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Structure Of The Avian Oviduct With Emphasis On Sperm Storage In Poultry, Murray R. Bakst

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The macroanatomy, histology, and fine structure of the avian oviduct is reviewed and related to its role in fertile egg production. The avian oviduct functions as a biological assembly line, beginning sequentially with the deposition of the albumen around the fertilized or unfertilized ovum, then the shell membrane, and lastly, the shell, all within 25 hr of ovulation. While in transit through the oviduct, the fertilized ovum progresses to the pre-gastrulation stage of development.

Biological Control: An Important Component In Integrated Management Of Musca Domestica (Diptera: Muscidae) In Caged-Layer Poultry Houses In Buenos Aires, Argentina, Diana C. Crespo, Roberto E. Lecuona, Jerome A. Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An Integrated Management program to control Musca domestica L. was developed by evaluating cultural + chemical control, cultural + biological control + granular bait (IMP 1), and cultural + chemical + biological control + granular bait (IMP 2) in poultry houses in Argentina. Adult fly density was estimated by a modified Scudder-grid method. Percentage of house fly parasitism was estimated by direct and indirect methods. Spalangia endius Walker and Muscidifurax raptor Girault & Sanders were released at a rate of 10 parasitoids/hen/week. Complete absence of control practices allowed a sustained growth of the fly population. When larvicides and adulticides were …

Purification And Characterization Of Intrauterine Folate-Binding Proteins From Swine, J.L. Vallet, R.K. Christenson, H.G. Klemcke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Folate-binding proteins (FBP) from Day 60 pseudopregnant uterine flushings and Day 60 allantoic fluid were purified by affinity chromatography on folate-Sepharose followed by G–100 Sephadex chromatography. FBP from uterine flushings had a molecular weight of 20 000; the N-terminal sequence was FNWDHXGKMEPAXKRHFXXXTXLYX, which is 72% identical to bovine milk FBP beginning at amino acid 64. Allantoic fluid FBP had a molecular weight of 30 000; and the N-terminal sequence ARAKTDMLNVXMDAKHHKPKPSXED, which is 68% identical to bovine milk FBP starting at amino acid 4. Scatchard analysis of purified allantoic fluid FBP using [³H]folic acid as ligand indicated a dissociation …

A Phylogenetic Analysis Of Heterorhabditis (Nemata: Rhabditidae) Based On Internal Transcribed Spacer 1 Dna Sequence Data, B. J. Adams, A. M. Burnell, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Internal transcribed spacer 1 sequences were used to infer phylogenetic relationships among 8 of the 9 described species and one putative species of the entomopathogenic nematode genus Heterorhabditis. Sequences were aligned and optimized based on pairwise genetic distance and parsimony criteria and subjected to a variety of sequence alignment parameters. Phylogenetic trees were constructed with maximum parsimony, cladistic, distance, and maximum likelihood algorithms. Our results gave strong support for four pairs of sister species, while relationships between these pairs also were resolved but less well supported. The ITS1 region of the nuclear ribosomal repeat was a reliable source of …

Peanut Yellow Spot Virus Is A Member Of A New Serogroup Of Tospovirus Genus Based On Small (S) Rna Sequence And Organization, Satyanarayana Tatineni, S. Gowda, K. Lakshminarayana Reddy, S. E. Mitchell, W. O. Dawson, D. V. R. Reddy

Department of Plant Pathology: Faculty Publications

Peanut yellow spot virus (PYSV) represents a distinct tospovirus species based on serology and nucleic acid hybridization. The sequence of the S RNA was 2 970 nucleotides with 22 nucleotide long inverted repeats (with three mismatches) at the termini. The coding was ambisense with a long open reading frame (ORF) in each strand. The 5'-large ORF (1 440 nucleotides in the viral sense (v) strand) encoded a protein with a predicted size of 53.2 kDa that was identified as the nonstructural (NSs) protein based on 16–21% sequence identity and 42– 48% sequence similarity with other tospoviruses. A 3' ORF (741 …

Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Secondary culture of nontransformed bile duct epithelium has been difficult to achieve. STO feeder cell-dependent secondary cultures of adult pig bile duct cells were established from primary cultures of adult pig liver cells. Adult pig hepatocytes exhibited limited or no replication and were lost from the secondary culture at Passage 3 or 4. In contrast, adult pig bile duct cells replicated and were carried for 4-8 passages in secondary culture. A simple method to produce nearly pure pig intrahepatic bile duct cultures was first to freeze a relatively crude liver cell preparation. Upon subsequent thawing, all hepatocytes and most macrophages …

The ‘‘Foliage Is The Fruit’’ Hypothesis: Complex Adaptations In Buffalograss (Buchloe Dactyloides), John Ortmann, Walter H. Schacht, James L. Stubbendieck, Dennis R. Brink

Department of Agronomy and Horticulture: Faculty Publications

Buffalograss (Buchloe dactyloides) has been described as an excellent fit for Janzen’s ‘‘Foliage is the Fruit’’ (FF) hypothesis, which suggests that large grazing animals ingest and later disperse seeds of some herbs when consuming their foliage. We tested this hypothesis by feeding buffalograss burs and legume seeds to ruminally fistulated beef steers. Our objectives were to determine (1) rumen residence times of buffalograss burs, free buffalograss caryopses and legume seeds; (2) total tract residence times for the three types of propagules; (3) percentage propagule survival after passage and (4) germinability of fed and unfed burs and caryopses. Bur …

Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

Aluminum (Al) has many detrimental effects on plant growth, and shoots and roots are normally affected differently. A study was conducted to determine differences among sorghum [Sorghum bicolor (L.) Moench] genotypes with broad genetic backgrounds for growth traits of plants grown at 0,200,400,600, and 800 uM Al in nutrient solutions (pH 4.0). Genotypes were categorized into "Al-sensitive", "intermediate Al-tolerant", "Al-tolerant", and SC 283 (an Al-tolerant standard). As Al increased, shoot and root dry matter (DM), net main axis root length (NMARL), and total root length (TRL) became lower than controls (0 Al). Aluminum toxicity and/or nutrient deficiency symptoms become …

Population Biology Of Intraspecific Polyploidy In Grasses, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

Polyploidy is the duplication of an entire nuclear genome, whether diploid or higher level (Stebbins, 1971; Thompson and Lumaret, 1992) and a frequent occurrence in plants. Stebbins (1971) estimated that 30-35% of flowering plant species are polyploid, and that many more had a polyploid event in their evolutionary history, including all members of such important families as the Magnoliaceae, Salicaceae, and Ericaceae. Goldblatt (1980) estimated 55%, but probably up to 75%, of monocotyledons had at least one polyploid event in their history, using the criterion that if the species has a base number higher than n = 13 it is …

Atrazine, Deethylatrazine, And Deisopropylatrazine Persistence Measured In Groundwater In Situ Under Low-Oxygen Conditions, Sharon Papiernik, Roy Spalding

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The degradation of atrazine [2-chloro-4-(ethylamino)-6-isopropylamino-1,3,5-triazine], deethylatrazine [DEA; 2-amino-4-chloro-6-(isopropylamino)-1,3,5-triazine], and deisopropylatrazine [DIA; 2-amino-4-chloro-6-(ethylamino)-1,3,5-triazine] was assessed under limited oxygen conditions using in situ microcosms. Denitrification was induced in a shallow sand and gravel aquifer to measure the potential for degradation of atrazine, DEA, and DIA under low-O2 conditions. The dissolved oxygen content decreased from 7-8 mg/L to ≤1 mg/L within 4 days and remained ≤ mg/L for the remainder of the 45-day experiment. Atrazine, DEA, and DIA concentrations (normalized to the bromide concentration at each sampling time to account for dilution) did not show a significant decrease with time, indicating that these …

A Solid Phase Extraction System For The Isolation Of The Germination Cue In Smoky Water When Subjected To The Light Sensitive Lettuce Lactuca Sativa V. Grand Rapids, Troy David Adriansz

Theses : Honours

The search for the chemical or set of chemicals that act as a germination stimulant in Lactuca sativa v. Grand rapids (a variety of lettuce seed) was the underlying basis of this project. Through the development of a solid phase extraction (SPE) system different fractions of smoky water were tested for their effects on germination enhancement. The project targeted streamlining the amount of solvents as well as time used in past research on smoky water. Previously, several steps involving high perfo1mance liquid chromatography (HPLC), thin layer chromatography (TLC) and Gas Chromatography (GC) were employed. This project used SPE cartridges to …

Influence Of Ground Cover On Spider Populations In A Table Grape Vineyard, Michael J. Costello, Kent M. Daane

Horticulture and Crop Science

1. Cover crops and/or resident ground vegetation have been used in California vineyards to increase the number of predators and decrease the number of pestiferous herbivores. The most common resident predators in vineyards are spiders (Araneae). Several observational studies suggest that the addition of cover crops results in an increase in spider density and a decrease in insect pest densities. 2. To test experimentally the effects of cover crops and/or resident ground vegetation (hereafter collectively referred to as ground cover) on spider populations, a 3-year study was undertaken in a commercial vineyard. Large, replicated plots were established with and without …

Seedling Development In Species Of Chamaesyce (Euphorbiaceae) With Erect Growth Habits, W. John Hayden, Olga Troyanskaya

Biology Faculty Publications

Seedling development is described for Chamaesyce hirta, C. hypericifolia, and C. mesembrianthemifolia as discerned by light microscopy and scanning electron microscopy. Although these species ultimately develop erect to ascending growth habits, epicotyl development is limited to the production of a single pair ofleaves located immediately superjacent to and decussate with the cotyledons. The shoot system develops from one or more buds located in the axils of the cotyledons. In all respects, seedling ontogeny is very similar to that of previously studied prostrate species of Chamaesyce. Evidence from seedling ontogeny thus contradicts a hypothesis concerning homologies of plant …

New Floristic Records For The Grid Square C_3(Akseki-Antalya-Turkey), Ahmet Duran, Hayri Duman

Turkish Journal of Botany

No abstract provided.

Interactive Effects Of Vernalization, Day Length And Light Intensity On The Number Of Leaves And Flag Leaf Area In Some Wheat Cultivars, Yasemin Ekmekçi̇, Serpil Terzi̇oğlu

Turkish Journal of Botany

The interactive effects of vernalization, day length and light intensity on the number of leaves and flag leaf area on the main shoot of five bread wheat ( Triticum aestivum L.) cultivars (Çukurova-86, Atay-85, Lancer, Haymana-79 and Bezostaya-1) each with different biological characters, were investigated. The research was carried out in four independent experiments which were the combinations of two different day lengths (14 and 18 hour day -1 ) and light intensities (13500 and 33750 lux) and vernalization periods. Vernalization treatment was applied for 0, 15, 30, 45 and 60 days at 2±1°C in a dark, cold room at …

Inactivation Of Soybean Peroxidase During Sodium Azide Oxidation: A Comparative Study, Mohamed Yehia Zakaria Aboul Eish

Dissertations and Theses @ UNI

Azide ion is a mechanism-based inactivator of a number of peroxidases. However, there are often variations in the manner in which inactivation occurs. Soybean Peroxidase (SBP) is found in the plant's seed coat. Unlike many other peroxidases, a single enzyme rather than several isozymes are produced thus making more simple the purification and characterization of the enzyme. In this research it was shown that, as with other peroxidases, SBP is inactivated by azide during turnover. During azide oxidation SBP forms a ferrous-NO intermediate. In this respect, SBP is similar to the lignin peroxidases from Phanerochaete chrysosporium which also form ferrous-NO …

New Floristic Records For C6 Grid Square, Ömer Varol, Cemil Kara, Ahmet Ilçim

Turkish Journal of Botany

No abstract provided.

An Autecological Study On Iris Pseudacorus L. (Iridaceae), Ali Engi̇n, Nezahat Kandemi̇r, Gülcan Şenel, Mustafa Özkan

Turkish Journal of Botany

The ecological properties of Iris pseudacorus L., a marsh plant species, were investigated. The analysis of N%, P% and K% in the below-ground and above-ground parts of the plant was carried out. In addition, soil samples were collected and their physical and chemical aspects were analyzed.

Hyaluronan Synthase Of Chlorella Virus Pbcv-1, Paul L. Deangelis, Wei Jing, Michael V. Graves, Dwight E. Burbank, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Sequence analysis of the 330-kilobase genome of the virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases. Hyaluronan is an essential polysaccharide found in higher animals as well as in a few pathogenic bacteria. Expression of the A98R gene product in Escherichia coli indicated that the recombinant protein is an authentic hyaluronan synthase. A98R is expressed early in PBCV-1 infection and hyaluronan is produced in infected algae. These results demonstrate that a virus can encode an enzyme capable of synthesizing a carbohydrate polymer and that hyaluronan exists outside of …

The Prairie Naturalist Volume 29, No.4. December 1997

The Prairie Naturalist

BUTTERFLY SPECIES IN NATIVE PRAIRIE AND RESTORED PRAIRIE ▪ D. M. Debinski and A. M. Babbit

BACK-CALCULATED LENGTH-AT-AGE ESTIMATES FROM TWO SCALE RADII ▪ K. L. Hurley, K. L. Pope, and D. W. Willis

DIET OF NORTH DAKOTA ELK DETERMINED FROM RUMEN AND FECAL ANALYSES ▪ R. G. Osborn, J. A. Jenks, and W. F. Jensen

INTERSPECIFIC COMPETITION FOR FOOD BETWEEN WHITE-FOOTED MICE AND EASTERN WOODRATS ▪ B. G. Aloiau, D. M. Post, and E. A. Home

NESTING PIPING PLOVER AND LEAST TERN ON THE KANSAS RIVER ▪ W. H. Busby, D. W. Mulhern, P. G. Kramos, and D. A. …

The Biology Of Silvopastoralism, Steven H. Sharrow

Agroforestry Notes (USDA-NAC)

Forestry and livestock grazing are common competing uses for non-irrigated lands around the world. Forest grazing is a traditional practice in most of North America. Approximately 25 percent of all US forest land is grazed by livestock. Young forests and mature open-canopied forests can provide considerable amounts of forage for livestock. Prescription grazing makes beneficial use of ground vegetation that would otherwise compete with trees for soil moisture and nutrients, harbor gnawing rodents, and pose a fire hazard. Pasture grasses and legumes are sometimes seeded into newly harvested forest areas to reduce erosion, to provide high-quality food for deer and …

Growing Degree-Days: One Equation, Two Interpretations, Gregory S. Mcmaster, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat units, expressed in growing degree-days (GDD), are frequently used to describe the timing of biological processes. The basic equation used is GDD = [(T_MAX + T_MIN)/2] - T_BASE where T_MAX and T_MIN are daily maximum and minimum air temperature, respectively, and T_BASE is the base temperature. Two methods of interpreting this equation for calculating GDD are: (1) if the daily mean temperature is less than the base, it is set equal to the base temperature, or (2) if T_MAX or T_MIN < T_BASE they are reset equal to T …

Cdna Encoding A Reca Homolog In Eukaryotes, Andre Jagendorf, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

A nucleotide and deduced amino acid sequence of a cDNA encoding the Arabidopsis thulium RecA protein is disclosed.

The Prospect For An Asparagus Industry In Northern Western Australia, John Bonnardeaux

Horticulture published reports

Asparagus grown in temperate regions of southern Australia is available, fresh, between August and March. For the remainder of the year, asparagus is not grown in Australia on a significant scale.

Recent work in Thailand, Peru, Kununurra (northern-western Australia) and Katherine (Northern Territory) has indicated that good quality asparagus can be produced in tropical and subtropical climates. Furthermore, research has shown the timing of the harvest can be manipulated by withholding irrigation and fern cutting. In an environment such as the Ord River Irrigation Area (ORIA) it is possible to harvest asparagus at any time of the year.

This report …

Center For Grassland Studies Newsletter, Fall 1997, Volume 3, No. 4

Center for Grassland Studies: Newsletters

Contents:

The Nebraska Gap Analysis Project: Geographic Information for Land Resource Managers by Marlen Eve and James Merchant, Conservation and Survey Division, University of Nebraska-Lincoln

1998 Nebraska Range Shortcourse by Lowell Moser, Department of Agronomy, University of Nebraska-Lincoln

The Konza Prairie: Our Tallgrass Neighbors by Rob Peters, formerly with The Land Institute, Salina, Kansas

Festival of Color Draws 9,000 This Year

Nebline, October 1997

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Celebrate Character Counts! week
Books and activities celebrating the Six Pillars of Character
Caring for fuchsia
Garden poster contest for youth
Potbound
Harvesting popcorn
Clean up that garden
Last 1997 Household Hazardous Waste Collection
Web sights
There's a mouse in the house!
Interested in a variety of experiences?
Consider estate settlement costs early
No-till into alfalfa residual
Farmers need to protect themselves, employees from work-related illnesses
Fall clean-up of warm season grasses
Handling wastewater
Proper electrical wiring helps prevent most farm fires
CRP: blessing or curse?
Food Safety Programs
Safe Food for the Hungry '97--A Focus on Diversity
Diabetes control …

Identification Of The Wheat Curl Mite As The Vector Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen

Department of Plant Pathology: Faculty Publications

Wheat with virus-like symptoms (extracts containing a 33-kDa protein in sodium dodecyl sulfate- polyacrylamide gel electrophoresis, negative in enzyme-linked immunosorbent assay to wheat streak mosaic virus, and not infectious in a backassay to other wheat) reacted positively to antiserum made against a protein purified from symptomatic corn infected with the High Plains virus (HPV), indicating a serological relationship between the corn and wheat pathogens. The wheat curl mite (WCM, Aceria tosichella Keifer) was identified as the vector of the virus and caused persistent infection of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) in greenhouse experiments. The …

Herbicide Evaluation In Arkansas Rice, 1996, Eric Webster, Ron Talbert, Ford Baldwin, David Gealy, Tomilea Dillon, Jason Norsworthy, Lance Schmidt, Dwayne Beaty

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

The Prairie Naturalist Volume 29, No.3. September 1997

The Prairie Naturalist

FISHING STATISTICS IN KANSAS PUT-AND-TAKE RAINBOW TROUT FISHERIES ▪ R. D. Schultz and D. D. Nygren

DISTRIBUTIONAL STATUS Of UNCOMMON FISHES AND AN AMPHIBIAN FROM NORTHEASTERN MISSOURI ▪ R. A. Hrabik,

DIET OF A RELICT POPULATION OF THE EASTERN WOODRAT IN NEBRASKA ▪ H. H. Genoways, P. W Freeman, and M. K. Clausen

ANIMAL SPECIES MONITORED BY THE SOUTH DAKOTA NATURAL HERITAGE PROGRAM ▪ E. D. Stukel and D. C. Backlund

Dedication

Announcements

Asymbiotic In Vitro Seed Germination, Micropropagation And Scanning Electron Microscopy Of Several Temperate Terrestrial Orchids (Orchidaceae), Erika Szendrák

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Twenty-five different orchid species were successfully asymbiotically germinated and raised on a modified FAST medium (Fast 1976; Szendrák and R Eszéki, 1993). The development of geminating protocorms and young plantlets were recorded and compared among species. Spontaneous vegetative proliferation was also observed. Natural dispersed daylight and prevailing day-length were more favorable than l6-hour cool white fluorescent light for plantlet development. After two to three years of culture, the young plants were suitable for transfer ex vitro.

The effects of organic compounds most commonly used for orchid micropropagation (peptone, coconut water, casein+lactalbumin and glucose) and medium consistencies were investigated for …