Digital Commons Network^™

Wheat Cultivar-Specific Selection Of 2,4-Diacetylphloroglucinol-Producing Fluorescent Pseudomonas Species From Resident Soil Populations, M. Mazzola, Deanna L. Funnell, J.M. Raaijmakers

Department of Plant Pathology: Faculty Publications

An emerging body of evidence indicates a role for plant genotype as a determinant of the species and genetic composition of the saprophytic microbial community resident to the rhizosphere. In this study, experiments were conducted to determine the capacity of five different wheat cultivars to enhance resident populations and support introduced strains of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent pseudomonads, a group of bacteria known to provide biological control of several soilborne diseases. When soils were cropped with three successive 28-day growth cycles of wheat, the 2,4- DAPG-producing strains were consistently recovered from the rhizosphere of the cultivar Lewjain, and commonly were present …

Spinach Curly Top Virus: A Newly Described Curtovirus Species From Southwest Texas With Incongruent Gene Phylogenies, Surendranath Baliji, Mark C. Black, Roy C. French, Drake C. Stenger, Garry Sunter

Department of Plant Pathology: Faculty Publications

A curtovirus associated with a disease of spinach was isolated in southwest Texas during 1996. Disease symptoms included severe stunting and chlorosis, with younger leaves curled, distorted, and dwarfed. Viral DNA was purified and an infectious clone obtained. Agroinoculation using a construct bearing full-length tandem repeats of the cloned viral genome resulted in systemic infection of species in six of seven plant families tested, indicating that the virus has a wide host range. Symptoms produced in spinach agroinoculated with cloned viral DNA were similar to those observed in the field. Viral single-stranded and double-stranded DNA forms typical of curtovirus infection …

Promoters From Chlorella Virus Genes Providing For Expression Of Genes In Prokaryotic And Eukaryotic Hosts, Amitava Mitra, James L. Van Etten

Department of Plant Pathology: Faculty Publications

The invention is directed to novel promoters or mutants thereof from Chlorella virus DNA methyltansferase genes. A Chlorella Virus gene promoter is operably linked to a first and/or Second DNA sequence encoding a gene that is different from the Chlorella virus to form an expression cassette. An expression cassette can be introduced into prokaryotic and/or eukaryotic cells and can provide for a high level of expression of the gene encoded by the first and/or Second DNA sequence. The invention also provides a method for Screening other Chlorella virus genes for promoters that can function to express a heterologous gene in …

A New Integrated Genetic Linkage Map Of The Soybean, Q.J. Song, L.F. Marek, R.C. Shoemaker, K.G. Lark, V.C. Concibido, X. Delannay, James E. Specht, P.B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

A total of 391 simple sequence repeat (SSR) markers designed from genomic DNA libraries, 24 derived from existing GenBank genes or ESTs, and five derived from bacterial artificial chromosome (BAC) end sequences were developed. In contrast to SSRs derived from EST sequences, those derived from genomic libraries were a superior source of polymorphic markers, given that the mean number of tandem repeats in the former was significantly less than that of the latter (P<0.01). The 420 newly developed SSRs were mapped in one or more of five soybean mapping populations: ‘Minsoy’ × ‘Noir 1’, ‘Minsoy’ × ‘Archer’, ‘Archer’ × ‘Noir 1’, ‘Clark’ × ‘Harosoy’, and A81-356022 × PI468916. The JoinMap software package was used to combine the five maps into an integrated genetic map spanning 2,523.6 cM of Kosambi map distance across 20 linkage groups that contained 1,849 markers, including 1,015 SSRs, 709 RFLPs, 73 RAPDs, 24 classical traits, six AFLPs, ten isozymes, and 12 others. The number of new SSR markers added to each linkage group ranged from 12 to 29. In the integrated map, the ratio of SSR marker number to linkage group map distance did not differ among 18 of the 20 linkage groups; however, the SSRs were not uniformly spaced over a linkage group, clusters of SSRs with very limited recombination were frequently present. These clusters of SSRs may be indicative of gene-rich regions of soybean, as has been suggested by a number of recent studies, indicating the significant association of genes and SSRs. Development of SSR markers from map-referenced BAC clones was a very effective means of targeting markers to marker-scarce positions in the genome.

The Pseudomonas Syringae Hopptov Protein Is Secreted In Culture And Translocated Into Plant Cells Via The Type Iii Protein Secretion System In A Manner Dependent On The Shcv Type Iii Chaperone, Misty D. Wehling, Ming Guo, Zheng Qing Fu, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial plant pathogen Pseudomonas syringae depends on a type III protein secretion system and the effector proteins that it translocates into plant cells to cause disease and to elicit the defense-associated hypersensitive response on resistant plants. The availability of the P. syringae pv. tomato DC3000 genome sequence has resulted in the identification of many novel effectors. We identified the hopPtoV effector gene on the basis of its location next to a candidate type III chaperone (TTC) gene, shcV, and within a pathogenicity island in the DC3000 chromosome. A DC3000 mutant lacking ShcV was unable to secrete detectable amounts …

Effects Of Management Practicesmon Grassland Birds: Brewer’S Sparrow, Brett Walker

United States Geological Survey: Effects of Management Practices on Grassland Birds

Information on the habitat requirements and effects of habitat management on grassland birds were summarized from information in more than 5,500 published and unpublished papers. A range map is provided to indicate the relative densities of the species in North America, based on Breeding Bird Survey (BBS) data. Although birds frequently are observed outside the breeding range indicated, the maps are intended to show areas where managers might concentrate their attention. It may be ineffectual to manage habitat at a site for a species that rarely occurs in an area. The species account begins with a brief capsule statement, which …

Forage News [2004-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Field Day
• AFGC to Meet In Roanoke, Virginia June 12-16
• Forage Species and Spatial Effects on the Dietary Intake of Goats
• Kentucky Ranks High in Beef
• Forage-Animal Production Research Unit: Most Recent Agriculture Research Service Location
• Calibrating Seeders
• Upcoming Events

The Prairie Naturalist Volume 36, No.2 June 2004

The Prairie Naturalist

PREDICTING NEONATAL AGE OF WHITE-TAILED DEER IN THE NORTHERN GREAT PLAINS . . T. J. Brinkman, K. L. Monteith, J. A. Jenks, and C. S. DePemo

DUCK NESTING ON ROTATIONAL AND CONTINUOUS GRAZED PASTURES IN NORTH DAKOTA . R. K. Murphy, D. J. Schindler, and R. D. Crawford

MORPHOLOGY OF LESSER PRAIRIE-CHICKENS IN KANSAS ▪ C. A. Hagen, J. C. Pitman, R. J. Robel, and R. D. Applegate

DAILY AND SEASONAL BEHAVIOR OF BISON ON AN OKLAHOMA TALLGRASS PRAIRIE . E. J. Maichak, K. L. Schuler, and M. E. Payton

CHRISTMAS BIRD COUNTS FOR NORTH DAKOTA 2003 ▪ R. N. …

Review Of Bacterial Disease Resistance In Plants: Molecular Biology And Biotechnological Applications By P. Vidhyasekaran, Marty Dickman

Department of Plant Pathology: Faculty Publications

The molecular biology of plant-bacterial interactions is a field that continues to provide a wealth of information in the context of infectious diseases of plants. Thus, it is curious that few textbooks on this topic are available. Admittedly, active areas of research change rapidly, and book content can be rendered obsolete prior to publication. Even given this scenario, a textbook that summarizes the development (both conceptually and experimentally) of research strategies, the status of model systems, and future issues is needed. Thus, the timing and need for this broad-based volume, which covers the molecular phytobacteriology, is long overdue. An important …

Organic Horticulture: Strategic Opportunities In Western Australia, Steven Mccoy

Bulletins 4000 -

This report considers the potential to develop organic horticulture in WA. The study examines key factors limiting industry scale and scope and identifies preferred soil types, regional locations, prospective crops and potential scale where organic production may offer comparative economic advantage.

Multi-Trophic Level Interactions In A Cassava-Maize Mixed Cropping System In The Humid Tropics Of West Africa, F. Schulthess, A. Chabi-Olaye, S. Gounou

All Peer-Reviewed Publications

Multi-trophic level interactions in a mixed crop, involving cassava and maize, were studied in derived-savanna in Benin, West Africa. Two trials were planted, one during the short rainy season two months before onset of the dry season and one during the long rainy season in spring. Key pests under study on maize were the noctuid Sesamia calamistis Hampson and the pyralids Eldana saccharina Walker and Mussidia nigrivenella Ragonot, and on cassava, the exotic mealybug, Phenacoccus manihoti Matile-Ferrero and its encyrtid parasitoid Apoanagyrus lopezi De Santis. Both crops received insecticide treatments to assess the crop loss by a pest species. On …

K8 And K12 Are Biotinylated In Human Histone H4, Gabriela Camporeale, Elizabeth E. Shubert, Gautam Sarath, Ronald Cerny, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Folding of DNA into chromatin is mediated by binding to histones such as H4; association of DNA with histones is regulated by covalent histone modifications, e.g. acetylation, methylation, and biotinylation.We sought to identify aminoacid residues that are biotinylated in histone H4,and to determine whether acetylation and methylation of histones affect biotinylation. Synthetic peptides spanning fragments of human histone H4 were biotinylated enzymatically using biotinidase. Peptide-bound biotin was probed with streptavidin– peroxidase. Peptides based on the N-terminal sequence of histone H4 were effectively recognized by biotinidase as substrates for biotinylation; in contrast, peptides based on the C-terminal sequences were not biotinylated. …

Tyrosine B10 Inhibits Stabilization Of Bound Carbon Monoxide And Oxygen In Soybean Leghemoglobin, Suman Kundu, George C. Blouin, Scott A. Premer, Gautam Sarath, John S. Olson, Mark S. Hargrove

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Detailed comparisons of the carbon monoxide FTIR spectra and ligand-binding properties of a library of E7, E11, and B10 mutants indicate significant differences in the role of electrostatic interactions in the distal pockets of wild-type sperm whale myoglobin and soybean leghemoglobin. In myoglobin, strong hydrogen bonds from several closely related conformations of the distal histidine (His^E7) side chain preferentially stabilize bound oxygen. In leghemoglobin, the imidazole side chain of His^E7 is confined to a single conformation, which only weakly hydrogen bonds to bound ligands. The phenol side chain of TyrB10 appears to “fix” the position of His …

Inhibitor Of Plant Peptide Deformylase For Use As Broad-Spectrum Herbicides And Method For Identifying The Same, Robert L. Houtz, Lynnette M. A. Dirk, Mark Allan Williams

Horticulture Faculty Patents

The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides.

Plant Trichome Gland Specific Promoter Sequence, George J. Wagner, Sushen Gan, Erming Wang, Rui Wang

Kentucky Tobacco Research and Development Center Faculty Patents

The present invention relates to a trichome specific regulatory sequence.

Does Seed Size Affect The Rate Of Germination And Early Seedling Growth In Hairy Vetch?, Joy Ekpo

McCabe Thesis Collection

Size is an important characteristic of seed quality because larger seeds with larger volume contain more resources and are likely to exhibit greater vigor than smaller seeds (Ellis, 1992). In a range of plant species, seed size has been shown to affect germination rate, emergence rate, success of establishment, and growth (Black 1957; Bentley et al. 1980; Sanderson et al. 2002). Hairy vetch is an annual legume that is used for improvement of soil for pasture and to provide ground cover protecting against erosion (Duke 1981; Undersander 2002; Sattell et al 1988). Problems facing the establishment of hairy vetch are …

Moisture Potential Affects Rate Of Leaf Appearance In Cool Season Grasses, Nina Cherone Terrell

McCabe Thesis Collection

The rate of leaf appearance and onset of growth in cool season grasses may be influenced by soil moisture potential and exposure to below freezing temperatures. Levels of moisture potential and days exposed to freezing temperatures, which will restrict leaf appearance, are not well defined. The first part of this study was to determine the effects of different moisture potentials on the leaf appearance rates in two cool season grasses. Italian ryegrass (IRG) and tall wheat grass (TWG) were grown hydroponically at 4 moisture potentials in seed pouches in an incubator under a photoperiod of 13 hours light and 11 …

Center For Grassland Studies Newsletter, Spring 2004, Volume 10, No. 2

Center for Grassland Studies: Newsletters

CGS Coordinates New Professional Golf Management Program

Alternatives to Grazing Drought-Stricken Pastures by Ivan Rush, Panhandle Research and Extension Center, UNL

2004 Nebraska Grazing Conference in Kearney August 10-11

Dave and Loretta Hamilton: Grazing and Ranch Management Mentors by Dana Larsen, Natural Resources Conservation Service, USDA

Loess Hills Conservation Measures Improve Rangeland Conditions and Aid American Burying Beetle in Nebraska by Thomas Walker, Jr., Nebraska Game and Parks Commission Jerry Volesky, Department of Agronomy and Horticulture, UNL Wyatt Hoback, Department of Biology, University of Nebraska at Kearney

XX International Grassland Congress, Ireland, 2005

Allan Savory on Holistic Resource Management at …

Influence Of Poultry Litter On Dark Tobacco Growth And Soil Nutrient Availability, Jason Simmons

Masters Theses & Specialist Projects

Due to the relatively high value of dark tobacco compared with fertilizer costs, nitrogen is recommended at levels as high as 338 kg ha"1. Such rates of inorganic fertilizers increase the osmotic pressure of the soil solution and soil acidity, often causing reduced stands, Mn toxicity, Mo and Ca deficiencies, and reduced yield and quality of the cured leaf (Sims et al., 1984). Poultry litter utilized as a nutrient source is an inexpensive alternative to this dilemma due to its relatively neutral or alkaline composition. However, due the amount of chloride present in poultry litter, the University of Kentucky advises …

National Science Foundation-Sponsored Workshop Report. Draft Plan For Soybean Genomics, Gary Stacey, Lila N Vodkin, Wayne A. Parrott, Randy C. Shoemaker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Recent efforts to coordinate and define a research strategy for soybean (Glycine max) genomics began with the establishment of a Soybean Genetics Executive Committee, which will serve as a communication focal point between the soybean research community and granting agencies. Secondly, a workshop was held to define a strategy to incorporate existing tools into a framework for advancing soybean genomics research. This workshop identified and ranked research priorities essential to making more informed decisions as to how to proceed with large scale sequencing and other genomics efforts. Most critical among these was the need to finalize a physical …

The Influence Of Environmental Temperature And Substrate Initial Moisture Content On Aspergillus Niger Growth And Phytase Production In Solid−State Cultivation, Seaborn B. Carter, Sue E. Nokes, Czarena L. Crofcheck

Biosystems and Agricultural Engineering Faculty Publications

Aspergillus niger is being used commercially for phytase production utilizing solid-state cultivation; however, no studies have been published that investigated the optimal environmental temperature and initial substrate water content to maximize fungal growth and/or phytase production. Solid-state cultivations of Aspergillus niger on wheat bran and soybean meal were conducted at three temperatures (25°C, 30°C, and 35°C) and three initial moisture contents (50%, 55%, and 60% wet basis) in a split-plot full-factorial experimental design. Fermentations were conducted for 0, 24, 48, 72, and 120 h. The containers were sampled destructively and assayed for phytase activity and glucosamine concentration as an estimate …

A Study Of Invasive Species Cover Near Roads In A Red Rock Canyon Blackbrush Community, Christopher Keough

UNLV Theses, Dissertations, Professional Papers, and Capstones

The monitoring of Mojave Desert plant communities during and following disturbance is an important process that could provide invaluable information about disturbance/recovery regimes in similar arid environments across the globe. Blackbrush communities are of particular interest because of their low replacement rate, which makes them highly susceptible to disturbance. Roads in the Mojave Desert have been associated with soil compaction resulting in a lack of vegetation as well as an increase in invasive species cover in the immediate proximity. To investigate these statements, eight fifty-foot line transects were established in each of three plot types (perpendicular to dirt roads, perpendicular …

Cattle As Grazing Management And Seed Dispersal Tools For Increasing Native Species Diversity On Great Basin Rangelands, Marina K. Whitacre

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A series of experiments evaluated: 1) the influence of seed intake and gut retention time on seed passage, recovery, and germinability; 2) fecal seeding and broadcast /trampling as techniques to incorporate seeds into a well-established Agropyron desertorum (Fisch.) Schult. stand in Skull Valley , Utah; 3) intensive grazing as a means to reduce Agropyron biomass and increase establishment and survival of seeded species; and 4) the recovery and germinability of seed extracted from dung collected from the field. Two shrubs (Artemisia tridentata Nutt. ssp. wyomingensis Beetle & Young and Atriplex confertifolia Torr. & Frem.), a grass (Elymus elymoides (Raf.) …

Quantifying Losses Of Understory Forage In Aspen Stands On The Dixie And Fishlake National Forests, Barton R. Stam

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The West has lost up to 60% of its historic aspen stands over the last century, probably as a result of the successional tendency of aspen to be replaced by coniferous species in the absence of periodic fires. One of several major impacts of this change is the loss of understory forage as conifer canopy cover increases. I measured understory biomass in aspen stands ranging from 0% to 81 % absolute conifer cover in the canopy and found that understory production declines exponentially as conifers replace aspen. I also did an economic analysis to determine the value of the forage …

An Evaluation Of Submersed Aquatic Vegetation Transplanting Methods For Lake Pontchartrain Restoration, Donald Anthony Rodrigue Jr.

LSU New Orleans Theses and Dissertations

The general goals of this study were to evaluate transplant methods for Lake Pontchartrain submersed aquatic vegetation (SAV) restoration, and to compare survival and expansion of transplanted SAV among four sites. Two experiments were conducted from summer 2000 through spring 2001 at two north shore and two south shore sites in Lake Pontchartrain. Experiment 1 involved testing the effects of transplanting with matting, staples, exclosures, and interspecific interaction on survival and expansion of Ruppia maritima and Vallisneria americana transplants in Lake Pontchartrain. Experiment 2 involved testing the effects of transplanting with matting, staples, and plugs on Ruppia survival and expansion. …

Feasibility Of Site-Specific Management Of Corn Hybrids And Plant Densities In The Great Plains, J.F. Shanahan, Thomas A. Doerge, Jerry J. Johnson, Merle F. Vigil

Department of Agronomy and Horticulture: Faculty Publications

The goal of this research was to determine the potential for use of site-specific management of corn hybrids and plant densities in dryland landscapes of the Great Plains by determining (1) within-field yield variation, (2) yield response of different hybrids and plant densities to variability, and (3) landscape attributes associated with yield variation. This work was conducted on three adjacent fields in eastern Colorado during the 1997, -98, and -99 seasons. Treatments consisted of a combination of two hybrids (early and late maturity) and four plant densities (24,692, 37,037, 49,382 and 61,727 plants ha^-1) seeded in replicated long …

Evaluation Of The Escherichia Coli Threonine Deaminase Gene As A Selectable Marker For Plant Transformation, A. Ebmeier, L. Allison, Heriberto D. Cerutti, Thomas E. Clemente

Center for Plant Science Innovation: Faculty Publications

The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to α-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. Previous reports have shown that in planta expression of the wild-type Escherichia coli TD, ilvA, or an Ile-insensitive mutant designated ilvA-466, increased cellular concentrations of Ile. A structural analog of Ile, L-O-methylthreonine (OMT), is able to compete effectively with Ile during translation and induce cell death. It has been postulated that OMT …

Forage News [2004-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Spring Grazing School – Final Call
• Forage Extension Website
• Dr. Mike Collins Accepts Position at Mississippi State University
• Mr. Allen Johnson Accepts Position with Montgomery County Regional Marketing Association
• Hay Contest Winners
• Rotational Grazing’s Efficient
• Emergence and Survival of Legumes Seeded into Pastures
• Does Fertilizer Harm Soil Microbes?
• Kentucky Farm Numbers
• Upcoming Events

Defining Soil Type And Habitat Characteristics Of The Arctomecon Californica, Philip S. Childers

UNLV Theses, Dissertations, Professional Papers, and Capstones

A. californica can be considered a rare endemic species, which is believed to be restricted by unique soil relationships. These relationships make the species vulnerable to anthropogenic habitat disturbance. Although A. californica is listed as critically endangered by the state of Nevada, further research is needed before the species can be listed as federally endangered or threatened. This study used primary observational data and secondary GIS compatible data to characterize A. californica habitat. Representative sampling techniques were used to select observations from derived soil types. Although a majority of A. californica populations were found to occur in gypsic soil types, …

Seed Bank Response To Fire And Successional Trajectory Of A Virginia Small Depression Pocosin, Jay F. Bolin

Biological Sciences Theses & Dissertations

Pocosins are depressional wetlands maintained by fire. Plants in fire adapted systems can use a variety of mechanisms for survival and recruitment. I examined the seed bank response to fire (500 to 700 °C) and wet heat treatments (50 and 75 °C) in a Virginia small depression pocosin. I quantified seedling abundance, richness, and Sphagnum density. Extant vegetation was sampled with a nested plot design at each seed bank sampling area. The seed flora was compared with the extant vegetation at three size classes with Sorenson similarity values. Data from the fire treatment indicates that a simulated drought burn may …