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Growing South Dakota (Winter 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2013 SDSU Agricultural Experiment Station Annual Report

[Page] 2 The World’s First: Collaborative Effort Produces Multi-Hybrid Planter To Advance Precision Ag
[Page] 4 70 Years Of Data: Ranchers Benefit From Long-Term Grazing Studies
[Page] 6 Better Beef: Fetal Programming Study Analyzes Effects Of Cow Nutrition On Meat Quality
[Page] 7 Winter College News
[Page] 8 Consumer Sciences, Animal Health, Livestock Marketing: Important Research Continues In These Areas
[Page] 10 Improving Human Health: Role Of Nondigestible Fiber, Antioxidants Studied To Prevent Disease
[Page] 11 Food Safety Focus: Targeting Heat-Resistant Microbes To Improve Dairy Products
[Page] 12 Engineering …

Effects Of Cerium Oxide Nanoparticles In Cereals: Insights Into The Toxicity Mechanisms And Macromolecular Modifications, Cyren Mendoza Rico

Open Access Theses & Dissertations

Despite the inundation of studies on the interaction of engineered nanomaterials (ENMs) with plants, investigations involving complete life cycle (i.e from seedling establishment to full maturity) are still lacking. Assessments on the nutritional value of plants cultivated to full maturity in ENMs-treated soil are also missing. Cerium oxide nanoparticles (nCeO2) have significant interactions with plants; however, there are no life cycle studies yet on their implications in cereals like rice (Oryza sativa L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). These cereals are globally important crops that support the economic activity, and nutritional and health needs of billions …

Lichens Of Six Vernal Pools In Acadia National Park, Me, Usa, J. Barton, B. Ciccotelli, J. E. Gall, F. C. Olday, B. Connery, T. B. Harris, A. M. Fryday, Nishanta Rajakaruna

Faculty Publications, Biological Sciences

Whereas lichen-habitat relations have been well-documented globally, literature on lichens of vernal pools is scant. We surveyed six vernal pools at Acadia National Park on Mount Desert Island, Maine, USA for their lichen diversity. Sixty-seven species were identified, including seven species that are new reports for Acadia National Park: Fuscidea arboricola, Hypogymnia incurvoides, Lepraria finkii, Phaeographis inusta, Ropalospora viridis, Usnea flammea, and Violella fucata. Five species are considered uncommon or only locally common in New England: Everniastrum catawbiense, Hypogymnia krogiae, Pseudevernia cladonia, Usnea flammea, and Usnea merrillii. This work represents the first survey of lichens from vernal pools in Acadia …

Additional Lichen Records And Minerological Data From Metal-Contaminated Sites In Maine, I. D. Medeiros, A. M. Fryday, Nishanta Rajakaruna

Faculty Publications, Biological Sciences

No abstract provided.

Diversity And Soil-Tissue Elemental Relations Of Vascular Plants Of Callahan Mine, Brooksville, Maine, U.S.A, Margaret Mansfield, Nathaniel Pope, Glen Mittlehauser, Nishanta Rajakaruna

Faculty Publications, Biological Sciences

Metal-contaminated soils provide numerous stressors to plant life, resulting in unique plant communities worldwide. The current study focuses on the vascular plants of Callahan Mine in Brooksville, ME, USA, a Superfund site contaminated with Cu, Zn, Pb, and other pollutants. One hundred and fifty-five taxa belonging to 50 families were identified, with the Asteraceae (21%), Poaceae (11%), and Rosaceae (9%) as the most species-rich families. Ninety-six species encountered at the Mine were native to North America (62%), including 11 taxa (7%) with rarity status in at least one New England state. Fifty-one species were non-native (33%), including nine taxa (6%) …

Sarpa Salpa Herbivory On Shallow Reaches Of Posidonia Oceanica Beds, Latina Steele, Kelly M. Darnell, Just Cebrián, Jose Luis Sanchez-Lizaso

Biology Faculty Publications

Sarpa salpa herbivory on shallow reaches of Posidonia oceanica beds.— Here, we examined the temporal and small–scale spatial variability of grazing by the herbivorous fish Sarpa salpa on shallow beds of the temperate seagrass Posidonia oceanica. Herbivory intensity expressed as the percent of leaf area taken by fish bites was higher in September 2006 than in February 2007, and at 0.5 m than at 1.5 m during both sampling times. All S. salpa feeding at the shallow locations studied were juveniles, with bite sizes ranging from 0.03 to 0.62 cm2. Juveniles feeding at 1.5 m were larger in February 2007 …

Molecular Markers Reveal Narrow Genetic Base And Culturing-Associated Genetic Drift In Teretrius Nigrescens Lewis Populations Released For The Biological Control Of The Larger Grain Borer In Africa, B. A. Omondi, J. Van Den Berg, D. Masiga, F. Schulthess

All Peer-Reviewed Publications

In biological control, successful establishment of a natural enemy species depends on its adaptability in the introduced range including its ability to re-establish desired ecological interactions with the pest. These are affected by genetic parameters hitherto largely unresolved in biological control. The larger grain borer (LGB), Prostephanus truncatus, an invasive species from meso-America, is the most important post-harvest pest of maize in Africa. We studied the genetic structure of Teretrius nigrescens, a predatory beetle previously released for the control of the pest in Africa, to test the hypothesis that establishment patterns were a result of ecotype-environment mismatch and to follow …

Drought Sensitivity Of Slash Pine And Longleaf Pine Deduced By Tree Ring Analysis, Conor Madison

Honors Theses and Capstones

Annual tree rings give us the opportunity to investigate the adaptation of trees to climate and environmental changes over a long period of time. In particular, the physical characteristics of each ring (width and earlywood and late wood differentiation) can be used to reconstruct past environment conditions. Physiological responses of trees will be evaluated for two conifers species, i.e. Longleaf pine and Slash pine, giving the opportunity to compare the two species and understand how each species (Longleaf pine and Slash pine) adapt their water use to thrive in such extreme environments. Growth data will then be related to the …

Detection Of Seagrass Scars Using Sparse Coding And Morphological Filter, Ender Oguslu, Sertan Erkanli, Victoria J. Hill, W. Paul Bissett, Richard C. Zimmerman, Jiang Li, Charles R. Bostater Jr. (Ed.), Stelios P. Mertikas (Ed.), Xavier Neyt (Ed.)

OES Faculty Publications

We present a two-step algorithm for the detection of seafloor propeller seagrass scars in shallow water using panchromatic images. The first step is to classify image pixels into scar and non-scar categories based on a sparse coding algorithm. The first step produces an initial scar map in which false positive scar pixels may be present. In the second step, local orientation of each detected scar pixel is computed using the morphological directional profile, which is defined as outputs of a directional filter with a varying orientation parameter. The profile is then utilized to eliminate false positives and generate the final …

The Effects Of Herbicide On The Endangered Herb Baptisia Arachnifera And Preliminary Nutrient Survey Of Leaf Tissue And Soil, Ruth Ann C. Steinbrecher

College of Graduate Studies: Theses & Dissertations

Baptisia arachnifera (Hairy Rattleweed) is an endangered herbaceous legume that only occurs in Wayne and Brantley Counties of Georgia, United States. Many of the remaining populations exist in areas now managed for timber. This study investigated the effects of Imazapyr, an herbicide commonly used in timber management, on health and vitality of B. arachnifera under both field and greenhouse conditions. This study also analyzed leaf and soil samples from six populations of B. arachnifera to determine the nutrient content of the leaves. A recensus of a B. arachnifera population was also conducted in a site without commercial timber management. In …

Soil Microbial Community Distributions And Disease Suppressiveness In The Coastal Plain Of Georgia, Michael J. Sabula

College of Graduate Studies: Theses & Dissertations

This study compared the soil microbial communities of three vegetation types in the coastal plain of Georgia: 1. crop land actively in use for agricultural production, 2. transitional grassland in early stages of secondary succession, and 3. pristine unmanaged forest land. Microbial species diversity and quantities of microbial DNA were determined from each of these vegetation types at three separate locations near Statesboro, Georgia. Length heterogeneity PCR(LH-PCR) methods and subsequent analysis of fungal, bacterial, and metazoan communities by analysis of similarity (ANOSIM) revealed high within-group similarity by vegetation type, indicating land management intensity and vegetation cover is a strong determining …

Phenotypic Variation In Native North American And Invasive Chinese Populations Of Plantago Virginica, Teresa E. Popp

College of Graduate Studies: Theses & Dissertations

Alien plant species can cause significant economic and biological destruction by invading new environments and outcompeting native species. Through experimental research, the mechanisms by which these species invade can be better understood and applied to their management. Plantago virginica is a perennial herb, native to North America that has recently invaded China. It has been known to reduce crop growth and harbor diseases in the introduced range. A common garden study was conducted at the Georgia Southern University greenhouse, comparing twenty populations of P. virginica from both the native North American and invasive Chinese ranges. I examined if there were …

A Structure-Function Analysis Of The Arabidopsis Chloroplast Import Protein Attic20, James H. Campbell

Theses and Dissertations (Comprehensive)

Arabidopsis thaliana protein Tic20 (atTic20) is a member of the translocon at the inner envelope membrane of chloroplasts. Evidence to date suggests it is part of the main preprotein conducting aperture in the complex, but its exact role is still debated. To help characterize its role, a protocol optimizing yield and purity of recombinantly expressed atTic20 was developed, and a series of experiments was performed to examine its secondary structure and its ability to interact with chloroplast transit peptides. The attempt to increase protein yield was successful, with growth at 20^oC in the auto-inducing media ZYP-5052 showing the …

The Conservation Value Of Karst Dolines For Vascular Plants In Woodland Habitats Of Hungary: Refugia And Climate Change, Zoltán Bátori, János Csiky, Tünde Farkas, E. Anna Vojtkó, László Erdős, Dániel Kovács, Tamás Wirth, László Körmöczi, András Vojtkó

International Journal of Speleology

Limestone (karst) surfaces in Hungary are rich in dolines, in which many endangered vascular plant species occur. To date, the majority of studies dealing with doline vegetation have focused on the local rather than the landscape level, without using comparative data from other areas. However, in this study we aimed to compare the vegetation pattern and species composition of dolines under different climate regimes of Hungary with regard to regional species pools. The fieldwork was carried out between 2005 and 2012. Twenty dolines were selected in the Mecsek Mountains (southern Hungary) and nine dolines in the Aggtelek Karst area (northern …

Seagrass Canopy Photosynthetic Response Is A Function Of Canopy Density And Light Environment: A Model For Amphibolis Griffithii, John D. Hedley, Kathryn Mcmahon, Peter Fearns

Research outputs 2014 to 2021

A three-dimensional computer model of canopies of the seagrass Amphibolis griffithii was used to investigate the consequences of variations in canopy structure and benthic light environment on leaf-level photosynthetic saturation state. The model was constructed using empirical data of plant morphometrics from a previously conducted shading experiment and validated well to in-situ data on light attenuation in canopies of different densities. Using published values of the leaf-level saturating irradiance for photosynthesis, results show that the interaction of canopy density and canopy-scale photosynthetic response is complex and non-linear, due to the combination of self-shading and the non-linearity of photosynthesis versus irradiance …

Integrating Soil And Plant Tissue Tests And Using An Artificial Intelligence Method For Data Modelling Is Likely To Improve Decisions For In-Season Nitrogen Management, Andreas Neuhaus, Leisa Armstrong, Jinsong Leng, Dean Diepeveen, Geoff Anderson

Research outputs 2014 to 2021

This paper hypothesizes that there is value in combining soil, climate and plant tissue data to give more reliable advice on nitrogen top-ups in-season when compared with models that are currently available. The benefit of soil and climate data is to factor in N mineralisation and potential yield while plant test data is a more direct approach of yield estimates when considering firstly plant N uptake from the whole soil profile and secondly biomass (important yield component). Plant test data are closer to yield in time and space than soil test data, shortening the time period for any yield prognosis …

On The Application Of Genetic Probabilistic Neural Networksand Cellular Neural Networks In Precision Agriculture, Oluleye H. Babatunde, Leisa Armstrong, Jinsong Leng, Dean Diepeveen

Research outputs 2014 to 2021

This article details the effect of Gaussian smoothing parameter (spread) on the performance of Probabilistic Neural Networks (PNN). Two (2) different Genetic Algorithms (GAs) were used to optimize the PNN spread in order to avoid under and over fitting. In this work there is a novel combination of Cellular Neural Networks (CNN), Probabilistic Neural Networks (PNN) and GA to address the present challenges on automatic identification of plant species. Such problems include misclassification species of plants that are similar in shapes and image segmentation speed. In this work, GA was used in both feature selection and PNN parameter optimization. The …

Tracking Above- And Below- Ground Seed Persistence And Mortality In A Native Tallgrass Prairie Restoration, Carmen Pellish

Dissertations and Theses @ UNI

The large cost of seed and low seedling establishment rates make restoring native tallgrass prairies expensive and difficult. Tallgrass prairie restorations typically achieve seedling emergence rates of only 10%. This begs the question of what happens to the remaining 90% of seeds that do not emerge as seedlings. This thesis sought to assess or quantify the importance of seed predators and death by microorganisms or to senescence on seed survival and seedling establishment within a newly planted native tallgrass prairie restoration under typical restoration conditions. I hypothesized that small vertebrate seed predators would play be detrimental to overall seedling emergence …

Development Of Leaf Shape And Vein Homologies In Five Species Of The Genus Ipomoea (Convolvulaceae), Austin William Jones

Dissertations and Theses @ UNI

Angiosperm leaves are extremely variable in form while predominantly maintaining the function of the primary photosynthetic organ of the plant. Changes in leaf form can result from myriad physiological processes which may be influenced by ecology, physical stimuli, phylogeny, or other factors. In studying the development of divergent leaf forms among closely related species, conserved morphological elements may be identified that are not apparent in the mature form. The genus Ipomoea (Convolvulaceae) contains over 600 species and a wide range in leaf shapes. Five species, I. purpurea, I. coccinea, I. sloteri, I. quamoclit, and I. cairica show a range in …

The Movement Ecology Of Seagrasses, Kathryn Mcmahon, Kor-Jent Van Dijk, Leonardo Ruiz-Montoya, Gary A. Kendrick, Siegfried L. Krauss, Michelle Waycott, Jennifer Verduin, Ryan Lowe, John Statton, Eloise Brown, Carlos Duarte

Research outputs 2014 to 2021

A movement ecology framework is applied to enhance our understanding of the causes, mechanisms and consequences of movement in seagrasses: marine, clonal, flowering plants. Four life-history stages of seagrasses can move: pollen, sexual propagules, vegetative fragments and the spread of individuals through clonal growth. Movement occurs on the water surface, in the water column, on or in the sediment, via animal vectors and through spreading clones. A capacity for long-distance dispersal and demographic connectivity over multiple timeframes is the novel feature of the movement ecology of seagrasses with significant evolutionary and ecological consequences. The space–time movement footprint of different life-history …

Development Of A Loop-Mediated Isothermal Amplification For The Detection Of Burkholderia Glumae, Maria Alejandra Caldera

LSU Master's Theses

Bacterial panicle blight is among the three most limiting diseases of rice production in the United States. Yield loss up to 40% has been reported from rice fields severely infested with bacterial panicle blight. The disease is caused by Burkholderia glumae and B. gladioli. Although, both species produce similar symptoms and are closely related, but B. glumae strains are more aggressive and cause more severe symptoms on rice. Bacterial panicle bight is difficult to manage in the absence of effective chemical control measures. Rapid and early disease detection is needed to avoid severe yield losses. Several techniques have been developed …

Characterization Of A Novel Negative Regulator Of Toxoflavin Production, Ntpr, In The Plant Pathogen Burkholderia Glumae That Causes Bacterial Panicle Blight Of Rice, Rebecca Ann Melanson

LSU Doctoral Dissertations

Burkholderia glumae is the major causal agent of bacterial panicle blight of rice, a disease that can cause significant yield losses. This bacterial pathogen produces a number of virulence factors that contribute to disease development. The phytotoxin, toxoflavin is required for full virulence and is the most important virulence factor in B. glumae. The known regulatory cascade of toxoflavin production involves the TofI-TofR quorum-sensing system, the transcriptional activators ToxJ and ToxR, and the toxoflavin biosynthesis and transport genes. Transposon mutagenesis of toxoflavin-producing B. glumae strains generated a number of mutants with increased toxoflavin production. Several of these mutants contained …

Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva

LSU Master's Theses

Cercospora leaf blight (CLB) of soybean caused by Cercospora kikuchii is an important disease in Lousiana. Preliminary screening of foliar applications of the micronutrients Fe, Mn, Cu, Zn, B, Mo and Al, showed that Fe decreased CLB severity consistently. The objective of this work was to test the effects of commercial formulations of Fe, Manny Plex Fe and Fe EDTA (Brandt Consolidated, Springfield, IL) on leaf colonization by C. kikuchii, symptom development (blight and purple leaves), and yield. Four rates of Manny Plex Fe and four rates of Fe EDTA were applied to field plots at R5 growth stage. Leaf …

Characterization Of A Σ54-Dependent Response Regulator, Tepr, In The Rice-Pathogenic Bacterium Burkholderia Glumae And Development Of Biocontrol Strategies For Bacterial Panicle Blight Of Rice, Surendra Osti

LSU Master's Theses

Bacterial panicle blight, caused by Burkholderia glumae, is a major bacterial disease of rice in Louisiana. This bacterium contains several virulence factors required for disease development such as toxoflavin, lipase and flagella. In a genome-wide search for regulatory factors related to the virulence of B. glumae, tepR was identified as a negative regulator for toxoflavin production and found to encode a sigma 54-dependent response regulator. TepR is homologous to LuxO, a quorum-sensing signaling component of Vibrio spp. A markerless tepR deletion mutant of B. glumae 336gr-1, LSUPB401, produced more toxoflavin and showed higher lipase and protease activities compared to the …

Getting Plant Conservation Right (Or Not): The Case Of The United States, Kayri Havens, Andrea T. Kramer, Edward O. Guerrant Jr.

Environmental Science and Management Faculty Publications and Presentations

Effective plant conservation includes addressing basic needs such as information about species distribution and rarity; research, management, education, and training capacity to mitigate threats facing threatened species; policy and funding to support continued capacity and conservation; and, ultimately, a public that understands and supports the importance of plants and the need for their conservation. Coordination of plant conservation efforts is also needed to ensure that resources and expertise are used in a strategic, efficient, and effective manner.We argue that no country is currently getting plant conservation right; plants are becoming increasingly rare around the world. Plants are often not fully …

Sampling For Effective Ex Situ Plant Conservation, Edward O. Guerrant Jr., Kayri Havens, Pati Vitt

Environmental Science and Management Faculty Publications and Presentations

Sampling a whole flora or any significant subset for ex situ conservation purposes is a complex, long-term proposition. Thus, it is important to consider what constitutes an adequate sample not only for all taxa as a whole but also for each taxon individually as well as how to strategically schedule collection over time. There are five basic sampling questions: from which species to collect, from how many and which populations, from how many and which individuals, how many and what kind of propagules to collect, and, finally, at what point is the desired sample size too great for a population …

Identification Of Unknowns Within A Probabilistic System: The Diagnostic Value Of Attributes, D W. Goodall

Research outputs 2014 to 2021

Using a data base underpinned by probability considerations in which a variety of attributes, some of which may be quantitative, are recorded for a number of “operational taxonomic units” (OTUs), a key system is described by which an unnamed specimen may quickly be identified. The concept of “diagnostic power” is introduced, by which each attribute is evaluated in terms of its potential contribution to identifying the unnamed specimen. Besides coverage of different types of attributes and the introduction of “diagnostic power”, the system has the advantages of incorporating multiple values of an attribute for each OTU and offering short-cuts to …

Decision Support System Data For Farmer Decision Making, Pornchai Taechatanasat, Leisa Armstrong

Research outputs 2014 to 2021

The capacity of farmers and agricultural scientists to be able to make in-season decisions is dependent on accurate climate, soil and plant data. This paper will provide a review of the types of environmental and crop data that can be collected by sensors which can used for decision support systems (DSS) or be further interrogated for real time data mining and analysis. This paper also presents a review of the data requirements for agricultural decision making by firstly reviewing decision support frameworks and agricultural DSSs, data acquisition, sensors for data acquisition and examples of data incorporation for agricultural DSSs.

Fate Of Eucalyptus Marginata Seed From Canopy-Store To Emergence In The Northern Jarrah Forests Of Western Australia: Research To Help Improve Regeneration Following Shelterwood Treatment, Jeffrey Cargill

Theses: Doctorates and Masters

The establishment of seedling regeneration is a key process in and indicator of ecologically sustainable forest management. The availability of seed and the creation of a suitable seedbed are recognised as important factors limiting seedling recruitment. A silvicultural method commonly used across northern and eastern jarrah forest blocks is shelterwood cutting. The primary objective of treating jarrah forest to shelterwood is to promote seedling regeneration in areas lacking sufficient advanced growth. Despite the widespread and progressive implementation of the shelterwood method, its application in jarrah forest has shown varying degrees of success.

This thesis sought to investigate and better understand …

Effects Of Understory Vegetation And Litter On Plant Nitrogen (N), Phosphorus (P), N∶P Ratio And Their Relationships With Growth Rate Of Indigenous Seedlings In Subtropical Plantations, Jun Wang, Dafeng Hui, Hai Ren, Zhanfeng Liu, Long Yang

Biology Faculty Research

Establishing seedlings in subtropical plantations is very important for forest health, succession and management. Information on seedling nutrient concentrations is essential for both the selection of suitable indigenous tree species to accelerate succession of the established plantation and sustainable forest management. In this study, we investigated the concentrations of nitrogen ([N]), phosphorus ([P]), and N∶P ratio in leaves, stems and roots of seedlings of three indigenous tree species (Castanopsis chinensis, Michelia chapensis and Psychotria rubra) transplanted with removing or retaining understory vegetation and litter at two typical subtropical forest plantations (Eucalyptus plantation and native species plantation). We also measured the …