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The Effects Of Gibberellic Acid And Kinetin On Overcoming The Effects Of Juglone Stress On Seed Germination And Seedling Growth, İrfan Terzi̇, İsmai̇l Kocaçalişkan Jan 2010

The Effects Of Gibberellic Acid And Kinetin On Overcoming The Effects Of Juglone Stress On Seed Germination And Seedling Growth, İrfan Terzi̇, İsmai̇l Kocaçalişkan

Turkish Journal of Botany

Juglone (5-hydroxy-1,4-naphthoquinone) is an allelochemical responsible for walnut allelopathy. The effects of gibberellic acid (GA_{3}) and kinetin (KIN) on overcoming the effects of juglone stress on seed germination and seedling growth were investigated in barley, wheat, cucumber, alfalfa, and tomato. Seeds pre-treated with plant growth regulators were used to test their effects on the alleviation of juglone stress. It was observed that seed germination in tomato and wheat was inhibited by juglone and that the plant growth regulators alleviated it significantly. Elongation and dry weight of the seedlings of all the species used in the study were reduced significantly by …

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Using Vegetation Units As Salinity Predictors In The Lower Cheliff Algeria, Adda Ababou, Mohammed Chouieb, Mohammed Khader, Khalladi Mederbal, Djamel Saidi Jan 2010

Using Vegetation Units As Salinity Predictors In The Lower Cheliff Algeria, Adda Ababou, Mohammed Chouieb, Mohammed Khader, Khalladi Mederbal, Djamel Saidi

Turkish Journal of Botany

The Lower Cheliff plain is among the largest salted soils in north-western Algeria. In order to establish the relationships between the main soil factors and plant species in this stressed ecosystem, 133 relevés related to soil and vegetation were realized. Soil variables measured included electrical conductivity and calcium carbonate. The 133 relevés were arranged in clusters using k-means classification. The similarity analysis used to examine the variation in vegetation assemblage structure showed significant differences in taxonomical composition among groups of relevés. The phi coefficient of fidelity used then to extract the different vegetation units and to measure species concentration in …

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Morphological And Anatomical Studies Of Annual Taxa Of Sideritis L. (Lamiaceae), With Notes On Chorology In Turkey, Ayşegül Güvenç, Hayri̇ Duman Jan 2010

Morphological And Anatomical Studies Of Annual Taxa Of Sideritis L. (Lamiaceae), With Notes On Chorology In Turkey, Ayşegül Güvenç, Hayri̇ Duman

Turkish Journal of Botany

Sideritis L. (Lamiaceae) includes approximately 150 species of annual and perennial plants distributed mainly in the Mediterranean region. In Turkey, 44 species (55 taxa) are native. Three of these species (5 taxa) are annual and these taxa are included in Burgsdorfia and Hesiodia sections. This study was undertaken to investigate the morphological and anatomical properties of 5 annual taxa of Sideritis. Full descriptions and illustrations of these taxa are given, along with some additional information regarding their ecology and phytogeography. The anatomy of the leaf blade, midrib, and stem of the taxa are described and illustrated.

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Taxonomy Of Campanula Tomentosa Lam. And C. Vardariana Bocquet From Turkey, Emi̇ne Alçitepe, Kemal Yildiz Jan 2010

Taxonomy Of Campanula Tomentosa Lam. And C. Vardariana Bocquet From Turkey, Emi̇ne Alçitepe, Kemal Yildiz

Turkish Journal of Botany

The taxonomical status of Campanula tomentosa Lam. and C. vardariana Bocquet, belonging to the section Quinqueloculares (Boiss.) Phitos, which have important taxonomical problems, was reviewed in this study. Furthermore, morphological, anatomical leaf surface, and palynological features of these 2 species were examined in detail. Habit, shape of leaves, calyx, corolla, stamen, pistil, and seed micromorphological features as morphological characters; and pollen diameter, pore diameter, spinule length, and number, ornamentation, etc. as palynological characters, were examined and discussed. An identification key for the species is presented according to the results obtained. Following this extensive study, the mentioned problems of C. tomentosa …

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Clonal Propagation Of Dorystoechas Hastata Via Axillary Shoot Proliferation, Bengi̇ Erdağ, Yelda Emek, Serap Aydoğan Jan 2010

Clonal Propagation Of Dorystoechas Hastata Via Axillary Shoot Proliferation, Bengi̇ Erdağ, Yelda Emek, Serap Aydoğan

Turkish Journal of Botany

A protocol for the in vitro seed germination, seedling development, and axillary shoot propagation of Dorystoechas hastata Boiss. & Heldr. ex Bentham is described. By employing sterilised seeds that were transferred onto in vitro media, the effects of various in vitro media, photoperiods, and temperatures on germination were investigated. At the end of these experiments, a final protocol was determined for the in vitro germination of D. hastata seeds. With this protocol, the highest germination rate was obtained with distilled water supplemented with vitamins and 1 mg L^{-1} of gibberellic acid (GA_{3}), with a 16:8 photoperiod at 24 ± 2 …

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Towards Cloning A Tyrosinase Gene From Pitayas, Kurt J. Chamberlain Jan 2010

Towards Cloning A Tyrosinase Gene From Pitayas, Kurt J. Chamberlain

Honors Program Theses

Betalains, such as those produced by pitayas, are water-soluble nitrogen-containing pigment molecules (Gandia-Herrero et al., 2005). In most families of the Caryophyllales, betalains replace anthocyanins, which are the pigment molecules found in all other families of flowering plants (Strack et al., 2001). Betalains accumulate in the flowers, fruits, and occasionally vegetative tissues of these plants (Strack et al., 2001). Depending on the structure of the molecule, betalains are classified as either betacyanins or betaxanthins. Betacyanins contribute to red-violet coloration, while betaxanthins contribute to the yellow-orange coloration found only in a limited number of plant species (Gandia-Herrero et al., 2005). The …

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A Comparison Of Soil And Corn Kernel Aspergillus Flavus Populations: Evidence For Niche Specialization, Rebecca Ruth Sweany Jan 2010

A Comparison Of Soil And Corn Kernel Aspergillus Flavus Populations: Evidence For Niche Specialization, Rebecca Ruth Sweany

LSU Master's Theses

Aspergillus flavus is an opportunistic fungal pathogen that infects peanuts, cotton, corn and tree nuts. Aspergillus flavus is a major problem globally due to the production of acutely toxic and carcinogenic aflatoxins. Louisiana climatic conditions lead to annual threats of corn aflatoxin contamination. The purpose of this study was to determine the specific ability of different strains of A. flavus to infect corn. Five soil samples and 10 corn ears were collected from each of seven corn fields throughout Louisiana. In addition, Francis Deville of Monsanto Company collected 7, 6, 2, and 4 soil samples and corn ears from four …

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Symptoms, Distribution And Abundance Of The Stem-Boring Caterpillar, Blastobasis Repartella (Dietz), In Switchgrass, Jarrad R. Prasifka, Jeffrey D. Bradshaw, Arvid A. Boe, Dokyoung Lee, David Adamski, Michael E. Gray Jan 2010

Symptoms, Distribution And Abundance Of The Stem-Boring Caterpillar, Blastobasis Repartella (Dietz), In Switchgrass, Jarrad R. Prasifka, Jeffrey D. Bradshaw, Arvid A. Boe, Dokyoung Lee, David Adamski, Michael E. Gray

Agronomy, Horticulture and Plant Science Faculty Publications

A potential pest of switchgrass, Panicum virgatum L., was first detected in South Dakota in 2004, where death of partially emerged leaves was noted in a small proportion of tillers. Similar “dead heart” symptoms were observed in switchgrass in Illinois during 2008 and adults of a stem-boring caterpillar were collected and identified as Blastobasis repartella (Dietz). In 2009, a survey of the central United States was used to estimate the distribution and abundance of this insect. In eight northern states, B. repartella was consistently found in both cultivated plots and natural stands of switchgrass. In four southern states, B. repartella …

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Increased Weed Diversity, Density And Above-Ground Biomass In Long-Term Organic Crop Rotations, Samuel E. Wortman, John L. Lindquist, Milton J. Haar, Charles A. Francis Jan 2010

Increased Weed Diversity, Density And Above-Ground Biomass In Long-Term Organic Crop Rotations, Samuel E. Wortman, John L. Lindquist, Milton J. Haar, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

While weed management is consistently a top priority among farmers, there is also growing concern for the conservation of biodiversity. Maintaining diverse weed communities below bioeconomic thresholds may provide ecosystem services for the crop and the surrounding ecosystem. This study was conducted to determine if weed diversity, density and biomass differ within and among organic and conventional crop rotations. In 2007 and 2008, we sampled weed communities in four long-term crop rotations near Mead, Nebraska, United States using seedbank analyses (elutriation and greenhouse emergence) and above-ground biomass sampling. Two conventional crop rotations consisted of a corn (Zea mays) …

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Molecular Studies Of 5-Azacytidine-Induced Early-Flowering Lines Of Flax, Megan A. House Jan 2010

Molecular Studies Of 5-Azacytidine-Induced Early-Flowering Lines Of Flax, Megan A. House

Theses and Dissertations (Comprehensive)

Several early-flowering flax (Linum usitatissimum L.) lines were derived from treatment of germinating seeds with 5-azacytidine in 1990. These lines are also shorter, have fewer leaves, and their DNA is hypomethylated, relative to their corresponding controls. The work presented in this thesis used early-flowering and control lines of the Royal (R) flax genotype, and the Large (L) flax genotroph. Firstly, levels of cytosine methylation were measured over a 24-hour period in the early-flowering line RE2 and its control (RC), using an HPLC method. Secondly, to determine the response of the flax lines to short-day conditions, control and early-flowering …

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Cover Crops And Tillage Systems For Organic Corn Production In Kentucky, Alfonso Suarez Jan 2010

Cover Crops And Tillage Systems For Organic Corn Production In Kentucky, Alfonso Suarez

University of Kentucky Master's Theses

Organic corn (Zea mays L.) producers generally use intensive tillage for weed control. No-till methods reduce soil erosion, conserve water, maintain soil structure and reduce CO2 emissions. The objective of this study was test different cover crops, tillage systems, N sources and N rates for organic corn production. Two tillage systems (no-till and moldboard plow), two cover crops [hairy vetch (Vicia villosa) and rye (Secale cereale)] and two organic N sources [Louisville Green (LG) and Nature Safe (NF)] at four N rates (45, 90, 135, 180 kg N ha-1) were evaluated …

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Chromium, Copper, And Arsenic Concentration And Speciation In Soil Adjacent To Chromated Copper Arsenate (Cca) Treated Lumber Along A Topohydrosequence, Donald Roy Schwer Iii Jan 2010

Chromium, Copper, And Arsenic Concentration And Speciation In Soil Adjacent To Chromated Copper Arsenate (Cca) Treated Lumber Along A Topohydrosequence, Donald Roy Schwer Iii

University of Kentucky Master's Theses

Arsenic (As), Chromium (Cr), and Copper (Cu) are ubiquitous in soils as a result of anthropogenic and geogenic processes. The fate of As, Cr, and Cu in the environment is largely governed by their speciation, which is influenced by soil physiochemical properties. This study investigated the influence of soil physiochemical properties and landscape position on As, Cr, and Cu concentration and speciation in soils adjacent to Chromated Copper Arsenate (CCA) treated lumber fence posts. Concentration gradients showed elevated total As and Cu adjacent to the three fence posts, which decreased with increasing distance from the posts. In addition, As and …

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Low Co-Cultivation Temperature At 20oc Improved Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco Leaf Disks, Guiying Su Jan 2010

Low Co-Cultivation Temperature At 20oc Improved Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco Leaf Disks, Guiying Su

LSU Master's Theses

I investigated the effect of co-cultivation temperature and binary Ti vectors on the fresh weight yield of leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith) after Agrobacterium tumefaciens-mediated transformation. I concluded that the temperature at 20ºC during co-cultivation of leaf disks with A. tumefaciens was the most critical factor on the reproducible enhancement of fresh weight yield. The highest fresh weight increase and presumably transformation efficiency was achieved under the following experimental conditions: A. tumefaciens strain LBA4404 containing pLSU binary Ti vector was grown overnight at 28oC in liquid Agrobacterium medium and the concentration was adjusted to …

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Study Of Host-Fungus Interactions Between Soybean And Phakopsora Pachyrhizi Using Proteomics, Sunjung Park Jan 2010

Study Of Host-Fungus Interactions Between Soybean And Phakopsora Pachyrhizi Using Proteomics, Sunjung Park

LSU Doctoral Dissertations

Asian soybean rust, caused by Phakopsora pachyrhizi, is an emerging disease in the continental U. S. and resistant commercial varieties have not been reported. In an effort to understand the interactions during rust infection of soybean, protein profile changes were examined over a 14-day period in soybean leaves of one susceptible commercial line (Pioneer 93M60) with or without soybean rust inoculation using proteomics in this study. Forty protein spots differentially expressed after rust inoculation were identified and fourteen of them were recovered and sequenced. These included proteins involved in plant defense, stress, metabolism, and other biological processes. During the time-course …

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Improvement Of New Oil Crops For Kentucky, Watchareewan Jamboonsri Jan 2010

Improvement Of New Oil Crops For Kentucky, Watchareewan Jamboonsri

University of Kentucky Doctoral Dissertations

Three oil crops, chia (Salvia hispanica L.), flax (Linum usitatissimum L.), and castor (Ricinus communis L.), were studied because of their nutritional and industrial values. Chia and flax are rich in an ω3 fatty acid, α-linolenic acid, and castor is a very high oil producer and high in a hydroxy fatty acid. Ethyl methanesulfonate (EMS) and gamma rays were employed to mutagenize chia seeds to produce early flowering mutants. The M1 population was grown and induced to flower by short-day photoperiods. The M2 population was planted in the field in Lexington, KY in 2008. Early …

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Insights Into The World Of Pea Nodulation Using The Low Nodulator R50, Scott Clemow Jan 2010

Insights Into The World Of Pea Nodulation Using The Low Nodulator R50, Scott Clemow

Theses and Dissertations (Comprehensive)

Cytokinin oxidase (CKX) is the enzyme responsible for the degradation of cytokinin, a class of adenine-based plant hormones that stimulate cell division, among other physiological processes. The pea mutant R50 is characterized by having a pale leaf phenotype, dwarf stature, few lateral roots, low nodule formation, and elevated levels of endogenous cytokinins in its shoots, roots and nodules. When compared to that of the wild-type Sparkle, total CKX activity is low but the transcript levels of PsCKX1 are significantly higher. In this study, I investigated the expression of PsCKX1 throughout the development of a nodule and the localization of PsCKX1 …

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The Pleiotropic Mutant R50 (Sym16): A Link Between Cytokinin And Starch Metabolism May Explain Its Seed Phenotype, Chengli Long Jan 2010

The Pleiotropic Mutant R50 (Sym16): A Link Between Cytokinin And Starch Metabolism May Explain Its Seed Phenotype, Chengli Long

Theses and Dissertations (Comprehensive)

Cytokinins are a class of adenine-based plant hormones that stimulate cell divisions and are therefore essential for organ growth and development. The role that cytokinin plays in nodulation and that of R50 (syml6) in particular is very complex. In this study, a cytokinin antagonist and a cytokinin oxidase (CKX) inhibitor were used as tools to study the effects of cytokinin perception or cytokinin degradation on nodulation in pea, the cytokinin transduction pathway, especially that induced by CRE1/AHK4, and CKX play a significant role in pea nodulation. Comparison of the full length sequences PsCKX2 cDNAs from Sparkle and R50 …

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Don't Judge A Book By Its Cover: The Curious Case Of Wild Ginger Pollination, W. John Hayden Jan 2010

Don't Judge A Book By Its Cover: The Curious Case Of Wild Ginger Pollination, W. John Hayden

Biology Faculty Publications

What pollinates wild ginger? This seems like an easy question. The inconspicuous little flowers are held close to the forest floor, often completely hidden by a dense canopy of ginger leaves above. Flower color is rather drab, dominated by brown and maroon hues. Wind pollination seems completely unlikely and flowers pollinated by bees, butterflies, moths, or hummingbirds are always much more showy and accessible to these flying creatures. Flies, however, given their natural inclination to seek carrion as a food source for their babies (i.e. maggots), are often attracted to brown and maroon flowers. And because their actual quarry, animal …

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Morphogenesis And Tissue Culture Studies Of Gerbera Jamesonii Bolus Ex. Hook F., Nor Azlina Hasbullah Jan 2010

Morphogenesis And Tissue Culture Studies Of Gerbera Jamesonii Bolus Ex. Hook F., Nor Azlina Hasbullah

Student Works (2010-2019)

In vitro regeneration of Gerbera jamesonii Bolus ex. Hook f. was successfully carried out in this study. Petiole and leaf explants from 8-week-old aseptic seedlings were used as source of explants. Different response was observed when different explants were cultured on the culture media. MS (Murashige and Skoog, 1962) supplemented with 2.0 mg/l BAP and 0.5 mg/l NAA was identified as the optimum medium for shoot regeneration, while petiole explant was identified as the most responsive explant in this study. Petiole explants regenerated shoots (94.3 } 2.5 %) and produced 9.3 } 0.6 shoots per explant. MS basal medium was …

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Studies Of Antioxidant And Angiotensin Converting Enzyme Inhibitory Activity Of Orthosiphon Stamineus (Misai Kucing)., Che Wan Imanina Che Wan Takwa Jan 2010

Studies Of Antioxidant And Angiotensin Converting Enzyme Inhibitory Activity Of Orthosiphon Stamineus (Misai Kucing)., Che Wan Imanina Che Wan Takwa

Student Works (2010-2019)

Abstract NA

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Physiological And Biochemical Attributes Of Red-Fleshed Dragon Fruit, Hylocereus Polyrhizus., Punitha R. Vasanthan Jan 2010

Physiological And Biochemical Attributes Of Red-Fleshed Dragon Fruit, Hylocereus Polyrhizus., Punitha R. Vasanthan

Student Works (2010-2019)

An understanding of the postharvest parameters is essential for the successful market introduction of red-fleshed dragon fruit, Hylocereus polyrhizus, which has been recently developed as fruit crop. In this study, fruit at three different harvest maturities were evaluated in terms of physiological and biochemical aspects. Fruit which were harvested at complete red stage maintained their quality for up to one week. On the other hand, fruit harvested at early maturity attained the physical properties of fruit ripened on the plant (firmness reduced, peel colour changes, pH increased) but they contained noticeably lower concentrations of soluble sugars suggesting that fruit can …

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Marine Algae As A Source Of Photosensitisers For Photodynamic Therapy Of Cancer., Tang Yee Voon Jan 2010

Marine Algae As A Source Of Photosensitisers For Photodynamic Therapy Of Cancer., Tang Yee Voon

Student Works (2010-2019)

Photodynamic therapy (PDT) involves the intravenous administration of a photosensitiser that preferentially localises in cancerous tissues more than the surrounding normal tissues. Subsequent illumination of photosensitiser generates reactive oxygen species that cause irreversible photo-damage to the cancer cells. To date, most photosensitisers that have been approved for clinical use or are currently in clinical trials are cyclic tetrapyrroles which are derived or inspired by naturally occurring chlorophyll and its degradation products. Some non-tetrapyrrolic photosensitisers have also been found in nature suggesting that there are potential photosensitisers with interesting structures in nature. To this end, this thesis outlines the collection of …

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Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song Jan 2010

Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song

Student Works (2010-2019)

Two candidate yield-related genes, putative Receptor-like Protein Kinase 1 (putative RPK1) and putative CLAVATA1 Receptor Kinase (putative CLV1), were selected for molecular characterization and expression analysis. This was carried out using in four rice lines comprising the parents and two BC2F7 lines from a local rice breeding program, with Malaysian rice variety, Oryza sativa ssp. indica cv. MR219 as recurrent parent, and Oryza rufipogon (IRGC105491) as donor parent. Approximately 2.8 kb and 2.6 kb putative RPK1 cDNA nucleotide sequences were obtained from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219 respectively. However, only 402 …

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Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin Jan 2010

Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin

Student Works (2010-2019)

This study was aimed to investigate the effects of physiological stresses such as pruning, shading, phloemic stress and plant hormones such as gibberellic acid (GA3) and naphthalene acetic acid (NAA) on the growth, development and flowering process of bougainvillea plants. The plants (Bougainvillea glabra and Bougainvillea spectabilis) were grown under prevailing conditions (temperature 21-32°C, maximum PAR 2100 μE m-2 s-1 and relative humidity of 60-90%) using completely randomized design. Field experiments as well as biochemical analysis were carried out at the Plant Physiology Garden and the Plant Physiology Laboratory, Institute of Biological Sciences, University of Malaya. Results on frequent pruning …

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Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan Jan 2010

Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan

Student Works (2010-2019)

Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has been shown to be an important regulator of the AP2 family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved miRNA that can be identified based on sequence homology. Six oil palm miR172 paralogs, representing two distinct mature miR172 sequences, …

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Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification., Tnah Lee Hong Jan 2010

Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification., Tnah Lee Hong

Student Works (2010-2019)

Tectonic movement and climatic oscillations during the Cenozoic have had dramatic effect on the biota of the tropical rain forest. This study aims to reveal the phylogeography and evolutionary history of a Peninsular Malaysian endemic tropical timber, Neobalanocarpus heimii (Dipterocarpaceae), based on chloroplast DNA (cpDNA) variation. Fifteen haplotypes were identified from 10 intraspecific variable sites of five non-coding cpDNA regions: trnL intron, trnS-trnG spacer, trnG intron, trnK intron and psbK-trnS spacer. Two major genealogical cpDNA lineages of N. heimii were elucidated: a widespread southern and a northern region. The species is predicted to survive in multiple refugia during climatic oscillation: …

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Studies Of Antioxidant Activity Of Bioactive Compounds Derived From Ardisia Crispa., Hassan Mahmood Kzar Jindal Jan 2010

Studies Of Antioxidant Activity Of Bioactive Compounds Derived From Ardisia Crispa., Hassan Mahmood Kzar Jindal

Student Works (2010-2019)

In recent times, researches have focused on medicinal plants in order to extract natural and low cost antioxidants that can help protect the human body against oxidative stress, and replace the synthetic additives that might be carcinogenic. In present study, the crude extracts of leaves and fruits from Ardisia crispa were investigated for their chemical constituents and antioxidant activity. Preliminary phytochemical screening of the crude extracts via TLC analysis revealed the presence of several secondary compounds mainly, terpenoids and phenolic compounds in addition to alkaloids and saponins. HPLC analysis showed the possibility of presence of gallic acid in the fruits …

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Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari Jan 2010

Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari

Student Works (2010-2019)

The selectable marker system based on the Escherichia coli phosphomannose isomerase (pmi) gene was adapted for genetic transformation of oil palm. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate and uses mannose as the selection agent. This is to anticipate the future requirement of using non-antibiotic resistance genes for the commercialization of transgenic oil palm. The use of antibiotic or herbicide-based selection systems has caused much public concern due to inadequate knowledge of the agents’ impact on the environment and on human health. In this study, four transformation vectors, namely pMI3, …

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Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah Jan 2010

Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah

Student Works (2010-2019)

The aim of this study was to isolate protein/peptide and secondary metabolites from plant and fermented sources. The extracts were used to test for antimicrobial and antioxidant properties. In the current study protein/peptide extracts of Allium sativum showed promising results with a 15mm inhibition zone against Escherichia coli, 28mm against Staphylococcus aureus, 16.3mm against Bacillus cereus and 9mm against Pseudomonas aeruginosa. The minimum bacteriocidal concentration (MBC) results showed that Allium sativum protein/peptide extract is only bacteriostatic and not bacteriocidal. Andrographis paniculata showed the most promising result for the ethanolic extracts with an inhibition zone of 14mm against B. cereus, 11.5mm …

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A Systematic Study Of Fagraea Sensu Lato And A Revision Of The Peninsular Malaysian Taxa., Sugumaran Manickam Jan 2010

A Systematic Study Of Fagraea Sensu Lato And A Revision Of The Peninsular Malaysian Taxa., Sugumaran Manickam

Student Works (2010-2019)

systematic study of the Peninsular Malaysian species of Fagraea sensu lato was undertaken. This was done in the light of recent revisionary work done for Borneo that documented 20 new species and demonstrated that previous species concepts for Fagraea were too broad. Parallel to this the distinction of the subgeneric groups recognised as sections, i.e., Cyrtophyllum, Fagraea and Racemosae, were investigated with molecular phylogenetic methods. Representative taxa from the Malay Peninsula and Borneo augmented by sequences from other taxa in the same subtribe and tribe were used in the molecular analysis. Gene sequences from ITS, trnL–F (trnL intron + trnL–F …

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