Digital Commons Network^™

Preliminary Observations Of Genetic Susceptibility Of Elk (Cervus Elaphus Nelsoni) To Chronic Wasting Disease By Experimental Oral Inoculation, Amir N. Hamir, Thomas Gidlewski, Terry R. Spraker, Janice M. Miller, Lynn Creekmore, Michelle Crocheck, Thomas Cline, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

To compare the genetic susceptibility of elk (Cervus elaphus nelsoni) with various alleles of the PRNP gene, which encodes the normal cellular prion protein, to chronic wasting disease (CWD), eight 8-monthold elk calves of 3 genotypes (2 132MM, 2 132LM, and 4 132LL) were orally dosed with CWD-infected brain material from elk. During postinoculation (PI) month 23, both 132MM elk had lost appetite, developed clinical signs of weight loss and central nervous system (CNS) dysfunction, and were euthanized. Two other elk (both 132LM) developed similar clinical signs of disease and were euthanized during PI month 40. All 4 …

Avian Influenza: Human Pandemic Concerns, Donna K. Carver, Elizabeth A. Krushinskie

Zoonotics and Wildlife Disease: Publications

The likelihood that the next human influenza pandemic virus will emerge from the Asian strain of the H5N1 high pathogenic bird influenza virus that is causing widespread outbreaks in Eurasia remains unknown. (See Glossary for italicized terms.) Because these bird influenza outbreaks remain primarily an animal disease, there is hope that a human pandemic can be prevented. Eradication of the H5N1 high pathogenic bird influenza virus needs to occur at the farm level in the countries where it is currently circulating. Funding of prevention, surveillance, and eradication efforts in the countries where outbreaks are occurring or in at-risk countries will …

Chronic Wasting Disease Of Elk And Deer And Creutzfeldt-Jakob Disease Comparative Analysis Of The Scrapie Prion Protein, Zhiliang Xie, Katherine I. O'Rourke, Zhiqian Dong, Allen L. Jenny, Julie A. Langenberg, Ermais D. Belay, Lawrence B. Schonberger, Robert B. Petersen, Wenquan Zou, Quingzhong Kong, Pierluigi Gambetti, Shu G. Chen

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD), a transmissible prion disease that affects elk and deer, poses new challenges to animal and human health. Although the transmission ofCWDto humans has not been proven, it remains a possibility. If this were to occur, it is important to know whether the “acquired” human prion disease would show a phenotype including the scrapie prion protein (PrP^Sc) features that differ from those associated with human sporadic prion disease. In this study, we have compared the pathological profiles and PrP^Sc characteristics in brains of CWD-affected elk and deer with those in subjects with sporadic Creutzfeldt-Jakob …

Department Of Veterinary And Biomedical Sciences: 2006 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Department of Veterinary and Biomedical Sciences Personnel . 9-16

Faculty .. 9

VBMS Researchers, Postdoctoral and Senior Research Associates . 10

VBMS Adjunct and Courtesy Faculty .... 11

Emeriti Faculty .. 11

VBMS Faculty and Staff by Function and Unit .. 9-16

VBMS Honors, Awards and Recognitions, 2006 17-19

VBMS Committee Assignments, 2006/2007 . 20-22

Faculty Profiles . 23-45

Raul G. Barletta, BS, MS, PhD .. 23

Bruce W. Brodersen, BS, DVM, MS, PhD .... 24

Michael P. Carlson, BS, MS, PhD ... 25

Subash Das, BSV c, MVSc, PhD .... 26

Alan R. Doster, DVM, MS, PhD, ACVP . …

Wildlife Tuberculosis In South African Conservation Areas: Implications And Challenges, A.L. Michel, R.G. Bengis, D.F. Keet, M. Hofmeyr, L.M. De Klerk, P.C. Cross, A.E. Jolles, D. Cooper, I.J. Whyte, P. Buss, J. Godfroid

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis, caused by Mycobacterium bovis, was first diagnosed in African buffalo in South Africa’s Kruger National Park in 1990. Over the past 15 years the disease has spread northwards leaving only the most northern buffalo herds unaffected. Evidence suggests that 10 other small and large mammalian species, including large predators, are spillover hosts. Wildlife tuberculosis has also been diagnosed in several adjacent private game reserves and in the Hluhluwe-iMfolozi Park, the third largest game reserve in South Africa.

The tuberculosis epidemic has a number of implications, for which the full effect of some might only be seen in the long-term. …

Michigan Bovine Tuberculosis Eradication Project: Activities Report And Conference Proceedings 2006, Bridget Kavanagh-Patrick

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (TB) is an infectious disease that is close to being eradicated in the United States, but still poses a significant risk to domestic livestock, wildlife, companion animals and humans throughout the world.

The Michigan Bovine TB Eradication Project involves a multi-agency team of experts from the Michigan Departments of Agriculture (MDA), Natural Resources (DNR) and Community Health (DCH); Michigan State University (MSU) and the US Department of Agriculture (USDA).

Advances In Bovine Tuberculosis Diagnosis And Pathogenesis: What Policy Makers Need To Know, Mitchell V. Palmer, W. Ray Waters

Michigan Bovine Tuberculosis Bibliography and Database

The mainstay of tuberculosis diagnosis in cattle and deer has been the tuberculin skin test. Recent advances have allowed the incorporation of blood based assays to the diagnostic arsenal for both cattle and deer. Use of defined and specific antigens has allowed for improved specificity of cell mediated assays in both cattle and deer and advances in antibody tests for tuberculosis have potential for use in free-ranging and captive cervid populations. Combined use of blood-based assays with skin testing will require further understanding of the effect of skin testing on the accuracy of blood based assays. Models of experimental infection …

Characterizing Salmonella Fecal Shedding Among Racehorses In Louisiana, Anna Marie Chapman

LSU Master's Theses

Salmonella is an important intestinal pathogen in horses capable of infecting populations without demonstrating clinical illness. This study was performed to determine the prevalence of Salmonella fecal shedding among racehorses in Louisiana. Three serial fecal samples were collected from 429 Thoroughbred horses housed at four racetracks. Feces were tested for Salmonella by microbiologic culture with selective primary enrichment and delayed secondary enrichment (DSE). Samples were also evaluated for the presence of Salmonella by polymerase chain reaction (PCR) using genus-specific oligonucleotide primers. A total of 7 (1.6%) horses were positive for Salmonella by either primary bacterial culture or DSE and an …

Progress In Diagnosis, Treatment And Elimination Of Echinococcosis And Cysticercosis, Peter M. Schantz

Zoonotics and Wildlife Disease: Publications

Here I review the current status of geographical occurrence and public health significance of echinococcosis (Echinococcus spp. infections) and cysticercosis (Taenia solium infection) with special emphasis on the remarkable technologic progress achieved in recent decades that has led to greater understanding of the biology and epidemiology of these cestode infections. The greatest remaining challenges are to apply this knowledge and technology to improved medical management and prevention of these infections.

Premature Induction Of An Immunosuppressive Regulatory T Cell Response During Acute Simian Immunodeficiency Virus Infection, Jacob D. Estes, Qingsheng Li, Matthew R. Reynolds, Stephen W. Wietgrefe, Lijie Duan, Timothy Schacker, Louis J. Picker, David I. Watkins, Jeffrey D. Lifson, Cavan Reilly, John V. Carlis, Ashley T. Haase

Qingsheng Li Publications

Here we report the results of an investigation into the possibility that one mechanism responsible for the establishment of persistent human immunodeficiency virus infection is an early regulatory T (T_reg) cell response that blunts virus- specific responses. Using the simian immunodeficiency virus (SIV)–infected rhesus macaque model, we show that, indeed, viral replication and immune activation in lymphatic tissue drive a premature immunosuppressive response, with dramatic increases in the frequencies of CD4⁺CD25⁺FOXP3⁺ Treg cells, transforming growth factor–β1⁺ cells, interleukin–10+ cells, and indoleamine 2,3-dioxygenase+CD3⁺ cells.When we compared SIV infection with rhesus cytomegalovirus (RhCMV) …

The Epidemiology Of West Nile Virus In Louisiana, Karen Ruth Gruszynski

LSU Doctoral Dissertations

West Nile virus (WNV), a member of the genus Flavivirus transmitted by mosquitoes, first appeared in the New York in 1999. Within five years WNV was detected throughout the contiguous 48 states causing disease in reservoirs and accidental hosts alike. In Louisiana, WNV was first detected in 2001 with one human case, ten equine cases, and six dead birds reported. The introduction of WNV into Louisiana presented an unique opportunity to observe an emerging disease unfold, so a study was launched to gain insight into the epidemiology of WNV in Louisiana. The first component, an environmental predictive model for West …

Power Analysis For A Mixed Effects Logistic Regression Model, Yinmei Li

LSU Doctoral Dissertations

In herd health studies, the mixed effects logistic regression model with random herd effects are commonly used for modeling clustered binary data. These models are well developed and widely used in the literature, among which is the logistic-normal regression model. In contrast to the rich literature in modeling methods, the sample size/power analysis methods for such mixed effects models are sparse. The sample size/power analysis method for the logistic-normal regression model is not readily available. This study is to develop a power analysis/sample size estimation method for the logistic-normal regression model. Extended from the sample size method for the likelihood …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1- Anthrax, High Risk Pathogen Biocontainment and the ADRDL [Page] 1-Anthrax in South Dakota, Summer 2005 [Page] 3- Transmission of Equine Influenza Virus to Dogs [Page] 5- Reminder Avoid Contaminated Milk Samples [Page] 6- Selenium Toxicosis in Horses and Cattle: Sampling, Diagnosis and Clinical Signs [Page] 7- Center for Infectious Disease Research & Vaccinology Holds Conference in Enteric Diseases [Page] 7- Holiday Hours [Page] 8- Calendar of Events

Examining The Risk Of Disease Transmission Between Wild Dall’S Sheep And Mountain Goats, And Introduced Domestic Sheep, Goats, And Llamas In The Northwest Territories, Elena Garde, Susan Kutz, Helen Schwantje, Alasdair Veitch, Emily Jenkins, Brett Elkin

Zoonotics and Wildlife Disease: Publications

This risk assessment has been carried out following the guidelines for Health Risk Analysis entitled “Wild Animal Translocations” prepared by the Canadian Cooperative Wildlife Health Centre (http://wildlife1.usask.ca). It includes 9 comprehensive appendices of bacterial, viral, parasitic and fungal organisms reported from domestic sheep, goats, llamas and wild sheep and mountain goats. The report is a breakdown of those appendices into discussions of organisms of major concern, organisms of unknown concern, organisms of minimal concern, and those that cause no apparent disease, are not transmissible between the species of interest, or do not occur in Canada. Where possible, organisms were assigned …

United States Patent: D-Alanine Racemase Mutants Of Mycobacteria And Uses Therefore, Raul G. Barletta, Ofelia Barletta-Chacon

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention is directed to D-alanine racemase mutants of mycobacterial species. The D-alanine racemase gene (alrA) is involved in the systhesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the bacterial cell wall. The present invention is also directed to methods of making live-attenuated vaccines against pathogenic mycobacteria using such alrA mutants and to the vaccines made according to such methods. The present invention is further directed to use of alrA mutants in methods for screening antimycobacterial agents that are synergistic with peptidoglycan inhibitors. Finally, the present invention is directed to methods to identify new …

Sheep Updates 2005 - Contents, Department Of Agriculture Western Australia

Sheep Updates

No abstract provided.

Sheep Updates 2005 - Part 3, Rob Davidson, David Pethick, C. F. Engelke, B. D. Siebert, K. Gregg, A-D G. Wright, P. E. Vercoe, Robin Jacob, Russell Barnett, Joanne Sneddon, Sandra Brown, G. Rose, C. Kabore, J Dart, Stuart Adams

Sheep Updates

This session covers seven papers from different authors: CUSTOMER 1. Benefits VIAscanR to producers and WAMMCO, Rob Davidson, Supply Development Manager, David Pethick, School of Veterinary and Biomedical Studies, Murdock University. 2. Healthy fats in lamb: how WA lambs compare with others, C. F. Engelke Animal Biology, University of Western Australia, bCSIRO Livestock Industries, Western Australia B.D. Siebert, Department of Animal Science, University of Adelaide, South Australia, K. Gregg, Centre for High-Throughput Agricultural Genetic Analysis, Murdoch University, Western Australia. A-D.G. Wright CSIRO Livestock Industries, Western Australia, P.E Vercoe Animal Biology, University of Western Australia 3. Shelf life of fresh lamb …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message Diagnostic News [Page] 1- New reusable mailers for biopsy submission [Page] 1-New mastitis submission form, procedures [Page] 2- Trichomonas testing at SDSU ADRDL adheres to OEI standards [Page] 2- Announcement [Page] 2- Holiday Hours [Page] 3- BVD diagnostics at SDSU ADRDL: Demonstrating persistently infected cattle via antigen detection Extension News [Page] 6- NCVEI looking for help from large animal veterinarians [Page] 7- New dean of agriculture on the job at SDSU [Page] 7- Thaler named interim Animal & Range [Page] 8- Calendar of Events

Animal Health Laboratories Service Manual 2005-2006, Department Of Agriculture And Food, Western Australia

Agriculture reports

This service manual is designed to help you, the user of services provided by the Animal Health Laboratories (AHL), to get the most from the laboratories.

The section on ‘General Instructions’ contains information required for packaging and submission of samples to AHL.

Other sections contain information on the type of sample required for each laboratory service and associated tests offered by AHL. The detail of Services offered by each laboratory is given in a table, followed by collection notes to assist with submission of specimens.

Each table describes the Services offered, the Tests available for each Service, a description of …

Antibody Responses In Reindeer (Rangifer Tarandus) Infected With Mycobacterium Bovis, W. R. Waters, M. V. Palmer, J. P. Bannantine, R. Greenwald, J. Esfandiari, P. Andersen, J. Mcnair, J. M. Pollock, K. P. Lyashchenko

Michigan Bovine Tuberculosis Bibliography and Database

Despite having a very low incidence of disease, reindeer (Rangifer tarandus) are subject to tuberculosis (TB) testing requirements for interstate shipment and herd accreditation in the United States. Improved TB tests are desperately needed, as many reindeer are falsely classified as reactors by current testing procedures. Sera collected sequentially from 11 (experimentally) Mycobacterium bovis-infected reindeer and 4 noninfected reindeer were evaluated by enzyme-linked immunosorbent assay (ELISA), immunoblotting, and multiantigen print immunoassay (MAPIA) for antibody specific to M. bovis antigens. Specific antibody was detected as early as 4 weeks after challenge with M. bovis. By MAPIA, sera …

Weast Nile Virus Serosurveillance In Iowa White-Tailed Deer (1999-2003), Julian Santatella, Robert Mclean, Jeffrey S. Hall, James S. Gill, Richard A. Bowen, Harlo H. Hadow, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

Sera from white-tailed deer (Odocoileus virginianus) were collected in Iowa during the winter months (1999-2003), 2 years before and after West Nile virus (WNV) was first reported in Iowa (2001), and were analyzed for antibodies to WNV. Samples from 1999 to 2001 were antibody negalive by blocking enzyme-linikedI immunosorbent assay (bELISA) and plaque reduction neutralization test (PRNT₉₀). Prevalence derived from bELISA (2002, 12.7%; 2003. 11.2%) and WNV PRNT₉₀ (2002,7.9%; 2003, 8.5%) assays were similar. All sanlples were negative for antibodies against St. Louis encephalitis virus as determined by PRNT₉₀. Antibodies to flaviviruses were …

Characterization Of Cytokine Induction And Effects Of Antiviral Treatment In Four Murine Models Of Poxvirus Infection, Corinna W. Knorr

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cytokine profiles during cowpox virus (CPV) strain Brighton and vaccinia virus (VV) strain Western Reserve infections were characterized in intranasal (i.n.) and intraperitoneal (i .p.) models in BALB/c mice. The time-course of induction and effects of cidofovir treatment on interferon (IFN)-γ, IFN-γ inducible protein (IP)-10, interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1 were determined. The four models have distinct patterns of cytokine induction. CPV i.p. and VV i.n. infections showed increased induction throughout the time studied. CPV i.n. infection resulted in delayed induction of IFN-γ and IP-10. Cytokine levels were fairly constant during VV i.p. infections. Cidofovir treatment (100 mg/kg/day …

Tularemia: Emergence/Re-Emergence, Jeannine M. Petersen, Martin E. Schriefer

Zoonotics and Wildlife Disease: Publications

Francisella tularensis is a gram-negative coccobacillus and the etiologic agent of the zoonotic disease tularemia. First described in 1911 in Tulare County, California, it has since been reported throughout the Northern Hemisphere, with natural infections reported among an unusually wide range of vertebrates and invertebrates. In recent years, tularemia has emerged in new geographic locations, populations, and settings. This review will serve to highlight mechanisms contributing to the recent emergence of tularemia as well as a repertoire of diagnostic tools useful for detecting and diagnosing disease.

5-Ht1b Receptor-Mediated Presynaptic Inhibition Of Gaba Release In The Suprachiasmatic Nucleus, Jayne R. Bramley, Patricia J. Sollars, Gary E. Pickard, F. Edward Dudek

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) receives a dense serotonergic innervation that modulates photic input to the SCN via serotonin 1B (5-HT_1B) presynaptic receptors on retinal glutamatergic terminals. However, the majority of 5-HT_1B binding sites in the SCN are located on nonretinal terminals and most axonal terminals in the SCN are GABAergic. We therefore tested the hypothesis that 5-HT_1B receptors might also be located on SCN GABAergic terminals by examining the effects of the highly selective 5-HT_1B receptor agonist CP-93,129 on SCN miniature inhibitory postsynaptic currents (mIPSCs). Whole cell patch-clamp recordings of mIPSCs were obtained from rat …

Chronic Wasting Disease (Cwd) Data Clearinghouse

Zoonotics and Wildlife Disease: Publications

The National Biological Information Infrastructure (NBII) is an electronic information network that provides access to biological data and information on our nation’s plants, animals, and ecosystems. Information contributed by federal, state, and local government agencies; non-government organizations; and private-sector organizations is linked through the NBII gateway and made accessible to a variety of audiences, including researchers, natural resource managers, decision-makers, educators, students, and other members of the general public. Implementation of the NBII is being accomplished through the development of "nodes" that serve as entry points to the network. These nodes function as fully digital, distributed, and interactive systems that …

Evaluation Of Western Blotting Methods Using Samples With Or Without Sodium Phosphotungstic Acid Precipitation For Diagnosis Of Scrapie And Chronic Wasting Disease, Hongsheng Huang, Jasmine Rendulich, Dan Stevenson, Katherine I. O'Rourke, Aru Balachandran

Zoonotics and Wildlife Disease: Publications

The purpose of this study was to enhance the sensitivity of the Western blot (WB) test for use as an alternative and confirmatory method for the diagnosis of scrapie and chronic wasting disease (CWD) in Canada by comparing 2 sample preparation procedures: an abnormal prion protein (PrP^Sc) concentration procedure using sodium phosphotungstic acid (PTA) precipitation and a procedure using crude sample without precipitation. A total of 100 cerebrum samples (52 sheep and 48 elk), including 66 negative (31 sheep, 35 elk) and 34 positive (21 scrapie and 13 CWD positive) samples diagnosed by using immunohistochemistry (IHC) on retropharyngeal …

The Incidence Of Genotypes At Codon 171 Of The Prion Protein Gene (Prnp) In Five Breeds Of Sheep And Production Traits Of Ewes Associated With Those Genotypes, B. M. Alexander, R. H. Stobart, W. C. Russell, Katherine I. O'Rourke, G. S. Lewis, J. R. Logan, J. V. Duncan, G. E. Moss

Zoonotics and Wildlife Disease: Publications

Scrapie is one of several transmissible spongiform encephalopathies of livestock. Disease susceptibility is linked to polymorphisms in the normal prion protein gene that encodes the mammalian prion precursor. Codon 171 of this gene is a major determinant of scrapie susceptibility. Selection for arginine (R) at codon 171 is encouraged by the USDA to decrease the incidence of scrapie. Objectives of this study were to determine the frequency of R allele variants at codon 171 in a sample of sheep from five breeds (Columbia, Hampshire, Rambouillet, Suffolk, and Targhee) and western white-faced commercial ewes and to determine whether the R allele …

Chronic Wasting Disease Of Elk: Transmissibility To Humans Examined By Transgenic Mouse Models, Qingzhong Kong, Shenghai Huang, Wenquan Zou, Difernando Vanegas, Meiling Wang, Di Wu, Jue Yuan, Mengjie Zheng, Hua Bai, Huayun Deng, Ken Chen, Allen L. Jenny, Katherine I. O'Rourke, Ermais D. Belay, Lawrence B. Schonberger, Robert B. Pertersen, Man-Sun Sy, Shu G. Chen, Pierluigi Gambetti

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD), a prion disease affecting free-ranging and captive cervids (deer and elk), is widespread in the United States and parts of Canada. The large cervid population, the popularity of venison consumption, and the apparent spread of the CWD epidemic are likely resulting in increased human exposure to CWD in the United States. Whether CWDis transmissible to humans, as has been shown for bovine spongiform encephalopathy (the prion disease of cattle), is unknown. We generated transgenic mice expressing the elk or human prion protein (PrP) in a PrP-null background. After intracerebral inoculation with elk CWD prion, two lines …

Predictive Spatial Dynamics And Strategic Planning For Raccoon Rabies Emergence In Ohio, James E. Childs, David L. Smith, Colin A. Russell, Leslie A. Real

Zoonotics and Wildlife Disease: Publications

Rabies is an important public health concern in North America because of recent epidemics of a rabies virus variant associated with raccoons. The costs associated with surveillance, diagnostic testing, and post-exposure treatment of humans exposed to rabies have fostered coordinated efforts to control rabies spread by distributing an oral rabies vaccine to wild raccoons. Authorities have tried to contain westward expansion of the epidemic front of raccoonassociated rabies via a vaccine corridor established in counties of eastern Ohio, western Pennsylvania, and West Virginia. Although sporadic cases of rabies have been identified in Ohio since oral rabies vaccine distribution in 1998, …

Experimental Transmission Of Chronic Wasting Disease Agent From Mule Deer To Cattle By The Intracerebral Route, Amir N. Hamir, Robert A. Kunkle, Randall C. Cutlip, Janice M. Miller, Katherine I. O'Rourke, Elizabeth S. Williams, Michael W. Miller, Mick J. Stack, Melanie J. Chaplin, Jügen A. Richt

Zoonotics and Wildlife Disease: Publications

This communication reports final observations on experimental transmission of chronic wasting disease (CWD) from mule deer to cattle by the intracerebral route. Thirteen calves were inoculated intracerebrally with brain suspension from mule deer naturally affected with CWD. Three other calves were kept as uninoculated controls. The experiment was terminated 6 years after inoculation. During that time, abnormal prion protein (PrP^res) was demonstrated in the central nervous system (CNS) of 5 cattle by both immunohistochemistry and Western blot. However, microscopic lesions suggestive of spongiform encephalopathy (SE) in the brains of these PrP^res-positive animals were subtle in 3 …