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Sulfated Flavonoids In The Asteraceae, Alyssa Rice

Senior Honors Projects

Gypsum soils are a unique soil type with high levels of calcium and sulfur, which creates a harsh living environment for plants. To survive these conditions, plants have evolved a suite of mechanisms to survive these excess minerals, such as sulfur. In this study we aimed to determine if gypsum status was related to sulfated flavonoid production in plants of the Asteraceae. Flower and leaf tissues were collected from herbarium specimens representing gypsophiles, gypsovags, and gypsofuges. These tissues were analyzed for the presence of sulfated flavonoids using Thin Layer Chromatography (TLC). We observed sulfated flavonoid production in leaf and flower …

Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

DNA methylation is an important epigenetic modification required for the specific regulation of gene expression and the maintenance of genome stability in plants and animals. However, the mechanism of DNA demethylation remains largely unknown. Here, we show that two SGS3-like proteins, FACTOR OF DNA DEMETHYLATION 1 (FDDM1) and FDDM2, negatively affect the DNA methylation levels at ROS1-dependend DNA loci in Arabidopsis. FDDM1 binds dsRNAs with 50 overhangs through its XS (rice gene X and SGS3) domain and forms a heterodimer with FDDM2 through its XH (rice gene X Homology) domain. A lack of FDDM1 or FDDM2 increased DNA methylation levels …

Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood Ii, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable

Center for Plant Science Innovation: Faculty Publications

Motivation: Over the last decade, RNA-Seq whole-genome sequencing has become a widely used method for measuring and understanding transcriptome-level changes in gene expression. Since RNA-Seq is relatively inexpensive, it can be used on multiple genomes to evaluate gene expression across many different conditions, tissues and cell types. Although many tools exist to map and compare RNA-Seq at the genomics level, few web-based tools are dedicated to making data generated for individual genomic analysis accessible and reusable at a gene-level scale for comparative analysis between genes, across different genomes and meta-analyses. Results: To address this challenge, we revamped the comparative gene …

Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson, Fiona Goggin

Inquiry: The University of Arkansas Undergraduate Research Journal

Plant elicitor peptides (Peps) – endogenous chains of amino acids involved in natural plant defense – have been shown to decrease damage from herbivores and pathogens by inducing an immune response, increasing the emission of volatile organic compounds (VOCS), transcripts, and metabolites. Exogenous treatment of soybean seeds with plant elicitor peptide GmPep3 has been shown to induce these broad-spectrum defenses and offers a new method for increasing crop yield. However, the effects of GmPep3 on indicators of soybean health – root characteristics, growth stages, etc. – have not been fully realized. Using the root-phenotyping platform RhizoVision Explorer, several root traits …

A Phytobacterial Tir Domain Effector Manipulates Nad+ To Promote Virulence, Samuel Eastman, Thomas Smith, Mark A. Zaydman, Panya Kim, Samuel Martinez, Neha Damaraju, Aaron Diantonio, Jeffrey Milbrandt, Thomas Clemente, James R. Alfano, Ming Guo

Department of Agronomy and Horticulture: Faculty Publications

The Pseudomonas syringae DC3000 type III effector HopAM1 suppresses plant immunity and contains a Toll/interleukin-1 receptor (TIR) domain homologous to immunity-related TIR domains of plant nucleotide-binding leucine-rich repeat receptors that hydrolyze nicotinamide adenine dinucleotide (NAD+) and activate immunity. In vitro and in vivo assays were conducted to determine if HopAM1 hydrolyzes NAD+ and if the activity is essential for HopAM1’s suppression of plant immunity and contribution to virulence. HPLC and LC-MS were utilized to analyze metabolites produced from NAD+ by HopAM1 in vitro and in both yeast and plants. Agrobacterium-mediated transient expression and in planta inoculation assays were performed to …

Geospatial Data Trees 1981, Grace K. Attea, Ryan W. Mcewan, John L. Vankat

Data Files for Old-Growth Deciduous Forest Dynamics Archive: Hueston Woods

Location and demographic data collected for each individual tree in 1981 in Hueston Woods State Nature Preserve. These maps were digitized from the historical field maps to a GIS shapefile for each sampling year. Geographic Coordinate System: GCS_North_American_1983. Projected Coordinate System: NAD_1983_StatePlane_Ohio_South_FIPS_3402 (meters).

Dr. Laurie L. Consaul Herbarium (Uwo), Lauren H. Rego

Community Engaged Learning Final Projects

Along with the provided databased species, students scanned 257 specimens within the physical herbarium at Wester.

The Quest To Identify A New Virus Disease Of Sunflower From Nebraska, Robert M. Harveson, Maher Al Rwahnih, California Department Of Food And Agriculture, Alex Karasev, Tom J. Gulya, Jeff Bradshaw

Panhandle Research and Extension Center

Between 2010 and 2018, sunflower plants exhibiting virus-like symptoms, including stunting, mottling, and chlorotic ringspots on leaves, were observed from commercial fields and research plots from four sites within three distinct counties of western Nebraska (Box Butte, Kimball, and Scotts Bluff). Near identical symptoms from field samples were reproduced on seedlings mechanically in the greenhouse on multiple occasions, confirming the presence of a sap-transmissible virus from each site. Symptomatic greenhouse-inoculated plants from the 2010 and 2011 Box Butte samples tested negative for sunflower mosaic virus (SuMV), sunflower chlorotic mottle virus (SuCMoV), and all potyviruses in general by ELISA and RT-PCR. …

Bioprospecting For Improved Floral Fragrance In Wild Sunflower, Jason Anandappa

Honors Undergraduate Theses

The cultivation of crop sunflower or Helianthus annuus is a 20 billion USD industry globally. Crop wild relatives of H. annuus have a long history of being used to breed improved traits into cultivated varieties. Cultivated H. annuus is not known to have a pleasant aroma, and at times seed yield is limited by pollination services, particularly in the production of hybrid seed. Improved floral fragrance could improve pollinator attraction and would add value to ornamental sunflowers in the context of the cut-flower industry. If volatile organic compounds that together generate favorable scents are present in wild Helianthus species, they …

Investigating The Phytochemical Profiles Of Wild And Cultivated Blueberries (Vaccinium Spp.) In Central Florida, Anisa Khalid

Honors Undergraduate Theses

Blueberries (Vaccinium spp.) are valued for their high nutritional quality and flavor. There are five species in the Vaccinium genus native to the state of Florida, but only three are palatable to humans: V. myrsinites, V. darrowii, and V. corymbosum. While V. darrowii has been studied extensively due to its successful hybridization with northern highbush blueberry cultivars, V. myrsinites and wild V. corymbosum have been understudied. This study aims to 1) quantify the abundance of various phytochemicals in fruits of these wild blueberry species and compare fruit quality both among the wild taxa and two varieties of …

Proceedings Of The 2022 Passion Fruit Conference: Growing The U.S. Passion Fruit Industry A Strategic Conference For: Growers, Marketers, Researchers, And Stakeholders, Eric T. Stafne

CREC Publications

We are pleased to deliver these proceedings of the passion fruit conference that was held in Homestead, Florida on June 14 and 15, 2022. It was a culmination of a lot of hard work by many people, most of them listed in the previous page. However, it would not have been a success without the attendees who came from various parts of the country to share their experiences growing passion fruit. What follows in these proceedings are the slides of the presentations with by notes taken by attendees along with notes taken of discussion regarding the five priority topic areas: …

Intercropping Grain Sorghum Into Established Rhizoma Peanut: Greenhouse And Field Studies, Erika Hm Cooperman

Graduate Theses/Dissertations

Land degradation and urbanization are among the top factors pushing the Kenyan Maasai community into an unstable future, with food insecurity and poverty amidst the most fragile components. Implementing conservation agricultural techniques into the Maasai nomadic lifestyle could potentially lead to a diversification of finances and food security. Intercropping is one technique of conservation agriculture that could provide the Maasai both. The main objective of this study is to evaluate the effects of creating an intercropped environment between rhizoma peanut and grain sorghum. A greenhouse study was conducted from late 2020 to 2021 in an effort to investigate the effects …

Testing The Impact Of A Silencing Suppressor On Infectivity Of Grapevine Vein Clearing Virus Infectious Clone In Nicotiana Benthamiana, Wen Zhao

Graduate Theses/Dissertations

Grapevine vein clearing virus (GVCV), the first DNA virus of the Badnavirus genus discovered in grapevines, is closely associated with grapevine vein-clearing disease. Through earlier research, Koch's postulates were partially met: GVCV was in all diseased plants; GVCV was introduced into a healthy grapevine through grafting and by aphids and caused the disease. However, more shreds of evidence are required to fulfill the last postulate, the same virus must be reisolated from the inoculated diseased grapevine (1). A full-length infectious clone of GVCV was previously constructed to provide evidence; however, its infectivity was not consistent. Therefore, the goal …

Forage News [2022-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

Review Of Spiders Of North America, By Sarah Rose, Michael L. Draney

The Prairie Naturalist

A field guide to spiders, the dominant terrestrial predator group, has long been a desire for North American naturalists, biologists, and spider fans, but not surprisingly, the first “real” field guide has been a long time coming. The diversity of spiders, with over 50,000 known species globally (World Spider Catalog 2022) and around 4,000 in North America north of Mexico (Rose 2022), is well beyond the diversity of more traditional field guide targets such as birds (about 1,000 species north of Mexico; American Birding Association 2022) or butterflies (about 725 species north of Mexico; North American Butterfly Association 2022). Also, …

Review Of Methods For Ecological Research On Terrestrial Small Mammals, By Robert Mccleery, Lynda R. Lafond

The Prairie Naturalist

Small mammals are used in ecological studies throughout the world, yet the subject has lacked a clear reference that included upto- date methods pertaining to passive detection, molecular, and statistical approaches. In Methods for Ecological Research on Terrestrial Small Mammals, McCleery and his coauthors build on previous research references (Braun 2005, Sutherland 2006, Krebs 2014, Silvy 2020) and present methodologies specific to the study of small mammals that are applicable globally. Written as an asset for novice and experienced small mammal researchers alike, Methods for Ecological Research on Terrestrial Small Mammals is organized in a logical format, providing readers …

Second Twenty-Five Year Index: Volumes 26–50, 1994–2018, Jane E. Austin

The Prairie Naturalist

The first 50 years of the Prairie Naturalist reside on the shelves of libraries and personal collections. The articles and notes published over those years provide invaluable knowledge about Great Plains biota, communities, conservation, and landscape ecologies. Awareness of the wealth of information in those 50 print volumes has been limited, relying on references from other published papers or the limited number of articles that have been made available online by authors. This index serves as resource for individuals to digitally tap into the information published since 1994. This index, together with the first 25-year index, serves to complement the …

West Virginia Herbaria: Status, Updates, And Plans, Pamela Puppo, Katharine Gregg, Donna Ford-Werntz, James Vanderhorst, Michelle Mabry, Jeremy Keene

Biological Sciences Faculty Research

The West Virginia (U.S.A.) Herbarium Curators met on December 1st, 2021, with the intent of sharing updates on the collections, fostering collaboration, learning from each other's experiences, and identifying priorities for the collections towards the future. This article presents a summary of the herbaria that were represented in this meeting.

Predicting Munsell Color For Turfgrass Leaves, William L. Berndt, Roch E. Gaussoin

Department of Agronomy and Horticulture: Faculty Publications

Linking turfgrass color to hue, value, chroma (H V/C) in the Munsell Plant Tissue Color Book is a visual comparison process for specifying and communicating plant color. If subjectivity of visual comparison can be mitigated, then accuracy of color matching may be improved. Research was conducted to develop an algorithm predicting H V/C from CIE-xyY color (xyY) in digital images of leaves of four turfgrasses. First, value-chroma (V/C) arrays for Munsell hue groups 5Y, 2.5GY, 5GY, 7.5GY, 10GY, and 2.5G were converted to xyY. Next, chromaticity (xy) plots from each array were fitted with …

Regenerating Agroecosystems By Overcoming Human Exceptionalism In Designing For Increased Equity Of Benefits From Ecoservices, Ali Loker, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Our commentary explores three critical issues related to ecosystem services. First is how ecoservices are currently designed and implemented primarily for human benefit without concern for how these impact other species. We conclude that awareness of this imbalance is the first step toward meaningful change. Second we observe that human exceptionalism guides most decisions, and ask whether we can overcome this mind-set to embrace ecoregeneration and design of resilient and mutually beneficial agroecosystems. Our attitude toward the challenge and moving toward greater humility about human roles that guide management decisions in the ecosystem is a requisite for change. Third we …

Tallgrass Prairie Center 2021-2022 Highlights, University Of Northern Iowa. Tallgrass Prairie Center.

Annual Reports

Contents:

--- Staff
--- From Our Director
--- Iowa Roadside Management: Enhancing our public roadsides
--- Research and Restoration: Improving prairie plantings in agricultural landscapes
--- Plant Materials: Iowa-source genetic diversity for Iowa’s roadsides and beyond
--- Prairie on Farms: Making prairie practical
--- Irvine Prairie: Restoring a piece of the original Iowa landscape
--- Green Iowa AmericCorps: Land and Water Stewards expand TPC capacity
--- Prairie Roots Project: A deeper appreciation of the prairie ecosystem
--- TPC Publications and Outreach
--- Friends of the TPC: Supporting the TPC's mission
--- The Prairie in Art: Erin Anfinson
--- Iowa STEM …

Modeling Jadera Haematoloma’S Phenotypic Variation In The Context Of Its Developmental Plasticity, Michael C. Yorsz

Honors Theses

Phenotypic plasticity is the ability of an organism to integrate information from environmental cues to inform the development of its phenotype and remains understudied in biology. Models of plasticity are needed because evolution in the presence of plasticity is poorly understood. Jadera haematoloma, a hemimetabolous true bug, is an excellent animal model of plasticity, exhibiting a non-linear plastic response to juvenile nutrition that biases adult development into groups with differences in flight capability, wing shape, and fecundity. However, there is a lack of literature consensus regarding the range of developmental outcomes in the species. Some publications report the presence …

Sherwin Carlquist (1930–2021)—A Botanical Luminary, Thomas S. Elias, Mare Nazaire, Gary D. Wallace, Vanessa E. Ashworth

Aliso: A Journal of Systematic and Floristic Botany

Sherwin Carlquist (1930–2021) was an internationally respected and distinguished botanist who held faculty positions in botany at California Botanic Garden, Claremont Graduate University and Pomona College between 1956 and 1992. His legacy includes major scholarly contributions to plant systematics, plant anatomy, especially wood anatomy, island biogeography, evolutionary and ecological reasoning, and a prolific publication record. A loose collection of paragraphs by those who interacted with him addresses Carlquist's tremendous botanical output, teaching, mentorship, scientific scholarship, and his roles as a colleague and friend.

Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels

Electronic Theses and Dissertations

Conventional agricultural practices can have unintended consequences on the environment and wildlife. Insects and birds are declining at rapid rates around the world, and the current conventional agricultural paradigm is a major driver through habitat loss and the intensification of production. Invertebrates in agroecosystems provide services to both farmers and the rest of society. Regenerative systems may promote the functioning of an agroecosystem by influencing invertebrate abundance, diversity, and ecosystem services and mitigate bird and insect declines through conservation practices that increase soil health, reduce disturbances, and increase biological diversity. Here I address knowledge gaps of the effects of regenerative …

Response Of Soil Biochemical And Physical Properties To Long Term Prairie Cordgrass And Kura Clover Intercropping System, Vaishnavi Varikuti

Electronic Theses and Dissertations

Prairie cordgrass (Spartina pectinata) (PCG) is a warm-season perennial grass that can be used as a biofuel feedstock and can be grown on marginal lands. Previous studies on intercropping of a perennial legume i.e., kura clover (Trifolium ambiguum) (KC) with PCG can improve soil biochemical properties, increase biomass production, mitigate greenhouse gas (GHG) emissions while reducing the chemical fertilizer requirement. However, there is a lack of evidence about the effects of PCG production on soil biochemical and physical properties during the cropping season and at deeper soil depths in reference to support plant growth, environmental implications and enhance the soil …

Machine-Learning And Meta-Analysis Techniques To Quantify And Predict Soil Organic Carbon, N2O-N And Co2-C Emissions In Cover Crop Systems, Deepak Raj Joshi

Electronic Theses and Dissertations

People worldwide are challenged by multiple threats including climate change, growing populations, and soil degradation. Addressing these challenges requires understanding of the local environment, farming systems and modern technologies. These technologies include new ways to process information that include artificial intelligence, machine learning and meta-analysis. Models produced using these technologies may be useful for predicting the consequences of implementing conservation practices that reduce GHG emissions as well as for determining the carbon footprint of cropping systems that include environmentally friendly conservation technologies such as growing cover crop. Therefore, our objectives of this study were to: 1) provide an overview of …

Effects Of Buried Wood On The Development Of Populus Tremuloides On Various Oil Sands Reclamation Soils, Kaitlyn E. Trepanier, Laura Manchola-Rojas, Bradley D. Pinno

Aspen Bibliography

Buried wood is an important but understudied component of reclamation soils. We examined the impacts of buried wood amounts and species on the growth of the common reclamation tree species trembling aspen (Populus tremuloides). In a greenhouse study, aspen seedlings were planted into four soil types, upland derived fine forest floor-mineral mix (fFFMM), coarse forest floor-mineral mix (cFFMM), and lowland derived peat and peat-mineral mix (PMM), that were mixed with either aspen or pine wood shavings at four concentrations (0%, 10%, 20% and 50% of total volume). Height and diameter growth, chlorophyll concentration, and leaf and stem biomass were measured. …

All About The Prairie: Coloring Book Of Plants, Animals, And Insects Native To Iowa Prairies, Brianna Hull

Faculty Book Gallery

A coloring book with drawings of plants, animals, and insects that are native to Iowa. Includes the common and Latin names of the species as well as the Meskwaki names for some. -- Provided by the publisher

Current And Past Prairie Reconstruction Approaches, Sophie Wieland

Honors Capstone Projects

This project was concerned with prairie reconstruction - that is, lands that have been seeded and are being managed to emulate a prairie ecosystem. The first section is a literature review on how prairie reconstruction arose. In the second component, data on plant species in local reconstructed prairies collected for the Prairie Reconstruction Initiative by the Morris Wetland Management District were summarized. The new monitoring framework is evaluated, as is the condition of the selected reconstructions, including the success of planted species and encroachment of non-native species.

Phenotypic Strategies For Jasione L. Or The Challenge That Taxonomy Face To Recent Floristic Responses Along Western Eurasia, João Rocha, Paulo Almeida, Rubén Ramírez-Rodríguez, Fátima Sales, Francisco Amich, Antonio Crespi

Turkish Journal of Botany

Jasione L. (Campanulaceae) is regarded as one of the recent floristic responses in western Eurasia. The characterization of the morpho-environmental fluctuating asymmetries (FA) to describe the phenotypic responses for Jasione L. is here exposed. Continuity is pointed out to describe these morpho-environmental responses, as a redundancy effect (RE), in accordance with the significant similarity involved. In this case, 327 individuals and 14 species were investigated (J. sphaerochephala Brullo, Marcenò & Pavone and J. idaea Stoj. synonymized as J. montana L. var. montana and J. supina Sieber ex Spreng. subsp. pontica (Boiss.) Damboldt). J. montana L. and J. heldreichii Boiss. & …