Duck Nest Survival In Eastern South Dakota, Samantha R. Fino

Electronic Theses and Dissertations

As conversion of native grasslands and wetlands to croplands continues in the Prairie Pothole Region (PPR), managers are tasked with maintaining sustainable waterfowl populations. Mesopredator community dynamics is a hypothesized mechanism driving spatiotemporal variation of waterfowl nest survival in the PPR, yet studies often lack detailed information on mesopredator species occurrence and abundance. Therefore, understanding spatial and temporal variation in behaviors of mesopredators provides valuable insights for understanding predator-prey interactions between mesopredators and upland duck nests. Further, differences in a predator community resulting from lethal removal of dominant species may influence composition and space use patterns of subordinate and non-target …

The Black Sea-Eastern Mediterranean Flyway Of The Globally Threatened European Turtle Dove (Streptopelia Turtur), Antonio Bea, Iñaki Olano, Saulius Svazas, Julen Henry, Vadym Yanenko, Gennady Grishanov

Turkish Journal of Zoology

The European turtle dove Streptopelia turtur (hereafter turtle dove), is a widespread species in the Palearctic. Due to a large and rapid decline in its abundance, it was classified as "vulnerable" on the International Union for Conservation of Nature's Red List in 2015 and is now regarded as a globally threatened species. The objective of this study was to provide new information on the flyways of this turtle dove population by analyzing available ring recoveries and citizen science sightings data from the Global Biodiversity Information Facility. A new flyway (possibly stretching through the Black and Caspian Seas, Turkey, the Middle …

Key To Isotomidae (Collembola) Of Iran And A Photographic Guide Of Key Characters Of Species, Elham Yoosefi Lafooraki, Jalil Hajizadeh, Masoumeh Shayanmehr, Reza Hosseini, Pietro Paolo Fanciulli

Turkish Journal of Zoology

Present work deals with providing an identification key to 60 Iranian species of the family Isotomidae. The photographic support provided here, both light micrographs and scanning electron microscopy (SEM) pictures, can be used as a practical tool for researchers who are not well acquainted with the morphology of Isotomidae.

Predators In Paradise: Ecotourism And Predator-Prey Dynamics In Monteverde, Costa Rica, Andrew Cremeans

Undergraduate Theses, Professional Papers, and Capstone Artifacts

The use of camera trap surveys is increasingly common to investigate recurring diel activity of mammals. Investigation into the temporal overlap of mammals can provide unique insights into predator-prey dynamics. Understanding this relationship is essential to effectively manage and conserve both species. Extensive research across the tropics has found that daily activities of mammals were shaped by thermoregulation and trophic location in food webs. Although broad scale studies of daily patterns of mammals have enhanced our understanding of these constraints, many study sites included were remote protected areas under strict conservation measures. Such protected areas often had full complements of …

Cutaneous Microbiome Of Red Fox (Vulpes Vulpes) Infected With Sarcoptic Mange (Sarcoptes Scabiei), Jacqueline C. Robidoux

Honors Theses and Capstones

Sarcoptic mange is a parasitic skin disease that affects countless mammals worldwide, including red foxes (Vulpes vulpes). The symptoms, such as hair loss, crusting, and the number of mites, vary between individual foxes. Sarcoptic mange damages the skin barrier, which in turn disrupts the biodiversity of bacteria in the microbiome. It is unknown what the biodiversity of bacteria is at each stage of the disease. This experiment will compare the microbiomes of different samples with and without mange; in hopes to reveal a connection between the different severities of sarcoptic mange and the biodiversity of bacteria and fungi …

Hide And Seek: An Exploration Of Antipredator And Predator Avoidance Mechanisms In Orthopodomyia Signifera In Response To Predation From Toxorhynchites Rutilus, Nathaniel Dahlberg

Master's Theses

There have been many observations of larval Orthopodomyia signifera coexisting with the predator Toxorhynchites rutilus. There are three hypotheses that could explain how Or. signifera resists predation from Tx. rutilus. The first hypothesis states that larvae adapt behavioral changes that limit predation. The second hypothesis states thoracic setae serve as a physical defense that prevents Tx. rutilus from grasping Or. signifera. The third hypothesis states Or. signifera possess a chemical defense indicated by aposematic coloration. To test the first hypothesis larval Or. signifera were exposed to conspecific and heterospecific predation cues and their behavior was observed. Both cues …