Digital Commons Network^™

Identification Of Cyst Nematodes Of Agronomic And Regulatory Concern With Pcr-Rflp Of Its1, Allen L. Szalanski, Dezhi D. Sui, T. S. Harris, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

The first internally transcribed spacer region (ITS1) from cyst nematode species (Heteroderidae) was compared by nuc.leotide sequencing and PCR-RFLP. European, Asian, and North American isolates of five heterodefid species were examined to assess intraspecific variation. PCR-RFLP patterns of amplified ITS1 DNA from pea cyst nematode, Heterodera goettingiana, from Northern Ireland were identical with patterns from Washington State. Sequencing demonstrated that ITS1 heterogeneity existed within individuals and between isolates, but did not result in different restriction patterns. Three Indian and two U.S. isolates of the corn cyst nematode, Heterodera zeae, were compared. Sequencing detected variation among ITS1 clones from …

Molecular And Functional Characterization Of A Novel Low-Affinity Cation Transporter (Lct1) In Higher Plants, Daniel P. Schachtman, Raman Kumar, Julian I. Schroeder, Ellen L. Marsh

Department of Agronomy and Horticulture: Faculty Publications

The transport of cations across membranes in higher plants plays an essential role in many physiological processes including mineral nutrition, cell expansion, and the transduction of environmental signals. In higher plants the coordinated expression of transport mechanisms is essential for specialized cellular processes and for adaptation to variable environmental conditions. To understand the molecular basis of cation transport in plant roots, a Triticum aestivum cDNA library was used to complement a yeast mutant deficient in potassium (K⁺) uptake. Two genes were cloned that complemented the mutant: HKT1 and a novel cDNA described in this report encoding a cation …

Comparative Salt And Waterlogging Tolerance Of An Introduced Grass (Agropyron Elongatum [Host] P. Beauv.) And Two Herbaceous Australian Species (Podolepis Gracilis [Lehm.] And Danthonia Caespitosa Gaud.), Bryan William Duff

Theses : Honours

Increasing land degradation is recognised as an immediate worldwide threat. Human induced soil salinity is probably the major cause of land abandonment, through its adverse effects on plant growth. Salinity is a major focus of environmental researchers because it is recognised that counter-strategies can potentially reclaim both artificially degraded lands and intrinsically saline areas. Currently, strategies to combat salinity require that land use is changed, since restoration to a past use is usually economically impractical or impossible. Biological strategies show most promise. Revegetation of degraded soils with hardy plant species has met with considerable success, and shows promise for the …

New Records For The Distribution Of An Unusual Liverwort, Petalophyllum Ralfsii (Fossombroniaceae), Daniel L. Marsh, Timothy A. Golden, David L. Mcdaniel, Gregory A. Nunley, Winfred D. Crank

Journal of the Arkansas Academy of Science

No abstract provided.

The Linnaean Hierarchy And The Evolutionization Of Taxonomy, With Emphasis On The Problem Of Nomenclature, Kevin De Queiroz

Aliso: A Journal of Systematic and Floristic Botany

During the post-Darwinian history of taxonomy, the Linnaean hierarchy has maintained its role as a means for representing hierarchical taxonomic relationships. During the same period, the principle of descent has taken on an increasingly important role as the basis for reformulated versions of fundamental taxonomic concepts and principles. Early in this history, the principle of descent provided an explanation for the existence of taxa and implied a nested, hierarchical structure for taxonomic relationships. Although an evolutionary explanation for taxa contradicted the Aristotelian context within which the Linnaean hierarchy was developed, the nested, hierarchical structure of taxonomic rela· tionships implied by …

Effects On White Leghorn Hens Of Constant Exposure To Ultraviolet Light From Insect Traps, Jerome Hogsette, Henry Wilson, Susan Semple-Rowland

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Constant exposure of Hy-Line^® W-36 White Leghorn hens to ultraviolet light from insect traps resulted in no significant differences in egg production, fertility, hatchability of fertile eggs, or total hatchability. Also, there were no apparent effects on the eyes of the birds. Results were the same when either blacklight or blacklight blue tubes were used. The need for additional testing of light traps for nuisance fly control in commercial caged layer houses is discussed.

Selenium Speciation Of Soil/ Sediment Determined With Sequential Extractions And Hydride Generation Atomic Absorption Spectrophotometry, Dean A. Martens, Donald L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Understanding the speciation of the multioxidation states of selenium is vital to predicting the mineralization, mobilization, and toxicity of the trace element in natural systems. Asequential extraction scheme (SES) was developed for identification of Se oxidation states that first employed 0.1 M (pH 7.0) K₂HPO₄-KH₂PO₄ (P-buffer) to release soluble selenate (Se^+VI) and selenide (Se^-II) and ligand-exchangeable selenite (Se^+IV). The second step involved oxidation of organic materials with 0.1 M K₂S₂O₈ (90 °C) to release Se^-II and Se^+IV associated or occluded …

Resource Management Decision Support Tools - Brief Model Descriptions, Campbell White & Associates Pty Ltd

Farm Systems and Economics published reports

During the past 10 years, a number of financial models have been developed within Agriculture Western Australia to assist decision making in resource management. Various approaches have been taken including whole farm, development and partial budgets.

Natural resource managers within Agriculture Western Australia, have identified a need to preserve these decision support systems in a central library. The models should be readily accessible to new staff, and the library should provide an 'engine' through which the models can be promoted, and information relating their application distributed. Recent technology such as the development and mainstream adoption of the World Wide Web …

Influence Of A Mycorrhizal Fungus And/Or Rhizobium On Growth And Biomass Partitioning Of Subterranean Clover Exposed To Ozone, Joseph E. Miller, Steven R. Shafer, Michele M. Schoeneberger, Walter A. Pursley, Stephanie J. Horton, Charles B. Davy

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The influence of soilborne symbionts such as rhizobia or mycorrhizal fungi on plant response to ozone (O₃) has not been well defined. Leguminous plants in the field are infected by both types of organisms, which influence plant nutrition and growth. We studied the effects of infection with Rhizobium leguminosarum biovar trifolii and/or Gigaspora margarita on response of subterranean clover (Trifolium subterraneum L. cv Mt. Barker) to O₃. Exposures were conducted in greenhouse CSTR chambers using four O₃ concentrations [charcoal-filtered (CF), 50, 100, or 150 ppb; 6 h day^-1, 5 day wk^{-1 …}

The Rdna Internal Transcribed Spacer Region As A Taxonomic Marker For Nematodes, Thomas O. Powers, T. C. Todd, A. M. Burnell, P. C. B. Murray, C. C. Flemming, Allen L. Szalanski, B. A. Adams, T. S. Harris

Department of Plant Pathology: Faculty Publications

The ITS region from a wide taxonomic range of nematodes, including secernentean and adenophorean taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITS1 is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that …

Soil Temperature And Fumigation Effects On Plant Phosphorus Uptake And Related Microbial Properties, Dennis L. Mccallister, L. A. Jawson, M. D. Jawson

Department of Agronomy and Horticulture: Faculty Publications

Early season problems with growth of corn (Zea mays L.) under cool, wet conditions prompted a study of the effects of soil and environmental conditions on mineralization and plant uptake of phosphorus (P). Our objective was to determine the effect of soil test P, temperature, and soil fumigation on soil P availability and uptake during early corn growth. Corn was grown in growth chambers at temperatures of 14°C or 25°C. Soils were a high-P Hastings silty clay loam (fine, montmorillonitic, mesic Udic Argiustoll) and a low-P Sharpsburg clay loam (fine, montmorillonitic, mesic Typic Argiudoll). Plants grew for up to …

Evolutionary Implications Of Meiotic Chromosome Behavior, Reproductive Biology, And Hybridization In 6x And 9x Cytotypes Of Andropogon Gerardii (Poaceae), Guillermo A. Norrmann, Camilo L. Quarín, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

Andropogon gerardii, big bluestem, has 60 and 90 chromosome cytotypes. Meiosis in the hexaploid was shown to be regular, although some secondary associations of bivalents form. Meiosis in the enneaploid (2n = 9x = 90) is irregular, leading to most gametes having unbalanced chromosome complements. Both cytotypes show considerable self-incompatibility. Cytotypes crossed freely, forming a variety of fertile euploids and aneuploids. Indistinguishable exomorphology, intermixing in natural populations, and compatibility suggest that A. gerardii is best understood as a cytotypically complex single species.

Notes: Forage Yield, Quality, And Fertility Of Sorghum X Sudan Grass Hybrids In A1 And A3 Cytoplasm, Jeffrey F. Pedersen, J. J. Toy

Department of Agronomy and Horticulture: Faculty Publications

Currently, no information is available comparing the agronomic performance of A1 and A3 cytoplasms in sorghum x sudangrass [Sorghumbi color (L.) Moench] hybrids. The objectives of this study were to compare the effects of A1 and A3 cytoplasm on the maturity, fertility, height, forage yield, and forage quality of sorghum × sudangrass hybrids. In 1989, pollen from a bulk of eight sudangrass populations was used to pollinate four sorghum lines that had been male-sterilized in both A1 and A3 cytoplasm. Hybrids were grown at the Univ. of Nebraska Field Laboratory Ithaca, NE, in 1990 and 1991, in a split-plot …

Expression Of Follistatin And Inhibin/Activin Subunit Genes In Porcine Follicles, M.D. Li, L.V. Depaolo, J.J. Ford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Expression of the follistatin (FS) and inhibin/activin (I/A) α, β_A, and β_B subunit genes in porcine ovarian follicles was evaluated by reverse transcriptase polymerase chain reaction and/or RNase protection procedures to establish changes during the final stages of follicular development. For the I/A α and β_A subunits, expression increased (p < 0.05) as follicles progressed to the mid-stage of the follicular phase. The β_B, subunit was expressed in lower concentrations, and all three I/A subunits showed a marked reduction (p < 0.01) in expression by the late stage of follicular development. In contrast to this pattern, FS gene expression decreased (p < 0.05) as follicles developed from the early (low estradiol) to the mid stage (high estradiol) and continued to decline in advanced follicles (after estrus). The predominant mRNA encoded for FS-315, and the ratio of mRNA for FS-315 to mRNA for FS-288 did not differ significantly during the three stages. Within an animal, concentration of FS mRNAs was related more to stage of the follicular phase than to follicular size. Follicular fluid concentration of FS changed in a manner similar to that observed for expression of its gene. We conclude that expression of the FS gene and translation of its mRNA decrease as follicles approach ovulatory status.

Yield Of Wheat Across A Subambient Carbon Dioxide Gradient, Herman S. Mayeux, Hyrum B. Johnson, H. Wayne Polley, Stephen R. Malone

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Yields and yield components of two cultivars of day-neutral spring wheat (Triticum aestivum L.) were assessed along a gradient of daytime carbon dioxide (CO₂) concentrations from about 200 to near 350 µmol CO₂ (mol air)^–1 in a 38 m-long controlled environment chamber. The range in CO₂ concentration studied approximates that of Earth’s atmosphere since the last ice age. This 75% rise in CO₂ concentration increased grain yields more than 200% under well-watered conditions and by 80–150% when wheat was grown without additions of water during the last half of the 100-day growing season. …

Genetic Variation In North American Leafy Spurge (Euphorbia Esula) Determined By Dna Markers, Martha L. Rowe, Donald J. Lee, Scott Nissen, Brunella M. Bowditch, Robert A. Masters

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Levels of inter- and intrapopulation genetic variation were determined in five North American populations of leafy spurge using chloroplast DNA (cpDNA) RFLPs and RAPD markers. Thirteen plastome types were identified among 123 individuals collected from five geographically separated populations. Number of pastimes within a population ranged from one to seven, with four of the populations having a predominate type plus one or more rarer types. Some plastome types were shared by populations, but plastome distribution among populations was nonrandom. RAPD markers indicated greatest relatedness among individuals within a population. Relatedness among populations as established through RAPDs was greater for geographically …

Environmental Requirements For Sporocarp Germination In Marsileaceae, Kevin J. Kruep

Masters Theses

Experiments were conducted on sporocarps of Marsilea quadrifolia L. to determine whether temperature had any influence on the length of the reproductive period and initial sperm emergence from microgametophytes. Data were then compared to results of similar studies undertaken with the species M. vestita Hooker and Greville to determine whether there was any variation between them. A slight variation was recorded, ranging from 1 to 1.5 hours for initial emergence.

An additional set of experiments was performed with 58 year old sporocarps and approximately two-year-old sporocarps of M. quadrifolia. A difference of 2-6.5 hours for initial sperm emergence occurred between …

Twenty-Five Year Index To The Prairie Naturalist, Volumes 1-25, 1968-1993, Paul B. Kannowski

The Prairie Naturalist

Forward

Author Index

1.Articles and Notes

2. Book Reviews

3. Editorials

Subject Index

Geographic Index

Taxonomic Index

Plants

Animals

Other Records

A Study Of The Lichens At Rocky Branch Nature Preserve, Clark County, Illinois, Eric B. Grunder

Masters Theses

In 1971, a graduate student at Eastern Illinois University, James E. Wiedman, studied the lichen flora of Rocky Branch Nature Preserve, Clark County, Illinois. During his study he collected, identified, and recorded a total of 64 lichen species. Since 1971, Rocky Branch Nature Preserve has experienced a dramatic increase in use by people and a concomittant decline in the quality of lichen habitats. In order to assess the decline of suitable lichen habitats and the reduction of lichen species a second study of the lichens at Rocky Branch Nature Preserve was undertaken. As part of this study, ten habitat sites …

Suitability Of West African Gramineous Stemborers For The Development Of Cotesia Species, Y. Hailemichael, F. Schulthess, J. W. Smith, W. A. Overholt

All Peer-Reviewed Publications

The palaeotropical, gregarious, endoparasitic braconids Cotesia sesamiae, Cotesia flavipes and Cotesia chilonis were recently imported into Benin as candidates for biological control of stem and cob borers of maize. Host acceptability and host suitability of six gramineous borers occurring in western Africa, namely the noctuids Sesamia calamistis, Sesamia poephaga, Busseola fusca and the pyralids Contesta ignefiisalis, Eldana saccharina and Mussidia nigrivenella, to these candidate parasitoids was evaluated in order to gain insight into the physiological suitability of the natural and factitious hosts. All hosts were accepted by all Cotesia spp., except M. nigrivenella which was not attacked by Co. chilonis. …

The Nutritional Characterization Of The Carob Tree In The Department Of Cochabamba, Karem Khaterine Loma Mercado

Theses and Dissertations

The nutritional value for the following species have been determined: P.pallida, P.laevigata var. andicola, P.flexuosa, P.nigra, P.alba of the genus Proposis. To obtain this purpose surveys were done about the use and consumption of the Carob Tree in the High and Central Valleys of the Cochabamba Department. As a result of the surveys it was determined that the consumption of the pods made up for 54% of human nourishment. The species that were consumed the most were P.pallida and P.laevigata var. andicola. Within the uses of these leguminous pods, the pods are directly consumed in their raw form and on …

Macrophyte-Macroinvertebrate Interactions In A Lentic Ecosystem And The Effect Of Fluridone Treatment To Control Myriophyllum Spicatum L., Gregory J. Moeller

Dissertations and Theses @ UNI

Myriophyllum spicatum L. is an exotic macrophyte that can become pestiferous in lentic ecosystems. Two field studies were conducted to investigate: 1) epiphytic macroinvertebrates associated with M. spicatum and native macrophytes; and 2) epiphytic macroinvertebrate community response to fluridone treatment for M. spicatum control.

In the first study evaluating epiphytic macroinvertebrates associated with M. spicatum and native macrophytes, triplicate samples were collected at three sites in both Auburn and Zumbra Lakes, Minnesota, USA. One site in each lake contained primarily M. spicatum, the second site contained M. spicatum and native vegetation, and the third site was dominated by native …

A Floristic Study Of The Republican River Valley In Nebraska With Emphasis On Endangered, Threatened, And Rare Plants, Steven J. Rothenberger

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The objectives of this study were: (1) to survey the Republican River Valley and selected tributaries in Nebraska for rare plant species (as listed by the Nebraska Natural Heritage program) and determine the present status of these species; (2) to identify and describe potential natural areas that, because of their location and/or species composition, are thought to have ecological value; (3) to complie a plant species check list for the study area in order to better understand species distributions and associations that impact rare species; and (4) to speculate as to the effect of instream river flows on the distribution …

Evaluation Of The Success Of Constructed Wetlands In The Cave Run Lake Watershed, April Diane Haight

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by April Diane Haight on December 10, 1996.

The Evaluation Of Two Types Of Multivariate Analyses Applied To Grassland Vegetation Data From A Reclaimed Coal Mine Area In Southeast Kansas, Usa, Karen Frances Yates

Electronic Theses & Dissertations

This study presents an analysis of the use of classification and ordination techniques for the detection of sub-communities at a reclaimed coal refuse dump and strip minded area in southeast Kansas. Cover values for 58 grass, forb, and woody species were estimated in 103 systematically positioned plots across the reclamation grassland using the modified Daubenmeyer cover scale. The data were submitted to an array of default and variant classification and ordination analysis programs in order to assess their ability to detect sub-communities. The classifications obtained from the analyses were mapped and analyzed for differences in species composition, diversity, and spatial …

The Prairie Naturalist Volume 28, No. 4. December 1996

The Prairie Naturalist

IMPLANTED MICROCHIPS USED TO INDIVIDUALLY IDENTIFY BLACK-FOOTED FERRETS IN MONTANA ▪ R. Stoneberg

GRAY WOLF STATUS IN NORTH DAKOTA ▪ D. S Licht and L. E. Huffman

VARIATION IN SELECTION OF MICROHABITATS BY MERRIAM'S TURKEY BROOD HENS ▪ M. A. Rumble and S. H. Anderson

SURVIVAL AND REPRODUCTIVE CHRONOLOGY OF FEMALE RING-NECKED PHEASANTS IN SOUTH DAKOTA ▪ A. P. Leif

EFFECTS OF LOGGING SLASH ON ASPEN REGENERATION IN GRAZED CLEARCUTS ▪ M. A. Rumble, T. Pella, J. C. Sharps. A. V. Carter, and J. B. Parrish

BOOK REVIEWS

The Poetry of Ecoregions ▪ J. Pastor

Saving Texas' Rare Ones ▪ …

Helminths Of The Southwestern Toad, Bufo Microscaphus, Woodhouse's Toad, Bufo Woodhousii (Bufonidae), And Their Hybrids From Central Arizona, Stephen R. Goldberg, Charles R. Bursey, Keith B. Malmos, Brian K. Sullivan, Hay Cheam

Great Basin Naturalist

The gastrointestinal tracts, lungs, and urinary bladders from 77 Bufo microscaphus, 61 Bufo woodhousii, and 8 of their hybrids were examined for helminths. One species of trematode (Glypthelmins quieta), 1 species of cestode (Distoichometra bufonis), and 5 species of nematodes (Aplectana incerta, A. itzocanensis, Rhabdias americanus, Physaloptera sp., and Physocephalus sp.) were found. The greatest prevalence (41%) and mean intensity (231.7) were recorded for Aplectana incerta in Bufo woodhousii. It appears hybrids harbor fewer parasites than either parent species.

Cross-Polarized Magic-Angle Spinning 13c Nuclear Magnetic Resonance Spectroscopic Characterization Of Soil Organic Matter Relative To Culturable Bacterial Species Composition And Sustained Biological Control Of Pythium Root Rot, Michael J. Boehm, Tianyun Wu, Alex G. Stone, Bart Kraakman, Donna A. Iannotti, G. Edwin Wilson, Laurence V. Madden, Harry A.J. Hoitink

Department of Plant Pathology: Faculty Publications

We report the use of a model system that examines the dynamics of biological energy availability in organic matter in a sphagnum peat potting mix critical to sustenance of microorganism-mediated biological control of pythium root rot, a soilborne plant disease caused by Pythium ultimum. The concentration of readily degradable carbohydrate in the peat, mostly present as cellulose, was characterized by cross-polarized magic-angle spinning 13C nuclear magnetic resonance spectroscopy. A decrease in the carbohydrate concentration in the mix was observed during the initial 10 weeks after potting as the rate of hydrolysis of fluorescein diacetate declined below a critical threshold level …

Nebline, November 1996

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Holiday food safety
Lancaster County Extension Educator recognized
Over the garden fence
Snow molds
Harvesting and storing winter squash
November garden hints
Winterizing roses
Winter care for strawberry plants
Harvesting popcorn
"Preparing Ag Professionals for Tomorrow's Challenges"
Emergency conservation program
1996 pesticide container recycling program nets over 17,000 containers
Beef Basics Home Study Courses
Precision Decisions '96
Report illegal dumping!
Household hazardous waste collection on November 2
The aluminum age
Wood for your fireplace
Which spider's the biter? You can't tell from looking at the bite!
Bt corn: environmentally friendly pest control
Cleaning closets? What to do with those unwanted …

Western Australian Salinity Action Plan, Agriculture Western Australia, Department Of Conservation And Land Management, Department Of Environmental Protection, Water And Rivers Commission

Natural resources published reports

Salinity is one of the State's most critical environmental problems, threatening major economic and social consequences. It is preventable and, in the long term, reversible. Complete elimination is not realistic, but we can dramatically reduce its impact.

Salinity is caused by replacing deep-rooted native plants with shallow-rooted crops and pastures. More rainfall passed below the root zone and accumulates as groundwater so that watertables rise. The groundwater mobilises natural salts in the soil as it rises and carries them towards the surface, eventually degrading land and streams.