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Chemical Constituents Of Aglaia Lanuginose. King, Fadzly Adzhar Kamarulzaman Jan 2010

Chemical Constituents Of Aglaia Lanuginose. King, Fadzly Adzhar Kamarulzaman

Student Works (2010-2019)

Aglaia is a small genus of trees that belong to the family Meliaceae. They are widely distributed in India, Malay Peninsula and throughout Indomalesia. The chemistry of Aglaia has been extensively investigated and most of the researches done on these plants focused on their effect as insecticides, antifeedant, growth inhibitors, cytotoxicity and others. The dichloromethane extract from the bark of Aglaia lanuginose were studied. The chromatographic separation on both of the extracts gave ten compounds. The compounds isolated were identified as eichlerialactone 102, methyl eichlerianate 103, cabraleone 104, ocotillone 105, 4-hydroxycinnamyl acetate 106, cabralealactone 107, eichlerianic acid 108, shoreic acid …

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Computational Identification Of Micro Rna In Musa Sp Est Sequences., Tan Yew Seong Jan 2010

Computational Identification Of Micro Rna In Musa Sp Est Sequences., Tan Yew Seong

Student Works (2010-2019)

MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search based approach has been used for identifying the novel miRNA genes of Musa sp. using the BLAST program with a total of 42,978 Musa sp. expressed sequence tags (EST) were compared to a total of 959 previously known plant miRNA sequences. Forty-seven candidate miRNA with homology to previously known miRNAs were identified. By using the mFold3.1 program, …

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Bioactivity And Chemical Investigations Of Pereskia Bleo And Pereskia Grandifollia., Sim Kae Shin Jan 2010

Bioactivity And Chemical Investigations Of Pereskia Bleo And Pereskia Grandifollia., Sim Kae Shin

Student Works (2010-2019)

Ethnopharmacological data has been one of the common useful criteria in drug discovery. Pereskia bleo and Pereskia grandifolia (Cactaceae), commonly known as ‘Jarum Tujuh Bilah’ in Malay and ‘Cak Sing Cam’ in Chinese have long been used as natural remedies in Malaysia. The experimental approach in the present study was based on bioassay-guided fractionation. The crude methanol and fractionated extracts of both Pereskia spp. were initially investigated for their biological activities such as antioxidant, antimicrobial and cytotoxic effect against five human cancer cell lines, namely nasopharyngeal epidermoid carcinoma cell line (KB), cervical carcinoma cell line (CasKi), colon carcinoma cell line …

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Chemical Constituents Of Cryptocarya Densiflora., Nurul Nazneem Wan Othman Jan 2010

Chemical Constituents Of Cryptocarya Densiflora., Nurul Nazneem Wan Othman

Student Works (2010-2019)

The extraction of alkaloids from the Malaysian Cryptocarya densiflora has been carried out in this study. The alkaloids were extracted from the bark and leaves of this species using acid base extraction and the crude alkaloids obtained were subjected to extensive chromatographic techniques such as thin layer and column chromatography. The structural elucidation of the purified alkaloids were performed with the aid of spectroscopic methods i.e. 1H NMR, 13C NMR, 2D NMR, IR, UV and LCMS. Investigation of the alkaloidal content from the bark of Cryptocarya densiflora afforded six alkaloids and their structures were elucidated as laurotetanine 63, isocaryachine 64, …

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Agrobacterium-Mediated Transformation Of Cryptocoryne Willisii With Gus And Mgfp 5 Reporter Genes / Wong Sher Ming, Wong Sher Ming Jan 2010

Agrobacterium-Mediated Transformation Of Cryptocoryne Willisii With Gus And Mgfp 5 Reporter Genes / Wong Sher Ming, Wong Sher Ming

Student Works (2010-2019)

In this study, a simple method using Murashige and Skoog basal medium (1962) supplemented with 6-BA alone was developed for direct multiple shoots induction from shoot base culture of C. willisii. Shoots proliferation and elongation were achieved on a single medium without the need of subculturing. The optimum concentration of 6-BA for direct shoots regeneration of C. willisii was investigated. It was found to be 6 mg/ L. Optimum media was then used for regenerate and propagate of transformed C. willisii. On the other hand, ransformation of C. willisii with Agrobacterium tumefaciens strain LBA4404 harbouring pCAMBIA1304 was performed. Effects on …

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In Vitro Cultures For Production Of Zerumbone And Mass Propagation In Zingiber Zerumbet Smith., Farah Diana Idris Jan 2010

In Vitro Cultures For Production Of Zerumbone And Mass Propagation In Zingiber Zerumbet Smith., Farah Diana Idris

Student Works (2010-2019)

Lempoyang (Zingiber zerumbet Smith.) is an important plant which has been propagated because of its medicinal values. Lempoyang is widely used as a folk medicine in Asian countries such as Malaysia, Indonesia, India and Taiwan. Zerumbone, a cyclic sesqueterpene found to be the major and main bioactive compound that gives great potential in medicinal purposes especially in the treatment of chronic diseases based on recent reports. Recently, it was found that zerumbone inhibited the growth of human leukemia cell line, HL-60 cell. Even though there are so many great values contain in the lempoyang but it is hard to obtain …

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Effects Of Moist Chilling, Gibberellic Acid, And Scarification On Seed Dormancy In The Rare Endemic Pedicularis Olympica (Scrophulariaceae), Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan, Fatma Selcen Sakar Jan 2010

Effects Of Moist Chilling, Gibberellic Acid, And Scarification On Seed Dormancy In The Rare Endemic Pedicularis Olympica (Scrophulariaceae), Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan, Fatma Selcen Sakar

Turkish Journal of Botany

We investigated the germination requirements of a rare endemic plant species, Pedicularis olympica Boiss. (Scrophulariaceae), which grows in wet habitats on Uludağ Mountain, Turkey. We studied the effects of scarification, moist chilling (+4 °C) for 15 days, different doses of gibberellic acid (GA_{3}; 100, 150, and 250 ppm) and combined hormone and moist chilling treatments under dark (20 °C) and photoperiod (20/10 °C; 12/12 h, respectively) conditions. GA_{3} was able to break dormancy. The highest germination rate was found in the seeds treated with 250 ppm GA_{3}; 64% of these seeds germinated when treated with moist chilling and incubated in …

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Seed Morphology Of Ebenus L. Species Endemic To Turkey, Fatma Bayrakdar, Zeki̇ Aytaç, Zeki̇ye Suludere, Selami̇ Candan Jan 2010

Seed Morphology Of Ebenus L. Species Endemic To Turkey, Fatma Bayrakdar, Zeki̇ Aytaç, Zeki̇ye Suludere, Selami̇ Candan

Turkish Journal of Botany

In the present study, seed surface morphology of species of the genus Ebenus L. in Turkey, all of which are endemic, was studied. E. plumosa var. speciosa has the biggest and E. laguroides var. laguroides has the smallest seeds. The seed shapes are spheroid in E. reesei, orbiculate in E. pisidica, and oblong-spheroid in others. Ornamentation is striate in E. macrophylla, rugose in E. haussknechtii, and reticulate or multi-reticulate in others. Microphylle is subterminal in all Turkish Ebenus species.

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Pollen Morphology Of The Genus Fritillaria L. (Liliaceae) In Turkey, Mehtap Tekşen, Zeki̇ Aytaç, Nur Münevver Pinar Jan 2010

Pollen Morphology Of The Genus Fritillaria L. (Liliaceae) In Turkey, Mehtap Tekşen, Zeki̇ Aytaç, Nur Münevver Pinar

Turkish Journal of Botany

Pollen grains of 39 taxa of the genus Fritillaria L. in Turkey were examined by light and scanning electron microscopy. Detailed pollen morphological characteristics are given for these taxa. Our investigation shows that the sculpturing of the exine provides valuable characters for separating species. In particular, sculpturing of the exine at the proximal face turned out to be taxonomically important. Six types of ornamentation were determined: reticulate, reticulate-perforate, suprareticulate, rugulate-reticulate, psilate-perforate, and perforate. On the basis of exine sculpturing, the sulcus membrane, and the apex sulcus, 5 main pollen types are recognised.

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Classification Of Some Plant Species According To Grime’S Strategies In A Quercus Cerris L. Var. Cerris Woodland In Samsun, Northern Turkey, Mahmut Kilinç, Nesli̇han Karavi̇n, Hamdi̇ Güray Kutbay Jan 2010

Classification Of Some Plant Species According To Grime’S Strategies In A Quercus Cerris L. Var. Cerris Woodland In Samsun, Northern Turkey, Mahmut Kilinç, Nesli̇han Karavi̇n, Hamdi̇ Güray Kutbay

Turkish Journal of Botany

This study was carried out on 2 permanent plots of Quercus cerris L. var. cerris woodland in the Kurupelit region of Samsun, northern Turkey. Grime's CSR strategies were determined for 85 plant species from the 2 study plots (61 and 50 species from plot 1 and plot 2, respectively) according to the method that was applied. Most of the plant species in both plots had secondary and tertiary strategies, and this suggests that plants were exposed to more than one pressure factor. In both plots, CR (competitor-ruderal) was the most common strategy.

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The Diatoms Of Asartepe Dam Lake (Ankara), With Environmental And Some Physicochemical Properties, Tahi̇r Atici, Emi̇ne Olcay Obali Jan 2010

The Diatoms Of Asartepe Dam Lake (Ankara), With Environmental And Some Physicochemical Properties, Tahi̇r Atici, Emi̇ne Olcay Obali

Turkish Journal of Botany

The shoreline benthic diatoms of Asartepe Dam Lake were identified from different habitats (epipelic, epiphytic, and epilithic) at 7 sampling stations between April 2003 and June 2004. In the investigation, a total of 93 taxa were identified. Of those, Cymbella affinis Kütz., Navicula radiosa Kütz., Ulnaria ulna (Nitzsch) Compère, Pinnularia borealis Ehrenb., Amphora ovalis (Kütz.) Kütz., Cyclotella ocellata Pant. and Nitzschia dissipata (Kütz.) Grunow were observed and abundant at all stations. Some physical and chemical water quality parameters were measured and correlated with biological parameters.

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Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson Jan 2010

Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in …

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Drivers Impacting The Adoption Of Sustainable Agricultural Management Practices And Production Systems Of The Northeast And Southeast United States, G. F. Sassenrath, J. M. Halloran, D. Archer, R. L. Raper, J. Hendrickson, P. Vadas, J. Hanson Jan 2010

Drivers Impacting The Adoption Of Sustainable Agricultural Management Practices And Production Systems Of The Northeast And Southeast United States, G. F. Sassenrath, J. M. Halloran, D. Archer, R. L. Raper, J. Hendrickson, P. Vadas, J. Hanson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Agricultural production responds to social, political, economic, environmental, and technological drivers that influence producers’ decisions and shape the individual systems through modification of management practices, crop and livestock mix, and marketing strategy. We use an interview and discussion approach with producer panels to examine production systems in the eastern United States and explore key drivers impacting their unique characteristics and development. The internal social driver that values the farming lifestyle is a principle factor that leads people to choose farming. Irrespective of location, farming is first and foremost a lifestyle choice. The choice of type of production system is partly …

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Cloning, Expression, And Immunogenicity Of Flavobacterium Columnare Heat Shock Protein Dnaj, Oscar Olivares-Fuster, Jeffery S. Terhune, Craig A. Shoemaker, Covadonga R. Arias Jan 2010

Cloning, Expression, And Immunogenicity Of Flavobacterium Columnare Heat Shock Protein Dnaj, Oscar Olivares-Fuster, Jeffery S. Terhune, Craig A. Shoemaker, Covadonga R. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Flavobacterium columnare heat shock protein (HSP) gene dnaJ* was isolated, cloned, expressed, and used as an antigen in a recombinant vaccine strategy for channel catfish Ictalurus punctatus. The F. columnare dnaJ* sequence was obtained from genomovars I and II and showed intraspecies variability. Recombinant protein was expressed and purified from Escherichia coli cultures and injected intraperitoneally (12 lg of purified DnaJ/fish) into fingerling channel catfish. In addition, induced (expressing the recombinant DnaJ) and uninduced (no recombinant protein being produced) E. coli cultures were also used to immunize fish. At 28 d postimmunization, antibody response was evaluated and the …

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Production Characteristics And Body Composition Of Juvenile Cobia Fed Three Different Commercial Diets In Recirculating Aquaculture Systems, Charles R. Weirich, Paul S. Wills, Richard M. Baptiste, Marty A. Riche Jan 2010

Production Characteristics And Body Composition Of Juvenile Cobia Fed Three Different Commercial Diets In Recirculating Aquaculture Systems, Charles R. Weirich, Paul S. Wills, Richard M. Baptiste, Marty A. Riche

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effect of feeding three commercial diets on production characteristics and body composition of juvenile cobia Rachycentron canadum reared using recirculating aquaculture systems (RASs) was assessed in a 56-d growth trial. Juvenile cobia (mean weight ± SE, 29.2 ± 0.7 g) were stocked into three 8-m3 tanks in each of four RASs at an initial density of 1.2 kg/m3. After stocking, fish were fed one of three commercial diets: Hybrid Striped Bass diet (HSB; 44% protein, 12% lipid), Finfish Gold diet (GOLD; 42% protein, 16% lipid), or Marine Grower diet (MG; 50% protein, 15% lipid), all at …

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Comparisons Of Sperm Storage Tubule Distribution And Number In 4 Strains Of Mature Broiler Breeders And In Turkey Hens Before And After The Onset Of Photostimulation, M. R. Bakst, A. M. Donoghue, D. E. Yoho, J. R. Moyle, S. M. Whipple, M. J. Camp, G. Q. Liu, R. K. Bramwell Jan 2010

Comparisons Of Sperm Storage Tubule Distribution And Number In 4 Strains Of Mature Broiler Breeders And In Turkey Hens Before And After The Onset Of Photostimulation, M. R. Bakst, A. M. Donoghue, D. E. Yoho, J. R. Moyle, S. M. Whipple, M. J. Camp, G. Q. Liu, R. K. Bramwell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The biological basis of sustained fertility in broiler and turkey hens is their capacity to store sperm in the oviductal sperm storage tubules (SST) located in the uterovaginal junction. The objectives of this study were to determine if the numbers of SST varied between 4 strains of broiler breeders and determine the number of SST in the turkey before (less than 9 d of photostimulation) and after (up to 22 d of photostimulation and laying) photostimulation. No statistical differences were observed in SST numbers in the 4 strains of broilers examined or in turkey hens before and after the onset …

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A Comparison Of Cumulative-Germination Response Of Cheatgrass (Bromus Tectorum L.) And Five Perennial Bunchgrass Species To Simulated Field-Temperature Regimes, Stuart P. Hardegree, Corey A. Moffet, Bruce A. Roundy, Thomas A. Jones, Stephen J. Novak, Patrick E. Clark, Frederick B. Pierson, Gerald N. Flerchinger Jan 2010

A Comparison Of Cumulative-Germination Response Of Cheatgrass (Bromus Tectorum L.) And Five Perennial Bunchgrass Species To Simulated Field-Temperature Regimes, Stuart P. Hardegree, Corey A. Moffet, Bruce A. Roundy, Thomas A. Jones, Stephen J. Novak, Patrick E. Clark, Frederick B. Pierson, Gerald N. Flerchinger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cheatgrass (Bromus tectorum L.) has come to dominate millions of hectares of rangeland in the Intermountain western United States. Previous studies have hypothesized that one mechanism conferring a competitive advantage to this species is the ability to germinate rapidly at low temperatures in the fall, winter and spring and, therefore, initiate growth and establishment more rapidly than more desirable perennial bunchgrass species. In this experiment, we developed thermal-germination-response models for multiple seedlots of cheatgrass and five perennial grass species. We conducted sensitivity analysis on potential-cumulative-germination response to a 38-year simulation of field-variable conditions of seedbed temperature and moisture. Cheatgrass …

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Groundwater Nutrient Concentrations During Prairie Reconstruction On An Iowa Landscape, M.D. Tomer, K.E. Schilling, C.A. Cambardella, P. Jacobson, P. Drobney Jan 2010

Groundwater Nutrient Concentrations During Prairie Reconstruction On An Iowa Landscape, M.D. Tomer, K.E. Schilling, C.A. Cambardella, P. Jacobson, P. Drobney

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

One anticipated benefit of ecosystem restoration is water quality improvement. This study evaluated NO3-N and phosphorus in subsurface waters during prairie establishment following decades of row-crop agriculture. A prairie seeding in late 2003 became established in 2006. Wells and suction cup samplers were monitored for NO3-N and phosphorus. Nitrate-N varied with time and landscape position. Nondetectable NO3-N concentrations became modal along ephemeral drainageways in 2006, when average concentrations in uplands first became <10mg NO3-N L−1. This decline continued and upland groundwater averaged near 2mg NO3-N L−1 after 2007. The …

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Optimized Construction Of Microsatellite-Enriched Libraries, Natascha Techen, R. S. Arias, Neil C. Glynn, Zhiqiang Pan, Ikhlas A. Khan, Brian E. Scheffler Jan 2010

Optimized Construction Of Microsatellite-Enriched Libraries, Natascha Techen, R. S. Arias, Neil C. Glynn, Zhiqiang Pan, Ikhlas A. Khan, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The construction of microsatellite-enriched libraries is an indispensable tool to search for molecular markers as complete genome sequences are still not available for the majority of species of interest. Numerous protocols are available in the literature for the construction of these libraries; however, sometimes their low efficiency or lack of optimization in the protocols can restrict their efficacy. We have designed and tested various adapters and ligation methods; we also tested oligo-repeat combinations and hybridization temperatures, and created libraries with this new protocol for four organisms: Ipomoea batatas (L.) Lam, Chionanthus retusus Lindley & Paxton, Rotylenchulus reniformis Linford & Olivera …

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Genetic Improvement In Winter Wheat Yields In The Great Plains Of North America, 1959–2008, Robert A. Graybosch, C. James Peterson Jan 2010

Genetic Improvement In Winter Wheat Yields In The Great Plains Of North America, 1959–2008, Robert A. Graybosch, C. James Peterson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Data from USDA-coordinated winter wheat (Triticum aestivum L.) regional performance nurseries collected over the time period 1959 to 2008 were used to estimate genetic gain (loss) in grain yield, grain volume weight, days to heading, and plant height in winter wheats adapted to the Great Plains of North America. In both the Southern Regional (SRPN) and Northern Regional Performance Nurseries (NRPN), linear regression revealed significant positive relationships between relative grain yields of advanced breeding lines and calendar year of the nursery trial. The estimated genetic gain in grain yield potential since 1959 was approximately 1.1% (of the control cultivar …

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Novel Carboxamides As Potential Mosquito Repellents, Alan R. Katritzky, Zuoquan Wang, Svetoslav Slavov, Dimitar A. Dobchev, C. Dennis Hall, Maia Tsikolia, Ulrich R. Bernier, Natasha M. Elejalde, Gary G. Clark, Kenneth J. Linthicum Jan 2010

Novel Carboxamides As Potential Mosquito Repellents, Alan R. Katritzky, Zuoquan Wang, Svetoslav Slavov, Dimitar A. Dobchev, C. Dennis Hall, Maia Tsikolia, Ulrich R. Bernier, Natasha M. Elejalde, Gary G. Clark, Kenneth J. Linthicum

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A model was developed using 167 carboxamide derivatives, from the United States Department of Agriculture archival database, that were tested as arthropod repellents over the past 60 yr. An artificial neural network employing CODESSA PRO descriptors was used to construct a quantitative structure-activity relationship model for prediction of novel mosquito repellents. By correlating the structure of these carboxamides with complete protection time, a measure of repellency based on duration, 34 carboxamides were predicted as candidate mosquito repellents. There were four additional compounds selected on the basis of their structural similarity to those predicted. The compounds were synthesized either by reaction …

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Potential For Stable Flies And House Flies (Diptera: Muscidae) To Transmit Rift Valley Fever Virus1, Michael J. Turell, David J. Dohm, Christopher J. Geden, Jerome A. Hogsette, Kenneth Linthicum Jan 2010

Potential For Stable Flies And House Flies (Diptera: Muscidae) To Transmit Rift Valley Fever Virus1, Michael J. Turell, David J. Dohm, Christopher J. Geden, Jerome A. Hogsette, Kenneth Linthicum

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Rift Valley fever (RVF), a disease of ruminants and humans, has been responsible for large outbreaks in Africa that have resulted in hundreds of thousands of human infections and major economic disruption due to loss of livestock and to trade restrictions. As indicated by the rapid spread of West Nile viral activity across North America since its discovery in 1999 and the rapid and widespread movement of chikungunya virus from Africa throughout the Indian Ocean Islands to Asia and Europe, an introduced exotic arbovirus can be rapidly and widely established across wide geographical regions. Although RVF virus (RVFV) is normally …

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Efficacy Of Commercial Mosquito Traps In Capturing Phlebotomine Sand Flies (Diptera: Psychodidae) In Egypt, D. F. Hoel, D. L. Kline, Jerome Hogsette, Ulrich R. Bernier, S. S. El-Hossary, H. A. Hanafi, N. Watany, E. Y. Fawaz, B. D. Furman, P. J. Obenauer, D. E. Szumlas Jan 2010

Efficacy Of Commercial Mosquito Traps In Capturing Phlebotomine Sand Flies (Diptera: Psychodidae) In Egypt, D. F. Hoel, D. L. Kline, Jerome Hogsette, Ulrich R. Bernier, S. S. El-Hossary, H. A. Hanafi, N. Watany, E. Y. Fawaz, B. D. Furman, P. J. Obenauer, D. E. Szumlas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Four types of commercial mosquito control traps, the Mosquito Magnet Pro (MMP), the Sentinel 360 (S360), the BG-Sentinel (BGS), and the Mega-Catch Ultra (MCU), were compared with a standard Centers for Disease Control and Prevention (CDC) light trap for efficacy in collecting phlebotomine sand flies (Diptera: Psychodidae) in a small farming village in the Nile River Valley 10 km north of Aswan, Egypt. Each trap was baited with either carbon dioxide (CO2) from combustion of butane gas (MMP), dry ice (CDC and BGS traps), light (MCU and S360), or dry ice and light (CDC). Traps were rotated through …

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Monitoring And Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment Of Switchgrass (Panicum Virgatum L.), Rohit Arora, Chithra Manisseri, Chenlin Li, Markus D. Ong, Henrik Vibe Scheller, Kenneth P. Vogel, Blake A. Simmons, Seema Singh Jan 2010

Monitoring And Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment Of Switchgrass (Panicum Virgatum L.), Rohit Arora, Chithra Manisseri, Chenlin Li, Markus D. Ong, Henrik Vibe Scheller, Kenneth P. Vogel, Blake A. Simmons, Seema Singh

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fundamental understanding of biomass pretreatment and its influence on saccharification kinetics, total sugar yield, and inhibitor formation is essential to develop efficient next-generation biofuel strategies, capable of displacing fossil fuels at a commercial level. In this study, we investigated the effect of residence time and temperature during ionic liquid (IL) pretreatment of switchgrass using 1-ethyl-3-methyl imidazolium acetate. The primary metrics of pretreatment performance are biomass delignification, xylan and glucan depolymerization, porosity, surface area, cellulase kinetics, and sugar yields. Compositional analysis and quantification of process streams of saccharides and lignin demonstrate that delignification increases as a function of pretreatment temperature and …

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Comparison Of Broiler Litter And Commercial Fertilizer At Equivalent N Rates On Soil Properties, Ardeshir Adeli, Haile Tewolde, Karamat Sistani, Dennis Rowe Jan 2010

Comparison Of Broiler Litter And Commercial Fertilizer At Equivalent N Rates On Soil Properties, Ardeshir Adeli, Haile Tewolde, Karamat Sistani, Dennis Rowe

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A 3-year study was conducted to determine the effects of broiler litter relative to inorganic fertilizer on soil nutrient content and quality in an upland Loring silt loam soil. Treatments included annual broiler litter rates of 0, 2.2, 4.5, 5.6, 6.7, 10.1, and 13.4 Mg ha−1 y−1 and commercial fertilizer rates of 34, 68, 90, 112, 134, and 168 kg nitrogen (N) ha−1 y−1. Broiler litter application linearly increased soil total carbon (C), microbial biomass C, extractable soil phosphorus (P), potassium (K), soil cation exchange capacity (CEC), and the stability of soil aggregate. At the …

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Soil Organic Matter Fractions And Aggregate Distribution In Response To Tall Fescue Stands, I. P. Handayani, Mark S. Coyne, R. S. Tokosh Jan 2010

Soil Organic Matter Fractions And Aggregate Distribution In Response To Tall Fescue Stands, I. P. Handayani, Mark S. Coyne, R. S. Tokosh

Plant and Soil Sciences Faculty Publications

The study was conducted to evaluate the influences of tall fescue management on soil organic matter fractions and macro- and microaggregate distribution. Soil samples were collected from four paired adjacent fields consisting of five years of tall fescue mono and poly stands in Western Kentucky. Soil samples from 0 to 15 cm and 15 to 30 cm soil depths were analyzed for soil organic C and N, particulate organic matter C (POM-C) and N (POM-N), macro- and micro aggregate distribution and C-associated with macro- and micro- aggregates. Significant effects were observed between stands for all the properties, except total C, …

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Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Marce D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried Jan 2010

Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Marce D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried

Department of Entomology: Faculty Publications

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific doublestranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. …

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Isolation Of The Phycodnavirus Pbcv-1 By Biological Laser Printing, Lisa A. Fitzgerald, Peter K. Wu, James Gurnon, Justin C. Biffinger, Bradley R. Ringeisen, James L. Van Etten Jan 2010

Isolation Of The Phycodnavirus Pbcv-1 By Biological Laser Printing, Lisa A. Fitzgerald, Peter K. Wu, James Gurnon, Justin C. Biffinger, Bradley R. Ringeisen, James L. Van Etten

Department of Plant Pathology: Faculty Publications

The Phycodnaviridae family of viruses is diverse genetically but similar morphologically. These viruses infect eukaryotic algal hosts from both fresh and marine waters, and are an important component of aqueous environments. They play important roles in the dynamics of algal blooms, nutrient cycling, algal community structure, and possibly gene transfer between organisms. As such, it is important to identify new viruses within the Phycodnaviridae family. Biological laser printing (BioLP) was used to isolate single virus particles from solution. BioLP prints droplets containing a single virus particle directly onto a host medium, thereby enabling viruses to be isolated from unmodified samples. …

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Microarray Analysis Of Paramecium Bursaria Chlorella Virus 1 Transcription, Giane M. Yanai-Balser, Garry A. Duncan, James D. Eudy, Dong Wang, Xiao Li, Irina V. Agarkova, David Dunigan, James L. Van Etten Jan 2010

Microarray Analysis Of Paramecium Bursaria Chlorella Virus 1 Transcription, Giane M. Yanai-Balser, Garry A. Duncan, James D. Eudy, Dong Wang, Xiao Li, Irina V. Agarkova, David Dunigan, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Paramecium bursaria chlorella virus 1 (PBCV-1), a member of the family Phycodnaviridae, is a large double-stranded DNA, plaque-forming virus that infects the unicellular green alga Chlorella sp. strain NC64A. The 330-kb PBCV-1 genome is predicted to encode 365 proteins and 11 tRNAs. To monitor global transcription during PBCV-1 replication, a microarray containing 50-mer probes to the PBCV-1 365 protein-encoding genes (CDSs) was constructed. Competitive hybridization experiments were conducted by using cDNAs from poly(A)- containing RNAs obtained from cells at seven time points after virus infection. The results led to the following conclusions: (i) the PBCV-1 replication cycle is temporally …

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Morphological And Molecular Characterization Of Discocriconemella Inarata, An Endemic Nematode From North American Native Tallgrass Prairies, Thomas O. Powers, Timothy Harris, Rebecca Higgins, Lisa Sutton, Kirsten S. Powers Jan 2010

Morphological And Molecular Characterization Of Discocriconemella Inarata, An Endemic Nematode From North American Native Tallgrass Prairies, Thomas O. Powers, Timothy Harris, Rebecca Higgins, Lisa Sutton, Kirsten S. Powers

Department of Plant Pathology: Faculty Publications

Discocriconemella inarata, a plant parasitic nematode species originally discovered in a virgin tallgrass prairie in northwest Iowa, was re-examined by molecular and morphological analyses of topotype material. This species has never been recorded in cultivated fields and could potentially serve as an indicator for high quality prairie habitats. DNA sequence from a conserved 3’ portion of the 18S ribosomal gene exhibited an identical match between D. inarata topotype specimens and topotype specimens of Mesocriconema xenoplax from Fresno, California. Higher resolution sequence analyses using the internal transcribed spacer 1 (ITS1) and a portion of the mitochondrial gene cytochrome b (cytb) …

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