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The Space Age Sugar Shack, Stephen G. Saupe

Biology Faculty Publications

No abstract provided.

Proceedings Of The 40th Annual Meeting, Southern Soybean Disease Workers (March 13-14, 2013, Pensacola Beach, Florida), Clayton A. Hollier, Tom Allen, Edward J. Sikora, Myra Purvis, Danise Beadle, Stephen R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern United States Soybean Disease Loss Estimates for 2012. Compiled by SR Koenning

Update on Detection and Management of QoI Fungicide Resistant Cercospora sojina, the Causal Agent of Frogeye Leaf Spot in Soybean. C Bradley, G Zhang, V Chapara, R Ming, F Zeng, H Young Kelly, and M Newman

Identification of Soybean Genotypes to Cercopsora sojina by Field Screening and Molecular Markers. A Mengistu and R Mian

Soybean Vein Necrosis Virus. D Hershman

Single Applications of Triazole Fungicides at R1 for Management of Cercospora Leaf Blight and Rust in Soybean. RW Schneider, CL Robertson, BM Ward, and EC …

Nebline, March 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Dryland Farming in a Drought

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

First Bacterial Endosymbionts Found In The Phylum Ascomycota, Eileen Elizabeth Fitzpatrick

Dissertations and Theses

Organisms belonging to the Kingdom Fungi are known to occupy a wide variety of ecological niches and are found globally in virtually all environments. Two members of the smallest of the fungal phylum, the Zygomycota, have also been found to harbor intercellular bacteria initially described as being from or closely related to organisms from the Genus Burkholderia. In this study two microaerophilic members of the species Verticilium from the phyla Ascomycota were characterized. Both appear to carry two bacterial endosymbionts. This is the first evidence of bacterial endosymbionts found within a member of the Ascomycota. Through the use of fluorescent …

Plant-Symbiotic Fungi As Chemical Engineers: Multi-Genome Analysis Of The Clavicipitaceae Reveals Dynamics Of Alkaloid Loci, Christopher L. Schardl, Carolyn A. Young, Uljana Hesse, Stefan G. Amyotte, Kalina Andreeva, Patrick J. Calie, Damien J. Fleetwood, David C. Haws, Neil Moore, Birgitt Oeser, Daniel G. Panaccione, Kathryn K. Schweri, Christine R. Voisey, Mark L. Farman, Jerzy W. Jaromczyk, Bruce A. Roe, Donal M. O'Sullivan, Barry Scott, Paul Tudzynski, Zhiqiang An, Elissaveta G. Arnaoudova, Charles T. Bullock, Nikki D. Charlton, Li Chen, Murray Cox, Randy D. Dinkins, Simona Florea, Anthony E. Glenn, Anna Gordon, Ulrich Güldener, Daniel R. Harris, Walter Hollin, Jolanta Jaromczyk, Richard D. Johnson, Anar K. Khan, Eckhard Leistner, Adrian Leuchtmann, Chunjie Li, Jinge Liu, Jinze Liu, Miao Liu, Wade Mace, Caroline Machado, Padmaja Nagabhyru, Juan Pan, Jan Schmid, Koya Sugawara, Ulrike Steiner, Johanna E. Takach, Eiji Tanaka, Jennifer S. Webb, Ella V. Wilson, Jennifer L. Wiseman, Ruriko Yoshida, Zheng Zeng

Plant Pathology Faculty Publications

The fungal family Clavicipitaceae includes plant symbionts and parasites that produce several psychoactive and bioprotective alkaloids. The family includes grass symbionts in the epichloae clade (Epichloë and Neotyphodium species), which are extraordinarily diverse both in their host interactions and in their alkaloid profiles. Epichloae produce alkaloids of four distinct classes, all of which deter insects, and some-including the infamous ergot alkaloids-have potent effects on mammals. The exceptional chemotypic diversity of the epichloae may relate to their broad range of host interactions, whereby some are pathogenic and contagious, others are mutualistic and vertically transmitted (seed-borne), and still others vary in pathogenic …

Low Concentrations Of Silver Nanoparticles In Biosolids Cause Adverse Ecosystem Responses Under Realistic Field Scenario, Benjamin P. Colman, Christina L. Arnaout, Sarah Anciaux, Claudia K. Gunsch, Michael F. Hochella Jr., Bojeong Kim, Gregory V. Lowry, Bonnie M. Mcgill, Brian C. Reinsch, Curtis J. Richardson, Jason M. Unrine, Justin P. Wright, Liyan Yin, Emily S. Bernhardt

Plant and Soil Sciences Faculty Publications

A large fraction of engineered nanomaterials in consumer and commercial products will reach natural ecosystems. To date, research on the biological impacts of environmental nanomaterial exposures has largely focused on high-concentration exposures in mechanistic lab studies with single strains of model organisms. These results are difficult to extrapolate to ecosystems, where exposures will likely be at low-concentrations and which are inhabited by a diversity of organisms. Here we show adverse responses of plants and microorganisms in a replicated long-term terrestrial mesocosm field experiment following a single low dose of silver nanoparticles (0.14 mg Ag kg⁻¹ soil) applied via a …

Plants And Plant Products Useful For Biofuel Manufacture And Feedstock, And Methods Of Producing Same, Seth Debolt, Darby Harris, Jozsef Stork

Horticulture Faculty Patents

A method of processing plant cellulose includes providing plant cellulose that is from a plant expressing a CESA polypeptide variant having at least one amino acid mutation in its carboxy-terminal transmembrane region; and saccharifying the plant cellulose to produce fermentable sugars. The method can also include fermenting the fermentable sugars to produce alcohol. A method of producing a plant having beneficial saccharification properties includes introducing into a plant a polynucleotide encoding a CESA polypeptide variant having at least one amino acid mutation in its carboxy-terminal transmembrane region; and expressing in the plant the CESA polypeptide variant, wherein plant cellulose of …

Estimated Losses Of Plant Biodiversity Across The U.S. From Historical N Deposition From 1985—2010., Christopher M. Clark, Philip E. Morefield, Frank S. Gilliam, Linda H. Pardo

Biological Sciences Faculty Research

Although nitrogen (N) deposition is a significant threat to herbaceous plant biodiversity worldwide, it is not a new stressor for many developed regions. Only recently has it become possible to estimate historical impacts nationally for the United States. We used 26 years (1985–2010) of deposition data, with ecosystem-specific functional responses from local field experiments and a national critical loads (CL) database, to generate scenario-based estimates of herbaceous species loss. Here we show that, in scenarios using the low end of the CL range, N deposition exceeded critical loads over 0.38, 6.5, 13.1, 88.6, and 222.1 million ha for the Mediterranean …

Mechanisms Of Signal Transduction By Ethylene: Overlapping And Non-Overlapping Signalling Roles In A Receptor Family, Samina N. Shakeel, Xiaomin Wang, Brad M. Binder, G. Eric Schaller

Dartmouth Scholarship

The plant hormone ethylene regulates growth and development as well as responses to biotic and abiotic stresses. Over the last few decades, key elements involved in ethylene signal transduction have been identified through genetic approaches, these elements defining a pathway that extends from initial ethylene perception at the endoplasmic reticulum to changes in transcriptional regulation within the nucleus. Here, we present our current understanding of ethylene signal transduction, focusing on recent developments that support a model with overlapping and non-overlapping roles for members of the ethylene receptor family. We consider the evidence supporting this model for sub-functionalization within the receptor …

Mammoth Cave National Park Backcountry Trail And Stream Monitoring, 2009-2012, Larry Johnson, Student Conservation Association Conservation

Mammoth Cave Research Symposia

This project conducted an assessment of backcountry trail conditions on the north side of the Green River in Mammoth Cave National Park during summers of 2009-2012. The project included assessing physical parameters of trails, (width, depth, etc.) and conducting an assessment of water quality of streams in proximity of backcountry trails. The project was conducted by Student Conservation Association resource assistants (12 weeks each) supervised by a natural resource specialist from the Mammoth Cave Division of Science and Resources Management. Trail assessment procedures were based on techniques developed by Jeff ery L. Marion, Ph.D, USGS, Virginia Tech (et al.) and …

Use Of Sorption Isotherms To Improve The Efficacy Of The Storm-Water Filters, Hung-Wai Ho, Rick Toomey

Mammoth Cave Research Symposia

Sorption has been widely used as an inexpensive and environmental friendly water treatment technology. A large variety of adsorbents with different adsorption mechanisms have drawn interests, and combinations of adsorbents will enhance sorption of mixed solutions. However, current sorption research tends to focus on single material. The objective of this study was to develop sorption isotherms for ZPG®, (Zeolite, Perlite, Granular Activated Carbon), used in a stormwater filter cartridge. Contaminants of concern include Cu2+ and quaternary ammonia compounds (QAC). Adsorption isotherms were established for Cu2+ and QAC, and the best fit for the isotherm data was a Langmuir isotherm for …

Potential Effects Of Hydrogen Sulfide And Hydrocarbon Seeps On Mammoth Cave Ecosystems, Rick Olson

Mammoth Cave Research Symposia

Recently, Mammoth Cave Guide Bruce Hatcher reported what appeared to be contaminated water seeping into Marianne’s Pass. I visited the site and concluded that it was not likely due to pollution, and that it was a natural phenomenon. To be sure, I sampled the water for fecal coliform and E. coli analyses since sewer lines are in the area. The results were <1 mpn/100ml in both cases. A sulfur spring is mentioned at this site in Rambles in the Mammoth Cave, which was published in 1845, and this predates any well drilling that could lead to hydrocarbon and sulfide pollution. Another line of evidence that the seeps are natural is that they are all deeply weathered into the bedrock, which takes a long time. The seeps support thousands of springtails, which feed on the microbial mats, and crickets are common as well. A possible effect on biodiversity will be discussed.

Antibiotic Resistance And Substrate Utilization By Bacteria Affi Liated With Cave Streams At Diff Erent Levels Of Mammoth Cave, Petra Byl, Shannon R. Trimboli, Rick Toomey, Jacob Byl, David Solomon, Tom Byl

Mammoth Cave Research Symposia

Located in south-central Kentucky, Mammoth Cave is one of the most unique National Parks in the United States. The surface landscape includes complex relationships between the flora and fauna along with human influences. However, the primary ecological focus is concealed below ground. Over four-hundred miles of cave passages, created by fl owing groundwater over millions of years, host a variety of macro and micro organisms. The Green River has cut into the limestone formation over geologic time, creating a complex network of passages that are stacked, one below the other, with the newer levels of cave lying near the bottom. …

History Of Crawling Tours At Mammoth Cave, Janet Bass Smith

Mammoth Cave Research Symposia

Currently three crawling tours are offered at Mammoth Cave: Wild Cave (six hours) for visitors ages sixteen and older, Introduction to Caving (three hours and 15 minutes) for visitors ages 10 and older (under 18 must be with an adult), and Trog (two hours and 30 minutes) for children only, ages eight through twelve. These tours are discussed in detail below. Maps for each tour are included at the end of the paper. This history relates the beginnings of each tour.

Evaluation Of Stormwater Filters At Mammoth Cave National Park, Kentucky, 2011-12, Sean Mcmillian, Ashley West, David Solomon, Roger Diehl, Victor Roland, Irucka Embry, Rick Toomey

Mammoth Cave Research Symposia

Studies in the 1970s found potentially toxic levels of metals entering Mammoth Cave’s underground streams through storm recharge. Additional studies confirmed that stormwater from parking lots and buildings fl owed rapidly into critical cave habitats. The Park’s management responded to these findings by installing storm runoff filter systems on the most heavily used parking lots in 2001. The Park entered an agreement (2010-12) with Tennessee State University, the USGS, and WKU-Mammoth Cave International Center for Science and Learning to evaluate the filter systems to determine if they were removing hazardous compounds from stormwater runoff . The objective of this study …

Effects Of Prescribed Fire On Mammoth Cave National Park’S Oak-Hickory Vegetation, Jesse A. Burton

Mammoth Cave Research Symposia

Mammoth Cave National Park contains a spectacular suite of plant communities; many of which are dependent on wildland fire as a disturbance process for their preservation. Over a third of the park is dominated by oak-hickory forests and woodlands. Fire is a fundamental process in the development and maintenance of this important community type. Since the park’s first prescribed fire in 2002, 16,700 acres of forest, woodlands, and barrens have been treated with prescribed fire. Initial goals for the prescribed fires were to reduce the density of tree saplings in the understory and increase the cover of herbaceous herbs in …

Archaeological Evidence Of Historic Mining At Forestville Saltpeter Cave (15ht94), Hart County, Kentucky, Darlene Applegate, Emily L. Duke

Mammoth Cave Research Symposia

Forestville Saltpeter Cave is 1.5 km east of Mammoth Cave National Park on the WKU Green River Preserve. Historically, miners removed sediment from the cave to extract saltpeter for the production of gunpowder. Though dozens of peter-mining sites are known in western Kentucky, Forestville Saltpeter Cave is only the third site investigated by archaeologists. Intensive surface survey documented evidence of mining activity in the cave: working bays, rock piles, tally and other marks, tool marks, inscriptions, and lighting material. The mining likely occurred during the early nineteenth century. The operations were extensive throughout all passages and extracted 856 cu m …

The Cave Beetle Neaphaenops Tellkampfi Erichson: Relationships Within And Among Related Genera Using Molecular Data, T. Keith Philips, Elise Valkanas, Kurt Helf

Mammoth Cave Research Symposia

Studies of North American cave beetle systematics have been based primarily on morphology. This project analyzes the relationships and validity of the four subspecies of the monotypic Neaphaenops based on monophyly, as well as relationships with the remaining four eastern N.A. cave beetle genera (Pseudanophthalmus, Nelsonites, Darlingtonea, and Ameroduvalius) using molecular methods. This study utilized 39 beetle samples collected from 27 Kentucky caves and one outgroup accessed from GenBank. Evidence for phylogenetic hypotheses is based on sequences of one nuclear protein-coding gene (arginine kinase) and one mitochondrial gene (CO1). Analyses support Neaphaenops as sister to all other taxa. One subspecies …

Influences Of A Cladophora Bloom On The Diets Of Amblema Plicata And Elliptio Dilatata In The Upper Green River, Kentucky, Jennifer Yates, Scott Grubbs, Albert Meier, Michael Collyer

Mammoth Cave Research Symposia

Freshwater mussels are the most imperiled group of freshwater invertebrates globally. Recent research suggests a better understanding of mussel feeding ecology may facilitate and improve conservation efforts. The use of stable isotopes is becoming an increasingly common method to study aquatic food webs. Carbon (C) and nitrogen (N) are two of the most frequently employed elements in food web studies. Differences in natural abundance of 13C/12C can indicate which food sources are the basal sources of carbon incorporated into a consumer’s tissue, while the ratio of 15N /14N provides a method of assessing trophic position within a food web. Attached …

Using Lidar To Link Forest Canopy Structure With Bat Activity And Insect Occurrence: Preliminary Findings, Luke E. Dodd, Nicholas S. Skowronski, Matthew B. Dickinson, Michael J. Lacki, Lynne K. Rieske

Mammoth Cave Research Symposia

Bats are an imperiled, yet ecologically-important group of vertebrate predators. Our ongoing research focuses on testing hypotheses about the relationships between the effects of fire on canopy structure and insect prey availability, and how these factors relate to use of foraging space by bats during the pre- and post-hibernation periods at Mammoth Cave National Park (MCNP). LiDAR-derived data (October 2010) were intersected with spatially explicit sampling of bat and insect populations (2010-2011) in order to characterize relationships between canopy structure, insect abundance, and bat activity. A canonical correspondence analysis for bat data suggested that forest canopy structure has a strong …

A Functional Visual System In The Cave Beetle Ptomaphagus Hirtus, Markus Friedrich, Rui Chen, Elke K. Buschbeck, Stewart Peck

Mammoth Cave Research Symposia

Cave species exhibit a suite of dramatic differences in comparison to their surface living relatives, commonly referred to as troglomorphy (Christiansen 2005). One hallmark feature of troglomorphy is the severe reduction or complete loss of eyes and functional vision. The two most abundant cave beetle species in Mammoth cave, the 2-3 mm small carrion beetle Ptomaphagus hirtus (Packard 1888; Peck 1973; Peck 1975; Tellkampf 1844) and the 6-8 mm long predatory ground beetle Neaphaenops tellkampfii are good examples of this (Barr 1979). P. hirtus tends to hide in crevices and usually goes unnoted to the regular visitor of Mammoth cave. …

Disjunct Eastern Hemlock Populations Of The Central Hardwood Forests: Ancient Relicts Or Recent Long Distance Dispersal Events?, F. Collin Hobbs, Keith Clay

Mammoth Cave Research Symposia

Eastern hemlock (Tsuga canadensis) is an evergreen conifer with a contiguous distribution extending from the southern Appalachian Mountains north to Nova Scotia and west across the Great Lakes region. Eastern hemlock is threatened with extirpation from much of this range by an introduced pest, the hemlock woolly adelgid (Orwig et al. 2002). In addition to the contiguous distribution, many small, isolated populations are located within the central hardwood forest region of Kentucky, Indiana, and Ohio (Braun 1928, Potzger and Friesner 1937, Van Stockum 1979). These disjunct populations form clearly delineated, often monospecific stands associated with unique natural features such as …

Landscape Genetics Of The Marbled Salamander, Ambystoma Opacum, In A Nationally Protected Park, Kevin Tewell, Jarrett Johnson

Mammoth Cave Research Symposia

Landscape features may influence the patterns of migration and dispersal of amphibian species and create genetic structure. A primary goal of landscape genetics is to analyze these influences in order to make more informed management decisions. We sampled larvae from 50 breeding ponds within the boundaries of Mammoth Cave National Park and genotyped 12 individuals per pond at 10 microsatellite loci to estimate gene fl ow between ponds. We used GIS layers of habitat types to conduct a least-cost path analysis and determine the relative cost of movement through each habitat type. We were interested in answering two questions: does …

Mammoth Cave National Park's 10th Research Symposium: Celebrating Diversity Of Research In The Mammoth Cave Region, Shannon Trimboli ,Editor

Mammoth Cave Research Symposia

No abstract provided.

Traversing Swanton Road, 12th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

Control Of Flower Size, Beth A. Krizek, Jill T. Anderson

Faculty Publications

Flowers exhibit amazing morphological diversity in many traits, including their size. In addition to interspecific flower size differences, many species maintain significant variation in flower size within and among populations. Flower size variation can contribute to reproductive isolation of species and thus has clear evolutionary consequences. In this review we integrate information on flower size variation from both evolutionary and developmental biology perspectives. We examine the role of flower size in the context of mating system evolution. In addition, we describe what is currently known about the genetic basis of flower size based on quantitative trait locus (QTL) mapping in …

Regional Contingencies In The Relationship Between Aboveground Biomass And Litter In The World’S Grasslands, Lydia R. O'Halloran, Elizabeth T. Borer, Eric W. Seabloom, Sarah Hobbie, Andrew S. Macdougall, Elsa E. Cleland, Rebecca L. Mcculley, W. Stan Harpole, Kirsten S. Hofmockel, Wei Li, Nicole M. Decrappeo, Chengjin Chu, Guozhen Du, Jonathan D. Bakker, Kendi F. Davies, Brett A. Melbourne, Jennifer Firn, Nicole Hagenah, Johannes M. H. Knops, John W. Morgan, John L. Orrock, Suzanne M. Prober, Carly J. Stevens

Plant and Soil Sciences Faculty Publications

Based on regional-scale studies, aboveground production and litter decomposition are thought to positively covary, because they are driven by shared biotic and climatic factors. Until now we have been unable to test whether production and decomposition are generally coupled across climatically dissimilar regions, because we lacked replicated data collected within a single vegetation type across multiple regions, obfuscating the drivers and generality of the association between production and decomposition. Furthermore, our understanding of the relationships between production and decomposition rests heavily on separate meta-analyses of each response, because no studies have simultaneously measured production and the accumulation or decomposition of …

Identifying Compounds To Help Control Bed Bugs, Mark Feldlaufer

Agricultural Research Magazine

When planning an attack on bothersome pests, it’s a good idea to know your enemies, especially if they happen to be bed bugs. These small, flat, blood-feeding insects—an uncommon problem since the 1950s—have once again become a menace in homes, apartments, hotels, shelters, and even places of work in the last 10 years.

Despite extensive efforts to determine whether bed bugs transmit disease-causing pathogens, no evidence indicates that they are a problem other than their biting, blood feeding, and dirty habits. However, some people have severe reactions to bed bug bites, and getting rid of the insects can be costly. …

Lifting All Boats With Organic And Conventional Research, Matt Smith

Agricultural Research Magazine

At first glance, the divide between organic and conventional farming appears to be of Grand Canyon proportions. But when it comes to research, the distance between organic and conventional farming practices is not nearly so great.

The Agricultural Research Service has a national program that specifically carries out studies aimed at helping organic farmers to compete in the marketplace by finding ways to cost-effectively produce abundant amounts of high-quality, safe products to meet consumer demands.

Consumer demand for organically produced goods has shown double-digit growth for well over a decade, providing market incentives for U.S. farmers across a broad range …

Studying Manure In Cows, In Feedlots, And In Fields, Lisa M. Durso, John Gilley, Ann Perry

Agricultural Research Magazine

Agricultural Research Service scientists at the Agroecosystems Management Research Unit in Lincoln, Nebraska, have been conducting some very thorough investigations on the microbes that dwell in cattle manure—what they are, where they thrive, where they struggle, and where they may end up. “When we look at potential pathogens that can cause foodborne illness, we need to look at the whole bacterial ecosystem,” says ARS microbiologist Lisa Durso. “For instance, some people used to think all cattle have the same bacteria in their gastrointestinal [GI] tracts. But we’ve found some big differences; so if we say, ‘Oh, it’s just manure,’ we …