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Letter From The Dean, Lalit Verma Jan 2009

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

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Variation Of Morphology And Elemental Concentrations In The California Nickel Hyperaccumulator Streptanthus Polygaloides (Brassicaceae), Robert S. Boyd, Michael A. Wall, Scott R. Santos, Michael A. Davis Jan 2009

Variation Of Morphology And Elemental Concentrations In The California Nickel Hyperaccumulator Streptanthus Polygaloides (Brassicaceae), Robert S. Boyd, Michael A. Wall, Scott R. Santos, Michael A. Davis

Faculty Publications

The Ni hyperaccumulator Strepthanthus polygaloides (Brassicaceae) is one of a handful of Ni hyperaccumulators known from continental North America. Surveys have revealed four distinctive morphs of this species, relying primarily on floral traits (sepal color and shape): a purple sepal morph (P), a yellow sepal morph (Y), a morph in which sepals start yellow and mature to purple (Y/P), and a morph with light yellow undulate sepals (U). In this study, we raised plants from ten populations (five Y, three P, one Y/P, and one U) under uniform greenhouse conditions to determine if morphs varied in morphology and elemental concentrations …

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A New Record For The Flora Of Turkey: Cirsium Candelabrum Griseb. (Cirsium Sect. Cirsium, Asteraceae, Cynareae), Bayram Yildiz, Tuncay Di̇rmenci̇, Turan Arabaci Jan 2009

A New Record For The Flora Of Turkey: Cirsium Candelabrum Griseb. (Cirsium Sect. Cirsium, Asteraceae, Cynareae), Bayram Yildiz, Tuncay Di̇rmenci̇, Turan Arabaci

Turkish Journal of Botany

Cirsium candelabrum Griseb. is reported for the first time from Thrace (Turkey). A description, including 2 photographs of the plant, and a map of its distribution in Turkey are presented.

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An Alien Species New To The Flora Of Turkey:Lysimachia Japonica Thunb. (Primulaceae), Sali̇h Terzi̇oğlu, Fergan Karaer Jan 2009

An Alien Species New To The Flora Of Turkey:Lysimachia Japonica Thunb. (Primulaceae), Sali̇h Terzi̇oğlu, Fergan Karaer

Turkish Journal of Botany

The Japanese species, Lysimachia japonica Thunb. (Primulaceae) has been recorded as a new alien for the Flora of Turkey from the NE Anatolia. The detailed description of the species together with its photographs is provided.

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Taxonomical And Chorological Notes On The Turkish Endemic Scorzonera Amasiana Hausskn. & Bornm. (Asteraceae), Muhi̇tti̇n Di̇nç, Yavuz Bağci Jan 2009

Taxonomical And Chorological Notes On The Turkish Endemic Scorzonera Amasiana Hausskn. & Bornm. (Asteraceae), Muhi̇tti̇n Di̇nç, Yavuz Bağci

Turkish Journal of Botany

Scorzonera amasiana Hausskn. & Bornm. is a stenoendemic species known from a few localities in Amasya Province. After the rediscovery of S. amasiana, it has been recorded from a new locality in Amasya. Using chorological and ecological data, morphological study of the collected specimens suggest that the recently described Scorzonera ekimii A.Duran is synonymous with S. amasiana. The conservation status of S. amasiana is also reviewed, based on the present distribution data.

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Macrofungi Of Göksun (Kahramanmaraş) District, Abdullah Kaya, Yusuf Uzun, İbrahi̇m Hali̇l Karacan Jan 2009

Macrofungi Of Göksun (Kahramanmaraş) District, Abdullah Kaya, Yusuf Uzun, İbrahi̇m Hali̇l Karacan

Turkish Journal of Botany

Macrofungi samples were collected from Göksun (Kahramanmaraş) district between 2005 and 2007. As a result of field and laboratory studies, 110 taxa belonging to 33 families and 70 genera in Ascomycetes and Basidiomycetes were identified. The distribution, habitat, and collection numbers of the identified species are given.

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Conservation Biology Of Asperula Daphneola (Rubiaceae) In Western Turkey, Sali̇h Gücel, Özcan Seçmen Jan 2009

Conservation Biology Of Asperula Daphneola (Rubiaceae) In Western Turkey, Sali̇h Gücel, Özcan Seçmen

Turkish Journal of Botany

A. daphneola O.Schwarz (Rubiaceae) was originally recorded from a single location on Nif Mountain (Kemalpaşa) in western Turkey. This study was carried out from 2001 to 2004 to investigate the causes of this endemic species^{'} restricted distribution. To that end, the environmental conditions and reproductive biology of the species were studied. As a result of field investigations, 4 additional locations were identified. These 5 localities (the peak of Nif Mountain, Alaca Mountain, Ayrıca Mountain, Bölme Hill, and another locality west of Nif Mountain) encompass an area of 2.5 km^2. The total number of individuals in these localities was estimated to …

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Dynamics Of 2,4-D In Generation Of Cytomorphological Variants In An Important Anticancerous And Antihepatotoxic Herb – Cichorium Intybus L., Zeba Khan, Mohammad Yunus Khalil Ansari, Honey Gupta, Sana Chaudhary Jan 2009

Dynamics Of 2,4-D In Generation Of Cytomorphological Variants In An Important Anticancerous And Antihepatotoxic Herb – Cichorium Intybus L., Zeba Khan, Mohammad Yunus Khalil Ansari, Honey Gupta, Sana Chaudhary

Turkish Journal of Botany

The genotoxic effect of 2,4-D was investigated in chicory (2n = 18). At 100 ppm concentration 4 variants differing in morphological traits were isolated and subjected to chromosomal analysis. Morphological variations in shape, height, and yield parameters were observed. Different cytological anomalies such as univalents, multivalents, bridges, laggards, chromosome stickiness, and polyads occurred in all 4 isolates. However, the frequency of these chromosomal irregularities decreased at anaphase, exhibiting recovery at later stages. Thus the above concentration of growth hormone 2,4-D can be effectively incorporated for raising viable mutants in this medicinally useful herb.

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The Effects Of Brassinosteroid On The Induction Of Biochemical Changes In Lycopersicon Esculentum Under Drought Stress, Mehri Behnamnia, Khosrow Manochehri Kalantari, Jalal Ziaie Jan 2009

The Effects Of Brassinosteroid On The Induction Of Biochemical Changes In Lycopersicon Esculentum Under Drought Stress, Mehri Behnamnia, Khosrow Manochehri Kalantari, Jalal Ziaie

Turkish Journal of Botany

Drought stress is considered a restricting factor for plant products; therefore many compounds were applied to minimise the harmful effects of stress. One type of these compounds that have antioxidative characteristics is brassinosteroids. In this experiment, when 4 fully expanded leaves of tomato plants appeared, 24-epibrassinolide (24-EBL) was sprayed onto the leaves at 0.01 and 1 µM concentrations for 3 days with a 1-day interval. Three levels of drought stress (0, 3, and 5 days withholding water) were applied. Thereafter, the effects of brassinosteroids and water stress were investigated on some biochemical and antioxidative parameters of tomato plants. Lipid peroxidation, …

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Gibberellic Acid Promotes Seed Germination In Penstemon Digitalis Cv. Husker Red, Anderson Machado De Mello, Nereu Augusto Streck, Erin E. Blankenship, Ellen Paparozzi Jan 2009

Gibberellic Acid Promotes Seed Germination In Penstemon Digitalis Cv. Husker Red, Anderson Machado De Mello, Nereu Augusto Streck, Erin E. Blankenship, Ellen Paparozzi

Department of Agronomy and Horticulture: Faculty Publications

Penstemon seed often shows an inconsistent or a low germination percentage. Although most select cultivars are propagated by cuttings, for export to other countries, seed is preferred. Three experiments were conducted to determine if soaking seed in gibberellic acid (GA3) would increase seed germination of Penstemon digitalis cv. Husker Red. GA3 concentrations used were 0, 10, 50, 100, 200, and 500 mg∙L–1 (first experiment); 0, 500, 1000, and 1500 mg∙L–1 (second experiment); and 0, 500, and 1000 mg∙L–1 (third experiment). The first and second experiments were conducted in a growth chamber, whereas the third …

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Evolution And Systematic Significance Of Reproductive Structures In The Genus Cuscuta (Dodders, Convolvulaceae): Pollen And Gynoecium, Mark Welsh Jan 2009

Evolution And Systematic Significance Of Reproductive Structures In The Genus Cuscuta (Dodders, Convolvulaceae): Pollen And Gynoecium, Mark Welsh

Theses and Dissertations (Comprehensive)

The genus Cuscuta (dodders, Convolvulaceae) is one of the most significant lineages of parasitic plants from economic, conservation, and anthropogenic perspectives. Members of the genus are twining stem parasites with no roots, lacking almost completely chlorophyll or its function, and gather required nutrition from their host via specialized haustorial connections. While there are almost 200 described species, problems with species identification exist because many diagnostic characters are restricted to their tiny flowers. Probably contributing to this identification difficulty is the fact that the group has not received taxonomic attention for over 75 years; even knowledge of its basic reproductive biology …

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Towards Reducing Fungicide Use In The Control Of Dollar Spot (Sclerotinia Homoeocarpa F.T. Bennett) Disease On Creeping Bentgrass (Agrostis Stolonifera L.), Kenneth Lee Cropper Jan 2009

Towards Reducing Fungicide Use In The Control Of Dollar Spot (Sclerotinia Homoeocarpa F.T. Bennett) Disease On Creeping Bentgrass (Agrostis Stolonifera L.), Kenneth Lee Cropper

University of Kentucky Master's Theses

Creeping bentgrass (Agrostis stolonifera L.) is commonly used on golf course greens and fairways in cool-humid regions but is plagued by numerous fungal diseases, one of which is dollar spot disease (Sclerotinia homoeocarpa F. T. Bennett). Dollar spot occurs frequently throughout the growing season requiring biweekly fungicide applications for complete control. The objective of this study was to investigate methods of reducing the number of fungicide applications needed to maintain dollar spot at acceptable levels through dew removal and potential mechanisms of resistance in bentgrass. In the first study, a combination of mowing three times a week and …

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Analysis Of The Arabidopsis Polyadenylation Factors Pap1, Cstf64 And Cstf77 And Their Characteristic Inter-Relationship, Amrita Bandyopadhyay Jan 2009

Analysis Of The Arabidopsis Polyadenylation Factors Pap1, Cstf64 And Cstf77 And Their Characteristic Inter-Relationship, Amrita Bandyopadhyay

University of Kentucky Master's Theses

3’-end modification by polyadenylation is a ubiquitous feature of almost all eukaryotic mRNA species and is catalyzed by a consortium of enzymes, the polyadenylation factors. Poly(A) polymerase (PAP), the enzyme catalyzing the addition of adenosine residues during the polyadenylation stage, exists in four isoforms within Arabidopsis. In silico and yeast two-hybrid studies showed that PAP1 has unique expression and interaction pattern in Arabidopsis, suggesting non-canonical functions of PAP1. Its exclusive interaction with PAP4 has not been reported in other living systems until now and hints at a difference in polyadenylation in plants with respect to mammals and yeast. …

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Role Of P33 In Tombusvirus Replication, Jozsef Stork Jan 2009

Role Of P33 In Tombusvirus Replication, Jozsef Stork

University of Kentucky Doctoral Dissertations

Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In my thesis I describe (i) the effect of phosphorylation of p33, (ii) the RNA chaperone-like activity of p33, and (iii) the role of HSP70s a host proteins in the viral replication. To test the effect of phosphorylation on p33 function, I used in vitro phosphorylated p33. I found that phosphorylation inhibited the ability of p33 to bind to the viral RNA. Phosphorylation-mimicking mutations rendered p33 nonfunctional in plant protoplasts and …

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Evaluating The Effects Of Organic And Conventional Inputs On Soil Chemical And Biological Properties In A Four-Year Vegetable Rotation And The Investigation Of Soil Microbial Properties On Plant Gene Expression, Audrey Law Jan 2009

Evaluating The Effects Of Organic And Conventional Inputs On Soil Chemical And Biological Properties In A Four-Year Vegetable Rotation And The Investigation Of Soil Microbial Properties On Plant Gene Expression, Audrey Law

University of Kentucky Doctoral Dissertations

The objective of this research was to determine the effects of conventional inputs on soil chemical and biological properties compared to organic systems in a four year vegetable rotation. Tillage and cover crops were the same in all treatments to avoid confounding factors often present in similar research. Additional experiments investigated plant gene expression in organic and conventional management systems and in soils with decreased microbial diversity. Experimental plots were prepared in the spring of 2004; four replications of three management treatments, organic, low-input and conventional, were arranged in a randomized complete block design. The rotation consisted of edamame soybean, …

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Identification And Characterization Of Host Factors Involved In Tombusvirus Replication, Yi Jiang Jan 2009

Identification And Characterization Of Host Factors Involved In Tombusvirus Replication, Yi Jiang

University of Kentucky Doctoral Dissertations

Positive strand RNA viruses are intracellular parasites, and their genome replication and infection involves complex virus-host interactions. Therefore, identification of host factors and dissection of their functions during virus replication could facilitate our understanding of the mechanism of virus infection. Those host factors may also provide new targets for viral disease control. Tomato bushy stunt virus (TBSV) has recently become one of the model viruses to study positive strand RNA virus replication and hostvirus interactions. To identify host factors involved in TBSV replication we used yeast as a model host. Co-expression of the replication proteins and a replicon RNA (DI …

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Comparison Of Rope-Wick And Broadcast Treatments For Control Of Canada Thistle And Tall Ironweed, Daisy M. Fryman Jan 2009

Comparison Of Rope-Wick And Broadcast Treatments For Control Of Canada Thistle And Tall Ironweed, Daisy M. Fryman

University of Kentucky Master's Theses

Tall ironweed (Vernonia altissima) and Canada thistle (Cirsium arvense) control in cool season grass pastures was evaluated in 2007 and 2008. Tall ironweed was evaluated in Fayette and Boone Counties, KY and Canada thistle was evaluated at Spindletop Research Farm. Herbicides applied selectively with a rope-wick were compared to a broadcast foliar spray. Treatments were a broadcast treatment, of aminopyralid + 2, 4-D and six rope-wick treatments: aminopyralid at three concentrations, glyphosate, triclopyr and clopyralid at one concentration each. The Boone County location had five broadcast foliar treatments: aminopyralid at three rates, triclopyr + fluroxpyr, and …

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Characterizing The Variability Of Physical And Chemical Properties Across The Soil Individuals Mapped As Amy Silt Loam Soils In Southeastern Arkansas, B. Bhandari, Robert L. Ficklin Jan 2009

Characterizing The Variability Of Physical And Chemical Properties Across The Soil Individuals Mapped As Amy Silt Loam Soils In Southeastern Arkansas, B. Bhandari, Robert L. Ficklin

Journal of the Arkansas Academy of Science

Knowledge of physical and chemical properties of soil is relevant for landowners, researchers, and foresters, so that appropriate crop species and management practices to maximize site productivity can be selected. In addition to issues of plant productivity, the need for assessing soil properties has been expanded due to public interest in determining the consequences of management practices on soil quality relative to sustainability of crop ecosystem functions. The USDA-Natural Resources Conservation Service (NRCS) delineated soil mapping units to provide information about physical and chemical properties of soil in each soil series. However, soil mapping units do not provide details about …

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Sfa Gardens Scrapbook 2005-2009, Sfa Gardens, Stephen F. Austin State University Jan 2009

Sfa Gardens Scrapbook 2005-2009, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Scrapbooks

No abstract provided.

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Accumulation Of A 5′ Proximal Subgenomic Rna Of Citrus Tristeza Virus Is Correlated With Encapsidation By The Minor Coat Protein, Siddarame Gowda, Satyanarayana Tatineni, Svetlana Y. Folimonova, Mark E. Hilf, William O. Dawson Jan 2009

Accumulation Of A 5′ Proximal Subgenomic Rna Of Citrus Tristeza Virus Is Correlated With Encapsidation By The Minor Coat Protein, Siddarame Gowda, Satyanarayana Tatineni, Svetlana Y. Folimonova, Mark E. Hilf, William O. Dawson

Department of Plant Pathology: Faculty Publications

During replication, Citrus tristeza virus (CTV) produces large amounts of two unusual subgenomic (sg) RNAs that are positive-stranded and 5′ coterminal. Although these RNAs are produced in similar amounts and are similar in size, with LMT1 (~750 nt) only slightly larger than LMT2 (~650), we found that the similar sgRNAs are produced differently. We previously showed that the LMT1 RNA is produced by premature termination during genomic RNA synthesis. However, LMT2 production was found to correlate with virion assembly instead of RNA replication. The time course of accumulation of the LMT2 RNA occurred late, coinciding with virion accumulation. The long …

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Vegetable Growing : A Guide For Home Gardeners In Western Australia, Department Of Agriculture, Western Australia Jan 2009

Vegetable Growing : A Guide For Home Gardeners In Western Australia, Department Of Agriculture, Western Australia

Bulletins 4000 -

DAFWAs popular publication about vegetable growing for home gardeners.

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Demographics And Habitat Selection For The Western Meadowlark (Sturnella Neglecta) In The Nebraska Sandhills, Matthew D. Giovanni Jan 2009

Demographics And Habitat Selection For The Western Meadowlark (Sturnella Neglecta) In The Nebraska Sandhills, Matthew D. Giovanni

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The prairie ecosystems of the Great Plains region in North America have largely been replaced and fragmented with industrial agriculture and invasive herbaceous and woody plant species. The concurrent and large-scale suppression of wildfire and elimination of grazing by native ungulates may have further decreased the availability and quality of habitat for wildlife. Indeed, 2004 estimates indicate only 30% of historic grasslands in the Great Plains still exist, while the trends of decreased land area enrolled in the Conservation Reserve Program and increased land area in commercial agriculture indicate continued loss of habitat. This decrease in habitat availability continues to …

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Identification Of Various Testicular Cell Populations In Pubertal And Adult Cockerels, J. Mucksová, J. P. Brillard, J. Hejnar, M. Poplštein, J. Kalina, M. Bakst, H. Yan, P. Trefil Jan 2009

Identification Of Various Testicular Cell Populations In Pubertal And Adult Cockerels, J. Mucksová, J. P. Brillard, J. Hejnar, M. Poplštein, J. Kalina, M. Bakst, H. Yan, P. Trefil

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Precise identification of the male germinal stem cell population is important for their practical use in programs dedicated to the integration of exogenous genetic material in testicular tissues. In the present study, our aim was to identify germinal cell populations in the testes of pubertal and adult cockerels based on the detection of the nuclear DNA content by fluorescence-activated cell sorting (FACS) and on the expression of the Dazl and Stra8 genes in singlecell suspensions of testicular tissues. Cells with a tetraploid DNA content (4c) represent a small and equal fraction of the total germinal cell population in both pubertal …

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Microsatellites Reveal Genetic Diversity In Rotylenchulus Reniformis Populations, R. S. Arias, Salliana R. Stetina, Jennifer L. Tonos, Jodi A. Scheffler, Brian E. Scheffler Jan 2009

Microsatellites Reveal Genetic Diversity In Rotylenchulus Reniformis Populations, R. S. Arias, Salliana R. Stetina, Jennifer L. Tonos, Jodi A. Scheffler, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Rotylenchulus reniformis is the predominant parasitic nematode of cotton in the Mid South area of the United States. Although variable levels of infection and morphological differences have been reported for this nematode, genetic variability has been more elusive. We developed microsatellite-enriched libraries for R. reniformis, produced 1152 clones, assembled 694 corstigs, detected 783 simple sequence repeats (SSR) and designed 192 SSR-markers. The markers were tested on six R. reniformis cultures from four states, Texas, Louisiana, Mississippi and Georgia, in the USA. Based oil performance we selected 156 SSR markers for R. reniformis from which 88 were polymorphic across the …

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Wus And Stm-Based Reporter Genes For Studying Meristem Development In Poplar, Y. Bao, P. Dharmawardhana, R. S. Arias, M. B. Allen, C. Ma, Steven H. Strauss Jan 2009

Wus And Stm-Based Reporter Genes For Studying Meristem Development In Poplar, Y. Bao, P. Dharmawardhana, R. S. Arias, M. B. Allen, C. Ma, Steven H. Strauss

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We describe the development of a reporter system for monitoring meristem initiation in poplar using promoters of poplar homologs to the meristem-active regulatory genes WUSCHEL (WUS) and SHOOTMERISTEMLESS (STM). When ~3 kb of the 5' flanking regions of close homologs were used to drive expression of the GUSPlus gene, 50–60% of the transgenic events showed expression in apical and axillary meristems. However, expression was also common in other organs, including in leaf veins (40 and 46% of WUS and STM transgenic events, respectively) and hydathodes (56% of WUS transgenic events). Histochemical GUS staining of explants during callogenesis and shoot …

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Particle Size Effects On The Quality Of Flour Tortillas Enriched With Whole Grain Waxy Barley, Naruemon Prasopsunwattana, Marfa Botero Omary, Elizabeth A. Arndt, Peter H. Cooke, Rolando A. Flores, Wallace Yokoyama, Ayako Toma, Sutida Chongcham, Sylvia P. Lee Jan 2009

Particle Size Effects On The Quality Of Flour Tortillas Enriched With Whole Grain Waxy Barley, Naruemon Prasopsunwattana, Marfa Botero Omary, Elizabeth A. Arndt, Peter H. Cooke, Rolando A. Flores, Wallace Yokoyama, Ayako Toma, Sutida Chongcham, Sylvia P. Lee

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Wheat tortillas were enriched with whole barley flour (WBF) of different particle sizes including 237 µm (regular [RD. 131 µm (intermediate [IM]). and 68 µm (microground [MG], Topographical and fluorescent microstructure images of flours, doughs, and tortillas were examined, Flours and tortillas were analyzed for color, protein, ash, starch, moisture. and β-glucan content. Farinograph testing was conducted on the flour blends, Water activity and texture analyses of tortillas were conducted, A 9-point hedonic scale was used by 95 untrained panelists to evaluate tortilla appearance, color, flavor, texture, and overall acceptability, Two commercial products (CP) were included in …

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Effects Of Spontaneous Heating On Fiber Composition, Fiber Digestibility, And In Situ Disappearance Kinetics Of Neutral Detergent Fiber For Alfalfa-Orchardgrass Hays, W. K. Coblentz, P. C. Hoffman Jan 2009

Effects Of Spontaneous Heating On Fiber Composition, Fiber Digestibility, And In Situ Disappearance Kinetics Of Neutral Detergent Fiber For Alfalfa-Orchardgrass Hays, W. K. Coblentz, P. C. Hoffman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

During 2006 and 2007, forages from 3 individual hay harvests were utilized to assess the effects of spontaneous heating on concentrations of fiber components, 48-h neutral detergent fiber (NDF) digestibility (NDFD), and in situ disappearance kinetics of NDF for large-round bales of mixed alfalfa (Medicago sativa L.) and orchardgrass (Dactylis glomerata L.). Over the 3 harvests, 96 large-round bales were made at preset bale diameters of 0.9, 1.2, or 1.5 m, and at moisture concentrations ranging from 9.3 to 46.6%. Internal bale temperatures were monitored daily during an outdoor storage period, reaching maxima (MAX) of 77.2°C and 1,997 heating degree …

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A Whole-Genome Assembly Of The Domestic Cow, Bos Taurus, Aleksey V. Zimin, Arthur L. Delcher, Liliana Florea, David R. Kelley, Michael C. Schatz, Daniela Puiu, Finnian Hanrahan, Geo Pertea, Curtis P. Van Tassell, Tad S. Sonstegard, Guillaume Marçais, Michael Roberts, Poorani Subramanian, James A. Yorke, Steven. L Salzberg Jan 2009

A Whole-Genome Assembly Of The Domestic Cow, Bos Taurus, Aleksey V. Zimin, Arthur L. Delcher, Liliana Florea, David R. Kelley, Michael C. Schatz, Daniela Puiu, Finnian Hanrahan, Geo Pertea, Curtis P. Van Tassell, Tad S. Sonstegard, Guillaume Marçais, Michael Roberts, Poorani Subramanian, James A. Yorke, Steven. L Salzberg

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: The genome of the domestic cow, Bos Taurus, was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods.

Results: We have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques, creating an assembly of 2.86 billion base pairs that has multiple improvements over previous assemblies: it is more complete, covering more of the genome; thousands of gaps have been closed; many erroneous inversions, deletions, and translocations have been corrected; and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more accurate …

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Expression And Functional Characterization Of The Plant Antimicrobial Snakin-1 And Defensin Recombinant Proteins, Natalia Kovalskaya, Rosemarie W. Hammond Jan 2009

Expression And Functional Characterization Of The Plant Antimicrobial Snakin-1 And Defensin Recombinant Proteins, Natalia Kovalskaya, Rosemarie W. Hammond

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study, for the first time, functionally active, recombinant, cysteine-rich plant proteins snakin-1 (SN1) and defensin (PTH1) were expressed and purified using a prokaryotic expression system. The overall level of antimicrobial activities of SN1 and PTH1 produced in Escherichia coli was commensurate with that of the same proteins previously obtained from plant tissues. Both proteins exhibited strong antibacterial activity against the phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (50% inhibitory concentration (IC50) 1.5–8 lM) and antifungal activity against the phytopathogenic fungi Colletotrichum coccoides and Botrytis cinerea (IC50 5–14 lM). Significantly weaker activity was observed against Pseudomonas syringae …

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Open Field Host Selection And Behavior By Tamarisk Beetles (Diorhabda Spp.) (Coleoptera: Chrysomelidae) In Biological Control Of Exotic Saltcedars (Tamarix Spp.) And Risks To Non-Target Athel (T. Aphylla) And Native Frankenia Spp., Patrick J. Moran, C. Jack Deloach, Tom L. Dudley, Joaquin Sanabria Jan 2009

Open Field Host Selection And Behavior By Tamarisk Beetles (Diorhabda Spp.) (Coleoptera: Chrysomelidae) In Biological Control Of Exotic Saltcedars (Tamarix Spp.) And Risks To Non-Target Athel (T. Aphylla) And Native Frankenia Spp., Patrick J. Moran, C. Jack Deloach, Tom L. Dudley, Joaquin Sanabria

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Biological control of invasive saltcedars (Tamarix spp.) in the western U.S. by exotic tamarisk leaf beetles, Diorhabda spp., first released in 2001 after 15 years of development, has been successful. In Texas, beetles from Crete, Greece were first released in 2004 and are providing control. However, adults alight, feed and oviposit on athel (Tamarix aphylla), an evergreen tree used for shade and as a windbreak in the southwestern U.S. and México, and occasionally feed on native Frankenia spp. plants. The ability of tamarisk beetles to establish on these potential field hosts was investigated in the field. In …

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