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Seed Mass And Morphology In Outcrossing And Selfing Species Of Clarkia (Onagraceae): An Sem Study, Jennifer L. Knies, Veronique A. Delesalle, A. R. Cavaliere Jan 2004

Seed Mass And Morphology In Outcrossing And Selfing Species Of Clarkia (Onagraceae): An Sem Study, Jennifer L. Knies, Veronique A. Delesalle, A. R. Cavaliere

Biology Faculty Publications

Seeds from three pairs of outcrossing-selfing sister taxa from the genus Clarkia (farewell-to-spring, Onagraceae)—Clarkia unguiculata, Clarkia exilis, Clarkia xantiana ssp. xantiana and ssp. parviflora, and Clarkia concinna ssp. concinna and ssp. automixa—were studied to assess the effects of contrasting mating systems on seed mass and seed morphology. For each outcrossing-selfing comparison, the seed mass of the selfing taxon was less than that of the outcrossing taxon. Seed mass typically differed significantly among populations within a taxon. Scanning electron microscopy showed that the seeds from all these taxa share several characteristics: a bullet to shield shape, a reticulate exotesta pattern, presence …


Manure Matters, Volume 10, Number 1, John Sweeten, Ron Miner, Carrie Tengman Jan 2004

Manure Matters, Volume 10, Number 1, John Sweeten, Ron Miner, Carrie Tengman

Manure Matters (newsletter)

A Brief History and Background of the EPA CAFO Rule

U.S. Environmental Protection Agency (EPA) rules affecting animal feeding operations (AFOs) have evolved from the 1972 Federal Clean Water Act (CWA). Section 502 of the CWA specifically defined “feedlots” as “point sources” along with dozens of other industries such as meat processing and fertilizer manufacturing. The goal of the 1972 CWA was to restore “fishable, swimmable” quality of lakes, streams, and estuaries in the United States.
A federal permit program termed the National Pollutant Discharge Elimination System (NPDES) was created for point sources that discharged into “waters of the United …


Population Genetic Structure In Nolina Brittoniana (Agavaceae), A Plant Endemic To The Central Ridges Of Florida, Rebecca W. Dolan, Rebecca Yahr, Eric S. Menges Jan 2004

Population Genetic Structure In Nolina Brittoniana (Agavaceae), A Plant Endemic To The Central Ridges Of Florida, Rebecca W. Dolan, Rebecca Yahr, Eric S. Menges

Scholarship and Professional Work - LAS

Nolina brittoniana is endemic to the central ridges of peninsular Florida. Its scrub and sandhill habitats have suffered extensive anthropogenic modification. Analysis of isozymes from populations throughout its range revealed less genetic variation than generally reported for endemic plants. Populations were well differentiated, with significant clines in allele frequency along the north-south axis of distribution. Pair-wise F-statistics calculated at four levels of population geographic substructure revealed that current and inferred historical habitat patches had similar genetic structure. We found no evidence of recent bottlenecks or changes in genetic structure due to habitat loss and fragmentation, consistent with populations having always …


Structural Attributes Of Schizachyrium Scoparium In Restored Texas Blackland Prairies, Justin D. Derner, H. Wayne Polley, Hyrum B. Johnson, Charles R. Tischler Jan 2004

Structural Attributes Of Schizachyrium Scoparium In Restored Texas Blackland Prairies, Justin D. Derner, H. Wayne Polley, Hyrum B. Johnson, Charles R. Tischler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Structural attributes of the C4, perennial bunchgrass Schizachyrium scoparium in restored prairies may be affected by the time since restoration. One hundred plants each in 8-, 17-, and 23-year-old restored prairies and a native Texas Blackland prairie were assessed for the presence/absence and diameter of a hollow crown (i.e., dead center portion), degree of fragmentation, plant height, and tiller density.


Determination Of Dimethylselenide And Dimethyldiselenide By Gas Chromatography–Photoionization Detection, William J. Hunter, L. David Kuykendall Jan 2004

Determination Of Dimethylselenide And Dimethyldiselenide By Gas Chromatography–Photoionization Detection, William J. Hunter, L. David Kuykendall

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A simple method for the determination of volatile selenium compounds employing a gas chromatograph equipped with a photoionization detector is described. The method involves the direct injection of dimethylselenide (DMS) or dimethyldiselenide (DMDS) into the gas chromatograph; no derivatization of the sample was required. The photoionization detector was capable of detecting 60 pg (0.55 pmol) of DMS and 150 pg (0.80 pmol) DMDS. Sensitivity was 10–50 times greater with DMS and 4–20 times greater with DMDS when the photoionization detector was employed than when the flame ionization detector was employed.


Detection And Characterization Of Amitraz Resistance In The Southern Cattle Tick, Boophilus Microplus (Acari: Ixodidae), Andrew Y. Li, Ronald B. Davey, Robert J. Miller, John E. George Jan 2004

Detection And Characterization Of Amitraz Resistance In The Southern Cattle Tick, Boophilus Microplus (Acari: Ixodidae), Andrew Y. Li, Ronald B. Davey, Robert J. Miller, John E. George

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Amitraz, a formamidine acaricide, plays an important role in the control of the southern cattle tick, Boophilus microplus (Canestrini), and other tick species that infest cattle, dogs, and wild animals. Although resistance to amitraz in B. microplus was previously reported in several countries, the actual measurement of the level of amitraz resistance in ticks has been difficult to determine due to the lack of a proper bioassay technique. We conducted a survey, by using a newly reported technique that was a modification of the standard Food and Agriculture Organization larval packet test, to measure the levels of resistance to amitraz …


Seed Quality Qtl In A Prominent Soybean Population, D. L. Hyten Jr., V. R. Pantalone, C. E. Sams, A. M. Saxon, D. Landau-Ellis, T. R. Stefaniak, M. E. Schmidt Jan 2004

Seed Quality Qtl In A Prominent Soybean Population, D. L. Hyten Jr., V. R. Pantalone, C. E. Sams, A. M. Saxon, D. Landau-Ellis, T. R. Stefaniak, M. E. Schmidt

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is a versatile crop due to its multitude of uses as a high protein meal and vegetable oil. Soybean seed traits such as seed protein and oil concentration and seed size are important quantitative traits. The objective of this study was to identify representative protein, oil, and seed size quantitative trait loci (QTL) in soybean. A recombinant inbred line (RIL) population consisting of 131 F6-derived lines was created from two prominent ancestors of North American soy-beans (‘Essex’ and ‘Williams’) and the RILs were grown in six environments. One hundred simple sequence repeat (SSR) markers …


Long-Term Environmental Effects Of Conifer Removal To Achieve Aspen Release In Near-Stream Areas Within The Northern Sierras, Kenneth W. Tate, Bobette Jones, Melanie Mcfarland, Tom Rickman, Al Vasquez Jan 2004

Long-Term Environmental Effects Of Conifer Removal To Achieve Aspen Release In Near-Stream Areas Within The Northern Sierras, Kenneth W. Tate, Bobette Jones, Melanie Mcfarland, Tom Rickman, Al Vasquez

Aspen Bibliography

The advanced state of conifer encroachment in aspen stands in the Sierra Nevada and southern Cascades is retarding aspen regeneration, putting an estimated 70 plus percent of stands at immediate risk of extinction with a significant percentage of stands already lost.


Executive Summary The Role Of Ungulate Herbivory And Management On Ecosystem Patterns And Processes: A Problem Analysis, John G. Kie, Martin Vavra Jan 2004

Executive Summary The Role Of Ungulate Herbivory And Management On Ecosystem Patterns And Processes: A Problem Analysis, John G. Kie, Martin Vavra

Aspen Bibliography

No abstract provided.


Vascular Plant Species Inventory Of Richardson Bottoms Wildlife Viewing Area, Travis D. Marsico Jan 2004

Vascular Plant Species Inventory Of Richardson Bottoms Wildlife Viewing Area, Travis D. Marsico

Journal of the Arkansas Academy of Science

No abstract provided.


Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 5 2004, Several Authors Jan 2004

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 5 2004, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Molecular Differentiation Of Two Sibling Species Of The Black Fly Simulium Vittatum (Diptera: Simuliidae) Based On Random Amplified Polymorphic Dna, G. A. Duncan, P. H. Adler, K. P. Pruess, Thomas O. Powers Jan 2004

Molecular Differentiation Of Two Sibling Species Of The Black Fly Simulium Vittatum (Diptera: Simuliidae) Based On Random Amplified Polymorphic Dna, G. A. Duncan, P. H. Adler, K. P. Pruess, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33P, and run on polyacrylamide gels. The entire data set of 96 amplicons produced incomplete separation of the two cytospecies when subjected to neighbor-joining and maximum parsimony analyses. However, when analyzed within geographical regions, separate species status was supported. Boot- strap support for distinctness of the two cytospecies was best in Colorado where they were collected in true sympatry. The …


Glycosyltransferases Encoded By Viruses, Nicolas Markine-Goriaynoff, Laurent Gillet, James L. Van Etten, Haralambos Korres, Naresh Verma, Alain Vanderplasschen Jan 2004

Glycosyltransferases Encoded By Viruses, Nicolas Markine-Goriaynoff, Laurent Gillet, James L. Van Etten, Haralambos Korres, Naresh Verma, Alain Vanderplasschen

James Van Etten Publications

Studies of cellular biology in recent decades have highlighted the crucial roles of glycans in numerous important biological processes, raising the concept of glycomics that is now considered as important as genomics, transcriptomics and proteomics. For millions of years, viruses have been co-evolving with their hosts. Consequently, during this co-evolution process, viruses have acquired mechanisms to mimic, hijack or sabotage host processes that favour their replication, including mechanisms to modify the glycome. The importance of the glycome in the regulation of host–virus interactions has recently led to a new concept called ‘glycovirology’. One fascinating aspect of glycovirology is the study …


Morphological And Molecular Characterization Of Meloidogyne Mayaguensis Isolates From Florida, J. Brito, Thomas O. Powers, P. G. Mullin, Florida Department Of Agriculture And Consumer Services N., D. W. Dickson Jan 2004

Morphological And Molecular Characterization Of Meloidogyne Mayaguensis Isolates From Florida, J. Brito, Thomas O. Powers, P. G. Mullin, Florida Department Of Agriculture And Consumer Services N., D. W. Dickson

Department of Plant Pathology: Faculty Publications

The discovery of Meloidogyne mayaguensis is confirmed in Florida; this is the first report for the continental United States. Meloidogyne mayaguensis is a virulent species that can reproduce on host cultivars bred for nematode resistance. The perineal patterns of M. mayaguensis isolates from Florida show morphological variability and often are similar to M. incognita. Useful morphological characters for the separation of M. mayaguensis from M. incognita from Florida are the male stylet length values (smaller for M. mayaguensis than M. incognita) and J2 tail length values (greater for M. mayaguensis than M. incognita). Meloidogyne mayaguensis values for …


The Hopptof Locus Of Pseudomonas Syringae Pv. Tomato Dc3000 Encodes A Type Iii Chaperone And A Cognate Effector, Libo Shan, Hye-Sook Oh, Jianfu Chen, Ming Guo, Jianmin Zhou, James R. Alfano, Alan Collmer, Xu Jia, Xiaoyan Tang Jan 2004

The Hopptof Locus Of Pseudomonas Syringae Pv. Tomato Dc3000 Encodes A Type Iii Chaperone And A Cognate Effector, Libo Shan, Hye-Sook Oh, Jianfu Chen, Ming Guo, Jianmin Zhou, James R. Alfano, Alan Collmer, Xu Jia, Xiaoyan Tang

Department of Plant Pathology: Faculty Publications

Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcFPto and HopFPto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcFPto and HopFPto encode a type …


Effects Of Choline, Betaine, And Wheat Floral Extracts On Growth Of Fusarium Graminearum, Jessica S. Engle, Patrick E. Lipps, Terry L. Graham, Michael J. Boehm Jan 2004

Effects Of Choline, Betaine, And Wheat Floral Extracts On Growth Of Fusarium Graminearum, Jessica S. Engle, Patrick E. Lipps, Terry L. Graham, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Fusarium head blight has been more severe when infection occurs during anthesis, indicating that floral organs may be important infection courts. Choline acetate and glycinebetaine have been extracted from wheat and reported to be growth stimulants of Fusarium graminearum. They are hypothesized to enhance infection and tissue colonization. Growth of F. graminearum was examined on media amended with extracts from floral parts of nine wheat genotypes with various Fusarium head blight resistance levels. Results indicated no significant effect of anther, palea, or lemma extracts on radial growth when compared with unamended controls. Effects on spore germination and hyphal growth …


Nematode Molecular Diagnostics: From Bands To Barcodes, Tom Powers Jan 2004

Nematode Molecular Diagnostics: From Bands To Barcodes, Tom Powers

Department of Plant Pathology: Faculty Publications

Nematodes are considered among the most difficult animals to identify. DNA-based diagnostic methods have already gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Researchers are currently in an information-gathering mode, with intensive efforts applied to accumulating nucleotide sequence of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes. Important linkages with collateral data such as digitized images, video clips and specimen voucher web pages are being established on GenBank and NemATOL, the nematode-specific Tree of Life database. The growing DNA taxonomy of nematodes has lead to their use in testing specific short sequences …


Type Iii Secretion System Effector Proteins: Double Agents In Bacterial Disease And Plant Defense, James R. Alfano, Alan Collmer Jan 2004

Type Iii Secretion System Effector Proteins: Double Agents In Bacterial Disease And Plant Defense, James R. Alfano, Alan Collmer

Department of Plant Pathology: Faculty Publications

Many phytopathogenic bacteria inject virulence effector proteins into plant cells via a Hrp type III secretion system (TTSS). Without the TTSS, these pathogens cannot defeat basal defenses, grow in plants, produce disease lesions in hosts, or elicit the hypersensitive response (HR) in nonhosts. Pathogen genome projects employing bioinformatic methods to identify TTSS Hrp regulon promoters and TTSS pathway targeting signals suggest that phytopathogenic Pseudomonas, Xanthomonas, and Ralstonia spp. harbor large arsenals of effectors. The Hrp TTSS employs cus- tomized cytoplasmic chaperones, conserved export components in the bacterial en- velope (also used by the TTSS of animal pathogens), and …


Effect Of Light Duration On Pulvinar Motor Organ Movements In Phaseolus Vulgaris, Thomas William Rinehart Jan 2004

Effect Of Light Duration On Pulvinar Motor Organ Movements In Phaseolus Vulgaris, Thomas William Rinehart

Presidential Scholars Theses (1990 – 2006)

With a well established model in place, the main objective of this study will be to determine if the pulvinar motor organs have a threshold radiation level to elicit paraheliotropic movements. Plants will be illuminated with full spectrum light at varying intensities and periods. Observation will take place minute-by-minute in an effort to denote certain lighting conditions which might trigger paraheliotropism.


Chemical Defenses In Garlic Mustard (Alliaria Petiolata) And Their Potential Role In Species Interactions In Forest Understories, Don Cipollini Jan 2004

Chemical Defenses In Garlic Mustard (Alliaria Petiolata) And Their Potential Role In Species Interactions In Forest Understories, Don Cipollini

Biological Sciences Faculty Publications

Garlic mustard [Alliaria petiolata [(M. Bieb) Cavara & Grande; Brassicaceae] is a European native biennial herb, first recorded on Long Island, NY in the 1860s, and is expanding rapidly in northeastern and midwestern forests in the U.S. and in southern Canada. Garlic mustard flourishes in moist woodlands with moderate exposure to light, but it can grow in a diversity of other habitats. It is found in natural areas, woodlots, and along edges of agricultural fields and lawns throughout North America. Several life history traits likely contribute to the invasiveness of this species. It has a high inbreeding rate and can …


Pest Management Benefits Of Compost Mulch In Apple Orchards, M. W. Brown, Thomas Tworkoski Jan 2004

Pest Management Benefits Of Compost Mulch In Apple Orchards, M. W. Brown, Thomas Tworkoski

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effect of compost application on weed, fungal, and insect pest management in apple orchards was investigated from 1999 to 2001. Composted poultry manure was applied in June 1999 to half of two small research orchards which had previously received little or no management. The compost provided weed control for 1 year after application. There was no effect of compost on apple scab (Venturia inaequalis) infection. In a laboratory experiment, growth of the brown rot fungus (Monilinia fructicola) was significantly slower on a compost substrate than a sterilized compost substrate. The compost significantly affected arthropod abundance …


Conservation Of Natural Enemies In Cotton: Role Of Insect Growth Regulators In Management Of Bemisia Tabaci, Steven E. Naranjo, Peter C. Ellsworth, James R. Hagler Jan 2004

Conservation Of Natural Enemies In Cotton: Role Of Insect Growth Regulators In Management Of Bemisia Tabaci, Steven E. Naranjo, Peter C. Ellsworth, James R. Hagler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Field studies were conducted from 1997 to 1999 to contrast the effects of two insect growth regulators (IGRs) and conventional insecticides on natural enemy conservation in cotton within the context of alternative management strategies for Bemisia tabaci (Gennadius). Compared with an untreated control, insecticide regimes based on the initial use of the IGR buprofezin or pyriproxyfen reduced densities of eight predator taxa out of 20 examined in at least one year, including common species such as Geocoris punctipes (Say), Nabis alternatus Parshley, Chrysoperla carnea s.l., and the empidid fly Drapetis nr. divergens. Patterns of predator and pest population change relative …


Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2004, Agricultural Experiment Station, Plant Science Department College Of Agriculture And Biological Sciences Jan 2004

Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2004, Agricultural Experiment Station, Plant Science Department College Of Agriculture And Biological Sciences

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2004 progress report for the Central Crops and Soils Research Station at Highmore, South Dakota State College. This document represents the research conducted at the Station during the 2004 crop season including: temperature and precipitation data, field evaluations of woody plant materials, optimal management of drought-tolerant legumes and warm season annual grasses, winter wheat breeding, oat research, weed control fertilizer and soil test effects, aphid infestations, barley yellow dwarf Incidence, plant growth and yield of winter wheat in relation to planting date and seed treatment, small grain variety performance trials, corn breeding, and an evaluation of native …


Windbreaks In North American Agricultural Systems, James R. Brandle, Laurie Hodges, Xinhua H. Zhou Jan 2004

Windbreaks In North American Agricultural Systems, James R. Brandle, Laurie Hodges, Xinhua H. Zhou

Department of Agronomy and Horticulture: Faculty Publications

Windbreaks are a major component of successful agricultural systems throughout the world. The focus of this chapter is on temperate-zone, commercial, agricultural systems in North America, where windbreaks contribute to both producer profitability and environmental quality by increasing crop production while simultaneously reducing the level of off-farm inputs. They help control erosion and blowing snow, improve animal health and survival under winter conditions, reduce energy consumption of the farmstead unit, and enhance habitat diversity, providing refuges for predatory birds and insects. On a larger landscape scale windbreaks provide habitat for various types of wildlife and have the potential to contribute …


The High Mountain Vegetation Of Turkey - A State Of The Art Report, Including A First Annotated Conspectus Of The Major Syntaxa, Gerald Parolly Jan 2004

The High Mountain Vegetation Of Turkey - A State Of The Art Report, Including A First Annotated Conspectus Of The Major Syntaxa, Gerald Parolly

Turkish Journal of Botany

This contribution spotlights the present research on the (oreal) subalpine to subnival mountain vegetation of Turkey. It concisely compiles our present knowledge, which is very heterogeneous in geographical terms. The situation is comparatively good in NW and W Anatolia and the western half of the Taurus range, from where a first consolidation stage can be reported. By contrast, the E Taurus remains a largely unexplored area. The Pontic chains have hitherto attracted a few local researchers only, and those important pioneering accounts cover less than half of the vegetation types actually present. The results of the last years' field work …


Studies On The History Of Botany In Turkey, K. Hüsnü Can Başer Jan 2004

Studies On The History Of Botany In Turkey, K. Hüsnü Can Başer

Turkish Journal of Botany

No abstract provided.


Census Catalogue Of Polish Mosses (2003), Adnan Erdağ Jan 2004

Census Catalogue Of Polish Mosses (2003), Adnan Erdağ

Turkish Journal of Botany

No abstract provided.


Influence Of Some Plant Extracts And Microbioagents On Some Physiological Traits Of Faba Bean Infected With Botrytis Fabae, Yehia A.G. Mahmoud, Mohsen Ebrahim, Magda M. Aly Jan 2004

Influence Of Some Plant Extracts And Microbioagents On Some Physiological Traits Of Faba Bean Infected With Botrytis Fabae, Yehia A.G. Mahmoud, Mohsen Ebrahim, Magda M. Aly

Turkish Journal of Botany

Laboratory and greenhouse experiments were conducted to assess the efficacy of Eucalyptus citriodora Hook., Ipomoea carnea Jacq., Cuminum cyminum L., Allium sativum L. and Hyoscyamus muticus L. leaf extracts, and of Streptomyces exfoliatus (Waksman & Curtis) Waksman & Henrici (S) and Trichoderma harzianum Rifai (T) in controlling Botrytis fabae, which causes chocolate spot disease in the faba bean. Laboratory studies supported the use of leaf extracts of E. citriodora (Ex. 1) and I. carnea (Ex. 2) in preference to other extracts for controlling the mycelial growth of B. fabae. In addition, the mixture S + T was the best of …


Effects Of Burial And Soil Condition On Postharvest Mortality Of Boll Weevils (Coleoptera: Curculionidae) In Fallen Cotton Fruit, S. M. Greenberg, A. T. Showler, T. W. Sappington, J. M. Bradford Jan 2004

Effects Of Burial And Soil Condition On Postharvest Mortality Of Boll Weevils (Coleoptera: Curculionidae) In Fallen Cotton Fruit, S. M. Greenberg, A. T. Showler, T. W. Sappington, J. M. Bradford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Effects of soil condition and burial on boll weevil, Anthonomus grandis grandis Boheman, mortality in fallen cotton, Gossypium hirsutum L., fruit were assessed in this study. During hot weather immediately after summer harvest operations in the Lower Rio Grande Valley of Texas, burial of infested fruit in conventionally tilled field plots permitted significantly greater survival of weevils than in no-tillage plots. Burial of infested squares protected developing weevils from heat and desiccation that cause high mortality on the soil surface during and after harvest in midsummer and late summer. A laboratory assay showed that burial of infested squares resulted in …


Small Potassium Ion Channel Proteins Encoded By Chlorella Viruses, Ming Kang, Anna Moroni, Sabrina Gazzarrini, Dario Difrancesco, Gerhard Thiel, Maria Severino, James L. Van Etten Jan 2004

Small Potassium Ion Channel Proteins Encoded By Chlorella Viruses, Ming Kang, Anna Moroni, Sabrina Gazzarrini, Dario Difrancesco, Gerhard Thiel, Maria Severino, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Kcv, a 94-aa protein encoded by Paramecium bursaria chlorella virus 1, is the smallest known protein to form a functional potassium ion channel and basically corresponds to the ‘‘pore module’’ of potassium channels. Both viral replication and channel activity are inhibited by the ion channel blockers barium and amantadine but not by cesium. Genes encoding Kcv-like proteins were isolated from 40 additional chlorella viruses. Differences in 16 of the 94 amino acids were detected, producing six Kcv-like proteins with amino acid substitutions occurring in most of the functional domains of the protein (N terminus, transmembrane 1, pore helix, selectivity filter, …