Digital Commons Network^™

Sphingolipids Containing Very-Long-Chain Fatty Acids Define A Secretory Pathway For Specific Polar Plasma Membrane Protein Targeting In Arabidopsis, Jennifer E. Markham, Diana Molino, Lionel Gissot, Yannick Bellec, Kian Hématy, Jessica Marion, Katia Belcram, Jean-Christophe Palauqui, Béatrice Satiat-Jeunemaître, Jean-Denis Faure

Department of Biochemistry: Faculty Publications

Sphingolipids are a class of structural membrane lipids involved in membrane trafficking and cell polarity. Functional analysis of the ceramide synthase family in Arabidopsis thaliana demonstrates the existence of two activities selective for the length of the acyl chains. Very-long-acyl-chain (C > 18 carbons) but not long-chain sphingolipids are essential for plant development. Reduction of very-long-chain fatty acid sphingolipid levels leads in particular to auxin-dependent inhibition of lateral root emergence that is associated with selective aggregation of the plasma membrane auxin carriers AUX1 and PIN1 in the cytosol. Defective targeting of polar auxin carriers is characterized by specific aggregation of Rab-A2a– …

Savvy About Succulents, Mardel Runnels, Jessica Paulisich

Agricultural Education and Communication

The purpose of this project was to create an educational booklet explaining that succulents make fantastic SAE projects. The booklet was designed to be helpful to both high school students and high school instructors. All students enrolled in agriculture programs have projects known as SAE’s. This booklet includes a variety of topics that are relevant to having an SAE project. All information was based on industry statistics and information gained from courses. The booklet can be distributed both electronically and in a print version. The major consideration in the design of this project was to develop a booklet that would …

Population Dynamics Of The Western Prickly Pear, Opuntia Macrorhiza (Cactaceae), Kathleen H. Keeler, Brigitte Tenhumberg

School of Biological Sciences: Faculty Publications

Although most cacti that have been studied are long lived, following individually marked plants in Boulder County, Colorado, for >7 years, we determined that average life span of Opuntia macrorhiza, the western prickly pear, is 3 years. A few individuals probably live >10 years. Vegetative reproduction, produced by rooting of cladodes, exceeded reproduction by germination and establishment from seeds. Both types of new recruits, from vegetative reproduction and seeds, had higher death rates than established plants. Size and frequency of flowering increased with age, although size both increased and decreased, sometimes dramatically, between years. Flowering correlated more strongly with …

Habitat Selection By Female Swift Foxes (Vulpes Velox) During The Pup- Rearing Season, Indrani Sasmal, Jonathan A. Jenks, Troy W. Grovenburg, Shubham Datta, Greg. M. Schroeder, Robert W. Klaver, Kevin M. Honness

The Prairie Naturalist

The swift fox (Vulpes velox) was historically distributed in western South Dakota including the region surrounding Badlands National Park (BNP). The species declined during the mid-1800s, largely due to habitat loss and poisoning targeted at wolves (Canis lupis) and coyotes (C. latrans). Only a small population of swift foxes near Ardmore, South Dakota persisted. In 2003, an introduction program was initiated at BNP with swift foxes translocated from Colorado and Wyoming. We report on habitat use by female swift foxes during the pup-rearing season (May–July) in 2009. Analyses of location data from 13 radiomarked female foxes indicated disproportional use (P < 0.001) of some habitats relative to their availability within swift fox home ranges. Swift foxes used grassland (ŵ = 1.01), sparse vegetation (ŵ = 1.43) and prairie dog towns (ŵ = 1.18) in proportion to their availability, whereas they were less likely to use woodland (ŵ = 0.00), shrubland (ŵ = 0.14), pasture/agricultural-land (ŵ = 0.25) and development (ŵ = 0.16) relative to availability. Swift foxes typically are located in habitats that provide greater visibility, such as shortgrass prairie and areas with sparse vegetation; which allow detection of approaching coyotes (e.g., primary predator of swift foxes).

Distribution Of Crayfish Species In Select North Dakota Streams, Cari-Ann Hayer, Terrance L. Velazquez, Mclain S. Johnson, Brian Graeb

The Prairie Naturalist

Crayfish have an integral role in aquatic ecosystems, serving as herbivores, predators, detritivores, and prey for fishes and other aquatic and terrestrial animals (Momot 1995, Taylor et al. 1996, Martin 1997). Many crayfish populations are currently declining as a result of habitat alteration (Taylor et al. 1996) and invasive predators (e.g., rusty crayfish Orconectes rusticus [Girard; Lodge et al. 2000]). Knowledge of crayfish distribution and biology in North America and specifically North Dakota is sparse or lacking (Taylor et al. 1996) and baseline information is necessary to identify effects of anthropogenic alterations. Early inventories documented 3 crayfish species in North …

Compost Tea And Milk To Suppress Powdery Mildew (Podosphaera Xanthii) On Pumpkins And Evaluation Of Horticultural Pots Made From Recyclable Fibers Under Field Conditions, Matthew Debacco

Master's Theses

Powdery mildew (caused by Podosphaera xanthii) a common problem for vegetable growers, and the cost of controlling the disease with fungicides to the growers and the environment is high. An alternative approach for control using methods approved for organic production are sprays based on teas made from compost, both actively aerated (ACT) and non-aerated (NCT) compost teas, and sprays made from diluted milk. We evaluated these sprays for control of powdery mildew on pumpkins in field trials in Connecticut in 2008 and 2009, and in greenhouse trials in 2009. We also evaluated the compost teas and milk in the greenhouse …

Impacts Of Goat Browsing And Disease On Lilium Grayi, Gray's Lily, On Roan Mountain., Joseph Powell

Undergraduate Honors Theses

The flora of southern Appalachian high elevation balds has strong representation of northern disjuncts and regional endemics. Among the endemics, the showy Lilium grayi (Gray’s Lily), is most noteworthy for its historical significance and for a high public profile. As bald vegetation changes in response to human and natural environmental shifts, active bald management has been implemented on public lands. Among managed balds, the Roan Mountain massif supports a large population of L. grayi. The purpose of this study was to describe the demography of adult plants, compare browsed and non-browsed plots, and determine the extent to which disease …

Prescribed Fire Effects On The Summer And Fall Herbs Of Mesic Deciduous Forests, Margaret Wilder

Mahurin Honors College Capstone Experience/Thesis Projects

After years of fire suppression, high intensity forest fires were destructive to surrounding areas. Historically, fire was common in the eastern United States, but was suppressed over the past century, and recently has become a major tool in forest management. But to date, there have been no studies on the influence of fire on mesic sites in the eastern United States. Because fire is being reintroduced as a management practice, it is critical to know the influence of fire in this region. This study seeks to understand the influences of fire on summer and fall herbs in the western mesophytic …

Heat-Induced Proteome Changes In Tomato Leaves, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Yang Yong, Tara Fish, Theodore W. Thannhauser

Agricultural and Environmental Sciences Faculty Research

Three tomato (Solanum lycopersicum) cultivars [Walter LA3465 (heat-tolerant), Edkawi LA 2711 (unknown heat tolerance, salt-tolerant), and LA1310 (cherry tomato)] were compared for changes in leaf proteomes after heat treatment. Seedlings with four fully expanded leaves were subjected to heat treatment of 39/25 °C at a 16:8 h light–dark cycle for 7 days. Leaves were collected at 1200 hr, 4 h after the light cycle started. For ‘Walter’ LA3465, heat-suppressed proteins were geranylgeranyl reductase, ferredoxin-NADP (+) reductase, Rubisco activase, transketolase, phosphoglycerate kinase precursor, fructose–bisphosphate aldolase, glyoxisomal malate dehydrogenase, catalase, S-adenosyl-L-homocysteine hydrolase, and methionine synthase. Two enzymes were induced, cytosolic …

Propagating Bigtooth Maple, Melody R. Richards, Larry Rupp

CWEL Extension Fact Sheets

Native plants are playing an increasing role in sustainable landscapes that use fewer resources such as water and fertilizer. Unfortunately, many native plants are not available in the nursery trade, or if they are available they may only be found as seedling plants grown for the reclamation industry. Such plants are high quality and have a valuable role in the reclamation of disturbed sites such as fire-damaged areas. But, these plants by design are genetically diverse and have not been selected for typical horticultural traits such as flowering or fall color. In addition, plants produced for reclamation are usually not …

Geologic Composition Influences Distribution Of Microbiotic Crusts In The Mojave And Colorado Deserts At The Regional Scale, Rebecca E. Drenovsky, Nicole Pietrasiak, Jeffrey R. Johansen

Biology

Abiotic and biotic factors influencing distribution of microbiotic crusts within hot deserts, such as the Mojave and Colorado Deserts, are poorly known. Our objective was to examine microbiotic crust distribution with reference to soil and parent material characteristics as well as plant functional groups in wilderness areas of Joshua Tree National Park (JTNP). A total of 75 sites were visually assessed for crust abundance and plant community composition; soil physical and chemical factors also were measured. Microbiotic crusts of JTNP, in particular lichen and moss crusts, were not as well-developed or as widely distributed as in other arid regions of …

Generation Of Transgenic Medicago Sativa Overexpressing "Osmotin-Chitinase" Gene Chimera, Jahnavi Reddy Kancharla

Masters Theses & Specialist Projects

Medicago is widely used as a forage crop. It is often susceptible to various pathogenic infections and exhibits low growth in drought and extreme climatic conditions. In the current study, a strategy was developed for over-expressing an “Osmotin-Chitinase” gene chimera in transgenic Medicago that could potentially confer resistance to different biotic and abiotic stresses. Seed germination of several cultivars of Medicago (M. sativa ssp. sativa, M. sativa ssp. falcata, M. sativa ssp. caerulea, M. truncatula, and M. Rugosa) was tested to determine the cultivars with good germination rates. Among these, M. sativa ssp. sativa showed an …

The Western Kentucky University Teaching Gardens: Landscape Design And Establishment Plan, Claudia Stetter

Masters Theses & Specialist Projects

The purpose of this thesis is to design a landscape plan for Teaching Gardens at the Western Kentucky University Agriculture Research and Education Center. The landscape plans were selected based upon the available 5.3 acres surrounding the greenhouse, vineyard, and Exposition Center. Planning included goals and objectives, analysis of the available site, landscape design plans, and connecting the Teaching Gardens to the current curriculum. This thesis also includes related areas to the development of Teaching Gardens such as: summary of courses with related topics to Teaching Gardens, programs that will be used throughout the community, and a listing of current …

A Contribution To The Vascular Flora Of The Sequatchie Valley Within Sequatchie County, Tennessee, John Richard Evans

Masters Theses and Doctoral Dissertations

The vascular flora of the Sequatchie Valley within Sequatchie County, Tennessee was studied for three growing seasons from 2008 through 2010. The Sequatchie Valley is located within the southeastern portion of the Cumberland Plateau and is generally considered to be part of the Cumberland Plateau Physiographic Province. Nevertheless, a number of geological and ecological factors distinguish the Sequatchie Valley from the elevated Cumberland Plateau, suggesting that the floristic composition of the valley may be different from the surrounding region. Although several previous floristic studies have been conducted on the plateau surface, very little attention has been given to the Sequatchie …

The Role Of Albino3 And The Lipid Environment In Chloroplast Signal Recognition Particle Targeting, Nathan Lewis

Graduate Theses and Dissertations

Signal recognition particles (SRPs) in pro- and eukaryotes function in cotranslational targeting of nascent poplypeptides to an SRP receptor at the target membrane. A unique chloroplast SRP (cpSRP) functions post-translationally to direct light-harvesting chlorophyll-binding proteins (LHCPs) to the receptor cpFtsY at the thylakoid membrane for LHCP insertion in a process involving the integral membrane protein Albino3 (Alb3) and requiring GTP. Work here focuses on understanding cpSRP targeting events at the thylakoid membrane, specifically those involving Alb3 and the lipid environment.

We show an interaction between the novel cpSRP subunit cpSRP43 and the soluble, stromal-exposed C terminus of Albino3 (Alb3-Cterm). We …

Nebline, May 2011

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nature, Gardening & Science Come Together in the Butterfly Garden

Farm Views

Horticulture

Food & Fitness

Home & Family Living

Environmental Focus

Urban Agriculture

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Summaries Of Arkansas Cotton Research 2010, Derrick M. Oosterhuis

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Evaluating Commercially Available Diagnostic Tests For The Detection Of Clavibacter Michiganensis Subsp. Nebraskensis, Cause Of Goss's Bacterial Wilt And Leaf Blight In Corn, Kevin A. Korus

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Goss’s wilt and blight of corn, caused by the bacterium Clavibacter michiganensis (Cm) subsp. nebraskensis (Cmn), is currently diagnosed by symptom identification and successful isolation onto CNS selective medium. An ELISA test kit and ImmunoStrips (Agdia^®) specific to Cm michiganensis (Cmm) reportedly give a cross-reaction with Cm subspecies. Also, the GEN III OmniLog Identification system (Biolog Inc., Hayward, CA) is said to provide accurate identification of Cm subspecies. These tests would provide a quick and inexpensive method for diagnosis of Cmn but have not been previously validated. ELISA test kits were provided by Agdia for the detection of …

Relationships Of Exotic Plant Invasions With Biological Soil Crust, Desert Pavement, And Soil Carbon In The Eastern Mojave Desert, Adria Decorte

UNLV Theses, Dissertations, Professional Papers, and Capstones

In a matter of 50 years, exotic annual plants have become widespread in the Mojave Desert, contributing to drastic landscape changes such as those caused by recent fires. Invasions by exotics threaten native Mojave Desert plant communities by altering community functions (e.g. fire regimes) and by reducing plant diversity. Because it is not practical, or even possible, to eradicate these exotics, developing effective prevention techniques is the key to controlling these invasions.

This thesis used a greenhouse experiment, a field experiment, and a correlational field study to examine the affect soil surface types have on the establishment of three exotic …

Analysis Of Rice Blast Resistance Genes In The National Small Grains Collection (Nsgc), Moytri Roychowdhury

Graduate Theses and Dissertations

Identification of blast resistance genes in rice germplasm is one of the most important activities of rice breeding programs in worldwide. The objective of this research was to characterize two major blast resistance genes, Pi-z and Pi-b, in selected rice germplasm. A simple sequence repeat DNA marker, AP5659-1 linked to the Pi-z gene, and a Pib dominant marker derived from the Pi-b gene were first used to screen a rice core collection consisting of 1700 accessions estimated to represent 70% of the genetic diversity of rice in the US National Small Grains Collection. There were 131 rice germplasm accessions with …

Comparison Of Whole-Genome Amplifications For Microsatellite Genotyping Of Rotylenchulus Reniformis, R. S. Arias, Salliana R. Stetina, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Currently, a large number of microsatellites are available for Rotylenchulus reniformis (reniform nematode); however, two barriers exist for genotyping samples from different geographical areas. The limited amount of nucleic acids obtained from single nematodes which would require their multiplication to obtain enough DNA for testing; and the strictly regulated transport of live samples and multiplication in greenhouse for being a plant pathogen. Whole-genome amplification (WGA) of samples consisting of one and five dead gravid females with their associated egg masses was successfully performed on disrupted tissue using three commercial kits. DNA yield after WGA ranged from 0.5 to 8 μg …

Simultaneous Optimization Of Macronutrient Factors In Plant Tissue Culture Using Turmeric (Curcuma Longa L.) As A Model, Sean Halloran

All Theses

Murashige and Skoog 1962 performed one-factor-at-a-time (OFAT) experimentation in order to regenerate tobacco callus on semi-solid agar medium. This work became an established medium for tissue culture micropropagation and experimentation. Micropropagation is done in niche markets with herbaceous perennials, among other crops, and the optimization of various inputs to produce maximal responses is a necessary step towards process development. This current study of macronutrient factors simultaneously altered media volume, amount of tissue (plants per vessel), sucrose, nitrogen (as NO3- and NH4+ ions), and K+ in a d-optimal design space with only 55 experimental units (including 5 true replicates). The first …

First Microsatellites From Spodoptera Frugiperda (Lepidoptera: Noctuidae) And Their Potential Use For Population Genetics, R. S. Arias, Carlos A. Blanco, Maribel Portilla, Gordon L. Snodgrass, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This is the first report of sequence-specific microsatellite markers (simple sequence repeats [SSRs]) of fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), an economically important pest of crops on the Americas.Weisolated 192 microsatellite markers by using pyrosequencing and screened 15 individuals from eight isofamilies collected from three geographical areas: Puerto Rico (PR), Texas (TX), and Mississippi (MS). Isofamilies resistant to Cry toxins from Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) also were included. Cluster analysis was performed to determine the potential use of these SSRs in discriminating populations, and colonies were grouped with a reliability of 100% estimated by bootstrap. In this …

An Ecological, Genetic, And Reproductive Study Of Big Bluestem (Andropogon Gerardii) Populations In The Carolinas, Robert Tompkins

All Dissertations

ABSTRACT
AN ECOLOGICAL, GENETIC, AND REPRODUCTIVE STUDY OF BIG BLUESTEM (ANDROPOGON GERARDII) POPULATIONS IN THE CAROLINAS
Andropogon gerardii (Big Bluestem) is a dominant grass of the North American tallgrass prairie and is also found in remnant populations in the eastern U.S., including North and South Carolina. This research included a systematic study of the ecology, genetics, and reproductive potential within and among A. gerardii populations in the Carolinas. Floristic composition and edaphic (soil) features were characterized for eight A. gerardii populations across various physiographic regions of North and South Carolina. A total of 362 quadrats (1 m x 1 m) …

Evaluating Fertilizer Rate, Crop Rotation And Trap Crops For Effects On Onion Growth And Yield, Soil Health, Thrips Densities And Iris Yellow Spot Virus Incidence, Kristine R. Buckland

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Onion production in the United States is seriously affected by the tospovirus Iris Yellow Spot (IYSV), whose symptoms include lenticular-shaped lesions that reduce photosynthesis and bulb yield. Thrips tabacai Lindeman, onion thrips (OT), is the only known vector of the disease and a primary arthropod pest of onion. Frequent insecticide applications, increasing resistance in OT populations to insecticides, high nitrogen (N) fertilization rates and loss of yield to disease and insect pressure threaten sustainable onion production. The objectives of this study were to identify crop management strategies to enhance onion productivity while suppressing OT and IYSV. Three fertilizer rates and …

Process-Based Management Of Downy Brome In Salt Desert Shrublands: Assessing Pre- And Post- Rehabilitation Soil And Vegetation Attributes, Merilynn Carol Hirsch

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

As an ecosystem driver, downy brome (Bromus tectorum L.) presents obstacles to land rehabilitation efforts, including restoring desirable species cover. Because damaged ecosystems may have crossed both abiotic and biotic thresholds, ecologically-based control strategies may assist with altering successional trajectories and restoring desirable plant species. My thesis research had three objectives: 1) assess soil and vegetation relationships in degraded salt desert ecosystems prior to implementing downy brome control treatments, 2) determine the effects of control treatments on soil properties and resident plant species, and 3) evaluate the relative importance of shrubland soil type, herbicide type, and herbicide rate on seedling …

Factors Affecting Greater Sage-Grouse (Centrocercus Urophasianus) Survival And Movement In South-Central Utah, Danny Caudill

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse are the largest North American grouse species and are dependent on sagebrush for survival. Sage-grouse populations have declined throughout the west. Habitat fragmentation and degradation are likely the main causes of declining populations, and concern has lead to the petitioning for the sage-grouse to be listed under the Endangered Species Act. Survival of adult and juvenile sage-grouse is thought to be limiting population growth. However, survival of juvenile sage-grouse is poorly understood. I aimed to improve the knowledge gap regarding juvenile sage-grouse survival. With improved knowledge of juvenile survival, management actions can be employed to benefit sage-grouse populations. …

Establishing Weed Prevention Areas And Evaluating Their Impact, Stephanie Christensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The spread of invasive weeds continues to be a serious economic and environmental threat. Weed prevention has the potential to stop weeds before they become well established in an area conserving time, energy, and resources. Unfortunately, weed prevention is often overlooked. Weed Prevention Areas (WPAs) are a relatively new tool developed to help improve the application of weed prevention. They are cooperatively managed areas that focus on implementing prevention and early detection strategies at a community level. The purpose of this research was to establish baseline data that will be used to evaluate the overall effectiveness of the WPA concept, …

Pathogen Induced Hypersensitive Response And Systemic Acquired Resistance In The Moss Amblystegium Serpens, Collin Elliot Bowman

Undergraduate Honors Thesis Collection

The moss Amblystegium serpens and the pathogenic fungus Pythium irregulare were used to study plant-pathogen interactions in a non-vascular plant. The major findings in this report include that P. irregulare does infect A. serpens but entire moss death was not noted, 25°C is the optimum temperature for P. irregulare appressoria formation, most appressoria were found on A. serpens leaves, and the SAR response within A. serpens seems to be reducing the number of P. irregulare penetrated moss cells. Future research on the pathogen defense mechanisms of A. serpens is important and promising. When we elucidate these complex chemical processes in …

An Exploration Of Pharmacognosy And Its Potential In The Multi-Faceted Solution Required To Address Hiv/Aids In Sub-Saharan Africa, Alexandra Sassi

Pell Scholars and Senior Theses

This work has both both biological and anthropoligcal foci. It addresses research done on the medicinal properties of plants and how this research could make positive contributions to the current social and biological issues surrounding the HIV/AIDS epidemic in sub-Saharan Africa.