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Phenology And Resource Allocation Strategies Of Diploid Flowering Rush (Butomus Umbellatus) In Ohio And New York, Maxwell G. Gebhart, Ryan M. Wersal, Andrew R. Hannes, Nathan E. Harms, Bradley T. Sartain, William L. Wolanske, Mia Yeager

Biological Sciences Department Publications

Flowering rush (Butomus umbellatus L.) is an emergent perennial monocot that has invaded aquatic systems along the U.S.–Canadian border. Currently, there are two known cytotypes of flowering rush, diploid and triploid, within the invaded range. Although most studies have focused on the triploid cytotype, little information is known about diploid plants. Therefore, phenology and resource allocation were studied on the diploid cytotype of flowering rush in three study sites (Mentor Marsh, OH; Tonawanda Wildlife Management Area, NY; and Unity Island, NY) to understand seasonal resource allocation and environmental influences on growth, and to optimize management strategies. Samples were harvested once …

Native Seed Scoop, 2024 Post Harvest Edition, University Of Northern Iowa. Tallgrass Prairie Center.

Native Seed Scoop

Contents:

--- Upcoming Events
--- TPC Highlights
--- What's Your Scoop
--- A New Toolkit for Finding CRP Plant Materials
--- Native Seed Plant Stakeholder Meeting
--- News from the Plots
--- New Collections
--- A Scoop of Science
--- In Other News . . .

Evaluation Of Biochar As A Remediation Tool For Aminopyralid Contaminated Compost, Steven James Parris

Masters Theses & Specialist Projects

Aminopyralid is a synthetic auxin herbicide which is applied to grass pastures to control broadleaf weed populations. Aminopyralid can contaminate compost through inputs derived from treated pastures such as manure and grass clippings. This possibility poses the threat of inadvertent herbicide exposure to sensitive vegetable plants through using compost contaminated with aminopyralid. Biochar can absorb pollutants and has a synergistic effect with compost when applied to the compost mixture. This study examined the relationship between biochar and aminopyralid through compost consisting of dairy manure and sawdust 1:1 mixture, aminopyralid (10μg/kg), and biochar at four treatment levels of total mass of …

Investigate The Possibility Of Producing Healthier, Safer Peanuts Through Molecular Breeding And Novel Fungal Pathogen Management Strategies, Tariq Alam

All Dissertations

Peanut allergy, a critical health concern with high morbidity and mortality rates, is a leading cause of fatal anaphylactic reactions in the United States. Managing peanut allergy is challenging due to the prevalence of peanuts in processed foods and the risk of contamination. This dissertation evaluates the U.S. peanut mini-core collection for four major immunogenic proteins, Ara h1, Ara h2, Ara h3, and Ara h6, using gel electrophoresis, enzyme-linked immunosorbent assay, liquid chromatography, and mass spectrometry. The study identified 26 genotypes with reduced concentrations of allergenic proteins. Genome-wide association studies (GWAS) of protein traits revealed 10 significant SNPs and identified …

Advancing Molecular Tools For The Study Of Rubus Viruses, Andrea Sierra-Mejia

Graduate Theses and Dissertations

Since blackberry yellow vein disease (BYVD) was first described, the blackberry virome has expanded significantly. BYVD is a complex disease associated with over ten viruses, yet the role of each virus in its etiology remains understudied. Studying the effect of each virus in disease development requires the identification of single infections; however, this task has been difficult for BYVD, with all field collected material over the past two decades consistently found infected by multiple viruses. Given the diversity of viruses associated with the disease and their different modes of transmission, obtaining pure virus cultures necessitate transmission of individual viruses via …

Condition And Management Futures In A Quaking Aspen Recreation Area. Final Report To Snyderville Basin Special Recreation District, Paul C. Rogers, Georgie Corkery, Anna B. Miller

Aspen Bibliography

In partnership with Snyderville Basin Special Recreation District (SBSRD), we undertook a study of ecological conditions and user preferences within a quaking aspen recreational destination forest. The location, in two sections know as Run-A-Muk and Right Turn Sage (RAM/RTS), has experienced some forest die-off in the principally aspen dominated forest dissected by ski, bike, and hiking trails. Graduate student Georgie Corkery led the study and successfully completed a Master's degree, in addition to providing needed information to SBSRD regarding the status, uses, and preferred actions at this facility. Key outcomes regarding aspen conditions include assigning causal factors—ungulate browsing and elevated …

Assessment Of Drought Tolerance In Mung Bean [Vigna Radiata (L.) Wilczek] Through Phenology, Growth, Protein Yield, And Cluster Heatmap Analysis, Rafiqul Islam, Jamil Hossain, Shamsul Huda, Mohammad Golam Azam, Faruk Hossain, Aktar Uz Zaman, Safiqul Moula, Nasir Uddin Ahmed, Jamal Hossain, Ayman El Sabagh, Mohammad Sohidul Islum

The Philippine Agricultural Scientist

Mung bean [Vigna radiata (L.) Wilczek] cultivation is challenging under the changing climate as less precipitation during the summer season, which is becoming increasingly common, results in soil moisture deficiency and affects production especially at the early growth stages. Hence, this study was conducted to assess the phenology, growth, and protein yield traits of mung bean related to drought tolerance. Eight mung bean genotypes were grown in pots inside a rain-out shelter under well-watered (WW) and water-deficit (WD) conditions. These genotypes included four water stress-tolerant genotypes: G-1 (BARI Mung-8), G-2 (BMX-010015), G-3 (BMX-08010-2), and G-4 (BMX-08009-7), as well as four …

Morphological And Physiological Changes Of Katuray (Sesbania Grandiflora) During Seed Maturation [Research Note], Alangelico San Pascual, Lucille Elna De Guzman, Maurice Gravidez, Elijah Estante

The Philippine Agricultural Scientist

Katuray (Sesbania grandiflora), whose flowers are consumed as steamed vegetables, is an important green manure crop in the Philippines. Understanding different processes leading to seed maturity can aid in determining S. grandiflora’s seed quality, preventing premature harvesting for seed increase, ensuring efficient propagation and production of seedlings, and breeding new varieties of this crop. Multiple flowers were tagged, and three to five pods were collected five days after anthesis (DAA) and at a five-day interval thereafter until 100% germination was achieved. Seed size, moisture content (MC), dry weight, germination percentage, and embryo development were observed and recorded. Seed length was …

Effect Of Integrated Crop–Livestock Systems On Soil Properties And Microbial Diversity In Soybean Production, Namita Sinha, Brett R. Rushing, Aniruddha Acharya, Shankar Gaapathi Shanmugam

Faculty Publications - Biological Sciences

Integrated crop and livestock systems (ICLSs) have been considered an important management-based decision to improve soil health by carbon sequestration. A two-year study (2019–2021) at CPBES in Newton, MS, was conducted to evaluate the effect of an ICLS on soil microbial diversity in the southeastern region of the USA, representing agroclimatic conditions that are warm and humid. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and characterization of microbial diversity were performed using QIIME2®. Soil fungal diversity pattern showed significant difference (alpha diversity, p = 0.031 in 2020 and beta diversity, p = 0.037 …

Evaluation Of Pierce’S Disease-Resistant Grapevine Cultivars In Upstate South Carolina, Annie Bruno

All Theses

Interest in wine grape production is increasing in South Carolina, but Pierce’s Disease (PD) caused by the xylem-limited bacteria Xylella fastidiosa (Xf) is a major limiting factor. Xf is spread by sap-sucking insect vectors in the Cicadellidae family such as sharpshooters. In the United States, Xf has become endemic to the southeast as global temperatures rise, causing winter temperatures to increase, which allows the bacteria to survive year-round. However, PD-resistant grapevine hybrid cultivars have become available through breeding V. vinifera genes with native American Vitis species that are resistant to Xf. In this study, the PD-resistant grapevine …

Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia, Katherine Riddle

All Theses

Floral color variation within a species is a common occurrence and its maintenance has been a long-standing question in evolutionary biology. Differences in flower color between populations can be shaped by pollinator-mediated selection. Pollinators can exhibit innate and learned preferences for color that may influence floral color variation. These preferences may be molded by the visual environment in which pollinators forage. Most current understanding of how pollinators shape floral color variation involves dramatic differences in dominant pollinators between populations (e.g., hawkmoth vs. bee) and most behavioral work utilized model pollinators like honeybee and bumble bee. However, less is known about …

Soybean Seed Yield Distribution Within The Canopy As Affected By Nitrogen Supply, Lucia Bonfanti

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Soybean [Glycine max (L.) Merr.] seed yield is influenced by the seasonal availability of resources (i.e., nutrients, light, and water). While nitrogen (N) supply is becoming a soybean yield-limiting factor, the N-induced effect in seed yield components is still unclear. The objective of this research was to assess the effect of N supply on soybean yield components distribution within the canopy. A Full-N treatment, which provided the crop with ample N supply, was compared against a Zero-N treatment in which the crop relied on soil N and biological N fixation at nine high-yielding irrigated soybean environments in Nebraska, with …

Leveraging Within-Field Spatial Variability To Optimize Soybean Yield, Seed Quality, And Profitability, Marina Marly Dalla Betta

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Optimizing yield and seed quality in soybean production is critical for both profitability and meeting market demands. However, within-field variability in soil properties poses a challenge in determining optimal management strategies to maximize soybean yield, seed quality, and profitability. This work aimed to evaluate the spatial variability of soybean fields to optimize soybean seeding rates, seed quality, and profitability. Two case studies were used to carry over this research. The first study looked at the optimal economical site-specific soybean seeding rates using 11 on-farm precision experiments across Nebraska. The second study looked at the protein and oil variability within 22 …

Soybean Cyst Nematode (Heterodera Glycines Ichinohe) Virulence Assay Against Resistance Sources In Nebraska, Pratibha Karki

Department of Plant Pathology: Dissertations, Theses, and Student Research

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe), one of the major yield-limiting pathogens of soybean, is currently found in 60 counties of Nebraska that produce more than 90% of the state’s soybean. Often, yield loss occurs without any above-ground symptoms, which makes it difficult to manage. The primary management option is the use of SCN-resistant varieties. Several soybean plant introductions serve as resistance sources against SCN. Unfortunately, the single source, PI 88788, is used in approximately 90% of resistant varieties and its prolonged use has increased the virulence status of SCN in the U.S. A study was conducted to assess …

An Extensive Exploration Of Generalist Microbotryum Species Through Host Interactions., Emilee Walters

College of Arts & Sciences Senior Theses

The Microbotryum species complex is a group of fungi, commonly known as anther smuts. Anther smuts are plant parasites that must invade a host plant in order to fulfill their life cycle. As the Microbotryum fungus grows, it sterilizes the host plant, such that infected plants produce fungal teliospores instead of pollen on the male reproductive organs and female reproductive structures do not mature. Microbotryum usually exists as a specialist species, however, there is evidence that several species of anther smuts can exist as generalist species. Comparisons of the infection process and the resulting response of both parasite and host …

Induced Systemic Resistance Against Nematodes By Plant Growth-Promoting Microbes In Potatoes, Cole Grannan

Master's Theses

Biofertilizers consist of plant growth-promoting microorganisms that establish symbiotic relationships with plant roots and stems. These beneficial microbes play a crucial role in enhancing plant defenses against various threats including insects, nematodes, and microbial pathogens. The purpose of this study was to investigate the effectiveness of two fungal biofertilizers (an endophytic strain of Beauveria bassiana (BA) and a mixture of arbuscular mycorrhizal (AM) fungi), specifically their ability to induce systemic resistance against plant- parasitic nematodes (PPNs) in potatoes (Solanum tuberosum cv. Masquerade) under controlled greenhouse conditions. The study utilized soil collected from Colorado State University’s San Luis Valley (SLV) Research …

Genomic Selection For Dwarf Bunt Resistance In Winter Wheat, William Krause

All Graduate Theses and Dissertations, Fall 2023 to Present

Dwarf bunt (DB), caused by the pathogen Tilletia controversa J.G. Kühn [as 'contraversa'], is a fungal disease that poses yield and grain quality risks to winter wheat (Triticum aestivum) production in the Intermountain West and abroad. The infection replaces the grain with a collection of spores that are spread by wind during the following harvest. In addition to wheat, DB also infects other cereal grains and wild grasses. Genomic selection (GS) is a plant breeding method that can determine percent infection of related varieties by using phenotypic data and genetic data within a prediction model. A panel …

Interpreting The Spatial Distribution Of Soil Properties With A Physically-Based Distributed Hydrological Model, Zamir Libohova, Marcelo Mancini, Hans Edwin Winzeler, Quentin D. Read, Ning Sun, Dylan Beaudette, Candiss Williams, Joshua Michael Blackstock, Sergio H.G. Silva, Nilton Curi, Kabindra Adhikari, Amanda Ashworth, Joshua O. Minai, Phillip R. Owens

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Digital soil maps are commonly data-driven as the development of physically-based models for soil mapping is difficult due to the complexity of soils. However, physically-based hydrologic models have been successful in simulating water dynamics. Since water movement is a major driver of pedogenesis, the physical rules that govern water movement might help explain and predict the spatial variation of soil properties. Here, we demonstrate the novel use of a physically-based, distributed hydrologic model to inform the spatial distribution of soil properties. The Distributed Hydrology Soil Vegetation Model (DHSVM) was utilized to simulate soil moisture content (SM) and water table …

Compact Solar-Powered Plasma Water Generator: Enhanced Germination Of Aged Seed With The Corona Dielectric Barrier Discharger, Yiting Xiao, Yang Tian, Haizheng Xiong, Ainong Shi, Jun Zhu

Biological and Agricultural Engineering Faculty Publications and Presentations

Seed aging adversely affects agricultural productivity by reducing germination rates and seedling vigor, leading to significant costs for seed banks and companies due to the need for frequent seed renewals. This study demonstrated the use of plasma-activated water (PAW), generated by a solar-powered corona dielectric barrier discharger, to enhance germination rates of spinach seeds that had been stored at 4 °C for 23 years. Treating seeds with PAW at 17 kV for 15 min improved germination (by 135%) and seedling growth compared to untreated seeds. Through detailed analysis, beneficial PAW properties for seed development were identified, and a molecular mechanism …

Evaluating The Duckweeds Lemna Minor And Spirodela Polyrhiza As Alternative Aquatic Hosts For The Spread Of The Bacterial Wilt Pathogen, Ralstonia Solanacearum, Irelynn Smith

Honors Theses

Ralstonia solanacearum is a plant pathogen that causes bacterial wilt disease in major crops such as tomatoes and potatoes. Recently, this bacterium was discovered in floating pennywort (Hydrocotyle ranunculoides) and Pennsylvania smartweed (Polygonum pennysylvanicum), two previously unsuspected aquatic plants. Duckweed is an aquatic plant with a high level of resilience and short doubling time. Common duckweed (Lemna minor) has previously demonstrated its capability as a versatile high-yield infection model for other types of pathogenic bacteria. Given this newfound branch of aquatic hosts and the fact that duckweed is a common pond weed previously identified in several ponds located nearby infected …

Field Assessment Of Fertilization, Nursery, And Crop Management Practices Among Tomato (Solanum Lycopersicum L.) Growers In The Ilocos Provinces, Philippines, Annalissa Aquino, Sancho Bon, Angelica Guanlao, Ann Mylalulex Magnaye, Filomena Sta Cruz, Pompe C. Sta. Cruz

The Philippine Agricultural Scientist

Growth and yield of many economically important vegetables like tomatoes are heavily influenced by crop management practices, particularly fertilizer application, irrigation scheme, and pest management. To attain maximum tomato yields and increase farmers’ profits, these crop production components must be optimized in local farm levels. Before optimization measures can be conducted, baseline information on prevailing practices needs to be evaluated. To assess the nutrient management and other crop production practices of tomato farmers in the Ilocos provinces, Philippines, on-farm surveys were conducted in 16 and 18 barangays planting fresh-type and processing-type tomatoes, respectively. The study involved a total of 88 …

Nitrogen Limitation In Soybean: Physiology And Diagnosis, Luzviminda Ann Ramos Sazon

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

In high-yield soybean environments, the seed yield is limited by nitrogen (N) supply. However, the response of soybean to N fertilizer application is inconsistent. Understanding the seasonal N uptake dynamics in soybean can provide insights on diagnosing N limitation if soybean. To understand soybean N dynamics, we assessed seasonal trends on dry matter and N accumulation in vegetative organs (leaves, stems, and petioles) from 42 field experiments. Each experiment included a ‘zero-N’ treatment, where crops relied on N supply from soil and biological N fixation (BNF), and a ‘full-N’ that received ample N via N fertilizer addition. Vegetative storage proteins …

A Nursery Study Of The Interplay Between Soil Texture, Rooting Space, And Nurse Plants On Seedling Growth And Root Development In Woody Species Of Tamaulipan Thornscrub, Lance I. Santos

Theses and Dissertations

Reforestation attempts in semi-arid regions are plagued with seedling mortality. Blanket greenhouse practices may not be optimal, in addition, commensalism interactions between nurse plants are considered. To better understand the effects of soil texture and nurse plant interactions on growth and drought tolerance, two experiments were developed. For soil texture, Border palo verde seedlings were grown in different-sized plant bands with three soil types. To observe the effects of nurse plants, an experiment using guayacan, was developed using Texas Ebony, as a leguminous nurse plant. Plants were also exposed to a drought experiment. The results suggest that some soil textures …

The Effect Of Indaziflam On Seed Production In Perennial Cool-Season Grasses, Benson F. Israelsen

All Graduate Theses and Dissertations, Fall 2023 to Present

Annual invasive grass weeds are a major problem in grass seed production as they contaminate seed lots and decrease the quality. Few effective herbicides are available to control invasive grass weeds in seed production systems. Indaziflam is a pre-emergence herbicide capable of providing annual weed control for 3-5 years after application. Indaziflam is not labeled for use in seed production as little is known of the effect it will have. The purpose of this study was to measure total seed yield, seed germination, seed mass, and seedling vigor when applying 0, 40, 77, and 93 g ai ha^-1 of …

Field Prevalence Of Ring Nematodes In South Carolina And Its Potential To Predispose Peanut Pods To Aflatoxin Contamination, Sarala Giri

All Theses

Peanut ring nematode, Mesocriconema ornatum is a nematode pest of peanut which can cause necrotic lesions on pods, pegs, and roots. It has been reported to facilitate secondary infections in peanut and other crops resulting in more significant losses in yield and quality. Aspergillus flavus is a ubiquitous facultative saprophyte that produces aflatoxin, the most problematic mycotoxin for global health and economy. Experiments were conducted at the Edisto Research and Education Center of Clemson University to examine the relationship of ring nematode populations at pegging and harvest with aflatoxin concentration in peanut kernels due to infection by A. flavus …

Phytoplankton Responses To Changing Irradiance And Carbon Fertilization, Alison Siersma

Graduate Theses and Dissertations (2019 - present)

Phytoplankton play a crucial role in marine ecosystems due to their innate ability to fix carbon. As atmospheric CO2 levels continue to rise from fossil fuel combustion, the resulting increase in dissolved CO2 and the concurrent decrease in ocean pH are likely to impact the phytoplankton community. The response of phytoplankton to elevated CO2 can vary significantly among species and environmental conditions (e.g. light, temperature, nutrient availability). To address these variations, an experiment was conducted using a controlled photobioreactor system, maintaining high and low light, constant temperature, nutrient levels, and two pCO2 concentrations. This study focused on two regionally relevant …

Genetic Diversity And Population Structure Of Tomato (Solanum Lycopersicum) From The Usda-Grin Germplasm Collection, Ibtisam Alatawi, Haizheng Xiong, Hanan Alkabkabi, Kenani Chiwina, Qing Luo, Kai-Shu Ling, Yuejun Qu, Renjie Du, Ainong Shi

Horticulture Faculty Publications and Presentations

The genetic diversity and population structure of tomato (Solanum lycopersicum) were investigated to support breeding strategies and germplasm conservation. A total of 276 accessions from the USDA-GRIN collection were analyzed using single-nucleotide polymorphisms (SNPs) generated through genotyping by sequencing (GBS). After stringent filtering, 5162 high-quality polymorphic SNPs were retained for analysis. Population structure analysis revealed three distinct genetic groups (Q1, Q2, and Q3) among the accessions. Accessions from the USA, Asia, and Central and South America were assigned to all three groups, while accessions from Europe and Oceania were predominantly clustered within Q2. Genetic diversity indices, including major …

Quantifying Temperature Effects On Development Rate And Plant Quality Of Compact Container-Grown Tomato, Morgan Humphrey, Ryan W. Dickson, Sonali Padhye

Horticulture Faculty Publications and Presentations

Mean daily temperature effects on plant development rates and quality were evaluated for compact container-grown tomato (Solanum lycopersicum). Compact tomato varieties ‘Siam’ and ‘Red Velvet’ were grown in greenhouses at 18 to 26 °C (Expt. 1) and 20 to 30 °C (Expt. 2) under supplemental high-pressure sodium lighting and 16-h photoperiod. The number of days to first open flower, first ripe fruit and from flower to ripe fruit were measured and development rates calculated by taking the reciprocal (e.g., 1/d). Temperature effects were predicted by fitting a linear (for first open flower) and a nonlinear exponential function (for …

Responses Of Medical Cannabis To Daily Light Integrals Higher Than Summer Sunlight: Yield, Morphology, And Quality, James D. Nielson

All Graduate Theses and Dissertations, Fall 2023 to Present

Cannabis sativa L. is a crop with growing medical and commercial significance. However, cannabis is not as well studied or understood as many of the most common crops. A novel area of research surrounds how much light the plant can tolerate or use to keep increasing flower yield. In agriculture, light intensity is often measured by how many light particles interact with a given area in a second, referred to as photosynthetic photon flux density (PPFD). For example, at Utah State University in Logan, Utah, during the peak of summer, the sun's light intensity can reach about 2000 μmol·m^{-2 …}

Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia, Jessica Williams

Electronic Theses and Dissertations

Carnivorous plants face significant conservation challenges due to habitat destruction, climate change, and illegal harvesting. This study explores how elevation and population size influence key-life history traits of D. rotundifolia, such as flowering phenology, prey capture efficiency, seed production, and germination rates. Higher elevations and larger populations are associated with extended flowering durations and increased prey capture. However, seed production and germination rates show complex patterns that are likely influenced by genetic diversity, resource availability, or environmental conditions. These insights inform targeted conservation strategies, emphasizing habitat preservation, enhancing prey availability, and maintaining genetic diversity. Public education and community engagement …