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Nebline, October 2010

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Can Help Youth Choose a Career Path

Farm Views

Horticulture

Food & Fitness

Home & Family Living

Environmental Focus

Urban Agriculture

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Mojave Applied Ecology Notes Fall 2010, Adria Decorte, Sharon Altman, Alex Suazo

Mojave Applied Ecology Notes

Adria DeCorte defense, Mojave desert inventory and monitoring mapping project, effect of burial depth on Brassica tournefortii seeds

Effects Of Experimental Nitrogen Additions On Plant Diversity In An Old-Growth Tropical Forest, Xiankai Lu, Jiangming Mo, Frank S. Gilliam, Guoyi Zhou, Yunting Fang

Biological Sciences Faculty Research

Response of plant biodiversity to increased availability of nitrogen (N) has been investigated in temperate and boreal forests, which are typically N-limited, but little is known in tropical forests. We examined the effects of artificial N additions on plant diversity (species richness, density and cover) of the understory layer in an N saturated old-growth tropical forest in southern China to test the following hypothesis: N additions decrease plant diversity in N saturated tropical forests primarily from N-mediated changes in soil properties. Experimental additions of N were administered at the following levels from July 2003 to July 2008: no addition (Control); …

Traditional Plant Use Of The Raglay In Cãu Gãy Village, Núi Chúa National Park, Alex Greene

Independent Study Project (ISP) Collection

Members of the Raglay community of Cãu Gãy Village were interviewed to determine the extent and nature of their traditional reliance on plants. This community, located in the buffer zone of Núi Chúa National Park, was found to utilize 64 plant species for a wide variety of uses. Botanical specimens and photographs were used to identify 42 plants to species level, 13 to genus level, and 6 to family level, while 3 remained unidentified. For each plant, the Raglay name, local Vietnamese name, use, specific application, and preparation were documented, as well as any details of ritual or commercial significance. …

My 21st Century Expedition: Following The Route Of Schoolcraft 1820, 1832 To The Source Of The Mississippi River, Lorah Patterson

Honors Theses

The journal of Lorah Patterson during her expedition from Schoolcraft, Michigan, to the source of the Mississippi River. Supplemental file contains complete Herbarium. The thesis includes only a few examples.

Growing South Dakota (Fall 2010), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Focused on Lean Growth: Despite Challenging Times, Dean Barry Dunn Has a Vision of Growth and Excellence for ABS
[Page] 6 Building Better Communities: Horizons Helps Small Town South Dakota
[Page] 8 On Campus
[Page] 9 Urban Opportunities: A New Twist to Attract Urban Youth
[Page] 10 Saddle Up: Steers for State Helps SDSU Rodeo Program Attain Success [Page] 12 Inspiring Students: This Professor's Teaching & Research Style Is Earning Awards and Student Accolades
[Page] 14 Fish Feed :The Newest Market for South Dakota Crops 15 College News [Page] 16 Field Notes from Lyman County: Bob Fanning's Experience …

Joint Fire Science Program – Lake Mead National Recreation Area Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis: Quarterly Progress Report, Time Period: July 1 — September 30, 2010, Margaret N. Rees

Fire Science

• Article entitled “Competitive Hierarchy of Native Desert Plants with Red Brome (Bromus rubens): Towards Identifying Invasion-Reducing Species" was revised and re-submitted to the Invasive Plant Science and Management journal.
• Final data collection on soils and nursery plots is complete. Data are awaiting analysis.

Systematic Analysis Of Nutritional Components, Cell Wall Chemical Features And Potential Pathway(S) Of Assimilate Transport In The Developing Pods And Seeds Of Medicago Truncatula, Alka Sharma

Theses and Dissertations

Medicago truncatula is a model species for legume studies. Despite the great advances in the study of M. truncatula and development of a vast array of genetic tools and genomic resources, there is a lack of knowledge regarding the nutrient movement and accumulation in its pods and seeds. This study used a combination of the microscopic analysis, histochemical localization and the fluorescent tracers to identify the cellular course and location of nutrient movement. Temporal histochemical localization revealed that the longer duration of the accumulation of protein bodies and lipid droplets combined with the high hexoses concentration in seed apoplasm might …

Empirical Geographic Modeling Of Switchgrass Yields In The United States, Henriette I. Jager, Latha M. Baskaran, Craig C. Brandt, Ethan B. Davis

Dartmouth Scholarship

Switchgrass (Panicum virgatum L.) is a perennial grass native to the United States that has been studied as a sustainable source of biomass fuel. Although many field‐scale studies have examined the potential of this grass as a bioenergy crop, these studies have not been integrated. In this study, we present an empirical model for switchgrass yield and use this model to predict yield for the conterminous United States. We added environmental covariates to assembled yield data from field trials based on geographic location. We developed empirical models based on these data. The resulting empirical models, which account for spatial …

Plant Immunity Directly Or Indirectly Restricts The Injection Of Type Iii Effectors By The Pseudomonas Syringae Type Iii Secretion System, Emerson Crabill, Anna Joe, Anna Block, Jennifer M. Van Rooyen, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Plants perceive microorganisms by recognizing microbial molecules known as pathogen-associated molecular patterns (PAMPs) inducing PAMP-triggered immunity (PTI) or by recognizing pathogen effectors inducing effector-triggered immunity (ETI). The hypersensitive response (HR), a programmed cell death response associated with ETI, is known to be inhibited by PTI. Here, we show that PTI-induced HR inhibition is due to direct or indirect restriction of the type III protein secretion system’s (T3SS) ability to inject type III effectors (T3Es). We found that the Pseudomonas syringae T3SS was restricted in its ability to inject a T3E-adenylate cyclase (CyaA) injection reporter into PTI-induced tobacco (Nicotiana tabacum) cells. …

The Chlorella Variabilis Nc64a Genome Reveals Adaptation To Photosymbiosis, Coevolution With Viruses, And Cryptic Sex, Guillaume Blanc, Garry Duncan, Irina Agarkova, Mark Borodovsky, James Gurnon, Alan Kuo, Erika Lindquist, Susan Lucas, Jasmyn Pangilinan, Juergen Polle, Asaf Salamov, Astrid Terry, Takashi Yamada, David D. Dunigan, Igor V. Grigoriev, Jean-Michel Claverie, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Chlorella variabilis NC64A, a unicellular photosynthetic green alga (Trebouxiophyceae), is an intracellular photobiont of Paramecium bursaria and a model system for studying virus/algal interactions. We sequenced its 46-Mb nuclear genome, revealing an expansion of protein families that could have participated in adaptation to symbiosis. NC64A exhibits variations in GC content across its genome that correlate with global expression level, average intron size, and codon usage bias. Although Chlorella species have been assumed to be asexual and nonmotile, the NC64A genome encodes all the known meiosis-specific proteins and a subset of proteins found in flagella. We hypothesize that Chlorella might have …

Nebline, September 2010

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Wildlife Rescue Team Helps Orphaned and Injured Wildlife

Farm Views

Horticulture

Food & Fitness

Home & Family Living

Environmental Focus

Urban Agriculture

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Ability Of Plectranthus Spp. To Re-Green After Nitrogen Deficiency, Jazbaat K. Chahal

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is the most important nutrient for plant growth and development. This is due to the reason that nitrogen is a key constituent of amino acids, nucleic acids, lipids and chlorophyll. Stress caused by nitrogen deficiency has a large impact on plant growth and development. In most plants, older leaves become chlorotic and eventually fall off if nitrogen stress is not relieved. Chlorophyll concentration is important to determine the nitrogen concentration in a plant as nitrogen is a constituent element of chlorophyll and also of various proteins that are a part of the photosynthetic apparatus. Cytokinins are said to mediate …

Summaries Of Arkansas Cotton Research 2009, Derrick M. Oosterhuis

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Increased Plant Uptake Of Nitrogen From 15N-Depleted Fertilizer Using Plant Growth-Promoting Rhizobacteria, A. O. Adesemoye, H. A. Torbert, J. W. Kloepper

Department of Plant Pathology: Faculty Publications

Harmful environmental effects resulting from fertilizer use have spurred research into integrated nutrient management strategies which can include the use of specific microorganisms to enhance nutrient use efficiency by plants. Some strains of plant growth-promoting rhizobacteria (PGPR) have been reported to enhance nutrient uptake by plants, but no studies with PGPR have used ¹⁵N isotope techniques to prove that the increased N in plant tissues came from the N applied as fertilizer. The current study was conducted to demonstrate that a model PGPR system can enhance plant uptake of fertilizer N applied to the soil using different rates of …

Turfgrass Cultural Practices And Insect Pest Management, Diane Alston, Kelly Kopp

All Current Publications

There are a number of insects that can cause aesthetic and economic loss to turfgrass in Utah – in home lawns as well as in athletic fields and on recreational lands. Good turfgrass cultural practices are the primary way to prevent insect infestation and turfgrass damage.

Role Of Sabp2 In Systemic Acquired Resistance Induced By Acibenzolar-S-Methyl In Plants., Diwaker Tripathi

Electronic Theses and Dissertations

Plants have evolved an efficient mechanism to defend themselves against pathogens. Many biotic and abiotic agents have been shown to induce defense mechanism in plants. Acibenzolar-S-Methyl (ASM) is a commercially available chemical inducer of local and systemic resistance (SAR) response in plants. ASM functioning at molecular level is mostly unclear. This research was designed to investigate the mechanism of ASM action in plants. It was hypothesized that SABP2, a plant protein, plays an important role in ASM-mediated defense signaling. Biochemical studies were performed to test the interaction between SABP2 and ASM. Transgenic SABP2-silenced tobacco plants were used to determine the …

The Endosymbiotic Relationship Of Leguminosae (Fabaceae) And Rhizobium, Sabrina Mcgee, Dolores Maceda, Maria Bustos, Maryknoll Palisoc, Rachel Dahl

Undergraduate Research Opportunities Program (UROP)

This project will look at The Endosymbiotic Relationship of Leguminosae (Fabaceae) and Rizobioum. Endoysmbiosis Theory is the concept that mitochondria and chloroplasts are the result of years of evolution initiated by the endocytosis, (the process by which cells absorb material through their cell membrane) of bacteria and algae which, instead of becoming digested, became symbiotic.The mitochondria of eukaryotes evolved from aerobic bacteria, probably rickettsias, living within their host cell and the chloroplasts from cyanobacteria, also known as blue-green algae. Endoysmbiosis is a type of symbiosis in which one organism lives inside the body of another and both function as a …

Reestablishing Diversity In Our Hardwood Forests: A Transplant Study Of Five Spring-Flowering Herbs, Danielle Racke

Masters Theses & Specialist Projects

Herbaceous communities are critical to the functioning of forest ecosystems. They recycle nutrients, help prevent erosion, provide critical microhabitats and maintain biodiversity. In the eastern United States, most hardwood forests are growing on land once entirely cleared or used for some form of agriculture. Although some of these forests are nearly 150 years old, they still have depauperate native herbaceous communities when compared to remaining old-growth forests. This long-term depletion may result from dispersal limitation or environmental limitation.

I tested the hypothesis that dispersal was the primary factor contributing to the absence of five spring-flowering herbaceous species in four secondary …

Genetic Mapping Of Quantitative Trait Loci Associated With Bioenergy Traits, And The Assessment Of Genetic Variability In Sweet Sorghum (Sorghum Bicolor (L.). Moench), Aatshwaelwe Lekgari Lekgari

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sweet sorghum, a botanical variety of sorghum is a potential source of bioenergy because high sugar levels accumulate in its stalks. The objectives of this study were to explore the global diversity of sweet sorghum germplasm, and map the genomic regions that are associated with bioenergy traits. In assessing diversity, 142 sweet sorghum accessions were evaluated with three marker types (SSR, SRAP, and morphological markers) to determine the degree of relatedness among the accessions. The traits measured (anthesis date [AD], plant height [PH], biomass yield [BY], and moisture content [MC]) were all significantly different (P< 0.05) among accessions. Morphological marker clustered the accessions into five groups based on PH, MC and AD. The three traits accounted for 92.5% of the variation. There were four and five groups based on SRAP and SSR data respectively classifying accessions mainly on their origin or breeding history. The observed difference between SSR and SRAP based clusters could be attributed to the difference in marker type. SSRs amplify any region of the genome whereas SRAP amplify the open reading frames and promoter regions. Comparing the three marker-type clusters, the markers complimented each other in grouping accessions and would be valuable in assisting breeders to select appropriate lines for crossing. In evaluating QTLs that are associated with bioenergy traits, 165 recombinant inbred lines (RILs) were planted at four environments in Nebraska. A genetic linkage map constructed spanned a length of 1541.3 cM, and generated 18 linkage groups that aligned to the 10 sorghum chromosomes. Fourteen QTLs (6 for brix, 3 for BY, 2 each for AD and MC, and 1 for PH) were mapped. QTLs for the traits that were significantly correlated, colocalized in two clusters on linkage group Sbi01b. Both parents contributed beneficial alleles for most of traits measured, supporting the transgressive segregation in this population. Additional work is needed on exploiting the usefulness of chromosome 1 in breeding sorghum for bioenergy.

Temporal And Spatial Assessment Of Evaporation, Transpiration, And Soil Moisture Redistribution, Brian M. Bird

UNLV Theses, Dissertations, Professional Papers, and Capstones

At a native stand of creosote bush (Larrea tridentata) in North Las Vegas, a rainfall simulation study was conducted over a 12 month period from October 2005 to October 2006. Simulated rainfall occurred during the winter, spring, summer, and fall periods. Rainfall simulation systems were positioned on each of 12 plots, each containing a single creosote bush. Simulated rainfall events occurred at night with multiple short pulses designed to maximize infiltration while minimizing ponding. Yearly simulated rainfall amounts were set at 0, 15, 30 and 60 cm (replicated three times) and were approximately 0, 1.5, 3.0 and 6.0 times the …

Unique Functionality Of 22 Nt Mirnas In Triggering Rdr6-Dependent Sirna Biogenesis From Target Transcripts In Arabidopsis, Josh T. Cuperus, Alberto Carbonell, Noah Fahlgren, Hernan Garcia-Ruiz, Russell T. Burke, Atsushi Takeda, Christopher M. Sullivan, Sunny D. Gilbert, Taiowa A. Montgomery, James C. Carrington

Department of Plant Pathology: Faculty Publications

RNA interference pathways may involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNA (miRNA). Here, Arabidopsis thaliana secondary siRNA from mRNA, and trans-acting siRNA, are shown to be triggered through initial targeting by 22 nt miRNA that associate with AGO1. In contrast to canonical 21 nt miRNA, 22 nt miRNA primarily arise from foldback precursors containing asymmetric bulges. Using artificial miRNA constructs, conversion of asymmetric foldbacks to symmetric foldbacks resulted in production of 21 nt forms of miR173, miR472 and miR828. Both 21 and 22 nt forms associated …

Heterokaryotic Nuclear Conditions And A Heterogeneous Nuclear Population Are Observed By Flow Cytometry In Phytophthora Infestans, Mursel Catal, Louis King, Pavani Tumbalam, Prissana Wiriyajitsomboon, William W. Kirk, Gerard C. Adams

Department of Plant Pathology: Faculty Publications

A simple and reliable method for preparation of whole nuclei of a common oomycete, Phytophthora infestans, is described for laser flow cytometry. The ease of preparation, the absence of detectable debris and aggregates, and the precision in determinations of DNA content per nucleus improve interpretation and understanding of the genetics of the organism. Phytophthora infestans is the pathogen that causes potato and tomato late blight. The genetic flexibility of P. infestans and other oomycete pathogens has complicated understanding of the mechanisms of variation contributing to shifts in race structure and virulence profiles on important agricultural crops. Significant phenotypic and …

B. R. Wells Rice Research Studies 2009, R. J. Norman, K. A. K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

A New Species Of Calceolaria (Calceolariaceae) From Disturbed Paramos In South Ecuador, Pamela Puppo

Biological Sciences Faculty Research

Calceolaria molaui Puppo, sp. nov. is described and illustrated. The new species was collected in Loja, in disturbed paramos near the frontier with Peru so it would be expected to occur in northern Peru as well. It is characteristic for having hirsute indumentum composed of branched trichomes, ovate, subcoriaceous leaves with reticulate venation, cyme bracts absent, elaiophore absent, and brown, deflexed anthers, totally dehiscent. Due to its indumentum, leave shape, and anther morphology, C. molaui is placed in Section Lehmannina Pennell within subgenus Calceolaria. This is the only species of this section in southern Ecuador.

Plasticity In Cavitation Resistance And Mechanical Strength Of Stem Xylem In Heteromeles Arbutifolia, Adults Versus Post-Fire Resprouts, Iolana N. Kaneakua, B. A. Bergman, F. W. Ewers, S. D. Davis

Biology

Background/Questions/Methods

Many species of chaparral shrubs of California undergo vigorous resprout success after wildfire. We hypothesized that the water transport properties of resprouts, as well as their biomechanical strength would differ from adults. In addition, we hypothesized that irrigated and shaded resprouts would display reduced cavitation resistance and biomechanical strength in comparison to controls and adults. We tested these hypotheses by using a centrifuge method to compare the vulnerability of xylem to water stress induced cavitation. We used 50% loss in hydraulic conductivity due to water stress (PLC₅₀) as our estimate of vulnerability to cavitation. A Universal Materials …

Spatial Heterogeneity Of Soil Nutrients And Plant Species In Herb-Dominated Communities Of Contrasting Land Use, Frank S. Gilliam, David A. Dick

Biological Sciences Faculty Research

Recent interest in spatial pattern in terrestrial ecosystems has come from an awareness of theintimate relationship between spatial heterogeneity of soil resources and maintenance of plant species diversity. Soil and vegetation can vary spatially inresponse to several state factors of the system. In this study, we examined fine-scale spatial variability of soil nutrients and vascular plant species in contrasting herb-dominated communities (a pasture and an oldfield) to determine degree of spatial dependenceamong soil variables and plant community characteristics within these communities by sampling at 1-m intervals. Each site was divided into 25 1-m 2 plots. Mineral soil was sampled (2-cm …

Alteration In Lignin Biosynthesis Restricts Growth Of Fusarium Spp. In Brown Midrib Sorghum, Deanna L. Funnell-Harris, Jeffrey F. Pedersen, Scott E. Sattler

Department of Plant Pathology: Faculty Publications

To improve sorghum for bioenergy and forage uses, brown midrib (bmr)6 and -12 near-isogenic genotypes were developed in different sorghum backgrounds. The bmr6 and bmr12 grain had significantly reduced colonization by members of the Gibberella fujikuroi species complex compared with the wild type, as detected on two semiselective media. Fusarium spp. were identified using sequence analysis of a portion of the translation elongation factor (TEF) 1-α gene. The pathogens Fusarium thapsinum, F. proliferatum, and F. verticillioides, G. fujikuroi members, were commonly recovered. Other frequently isolated Fusarium spp. likely colonize sorghum asymptomatically. The χ2 analyses showed that the …

Determining The Fate Of Hybridized Genomes In The Allopolyploid Brassica Napus, Tina Y. Wang

Master's Theses

Polyploidy is widely acknowledged as a widespread mechanism in the evolution and speciation of the majority of flowering plants. Allopolyploid forms through interspecific hybridization and whole genome duplication. While allopolyploids may display increased vigor relative to their progenitors, they can also face challenges to fertility following hybridization. Genetic changes in allopolyploids result from recombination between the hybridized subgenomes, which can influence phenotype and ultimately determine fitness of future generations. To study dynamic changes that follow allopolyploid formation, Brassica napus lineages were derived by hybridizing Brassica oleracea and Brassica rapa. Two lineages of B. napus were analyzed for genetic and phenotypic …

Mojave Applied Ecology Notes Summer 2010, Scott R. Abella, E. Cayenne Engel

Mojave Applied Ecology Notes

Survey of monitoring and management for conservation of rare plants, Roadside restoration techniques in Joshua Tree NP, and an update on renewable energy developments in the Southwestern deserts